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Comparing WP_057687228.1 NCBI__GCF_001431535.1:WP_057687228.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
59% identity, 97% coverage: 6:337/342 of query aligns to 3:332/337 of P23247
- C132 (= C134) active site, Acyl-thioester intermediate
2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
59% identity, 97% coverage: 6:337/342 of query aligns to 2:331/336 of 2r00C
Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
51% identity, 96% coverage: 6:335/342 of query aligns to 3:338/346 of Q04797
- S98 (= S101) modified: Phosphoserine
- Y146 (= Y150) modified: Phosphotyrosine
4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/359 of 4r5hA
- binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ G79), T94 (≠ S100), S95 (= S101), R98 (= R104), N126 (= N133), C127 (= C134), Q154 (= Q161), G158 (= G165), K222 (= K218), R244 (= R240), H251 (= H247)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), V13 (= V19), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), S70 (= S76), A71 (= A77), G72 (= G78), T75 (≠ V81), N93 (= N99), T94 (≠ S100), P125 (= P132), N126 (= N133), C127 (= C134), G160 (= G167), M161 (≠ R168), G328 (= G325)
4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/359 of 4r4jA
- binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: T94 (≠ S100), S95 (= S101), R98 (= R104), N126 (= N133), C127 (= C134), Q154 (= Q161), G158 (= G165), E219 (= E215), K222 (= K218), R244 (= R240), H251 (= H247)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), V13 (= V19), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), S70 (= S76), A71 (= A77), G72 (= G78), T75 (≠ V81), N93 (= N99), T94 (≠ S100), N126 (= N133), C127 (= C134), G160 (= G167), M161 (≠ R168), G328 (= G325)
3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/359 of 3pyxB
- binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (= R104), G158 (= G165), E219 (= E215), K222 (= K218), R244 (= R240)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), V13 (= V19), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), S70 (= S76), A71 (= A77), G72 (= G78), T75 (≠ V81), C127 (= C134), S157 (= S164), G158 (= G165), G160 (= G167), M161 (≠ R168), N324 (= N321), L325 (≠ I322)
4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/357 of 4r54A
- binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G78), S73 (≠ G79), T94 (≠ S100), S95 (= S101), R98 (= R104), K222 (= K218)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), V13 (= V19), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), S70 (= S76), A71 (= A77), G72 (= G78), T75 (≠ V81), N93 (= N99), T94 (≠ S100), N126 (= N133), C127 (= C134), G160 (= G167), G328 (= G325)
4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/357 of 4r41A
- binding 4-nitro-2-phosphonobenzoic acid: S70 (= S76), G72 (= G78), S73 (≠ G79), N93 (= N99), T94 (≠ S100), S95 (= S101), R98 (= R104), K222 (= K218)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), A71 (= A77), G160 (= G167), M161 (≠ R168), G162 (≠ S169)
4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/357 of 4r3nA
- active site: C127 (= C134), Q154 (= Q161), R244 (= R240), H251 (= H247)
- binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G79), T94 (≠ S100), S95 (= S101), R98 (= R104), N126 (= N133), K222 (= K218)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), V13 (= V19), A35 (= A41), S36 (= S42), S39 (= S45), T56 (≠ L62), S70 (= S76), A71 (= A77), G72 (= G78), N93 (= N99), T94 (≠ S100), N126 (= N133), C127 (= C134), G160 (= G167), M161 (≠ R168), G328 (= G325)
3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/357 of 3q1lA
3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/357 of 3pwsA
- binding (2R)-2-aminohexanedioic acid: R98 (= R104), N126 (= N133), G158 (= G165), I208 (= I204), E219 (= E215), K222 (= K218), R244 (= R240)
- binding adenosine-2'-5'-diphosphate: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), A71 (= A77), T75 (≠ V81), G160 (= G167), M161 (≠ R168)
3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/357 of 3pwkA
- binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), A71 (= A77), T75 (≠ V81), G160 (= G167)
- binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (= R104), N126 (= N133), G158 (= G165), A159 (≠ G166), E219 (= E215), K222 (= K218), R244 (= R240)
2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/357 of 2gz3A
- active site: C127 (= C134), Q154 (= Q161), R244 (= R240), H251 (= H247)
- binding (2r)-2-amino-4-oxobutanoic acid: C127 (= C134), Q154 (= Q161), G158 (= G165), E219 (= E215), R244 (= R240), H251 (= H247)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), V13 (= V19), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), S70 (= S76), A71 (= A77), G72 (= G78), T75 (≠ V81), N93 (= N99), G158 (= G165), G160 (= G167), M161 (≠ R168), N324 (= N321), A329 (= A326)
2gz2A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with 2',5'-adp (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/357 of 2gz2A
- active site: C127 (= C134), Q154 (= Q161), R244 (= R240), H251 (= H247)
- binding adenosine-2'-5'-diphosphate: G8 (= G14), T10 (= T16), G11 (= G17), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), A71 (= A77), T75 (≠ V81)
2gz1A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/357 of 2gz1A
- active site: C127 (= C134), Q154 (= Q161), R244 (= R240), H251 (= H247)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), V13 (= V19), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), S70 (= S76), A71 (= A77), G72 (= G78), T75 (≠ V81), N93 (= N99), S157 (= S164), G158 (= G165), G160 (= G167), M161 (≠ R168), N324 (= N321), L325 (≠ I322)
3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/358 of 3q11A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), T10 (= T16), G11 (= G17), A35 (= A41), S36 (= S42), R38 (= R44), S39 (= S45), T56 (≠ L62), A71 (= A77), T75 (≠ V81), G160 (= G167), M161 (≠ R168), G162 (≠ S169)
- binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R104), N126 (= N133), C127 (= C134), Q154 (= Q161), G158 (= G165), E219 (= E215), K222 (= K218), R244 (= R240)
4r51A Crystal complex structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide adenine dinucleotide phosphate and phthalic acid (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/360 of 4r51A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), T10 (= T16), G11 (= G17), A12 (= A18), V13 (= V19), A35 (= A41), S36 (= S42), S39 (= S45), T56 (≠ L62), S70 (= S76), A71 (= A77), G72 (= G78), N93 (= N99), T94 (≠ S100), N126 (= N133), C127 (= C134), G160 (= G167), M161 (≠ R168), G328 (= G325)
- binding phthalic acid: S73 (≠ G79), T94 (≠ S100), S95 (= S101), R98 (= R104), N126 (= N133), K222 (= K218)
3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
45% identity, 96% coverage: 8:335/342 of query aligns to 2:338/361 of 3pylC
3tz6A Crystal structure of aspartate semialdehyde dehydrogenase complexed with inhibitor smcs (cys) and phosphate from mycobacterium tuberculosis h37rv (see paper)
46% identity, 97% coverage: 8:339/342 of query aligns to 2:342/342 of 3tz6A
- active site: C129 (= C134), Q156 (= Q161), R248 (= R240), H255 (= H247)
- binding cysteine: C129 (= C134), Q156 (= Q161), G160 (= G165), E223 (= E215), R248 (= R240), H255 (= H247)
- binding glycerol: S108 (= S114), G187 (= G179), F192 (= F184), P201 (≠ Q195), Q225 (≠ M217), R228 (≠ V220), F229 (≠ W221), Q335 (= Q332), E338 (= E335), L339 (= L336)
- binding sulfate ion: R98 (= R104), H117 (≠ N123), R119 (= R124), N128 (= N133), C129 (= C134), K226 (= K218), E270 (≠ A262), R273 (= R265)
Q57658 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
29% identity, 89% coverage: 4:306/342 of query aligns to 5:320/354 of Q57658
Sites not aligning to the query:
Query Sequence
>WP_057687228.1 NCBI__GCF_001431535.1:WP_057687228.1
MSTEQRSFHVAVVGATGAVGETMLSILAERDFPVGTLTLLASSRSAGGEVEFNGHKHIIK
DLADFDPTGVDIALFSAGGSVSKDYAPKFAAAGAVVIDNSSAFRYDDDVPLVVSEVNPDQ
VANRPRGIIANPNCSTMQMLVALAPLHRRYGIERINVATYQSVSGGGRSALEELGKQTGQ
LLSFQEIDPQRFPVQIAFNLIPHIDDFLDNGFTKEEMKLVWETQKILGDSSILVNPTAVR
VPVFYGHSEAVAIETRDKVTAAQARELLSQSPGVEVVDRHEAGGYPTPVTHASGTDAVYV
GRIREDLSHPRGLNLWIVSDNIRKGAALNAVQLAELVAAEAR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory