SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_058858531.1 NCBI__GCF_001482365.1:WP_058858531.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

6r6uA Crystal structure of human cis-aconitate decarboxylase (see paper)
23% identity, 77% coverage: 94:482/506 of query aligns to 72:445/458 of 6r6uA

query
sites
6r6uA

K

R

V

L

S

P

P

P

E

T

W

Y

A

A

A

A

W

F

A

V

N

N

G

G

V

V

A

A

V

I

R

H

E

S

L

M

D

D

Y

F

H

D

D

D

T

T

F

W

L

H

A

P

A

A

E

-

Y

-

S

T

H

H

P

P

G

S

D

G

N

A

I

V

P

L

P

P

I

V

L

L

-

T

-

A

-

L

-

A

-

E

A

A

V

L

A

P

Q

R

H

S

A

P

G

K

A

F

S

S

G

G

A

L

D

D

L

L

V

L

R

L

G

A

I

F

A

N

T

V

G

G

Y

I

E

E

L

V

Q

Q

V

G

D

R

L

L

V

L

-

H

-

F

-

A

-

K

-

E

-
x
A

K

N

S

D

I

M

C

P

L

K

H

R

K
x
F

H

H

K

P

I

P

D

S

H

V

V

V

A

G

H

T

L

L

G

G

P

S

S

A

A

A

A

A

A

A

G

-

I

-

G

S

T

K

L

F

L

L

G

G

L

L

D

S

V

S

P

T

T

K

I

C

F

R

H

E

A

A

I

L

G

A

-

I

-

A

-

V

-

S

-

H

Q

A

G

G

L

A

H

P

T

M

T

A

T

N

A

A

T

A

R

T

Q

Q

S

T

R

K

K

P

G

L

E

H

I

I

S

G

T

N

W

-

K

-

A

-

H

-

A

-

P

-

A

-

F

-

A

A

G

A

K

K

M

H

A

G

V

I

E

E

A

A

V

A

D

F

R

L

A

A

M

M

R

L

G

G

Q

L

T

Q

S

G

P

N

T

K

P

Q

I

V

Y

L

E

D

G

L

E

E

D

A

G

G

V

F

-

G

-

A

-
x
F

-
x
Y

-

A

-

N

-

Y

-

S

-

P

-

K

-

V

-

L

-

P

-

S

-

I

-

A

-

S

I

Y

A

S

W

W

L

L

L

L

D

D

G

Q

P

Q

G

D

A

V

C

A

Y

F

E

-

V

-

P

-

L

-

P

-

A

-

P

-

G

-

E

-

A

-

K

K

R

R

A

-

I

-

L

-

D

-

T

-

Y

F

T

P

K

A

E

H

H

L

S

S

A

T

E

H

Y

W

Q

V

A

A

Q

D

A

A

W

A

I

A

D

S

L

V

A

R

R

K

K

H

L

L

H

V

R

A

E

E

R

R

P

-

E

A

L

L

A

L

D

P

P

T

S

D

R

Y

I

I

E

K

R

R

I

I

V

V

L

L

H

R

T

I

S

P

H

-

H

N

T

V

H

Q

Y

Y

V

V

I

-

G

-

S

-

G

-

A

-

G

-

D

-

P

-

Q

N

K

R

Y

P

D

F

P

P

A

V

A

S

T

E

R

H

E

E

T

A

L

-

D

R

H

H

S

S

I

F

P

Q

Y

Y

I

V

F

A

T

C

V

A

A

M

L

L

Q

L

D

D

G

G

A

G

W

I

H

-

H

T

V

V

D

P

S

S

Y

F

A

H

P

E

E

C

R

Q

A

I

G

N

R

R

P

P

D

Q

T

V

V

R

S

E

L

L

W

L

H

S

K

K

V

V

T

E

T

L

A

E

E

Y

D

P

A

P

E

D

W

N

T

L

R

-

R

-

Y

-

H

-

S

-

L

-

D

-

P

P

Q

S

E

F

K

N

A

I

F

L

G

Y

G

C

R

E

A

I

E

S

I

V

V

T

L

L

S

K

D

D

G

G

S

A

T

T

V

F

V

T

E

D

E

R

I

S

A

D

V

T

A

F

D

Y

A

G

H

H

P

-

L

-

G

W

A

R

R

K

P

P

F

L

A

S

R

Q

E

E

Q

D

Y

L

V

E

A

E

K

K

L

F

R

R

T

A

L

N

A

A

E

S

G

K

I

M

V

L

E

S

D

W

A

D

E

T

L

V

D

E

R

S

F

L

I

I

A

K

A

I

A

V

E

K

N

N

L

L

T

E

S

D

L

L

binding (r,r)-2,3-butanediol: A148 (vs. gap), F155 (≠ K168), F242 (vs. gap), Y243 (vs. gap)

A6NK06 Cis-aconitate decarboxylase; CAD; Aconitate decarboxylase; Aconitate decarboxylase 1; Cis-aconitic acid decarboxylase; Immune-responsive gene 1 protein; EC 4.1.1.6 from Homo sapiens (Human) (see paper)
23% identity, 77% coverage: 94:482/506 of query aligns to 75:448/481 of A6NK06

query
sites
A6NK06

K

R

V

L

S

P

P

P

E

T

W

Y

A

A

A

A

W

F

A

V

N

N

G

G

V

V

A

A

V

I

R

H

E

S

L

M

D
|
D

Y

F

H

D

D

D

T
|
T

F

W

L

H

A

P

A

A

E

-

Y

-

S

T

H
|
H

P

P

G

S

D

G

N

A

I

V

P

L

P

P

I

V

L

L

-

T

-

A

-

L

-

A

-

E

A

A

V

L

A

P

Q

R

H

S

A

P

G

K

A

F

S

S

G

G

A

L

D

D

L

L

V

L

R

L

G

A

I

F

A

N

T

V

G

G

Y

I

E

E

L

V

Q

Q

V

G

D

R

L

L

V

L

-

H

-

F

-

A

-

K

-

E

-

A

K
x
N

S

D

I

M

C

P

L

K

H

R

K

F

H
|
H

K

P

I

P

D

S

H

V

V

V

A

G

H

T

L

L

G

G

P

S

S

A

A

A

A

A

A

A

G

-

I

-

G

S

T

K

L

F

L

L

G

G

L

L

D

S

V

S

P

T

T

K

I

C

F

R

H

E

A

A

I

L

G

A

-

I

-

A

-

V

-

S

-

H

Q

A

G

G

L

A

H

P

T

M

T

A

T

N

A

A

T

A

R

T

Q

Q

S

T

R
x
K

K

P

G

L

E

H

I

I

S

G

T

N

W

-

K

-

A

-

H

-

A

-

P

-

A

-

F

-

A

A

G

A

K

K

M

H

A

G

V

I

E

E

A

A

V

A

D

F

R

L

A

A

M

M

R

L

G

G

Q

L

T

Q

S

G

P

N

T

K

P

Q

I

V

Y

L

E

D

G

L

E

E

D

A

G

G

V

F

-

G

-

A

-

F

-

Y

-

A

-

N

-

Y

-

S

-

P

-

K

-

V

-

L

-

P

-

S

-

I

-

A

-

S

I

Y

A

S

W

W

L

L

L

L

D

D

G

Q

P

Q

G

D

A

V

C

A

Y

F

E

-

V

-

P

-

L

-

P

-

A

-

P

-

G

-

E

-

A

-

K
|
K

R
|
R

A

-

I

-

L

-

D

-

T

-

Y

F

T

P

K

A

E
x
H

H

L

S

S

A

T

E

H

Y

W

Q

V

A

A

Q

D

A

A

W

A

I

A

D

S

L

V

A

R

R

K

K

H

L

L

H

V

R

A

E

E

R

R

P

-

E

A

L

L

A

L

D

P

P

T

S

D

R

Y

I

I

E

K

R

R

I

I

V

V

L

L

H

R

T

I

S

P

H

-

H

N

T

V

H

Q

Y
|
Y

V

V

I

-

G

-

S

-

G

-

A

-

G

-

D

-

P

-

Q

N

K

R

Y

P

D

F

P

P

A

V

A

S

T

E

R

H

E

E

T

A

L

-

D
x
R

H

H

S

S

I

F

P

Q

Y

Y

I

V

F

A

T

C

V

A

A

M

L

L

Q

L

D

D

G

G

A

G

W

I

H

-

H

T

V

V

D

P

S

S

Y

F

A

H

P

E

E

C

R

Q

A

I

G

N

R

R

P

P

D

Q

T

V

V

R

S

E

L

L

W

L

H

S

K

K

V

V

T

E

T

L

A

E

E

Y

D

P

A

P

E

D

W

N

T

L

R

-

R

-

Y

-

H

-

S

-

L

-

D

-

P

P

Q

S

E

F

K

N

A

I

F

L

G

Y

G

C

R

E

A

I

E

S

I

V

V

T

L

L

S

K

D

D

G

G

S

A

T

T

V

F

V

T

E

D

E

R

I

S

A

D

V

T

A

F

D

Y

A

G

H

H

P

-

L

-

G

W

A

R

R

K

P

P

F

L

A

S

R

Q

E

E

Q

D

Y

L

V

E

A

E

K

K

L

F

R

R

T

A

L

N

A

A

E

S

G

K

I

M

V

L

E

S

D

W

A

D

E

T

L

V

D

E

R

S

F

L

I

I

A

K

A

I

A

V

E

K

N

N

L

L

T

E

S

D

L

L

D93 (= D112) mutation to A: Abolished cis-aconitate decarboxylase activity.
T97 (= T116) to M: strongly reduced cis-aconitate decarboxylase activity; dbSNP:rs767323284; mutation to A: Strongly reduced cis-aconitate decarboxylase activity.
H103 (= H124) mutation to A: Abolished cis-aconitate decarboxylase activity.
N152 (≠ K162) to S: increased cis-aconitate decarboxylase activity
H159 (= H169) to Q: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; to R: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs1471882722; mutation to A: Abolished cis-aconitate decarboxylase activity.
K207 (≠ R214) mutation to A: Abolished cis-aconitate decarboxylase activity.
K272 (= K280) to Q: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs1018207074; mutation to A: Abolished cis-aconitate decarboxylase activity.
R273 (= R281) to H: does not affect cis-aconitate decarboxylase activity
H277 (≠ E290) to Y: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs1289708092; mutation to A: Abolished cis-aconitate decarboxylase activity.
Y318 (= Y333) mutation to A: Abolished cis-aconitate decarboxylase activity.
R331 (≠ D355) to H: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs755737247

Query Sequence

>WP_058858531.1 NCBI__GCF_001482365.1:WP_058858531.1
MTVLHTVRVHPSADNLPREEQLAWKMAEVAADPVEVTPEVTEMVINRILDNASVAIASLD
RGPIVSARDQALAHAATPGGTGSALFGVHPGERKVSPEWAAWANGVAVRELDYHDTFLAA
EYSHPGDNIPPILAVAQHAGASGADLVRGIATGYELQVDLVKSICLHKHKIDHVAHLGPS
AAAGIGTLLGLDVPTIFHAIGQGLHTTTATRQSRKGEISTWKAHAPAFAGKMAVEAVDRA
MRGQTSPTPIYEGEDGVIAWLLDGPGACYEVPLPAPGEAKRAILDTYTKEHSAEYQAQAW
IDLARKLHRERPELADPSRIERIVLHTSHHTHYVIGSGAGDPQKYDPAATRETLDHSIPY
IFTVALQDGAWHHVDSYAPERAGRPDTVSLWHKVTTAEDAEWTRRYHSLDPQEKAFGGRA
EIVLSDGSTVVEEIAVADAHPLGARPFAREQYVAKLRTLAEGIVEDAELDRFIAAAENLT
SLGAGELDALNVTAARTLRTGTKGVF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory