SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_057509355.1 NCBI__GCF_001431535.1:WP_057509355.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 18 hits to proteins with known functional sites (download)

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
41% identity, 97% coverage: 12:459/460 of query aligns to 4:455/469 of P46349

query
sites
P46349

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G33 (= G41) mutation to D: Lack of activity.
G42 (= G50) mutation to S: Lack of activity.
G301 (≠ N308) mutation to V: Lack of activity.
G338 (≠ S345) mutation to E: Lack of activity.
F341 (= F348) mutation to S: Lack of activity.
G414 (≠ L420) mutation to R: Lack of activity.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
37% identity, 98% coverage: 7:458/460 of query aligns to 10:458/458 of P24207

query
sites
P24207

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R26 (= R23) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P51) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F84) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ Y87) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ A89) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (≠ A91) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ L92) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ T95) mutation to L: No effect on phenylalanine transport activity.
F101 (≠ A98) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W102) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (≠ W104) mutation to L: No effect on phenylalanine transport activity.
W108 (= W105) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ L108) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (= E115) mutation E->G,L,V,N: Loss of activity.
K168 (= K165) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E224) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R250) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P338) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.
P442 (≠ T442) mutation to A: 46% of wild-type phenylalanine transport activity.; mutation to G: 52% of wild-type phenylalanine transport activity.; mutation to L: 43% of wild-type phenylalanine transport activity.

Q88CZ8 L-histidine transporter HutT from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
38% identity, 93% coverage: 16:445/460 of query aligns to 7:441/467 of Q88CZ8

query
sites
Q88CZ8

L

L

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S

A

A

L

A

L

I

S

S

A

A

L

I

N

N

A

S

N

D

L

I

Y

F

G

G

A

A

S

G

R

R

M

M

I

M

F

Y

S

G

L

L

A

A

Q

Q

R

Q

R

G

E

H

A

A

P

P

A

R

V

G

L

F

G

S

W

K

A

L

D

S

R

K

R

H

Q

G

V

V

P

P

V

W

L

M

A

T

V

V

L

V

-

V

-

M

-

G

A

A

S

A

V

L

L

L

F

I

G

G

F

-

A

-

T

V

V

L

M

L

E

N

L

Y

V

L

F

I

P

P

D

E

R

N

V

V

L

F

P

L

V

L

L

I

L

A

N

S

I

I

V

A

G

T

S

F

T

A

C

T

L

V

L

W

V

V

W

W

T

L

L

M

S

I

L

L

S

T

Q

Q

L

V

V

A

L

M

R

R

R

S

A

M

D

S

R

R

A

E

G

Q

V

V

A

A

-

Q

L

L

P

K

F

F

R

P

M

V

A

P

A

F

F

W

P

P

W

Y

L

G

T

P

A

A

L

M

A

A

L

I

A

A

I

F

L

M

A

V

L

F

I

I

F

F

A

G

L

V

L

L

L

G

Y

Y

G

F

D

P

Q

D

T

T

R

Q

L

A

Q

A

F

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L

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S

V

M

G

V

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V

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L

W

T

V

L

V

F

F

I

L

T27 (≠ A36) mutation T->A,S: Retains 60% of wild-type activity.; mutation to N: Retains 20% of wild-type activity.
E98 (≠ Q107) mutation to A: Retains 80% of wild-type activity.
K156 (= K165) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
F212 (= F218) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
E218 (= E224) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
36% identity, 90% coverage: 15:426/460 of query aligns to 10:421/457 of P15993

query
sites
P15993

R

Q

L

L

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H

R

A

G

L

L

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K

P

N

R

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A

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A

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F

A

A

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D

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A

Y

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G

G

P

S

T

F

A

A

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G

A

F

T

A

V

S

G

G

W

W

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N

W

Y

W

W

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L

L
x
Y

V

V

I

L

V

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A

A

M

A

A

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A

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A

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A

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A

A

L

A

A

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F

F

M

F

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F

V

F

I

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N

A

A

I

I

N

N

L

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G

N

V

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K

N

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M

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F

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A

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F

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G

A

-

L

-

G

G

W

W

L

L

-

L

-

F

-

S

-

G

-

N

-

G

P

P

D

Q

A

A

T

T

S

-

P

-

G

-

L

V

S

S

N

N

F

L

T

W

G

D

N

Q

G

G

F

F

A

L

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P

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H

G

G

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F

A

T

G

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E

I

L

V

V

A

G

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I

A

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A

A

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E

T

A

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D

N

P

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A

A

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A

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A

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A

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A

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P

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D

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N

A

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F

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F

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M

A

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N

A

W

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A

A

L

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I

V

F

L

A

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L

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L

M

L

L

Y103 (≠ L108) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
34% identity, 89% coverage: 14:424/460 of query aligns to 11:433/489 of P25737

query
sites
P25737

P

P

R

G

L

L

G

R

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R

A

E

L

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K

P

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G

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Y

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A

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P

E

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G

A

F

G

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F

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x
Y

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Q

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x
W

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A

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D

A

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A

A

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A

F

F

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-

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N

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F

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K
|
K

V

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F

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I

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D

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A

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F

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I

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F

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E

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E

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D

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D

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A

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N

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C

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A

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D

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A

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-

D

-

I

-

N

A

D

L

L

P

P

F

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R

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A

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A

F

F

F

P

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-

L

L

T

G

A

P

L

I

A

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L

A

A

F

I

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L

A

C

L

L

I

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F

I

A

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L

L

Y102 (≠ W104) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (≠ L108) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K165) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F218) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E224) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E232) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 88% coverage: 15:421/460 of query aligns to 83:509/590 of P04817

query
sites
P04817

R

E

L

V

G

K

H

R

A

E

L

L

K

K

P

Q

R

R

Q

H

L

I

I

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M

M

I

G

A

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L

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I

I

G

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G

G

L

L

F

F

L

I

G

G

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L

G

S

V

T

G
x
P

V

L

Q

T

L

N

A

A

G

G

P

P

-

V

A

G

V

A

L

L

L

I

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S

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Y

L

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F

A

M

G

G

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A

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M

A

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K

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P
|
P

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x
V

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A
x
S

F

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Y

F

A

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D

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A

F

L

L

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A

A

F

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A

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N

G

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Y

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W
x
Y

W

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F

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A

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F

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-

S

-

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A

I

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N

N

L

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K

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V

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L

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x
G

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A

D

N

P
|
P

E

R

R

K

S

S

I

V

A

P

R

R

A

A

I

I

R

K

T

K

V

V

A

V

W

F

R

R

I

I

L

L

V

T

F

F

Y

Y

I

I

G

G

S

S

L

L

S

L

V

F

I

I

I

G

A

L

V

L

V

V

P

P

W

Y

-

N

-

D

-

P

-

K

-

L

-

T

-

Q

-

S

T
|
T

S
|
S

E
x
Y

A

V

L

S

K

T

S

S

P

P

F

F

A

I

V

A

L

I

E

E

A

N

A

S

N

G

I

T

P

K

G

V

A

L

G

P

T

H

A

I

I

F

T

N

L

A

V

V

A

I

V

L

I

T

A

T

L

I

S

S

A

A

L

A

N

N

A

S

N

N

L

I

Y

Y

G

V

A

G

S

S

R

R

M

I

I

L

F

F

S

G

L

L

A

S

Q

K

R

N

R

K

E

L

A

A

P

P

A

K

V

F

L

L

G

S

W

R

A

T

D

T

R

K

R

G

Q

G

V

V

P

P

V

Y

L

I

A

A

V

V

L

F

A

V

S

T

V

A

L

A

F

F

G

G

F

A

A

L

A

A

T

Y

V

M

M

E

E

T

L

S

V

T

F

G

P

G

D

D

R

K

V

V

L

F

P

E

V
x
W

L

L

N

N

I

I

V

T

G

G

S

V

T

A

C

G

L
x
F

L

F

V

A

W

W

T

L

L

F

S

I

L

S

L

I

S

S

Q

H

L

I

V

R

L

F

R

M

R

Q

R

A

A

L

D

K

R

Y

A

R

G

G

V

I

A

S

-

R

-

D

-

E

L

L

P

P

F

F

R

K

M

A

A

K

A

L

F

M

P

P

W

G

L

L

T

A

A

Y

L

Y

A

A

L

A

A

T

I

F

L

M

A

T

L

I

I

I

P113 (≠ G45) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (= P79) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (≠ T80) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (≠ A83) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (≠ W104) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (≠ A231) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P236) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (= TS 269:270) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (≠ E271) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ V366) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (≠ L376) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 86% coverage: 4:400/460 of query aligns to 68:476/587 of Q9URZ4

query
sites
Q9URZ4

N

K

P

P

T

A

P

K

A

A

A

T

P

G

A

E

S

D

P

G

P

T

R

A

L

L

G

K

H

R

A

S

L

L

K

K

P

S

R

R

Q

H

L

M

I

Q

M

M

M

I

G

S

L

I

G

G

S

G

A

A

I

I

G

G

A

T

G

G

L

L

F

Y

L

V

G

G

S

S

G

G

V

S

G

S

V

L

Q

A

L

D

A

G

G

G

P

P

A

A

-

S

V

V

L

I

S

N

Y

Y

L

S

V

L

A

I

G

G

A

I

L

M

V

M

I

F

V

I

M

V

N

Y

A

A

L

L

G

G

E

E

M

M

A

A

A

V

N

A

K

Y

P

P

T

V

S

A

G

G

A

G

F

F

S

N

V

T

Y

Y

A

A

A

T

D

R

A

F

L

I

G

D

P

P

T

A

A

W

G

G

A

F

T

A

V

V

G

S

W

W

L

N

W

Y

W

F

V

I

Q

N

L

Y

V

F

I

V

V

T

I

F

A

P

A

L

E

E

A

L

V

T

G

T

A

C

A

A

G

-

L

I

L

T

A

F

T

R

V

Y

W

W

T

T

G

D

L

I

S

N

V

S

P

A

M

A

A

W

A

I

L

S

A

I

F

F

M

L

L

V

F

V

F

I

T

I

A

I

I

V

N

N

L

L

L

F

G

G

V

V

K

R

N

A

F

Y

G

G

E

E

F

V

E

E

F

F

W

I

F

L

A

S

I

T

L

V

K

K

V

V

A

V

A

A

I

T

L

F

G

G

F

F

I

I

A

I

I

L

G

A

A

I

L

I

L

N

L

C

G

G

W

G

L

V

P

P

D

T

A

D

T

H

S

R

P

G

G

Y

L

I

S

G

N

G

F

S

T

I

G

I

N

K

G

H

G

K

F

P

A

F

P

R

T

H

G

G

L

F

A

K

G

G

V

F

G

C

A

S

A

V

L

F

L

T

V

T

V

A

I

A

F

F

A

S

F

F

G

S

G

G

T

T

E

E

I

V

V

I

A

G

V

L

A

A

E

E

T

V

E

D

D

N

P

P

E

Q

R

K

S

A

I

L

A

P

R

H

A

A

I

V

R

K

T

Q

V

V

A

F

W

W

R

R

I

I

L

A

V

I

F

F

Y

Y

I

V

G

V

S

S

L

L

S

I

V

L

I

I

I

G

A

L

V

L

V

I

P

S

W

P

T

D

S

D

E

P

A

N

L

L

K

M

-

G

-

N

-

G

-

S

-

T

-

S

-

V

S

S

P

P

F

F

A

V

A

L

V

A

L

I

E

K

A

E

A

A

N

N

I

I

P

K

G

G

A

L

G

P

T

S

A

V

I

F

T

N

L

A

V

V

A

I

V

I

I

I

A

S

L

V

S

S

A

V

L

T

N

N

A

S

N

S

L

T

Y

Y

G

T

A

A

S

G

R

R

M

T

I

L

F

H

S

G

L

M

A

A

Q

N

R

L

R

K

E

Q

A

A

P

P

A

S

V

F

L

F

G

K

W

Y

A

T

D

D

R

R

R

L

Q

G

V

R

P

P

V

L

L

L

A

A

V

M

L

I

A

V

S

V

V

L

L

L

F

F

G

G

F

F

A

F

A

A

T

Y

V

I

M

N

E

E

L

A

-

D

-

K

-

N

-

G

-

N

V

D

F

V

P

S

D

D

R

T

V

V

L

F

P

N

V

W

L

L

N

A

I

L

V

S

G

G

S

L

T

S

C

N

L

F

V

T

W

W

T

G

L

S

S

I

L

C

L

L

S

C

Q

H

L

I

V

I

L

F

R

R

R

L

R

A

A

F

D

K

R

K

A

Q

G

G

V

H

A

S

L

L

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
33% identity, 74% coverage: 16:354/460 of query aligns to 86:439/602 of P19145

query
sites
P19145

L

L

G

K

H

H

A

H

L

L

K

K

P

N

R

R

Q

H

L

L

I

Q

M

M

M

I

G

A

L

I

G

G

S

G

A

A

I

I

G

G

A

T

G

G

L

L

F

L

L

V

G

G

S

S

G

G

V

T

G

A

V

L

Q

R

L

T

A

G

G

G

P

P

A

A

V

S

L

L

-

I

S

G

Y

W

L

G

V

S

A

T

G

G

A

T

L

M

V

I

I

Y

I

A

V

M

M

V

N

M

A

A

L

L

G

G

E

E

M

L

A

A

A

V

N

I

K

F

P

P

T

I

S

S

G

G

A

G

F

F

S

T

V

T

Y

Y

A

A

A

T

D

R

A

F

L

I

G

D

P

E

T

S

A

F

G

G

A

Y

T

A

V

N

G

N

W

F

L

N

W

Y

W

M

V

L

Q

Q

L

W

V

L

I

V

V

V

I

L

A

P

A

L

E

E

A

I

V

V

G

S

A

A

A

S

G

-

L

I

L

T

A

V

T

N

V

F

W

W

T

G

G

-

L

-

S

T

V

D

P

P

M

K

A

Y

A

R

L

D

A

G

F

F

M

V

L

A

F

L

F

F

-

W

-

L

-

A

-

I

T

V

A

I

I

I

N

N

L

M

L

F

G

G

V

V

K

K

N

G

F

Y

G

G

E

E

F

A

E

E

F

F

W

V

F

F

A

S

I

F

L

I

K

K

V

V

A

I

A

T

I

V

L

V

G

G

F

F

I

I

A

I

I

L

G

G

A

I

L

L

L

N

L

C

G

G

W

G

L

G

P

P

D

T

A

G

T

G

S

Y

P

I

G

G

L

G

S

K

N

Y

F

W

T

H

G

D

N

P

G

G

G

A

F

F

A

A

P

G

T

D

-

T

-

P

-

G

-

A

G

K

L

F

A

K

G

G

V

V

G

C

A

S

A

V

L

F

L

V

V

T

V

A

I

A

F

F

A

S

F

F

G
x
A

G

G

T

S

E

E

I

L

V

V

A

G

V

L

A

A

A

S

E

E

T

S

E

V

D

E

P

P

E

R

R

K

S

S

I

V

A

P

R

K

A

A

I

A

R

K

T

Q

V

V

A

F

W

W

R

R

I

I

L

T

V

L

F

F

Y

Y

I

I

G

L

S

S

L

L

S

L

V

M

I

I

I

G

A

L

V

L

V

V

P

P

W

Y

T

N

S

D

E

K

A

S

L

L

-

I

-

G

-

A

-

S

-

V

-

D

-

A

K

A

S

S

P

P

F

F

A

V

A

I

V

A

L

I

E

K

A

T

A

H

N

G

I

I

P

K

G

G

A

L

G

P

T

S

A

V

I

V

T

N

L

V

V

V

A

I

V

L

I

I

A

A

L

V

L

L

S

S

A

V

L

G

N

N

A

S

N

A

L

I

Y

Y

G

A

A

C

S

S

R

R

M

T

I

M

F

V

S

A

L

L

A

A

Q

E

R

Q

R

R

E

F

A

L

P

P

A

E

V

I

L

F

G

S

W

Y

A

V

D

D

R

R

R

K

Q

G

V

R

P

P

V

L

L

V

A

G

V

I

L

A

A

V

S

T

V

S

L

A

F

F

G

G

F

L

A

I

A

A

T

F

V

V

A297 (≠ G221) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 88% coverage: 15:421/460 of query aligns to 144:570/663 of P48813

query
sites
P48813

R

N

L

L

G

K

H

K

A

S

L

I

K

K

P

P

R

R

Q

H

L

T

I

V

M

M

G

S

L

L

G

G

S

T

A

G

I

I

G

G

A

T

G

G

L

L

F

L

L

V

G

G

S

N

G

S

V

K

G

V

V

L

Q

N

L

N

A

A

G

G

P

P

A

G

-

G

V

L

L

I

S

G

Y

Y

L

A

V

I

A

M

G

G

A

S

L

C

V

V

I

Y

I

C

V

I

M

I

N

Q

A

A

L

C

G

G

E

E

M

L

A

A

A

V

-

I

N

Y

K

S

P

D

T

L

S

I

G

G

A

G

F

F

S

N

V

T

Y

Y

A

P

A

L

D

F

A

L

L

V

G

D

P

P

T

A

A

L

G

G

A

F

T

S

V

V

G

A

W

W

L

L

W

F

W

C

V

L

Q

Q

L

W

V

L

I

C

V

V

I

C

A

P

A

L

E

E

A

L

V

V

G

T

A

A

A

S

G

M

L

T

L

I

A

K

T

Y

V

W

W

T

T

T

G

S

L

V

S

N

V

P

P

D

M

V

A

F

A

V

L

V

A

I

F

F

M

Y

L

V

F

L

F

I

T

V

A

V

I

I

N

N

L

V

L

F

G

G

V

A

K

K

N

G

F

Y

G

A

E

E

F

A

E

D

F

F

W

F

F

F

A

N

I

C

L

C

K

K

V

I

A

L

A

M

I

I

L

V

G

G

F

F

I

-

A

-

I

-

G

-

A

-

L

F

L

I

L

L

G

A

W

I

L

I

P

I

D

D

A

C

T

G

S

G

P

A

G

G

L

T

S

D

N

G

F

Y

T

I

G

G

N

S

G

K

G

Y

F

W

A

R

P

D

T

P

G

G

-

A

-

F

-

R

-

G

-

D

-

T

-

P

-

I

-

Q

-

R

L

F

A

K

G

G

V

V

G

V

A

A

A

T

L

F

L

V

V

T

V

A

I

A

F

F

A

A

F

F

G

G

G

M

T

S

E

E

I

Q

V

L

A

A

V

M

A

T

A

A

A

S

E

E

T

Q

E

S

D

N

P

P

E

R

R

K

S

A

I

I

A

P

R

S

A

A

I

A

R

K

T

K

V

M

A

I

W

Y

R

R

I

I

L

L

V

F

F

V

Y

F

I

L

G

A

S

S

L

L

S

T

V

L

I

V

I

G

A

F

V

L

V

V

P

P

W

Y

T

T

S

S

E

D

A

Q

L

L

-

L

-

G

-

A

-

G

-

S

-

A

-

T

-

K

K

A

S

S

P

P

F

Y

A

V

A

I

V

A

L

V

E

S

A

S

A

H

N

G

I

V

P

R

G

V

A

V

G

P

T

H

A

F

I

I

T

N

L

A

V

V

A

I

V

L

I

L

A

S

L

V

L

L

S

S

A

V

L

A

N

N

A

G

N

A

L

F

Y

Y

G

T

A

S

S

S

R

R

M

I

I

L

F

M

S

S

L

L

A

A

Q

K

R

Q

R

G

E

N

A

A

P

P

A

K

V

C

L

F

G

D

W

Y

A

I

D

D

R

R

R

E

Q

G

V

R

P

P

V

A

L

A

A

A

V

M

L

L

A

V

S

S

V

A

L

L

F

F

G

G

F

V

A

I

A

A

T

F

V

C

M

A

E

S

L

S

V

K

F

K

P

E

D

E

R

D

V

V

L

F

P

T

V

W

L

L

N

A

I

I

V

S

G

G

S

L

T

S

C

Q

L

L

L

F

V

T

W

W

T

I

L

T

S

I

L

C

L

L

S

S

Q

H

L

I

V

R

L

F

R

R

R

A

A

M

D

K

R

V

A

Q

G

G

V

R

A

S

L

L

-

G

-

E

-

V

P

G

F

Y

R

K

M

S

A

Q

A

V

F

G

P

V

W

W

L

G

T

S

A

A

L

Y

A

A

-

V

-

L

L

M

A

M

I

V

L

L

A

A

L

L

I

I

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 90% coverage: 15:427/460 of query aligns to 276:772/852 of Q03770

query
sites
Q03770

R

H

L

I

G

Q

H

R

A

K

L

L

K

K

P

V

R

R

Q

H

L

I

I

Q

M

M

M

L

G

S

L

I

G

G

S

A

A

C

I

F

G

S

A

V

G

G

L

L

F

F

L

L

G

T

S

S

G

G

V

K

G

A

V

F

Q

S

L

I

A

A

G

G

P

P

-

F

A

G

V

T

L

L

S

G

Y

F

L

G

V

L

A

T

G

G

A

S

L

I

V

I

I

L

I

A

V

T

M

M

N

L

A

S

L

F

G

T

E

E

M

L

A

S

A

T

N

L

K

I

P

P

T

V

S

S

G

S

A

G

F

F

S

S

V

G

Y

L

A

A

A

S

D

R

A

F

L

V

G

E

P

D

T

A

A

F

G

G

A

F

T

A

V

L

G

G

W

W

L

T

W

Y

W

W

V

I

Q

S

L

C

V

M

I

L

V

A

I

L

A

P

A

A

E

Q

A

-

V

V

G

S

A

S

G
x
T

L

F

L

Y

A

L

T

S

V

Y

W

Y

T

N

G

N

L

V

S

N

V

I

P

S

M

K

A

G

A

V

L

T

A

A

-

G

F

F

M

I

L

T

F

L

F

F

T

S

A

A

-

F

-

S

-

I

-

V

I

V

N

N

L

L

G

D

V

V

K

S

N

I

F

M

G

G

E

E

F

I

E

V

F

Y

W

V

F

A

A

G

I

I

L

S

K

K

V

V

-

I

A

A

A

I

I

L

L

M

G

V

F

F

I

T

A

M

I

I

-

I

-

L

-

N

-

A

-

G

-

H

G

G

A

N

A

D

L

I

L

H

L

E

G

G

-

V

-

G

-

F

-

R

-

Y

W

W

L

-

P

-

D

-

A

D

T

S

S

S

P

K

G

S

L

V

S

R

N

N

F

L

T

T

G

-

N

Y

G

G

G

L

F

Y

A

R

P

P

T

T

-

F

-

D

-

L

-

A

-

D

-

A

-

G

-

E

-

G

-

S

-

K

G

G

L

I

A

S

G

G

-

P

-

K

-

G

-

R

-

F

-

L

-

A

V

T

G

A

A

S

A

V

L

M

L

L

V

I

V

S

I

T

F

F

A

A

F

F

G

S

G

G

T

V

E

E

I

M

V

T

A

F

V

L

A

A

A

S

A

G

E

E

T

A

E

I

D

N

P

P

E

R

R

K

S

T

I

I

A

P

R

S

A

A

I

T

R

K

T

R

-

T

-

F

-

S

-

I

-

V

-

L

V

I

A

S

W

Y

R

V

I

F

L

L

V

I

F

F

Y

S

I

V

G

G

S

-

L

I

S

N

V

I

-

Y

-

S

-

G

-

D

-

P

-

R

I

L

A

S

V

Y

V

F

P

P

W

G

T

I

S

S

E

E

A

K

L

-

K

-

S

-

P

R

F

Y

A

E

A

A

V

I

L

I

E

K

A

G

-

T

-

G

-

M

-

D

-

W

-

R

-

L

-

R

A

T

N

N

I

C

P

R

G

G

A

G

-

I

-

D

-

Y

-

R

-

Q

-

I

-

S

-

V

G

G

T

T

A

G

I

Y

T

S

L

S

V

P

A

W

V

V

I

V

A

A

L

L

L

Q

-

N

-

F

-

G

-

L

-

C

-

T

-

F

-

A

S

S

A

A

L

F

N

N

A

A

-

I

-

L

-

I

-

F

-

T

-

A

-

T

-

A

-

G

-

I

-

S

N

S

L

L

Y

F

G

S

A

C

S

S

R

R

M

T

I

L

F

Y

S

A

L

M

A

S

Q

V

R

Q

R

R

E

K

A

A

P

P

A

P

V

V

L

F

G

E

W

I

A

C

D

S

R

K

R

R

Q

G

V

V

P

P

V

Y

L

V

A

S

V

V

L

I

A

F

S

S

V

S

L

L

F

F

G

S

F

V

A

I

A

A

T

Y

V

I

M

A

E

V

L

D

V

Q

F

T

P

A

D

I

R

E

V

N

L

F

P

D

V

V

L

L

L

A

N

N

I

V

V

S

G

S

S

A

T

S

C

T

L

S

L

I

V

I

W

W

-

M

-

G

-

L

T

N

L

L

S

S

L

F

L

L

S

R

-

F

Q

Y

L

Y

V

A

L

L

R

K

R

Q

R

R

A

K

D

D

-

I

-

S

R

R

A

N

G

D

V

S

A

S

L

Y

P

P

F

Y

R

K

M

S

A

P

A

F

F

Q

P

P

W

Y

L

L

T

A

A

I

L

Y

A

G

L

L

A

V

I

G

L

C

A

S

L

L

I

F

F

V

A

I

L

F

L

M

L

G

Y

Y

T382 (≠ G121) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
25% identity, 85% coverage: 15:406/460 of query aligns to 27:445/629 of P30825

query
sites
P30825

R

R

L

L

G

S

H

R

A

C

L

L

K

N

P

T

R

F

Q

D

L

L

I

V

M

A

M

L

G

G

L

V

G

G

S

S

A

T

I

L

G

G

A

A

G

G

L

V

F

Y

L

V

G

L

S

A

G

G

-

A

V

V

G

A

V

R

Q

E

L

N

A

A

G

G

P

P

A

A

V

I

L

V

L

I

S

S

Y

F

L

L

V

I

A

A

G

A

A

L

L

A

V

S

I

V

I

L

V

A

M

G

N

L

A

C

L

Y

G

G

E

E

M

F

A

G

A

A

N

R

K

V

P

P

T

K

S

T

G

G

A

S

F

A

S

Y

V

L

Y

Y

A

S

A

Y

D

V

A

T

L

V

G

G

P

E

T

L

A

W

G

A

A

F

T

I

V

T

G

G

W

W

L

N

W

L

W

I

V

L

Q

S

L

Y

V

I

I

I

V

G

I

T

A

S

-

V

-

A

-

R

-

A

-

W

-

S

-

A

-

T

-

F

-

D

E

E

A

L

V

I

G

G

A

R

A

P

-

I

G

G

L

E

L

F

A

S

T

R

V

T

W

H

T

M

G

T

L

L

S

N

V

A

P

P

-

G

-

V

-

L

-

A

-

E

-

N

-

P

-

D

M

I

A

F

A

A

L

V

A

I

F

I

M

I

L

L

F

I

F

L

T

T

A

G

I

L

N

L

L

T

L

L

G

G

V

V

K

K

N

E

F

S

G

A

E

M

F

V

E

N

F

K

W

I

F

F

A

T

I

C

L

I

K

N

V

V

A

L

A

-

I

V

L

L

G

G

F

F

I

I

A

M

I

V

G

S

A

G

A

F

L

V

-

K

-

G

-

S

L

V

L

K

G

N

W

W

L

Q

P

L

D

T

A

E

T

E

S

D

P

F

G

G

-
x
N

-

T

-

S

-

G

-

R

-

L

-

C

L

L

S

N

N

N

F

D

T

T

-

K

-

E

-

G

-

K

-

P

G

G

N

V

G

G

F

F

A

M

P

P

T

F

G

G

L

F

A

S

G

G

V

V

G

L

A

S

A

G

L

A

V

T

V

C

I

F

F

Y

A

A

F

F

G

V

G

G

T

F

E

D

I

C

V

I

A

A

V

T

A

T

A

G

A

E

E

E

T

V

E

K

D

N

P

P

E

Q

R

K

S

A

I

I

A

P

R

V

A

G

I

I

R

V

T

A

V

S

A

L

W

L

R

I

I

C

L

F

V

I

F

A

Y

Y

I

F

G

G

S

V

L

S

S

A

V

A

I

L

I

T

A

L

V

M

P

P

W

Y

T

F

S

C

E

L

A

D

L

N

K

N

S

S

P

P

F

L

A

P

A

D

V

A

L

F

E

K

A

H

A

V

N

G

I

W

P

E

G

G

A

A

G

K

T

Y

A

A

I

V

T

A

L

V

V

G

A

S

V

L

I

C

A

A

L

L

L

S

S

A

A

S

L

L

N

L

A

G

N

S

L

M

Y

F

G

P

A

M

S

P

R

R

M

V

I

I

F

Y

S

A

L

M

A

A

Q

E

R

D

R

G

E

L

A

L

P

F

A

K

V

F

L

L

-

A

G

N

W

V

A

N

D

D

R

R

R

T

Q

K

V

T

P

P

V

I

L

I

A

A

V

T

L

L

A

A

S

S

V

-

L

-

F

-

G

G

F

A

A

V

A

A

T

A

V

V

M

M

E

A

L

F

V

L

F

F

P

D

D

-

R

-

V

-

L

L

P

K

V

D

L

L

L

V

N

D

I

L

V

M

G

S

S

I

T

G

C

T

L

L

V

A

W

Y

T

S

L

L

S

V

L

A

S

C

Q

V

L

L

V

V

L

L

R

R

R

Y

R

Q

A

P

D

E

R

Q

A

P

G

N

V

-

A

-

L

L

P

V

F

Y

R

Q

M

M

A

A

A

S

N226 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
25% identity, 70% coverage: 47:370/460 of query aligns to 55:378/458 of 6f34A

query
sites
6f34A

Q

E

L

H

A

A

G

G

P

P

A

A

V

L

L

V

L

L

S

S

Y

F

L

I

V

L

A

S

G

G

A

L

L

A

V

C

I

V

I

F

V

A

M

A

N

L

A

C

L

Y

G

A

E

E

M

F

A

A

A

S

N

T

K

V

P

P

T

V

S

S

G

G

A

S

F

A

S

Y

V

T

Y

Y

A

S

A

Y

D

A

A

T

L

F

G

G

P

E

T

L

A

I

G

A

A

W

T

I

V

L

G

G

W

W

L

D

W

L

W

I

V

L

Q
x
E

L
x
Y

V

G

I

V

V
x
A

I

S

A

S

A

A

E

V

A

A

V

V

G

G

A

W

A

S

G

G

L

Y

L

F

A

Q

T

G

V

L

W

L

T

S

G

G

-

E

-

L

-

P

-

K

-

A

-

L

-

T

-

S

-

A

-

Y

-

D

-

P

-

A

-

K

-

G

L

T

S

F

V

I

P

D

M

L

A

P

A

A

L

I

A

I

F

I

M

V

L

L

F

F

F

I

T

T

A

F

I

L

N

L

L

N

L

L

G

G

V

A

K

K

N

K

F

S

G
x
A

E
x
R

F

F

E

N

F

A

W

V

F

I

A

V

I
x
A

L
x
I

K

K

V

V

A
|
A

A

V

I

V

L

L

G
x
L

F

F

I

L

A

A

I

V

G

G

A

V

A

-

L

-

G

-

W
|
W

L
x
Y

-

V

-

K

P

P

D

E

A

N

T

W

S

T

P

P

G

-

L

-

S

-

N

-

F

-

T

-

G

-

N

-

G

-

F

F

A

M

P

P

T

Y

G

G

L

F

A

S

G

G

V

V

G

A

A

T

A

G

L

A

V

T

V

V

I

F

F
|
F

A
|
A

F

Y

G
x
I

G

G

T

F

E

D

I

A

V

V

A

S

V

T

A

A

A

E

E

E

T

V

E

R

D

N

P

P

E

Q

R

R

S

D

I

M

A

P

R

I

A

G

I

I

R

I

T

V

V

S

A

L

W

L

R

V

I

C

L

T

V

L

F

L

Y

Y

I

I

G

A

S

V

L

S

S

L

V

V

I

L

I

T

A

G

V

I

V

V

P

P

W

Y

T

E

S

Q

E

L

A

N

L

V

K

K

S

N

P

P

F

V

A

A

A

F

V

A

L

L

E

N

A

Y

A

I

N

H

I
x
Q

P

D

G

W

A
x
V

G

A

T

G

A

F

I

I

T

S

L

L

V

G

A

A

V

I

I

A

A

G

L

I

L

T

S

T

A
x
V

L

L

N

L

A

V

N

S

L

M

Y

Y

G

G

A

Q

S

T

R

R

M

L

I

F

F

Y

S

A

L

I

A

S

Q

R

R

D

R

G

E

L

A

L

P

P

A

K

V

V

L

F

G

A

W

R

-

I

A

S

D

P

R

T

R

R

Q

Q

V

V

P

P

V

-

L

-

A

-

V

Y

L

V

A

N

S

T

V

W

L

L

F

T

G

G

F

A

A

A

A

V

T

A

V

V

M

F

E

A

L

G

V

I

F

I

P

P

D

L

R

N

V

K

L

L

P

A

V

E

L

L

L

T

N

N

I

I

binding arginine: E115 (≠ Q107), Y116 (≠ L108), A119 (≠ V111), F228 (= F218), A229 (= A219), I231 (≠ G221), V314 (≠ A304)
binding cholesterol: W201 (= W183), Y202 (≠ L184)
binding : A178 (≠ G155), R179 (≠ E156), A186 (≠ I163), I187 (≠ L164), A190 (= A167), L194 (≠ G171), Q296 (≠ I286), V299 (≠ A289)

Sites not aligning to the query:

binding arginine: 40, 42, 43, 44
binding : 28, 30, 31, 34

5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
26% identity, 75% coverage: 47:391/460 of query aligns to 53:394/456 of 5oqtA

query
sites
5oqtA

Q

E

L

H

A

A

G

G

P

P

A

A

V

L

L

V

L

L

S

S

Y

F

L

I

V

L

A

S

G

G

A

L

L

A

V

C

I

V

I

F

V

A

M

A

N

L

A

C

L

Y

G

A

E

E

M

F

A

A

A

S

N

T

K

V

P

P

T

V

S

S

G

G

A

S

F

A

S

Y

V

T

Y

Y

A

S

A

Y

D

A

A

T

L

F

G

G

P

E

T

L

A

I

G

A

A

W

T

I

V

L

G

G

W

W

L

D

W

L

W

I

V

L

Q

E

L

Y

V

G

I

V

V

A

I

S

A

S

A

A

E

V

A

A

V

V

G

G

A

W

A

S

G

G

L

Y

L

F

A

Q

T

G

V

L

W

L

T

S

G

G

-

E

-

L

-

P

-

K

-

A

-

L

-

T

-

S

-

A

-

Y

-

D

-

P

-

A

-

K

-

G

L

T

S

F

V

I

P

D

M

L

A

P

A

A

L

I

A

I

F

I

M

V

L

L

F

F

F

I

T

T

A

F

I

L

N

L

L

N

L

L

G

G

V

A

K

K

N

K

F

S

G
x
A

E
x
R

F

F

E

N

F

A

W

V

F

I

A

V

I
x
A

L

I

K

K

V

V

A
|
A

A

V

I

V

L

L

G
x
L

F

F

I

L

A

A

I

V

G

G

A

V

A

-

L

-

G

-

W

W

L

Y

-

V

-

K

P

P

D

E

A

N

T

W

S

T

P

P

G

-

L

-

S

-

N

-

F

-

T

-

G

-

N

-

G

-

F

F

A

M

P

P

T

Y

G

G

L

F

A

S

G

G

V

V

G

A

A

T

A

G

L

A

V

T

V

V

I

F

F
|
F

A
|
A

F

Y

G
x
I

G

G

T

F

E

D

I

A

V

V

A

S

V

T

A

A

A

E

E

E

T

V

E

R

D

N

P

P

E

Q

R

R

S

D

I

M

A

P

R

I

A

G

I

I

R

I

T

V

V

S

A

L

W

L

R

V

I

C

L

T

V

L

F

L

Y

Y

I

I

G

A

S

V

L

S

S

L

V

V

I

L

I

T

A

G

V

I

V

V

P

P

W

Y

T

E

S

Q

E

L

A

N

L

V

K

K

S

N

P

P

F

V

A

A

A

F

V

A

L

L

E

N

A

Y

A

I

N

H

I
x
Q

P

D

G

W

A
x
V

G

A

T

G

A

F

I

I

T

S

L

L

V

G

A

A

V

I

I

A

A

G

L

I

L

T

S

T

A

V

L

L

N

L

A

V

N

M

L

M

Y

Y

G

G

A

Q

S

T

R

R

M

L

I

F

F

Y

S

A

L

I

A

S

Q

R

R

D

R

G

E

L

A

L

P

P

A

K

V

V

L

F

G

A

W

R

-

I

A

S

D

P

R

T

R

R

Q

Q

V

V

P

P

V

-

L

-

A

-

V

Y

L

V

A

N

S

T

V

W

L

L

F

T

G

G

F

A

A

A

A

V

T

A

V

V

M

F

E

A

L

G

V

I

F

I

P

P

D

L

R

N

V

K

L

L

P

A

V

E

L

L

L

T

N

N

I

I

V

-

G

-

S

G

T

T

C

L

L

F

L

A

V

F

W

I

T

T

L

V

S

S

L

-

L

I

S

G

Q

V

L

L

V

V

L

L

R

R

R

K

binding alanine: F226 (= F218), A227 (= A219), I229 (≠ G221)
binding : A176 (≠ G155), R177 (≠ E156), A184 (≠ I163), A188 (= A167), L192 (≠ G171), Q294 (≠ I286), V297 (≠ A289)

Sites not aligning to the query:

binding alanine: 38, 40, 41, 42
binding : 24, 26, 28, 29, 32

O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
22% identity, 84% coverage: 30:416/460 of query aligns to 22:398/438 of O34739

query
sites
O34739

L

I

G

G

S

T

A

I

I

I

G

G

A

S

G

G

L

V

F

F

L

M

G

K

S

P

G

G

V

A

G

V

V

L

Q

A

L

Y

A

S

G

G

P

D

A

S

-

K

-

M

V

A

L

L

L

F

S

A

Y

W

L

L

V

L

A

G

G

G

A

I

L

L

V

T

I

L

I

A

V

G

M

G

N

L

A

T

L

I

G

A

E

E

M

I

A

G

A

T

N

Q

K

I

P

P

T

K

S

T

G

G

A

G

F

L

S

Y

V

T

Y

Y

A

L

A

E

D

E

A

V

L

Y

G

G

P

E

T

F

A

W

G

G

A

F

T

L

V
x
C

G

G

W

W

L

-

W

-

V

V

Q

Q

L

I

V

I

I

I

-

Y

-

G

-

P

-

A

V

I

I

I

A

G

A

A

E

L

A

G

V

L

G

Y

A

F

A

G

G

S

L

L

L

M

A

A

T

N

V

L

W

F

-

G

-

W

-

G

T

S

G

G

L

L

S

S

V

K

P

V

M

I

A

G

A

I

L

I

A

A

F

-

M

V

L

L

F

F

F

L

T
x
C

A

V

I

I

N

N

L

I

G

G

V

T

K

K

N

Y

F

G

G

G

E

F

F

V

E

Q

F

T

W

L

F

T

A

T

I

I

L

G

K

K

V

L

A

I

A

P

I

I

L

A

G
x
C

F

I

I

I

A

V

I

F

G

G

A

L

A

-

L

-

G

-

W

W

L

K

P

G

D

D

-

Q

-

H

-

I

-

F

-

T

A

A

T

V

S

N

P

E

G

S

L

I

S

S

N

D

F

M

T

N

G

-

N

-

G

-

F

-

A

-

P

-

T

-

G

-

L

-

A

-

G

-

V

F

G

G

A

A

L

I

L

L

V

A

V

T

I

L

F

F

A

A

F

Y

G

D

G

G

T

W

E

I

I

L

V

L

A

A

V

A

A

L

A

G

E

E

T

M

E

K

D

N

P

P

E

E

R

K

S

L

I

L

A

P

R

R

A

A

I

M

R

T

T

G

V

G

A

L

W

L

R

I

I

V

L

T

V

A

F

I

Y

Y

I

I

G

F

S

I

L

N

S

F

V

A

I

L

A

H

V

I

V

L

P

S

W

A

T

N

S

E

E

I

A

V

L

T

K

L

S

G

P

E

F

N

A

A

A

T

V

S

L

T

E

A

A

A

A

T

N

M

I

L

P

F

G

G

A

S

-

I

-

G

G

G

T

K

A

L

I

I

T

S

L

V

V

G

A

I

V

I

I

V

A

S

L

I

L

F

S

G

A
x
C

L

L

N

N

A

G

N

K

L

V

Y

L

G

S

A

F

S

P

R

R

M

V

I

S

F

F

S

A

L

M

A

A

Q

E

R

R

R

K

E

Q

A

L

P

P

A

F

V

A

L

E

G

K

W

L

A

S

D

H

R

V

R

H

-

P

-

S

-

F

Q

R

V

T

P

P

V

W

L

I

A

A

V

I

L

-

A

-

S

S

V

F

L

Q

F

I

G

A

F

L

A

A

A

L

T

I

V

M

M

M

E

L

L

I

V

S

F

N

P

P

D

D

R

K

V

L

L

S

P

E

V

I

L

S

L

I

N

F

I

M

V

I

G

-

S

-

T

-

C

-

L

Y

L

I

V

F

W

Y

T

V

L

M

S

A

L

F

S

A

Q

V

L

F

V

I

L

L

R

R

R

K

R

R

A

A

D

K

R

-

A

-

G

G

V

E

A

K

L

R

P

A

F

Y

R

S

M

V

A

P

A

L

F

Y

P

P

W

F

L

M

T

P

A

I

L

L

A

A

L

I

A

A

C94 (≠ V100) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
C141 (≠ T144) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
C168 (≠ G171) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
C291 (≠ A304) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.

Sites not aligning to the query:

415 C→S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.

6f2wA Bacterial asc transporter crystal structure in open to in conformation (see paper)
24% identity, 84% coverage: 30:415/460 of query aligns to 14:392/433 of 6f2wA

query
sites
6f2wA

L

V

G

G

S

T

A
x
V

I

I

G
|
G

A
|
A

G
|
G

L

I

F

F

L

F

G

K

S

P

G

T

V

A

G

V

V

Y

Q

G

L

A

A

A

G

G

-

A

P

P

A

G

V

L

-

G

L

L

S

A

Y

W

L

F

V

V

A

A

G

G

A

I

L

I

V

T

I

I

I

A

V

G

M

G

N

L

A

T

L

V

G

A

E

E

M

I

A

G

A

T

N

I

K

Y

P

P

T

Q

S

T

G

G

A

G

F

M

S

M

V

I

Y

Y

A

L

A

E

D

K

A

V

L

Y

G

G

P

R

T

W

A

L

G

G

A

F

T

L

V

V

G

G

W

W

L

-

W

-

V

A

Q

Q

L

M

V

V

I

I

V

Y

I

Y

A

P

A

A

E

N

A

I

V

A

G

A

A

L

A

A

G

I

L

I

L

F

A

A

T

T

V

Q

W

F

T

V

G

N

L

L

S

F

-

A

-

L

-

S

-

D

-

S

-

T

-

I

V

V

P

P

M

T

A

A

A

I

L

L

A

T

F

-

M

S

L

I

F

F

F

L

T

M

A

G

I

V

N

N

L

F

L

L

G

G

V

T

K

K

N

Y

F

S

G

G

E

W

F

I

E

Q

F

T

W

L

F

A

A

T

I

I

L

L

K

K

V

L

A

I

A

P

I

L

L

V

G

V

F

I

I

I

A

V

I

A

-

G

-

L

-

Y

-

P

-

G

G

G

A

G

A

V

L

I

L

R

L

L

G

V

W

P

L

F

P

S

D

V

A

E

T

T

S

H

P

P

G

V

L

L

S

T

N

S

F

F

T

-

G

-

N

-

G

-

F

-

A

-

P

-

T

-

G

-

L

-

A

-

G

-

V

-

G

G

A

S

A

A

L

L

L

I

V

A

V

T

I

L

F
|
F

A
|
A

F

Y

G
x
D

G

G

T

W

E

I

I

N

V

V

A

G

V

T

A

L

A

A

A

G

E

E

T

M

E

K

D

N

P

P

E

G

R

K

S

M

I

L

A

P

R

K

A

V

I

I

R

-

T

-

V

-

A

-

W

-

R

-

I

-

L

-

V

-

F

-

Y

-

I

I

G

G

S

G

L

L

S

S

V

I

I

V

I

M

A

A

V

V

V

Y

P

L

W

L

T

T

S

N

E

I

A

A

L

-

K

-

S

-

P

-

F

Y

A

L

A

F

V

V

L

L

E

D

A

S

N

Q

I

L

P

A

G

G

A

T

G

D

T

T

A

P

I

A

T

A

L

L

V

V

A

A

-

S

-

H

-

L

-

F

-

E

-

G

-

I

-

G

-

S

-

K

-

L

-

V

-

T

-

I

-

G

-

I

V

L

I

I

A

S

L

V

L

F

S

G

A

G

L

I

N

N

A

G

N

Y

L

I

Y

I

G

S

A

G

S

L

R

R

M

V

I

P

F

Y

S

A

L

L

A

A

Q

T

R

Q

R

K

E

M

A

L

P

P

A

F

V

S

L

D

G

W

W

F

A

A

D

R

R

I

R

N

Q

P

V

K

P

T

V

N

L

L

A

P

V

I

L

N

A

G

S

G

V

L

L

V

F

M

G

L

F

G

A

I

A

A

T

I

V

V

M

M

E

I

L

L

V

T

F

G

P

Q

D

F

R

N

V

Q

L

L

P

T

V

D

L

L

L

I

N

-

I

-

V

-

G

-

S

-

T

-

C

V

L

F

L

V

V

I

W

W

-

F

-

I

T

T

L

L

S

T

L

F

L

I

S

A

Q

V

L

I

V

I

L

L

R

R

R

K

R

-

A

-

D

T

R

Q

A

P

G

D

V

I

A

E

L

R

P

P

F

Y

R

R

M

V

A

P

A

F

F

Y

P

P

W

V

L

I

T

P

A

L

L

I

A

A

L

I

binding alpha-aminoisobutyric acid: V17 (≠ A33), G19 (= G35), A20 (= A36), G21 (= G37), F199 (= F218), A200 (= A219), D202 (≠ G221)

9h76A Bacterial lat transporter basc in complex with l-ala and nb53 (see paper)
24% identity, 84% coverage: 30:415/460 of query aligns to 12:390/430 of 9h76A

query
sites
9h76A

L

V

G

G

S

T

A

V

I

I

G
|
G

A
|
A

G
|
G

L

I

F

F

L

F

G

K

S

P

G

T

V

A

G

V

V

Y

Q

G

L

A

A

A

G

G

-

A

P

P

A

G

V

L

-

G

L

L

S

A

Y

W

L

F

V

V

A

A

G

G

A

I

L

I

V

T

I

I

I

A

V

G

M

G

N

L

A

T

L

V

G

A

E

E

M

I

A

G

A

T

N

I

K

Y

P

P

T

Q

S

T

G

G

A

G

F

M

S

M

V

I

Y

Y

A

L

A

E

D

K

A

V

L

Y

G

G

P

R

T

W

A

L

G

G

A

F

T

L

V

V

G

G

W

W

L

-

W

-

V

A

Q

Q

L

M

V

V

I

I

V

Y

I

Y

A

P

A

A

E

N

A

I

V

A

G

A

A

L

A

A

G

I

L

I

L

F

A

A

T

T

V

Q

W

F

T

V

G

N

L

L

S

F

-

A

-

L

-

S

-

D

-

S

-

T

-

I

V

V

P

P

M

T

A

A

A

I

L

L

A

T

F

-

M

S

L

I

F

F

F

L

T

M

A

G

I

V

N

N

L

F

L

L

G

G

V

T

K

K

N

Y

F

S

G

G

E

W

F

I

E

Q

F

T

W

L

F

A

A

T

I

I

L

L

K

K

V

L

A

I

A

P

I

L

L

V

G

V

F

I

I

I

A

V

I

A

-

G

-

L

-

Y

-

P

-

G

G

G

A

G

A

V

L

I

L

R

L

L

G

V

W

P

L

F

P

S

D

V

A

E

T

T

S

H

P

P

G

V

L

L

S

T

N

S

F

F

T

-

G

-

N

-

G

-

F

-

A

-

P

-

T

-

G

-

L

-

A

-

G

-

V

-

G

G

A

S

A

A

L

L

L

I

V

A

V

T

I

L

F

F

A

A

F

Y

G
x
D

G

G

T

W

E

I

I

N

V

V

A

G

V

T

A

L

A

A

A

G

E

E

T

M

E

K

D

N

P

P

E

G

R

K

S

M

I

L

A

P

R

K

A

V

I

I

R

-

T

-

V

-

A

-

W

-

R

-

I

-

L

-

V

-

F

-

Y

-

I

I

G

G

S

G

L

L

S

S

V

I

I

V

I

M

A

A

V

V

V

Y

P

L

W

L

T

T

S

N

E

I

A

A

L

-

K

-

S

-

P

-

F

Y

A

L

A

F

V

V

L

L

E

D

A

S

N

Q

I

L

P

A

G

G

A

T

G

D

T

T

A

P

I

A

T

A

L

L

V

V

A

A

-

S

-

H

-

L

-

F

-

E

-

G

-

I

-

G

-

S

-

K

-

L

-

V

-

T

-

I

-

G

-

I

V

L

I

I

A

S

L

V

L

F

S

G

A

G

L

I

N

N

A

G

N

Y

L

I

Y

I

G

S

A

G

S

L

R

R

M

V

I

P

F

Y

S

A

L

L

A

A

Q

T

R

Q

R

K

E

M

A

L

P

P

A

F

V

S

L

D

G

W

W

F

A

A

D

R

R

I

R

N

Q

P

V

K

P

T

V

N

L

L

A

P

V

I

L

N

A

G

S

G

V

L

L

V

F

M

G

L

F

G

A

I

A

A

T

I

V

V

M

M

E

I

L

L

V

T

F

G

P

Q

D

F

R

N

V

Q

L

L

P

T

V

D

L

L

L

I

N

-

I

-

V

-

G

-

S

-

T

-

C

V

L

F

L

V

V

I

W

W

-

F

-

I

T

T

L

L

S

T

L

F

L

I

S

A

Q

V

L

I

V

I

L

L

R

R

R

K

R

-

A

-

D

T

R

Q

A

P

G

D

V

I

A

E

L

R

P

P

F

Y

R

R

M

V

A

P

A

F

F

Y

P

P

W

V

L

I

T

P

A

L

L

I

A

A

L

I

binding alanine: G17 (= G35), A18 (= A36), G19 (= G37), D200 (≠ G221)

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
24% identity, 76% coverage: 8:358/460 of query aligns to 10:360/461 of P76037

query
sites
P76037

A

A

P

Q

A

P

S

G

P

K

P

T

R

R

L

L

G

R

H

K

A

S

L

L

K

K

P

L

R

W

Q

Q

L

V

I

V

M

M

G

G

L

L

G

A

S

Y

A

L

I

T

G

P

A

M

G

T

L

V

F

F

L

D

G

T

S

F

G

G

V

I

G

V

V

S

Q

G

L

I

A

S

G

D

P

G

A

H

V

V

L

P

L

A

S

S

Y

Y

L

L

V

L

A

A

G

L

A

A

L

G

V

V

I

L

I

F

V

T

M

A

N

I

A

S

L

Y

G

G

E

K

M

L

A

V

A

R

N

Q

K

F

P

P

T

E

S

A

G

G

A

S

F

A

S

Y

V

T

Y

Y

A

A

A

Q

D

K

A

S

L

I

G

N

P

P

T

H

A

V

G

G

A

F

T

M

V

V

G

G

W

W

L

S

W

S

W

L

V

L

Q

D

L
x
Y

V

L

I

F

V

L

I

P

A

M

A

I

E

N

A

V

V

L

G

L

A

A

A

K

G

I

L

Y

L

L

A

S

T

A

V

L

W

F

T

P

G

E

L

V

S

P

V

P

P

W

M

V

A

W

A

V

L

V

A

T

F

F

M

V

L

A

F

I

F

L

T

T

A

A

I

A

N

N

L

L

L

K

G

S

V

V

K

N

N

L

F

V

G

A

E

N

F

F

E

N

F

T

W

L

F

F

A

V

I

L

L

V

K

Q

V

I

A

S

A

I

I

M

L

V

G

V

F

F

I

I

A

F

I

L

G

V

A

V

A

Q

L

G

L

L

L

H

G

K

W

G

L

E

P

G

D

V

A

G

T

T

S

V

P

W

G

S

L

L

S

Q

N

P

F

F

T

I

G

S

N

E

G

N

G

A

F

-

A

-

P

-

T

-

G

H

L

L

A

I

G

P

V

I

G

I

A

T

A

G

L

A

L

T

V

I

V

V

I

C

F

F

A

S

F

F

G

L

G

G

T

F

E

D

I

A

V

V

A

T

V

T

A

L

A

S

A

E

E

E

T

T

E

P

D

D

P

A

E

A

R

R

S

V

I

I

A

P

R

K

A

A

I

I

R

F

T

L

V

T

A

A

W

V

R

Y

I

G

L

G

V

V

F

I

Y

F

I

I

G

A

S

A

L

S

S

F

V

F

I

M

I

Q

A

L

V

F

P

P

W

D

T

I

S

S

E

R

A

-

L

F

K

K

S

D

P

P

F

D

A

A

V

L

L

P

E

E

A

I

A

A

N

L

I

Y

P

V

G

G

A

G

G

K

T

L

A

F

I

Q

T

S

L

I

V

F

A

L

V

C

I

T

A

T

L

F

L

V

S

N

A

T

L

L

N

A

A

S

N

G

L

L

Y

A

G

S

A

H

-

A

-

S

-

V

S

S

R

R

M

L

I

L

F

Y

S

V

L

M

A

G

Q

R

R

D

R

N

E

V

A

F

P

P

A

E

-

R

V

V

L

F

G

G

W

Y

A

V

D

H

R

P

R

K

-

W

Q

R

V

T

P

P

V

A

L

L

A

N

V

V

L

I

A

M

S

V

V

G

L

I

F

V

G

A

F

L

A

S

A

A

T

L

V

F

M

F

E

D

L

L

V

V

Y110 (≠ L108) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

P0AAF1 Putrescine transporter PotE; Putrescine-proton symporter / putrescine-ornithine antiporter from Escherichia coli (strain K12) (see 2 papers)
23% identity, 68% coverage: 81:394/460 of query aligns to 69:379/439 of P0AAF1

query
sites
P0AAF1

S

S

G

G

A

G

F

M

S

G

V

G

Y

Y

A

A

A
x
E

D
x
Y

A

A

L

F

G

G

P
x
K

T

S

A

G

G

N

A

F

T

M

V

A

G

N

W
x
Y

L

T

W
x
Y

W

G

V

V

Q

S

L

L

V

L

I

I

V

A

I

N

A

V

A

A

E

I

A

A

V

I

G

S

A

A

A

V

G

G

-

Y

-

G

-

T

-

E

L

L

A

G

T

A

V

S

W

L

T

S

G

P

L

V

S

Q

V

I

P

G

M

L

A

A

A

T

L

I

A

G

F

V

M

L

L

W

F

I

F

C

T

T

A

V

I

A

N

N

L

F

L

G

G

G

V

A

K

R

N

I

F

T

G

G

E

Q

F

I

E

S

-

S

-

I

-

T

F

V

W

W

F

G

A

V

I

I

L

I

K

P

V

V

A

V

A

G

I

L

L

C

G

-

F

-

I

-

A

-

I

-

G

-

A

-

L

-

L

I

G

G

W

W

L

F

P

-

D

-

A

W

T

F

S

S

P

P

G

T

L

L

S

Y

N

V

F

D

T

S

G

W

N

N

G

P

G

H

F

H

A

A

P

P

T

F

G

-

L

F

A

S

G

A

V

V

G

G

A

S

L

I

L

A

V

M

V

T

I

L

F
x
W

A

A

F

F

G

L

G

G

T

L

E
|
E

I

S

V

A

A
x
C

V

A

A

N

A

T

A

D

E

V

T

V

E

E

D

N

P

P

E

E

R

R

S

N

I

V

A

P

R

I

A

A

I

V

R

L

T

G

V

G

A

T

W

L

R

G

I

A

L

A

V

V

F

I

Y

Y

I

I

G

V

S

S

L

T

S

N

V

V

I

I

I

A

A

G

V

I

V

V

P

P

W

N

T

M

S

E

E

L

A

A

L

N

K

S

S

T

-

A

P

P

F

F

A

G

A

L

V

A

L

F

E

A

A

Q

A

M

N

F

I

T

P

P

G

E

A

V

G

G

T

K

A

V

I

I

T

M

L

A

V

L

A

M

V

V

I

M

A

S

L
x
C

S

G

A

S

L

L

N

L

A

G

N
x
W

L

Q

Y

F

G

T

A

I

S

A

R

Q

M

V

I

F

F
x
K

S

S

L

S

A

S

Q

D

R

E

R

G

E
x
Y

A

F

P

P

A

K

V

I

L

F

G

S

W

R

A

V

D

T

R

K

R

V

Q

D

V

A

P

P

V

V

L

Q

A

G

V

M

L

L

A

T

S

I

V

V

L

I

F

I

G

Q

F

S

A

G

A

L

T

A

V

L

M

M

E

T

L

-

V

I

F

S

P

P

-

S

-

L

D

N

R

S

V

Q

L

F

P

N

V

V

L

L

L

V

N

N

I

L

V

A

G

V

S

V

T

T

C

N

L

I

V

P

W

Y

T

I

L

L

S

S

L

M

L

A

S

A

Q

L

L

V

V

I

L

I

R

Q

R

K

R

V

A

A

D

N

E77 (≠ A89) mutation E->A,D,N,Q: Strong decrease in both uptake and excretion activities.
Y78 (≠ D90) mutation to L: Uptake activity decreases more than excretion activity.
K82 (≠ P94) mutation to A: Slight decrease in both uptake and excretion activities.
Y90 (≠ W102) mutation to L: Uptake activity decreases more than excretion activity.
Y92 (≠ W104) mutation to L: Moderate decrease in both uptake and excretion activities.
W201 (≠ F218) mutation W->F,L,Y: Strong decrease in both uptake and excretion activities.
E207 (= E224) mutation E->A,D,N,Q: Lack of both uptake and excretion activities.
C210 (≠ A227) mutation to A: Moderate decrease in both uptake and excretion activities.
C285 (≠ L301) mutation to A: Moderate decrease in both uptake and excretion activities.
C286 (≠ L302) mutation to A: Moderate decrease in both uptake and excretion activities.
W292 (≠ N308) mutation W->F,L,Y: Strong decrease in both uptake and excretion activities.
K301 (≠ F317) mutation to A: Excretion activity decreases more than uptake activity.
Y308 (≠ E324) mutation to L: Excretion activity decreases more than uptake activity.

Sites not aligning to the query:

62 mutation C->A,T: Strong decrease in both uptake and excretion activities.; C→S: Moderate decrease in both uptake and excretion activities.
68 K→A: Slight decrease in both uptake and excretion activities.
422 W→L: Uptake activity decreases more than excretion activity.
425 Y→F: Moderate decrease in both uptake and excretion activities.; Y→L: Strong decrease in both uptake and excretion activities.
433 mutation E->A,D,N,Q: Strong decrease in both uptake and excretion activities.

Query Sequence

>WP_057509355.1 NCBI__GCF_001431535.1:WP_057509355.1
MPANPTPAAPASPPRLGHALKPRQLIMMGLGSAIGAGLFLGSGVGVQLAGPAVLLSYLVA
GALVIIVMNALGEMAANKPTSGAFSVYAADALGPTAGATVGWLWWVQLVIVIAAEAVGAA
GLLATVWTGLSVPMAALAFMLFFTAINLLGVKNFGEFEFWFAILKVAAILGFIAIGAALL
LGWLPDATSPGLSNFTGNGGFAPTGLAGVGAALLVVIFAFGGTEIVAVAAAETEDPERSI
ARAIRTVAWRILVFYIGSLSVIIAVVPWTSEALKSPFAAVLEAANIPGAGTAITLVAVIA
LLSALNANLYGASRMIFSLAQRREAPAVLGWADRRQVPVLAVLASVLFGFAATVMELVFP
DRVLPVLLNIVGSTCLLVWTLSLLSQLVLRRRADRAGVALPFRMAAFPWLTALALAILAL
IFALLLYGDQTRLQFLSMVVLTLFIAGCSAAARRLRSQAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory