SitesBLAST
Comparing WP_057508536.1 NCBI__GCF_001431535.1:WP_057508536.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
45% identity, 97% coverage: 10:319/320 of query aligns to 22:335/339 of Q7XSN8
- E219 (= E207) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (= D213) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
5cvcA Structure of maize serine racemase (see paper)
44% identity, 97% coverage: 10:319/320 of query aligns to 6:319/329 of 5cvcA
- active site: K52 (= K56), S77 (= S81), E203 (= E207), A207 (= A211), D209 (= D213), G231 (= G235), V306 (≠ L305), S307 (= S306)
- binding magnesium ion: E203 (= E207), A207 (= A211), D209 (= D213)
- binding pyridoxal-5'-phosphate: F51 (= F55), K52 (= K56), N79 (= N83), S178 (≠ G182), G179 (= G183), G180 (= G184), G181 (= G185), L232 (= L236), E275 (= E279), S307 (= S306), G308 (= G307)
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
39% identity, 96% coverage: 12:317/320 of query aligns to 9:322/322 of 7nbgAAA
- active site: K53 (= K56), S81 (= S81), E207 (= E207), A211 (= A211), D213 (= D213), G236 (= G235), L309 (= L305), S310 (= S306)
- binding calcium ion: E207 (= E207), A211 (= A211), D213 (= D213)
- binding pyridoxal-5'-phosphate: F52 (= F55), K53 (= K56), N83 (= N83), G182 (= G182), G183 (= G183), G184 (= G184), G185 (= G185), M186 (≠ L186), G236 (= G235), V237 (≠ L236), T282 (≠ S281), S310 (= S306), G311 (= G307)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (= S81), G85 (= G85), Q86 (≠ A86), I101 (≠ V101), K111 (= K111), I115 (= I115), Y118 (≠ H118)
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
39% identity, 96% coverage: 12:317/320 of query aligns to 9:322/323 of 7nbfAAA
- active site: K53 (= K56), S81 (= S81), E207 (= E207), A211 (= A211), D213 (= D213), G236 (= G235), L309 (= L305), S310 (= S306)
- binding calcium ion: E207 (= E207), A211 (= A211), D213 (= D213)
- binding magnesium ion: N244 (≠ P243)
- binding pyridoxal-5'-phosphate: F52 (= F55), K53 (= K56), N83 (= N83), G182 (= G182), G183 (= G183), G184 (= G184), G185 (= G185), M186 (≠ L186), G236 (= G235), V237 (≠ L236), T282 (≠ S281), S310 (= S306), G311 (= G307)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (≠ A24), L22 (≠ V25), T23 (= T26), P24 (= P27), L26 (= L29), T27 (≠ R30), F46 (≠ L49)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
39% identity, 96% coverage: 12:317/320 of query aligns to 9:322/323 of 7nbdAAA
- active site: K53 (= K56), S81 (= S81), E207 (= E207), A211 (= A211), D213 (= D213), G236 (= G235), L309 (= L305), S310 (= S306)
- binding calcium ion: E207 (= E207), A211 (= A211), D213 (= D213)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (= W271), L278 (≠ T277), V314 (= V310), L316 (≠ I312)
- binding magnesium ion: N244 (≠ P243)
- binding pyridoxal-5'-phosphate: F52 (= F55), K53 (= K56), N83 (= N83), G182 (= G182), G183 (= G183), G184 (= G184), G185 (= G185), M186 (≠ L186), G236 (= G235), V237 (≠ L236), E280 (= E279), T282 (≠ S281), S310 (= S306), G311 (= G307)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
39% identity, 96% coverage: 12:317/320 of query aligns to 9:322/323 of 7nbcCCC
- active site: K53 (= K56), S81 (= S81), E207 (= E207), A211 (= A211), D213 (= D213), G236 (= G235), L309 (= L305), S310 (= S306)
- binding biphenyl-4-ylacetic acid: T78 (= T78), H79 (= H79), H84 (= H84), V148 (= V148), H149 (= H149), P150 (= P150)
- binding calcium ion: E207 (= E207), A211 (= A211), D213 (= D213)
- binding pyridoxal-5'-phosphate: F52 (= F55), K53 (= K56), N83 (= N83), G182 (= G182), G183 (= G183), G184 (= G184), G185 (= G185), M186 (≠ L186), G236 (= G235), V237 (≠ L236), T282 (≠ S281), S310 (= S306), G311 (= G307)
