SitesBLAST

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 77% coverage: 33:186/201 of query aligns to 41:195/203 of P07464

query
sites
P07464

P

P

N

S

E

E

R

V

L

E

R

K

A

R

M

E

Y

S

L

L

R

I

R

K

V

E

M

M

K

F

V

A

A

T

V

V

G

G

Q

E

D

N

T

A

H

W

V

V

S

E

M

P

R

P

-

V

W

Y

F

F

V
x
S

T

Y

G

G

Y

-

P

-

V

-

R

-

A

S

N

N

V

I

T

H

I

I

G

G

N

R

N

N

C

F

V

Y

I

A

N
|
N

R

F

E

N

V

L

Y

T

L

I

D

V

G

D

R
x
D

V

Y

G

T

V

V

H

T

I

I

G

G

N

D

N

N

V

V

-

L

-

I

-

A

-

P

N

N

V

V

S

T

F

L

Q

S

T

V

C

T

I

G

L
x
H

S

P

L

V

H

H

D

E

H

L

N

R

S

K

P

-

D

N

F

G

I

E

A

M

V

Y

G

S

S

F

A

P

V

I

T

T

I

I

Q

G

D

N

H

N

V

V

W

W

I

I

G

G

A
x
S

R

H

A

V

T

V

I

I

L

N

P

P

G

G

V

V

T

T

V

I

G

G

E

D

G

N

A

S

V

V

I

I

A

G

A
|
A

G

G

A

S

V

I

V

V

A
x
T

R
x
K

D

D

V

I

P

P

A

P

Y

N

D

V

V

V

V

A

G

A

G

G

V

V

P

P

A

C

K
x
R

K

V

I

I

G
x
R

E

E

R

I

N

N

-

D

R

R

D

D

I

K

R

H

Y

Y

L

Y

T

F

K

K

S71 (≠ V62) binding
N85 (= N80) binding in other chain; binding in other chain
D93 (≠ R88) binding
H115 (≠ L106) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A134) binding in other chain
A160 (= A152) binding in other chain
TK 165:166 (≠ AR 157:158) binding
R180 (≠ K172) binding
R183 (≠ G175) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
33% identity, 77% coverage: 33:186/201 of query aligns to 40:194/201 of 1krvA

query
sites
1krvA

P

P

N

S

E

E

R

V

L

E

R

K

A

R

M

E

Y

S

L

L

R

I

R

K

V

E

M

M

K

F

V

A

A

T

V

V

G

G

Q

E

D

N

T

A

H

W

V

V

S

E

M

P

R

P

-

V

W

Y

F

F

V
x
S

T

Y

G

G

Y

-

P

-

V

-

R

-

A

S

N

N

V

I

T

H

I

I

G

G

N

R

N

N

C

F

V
x
Y

I

A

N
|
N

R

F

E

N

V

L

Y

T

L

I

D
x
V

G

D

R
x
D

V

Y

G

T

V

V

H

T

I

I

G

G

N

D

N

N

V

V

-

L

-

I

-
x
A

-

P

N

N

V

V

S

T

F

L

Q
x
S

T

V

C
x
T

I

G

L

H

S

P

L

V

H

H

D

E

H

L

N

R

S

K

P

-

D

N

F

G

I

E

A
x
M

V

Y

G

S

S

F

A

P

V

I

T

T

I

I

Q

G

D

N

H

N

V

V

W
|
W

I

I

G
|
G

A
x
S

R

H

A

V

T

V

I

I

L
x
N

P

P

G

G

V

V

T

T

V

I

G

G

E

D

G

N

A

S

V

V

I

I

A

G

A
|
A

G

G

A

S

V

I

V

V

A

T

R

K

D

D

V

I

P

P

A

P

Y

N

D

V

V

V

V

A

G

A

G

G

V

V

P
|
P

A

C

K
x
R

K

V

I

I

G
x
R

E

E

R

I

N

N

-

D

R

R

D

D

I

K

R

H

Y

Y

L

Y

T

F

K

K

binding 4-nitrophenyl beta-D-galactopyranoside: S70 (≠ V62), Y82 (≠ V78), N84 (= N80), V90 (≠ D86), D92 (≠ R88), M126 (≠ A119)
binding coenzyme a: A104 (vs. gap), S110 (≠ Q102), T112 (≠ C104), W138 (= W131), G140 (= G133), S141 (≠ A134), N146 (≠ L139), A159 (= A152), P177 (= P170), R179 (≠ K172), R182 (≠ G175)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
33% identity, 77% coverage: 33:186/201 of query aligns to 40:194/201 of 1kruA

query
sites
1kruA

P

P

N

S

E

E

R

V

L

E

R

K

A

R

M

E

Y

S

L

L

R

I

R

K

V

E

M

M

K

F

V

A

A

T

V

V

G

G

Q

E

D

N

T

A

H
x
W

V

V

S

E

M

P

R

P

-

V

W

Y

F

F

V
x
S

T

Y

G

G

Y

-

P

-

V

-

R

-

A

S

N

N

V

I

T

H

I

I

G

G

N

R

N

N

C

F

V
x
Y

I

A

N
|
N

R

F

E

N

V

L

Y

T

L

I

D
x
V

G

D

R
x
D

V

Y

G

T

V

V

H

T

I

I

G

G

N

D

N

N

V

V

-
x
L

-

I

-

A

-

P

N

N

V

V

S

T

F

L

Q

S

T

V

C

T

I

G

L
x
H

S

P

L

V

H

H

D

E

H

L

N

R

S

K

P

-

D

N

F

G

I

E

A
x
M

V

Y

G

S

S

F

A

P

V

I

T

T

I

I

Q

G

D

N

H

N

V

V

W
|
W

I

I

G
|
G

A
x
S

R

H

A

V

T

V

I

I

L

N

P

P

G

G

V

V

T

T

V

I

G

G

E

D

G

N

A

S

V

V

I

I

A
x
G

A
|
A

G

G

A

S

V

I

V

V

A

T

R

K

D

D

V

I

P

P

A

P

Y

N

D

V

V

V

V

A

G

A

G

G

V

V

P
|
P

A

C

K

R

K

V

I

I

G
x
R

E

E

R

I

N

N

-

D

R

R

D

D

I

K

R

H

Y

Y

L

Y

T

F

K

K

binding coenzyme a: H114 (≠ L106), W138 (= W131), G140 (= G133), S141 (≠ A134), G158 (≠ A151), A159 (= A152), P177 (= P170), R182 (≠ G175)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: W62 (≠ H55), S70 (≠ V62), Y82 (≠ V78), Y82 (≠ V78), N84 (= N80), V90 (≠ D86), D92 (≠ R88), L102 (vs. gap), H114 (≠ L106), M126 (≠ A119)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
33% identity, 77% coverage: 33:186/201 of query aligns to 40:194/200 of 1krrA

query
sites
1krrA

P

P

N

S

E

E

R

V

L

E

R

K

A

R

M

E

Y

S

L

L

R

I

R

K

V

E

M

M

K

F

V

A

A

T

V

V

G

G

Q

E

D

N

T

A

H

W

V

V

S

E

M

P

R

P

-

V

W

Y

F

F

V

S

T

Y

G

G

Y

-

P

-

V

-

R

-

A

S

N

N

V

I

T

H

I

I

G

G

N

R

N

N

C

F

V

Y

I

A

N
|
N

R

F

E

N

V

L

Y

T

L

I

D

V

G

D

R

D

V

Y

G

T

V

V

H

T

I

I

G

G

N

D

N

N

V

V

-

L

-
x
I

-
x
A

-
x
P

N

N

V

V

S

T

F

L

Q

S

T

V

C
x
T

I

G

L
x
H

S

P

L

V

H

H

D

E

H

L

N

R

S

K

P

-

D

N

F

G

I

E

A

M

V

Y

G

S

S

F

A

P

V

I

T

T

I

I

Q

G

D

N

H

N

V

V

W

W

I

I

G
|
G

A
x
S

R

H

A

V

T

V

I

I

L
x
N

P

P

G

G

V

V

T

T

V

I

G

G

E

D

G

N

A

S

V

V

I

I

A
x
G

A
|
A

G

G

A

S

V

I

V

V

A

T

R

K

D

D

V

I

P

P

A

P

Y

N

D

V

V

V

V

A

G
x
A

G

G

V

V

P
|
P

A

C

K

R

K

V

I

I

G
x
R

E

E

R

I

N

N

-

D

R

R

D

D

I

K

R

H

Y

Y

L

Y

T

F

K

K

binding acetyl coenzyme *a: N84 (= N80), I103 (vs. gap), A104 (vs. gap), P105 (vs. gap), T112 (≠ C104), H114 (≠ L106), G140 (= G133), S141 (≠ A134), N146 (≠ L139), G158 (≠ A151), A159 (= A152), A174 (≠ G167), P177 (= P170), R182 (≠ G175)

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
35% identity, 53% coverage: 70:176/201 of query aligns to 74:186/186 of 4isxA

query
sites
4isxA

N

N

V

I

T

H

I

V

G

G

N

E

N

N

C

F

V

F

I

A

N
|
N

R

Y

E

D

V

C

Y

I

L

F

D

L

G

D

R

V

V

C

G

K

V

I

H

E

I

I

G

G

N

D

N

N

V

V

N

M

V

L

S
x
A

F

P

Q

N

T

V

C

Q

I

I

L

Y

S

T

L

A

H

Y

H

H

D

P

H

I

N

D

S

A

P

Q

-

L

-

R

-

N

D

S

F

G

I

I

A

E

V

Y

G

G

S

S

A

P

V

V

T

K

I

I

Q

G

D

D

H

N

V

V

W
|
W

I

I

G
|
G

A
x
G

R

G

A

V

T

I

I

I

L

T

P

P

G

G

V

I

T

T

V

I

G

G

E

D

G

N

A

V

V

V

I

I

A

G

A
|
A

G

G

A

S

V

V

A

T

R

K

D

D

V

I

P

P

A

P

Y

N

D

T

V

V

V

A

G
x
V

G

G

V

N

P

P

A

C

-

R

-

V

-

I

K
|
K

K

K

I

I

G

E

E

E

binding acetyl coenzyme *a: N84 (= N80), A104 (≠ S100), W138 (= W131), G140 (= G133), G141 (≠ A134), A159 (= A152), V174 (≠ G167), K182 (= K172)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
30% identity, 73% coverage: 26:172/201 of query aligns to 146:275/307 of A1ADJ6

query
sites
A1ADJ6

N

N

I

Y

V

V

A

R

R

I

F

H

P

K

N

N

E

S

R

K

L

I

R

K

A

G

-

D

M

I

Y

V

L

A

R

T

R

K

V

G

M

S

K

K

V

V

A

I

V

I

G

G

Q

R

D

R

T

T

H

T

V

I

S

G

M

-

R

-

W

-

F

-

V

-

T

A

G

G

Y

F

P

E

V

V

-

V

-

T

-

D

R

K

A

C

N

N

V

V

T

T

I

I

G

G

N

H

N

D

C

C

V

M

I

I

N

A

R

R

E

D

V

V

Y

I

L

L

D

R

G

A

R

S

V

D

G

G

V
x
H

H

P

I

I

G

-

N

-

V

-

N

-

V

-

S

-

F

-

Q

-

T

-

C

-

I

-

L

-

S

-

L

-

H

-

H

F

D

D

H

I

N

H

S

S

P

K

D

K

F

R

I

I

A

N

V

W

G

A

S

K

A

D

V

I

T

I

I

I

Q

S

D

S

H

Y

V

V

W
|
W

I

V

G

G

A

R

R

N

A

V

T

S

I

I

L

M

P

K

G

G

V

V

T

S

V

V

G

G

E

S

G

G

A

S

V

V

I

I

A

G

A

Y

G

G

A

S

V

I

V

V

A

T

R

K

D

D

V

V

P

P

A

S

Y

M

D

C

V

A

G

A

G

G

V

N

P

P

A

A

K

K

H210 (≠ V91) mutation to A: Loss of activity.
W234 (= W131) mutation to A: Loss of activity.

