SitesBLAST
Comparing WP_057507612.1 NCBI__GCF_001431535.1:WP_057507612.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q4W6G0 Quinohemoprotein alcohol dehydrogenase ADH-IIG; ADH IIG; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
27% identity, 83% coverage: 116:789/814 of query aligns to 6:567/718 of Q4W6G0
- C138 (≠ D269) modified: Disulfide link with 139
- C139 (≠ L270) modified: Disulfide link with 138
- R144 (≠ T275) binding
- T189 (= T354) binding
- GA 205:206 (≠ NL 373:374) binding
- E207 (≠ T375) binding
- T264 (vs. gap) binding
- N284 (= N441) binding
- D329 (= D485) binding
- K356 (= K513) binding
- W415 (≠ Y607) binding
- DW 419:420 (≠ NW 611:612) binding
Sites not aligning to the query:
- 1:29 signal peptide
- 30:718 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH-IIG
- 635 binding covalent
- 638 binding covalent
- 639 binding axial binding residue
- 676 binding axial binding residue
1yiqA Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase adhiig from pseudomonas putida hk5. Compariison to the other quinohemoprotein alcohol dehydrogenase adhiib found in the same microorganism. (see paper)
27% identity, 78% coverage: 159:789/814 of query aligns to 14:538/684 of 1yiqA
- active site: E178 (≠ T375), N255 (= N441), D300 (= D485)
- binding calcium ion: E178 (≠ T375), N255 (= N441), D300 (= D485)
- binding pyrroloquinoline quinone: E63 (≠ Q213), C109 (≠ D269), C110 (≠ L270), R115 (≠ T275), T160 (= T354), G175 (≠ D372), G176 (≠ N373), A177 (≠ L374), E178 (≠ T375), T235 (vs. gap), W237 (vs. gap), K327 (= K513), D390 (≠ N611), W391 (= W612), F477 (≠ K707)
Sites not aligning to the query:
- binding heme c: 605, 606, 608, 609, 610, 623, 626, 630, 634, 637, 638, 642, 645, 646, 647, 648, 650
- binding pyrroloquinoline quinone: 542
4aahA Methanol dehydrogenase from methylophilus w3a1 (see paper)
27% identity, 76% coverage: 168:789/814 of query aligns to 14:527/571 of 4aahA
- active site: E171 (= E378), N255 (= N441), D297 (= D485)
- binding calcium ion: E171 (= E378), N255 (= N441)
- binding pyrroloquinoline quinone: E55 (≠ Q213), C103 (≠ R261), C104 (= C262), R109 (≠ T275), S168 (≠ T375), A170 (≠ G377), E171 (= E378), W237 (= W423), R324 (≠ K513), N387 (= N611), W467 (≠ M727)
Sites not aligning to the query:
8gy2A Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
30% identity, 47% coverage: 155:533/814 of query aligns to 8:355/723 of 8gy2A
- binding calcium ion: E181 (≠ T375), N263 (= N441), D308 (= D485)
- binding heme c: D104 (= D269)
- binding pyrroloquinoline quinone: C107 (≠ A272), C108 (≠ L273), D163 (≠ T354), G179 (≠ N373), A180 (≠ L374), E181 (≠ T375), W245 (= W423), N263 (= N441), D308 (= D485), K335 (= K513)
Sites not aligning to the query:
- binding heme c: 618, 619, 622, 623, 633, 634, 636, 639, 652, 660, 662, 665
- binding pyrroloquinoline quinone: 398, 489
O05542 Alcohol dehydrogenase (quinone), dehydrogenase subunit; ADH; Alcohol dehydrogenase (quinone), acceptor subunit; Alcohol dehydrogenase (quinone), subunit I; Ethanol:Q2 reductase; G3-ADH subunit I; Quinohemoprotein alcohol dehydrogenase; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
30% identity, 47% coverage: 155:533/814 of query aligns to 42:389/757 of O05542
Sites not aligning to the query:
- 1:34 signal peptide
- 35 modified: Pyrrolidone carboxylic acid
Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
25% identity, 77% coverage: 160:789/814 of query aligns to 32:543/690 of Q8GR64
- E81 (≠ Q213) binding
- C127 (= C262) modified: Disulfide link with 128
- C128 (≠ L270) modified: Disulfide link with 127
- R133 (≠ T275) binding
- T177 (= T354) binding
- GA 193:194 (≠ NL 373:374) binding
- E195 (≠ T375) binding
- T252 (vs. gap) binding
- N272 (= N441) binding
- D317 (= D485) binding
- K344 (= K513) binding
- NW 404:405 (= NW 611:612) binding
Sites not aligning to the query:
- 1:22 signal peptide
- 23:690 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH IIB
- 547 binding
- 613 binding covalent
- 616 binding covalent
- 617 binding axial binding residue
- 655 binding axial binding residue
1kv9A Structure at 1.9 a resolution of a quinohemoprotein alcohol dehydrogenase from pseudomonas putida hk5 (see paper)
25% identity, 77% coverage: 160:789/814 of query aligns to 10:521/664 of 1kv9A
- active site: E173 (≠ T375), N250 (= N441), D295 (= D485)
- binding acetone: E173 (≠ T375), D295 (= D485)
- binding calcium ion: E173 (≠ T375), N250 (= N441), D295 (= D485)
- binding heme c: A101 (≠ E254), R102 (≠ S255)
- binding pyrroloquinoline quinone: E59 (≠ Q213), C105 (= C262), C106 (≠ L270), R111 (≠ T275), T155 (= T354), G170 (≠ D372), A172 (≠ L374), E173 (≠ T375), T230 (vs. gap), W232 (= W423), K322 (= K513), N382 (= N611), W383 (= W612), W460 (≠ T726)
Sites not aligning to the query:
- binding heme c: 590, 591, 594, 595, 605, 606, 608, 611, 615, 619, 623, 631, 633, 636
- binding pyrroloquinoline quinone: 525
7o6zB Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
26% identity, 77% coverage: 167:789/814 of query aligns to 13:541/588 of 7o6zB
- binding methanol: E173 (≠ D372), W263 (= W447), D314 (= D485)
- binding Neodymium Ion: E173 (≠ D372), N259 (= N441), D314 (= D485), D316 (= D487)
- binding pyrroloquinoline quinone: E55 (= E211), C105 (≠ T277), C106 (≠ A278), R111 (≠ T283), T155 (≠ A342), G170 (≠ R369), G171 (≠ L370), D172 (≠ N371), E173 (≠ D372), W241 (= W423), D316 (= D487), R341 (≠ K513), D403 (≠ N611), W481 (≠ M727)
Sites not aligning to the query:
7o6zA Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
26% identity, 77% coverage: 167:789/814 of query aligns to 13:541/588 of 7o6zA
Sites not aligning to the query:
Q46444 Quinohemoprotein alcohol dehydrogenase; QH-ADH; Alcohol dehydrogenase (azurin); PQQ-containing alcohol dehydrogenase; PQQ-dependent ADH; Quinohaemoprotein ethanol dehydrogenase type I; QH-EDHI; EC 1.1.9.1 from Comamonas testosteroni (Pseudomonas testosteroni) (see 3 papers)
25% identity, 77% coverage: 167:789/814 of query aligns to 59:571/708 of Q46444
- E101 (= E211) binding
- C147 (= C262) modified: Disulfide link with 148
- C148 (≠ L270) modified: Disulfide link with 147
- R153 (≠ T275) binding
- T198 (= T354) binding
- GA 214:215 (≠ LN 370:371) binding
- E216 (≠ D372) binding
- T274 (≠ N421) binding
- N294 (= N441) binding
- D339 (= D485) binding
- K366 (= K513) binding
- NW 425:426 (= NW 611:612) binding
Sites not aligning to the query:
- 1:31 signal peptide
- 575 binding
- 635 binding covalent
- 638 binding covalent
- 639 binding axial binding residue
- 678 binding axial binding residue
1kb0A Crystal structure of quinohemoprotein alcohol dehydrogenase from comamonas testosteroni (see paper)
26% identity, 77% coverage: 167:789/814 of query aligns to 28:540/670 of 1kb0A
- active site: E185 (≠ T375), N263 (= N441), D308 (= D485)
- binding calcium ion: E185 (≠ T375), N263 (= N441), D308 (= D485)
- binding pyrroloquinoline quinone: E70 (= E211), C116 (= C262), C117 (≠ L270), R122 (≠ T275), T167 (= T354), G182 (≠ D372), G183 (≠ N373), A184 (≠ L374), E185 (≠ T375), T243 (≠ N421), W245 (= W423), D308 (= D485), K335 (= K513), N394 (= N611), W395 (= W612), W479 (≠ T726)
- binding tetrahydrofuran-2-carboxylic acid: C116 (= C262), C117 (≠ L270), E185 (≠ T375), D308 (= D485), P389 (≠ Y606)
Sites not aligning to the query:
- binding heme c: 598, 599, 602, 603, 617, 620, 631, 637, 640, 642, 643, 645
- binding pyrroloquinoline quinone: 543, 544
1lrwA Crystal structure of methanol dehydrogenase from p. Denitrificans (see paper)
25% identity, 77% coverage: 167:789/814 of query aligns to 13:536/600 of 1lrwA
- active site: E177 (= E378), N261 (= N441), D303 (= D485)
- binding calcium ion: E177 (= E378), N261 (= N441), D303 (= D485)
- binding pyrroloquinoline quinone: E55 (≠ Q213), C103 (≠ D269), C104 (≠ L270), R109 (≠ T275), T159 (= T354), S174 (≠ T375), G175 (≠ V376), A176 (≠ G377), E177 (= E378), T241 (≠ N421), W243 (= W423), R331 (≠ K513), W476 (≠ M727)
Sites not aligning to the query:
P12293 Methanol dehydrogenase [cytochrome c] subunit 1; MDH large subunit alpha; MEDH; EC 1.1.2.7 from Paracoccus denitrificans (see 2 papers)
24% identity, 77% coverage: 167:789/814 of query aligns to 45:567/631 of P12293
- C135 (≠ D269) modified: Disulfide link with 136
- C136 (≠ L270) modified: Disulfide link with 135
- C418 (≠ Y603) modified: Disulfide link with 447
- C447 (≠ R630) modified: Disulfide link with 418
Sites not aligning to the query:
2d0vA Crystal structure of methanol dehydrogenase from hyphomicrobium denitrificans (see paper)
25% identity, 77% coverage: 164:789/814 of query aligns to 10:536/597 of 2d0vA
- active site: E177 (= E378), N261 (= N441), D303 (= D485)
- binding calcium ion: E177 (= E378), N261 (= N441), D303 (= D485)
- binding pyrroloquinoline quinone: E55 (≠ Q213), R109 (≠ L273), T159 (= T354), S174 (≠ T375), G175 (≠ V376), A176 (≠ G377), E177 (= E378), T241 (≠ N421), W243 (= W423), R331 (≠ K513), N394 (= N611), W476 (≠ M727)
Sites not aligning to the query:
6oc6A Lanthanide-dependent methanol dehydrogenase xoxf from methylobacterium extorquens, in complex with lanthanum and pyrroloquinoline quinone (see paper)
25% identity, 75% coverage: 179:789/814 of query aligns to 25:533/579 of 6oc6A
- active site: E171 (= E378), N255 (= N441), D297 (= D485)
- binding pyrroloquinoline quinone: E55 (≠ Q213), C103 (≠ S253), C104 (≠ E254), R109 (= R263), T153 (≠ V353), S168 (≠ T375), G169 (≠ V376), G170 (= G377), E171 (= E378), W237 (= W423), D299 (= D487), R324 (≠ K513), D395 (≠ N611), W473 (≠ M727)
Sites not aligning to the query:
7ce5A Methanol-pqq bound methanol dehydrogenase (mdh) from methylococcus capsulatus (bath) (see paper)
24% identity, 77% coverage: 167:789/814 of query aligns to 13:529/573 of 7ce5A
- active site: E177 (≠ P379), N261 (= N441), D303 (= D485)
- binding calcium ion: E177 (≠ P379), N261 (= N441), D303 (= D485)
- binding methanol: E177 (≠ P379)
- binding pyrroloquinoline quinone: E55 (≠ Q213), C103 (≠ Y267), C104 (≠ A268), R109 (≠ L273), T159 (= T354), A174 (≠ V376), G175 (= G377), A176 (≠ E378), E177 (≠ P379), T241 (≠ N421), W243 (= W423), R330 (≠ K513), N393 (= N611), W469 (≠ M727)