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
39% identity, 96% coverage: 12:317/320 of query aligns to 9:322/323 of 7nbcAAA
- active site: K53 (= K56), S81 (= S81), E207 (= E207), A211 (= A211), D213 (= D213), G236 (= G235), L309 (= L305), S310 (= S306)
- binding calcium ion: E207 (= E207), A211 (= A211), D213 (= D213)
- binding magnesium ion: N244 (≠ P243)
- binding pyridoxal-5'-phosphate: F52 (= F55), K53 (= K56), N83 (= N83), G182 (= G182), G183 (= G183), G184 (= G184), G185 (= G185), M186 (≠ L186), G236 (= G235), V237 (≠ L236), T282 (≠ S281), S310 (= S306), G311 (= G307)
Sites not aligning to the query:
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
39% identity, 94% coverage: 12:312/320 of query aligns to 9:316/320 of 7nbhAAA
- active site: K53 (= K56), S81 (= S81), E207 (= E207), A211 (= A211), D213 (= D213), G236 (= G235), L309 (= L305), S310 (= S306)
- binding calcium ion: E207 (= E207), A211 (= A211), D213 (= D213)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (= S81), G85 (= G85), Q86 (≠ A86), K111 (= K111), I115 (= I115), Y118 (≠ H118), D235 (= D234), P281 (= P280), N313 (= N309), V314 (= V310), D315 (= D311)
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
39% identity, 96% coverage: 12:317/320 of query aligns to 10:318/322 of 3l6bA
- active site: K54 (= K56), S77 (= S81), E203 (= E207), A207 (= A211), D209 (= D213), G232 (= G235), T278 (≠ S281), L305 (= L305), S306 (= S306)
- binding malonate ion: K54 (= K56), S76 (= S80), S77 (= S81), N79 (= N83), H80 (= H84), R128 (= R132), G232 (= G235)
- binding manganese (ii) ion: E203 (= E207), A207 (= A211), D209 (= D213)
- binding pyridoxal-5'-phosphate: F53 (= F55), K54 (= K56), N79 (= N83), G178 (= G182), G179 (= G183), G180 (= G184), G181 (= G185), M182 (≠ L186), V233 (≠ L236), E276 (= E279), T278 (≠ S281), S306 (= S306), G307 (= G307)
7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
39% identity, 94% coverage: 12:311/320 of query aligns to 9:310/310 of 7nbgDDD
- active site: K53 (= K56), S76 (= S81), E202 (= E207), A206 (= A211), D208 (= D213), G231 (= G235), L304 (= L305), S305 (= S306)
- binding calcium ion: E202 (= E207), A206 (= A211), D208 (= D213)
- binding magnesium ion: N239 (≠ P243)
- binding ortho-xylene: S76 (= S81), Q81 (≠ A86), I96 (≠ V101), Y113 (≠ H118)
- binding pyridoxal-5'-phosphate: F52 (= F55), K53 (= K56), N78 (= N83), G177 (= G182), G178 (= G183), G179 (= G184), G180 (= G185), M181 (≠ L186), G231 (= G235), V232 (≠ L236), E275 (= E279), T277 (≠ S281), S305 (= S306), G306 (= G307)
Sites not aligning to the query:
6zspAAA serine racemase bound to atp and malonate. (see paper)
39% identity, 96% coverage: 12:317/320 of query aligns to 9:315/320 of 6zspAAA
- active site: K53 (= K56), S74 (= S81), E200 (= E207), A204 (= A211), D206 (= D213), G229 (= G235), L302 (= L305), S303 (= S306)
- binding adenosine-5'-triphosphate: S28 (= S31), S29 (≠ Q32), I30 (≠ T33), K48 (≠ R51), T49 (≠ S52), Q79 (≠ A86), Y111 (≠ H118), E266 (≠ Q272), R267 (≠ V273), K269 (= K275), N306 (= N309)
- binding magnesium ion: E200 (= E207), A204 (= A211), D206 (= D213)
- binding malonate ion: K53 (= K56), S73 (= S80), S74 (= S81), N76 (= N83), H77 (= H84), R125 (= R132), G229 (= G235), S232 (≠ G238)
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
42% identity, 94% coverage: 12:313/320 of query aligns to 9:311/319 of A4F2N8
- K53 (= K56) mutation to A: Loss of enzymatic activity.
Q9QZX7 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Mus musculus (Mouse) (see paper)
40% identity, 96% coverage: 12:317/320 of query aligns to 12:324/339 of Q9QZX7
- C113 (≠ A110) modified: S-nitrosocysteine; mutation to S: Abolishes S-nitrosylation.
3hmkA Crystal structure of serine racemase (see paper)
39% identity, 95% coverage: 12:315/320 of query aligns to 10:320/321 of 3hmkA
- active site: K54 (= K56), S82 (= S81), E208 (= E207), A212 (= A211), D214 (= D213), G237 (= G235), T283 (≠ S281), L310 (= L305), S311 (= S306)
- binding manganese (ii) ion: E208 (= E207), A212 (= A211), D214 (= D213)
- binding pyridoxal-5'-phosphate: F53 (= F55), K54 (= K56), N84 (= N83), G183 (= G182), G184 (= G183), G185 (= G184), G186 (= G185), M187 (≠ L186), G237 (= G235), V238 (≠ L236), T283 (≠ S281), S311 (= S306), G312 (= G307)
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 96% coverage: 7:313/320 of query aligns to 8:315/323 of O59791
- S82 (= S81) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
36% identity, 96% coverage: 7:313/320 of query aligns to 3:310/318 of 1wtcA
- active site: K52 (= K56), S77 (= S81), E203 (= E207), G207 (≠ A211), D209 (= D213), G231 (= G235), I302 (≠ L305), S303 (= S306)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ A24), K47 (≠ R51), M48 (≠ S52), A109 (= A113), A110 (≠ N114), Y114 (≠ H118)
- binding magnesium ion: E203 (= E207), G207 (≠ A211), D209 (= D213)
- binding pyridoxal-5'-phosphate: F51 (= F55), K52 (= K56), N79 (= N83), G178 (= G182), G179 (= G183), G180 (= G184), G181 (= G185), G231 (= G235), E276 (= E279), T278 (≠ S281), S303 (= S306)
1v71A Crystal structure of s.Pombe serine racemase
36% identity, 96% coverage: 7:313/320 of query aligns to 3:310/318 of 1v71A
- active site: K52 (= K56), S77 (= S81), E203 (= E207), G207 (≠ A211), D209 (= D213), G231 (= G235), I302 (≠ L305), S303 (= S306)
- binding magnesium ion: E203 (= E207), G207 (≠ A211), D209 (= D213)
- binding pyridoxal-5'-phosphate: F51 (= F55), K52 (= K56), N79 (= N83), G178 (= G182), G179 (= G183), G180 (= G184), G181 (= G185), G231 (= G235), E276 (= E279), T278 (≠ S281), S303 (= S306), G304 (= G307)
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
36% identity, 96% coverage: 7:313/320 of query aligns to 4:311/319 of 2zr8A
- active site: K53 (= K56), S78 (= S81), E204 (= E207), G208 (≠ A211), D210 (= D213), G232 (= G235), I303 (≠ L305), S304 (= S306)
- binding magnesium ion: E204 (= E207), G208 (≠ A211), D210 (= D213)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F55), K53 (= K56), S77 (= S80), S78 (= S81), N80 (= N83), H81 (= H84), P147 (= P150), G179 (= G182), G180 (= G183), G181 (= G184), G182 (= G185), G232 (= G235), E277 (= E279), T279 (≠ S281), S304 (= S306)
- binding serine: S78 (= S81), R129 (= R132), D231 (= D234), G232 (= G235), A233 (≠ L236), Q234 (≠ R237), T235 (≠ G238)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
36% identity, 96% coverage: 7:313/320 of query aligns to 4:311/319 of 2zpuA
- active site: K53 (= K56), S78 (= S81), E204 (= E207), G208 (≠ A211), D210 (= D213), G232 (= G235), I303 (≠ L305), S304 (= S306)
- binding magnesium ion: E204 (= E207), G208 (≠ A211), D210 (= D213)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F55), K53 (= K56), S77 (= S80), S78 (= S81), N80 (= N83), H81 (= H84), P147 (= P150), G179 (= G182), G180 (= G183), G181 (= G184), G182 (= G185), G232 (= G235), E277 (= E279), T279 (≠ S281), S304 (= S306)
1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
42% identity, 96% coverage: 7:312/320 of query aligns to 1:306/308 of 1ve5A
- active site: K50 (= K56), S56 (≠ N62), S72 (= S81), E200 (= E207), A204 (= A211), D206 (= D213), G229 (= G235), L299 (= L305), S300 (= S306)
- binding calcium ion: E200 (= E207), A204 (= A211), D206 (= D213)
- binding pyridoxal-5'-phosphate: F49 (= F55), K50 (= K56), N74 (= N83), G175 (= G182), G176 (= G183), G177 (= G184), G178 (= G185), E274 (= E279), T276 (≠ S281), S300 (= S306), G301 (= G307)
Query Sequence
>WP_057508536.1 NCBI__GCF_001431535.1:WP_057508536.1
MNEALPPPAQAVLEAAARIAPHAAVTPVLRSQTLDALAGAQLAFKAEHLQRSGAFKFRGA
CNAVWSLSADRAPAGVVTHSSGNHGAALALAARTRGIACHVVVPDGAVAAKLANIARHGA
TLWRCAPTIAAREAVCAQVQADTGATLVHPYADAAVIAGQGTAVLELLQAEGPFDVVVVP
VGGGGLAAGTALALQQVSPHTRLVLAEPAGAADTARSLQAGERRIDFVPDTVCDGLRGTL
GAPNFQLLQAAGAEVIVVDDAATVAAMRLLWQVLKQTVEPSSAIALAAVLAQPQRFAGLR
VGLVLSGGNVDIDALPWSAA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory