SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_057507901.1 NCBI__GCF_001431535.1:WP_057507901.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7ocrB NADPH and fructose-6-phosphate bound to the dehydrogenase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
32% identity, 90% coverage: 15:217/226 of query aligns to 13:211/675 of 7ocrB

query
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7ocrB

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binding magnesium ion: D15 (= D17), D17 (= D19), D169 (= D175)

Sites not aligning to the query:

binding fructose -6-phosphate: 362, 403, 508, 513, 516, 587, 590, 594, 595, 601
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 244, 246, 247, 248, 273, 274, 329, 330, 333, 361, 362, 406, 587, 590

7ocqB Nadh bound to the dehydrogenase domain of the bifunctional mannitol-1- phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
30% identity, 89% coverage: 15:216/226 of query aligns to 13:211/679 of 7ocqB

query
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7ocqB

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binding magnesium ion: D15 (= D17), D17 (= D19), D170 (= D175)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 245, 246, 247, 248, 249, 274, 275, 329, 330, 334, 363, 403, 405, 407, 591, 594

7ocqA Nadh bound to the dehydrogenase domain of the bifunctional mannitol-1- phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
29% identity, 89% coverage: 15:216/226 of query aligns to 13:216/686 of 7ocqA

query
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7ocqA

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binding magnesium ion: D15 (= D17), D17 (= D19), D175 (= D175)

Sites not aligning to the query:

binding [[(2~{R},3~{S},4~{R},5~{R})-5-(3-aminocarbonyl-4~{H}-pyridin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{R},5~{R})-3,4,5-tris(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: 252, 253, 254, 279, 280, 335, 369, 370

7ocnA Crystal structure of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
29% identity, 89% coverage: 15:216/226 of query aligns to 13:216/690 of 7ocnA

query
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7ocnA

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binding magnesium ion: D15 (= D17), D17 (= D19), D175 (= D175)

7ocpA NADPH bound to the dehydrogenase domain of the bifunctional mannitol- 1-phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
29% identity, 89% coverage: 15:216/226 of query aligns to 13:216/688 of 7ocpA

query
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7ocpA

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binding magnesium ion: D15 (= D17), D17 (= D19), D175 (= D175)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 250, 251, 253, 254, 279, 280, 334, 335, 336, 339, 369, 370

7ocsC Mannitol-1-phosphate bound to the phosphatase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld-d16a from acinetobacter baumannii (see paper)
30% identity, 89% coverage: 15:216/226 of query aligns to 13:216/572 of 7ocsC

query
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7ocsC

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S

R

Q

L

L

G

H

K

L

D

D

P

A

A

N

R

Q

C

C

L

L

A

M

L

F

E

E

D

D

S

S

P

E

T

N

G

G

-

L

T

T

R

S

A

A

A

H

L

T

A

S

A

K

G

G

M

L

T

T

A

-

I

I

Q

L

V

L

P

K

D

D

L

I

L

K

H

E

P

P

D

N

A

D

A

E

V

M

R

L

A

E

L

K

G

A

H

H

R

F

I

Y

V

Y

D

D

S

Q

L

M

H

Y

D

D

binding D-Mannitol-1-phosphate: M16 (= M18), D17 (= D19), E24 (= E26), R27 (≠ S29), L31 (≠ W33), C51 (≠ M52), L52 (≠ V53), G53 (= G54), L54 (= L55), R77 (= R71), T117 (= T117), S118 (= S118), K150 (= K150)

7ocsA Mannitol-1-phosphate bound to the phosphatase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld-d16a from acinetobacter baumannii (see paper)
28% identity, 89% coverage: 15:216/226 of query aligns to 13:216/682 of 7ocsA

query
sites
7ocsA

I

I

F

F

D

A

M
|
M

D
|
D

G

G

L

T

M

M

I

F

D

D

S

T

E
|
E

R

R

V

L

S
x
R

I

F

A

Q

C

T

W
x
L

S

Q

E

Q

A

A

S

S

V

Q

A

E

L

L

-

I

G

G

I

Q

A

E

L

F

P

S

E

H

G

E

F

Y

F

L

L

M

H

Q

M
x
C

V
x
L

G

G

L
|
L

G

S

E

A

R

T

D

T

C

A

L

E

E

K

L

L

L

A

Q

Q

R

R

H

L

V

Y

D

G

D

V

R

D

A

V

R

P

I

Y

N

K

A

E

L

I

L

R

A

K

R

R

C

A

H

D

A

E

L

M

-

E

Y

L

D

E

A

H

R

I

T

R

Q

K

D

H

G

G

L

V

P

P

L

I

R

K

P

K

G

G

I

L

L

V

E

Q

L

V

L

L

E

E

L

R

L

L

K

R

Q

K

H

S

E

G

V

L

P

R

R

M

A

A

V

V

A

A

T
|
T

S
|
S

T

S

R

R

Q

R

P

A

R

I

A

A

S

E

R

E

K

Y

L

L

A

I

A

N

S

A

G

N

L

V

L

Y

D

K

Y

F

F

F

D

D

A

V

V

I

V

T

T

C

S

G

S

D

D

E

V

V

Q

E

H

Q

P

G

K
|
K

P

P

A

H

P

P

D

E

I

I

Y

F

L

L

L

K

A

A

R

S

R

Q

L

L

G

H

K

L

D

D

P

A

A

N

R

Q

C

C

L

L

A

M

L

F

E

E

D

D

S

S

P

E

T

N

G

G

T

L

R

T

A

S

A

A

L

H

A

T

A

S

G

K

M

G

T

L

A

T

I

I

Q

L

V

L

P

K

D

D

L

I

L

K

H

E

P

P

D

N

A

D

A

E

V

M

R

L

A

E

L

K

G

A

H

H

R

F

I

Y

V

Y

D

D

S

Q

L

M

H

Y

D

D

binding D-Mannitol-1-phosphate: M16 (= M18), D17 (= D19), E24 (= E26), R27 (≠ S29), L31 (≠ W33), C51 (≠ M52), L52 (≠ V53), L54 (= L55), T117 (= T117), S118 (= S118), K150 (= K150)

Sites not aligning to the query:

binding magnesium ion: 372, 411

Q8VZ10 Protein SUPPRESSOR OF QUENCHING 1, chloroplastic; EC 3.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 82% coverage: 13:197/226 of query aligns to 76:264/1055 of Q8VZ10

query
sites
Q8VZ10

A

A

V

V

I

L

F

F

D
|
D

M

M

D

D

G

G

L

V

M

L

I

C

D

N

S

S

E

E

R

D

V

L

S

S

I

R

A

R

C

A

W

A

S

V

E

D

A

V

S

F

V

T

A

E

L

M

G

G

I

V

A

E

L

V

P

T

E

V

G

D

F

D

F

F

L

V

H

P

M

F

V

M

G

G

L

T

G

G

E

E

R

A

D

K

C

F

L

L

E

G

L

G

L

V

Q

A

R

S

H

V

V

K

D

E

D

V

R

K

A

G

-

F

R

D

I

P

N

D

A

A

L

A

L

K

A

E

R

R

C

F

H

F

A

E

L

I

Y

Y

-

L

-

D

-

K

D

Y

A

A

R

K

T

P

Q

E

D

S

G

G

L

I

P

G

L

F

R

-

P

P

G

G

I

A

L

L

E

E

L

L

L

V

E

T

L

E

L

C

K

K

Q

N

H

K

E

G

V

L

P

K

R

V

A

A

V

V

A

A

T

S

S

S

T

A

R

D

Q

R

P

I

R

K

A

V

S

D

R

A

K

N

L

L

A

K

A

A

S

A

G

G

L

L

-

S

L

L

D

T

Y

M

F

F

D

D

A

A

V

I

V

V

T

S

S

A

S

D

D

A

V

F

Q

E

H

N

P

L

K

K

P

P

A

A

P

P

D

D

I

I

Y

F

L

L

L

A

A

A

R

K

R

I

L

L

G

G

K

V

D

P

P

T

A

S

R

E

C

C

L

V

A

V

L

I

E

E

D

D

S

A

P

L

T

A

G

G

T

V

R

Q

A

A

L

Q

A

A

G

N

M

M

T

R

A

C

I

I

Q

A

V

V

P

K

D

T

L

T

L

L

D80 (= D17) mutation to N: Complete rescue in complementation test of the nonphotochemical quenching (NPQ) phenotype observed in disrupted plants.

Sites not aligning to the query:

431:434 CINC→SINS: No rescue in complementation test of the nonphotochemical quenching (NPQ) phenotype observed in disrupted plants.
859 E→K: In soq1-2; high light intensity-dependent and irreversible nonphotochemical quenching (NPQ) due to a decrease in chlorophyll excited-state lifetime.

P71447 Beta-phosphoglucomutase; Beta-PGM; EC 5.4.2.6 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see 3 papers)
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/221 of P71447

query
sites
P71447

A

A

V

V

I

L

F

F

D
|
D

M

L

D
|
D

G

G

L

V

M

I

I

T

D

D

S
x
T

E

A

R

E

V

Y

S
x
H

I

F

A

R

C

A

W

W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M

L

V
x
K

G
|
G

L

V

G

S

E
x
R

R

E

D

D

C
x
S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C
x
K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T

S

S

A

T

S

R

K

Q

N

-

G

P

P

R

F

A

L

S

L

R

E

K

K

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K

K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E

E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

D8 (= D17) modified: 4-aspartylphosphate; mutation to A: Inactive.; mutation to E: Inactive.
D10 (= D19) mutation to A: Inactive.; mutation to E: Inactive.; mutation to N: Inactive.; mutation to S: Inactive.
T16 (≠ S25) mutation to P: 500-fold reduction in the rate constant for Asp-8 phosphorylation by beta-G1,6bisP. 6,700-fold reduction in the apparent rate constant for cycling of the phosphorylated enzyme to convert beta-G1P to G6P. 13-fold increase in the estimated rate constant for phosphoryl transfer from the phospho-Asp8 to water.
H20 (≠ S29) mutation to A: Impairs Asp-8 phosphorylation by beta-G1,6bisP and phosphoryl transfer from the phospho-Asp8 to the substrate beta-G1P.; mutation to N: 300-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.; mutation to Q: 8-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
K45 (≠ V53) mutation to A: 20'000-fold decrease in kcat/KM.
G46 (= G54) mutation to A: 1'000'000-fold decrease in kcat/KM.; mutation to P: 100'000-fold decrease in kcat/KM.; mutation to V: 10'000-fold decrease in kcat/KM.
R49 (≠ E57) mutation to K: 1'000'000-fold decrease in kcat/KM.
S52 (≠ C60) mutation to A: Wild-type activity.
K76 (≠ C81) mutation to A: 100-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
D170 (= D175) mutation to A: Impaired, but active with an increase in the affinity for G1P.

6h91A Phosphorylated beta-phosphoglucomutase from lactococcus lactis in an open conformer to 2.4 a
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/218 of 6h91A

query
sites
6h91A

A

A

V

V

I

L

F

F

D
|
D

M

L

D
|
D

G

G

L

V

M

I

I

T

D

D

S

T

E

A

R

E

V

Y

S

H

I

F

A

R

C

A

W

W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M

L

V

K

G

G

L

V

G

S

E
x
R

R

E

D

D

C

S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T

S

S

A

T

S

R
x
K

Q

N

-

G

P

P

R

F

A

L

S

L

R

E

K

R

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K

K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E

E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

binding magnesium ion: D8 (= D17), D10 (= D19), D170 (= D175)
binding acetylphosphate: R49 (≠ E57), K117 (≠ R120)

4c4rA Structure of beta-phosphoglucomutase in complex with a phosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride (see paper)
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/218 of 4c4rA

query
sites
4c4rA

A

A

V

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

L

V

M

I

I

T

D

D

S
x
T

E

A

R

E

V

Y

S
x
H

I

F

A

R

C

A

W
|
W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M
x
L

V
x
K

G
|
G

L
x
V

G

S

E
x
R

R

E

D

D

C
x
S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T
x
S

S
x
A

T
x
S

R
x
K

Q

N

-

G

P

P

R

F

A

L

S

L

R

E

K

R

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K
|
K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E
|
E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

active site: D8 (= D17), L9 (≠ M18), D10 (= D19), T16 (≠ S25), K45 (≠ V53), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding magnesium ion: D8 (= D17), D10 (= D19), D170 (= D175)
binding trifluoromagnesate: D8 (= D17), L9 (≠ M18), D10 (= D19), S114 (≠ T117), A115 (≠ S118), K145 (= K150)
binding (1R)-1,5-anhydro-1-(phosphonomethyl)-D-glucitol: D10 (= D19), H20 (≠ S29), W24 (= W33), L44 (≠ M52), G46 (= G54), V47 (≠ L55), R49 (≠ E57), S52 (≠ C60), S116 (≠ T119), K117 (≠ R120)

3zi4A The structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and scandium tetrafluoride
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/218 of 3zi4A

query
sites
3zi4A

A

A

V

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

L

V

M

I

I

T

D

D

S
x
T

E

A

R

E

V

Y

S
x
H

I

F

A

R

C

A

W

W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M

L

V
x
K

G
|
G

L
x
V

G

S

E
x
R

R

E

D

D

C

S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T
x
S

S
x
A

T
x
S

R
x
K

Q

N

-

G

P

P

R

F

A

L

S

L

R

E

K

R

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K
|
K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E
|
E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

active site: D8 (= D17), L9 (≠ M18), D10 (= D19), T16 (≠ S25), K45 (≠ V53), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D19), H20 (≠ S29), G46 (= G54), V47 (≠ L55), R49 (≠ E57), S116 (≠ T119), K117 (≠ R120)
binding magnesium ion: D8 (= D17), D10 (= D19), D170 (= D175)
binding Scandium Tetrafluoride: D8 (= D17), L9 (≠ M18), D10 (= D19), S114 (≠ T117), A115 (≠ S118), K145 (= K150)

2wf8A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate, glucose-1-phosphate and beryllium trifluoride (see paper)
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/218 of 2wf8A

query
sites
2wf8A

A

A

V

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

L

V

M

I

I

T

D

D

S
x
T

E

A

R

E

V

Y

S
x
H

I

F

A

R

C

A

W
|
W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M
x
L

V
x
K

G
|
G

L
x
V

G

S

E
x
R

R

E

D

D

C
x
S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T
x
S

S
x
A

T
x
S

R
x
K

Q

N

-

G

P

P

R

F

A

L

S

L

R

E

K

R

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K
|
K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E
|
E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

active site: D8 (= D17), L9 (≠ M18), D10 (= D19), T16 (≠ S25), K45 (≠ V53), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding beryllium trifluoride ion: D8 (= D17), L9 (≠ M18), D10 (= D19), S114 (≠ T117), A115 (≠ S118), K145 (= K150)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D19), H20 (≠ S29), G46 (= G54), V47 (≠ L55), R49 (≠ E57), A115 (≠ S118), S116 (≠ T119), K117 (≠ R120)
binding 1-O-phosphono-alpha-D-glucopyranose: D10 (= D19), H20 (≠ S29), W24 (= W33), L44 (≠ M52), G46 (= G54), V47 (≠ L55), R49 (≠ E57), S52 (≠ C60), A115 (≠ S118), S116 (≠ T119), K117 (≠ R120)
binding magnesium ion: D8 (= D17), D10 (= D19), D170 (= D175)

2wf7A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphonate and aluminium tetrafluoride (see paper)
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/218 of 2wf7A

query
sites
2wf7A

A

A

V

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

L

V

M

I

I

T

D

D

S
x
T

E

A

R

E

V

Y

S

H

I

F

A

R

C

A

W

W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M

L

V
x
K

G
|
G

L
x
V

G

S

E
x
R

R

E

D

D

C

S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T
x
S

S
x
A

T
x
S

R
x
K

Q
x
N

-

G

P

P

R

F

A

L

S

L

R

E

K

R

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K
|
K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E
|
E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

active site: D8 (= D17), L9 (≠ M18), D10 (= D19), T16 (≠ S25), K45 (≠ V53), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding tetrafluoroaluminate ion: D8 (= D17), L9 (≠ M18), D10 (= D19), S114 (≠ T117), K145 (= K150)
binding 6,7-dideoxy-7-phosphono-beta-D-gluco-heptopyranose: D10 (= D19), G46 (= G54), V47 (≠ L55), R49 (≠ E57), S116 (≠ T119), K117 (≠ R120), N118 (≠ Q121)
binding magnesium ion: D8 (= D17), D10 (= D19), D170 (= D175)

2wf6A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and aluminium tetrafluoride (see paper)
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/218 of 2wf6A

query
sites
2wf6A

A

A

V

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

L

V

M

I

I

T

D

D

S
x
T

E

A

R

E

V

Y

S

H

I

F

A

R

C

A

W

W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M

L

V
x
K

G
|
G

L
x
V

G

S

E
x
R

R

E

D

D

C

S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T
x
S

S
x
A

T
x
S

R
x
K

Q

N

-

G

P

P

R

F

A

L

S

L

R

E

K

R

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K
|
K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E
|
E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

active site: D8 (= D17), L9 (≠ M18), D10 (= D19), T16 (≠ S25), K45 (≠ V53), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding tetrafluoroaluminate ion: D8 (= D17), L9 (≠ M18), D10 (= D19), S114 (≠ T117), K145 (= K150)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D19), G46 (= G54), V47 (≠ L55), R49 (≠ E57), S116 (≠ T119), K117 (≠ R120)
binding magnesium ion: D8 (= D17), D10 (= D19), D170 (= D175)

2wf5A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and trifluoromagnesate (see paper)
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/218 of 2wf5A

query
sites
2wf5A

A

A

V

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

L

V

M

I

I

T

D

D

S
x
T

E

A

R

E

V

Y

S
x
H

I

F

A

R

C

A

W

W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M

L

V
x
K

G
|
G

L
x
V

G

S

E
x
R

R

E

D

D

C

S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T
x
S

S
x
A

T
x
S

R

K

Q

N

-

G

P

P

R

F

A

L

S

L

R

E

K

R

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K
|
K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E
|
E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

active site: D8 (= D17), L9 (≠ M18), D10 (= D19), T16 (≠ S25), K45 (≠ V53), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D19), H20 (≠ S29), G46 (= G54), V47 (≠ L55), R49 (≠ E57), A115 (≠ S118), S116 (≠ T119)
binding magnesium ion: D8 (= D17), D10 (= D19), D170 (= D175)
binding trifluoromagnesate: D8 (= D17), L9 (≠ M18), D10 (= D19), S114 (≠ T117), A115 (≠ S118), K145 (= K150)

6qzgA Beta-glucose 1,6-bisphosphonate bound to wild type beta- phosphoglucomutse in an open conformation.
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/219 of 6qzgA

query
sites
6qzgA

A

A

V

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

L

V

M

I

I

T

D

D

S

T

E

A

R

E

V

Y

S
x
H

I

F

A

R

C

A

W

W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M

L

V

K

G
|
G

L

V

G

S

E

R

R

E

D

D

C

S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T
x
S

S
x
A

T
x
S

R
x
K

Q

N

-

G

P

P

R

F

A

L

S

L

R

E

K

R

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K
|
K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E

E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

binding 3,7-anhydro-1,2,8-trideoxy-1,8-diphosphono-D-glycero-D-gulo-octitol: D8 (= D17), L9 (≠ M18), D10 (= D19), H20 (≠ S29), G46 (= G54), S114 (≠ T117), A115 (≠ S118), S116 (≠ T119), K117 (≠ R120), K145 (= K150)
binding magnesium ion: D8 (= D17), D10 (= D19), D170 (= D175)

1z4nA Structure of beta-phosphoglucomutase with inhibitor bound alpha- galactose 1-phosphate cocrystallized with fluoride (see paper)
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/219 of 1z4nA

query
sites
1z4nA

A

A

V

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

L

V

M

I

I

T

D

D

S
x
T

E

A

R

E

V

Y

S
x
H

I

F

A

R

C

A

W
|
W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M

L

V
x
K

G

G

L
x
V

G

S

E
x
R

R

E

D

D

C

S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T
x
S

S
x
A

T
x
S

R
x
K

Q
x
N

-

G

P

P

R

F

A

L

S

L

R

E

K

R

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K
|
K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E
|
E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

active site: D8 (= D17), L9 (≠ M18), D10 (= D19), T16 (≠ S25), K45 (≠ V53), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding 1-O-phosphono-alpha-D-galactopyranose: H20 (≠ S29), W24 (= W33), V47 (≠ L55), R49 (≠ E57), S116 (≠ T119), K117 (≠ R120), N118 (≠ Q121)
binding magnesium ion: D8 (= D17), D10 (= D19), E169 (= E174), D170 (= D175)

5olwA 5-fluorotryptophan labeled beta-phosphoglucomutase in an open conformation (see paper)
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/224 of 5olwA

query
sites
5olwA

A

A

V

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

L

V

M

I

I

T

D

D

S
x
T

E

A

R

E

V

Y

S

H

I

F

A

R

C

A

W

W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M

L

V
x
K

G

G

L

V

G

S

E

R

R

E

D

D

C

S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P
|
P

-

A

-

D

L
x
V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T
x
S

S
x
A

T

S

R

K

Q

N

-

G

P

P

R

F

A

L

S

L

R
x
E

K

R

L

M

A
x
N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K
|
K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E
|
E

D
|
D

S
|
S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

active site: D8 (= D17), L9 (≠ M18), D10 (= D19), T16 (≠ S25), K45 (≠ V53), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding calcium ion: D8 (= D17), D10 (= D19), P89 (= P94), V92 (≠ L95), E124 (≠ R126), N127 (≠ A129), E169 (= E174), D170 (= D175), S171 (= S176)

2wf9A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate, and beryllium trifluoride, crystal form 2 (see paper)
30% identity, 80% coverage: 13:193/226 of query aligns to 4:188/221 of 2wf9A

query
sites
2wf9A

A

A

V

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

L

V

M

I

I

T

D

D

S
x
T

E

A

R

E

V

Y

S
x
H

I

F

A

R

C

A

W

W

S

K

E

A

A

L

S

A

V

E

A

E

L

I

G

G

I

I

-

N

A

G

L

V

P

D

E

R

G

Q

F

F

F

N

L

E

H

Q

M

L

V
x
K

G
|
G

L
x
V

G

S

E
x
R

R

E

D

D

C

S

L

L

E

Q

L

K

L

I

Q

L

R

D

H

L

V

A

D

D

D

K

R

K

A

V

-

S

-

A

-

E

R

E

I

F

N

K

A

E

L

L

L

A

A

K

R

R

C

K

H

N

A

D

L

N

Y

Y

D

V

A

K

R

M

T

I

Q

Q

D

D

G

V

L

S

P

P

-

A

-

D

L

V

R

Y

P

P

G

G

I

I

L

L

E

Q

L

L

E

K

L

D

L

L

K

R

Q

S

H

N

E

K

V

I

P

K

R

I

A

A

V

L

A

A

T
x
S

S
x
A

T
x
S

R
x
K

Q
x
N

-

G

P

P

R

F

A

L

S

L

R

E

K

R

L

M

A

N

A

L

S

T

G

G

L

-

D

-

Y

Y

F

F

D

D

A

A

V

I

V

A

T

D

S

P

S

A

D

E

V

V

Q

A

H

A

P

S

K
|
K

P

P

A

A

P

P

D

D

I

I

Y

F

L

I

L

A

A

A

R

H

R

A

L

V

G

G

K

V

D

A

P

P

A

S

R

E

C

S

L

I

A

G

L

L

E
|
E

D
|
D

S

S

P

Q

T

A

G

G

T

I

R

Q

A

A

A

I

L

K

A

D

A

S

G

G

M

A

T

L

A

P

I

I

Q

G

V

V

active site: D8 (= D17), L9 (≠ M18), D10 (= D19), T16 (≠ S25), K45 (≠ V53), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding beryllium trifluoride ion: D8 (= D17), L9 (≠ M18), D10 (= D19), S114 (≠ T117), A115 (≠ S118), K145 (= K150)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D19), H20 (≠ S29), G46 (= G54), V47 (≠ L55), R49 (≠ E57), S116 (≠ T119), K117 (≠ R120), N118 (≠ Q121)
binding 6-O-phosphono-alpha-D-glucopyranose: D10 (= D19), H20 (≠ S29), G46 (= G54), V47 (≠ L55), R49 (≠ E57), A115 (≠ S118), S116 (≠ T119), K117 (≠ R120), N118 (≠ Q121)
binding magnesium ion: D8 (= D17), D10 (= D19), D170 (= D175)

Query Sequence

>WP_057507901.1 NCBI__GCF_001431535.1:WP_057507901.1
MSTIPALPFRPDAVIFDMDGLMIDSERVSIACWSEASVALGIALPEGFFLHMVGLGERDC
LELLQRHVDDRARINALLARCHALYDARTQDGLPLRPGILELLELLKQHEVPRAVATSTR
QPRASRKLAASGLLDYFDAVVTSSDVQHPKPAPDIYLLAARRLGKDPARCLALEDSPTGT
RAALAAGMTAIQVPDLLHPDAAVRALGHRIVDSLHDVRALLVPMLG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory