SitesBLAST
Comparing WP_057507751.1 NCBI__GCF_001431535.1:WP_057507751.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q9A3Q9 Omega-aminotransferase; Beta-alanine--pyruvate aminotransferase; EC 2.6.1.-; EC 2.6.1.18 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see paper)
59% identity, 95% coverage: 17:442/450 of query aligns to 7:431/439 of Q9A3Q9
- V227 (= V237) mutation to G: Decreases activity toward 3-aminobutanoate by 2-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 2-fold.
- R260 (= R270) mutation to L: Decreases activity toward 3-aminobutanoate by 30-fold.
- N285 (= N295) mutation to A: Decreases activity toward 3-aminobutanoate by 4-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 3-fold.
Q9I700 Beta-alanine--pyruvate aminotransferase; Beta-A--Py AT; Beta-alanine--pyruvate transaminase; Omega-amino acid aminotransferase; Omega-amino acid AT; Omega-amino acid--pyruvate aminotransferase; Omega-APT; EC 2.6.1.18 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
49% identity, 99% coverage: 7:450/450 of query aligns to 4:448/448 of Q9I700
- W61 (= W63) binding substrate
- T327 (= T328) binding pyridoxal 5'-phosphate
- R414 (= R416) binding substrate
- Q421 (≠ A423) binding substrate
4b98A The structure of the omega aminotransferase from pseudomonas aeruginosa (see paper)
49% identity, 96% coverage: 18:450/450 of query aligns to 9:441/441 of 4b98A
- active site: F17 (= F26), Y146 (= Y155), E219 (= E229), D252 (= D262), I255 (= I265), K281 (= K291), Q414 (≠ A423)
- binding pyridoxal-5'-phosphate: G233 (= G243), Q236 (= Q246), F270 (= F280), G271 (= G281)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: L53 (= L62), W54 (= W63), F82 (= F91), S112 (= S121), G113 (= G122), S114 (= S123), Y146 (= Y155), H147 (= H156), G148 (= G157), E219 (= E229), S224 (= S234), D252 (= D262), V254 (= V264), I255 (= I265), K281 (= K291), Y319 (= Y327), T320 (= T328), R407 (= R416), Q414 (≠ A423)
4uhmA Characterization of a novel transaminase from pseudomonas sp. Strain aac (see paper)
48% identity, 96% coverage: 18:450/450 of query aligns to 3:435/435 of 4uhmA
- active site: F11 (= F26), Y140 (= Y155), E213 (= E229), D246 (= D262), I249 (= I265), K275 (= K291), Q408 (≠ A423)
- binding magnesium ion: A91 (= A106), D99 (≠ N114)
- binding pyridoxal-5'-phosphate: G107 (= G122), S108 (= S123), Y140 (= Y155), H141 (= H156), G142 (= G157), E213 (= E229), D246 (= D262), V248 (= V264), I249 (= I265), K275 (= K291)
3a8uX Crystal structure of omega-amino acid:pyruvate aminotransferase
49% identity, 95% coverage: 19:447/450 of query aligns to 9:438/441 of 3a8uX
- active site: Y145 (= Y155), D252 (= D262), K281 (= K291), Q414 (≠ A423)
- binding pyridoxal-5'-phosphate: S111 (= S121), G112 (= G122), S113 (= S123), Y145 (= Y155), H146 (= H156), G147 (= G157), E219 (= E229), D252 (= D262), V254 (= V264), I255 (= I265), K281 (= K291)
6fyqA The crystal structure of a new transaminase from the marine bacterium virgibacillus (see paper)
37% identity, 94% coverage: 22:442/450 of query aligns to 11:431/443 of 6fyqA
- active site: F15 (= F26), Y147 (= Y155), D243 (= D262), K272 (= K291)
- binding pyridoxal-5'-phosphate: G114 (= G122), S115 (= S123), Y147 (= Y155), H148 (= H156), G149 (= G157), E210 (= E229), D243 (= D262), V245 (= V264), I246 (= I265), K272 (= K291)
7ypmA Crystal structure of transaminase cc1012 complexed with plp and l- alanine (see paper)
39% identity, 91% coverage: 33:442/450 of query aligns to 27:440/454 of 7ypmA
- binding alanine: W57 (= W63), Y150 (= Y155)
- binding pyridoxal-5'-phosphate: S116 (= S121), G117 (= G122), S118 (= S123), Y150 (= Y155), G152 (= G157), E223 (= E229), D256 (= D262), V258 (= V264), I259 (= I265), K285 (= K291)
3i5tA Crystal structure of aminotransferase prk07036 from rhodobacter sphaeroides kd131
38% identity, 91% coverage: 33:442/450 of query aligns to 22:430/444 of 3i5tA
- active site: Y144 (= Y155), E216 (= E229), D249 (= D262), V252 (≠ I265), K279 (= K291), V411 (≠ A423)
- binding pyridoxal-5'-phosphate: G111 (= G122), S112 (= S123), Y144 (= Y155), H145 (= H156), E216 (= E229), D249 (= D262), V251 (= V264), K279 (= K291), Y315 (= Y327), T316 (= T328)
Sites not aligning to the query:
7ypnD Crystal structure of transaminase cc1012 mutant m9 complexed with plp (see paper)
38% identity, 91% coverage: 33:442/450 of query aligns to 27:440/455 of 7ypnD
- binding pyridoxal-5'-phosphate: S116 (= S121), G117 (= G122), S118 (= S123), Y150 (= Y155), H151 (= H156), G152 (= G157), E223 (= E229), D256 (= D262), V258 (= V264), K285 (= K291), F318 (≠ Y327), T319 (= T328)
6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
38% identity, 94% coverage: 25:446/450 of query aligns to 19:443/448 of 6io1B
- active site: L20 (≠ F26), Y151 (= Y155), D257 (= D262), K286 (= K291)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (≠ S121), G118 (= G122), A119 (≠ S123), N122 (≠ V126), Y151 (= Y155), H152 (= H156), D257 (= D262), V259 (= V264), I260 (= I265), K286 (= K291)
6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
38% identity, 95% coverage: 25:450/450 of query aligns to 17:446/450 of 6gwiB