6u9cA The 2.2 a crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with acetyl coa
52% identity, 30% coverage: 122:182/201 of query aligns to 106:167/206 of 6u9cA

query
sites
6u9cA

S

S

A

G

V

D

T

T

I

I

Q

I

D

G

H

H

-

D

V

V

W
|
W

I

I

G

G

A

T

R

E

A

A

T

M

I

I

L

M

P

P

G

G

V

V

T

K

V

I

G

G

E

H

G

G

A

A

V

I

I

I

A
|
A

A

S

G

R

A

S

V

V

A

T

R

K

D

D

V

V

P

A

A

P

Y

Y

D

E

V

V

G

G

G

S

V

N

P

P

A

A

K

K

K

H

I
|
I

G

K

E

F

R

R

N

F

R

S

D

D

I

V

R

E

binding acetyl coenzyme *a: W116 (= W131), A136 (= A151), I159 (= I174)

6pubA Crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with crystal violet
52% identity, 30% coverage: 122:182/201 of query aligns to 109:170/210 of 6pubA

query
sites
6pubA

S

S

A

G

V

D

T

T

I

I

Q

I

D

G

H

H

-

D

V

V

W

W

I

I

G

G

A

T

R

E

A

A

T

M

I

I

L

M

P

P

G

G

V

V

T

K

V

I

G

G

E

H

G

G

A

A

V

I

I

I

A

A

A

S

G

R

A

S

V

V

A

T

R

K

D

D

V

V

P

A

A

P

Y

Y

D

E

V

V

G

G

G

S

V

N

P

P

A

A

K

K

K

H

I

I

G

K

E

F

R

R

N

F

R

S

D

D

I

V

R

E

Sites not aligning to the query:

binding crystal violet: 4, 7, 9, 10, 11, 36, 37

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
57% identity, 27% coverage: 121:174/201 of query aligns to 130:183/188 of 3igjC

query
sites
3igjC

G

G

S

K

A

P

V

V

T

K

I

I

Q

G

D

N

H

N

V

V

W

W

I

V

G
|
G

A

G

R

G

A

A

T

I

I

I

L
x
N

P

P

G

G

V

V

T

S

V

I

G

G

E

D

G

N

A

A

V

V

I

I

A
|
A

A
x
S

G

G

A

A

V

V

A
x
T

R

K

D

D

V

V

P

P

A

N

Y

N

D

V

V

V

G

G

V
x
N

P

P

A

A

K

K

K

V

I

I

binding acetyl coenzyme *a: G142 (= G133), N148 (≠ L139), A160 (= A151), S161 (≠ A152), T166 (≠ A157), N178 (≠ V169)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84, 106, 112, 114, 116

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
46% identity, 31% coverage: 118:180/201 of query aligns to 107:169/211 of 4hurA

query
sites
4hurA

I

L

A

P

V

L

G
x
K

S

G

A

D

V

I

T

E

I

I

Q

G

D

N

H

D

V

V

W
|
W

I
|
I

G
|
G

A
x
R

R

D

A

V

T

T

I

I

L
x
M

P

P

G

G

V

V

T

K

V

I

G

G

E

D

G

G

A

A

V

I

I

I

A
|
A

G

E

A

A

V

V

A
x
T

R

K

D

N

V

V

P

A

A

P

Y

Y

D

S

V

I

V

V

G
|
G

G

G

V

N

P
|
P

A

L

K

K

K

F

I
|
I

G
x
R

E

K

R

R

N

F

R

S

D

D

binding acetyl coenzyme *a: K110 (≠ G121), W120 (= W131), I121 (= I132), G122 (= G133), R123 (≠ A134), M128 (≠ L139), A140 (= A151), A141 (= A152), T146 (≠ A157), G156 (= G167), P159 (= P170), I163 (= I174), R164 (≠ G175)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51, 67, 68, 69, 79, 80

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
46% identity, 31% coverage: 118:180/201 of query aligns to 107:169/212 of 4husA

query
sites
4husA

I

L

A

P

V

L

G

K

S

G

A

D

V

I

T

E

I

I

Q

G

D

N

H

D

V

V

W

W

I

I

G

G

A

R

R

D

A

V

T

T

I

I

L

M

P

P

G

G

V

V

T

K

V

I

G

G

E

D

G

G

A

A

V

I

I

I

A

A

G

E

A

A

V

V

A

T

R

K

D

N

V

V

P

A

A

P

Y

Y

D

S

V

I

V

V

G

G

V

N

P

P

A

L

K

K

K

F

I

I

G

R

E

K

R

R

N

F

R

S

D

D

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
46% identity, 31% coverage: 118:180/201 of query aligns to 107:169/207 of 6x3cA

query
sites
6x3cA

I

L

A

P

V

L

G

K

S

G

A

D

V

I

T

E

I

I

Q

G

D

N

H

D

V

V

W
|
W

I

I

G
|
G

A
x
R

R

D

A

V

T

T

I

I

L
x
M

P

P

G

G

V

V

T

K

V

I

G

G

E

D

G

G

A

A

V

I

I

I

A
|
A

G

E

A

A

V

V

A

T

R

K

D

N

V

V

P

A

A

P

Y

Y

D

S

V

I

V

V

G

G

V

N

P

P

A

L

K

K

K

F

I

I

G

R

E

K

R

R

N

F

R

S

D

D

binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: W120 (= W131), G122 (= G133), R123 (≠ A134), M128 (≠ L139), A140 (= A151), A141 (= A152)

Sites not aligning to the query:

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
46% identity, 31% coverage: 118:180/201 of query aligns to 107:169/206 of 6x3jA

query
sites
6x3jA

I
x
L

A

P

V

L

G

K

S

G

A

D

V

I

T

E

I

I

Q

G

D

N

H

D

V

V

W
|
W

I

I

G
|
G

A

R

R

D

A

V

T

T

I

I

L

M

P

P

G

G

V

V

T

K

V

I

G

G

E

D

G

G

A

A

V

I

I

I

A

A

G

E

A

A

V

V

A

T

R

K

D

N

V

V

P

A

A

P

Y

Y

D

S

V

I

V

V

G

G

V

N

P

P

A

L

K

K

K

F

I

I

G

R

E

K

R

R

N

F

R

S

D

D

Sites not aligning to the query:

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
46% identity, 31% coverage: 118:180/201 of query aligns to 107:169/203 of 6x3cE

query
sites
6x3cE

I

L

A

P

V

L

G

K

S

G

A

D

V

I

T

E

I

I

Q

G

D

N

H

D

V

V

W
|
W

I

I

G
|
G

A

R

R

D

A

V

T

T

I

I

L

M

P

P

G

G

V

V

T

K

V

I

G

G

E

D

G

G

A

A

V

I

I

I

A
|
A

G

E

A

A

V

V

A

T

R

K

D

N

V

V

P

A

A

P

Y

Y

D

S

V

I

V

V

G

G

V
x
N

P
|
P

A

L

K

K

K

F

I

I

G

R

E

K

R

R

N

F

R

S

D

D

binding magnesium ion: N158 (≠ V169), P159 (= P170)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: W120 (= W131), G122 (= G133), A140 (= A151), A141 (= A152)

Sites not aligning to the query:

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
37% identity, 57% coverage: 61:174/201 of query aligns to 93:219/259 of 3fscA

query
sites
3fscA

F

F

V

Q

T

T

G

G

Y

H

P

K

V

V

-

T

-

I

R

R

A

E

N

N

V

T

T

K

I

I

G

G

N

N

C

V

V
x
K

I

I

-
x
G

-
x
T

-

L

-

S

-

D

-

I

N

Q

R

H

E

H

V

V

Y

Y

L

I

D

G

G

N

R

Y

V

V

G

N

V

I

H
|
H

I

-

G

-

N
x
S

N

N

V

V

N

F

V

V

S

G

F

E

Q

K

T

S

C

I

I

I

-

K

-

D

-

F

-

V

-

W

-

L

-
x
F

-

P

-

H

L

V

S

V

L

L

H

T

H

N

D

D

H

P

N

T

S

P

P

P

D

S

F

N

I

E

A

L

V

L

G

G

S

-

A

-

V

V

T

T

I

I

Q

E

D

L

H

F

V

A

W

V

I

I

G
x
A

A
|
A

R

R

A

S

T

V

I

V

L

L

P

P

G

G

V

I

T

H

V

I

G

N

E

E

G

D

A

A

V

L

I

V

A
x
G

A
|
A

G

G

A

A

V

V

A

T

R

K

D

D

V

V

P

P

A

K

Y

E

D

T

V

V

G
x
V

G

G

V

N

P

P

A

A

K

R

K

E

I

I

binding coenzyme a: F150 (vs. gap), A178 (≠ G133), A179 (= A134), G196 (≠ A151), A197 (= A152), V212 (≠ G167)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: K112 (≠ V78), G114 (vs. gap), T115 (vs. gap), H132 (= H92), S133 (≠ N95)

Sites not aligning to the query:

3fs8A Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d- glucose n-acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with acetyl-coa (see paper)
37% identity, 57% coverage: 61:174/201 of query aligns to 93:219/259 of 3fs8A

query
sites
3fs8A

F

F

V

Q

T

T

G

G

Y

H

P

K

V

V

-

T

-

I

R
|
R

A

E

N

N

V

T

T

K

I

I

G

G

N

N

C

V

V

K

I

I

-

G

-

T

-

L

-

S

-

D

-

I

N

Q

R

H

E

H

V

V

Y

Y

L

I

D

G

G

N

R

Y

V

V

G

N

V

I

H

H

I

-

G

-

N

S

N

N

V

V

N

F

V

V

S

G

F

E

Q

K

T

S

C

I

I

I

-

K

-

D

-

F

-

V

-

W

-

L

-
x
F

-
x
P

-

H

L

V

S

V

L

L

H

T

H

N

D

D

H

P

N

T

S

P

P

P

D

S

F

N

I

E

A

L

V

L

G

G

S

-

A

-

V

V

T

T

I

I

Q

E

D

L

H

F

V

A

W

V

I

I

G
x
A

A
|
A

R

R

A

S

T

V

I

V

L

L

P

P

G

G

V

I

T

H

V

I

G

N

E

E

G

D

A

A

V

L

I

V

A
x
G

A
|
A

G

G

A

A

V

V

A

T

R

K

D

D

V

V

P

P

A

K

Y

E

D

T

V

V

G
x
V

G

G

V

N

P

P

A

A

K

R

K

E

I

I

binding acetyl coenzyme *a: F150 (vs. gap), P151 (vs. gap), A178 (≠ G133), A179 (= A134), G196 (≠ A151), A197 (= A152), V212 (≠ G167)
binding thymine: R102 (= R68)

Sites not aligning to the query:

2xatA Complex of the hexapeptide xenobiotic acetyltransferase with chloramphenicol and desulfo-coenzyme a (see paper)
31% identity, 64% coverage: 70:197/201 of query aligns to 54:183/208 of 2xatA

query
sites
2xatA

N

K

V

L

T

V

I

I

G

G

N

S

N

F

C

C

V

S

I

I

N

G

R

-

E

-

V

-

Y

-

L

-

D

S

G

G

R

A

V

A

G

F

V

I

H

M

I

A

G

G

N

N

N

Q

V

G

N

H

V

R

S

A

F

E

Q

W

T

A

C

S

I

T

L

F

S

P

L

F

H

H

H

F

D

M

H

H

N

E

S

E

P

P

D

A

F

F

I

A

A

G

V

A

-

V

-

N

-

G

-

Y

-

Q

-

P

G

A

S

G

A

D

V

T

T

L

I

I

Q

G

D

H

H

E

V

V

W

W

I

I

G
|
G

A
x
T

R

E

A

A

T

M

I

F

L

M

P

P

G

G

V

V

T

R

V

V

G

G

E

H

G

G

A

A

V

I

I

I

A
x
G

A
x
S

G

R

A

A

V

L

V

V

A

T

R

G

D

D

V

V

P

E

A

P

Y

Y

D

A

V

I

V

V

G
|
G

V

N

P

P

A

A

K

R

K

T

I
|
I

G

R

E

K

R

R

-

F

-

S

N

D

R

G

D

D

I

I

R

Q

Y

N

L

L

T

L

K

E

F

M

S

A

P

-

Y

W

F

W

D

D

S

W

D

P

I

L

F

A

D

D

binding desulfo-coenzyme a: G118 (= G133), T119 (≠ A134), G136 (≠ A151), S137 (≠ A152), G152 (= G167), G153 (= G168), I159 (= I174)

Sites not aligning to the query:

binding chloramphenicol: 6, 7, 8, 28

3fsbA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-quinovose (see paper)
37% identity, 57% coverage: 61:174/201 of query aligns to 93:219/260 of 3fsbA

query
sites
3fsbA

F

F

V

Q

T

T

G

G

Y

H

P

K

V

V

-

T

-

I

R

R

A

E

N

N

V

T

T

K

I

I

G

G

N

N

C

V

V

K

I

I

-
x
G

-

T

-

L

-

S

-

D

-

I

N

Q

R

H

E

H

V

V

Y

Y

L

I

D

G

G

N

R

Y

V

V

G

N

V

I

H
|
H

I

-

G

-

N

S

N

N

V

V

N

F

V

V

S

G

F

E

Q

K

T

S

C

I

I

I

-

K

-

D

-

F

-

V

-

W

-

L

-
x
F

-

P

-

H

L

V

S

V

L

L

H

T

H

N

D

D

H

P

N

T

S

P

P

P

D

S

F

N

I

E

A

L

V

L

G

G

S

-

A

-

V

V

T

T

I

I

Q

E

D

L

H

F

V

A

W

V

I

I

G
x
A

A
|
A

R

R

A

S

T

V

I

V

L

L

P

P

G

G

V

I

T

H

V

I

G

N

E

E

G

D

A

A

V

L

I

V

A
x
G

A
|
A

G

G

A

A

V

V

A

T

R

K

D

D

V

V

P

P

A

K

Y

E

D

T

V

V

G
x
V

G

G

V

N

P

P

A

A

K

R

K

E

I

I

binding coenzyme a: F150 (vs. gap), A178 (≠ G133), A179 (= A134), G196 (≠ A151), A197 (= A152), V212 (≠ G167)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: G114 (vs. gap), H132 (= H92), F150 (vs. gap)

Sites not aligning to the query:

binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 241, 243

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
40% identity, 31% coverage: 116:177/201 of query aligns to 106:167/204 of 1mrlA

query
sites
1mrlA

D

D

F

Q

I
x
L

A

P

V

I

G

K

S

G

A

D

V

T

T

I

I

I

Q

G

D

N

H

D

V

V

W

W

I

I

G

G

A

K

R

D

A

V

T

V

I

I

L

M

P

P

G

G

V

V

T

K

V

I

G

G

E

D

G

G

A

A

V

I

I

V

A

A

G

N

A

S

V

V

A

V

R

K

D

D

V

I

P

A

A

P

Y

Y

D

M

V

L

V

A

G

G

V

N

P

P

A

A

K

N

K

E

I

I

G

K

E

Q

R

R

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: L108 (≠ I118)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56, 81, 82, 93, 102

Query Sequence

>WP_057508416.1 NCBI__GCF_001431535.1:WP_057508416.1
MKKLKVWLLHQRFFYVLMGLKYYVGNNIVARFPNERLRAMYLRRVMKVAVGQDTHVSMRW
FVTGYPVRANVTIGNNCVINREVYLDGRVGVHIGNNVNVSFQTCILSLHHDHNSPDFIAV
GSAVTIQDHVWIGARATILPGVTVGEGAVIAAGAVVARDVPAYDVVGGVPAKKIGERNRD
IRYLTKFSPYFDSDIFDETAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory