SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_057687364.1 NCBI__GCF_001431535.1:WP_057687364.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q6LX42 Sodium/alanine symporter AgcS; Alanine permease; Alanine/glycine:cation symporter from Methanococcus maripaludis (strain S2 / LL) (see paper)
36% identity, 94% coverage: 5:474/501 of query aligns to 7:447/453 of Q6LX42

query
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Q6LX42

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T75 (≠ R70) binding ; binding
G79 (= G74) binding
N80 (= N75) binding ; mutation to A: Markedly reduces L-alanine uptake; when associated with A-274 and A-308.
I165 (≠ L156) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
Q170 (= Q161) binding ; binding
F273 (≠ Y283) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
FS 273:274 (≠ YA 283:284) binding
FSNE 273:276 (≠ YANE 283:286) binding
S274 (≠ A284) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-308.
D308 (= D318) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-274.
W370 (≠ Y393) mutation to Q: Does not change uptake of L-alanine, but reduces the ability to transport D-alanine.

6csfM Crystal structure of sodium/alanine symporter agcs with d-alanine bound (see paper)
36% identity, 92% coverage: 13:474/501 of query aligns to 1:427/431 of 6csfM

query
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6csfM

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binding d-alanine: T58 (≠ R70), G60 (= G72), G62 (= G74), N63 (= N75), Q150 (= Q161), F253 (≠ Y283), S254 (≠ A284)
binding sodium ion: T58 (≠ R70), I59 (≠ M71), N63 (= N75), S254 (≠ A284), D288 (= D318)

6cseM Crystal structure of sodium/alanine symporter agcs with l-alanine bound (see paper)
36% identity, 92% coverage: 13:474/501 of query aligns to 1:427/431 of 6cseM

query
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6cseM

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K

V

T

S

D

H

H

P

P

A

G

K

R

Q

Q

G

A

Y

L

V

V

Q

S

A

M

F

T

A

G

I

T

Y

F

F

L

D
|
D

T

T

M

I

M

V

V

V

C

C

T

T

A

I

T

T

A

G

F

L

L

V

I

L

L

T

A

I

S

A

G

G

T

L

Y

-

N

-

V

-

Y

-

S

-

P

-

V

-

A

-

G

-

A

-

E

-

P

K

I

A

F

F

Q

P

G

G

L

L

K

T

G

D

I

L

A

-

E

T

G

G

A

A

G

S

Y

L

A

T

Q

A

A

A

G

S

V

F

E

D

A

A

V

L

L

M

P

P

G

-

W

M

G

G

A

G

S

L

F

I

V

V

A

T

I

I

A

G

I

L

F

V

F

F

F

F

A

A

F

Y

T

S

T

T

I

V

M

L

A

G

Y

W

Y

S

Y

Y

M

Y

A

G

E

E

T

K

N

C

L

F

T

E

Y

Y

L

L

N

I

N

G

N

T

A

K

K

G

R

-

P

-

L

-

T

-

V

I

L

R

L

L

L

Y

R

R

L

I

G

A

I

F

I

V

G

L

M

V

V

A

I

F

F

W

G

G

A

A

F

T

H

A

N

S

A

L

Q

P

L

L

A

V

W

W

A

N

L

I

G

A

D

D

I

T

G

L

V

N

G

G

L

A

M

M

A

A

W

I

L

P

N

N

I

L

V

I

A

G

I

L

L

L

I

L

V

L

Q

S

K

G

P

V

A

V

M

V

I

S

A

E

L

T

R

K

D

A

Y

F

E

N

R

E

Q

I

K

R

K

K

binding alanine: A57 (≠ G69), T58 (≠ R70), G60 (= G72), N63 (= N75), Q150 (= Q161), F253 (≠ Y283), S254 (≠ A284), E256 (= E286)
binding sodium ion: T58 (≠ R70), I59 (≠ M71), N63 (= N75), S254 (≠ A284), D288 (= D318)

Query Sequence

>WP_057687364.1 NCBI__GCF_001431535.1:WP_057687364.1
MEATVHFINSIIWSKALIFMCLAAGLYFSVRTRFMQIRGFFEMCRLTVAGEKSDAGVSSF
QALAMSMAGRMGIGNIAGVATAIAFGGPGAIFWMWVMGFLGASTSYVECTLAQIYKTKDA
EGRYRGGPAYYIEKAMGLKWYAMAFALATIVAAGFLMPGVQANAIADSIINACRGGALCG
PLDGQAFGMDQVQALKLGIGVVVAVLLAVVIFGGVKRIANFAEIVVPFMAAGFIIMAIVI
MIMNADQVPVMFNTIFSSAFGTHAAFGAMIGLAVEWGVKRGIYANEAGQGTGPHAAAASE
VSHPAKQGYVQAFAIYFDTMMVCTATAFLILASGTYNVYSPVAGAEPIFQGLKGIAEGAG
YAQAGVEAVLPGWGASFVAIAIFFFAFTTIMAYYYMAETNLTYLNNNAKRPLTVLLLRLG
IIGMVIFGAFHNAQLAWALGDIGVGLMAWLNIVAILIVQKPAMIALRDYERQKKLGLDPT
FDPVALGIKNADFWQKKQEAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory