SitesBLAST
Comparing WP_057509011.1 NCBI__GCF_001431535.1:WP_057509011.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3fslA Crystal structure of tyrosine aminotransferase tripple mutant (p181q, r183g,a321k) from escherichia coli at 2.35 a resolution
50% identity, 99% coverage: 3:397/400 of query aligns to 1:396/397 of 3fslA
- active site: W131 (= W132), D212 (= D213), A214 (= A215), K247 (= K249)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G103 (= G104), G104 (= G105), S105 (= S106), W131 (= W132), N184 (= N185), D212 (= D213), A214 (= A215), Y215 (= Y216), S244 (= S246), S246 (= S248), K247 (= K249), R255 (= R257)
3tatA Tyrosine aminotransferase from e. Coli (see paper)
50% identity, 99% coverage: 3:397/400 of query aligns to 1:396/397 of 3tatA
- active site: W131 (= W132), D212 (= D213), A214 (= A215), K247 (= K249)
- binding pyridoxal-5'-phosphate: G103 (= G104), G104 (= G105), S105 (= S106), H179 (= H180), N184 (= N185), A214 (= A215), S246 (= S248), K247 (= K249), R255 (= R257)
6zvgA Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 cocrystalized with substrate analog - l-indole-3-lactic acid (see paper)
46% identity, 99% coverage: 3:399/400 of query aligns to 1:397/398 of 6zvgA
- binding 3-(indol-3-yl) lactate: D11 (= D13), I13 (= I15), L14 (= L16), I33 (≠ V35), G34 (= G36), W130 (= W132), K246 (= K249), R374 (= R376)
- binding pyridoxal-5'-phosphate: G102 (= G104), G103 (= G105), S104 (= S106), W130 (= W132), N183 (= N185), D211 (= D213), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
6zurA Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 cocrystalized with substrate analog - l-p-hydroxyphenyllactic acid (see paper)
46% identity, 99% coverage: 3:399/400 of query aligns to 1:397/398 of 6zurA
- binding pyridoxal-5'-phosphate: G102 (= G104), G103 (= G105), S104 (= S106), W130 (= W132), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
- binding (2S)-2-hydroxy-3-(4-hydroxyphenyl)propanoic acid: G34 (= G36), W130 (= W132), R374 (= R376)
6zupA Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 cocrystalized with substrate analog - l-(-)-3-phenyllactic acid (see paper)
46% identity, 99% coverage: 3:399/400 of query aligns to 1:397/398 of 6zupA
- binding alpha-hydroxy-beta-phenyl-propionic acid: I13 (= I15), I33 (≠ V35), G34 (= G36), W130 (= W132), K246 (= K249), R374 (= R376)
- binding pyridoxal-5'-phosphate: G102 (= G104), G103 (= G105), S104 (= S106), W130 (= W132), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
4rkdC Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 cocrystalized with aspartic acid (see paper)
46% identity, 99% coverage: 3:399/400 of query aligns to 1:397/398 of 4rkdC
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding oxaloacetate ion: Y65 (= Y67), R280 (= R283)
- binding pyridoxal-5'-phosphate: G102 (= G104), G103 (= G105), S104 (= S106), W130 (= W132), N183 (= N185), D211 (= D213), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
4rkdA Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 cocrystalized with aspartic acid (see paper)
46% identity, 99% coverage: 3:399/400 of query aligns to 1:397/398 of 4rkdA
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethylene)-amino]-succinic acid: G34 (= G36), G102 (= G104), G103 (= G105), S104 (= S106), W130 (= W132), D211 (= D213), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
- binding pyridoxal-5'-phosphate: G102 (= G104), G103 (= G105), S104 (= S106), W130 (= W132), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
4rkcA Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 (see paper)
46% identity, 99% coverage: 3:399/400 of query aligns to 1:397/398 of 4rkcA
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G102 (= G104), G103 (= G105), S104 (= S106), W130 (= W132), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
1ahxA Aspartate aminotransferase hexamutant (see paper)
47% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 1ahxA
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding hydrocinnamic acid: I13 (= I15), L14 (= L16), G34 (= G36), W130 (= W132), K246 (= K249), R374 (= R376)
- binding pyridoxal-5'-phosphate: G102 (= G104), G103 (= G105), S104 (= S106), W130 (= W132), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
1ahgA Aspartate aminotransferase hexamutant (see paper)
47% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 1ahgA
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding pyridoxal-5'-phosphate: G103 (= G105), S104 (= S106), W130 (= W132), N183 (= N185), D211 (= D213), S243 (= S246), S245 (= S248), R254 (= R257)
- binding tyrosine: D11 (= D13), I13 (= I15), L14 (= L16), W130 (= W132), R374 (= R376)
1ahfA Aspartate aminotransferase hexamutant (see paper)
47% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 1ahfA
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding indolylpropionic acid: I13 (= I15), L14 (= L16), I33 (≠ V35), G34 (= G36), W130 (= W132), R374 (= R376)
- binding pyridoxal-5'-phosphate: G102 (= G104), G103 (= G105), S104 (= S106), W130 (= W132), N183 (= N185), D211 (= D213), A213 (= A215), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
5vwrA E.Coli aspartate aminotransferase-(1r,3s,4s)-3-amino-4- fluorocyclopentane-1-carboxylic acid (fcp)-alpha-ketoglutarate (see paper)
46% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 5vwrA
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-glutamic acid: I13 (= I15), G34 (= G36), G102 (= G104), G103 (= G105), T104 (≠ S106), W130 (= W132), N183 (= N185), D211 (= D213), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257), R374 (= R376)
5t4lA Plp and gaba trigger gabr-mediated transcription regulation in bacillus subsidies via external aldimine formation (see paper)
46% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 5t4lA
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding (4R)-4-amino-6-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}hexanoic acid: G102 (= G104), G103 (= G105), T104 (≠ S106), W130 (= W132), D211 (= D213), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
3qpgA Crystal structures of escherichia coli aspartate aminotransferase reconstituted with 1-deaza-pyridoxal 5'-phosphate: internal aldimine and stable l-aspartate external aldimine (see paper)
46% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 3qpgA
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding (E)-N-{2-hydroxy-3-methyl-6-[(phosphonooxy)methyl]benzylidene}-L-aspartic acid: I13 (= I15), G34 (= G36), G102 (= G104), G103 (= G105), T104 (≠ S106), W130 (= W132), N183 (= N185), D211 (= D213), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257), R374 (= R376)
3pa9A Mechanism of inactivation of e. Coli aspartate aminotransferase by (s)-4-amino-4,5-dihydro-2-furancarboxylic acid (s-adfa) ph 7.5 (see paper)
46% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 3pa9A
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding 4-aminofuran-2-carboxylic acid: G34 (= G36), W130 (= W132), K246 (= K249), F348 (= F350), R374 (= R376)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G103 (= G105), T104 (≠ S106), W130 (= W132), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257)
1x2aA Crystal structure of e.Coli aspat complexed with n-phosphopyridoxyl-d- glutamic acid (see paper)
46% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 1x2aA
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-d-glutamic acid: I33 (≠ V35), G34 (= G36), Y65 (= Y67), G102 (= G104), G103 (= G105), T104 (≠ S106), W130 (= W132), N183 (= N185), D211 (= D213), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257), R280 (= R283), F348 (= F350), R374 (= R376)
1x29A Crystal structure of e.Coli aspat complexed with n-phosphopyridoxyl-2- methyl-l-glutamic acid (see paper)
46% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 1x29A
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-2-methyl-l-glutamic acid: I13 (= I15), G34 (= G36), Y65 (= Y67), G103 (= G105), T104 (≠ S106), W130 (= W132), N183 (= N185), D211 (= D213), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257), R280 (= R283), F348 (= F350), R374 (= R376)
1x28A Crystal structure of e.Coli aspat complexed with n-phosphopyridoxyl-l- glutamic acid (see paper)
46% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 1x28A
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I13 (= I15), Y65 (= Y67), G103 (= G105), T104 (≠ S106), W130 (= W132), N183 (= N185), D211 (= D213), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257), R280 (= R283), F348 (= F350), R374 (= R376)
1cq8A Aspartate aminotransferase (E.C. 2.6.1.1) complexed with c6-pyridoxal- 5p-phosphate (see paper)
46% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 1cq8A
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: I33 (≠ V35), G34 (= G36), G103 (= G105), T104 (≠ S106), W130 (= W132), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257), R374 (= R376)
1cq7A Aspartate aminotransferase (E.C. 2.6.1.1) complexed with c5-pyridoxal- 5p-phosphate (see paper)
46% identity, 99% coverage: 3:397/400 of query aligns to 1:395/396 of 1cq7A
- active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K249)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: I13 (= I15), I33 (≠ V35), G34 (= G36), G103 (= G105), T104 (≠ S106), W130 (= W132), N183 (= N185), D211 (= D213), Y214 (= Y216), S243 (= S246), S245 (= S248), K246 (= K249), R254 (= R257), R374 (= R376)
Query Sequence
>WP_057509011.1 NCBI__GCF_001431535.1:WP_057509011.1
MSFFANVELVPGDPILGLTEAYNADSRPTKVNLGVGIYYDESGRIPLLRAVKQIEQQLAT
EAKPRGYLPIDGLPAYTQATRELVFGKDSPLLAAGRVATTQTVGGSGALRVGAELLNKLL
PHATIAISNPSWENHRAVFGAAGFDVVEYSYFDAQTHGVDFPAMLADLQKLQPGTVVLLH
ACCHNPTGADLTVDQWKQVAEVLKEQQLFPFIDMAYQGFDKGIEQDGAAVRIIAEAGIDS
FVVANSYSKSFSLYGERVGALSIVAPNATEAKAVQSQIKRIIRTIYSSPSNHGAALVAGV
LNSPELRTLWEAELTEMRERIHALRHGLVDRLAALGAPEFAFINDQAGMFSYSGLSRVQV
EKLRDDYGIYAVGTGRICVAALSQSNLDYVAQAVANVAKA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory