SitesBLAST
Comparing WP_058857809.1 NCBI__GCF_001482365.1:WP_058857809.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
57% identity, 98% coverage: 6:259/259 of query aligns to 3:256/256 of Q48436
- 6:33 (vs. 9:36, 75% identical) binding NAD(+)
- D59 (= D62) binding NAD(+)
- K156 (= K159) binding NAD(+)
1gegE Cryatal structure analysis of meso-2,3-butanediol dehydrogenase (see paper)
57% identity, 98% coverage: 6:259/259 of query aligns to 3:256/256 of 1gegE
- active site: G13 (= G16), S139 (= S142), Y152 (= Y155), K156 (= K159), V197 (≠ F200)
- binding alpha-D-glucopyranose: R63 (= R66), D64 (= D67), F67 (≠ R70), E123 (≠ A126)
- binding nicotinamide-adenine-dinucleotide: G9 (= G12), Q12 (≠ R15), I14 (= I17), D33 (= D36), Y34 (≠ L37), V58 (≠ A61), D59 (= D62), V60 (= V63), N86 (= N89), A87 (= A90), I109 (≠ V112), S139 (= S142), Y152 (= Y155), K156 (= K159), P182 (= P185), V185 (= V188), T187 (= T190), M189 (= M192)
3wyeA Crystal structure of chimeric engineered (2s,3s)-butanediol dehydrogenase complexed with NAD+
57% identity, 98% coverage: 6:259/259 of query aligns to 2:255/255 of 3wyeA
- active site: G12 (= G16), S138 (= S142), Y151 (= Y155), K155 (= K159), L196 (≠ F200)
- binding nicotinamide-adenine-dinucleotide: G8 (= G12), Q11 (≠ R15), G12 (= G16), I13 (= I17), D32 (= D36), Y33 (≠ L37), V57 (≠ A61), D58 (= D62), V59 (= V63), N85 (= N89), A86 (= A90), S138 (= S142), Y151 (= Y155), K155 (= K159), P181 (= P185), G182 (= G186), V184 (= V188), T186 (= T190), M188 (= M192), W189 (= W193)
Q9ZNN8 L-2,3-butanediol dehydrogenase; L-BDH; (S,S)-butanediol dehydrogenase; Diacetyl reductase [(S)-acetoin forming]; EC 1.1.1.76; EC 1.1.1.304 from Corynebacterium glutamicum (Brevibacterium saccharolyticum) (see paper)
52% identity, 98% coverage: 5:259/259 of query aligns to 2:258/258 of Q9ZNN8
- QGI 12:14 (≠ RGI 15:17) binding NAD(+)
- D33 (= D36) binding NAD(+)
- Q37 (≠ R38) binding NAD(+)
- DV 61:62 (= DV 62:63) binding NAD(+)
- N88 (= N89) binding NAD(+)
- I142 (= I143) mutation to Q: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with N-148.; mutation to Q: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
- F148 (= F149) mutation to N: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with Q-142.; mutation to N: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
- Y154 (= Y155) binding NAD(+)
- K158 (= K159) binding NAD(+)
- PGIVGT 184:189 (≠ PGVVGT 185:190) binding NAD(+)
3a28C Crystal structure of l-2,3-butanediol dehydrogenase (see paper)
52% identity, 98% coverage: 5:259/259 of query aligns to 1:257/257 of 3a28C
- active site: G12 (= G16), S140 (= S142), Y153 (= Y155), K157 (= K159), L198 (≠ F200)
- binding nicotinamide-adenine-dinucleotide: G8 (= G12), Q11 (≠ R15), I13 (= I17), D32 (= D36), L33 (= L37), Q36 (≠ R38), L59 (≠ A61), D60 (= D62), V61 (= V63), N87 (= N89), S140 (= S142), Y153 (= Y155), K157 (= K159), P183 (= P185), V186 (= V188), T188 (= T190), M190 (= M192), W191 (= W193)
6pejA Structure of sorbitol dehydrogenase from sinorhizobium meliloti 1021 bound to sorbitol
39% identity, 97% coverage: 4:255/259 of query aligns to 5:253/257 of 6pejA
3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
41% identity, 97% coverage: 6:256/259 of query aligns to 8:255/258 of 3ak4A
- active site: G18 (= G16), S141 (= S142), L151 (= L152), Y154 (= Y155), K158 (= K159), E199 (≠ F200)
- binding nicotinamide-adenine-dinucleotide: K17 (≠ R15), G18 (= G16), I19 (= I17), D38 (= D36), L39 (= L37), V60 (≠ A61), D61 (= D62), V62 (= V63), N88 (= N89), A89 (= A90), G90 (= G91), T139 (≠ A140), S141 (= S142), Y154 (= Y155), K158 (= K159), G185 (= G186), V187 (= V188), T189 (= T190), M191 (= M192)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
40% identity, 97% coverage: 6:256/259 of query aligns to 5:244/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
39% identity, 97% coverage: 6:256/259 of query aligns to 2:237/239 of 3sj7A
- active site: G12 (= G16), S138 (= S142), Q148 (≠ L152), Y151 (= Y155), K155 (= K159)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (≠ G14), R11 (= R15), I13 (= I17), N31 (≠ V35), Y32 (≠ D36), A33 (≠ L37), G34 (vs. gap), S35 (≠ R38), A58 (= A61), N59 (≠ D62), V60 (= V63), N86 (= N89), A87 (= A90), T109 (≠ V112), S138 (= S142), Y151 (= Y155), K155 (= K159), P181 (= P185), G182 (= G186)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
37% identity, 99% coverage: 1:257/259 of query aligns to 1:246/247 of 4jroC
- active site: G16 (= G16), S142 (= S142), Q152 (≠ L152), Y155 (= Y155), K159 (= K159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ G14), R15 (= R15), G16 (= G16), I17 (= I17), N35 (vs. gap), Y36 (≠ V35), N37 (≠ D36), G38 (≠ L37), S39 (≠ R38), N63 (≠ D62), V64 (= V63), N90 (= N89), A91 (= A90), I93 (= I92), I113 (≠ V112), S142 (= S142), Y155 (= Y155), K159 (= K159), P185 (= P185), I188 (≠ V188), T190 (= T190)
Q9L9F8 Short-chain reductase protein NovJ; Novobiocin biosynthesis protein J; EC 1.1.1.- from Streptomyces niveus (Streptomyces spheroides) (see paper)
40% identity, 96% coverage: 7:255/259 of query aligns to 18:260/262 of Q9L9F8
- S152 (= S142) mutation to A: 2-3-fold decrease in beta-ketotyrosine product formation.
- Y164 (= Y155) mutation to F: 50-fold reduction in catalytic activity.
- K168 (= K159) mutation to I: Does not alter the catalytic turnover.
6j7uA Crystal structure of blue fluorescent protein from metagenomic library in complex with NADPH (see paper)
38% identity, 97% coverage: 6:256/259 of query aligns to 6:245/247 of 6j7uA
- active site: G16 (= G16), S142 (= S142), Y156 (= Y155)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ G14), R15 (= R15), I17 (= I17), Y36 (≠ D36), V37 (≠ L37), S38 (≠ R38), S41 (vs. gap), D65 (= D62), S66 (≠ V63), N92 (= N89), A93 (= A90), G94 (= G91), I115 (≠ V112), G141 (≠ A141), S142 (= S142), Y156 (= Y155), K160 (= K159), P186 (= P185), T191 (= T190), M193 (= M192), N194 (≠ W193)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
36% identity, 97% coverage: 6:257/259 of query aligns to 8:243/244 of 4nbuB
- active site: G18 (= G16), N111 (= N113), S139 (= S142), Q149 (≠ L152), Y152 (= Y155), K156 (= K159)
- binding acetoacetyl-coenzyme a: D93 (≠ V95), K98 (≠ E100), S139 (= S142), N146 (≠ F149), V147 (≠ A150), Q149 (≠ L152), Y152 (= Y155), F184 (≠ V187), M189 (= M192), K200 (≠ L203)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), N17 (≠ R15), G18 (= G16), I19 (= I17), D38 (= D36), F39 (≠ L37), V59 (≠ A61), D60 (= D62), V61 (= V63), N87 (= N89), A88 (= A90), G89 (= G91), I90 (= I92), T137 (≠ A140), S139 (= S142), Y152 (= Y155), K156 (= K159), P182 (= P185), F184 (≠ V187), T185 (≠ V188), T187 (= T190), M189 (= M192)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
37% identity, 98% coverage: 1:255/259 of query aligns to 1:240/251 of H9XP47
- N15 (≠ R15) binding NAD(+)
- M17 (≠ I17) binding NAD(+)
- D36 (= D36) binding NAD(+)
- D60 (= D62) binding NAD(+)
- V61 (= V63) binding NAD(+)
- N87 (= N89) binding NAD(+)
- S138 (= S142) binding (R)-acetoin; binding (S)-acetoin
- V139 (≠ I143) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (≠ A144) binding (R)-acetoin
- Y151 (= Y155) binding (R)-acetoin; binding (S)-acetoin; binding NAD(+)
- K155 (= K159) binding NAD(+)
- V184 (= V188) binding NAD(+)
- T186 (= T190) binding NAD(+)
- RDK 197:199 (≠ DER 197:199) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
Sites not aligning to the query:
- 247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
6vspA Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
38% identity, 97% coverage: 4:255/259 of query aligns to 4:240/251 of 6vspA
- active site: G16 (= G16), S138 (= S142), Y151 (= Y155)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), N15 (≠ R15), G16 (= G16), M17 (≠ I17), D36 (= D36), W37 (≠ L37), W37 (≠ L37), A38 (≠ R38), I59 (≠ A61), D60 (= D62), V61 (= V63), N87 (= N89), A88 (= A90), G89 (= G91), V90 (≠ I92), V110 (= V112), T136 (≠ A140), S138 (= S142), Y151 (= Y155), K155 (= K159), P181 (= P185), S182 (≠ G186), L183 (≠ V187), V184 (= V188), T186 (= T190), N187 (≠ D191), M188 (= M192), T189 (vs. gap)
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
38% identity, 97% coverage: 4:255/259 of query aligns to 6:242/252 of 6vspB
6xewA Structure of serratia marcescens 2,3-butanediol dehydrogenase (see paper)
38% identity, 97% coverage: 4:255/259 of query aligns to 4:240/251 of 6xewA