Sites not aligning to the query:
7cdlC Holo-methanol dehydrogenase (mdh) with cys131-cys132 reduced from methylococcus capsulatus (bath) (see paper)
24% identity, 77% coverage: 167:789/814 of query aligns to 13:529/573 of 7cdlC
- active site: E177 (≠ P379), N261 (= N441), D303 (= D485)
- binding calcium ion: E177 (≠ P379), N261 (= N441), D303 (= D485)
- binding pyrroloquinoline quinone: E55 (≠ Q213), R109 (≠ L273), T159 (= T354), A174 (≠ V376), A176 (≠ E378), E177 (≠ P379), T241 (≠ N421), W243 (= W423), D303 (= D485), R330 (≠ K513), N393 (= N611), W469 (≠ M727)
Sites not aligning to the query:
6fkwA Europium-containing methanol dehydrogenase (see paper)
28% identity, 45% coverage: 168:535/814 of query aligns to 14:353/576 of 6fkwA
- active site: E172 (= E378), N256 (≠ T443), D299 (= D485), D301 (= D487)
- binding europium ion: E172 (= E378), N256 (≠ T443), D299 (= D485), D301 (= D487)
- binding pyrroloquinoline quinone: E55 (≠ Q213), C104 (≠ D269), C105 (≠ L270), R110 (≠ T275), T154 (= T354), S169 (≠ T375), G170 (≠ V376), G171 (= G377), E172 (= E378), T236 (≠ N421), W238 (= W423), D301 (= D487), R326 (≠ K513)
Sites not aligning to the query:
4maeA Methanol dehydrogenase from methylacidiphilum fumariolicum solv (see paper)
28% identity, 45% coverage: 168:535/814 of query aligns to 14:353/577 of 4maeA
- active site: E172 (= E378), N256 (≠ T443), D299 (= D485)
- binding cerium (iii) ion: E172 (= E378), N256 (≠ T443), D299 (= D485), D301 (= D487)
- binding pyrroloquinoline quinone: E55 (= E211), C104 (≠ D269), C105 (≠ L270), R110 (≠ T275), T154 (= T354), S169 (≠ T375), G170 (≠ V376), G171 (= G377), E172 (= E378), T236 (≠ N421), W238 (= W423), D301 (= D487), R326 (≠ K513)
Sites not aligning to the query:
1w6sC The high resolution structure of methanol dehydrogenase from methylobacterium extorquens (see paper)
23% identity, 77% coverage: 166:789/814 of query aligns to 12:536/596 of 1w6sC
- active site: E177 (= E378), N261 (= N441), D303 (= D485)
- binding calcium ion: E177 (= E378), N261 (= N441)
- binding pyrroloquinoline quinone: E55 (≠ Q213), C103 (≠ Y267), C104 (≠ A268), R109 (≠ L273), T159 (= T354), S174 (≠ T375), A176 (≠ G377), E177 (= E378), T241 (≠ N421), W243 (= W423), R331 (≠ K513), N394 (≠ G589), W476 (≠ M727)
Sites not aligning to the query:
Query Sequence
>WP_057507612.1 NCBI__GCF_001431535.1:WP_057507612.1
MQSVSSKLAQGAGILFALLGVLLAAGGVWLITLGGSWFYLLAGAVLAATGVALVRLRASA
LWMALGLLLFAAVWALWEVGLDFWQLVPRLIAFLVVAMVTAAFAPRLHRRDLAPALRCRP
AGALAGVLALGLVGFFAGMFVPHPTVEADGSRAKVTNAAAGQAAGNDWPAYGRTSSGDRY
AQFDQINTTNVKDLQVAWTYRTGDLAIDGAEYQVTPLKIDDTVYLCTPLSKVVAVDATTG
KEKWRFDPKAVVSESTQGWKRCRGVGYADLDALDTATAASTATAPAQAVAANTPAAPAIC
RKRIIETTIDARLLALDAETGKLCEDFGDKGYVDLGIGIAPAPKDSDAGTYNVTSAPLVA
DGVVMVGGRLNDNLTVGEPGGVVRGYDVVTGKILWAWDAKRGATDSTPLPEGQLYPLETP
NFWGTAAYDPKLGLAYFPLGNQTPDFWTGNRHDYSDEYTDAVVAVELKTGKERWHFRTAN
IDQFDYDVSSQPILYDMPGKNGETTPVLVQLTKRGQVFVLDRRTGQPVVPVEYRKVSTDA
MPGMKVAPTQPHSAIAVGVEPFKESDMWGASIFDQLYCRIQFKQMRWEGEFTPLSDKQRT
LIYPGYYGGFNWGGGAVDASTGTLIVNDIRMAQWGRFIKQEEAKRIGYKPSTEGEYSTQT
GTPWGVERSMFVSPLGVPCFKPPFGTMTAIDLSTGKTRWQVPMGTIKDAPVHGIAPGVDI
PLGMPTMGGPLVTKGGLTFFHGSLDYYVRALDNDTGKVLWQGRLPVGGQGAPMSYVGKDG
RQYVVVVAGGATRTGTNDNRGDYVIAYALPKAGK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory