SitesBLAST
Comparing WP_058858058.1 NCBI__GCF_001482365.1:WP_058858058.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
36% identity, 91% coverage: 15:333/350 of query aligns to 7:336/362 of 3bptA
- active site: G67 (= G75), P84 (≠ T93), R88 (≠ A97), G115 (= G124), G118 (= G127), E138 (= E147), D146 (= D155)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G74), G67 (= G75), I69 (= I77), E90 (= E99), G114 (= G123), G115 (= G124), E138 (= E147), D146 (= D155), V147 (≠ I156)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ Q33), L26 (= L34), A28 (= A36), G66 (= G74), G67 (= G75), I69 (= I77), P137 (= P146), I141 (= I150), L319 (= L316)
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 95% coverage: 12:343/350 of query aligns to 8:354/378 of Q9LKJ1
- G70 (= G75) mutation to S: Loss of activity.
- E142 (= E147) mutation to A: Loss of activity.
- D150 (= D155) mutation to G: Reduced activity.
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
37% identity, 94% coverage: 15:342/350 of query aligns to 4:328/340 of 4hdtA
- active site: G64 (= G75), I69 (≠ V80), W84 (≠ A97), Y88 (= Y100), G112 (= G124), G115 (= G127), E135 (= E147), P142 (≠ T154), D143 (= D155), R283 (≠ G293)
- binding zinc ion: H28 (≠ A39), E42 (≠ R53), E57 (≠ P68), E79 (≠ A92), H93 (≠ L105), H185 (≠ D206)
Sites not aligning to the query:
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
31% identity, 61% coverage: 15:226/350 of query aligns to 5:205/257 of 6slbAAA
- active site: Q64 (≠ G75), F69 (≠ V80), L80 (≠ I89), N84 (≠ T93), A108 (≠ G124), S111 (≠ G127), A130 (≠ P146), F131 (≠ E147), L136 (≠ F152), P138 (≠ T154), D139 (= D155)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K69), A62 (= A73), Q64 (≠ G75), D65 (= D76), L66 (≠ I77), Y76 (≠ T85), A108 (≠ G124), F131 (≠ E147), D139 (= D155)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
27% identity, 56% coverage: 26:221/350 of query aligns to 17:203/259 of 5zaiC
- active site: A65 (≠ G75), F70 (≠ V80), S82 (≠ A92), R86 (≠ F96), G110 (= G124), E113 (≠ G127), P132 (= P146), E133 (= E147), I138 (≠ F152), P140 (≠ T154), G141 (≠ D155)
- binding coenzyme a: K24 (≠ Q33), L25 (= L34), A63 (= A73), G64 (= G74), A65 (≠ G75), D66 (= D76), I67 (= I77), P132 (= P146), R166 (= R188)
Sites not aligning to the query:
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
31% identity, 61% coverage: 15:226/350 of query aligns to 2:193/245 of 6slaAAA
- active site: Q61 (≠ G75), L68 (≠ I89), N72 (≠ T93), A96 (≠ G124), S99 (≠ G127), A118 (≠ P146), F119 (≠ E147), L124 (≠ F152), P126 (≠ T154), N127 (≠ D155)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L34), A59 (= A73), Q61 (≠ G75), D62 (= D76), L63 (≠ I77), L68 (≠ I89), Y71 (≠ A92), A94 (≠ M122), G95 (= G123), A96 (≠ G124), F119 (≠ E147), I122 (= I150), L124 (≠ F152), N127 (≠ D155)
Sites not aligning to the query:
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
28% identity, 49% coverage: 33:204/350 of query aligns to 24:186/707 of 6yswA
- active site: A66 (≠ G75), I71 (≠ V80), A84 (≠ T93), Q88 (≠ Y100), G112 (= G124), E115 (vs. gap), P136 (= P146), E137 (= E147), G145 (≠ D155)
- binding coenzyme a: M25 (≠ L34), A66 (≠ G75), D67 (= D76), I68 (= I77), P136 (= P146), E137 (= E147), L140 (≠ I150)
Sites not aligning to the query:
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
29% identity, 53% coverage: 14:199/350 of query aligns to 4:172/254 of 2dubA
- active site: A67 (≠ G75), M72 (≠ V80), S82 (≠ A101), G105 (= G124), E108 (≠ G127), P127 (= P146), E128 (= E147), T133 (≠ F152), P135 (≠ T154), G136 (≠ D155)
- binding octanoyl-coenzyme a: K25 (≠ R32), A26 (≠ Q33), L27 (= L34), A29 (= A36), A65 (= A73), A67 (≠ G75), D68 (= D76), I69 (= I77), K70 (≠ R78), G105 (= G124), E108 (≠ G127), P127 (= P146), E128 (= E147), G136 (≠ D155), A137 (≠ I156)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
29% identity, 53% coverage: 14:199/350 of query aligns to 35:208/290 of P14604
- E144 (≠ G127) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E147) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
29% identity, 53% coverage: 14:199/350 of query aligns to 3:176/258 of 1ey3A
- active site: A66 (≠ G75), M71 (≠ V80), S81 (≠ A101), L85 (= L105), G109 (= G124), E112 (≠ G127), P131 (= P146), E132 (= E147), T137 (≠ F152), P139 (≠ T154), G140 (≠ D155)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ R32), L26 (= L34), A28 (= A36), A64 (= A73), G65 (= G74), A66 (≠ G75), D67 (= D76), I68 (= I77), L85 (= L105), W88 (vs. gap), G109 (= G124), P131 (= P146), L135 (≠ I150), G140 (≠ D155)
Sites not aligning to the query:
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
29% identity, 53% coverage: 14:199/350 of query aligns to 5:178/260 of 1dubA
- active site: A68 (≠ G75), M73 (≠ V80), S83 (≠ A101), L87 (= L105), G111 (= G124), E114 (≠ G127), P133 (= P146), E134 (= E147), T139 (≠ F152), P141 (≠ T154), G142 (≠ D155)
- binding acetoacetyl-coenzyme a: K26 (≠ R32), A27 (≠ Q33), L28 (= L34), A30 (= A36), A66 (= A73), A68 (≠ G75), D69 (= D76), I70 (= I77), Y107 (≠ I120), G110 (= G123), G111 (= G124), E114 (≠ G127), P133 (= P146), E134 (= E147), L137 (≠ I150), G142 (≠ D155)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
28% identity, 53% coverage: 14:199/350 of query aligns to 5:176/258 of 1mj3A
- active site: A68 (≠ G75), M73 (≠ V80), S83 (vs. gap), L85 (vs. gap), G109 (= G124), E112 (≠ G127), P131 (= P146), E132 (= E147), T137 (≠ F152), P139 (≠ T154), G140 (≠ D155)
- binding hexanoyl-coenzyme a: K26 (≠ R32), A27 (≠ Q33), L28 (= L34), A30 (= A36), A66 (= A73), G67 (= G74), A68 (≠ G75), D69 (= D76), I70 (= I77), G109 (= G124), P131 (= P146), E132 (= E147), L135 (≠ I150), G140 (≠ D155)
Sites not aligning to the query:
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
30% identity, 53% coverage: 14:199/350 of query aligns to 5:178/260 of 2hw5C
- active site: A68 (≠ G75), M73 (≠ V80), S83 (≠ A101), L87 (= L105), G111 (= G124), E114 (≠ G127), P133 (= P146), E134 (= E147), T139 (≠ F152), P141 (≠ T154), G142 (≠ D155)
- binding crotonyl coenzyme a: K26 (≠ R32), A27 (≠ Q33), L28 (= L34), A30 (= A36), K62 (= K69), I70 (= I77), F109 (≠ M122)
Sites not aligning to the query:
7borA Structure of pseudomonas aeruginosa coa-bound odaa (see paper)
32% identity, 64% coverage: 16:239/350 of query aligns to 3:204/247 of 7borA
- active site: N63 (≠ G75), F68 (≠ V80), D77 (≠ I89), G81 (≠ T93), I105 (≠ G124), T108 (≠ G127), F128 (≠ E147), L133 (≠ F152), P135 (≠ T154), E136 (≠ D155)
- binding coenzyme a: D21 (vs. gap), K22 (vs. gap), A25 (= A36), S61 (≠ A73), I65 (= I77), V103 (≠ M122), F128 (≠ E147), L131 (≠ I150)
Sites not aligning to the query:
Q9P4U9 Enoyl-CoA hydratase AKT3-1; AF-toxin biosynthesis protein 3-1; EC 4.2.1.17 from Alternaria alternata (Alternaria rot fungus) (Torula alternata) (see paper)
30% identity, 43% coverage: 21:170/350 of query aligns to 23:167/296 of Q9P4U9
Sites not aligning to the query:
- 294:296 Peroxisomal targeting signal type 1
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
28% identity, 42% coverage: 18:165/350 of query aligns to 5:142/692 of 6iunB
Sites not aligning to the query:
- active site: 248, 407, 428, 440, 478
- binding nicotinamide-adenine-dinucleotide: 300, 301, 302, 321, 322, 365, 377, 378, 380, 384, 388, 405, 407
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
26% identity, 62% coverage: 15:231/350 of query aligns to 5:208/256 of 3h81A
- active site: A64 (≠ G75), M69 (≠ V80), T79 (≠ A101), F83 (≠ L105), G107 (= G124), E110 (≠ G127), P129 (= P146), E130 (= E147), V135 (≠ F152), P137 (≠ T154), G138 (≠ D155)
Sites not aligning to the query:
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
26% identity, 62% coverage: 15:231/350 of query aligns to 6:209/255 of 3q0jC
- active site: A65 (≠ G75), M70 (≠ V80), T80 (≠ A101), F84 (≠ L105), G108 (= G124), E111 (≠ G127), P130 (= P146), E131 (= E147), V136 (≠ F152), P138 (≠ T154), G139 (≠ D155)
- binding acetoacetyl-coenzyme a: Q23 (≠ R32), A24 (≠ Q33), L25 (= L34), A27 (= A36), A63 (= A73), G64 (= G74), A65 (≠ G75), D66 (= D76), I67 (= I77), K68 (≠ R78), M70 (≠ V80), F84 (≠ L105), G107 (= G123), G108 (= G124), E111 (≠ G127), P130 (= P146), E131 (= E147), P138 (≠ T154), G139 (≠ D155), M140 (≠ I156)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
26% identity, 62% coverage: 15:231/350 of query aligns to 6:209/255 of 3q0gC