SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_058857680.1 NCBI__GCF_001482365.1:WP_058857680.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
26% identity, 66% coverage: 29:324/447 of query aligns to 36:318/444 of Q8NLB7

query
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Q8NLB7

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D54 (≠ E44) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (= D47) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (≠ K99) mutation to A: Loss of transport activity.
W309 (≠ L315) mutation to V: Loss of transport activity.
D312 (= D318) mutation to A: Loss of transport activity.
R313 (= R319) mutation to A: Loss of transport activity.
I317 (≠ K323) mutation I->H,Y: Loss of transport activity.

Sites not aligning to the query:

386 R→A: Loss of transport activity.

P36035 Carboxylic acid transporter protein homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 70% coverage: 84:395/447 of query aligns to 186:501/616 of P36035

query
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P36035

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K338 (vs. gap) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
9 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
24% identity, 82% coverage: 24:390/447 of query aligns to 14:384/452 of Q5EXK5

query
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Q5EXK5

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D82 (= D98) mutation to A: Loss of activity.
V311 (≠ L315) mutation to W: Loss of activity.
D314 (= D318) mutation to A: Loss of activity.

8fvzA Pipt y150a
22% identity, 93% coverage: 32:447/447 of query aligns to 4:429/433 of 8fvzA

query
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8fvzA

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T

V

G

I

V

Q

I

A

A

P

S

V

K

R

K

D

A

M

H

F

W

R

Q

-

E

-

F

-

V

-

A

-

Y

-

F

-

S

-

T

-

W

N

N

H

H

W

F

G

R

A

N

L

L

R

G

A

S

V

M

G

L

A

G

V
x
W

L

F

L

L

N

V

A
x
D

V

I

G

A

F

F

Y
|
Y

V

G

L

I

L

N

S

L

Y

N

M

Q

P

S

T

V

Y

V

L

L

S

A

E

Q

E

I

I

G

G

F

L

A

G

G

K

K

T

T

E

G

S

D

F

V

-

Y

-

D

-

K

-

L

-

F

-

Q

L

L

A

A

T

T

S

G

L

N

A

I

L

I

L

V

S

T

Y

A

V

L

G

G

F

F

I

L

-

P

-

G

-

Y

F

Y

L

F

T

T

G

L

L

F

A

L

S

I

D

D

R

I

F

V

G

G

R

R

K

K

R

K

M

L

L

-

M

-

A

-

S

-

V

-

S

-

F

-

V

-

L

-

T

-

V

-

P

-

A

Q

F

F

M

M

-

G

V

F

L

I

E

M

S

S

G

G

T

L

F

F

L

L

A

A

V

I

L

L

A

A

V

G

Q

E

V

I

-

D

-

H

-

I

-

G

-

K

-

G

-

P

L

L

G

A

A

C

M

F

L

T

T

F

L

M

N

Q

-

F

-

N

-

F

D

G

G

A

T
x
N

L

T

P

T

S

T

F

F

L

I

-

V

-

A

A

A

E

E

L

L

F

F

P

P

T

T

R

R

I

I

R

R

Y

A

T

S

G

A

F

H

A

G

V

I

S

S

F

-

N

-

L

-

A

-

N

-

A

A

V

A

F

A

G

G

G

K

T

C

A

G

P

A

F

I

M

L

A

S

T

S

L

L

L

V

I

F

S

N

A

Q

F

L

A

K

T

A

N

K

L

I

A

G

P

T

A

S

-

A

-

V

-

L

W

W

Y

I

L

F

M

F

A

S

A

T

A

C

A

I

V

L

S

G

F

F

V

I

A

S

V

T

A

F

L

L

S

I

R

D

E

E

T

T

G

M

G

G

-

V

-

D

-

P

-

D

-

E

K

K

P

D

L

L

E

E

H

E

binding phosphate ion: F145 (≠ P176), W235 (≠ V266), D239 (≠ A270), Y243 (= Y274), N346 (≠ T369)

8bvtA Cryo-em structure of rat slc22a6 bound to probenecid (see paper)
35% identity, 18% coverage: 88:168/447 of query aligns to 138:210/508 of 8bvtA

query
sites
8bvtA

L

L

G

G

G

A

F

M

V

V

W

F

G

G

H

Y

L

L

G

A

D

D

K

R

L

L

G

G

R

R

T

K

A

V

L

L

S

I

L

L

S

N

I

Y

L

L

I

Q

M

T

S

A

V

V

S

S

T

G

F

T

C

C

I

A

A

A

L

Y

I

A

P

P

G

N

Y

Y

A

T

T

V

I

Y

G

C

I

V

F

F

A

-

P

-

V

-

L

-

V

-

R

R

I

L

V

L

Q

S

G

G

F

M

S

S

A

L

A

A

G

S

E

I

Y

A

A

I

G

N

A

C

S
x
M

A

T

F

L

L

N

V

V

E

E

Y

W

A

M

P

P

A

I

N

H

R

T

R

R

binding 4-(dipropylsulfamoyl)benzoic acid: M198 (≠ S156)

Sites not aligning to the query:

binding 4-(dipropylsulfamoyl)benzoic acid: 31, 35, 217, 221, 339

8bw7A Cryo-em structure of rat slc22a6 bound to alpha-ketoglutaric acid (see paper)
35% identity, 18% coverage: 88:168/447 of query aligns to 129:201/497 of 8bw7A

query
sites
8bw7A

L

L

G

G

G

A

F

M

V

V

W

F

G

G

H

Y

L

L

G

A

D

D

K

R

L

L

G

G

R

R

T

K

A

V

L

L

S

I

L

L

S

N

I

Y

L

L

I

Q

M

T

S

A

V

V

S

S

T

G

F

T

C

C

I

A

A

A

L

Y

I

A

P

P

G

N

Y

Y

A

T

T

V

I

Y

G

C

I

V

F

F

A

-

P

-

V

-

L

-

V

-

R

R

I

L

V

L

Q

S

G

G

F

M

S

S

A

L

A

A

G

S

E

I

Y

A

A

I

G

N

A

C

S

M

A

T

F

L

L

N

V

V

E

E

Y

W

A

M

P

P

A

I

N

H

R

T

R

R

Sites not aligning to the query:

binding 2-oxoglutaric acid: 209, 212, 354

8bvsA Cryo-em structure of rat slc22a6 bound to tenofovir (see paper)
35% identity, 18% coverage: 88:168/447 of query aligns to 129:201/502 of 8bvsA

query
sites
8bvsA

L

L

G

G

G

A

F

M

V

V

W

F

G

G

H

Y

L

L

G

A

D

D

K

R

L

L

G

G

R

R

T

K

A

V

L

L

S

I

L

L

S

N

I

Y

L

L

I

Q

M

T

S

A

V

V

S

S

T

G

F

T

C

C

I

A

A

A

L

Y

I

A

P

P

G

N

Y

Y

A

T

T

V

I

Y

G

C

I

V

F

F

A

-

P

-

V

-

L

-

V

-

R

R

I

L

V

L

Q

S

G

G

F

M

S

S

A

L

A

A

G

S

E

I

Y

A

A

I

G

N

A

C

S

M

A

T

F

L

L

N

V

V

E

E

Y

W

A

M

P

P

A

I

N

H

R

T

R

R

Sites not aligning to the query:

binding [2-(6-amino-9h-purin-9-yl)-1-methylethoxy]methylphosphonic acid: 212, 330

A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
22% identity, 79% coverage: 94:447/447 of query aligns to 63:432/446 of A0A0H2VG78

query
sites
A0A0H2VG78

G

G

H

P

L

L

G

A

D

D

K

K

L

L

G

G

R

R

T

R

A

L

L

V

S

M

L

L

S

I

I

A

L

I

I

V

M

F

S

I

V

I

S

G

T

A

F

L

C

I

I

L

A

A

L

A

I

S

P

T

G

N

Y

L

A

A

T

-

I

-

G

-

I

-

F

-

A

-

P

-

V

-

L

L

L

I

V

I

V

G

R
|
R

I

L

V

I

Q
x
I

G

G

F

L

S

A

A

V

A

G

G

G

E

S

Y

M

A

S

G

T

A

V

S

P

A

V

F

Y

L

L

V

S

E
|
E

Y

M

A

A

P

P

A

T

N

E

R

Y

R

R

G

G

L

S

Y

L

A

G

A

S

V

L

V

N

P
x
Q

A

L

S

M

T

I

A

T

T

I

G

G

L

I

L

L

F

A

G

A

S

Y

L

L

F

-

V

-

A

-

G

-

L

V

T

N

A

Y

L

A

L

F

S

A

D

D

D

I

Q

E

L

-

N

-

S

-

W

-

G

G

W

W

R

R

-

W

-

M

-

L

-

G

-

L

-

A

-

V

-

P

-

S

-

V

-

I

-

L

-

V

-

G

I

I

P

Y

F

F

L

M

L

P

A

E

A

S

P

P

M

R

G

W

L

L

I

L

G

E

-

N

-

R

-

N

-

E

-

A

-

R

R

Q

Y

V

I

M

R

K

T

I

K

T

L

Y

E

D

D

D

T

S

P

E

A

I

F

D

Q

K

E

E

L

L

A

K

E

E

E

M

D

K

E

E

V

I

-

N

-

A

I

I

Q

S

A

E

P

S

V

T

R

W

D

T

M

V

F

I

R

K

N

S

H

P

W

W

G

L

A

G

L

R

L

I

R

L

A

I

V

V

G

G

A

C

V

I

L

F

-

A

-

I

-

F

-
x
Q

-

F

-

I

-

G

L

I

N
|
N

A

A

V

V

G

I

F

F

Y

Y

V

-

L

-

S

-

Y

-

M

-

P

S

T

S

Y

S

L

I

S

F

E

A

E

K

I

A

G

G

L

L

G

G

K

E

T

A

E

A

S

S

F

I

L

L

A

G

T

S

-

V

-

G

-

I

-

G

S

T

L

I

A

N

L

V

L

L

S

V

Y

T

V

I

G

V

F

A

I

I

F

F

L

V

T

V

G

-

L

-

A

-

S

-

D

D

R

K

F

I

G

D

R

R

K

K

R

K

M

L

L

L

M

V

A

G

S

N

V

I

S

G

F

M

V

I

V

A

-

S

L

L

T

L

V

I

P

M

A

A

F

I

M

L

V

I

L

W

E

T

S

I

G

G

T

I

F

A

L

S

A

S

V

A

L

W

A

I

V

I

Q

I

V

V

L

C

L

L

G

S

A

L

M

F

L

I

T

V

-

F

-

G

L

I

N

S

D

W

G

G

-

P

T

V

L

L

P
x
W

S

V

F

M

L

L

A

P

E

E

L

L

F

F

P

P

T

M

R

R

I

A

R

R

Y

G

T

A

G

A

F

T

A

G

V

I

S

S

F

A

N

L

L

V

A

L

N

N

A

-

V

-

F

-

G

-

T

-

A

-

P

-

F

-

M

I

A

G

T

T

L

L

L

I

I

V

S

S

A

L

F

F

-

F

-

P

-

I

-

L

A

S

T

D

N

A

L

L

A

S

P

T

A

E

W

W

Y

V

L

F

M

L

A

I

A

F

A

A

A

F

V

I

S

G

F

V

V

L

A

A

-

M

-

I

-

F

-

V

V

I

A

K

L

F

S

L

R

P

E

E

T

T

G

R

G

G

K

R

P

S

L

L

E

E

H

E

R102 (= R141) mutation to A: Loss of transport activity.
I105 (≠ Q144) mutation to S: Affects symport activity. May function as an uniporter.
E122 (= E161) mutation to A: Loss of transport activity.
Q137 (≠ P176) mutation to A: Loss of transport activity.
Q250 (vs. gap) mutation to A: Loss of transport activity.
Q251 (vs. gap) mutation to A: Loss of transport activity.
N256 (= N269) mutation to A: Loss of transport activity.
W357 (≠ P371) mutation to A: Loss of transport activity.

Sites not aligning to the query:

22 D→N: Affects symport activity. May function as an uniporter.

8uo9A Structure of synaptic vesicle protein 2a in complex with a nanobody (see paper)
30% identity, 18% coverage: 88:169/447 of query aligns to 65:138/555 of 8uo9A

query
sites
8uo9A

L

V

G

G

G

A

F

F

V

L

W

W

G

G

H

G

L

L

G

A

D

D

K

R

L

L

G

G

R

R

T

Q

A

C

L

L

S

L

L

I

S

S

I

L

L

S

I

V

M

N

S

S

V

V

S

F

T

A

F

F

C

F

I

S

A

S

L

F

I

V

P

Q

G

G

Y

Y

A

G

T

T

I

-

G

-

I

-

F

-

A

-

P

-

V

-

L

-

L

F

L

L

V

F

V

C

R

R

I

L

V

L

Q

S

G

G

F

V

S

G

A

I

A

G

G

G

E

S

Y

I

A

P

G

I

A

V

S

F

A

S

F

Y

L

F

V

S

E

E

Y

F

A

L

P

A

A

Q

N

E

R

K

R

R

G

G

Sites not aligning to the query:

P25297 Inorganic phosphate transporter PHO84 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
26% identity, 33% coverage: 88:235/447 of query aligns to 117:277/587 of P25297

query
sites
P25297

L

I

G

G

G

Q

F

F

V

G

W

F

G

G

H

T

L

L

G

A

D

D

K

I

L

V

G

G

R

R

R

K

T

R

A

I

L

Y

S

G

L

M

S

E

I

L

L

I

I

I

M

M

S

I

V

V

S
x
C

T

T

F

I

C

L

I

Q

A

T

L

T

I

V

P

A

G

H

Y

S

A

P

T

A

I

I

G

N

I
x
F

F

V

A

A

P

-

V

-

L

V

L

L

L

T

V

F

V

Y

R
|
R

I

I

V

V

Q

M

G
|
G

F

I

S
x
G

A

I

A
x
G

G

G

E
x
D

Y
|
Y

A

P

G

L

A

S

S

S

A

I

F

I

L

T

V

S

E

E

Y

F

A

A

P

T

A

T

N

K

R

W

R

R

G

G

L

A

Y

I

A

M

A

G

V

A

V

V

P

F

A

A

S

N

T

Q

A

A

T

W

G

G

L

Q

L

I

F

S

G

G

S

G

L

I

F

I

V

A

A

L

G

I

L

L

T

V

A

A

L

A

L

Y

S

K

D

G

-

E

-

L

-

E

-

Y

-

A

-

N

-

S

-

G

-

A

-

E

-
x
C

-

D

-

A

-

R

-
x
C

-

Q

-

K

-

A

-
x
C

D

D

Q

Q

L

M

N

-

S

-

W

-

G

-

W

W

R

R

I

I

P

L

F

I

L

G

L

L

A

G

A

T

P

V

M

L

G

G

L

L

I

A

G
x
C

R

L

Y

Y

I

F

R

R

T

L

K

T

L

I

E

P

D

E

T

S

P

P

A

R

F

Y

Q

Q

C145 (≠ S116) mutation to S: No significant effect on transport activity; when associated with S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
F160 (≠ I131) mutation to C: Reduces phosphate binding, transport and signaling activities.
R168 (= R141) mutation to A: Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates.; mutation to C: Abolishes transport and signaling activities.; mutation to E: Reduces transport activity. Moderately reduces affinity for inorganic phosphate. No significant effects on growth rates.; mutation to Q: Reduces transport activity. Moderately reduces affinity for inorganic phosphate. Moderately reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
G172 (= G145) mutation to C: Abolishes transport and signaling activities.
G174 (≠ S147) mutation to C: Abolishes transport and signaling activities.
G176 (≠ A149) mutation to C: Abolishes transport and signaling activities.
D178 (≠ E151) mutation D->E,N: Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on signaling activities. No significant effects on growth rates.
Y179 (= Y152) mutation to A: Significantly reduces transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Slightly reduces signaling activity.; mutation to G: Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Abolishes signaling activity.; mutation Y->S,F: No significant effects on transport activity. No significant effects on growth rates under low phosphate conditions. Does not affect patterns of secreted phosphatase activities under low and high phosphate conditions. No significant effects on signaling activity.
C237 (vs. gap) mutation to S: No significant effect on transport activity; when associated with S-145, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
C241 (vs. gap) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
C245 (vs. gap) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
C263 (≠ G221) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.

Sites not aligning to the query:

6 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
298 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); K→A: No significant effect on membrane localization under low phosphate conditions and internalization after phosphate addition. Strongly affects vacuolar sorting of the protein after internalization.
304:327 mutation Missing: Increases transport activity. Has no significant effect on membrane localization under low phosphate conditions. Results in severely delayed internalization after phosphate addition.
335 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-399, S-434, S-455, S-474, S-510 and S-519.
358 D→E: Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Moderately decreases signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.; D→N: Significantly reduces transport activity. No significant effects on affinity for inorganic phosphate. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. No significant effects on signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
392 V→C: Reduces transport and signaling activities.
399 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-434, S-455, S-474, S-510 and S-519.
408 V→C: Reduces signaling activity and enhances transport activity.
434 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-455, S-474, S-510 and S-519.
455 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-474, S-510 and S-519.
473 E→K: No significant effects on transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates.; E→Q: No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates.
474 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-510 and S-519.
492 mutation K->A,Q: No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates.; K→E: Reduces transport activity. Reduces affinity for inorganic phosphate. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
510 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474 and S-519.
519 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474 and S-510.

8k77A Synaptic vesicle glycoprotein 2A (see paper)
30% identity, 18% coverage: 88:169/447 of query aligns to 81:154/592 of 8k77A

query
sites
8k77A

L

V

G

G

G

A

F

F

V

L

W

W

G

G

H

G

L

L

G

A

D

D

K

R

L

L

G

G

R

R

T

Q

A

C

L

L

S

L

L

I

S

S

I

L

L

S

I

V

M

N

S

S

V

V

S

F

T

A

F

F

C

F

I

S

A

S

L

F

I

V

P

Q

G

G

Y

Y

A

G

T

T

I

-

G

-

I

-

F

-

A

-

P

-

V

-

L

-

L

F

L

L

V

F

V

C

R

R

I

L

V

L

Q

S

G

G

F

V

S

G

A

I

A

G

G

G

E

S

Y

I

A

P

G

I

A

V

S

F

A

S

F

Y

L

F

V

S

E

E

Y

F

A

L

P

A

A

Q

N

E

R

K

R

R

G

G

Sites not aligning to the query:

binding (2S)-2-[(4R)-2-oxidanylidene-4-propyl-pyrrolidin-1-yl]butanamide: 164, 311, 516, 520

8jlhA Cryo-em structure of sv2a dimer in complex with bont/a2 hc and levetiracetam (see paper)
30% identity, 18% coverage: 88:169/447 of query aligns to 81:154/592 of 8jlhA

query
sites
8jlhA

L

V

G

G

G

A

F

F

V

L

W

W

G

G

H

G

L

L

G

A

D

D

K

R

L

L

G

G

R

R

T

Q

A

C

L

L

S

L

L

I

S

S

I

L

L

S

I

V

M

N

S

S

V

V

S

F

T

A

F

F

C

F

I

S

A

S

L

F

I

V

P

Q

G

G

Y

Y

A

G

T

T

I

-

G

-

I

-

F

-

A

-

P

-

V

-

L

-

L

F

L

L

V

F

V

C

R

R

I

L

V

L

Q

S

G

G

F

V

S

G

A

I

A

G

G

G

E

S

Y

I

A

P

G

I

A

V

S

F

A

S

F

Y

L

F

V

S

E

E

Y

F

A

L

P

A

A

Q

N

E

R

K

R

R

G

G

Sites not aligning to the query:

8jlcA Synaptic vesicle glycoprotein 2A (see paper)
30% identity, 18% coverage: 88:169/447 of query aligns to 81:154/592 of 8jlcA

query
sites
8jlcA

L

V

G

G

G

A

F

F

V

L

W

W

G

G

H

G

L

L

G

A

D

D

K

R

L

L

G

G

R

R

T

Q

A

C

L

L

S

L

L

I

S

S

I

L

L

S

I

V

M

N

S

S

V

V

S

F

T

A

F

F

C

F

I

S

A

S

L

F

I

V

P

Q

G

G

Y

Y

A

G

T

T

I

-

G

-

I

-

F

-

A

-

P

-

V

-

L

-

L

F

L

L

V

F

V

C

R

R

I

L

V

L

Q

S

G

G

F

V

S

G

A

I

A

G

G

G

E

S

Y
x
I

A

P

G

I

A

V

S

F

A

S

F

Y

L

F

V

S

E

E

Y

F

A

L

P

A

A

Q

N

E

R

K

R

R

G

G

binding (2S)-2-(2-oxidanylidenepyrrolidin-1-yl)butanamide: I137 (≠ Y152)

Sites not aligning to the query:

binding (2S)-2-(2-oxidanylidenepyrrolidin-1-yl)butanamide: 164, 516, 520

Q02563 Synaptic vesicle glycoprotein 2A; Synaptic vesicle protein 2; Synaptic vesicle protein 2A from Rattus norvegicus (Rat) (see 2 papers)
30% identity, 19% coverage: 88:171/447 of query aligns to 217:292/742 of Q02563

query
sites
Q02563

L

V

G

G

G

A

F

F

V

L

W

W

G

G

H

G

L

L

G

A

D

D

K

R

L

L

G

G

R

R

T

Q

A

C

L

L

S

L

L

I

S

S

I

L

L

S

I

V

M

N

S

S

V

V

S

F

T

A

F

F

C

F

I

S

A

S

L

F

I

V

P

Q

G

G

Y

Y

A

G

T

T

I

-

G

-

I

-

F

-

A

-

P

-

V

-

L

-

L

F

L

L

V

F

V

C

R

R

I

L

V

L

Q

S

G

G

F

V

S

G

A

I

A

G

G

G

E

S

Y

I

A

P

G

I

A

V

S

F

A

S

F

Y

L

F

V

S

E

E

Y

F

A

L

P

A

A

Q

N

E

R

K

R

R

G

G

L

E

Y

H

Sites not aligning to the query:

196:200 mutation Missing: No change in uptake of C.botulinum neurotoxin type D (BoNT/D, botD) or C.botulinum neurotoxin type E (BoNT/E).
321:331 mutation Missing: No change in uptake of BoNT/D or BoNT/E.
498 N→Q: No change in uptake of BoNT/E or C.botulinum neurotoxin type A (BoNT/A, botA) by mouse SV2A/SV2B knockout neurons; SV2A apparent molecular weight decreases. No change in uptake of BoNT/E; when associated with Q-548. No change in uptake of BoNT/D.
548 N→Q: No change in uptake of BoNT/E or BoNT/A by mouse SV2A/SV2B knockout neurons; SV2A apparent molecular weight decreases. No change in uptake of BoNT/E; when associated with Q-498. No change in uptake of BoNT/D.
570:573 RLVN→TLVQ: Restores apparent molecular weight to wild-type, does not restore uptake of BoNT/E.
573 N→Q: BoNT/E not taken up by mouse SV2A/SV2B knockout neurons, decreased uptake of BoNT/A; SV2A apparent molecular weight decreases. No change in uptake of BoNT/D.

Q8VC69 Solute carrier family 22 member 6; Kidney-specific transport protein; Novel kidney transcript; mNKT; Organic anion transporter 1; mOAT1; Renal organic anion transporter 1; mROAT1 from Mus musculus (Mouse) (see 2 papers)
33% identity, 18% coverage: 88:168/447 of query aligns to 141:213/545 of Q8VC69

query
sites
Q8VC69

L

L

G

G

G

A

F

M

V

M

W

F

G

G

H

Y

L

L

G

A

D

D

K

R

L

L

G

G

R

R

T

K

A

V

L

L

S

I

L

L

S

N

I

Y

L

L

I

Q

M

T

S

A

V

V

S

S

T

G

F

T

C

C

I

A

A

A

L

Y

I

A

P

P

G

N

Y

Y

A

T

T

V

I

Y

G
x
C

I

I

F

F

A

-

P

-

V

-

L

-

V

-

R

R

I

L

V

L

Q

S

G

G

F

M

S

S

A

L

A

A

G

S

E

I

Y

A

A

I

G

N

A

C

S

M

A

T

F

L

L

N

V

M

E

E

Y

W

A

M

P

P

A

I

N

H

R

T

R

R

C183 (≠ G130) mutation to A: Decreased cell surface expression level and PAH transport activity. Complete loss of PAH transport activity; when associated with A-49; A-78; A-99; A-122; A-172; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.

Sites not aligning to the query:

39 N→Q: Complete loss of PAH transport activity.
49 C→A: Decreased cell surface expression level and PAH transport activity. Complete loss of PAH transport activity; when associated with A-78; A-99; A-122; A-172; A-183; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.
56 modified: carbohydrate, N-linked (GlcNAc...) asparagine
86 modified: carbohydrate, N-linked (GlcNAc...) asparagine
91 modified: carbohydrate, N-linked (GlcNAc...) asparagine
107 modified: carbohydrate, N-linked (GlcNAc...) asparagine
122 C→A: Decreased cell surface expression level and PAH transport activity. Complete loss of PAH transport activity; when associated with A-49; A-78; A-99; A-172; A-183; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.
434 C→A: Decreased cell surface expression level and PAH transport activity. 80% decrease of PAH transport activity; when associated with A-49; A-122 and A-183. Complete loss of PAH transport activity; when associated with A-49; A-78; A-99; A-122; A-172; A-183; A-200; A-362; A-335; A-379; A-402 and A-427.

Query Sequence

>WP_058857680.1 NCBI__GCF_001482365.1:WP_058857680.1
MSHSSDAAAAAAQAVPASPPAPGAPDPSVRRRVVTASFIGNFVEWFDYAIYGYLAATIAV
VFFPESDPQTALLATFALFAISFFVRPLGGFVWGHLGDKLGRRTALSLSILIMSVSTFCI
ALIPGYATIGIFAPVLLLVVRIVQGFSAAGEYAGASAFLVEYAPANRRGLYAAVVPASTA
TGLLFGSLFVAGLTALLSDDQLNSWGWRIPFLLAAPMGLIGRYIRTKLEDTPAFQELAEE
DEVIQAPVRDMFRNHWGALLRAVGAVLLNAVGFYVLLSYMPTYLSEEIGLGKTESFLATS
LALLSYVGFIFLTGLASDRFGRKRMLMAASVSFVVLTVPAFMVLESGTFLAVLAVQVLLG
AMLTLNDGTLPSFLAELFPTRIRYTGFAVSFNLANAVFGGTAPFMATLLISAFATNLAPA
WYLMAAAAVSFVAVALSRETGGKPLEH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory