SitesBLAST
Comparing WP_058858667.1 NCBI__GCF_001482365.1:WP_058858667.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
50% identity, 97% coverage: 13:405/407 of query aligns to 11:391/394 of 1phpA
- active site: R36 (= R38), K197 (= K206), G351 (= G364), G374 (= G388)
- binding adenosine-5'-diphosphate: G195 (= G204), K201 (= K210), G219 (= G228), G220 (= G229), L237 (= L246), N316 (= N330), P318 (= P332), G320 (= G334), V321 (= V335), E323 (= E337), G350 (= G363), D352 (= D365), S353 (= S366)
P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
50% identity, 97% coverage: 13:405/407 of query aligns to 11:391/394 of P18912
P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
48% identity, 97% coverage: 13:405/407 of query aligns to 11:394/654 of P36204
- R36 (= R38) binding substrate
- R118 (= R120) binding substrate
- R151 (= R160) binding substrate
1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
48% identity, 97% coverage: 13:405/407 of query aligns to 10:393/398 of 1vpeA
- active site: R35 (= R38), K196 (= K206), G353 (= G364), G376 (= G388)
- binding phosphoaminophosphonic acid-adenylate ester: G194 (= G204), A195 (≠ S205), K196 (= K206), K200 (= K210), G218 (= G228), A219 (≠ G229), N316 (= N330), P318 (= P332), G320 (= G334), V321 (= V335), E323 (= E337), G352 (= G363), G353 (= G364), D354 (= D365), S355 (= S366)
P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
49% identity, 97% coverage: 13:406/407 of query aligns to 11:392/394 of P40924
- S183 (≠ Q192) modified: Phosphoserine
- T299 (≠ S313) modified: Phosphothreonine
3zlbA Crystal structure of phosphoglycerate kinase from streptococcus pneumoniae (see paper)
45% identity, 97% coverage: 13:405/407 of query aligns to 11:395/398 of 3zlbA
- active site: R36 (= R38), K204 (= K206), G355 (= G364), G378 (= G388)
- binding phosphoaminophosphonic acid-adenylate ester: G202 (= G204), S203 (= S205), G226 (= G228), G227 (= G229), N320 (= N330), P322 (= P332), G324 (= G334), V325 (= V335), E327 (= E337), G354 (= G363), G355 (= G364), D356 (= D365), S357 (= S366)
Sites not aligning to the query:
P00560 Phosphoglycerate kinase; EC 2.7.2.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
41% identity, 96% coverage: 16:405/407 of query aligns to 17:412/416 of P00560
- R22 (= R21) mutation to K: 2-fold reduction of Vmax.; mutation to M: 7-fold reduction of Vmax.
- R39 (= R38) binding substrate
- R122 (= R120) binding substrate
- R169 (= R160) binding substrate
1qpgA 3-phosphoglycerate kinase, mutation r65q (see paper)
41% identity, 96% coverage: 16:405/407 of query aligns to 16:411/415 of 1qpgA
- active site: R38 (= R38), K213 (= K206), G371 (= G364), G394 (= G388)
- binding magnesium-5'-adenyly-imido-triphosphate: G235 (= G228), G236 (= G229), N334 (= N330), P336 (= P332), G338 (= G334), V339 (= V335), F340 (= F336), E341 (= E337), G370 (= G363), G371 (= G364), D372 (= D365), T373 (≠ S366)
O60101 Phosphoglycerate kinase; EC 2.7.2.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
40% identity, 97% coverage: 13:407/407 of query aligns to 14:413/414 of O60101
- Y75 (= Y73) modified: Phosphotyrosine
- S76 (= S74) modified: Phosphoserine
- S143 (≠ G134) modified: Phosphoserine
- S172 (≠ A163) modified: Phosphoserine
- S173 (= S164) modified: Phosphoserine
- S183 (≠ Q176) modified: Phosphoserine
- S253 (= S245) modified: Phosphoserine
- S260 (≠ L252) modified: Phosphoserine
- T299 (≠ P291) modified: Phosphothreonine
- S328 (≠ A324) modified: Phosphoserine
- S351 (≠ A347) modified: Phosphoserine
- T373 (≠ S366) modified: Phosphothreonine
- S387 (≠ G381) modified: Phosphoserine
- S390 (= S384) modified: Phosphoserine
- S412 (≠ G406) modified: Phosphoserine
- S413 (≠ A407) modified: Phosphoserine
16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
40% identity, 97% coverage: 13:405/407 of query aligns to 10:412/415 of 16pkA
- active site: R35 (= R38), K215 (= K206), G372 (= G364), G395 (= G388)
- binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G204), A214 (≠ S205), K219 (= K210), A238 (≠ G229), Y241 (≠ F232), L311 (= L307), P336 (= P332), G338 (= G334), V339 (= V335), E341 (= E337), G393 (= G386), G394 (= G387), G395 (= G388)
13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
40% identity, 97% coverage: 13:405/407 of query aligns to 10:412/415 of 13pkA
- active site: R35 (= R38), K215 (= K206), G372 (= G364), G395 (= G388)
- binding adenosine-5'-diphosphate: G213 (= G204), A214 (≠ S205), K219 (= K210), L311 (= L307), P336 (= P332), G338 (= G334), V339 (= V335), E341 (= E337), G371 (= G363), D373 (= D365), S374 (= S366)
4axxA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp 3-phosphoglycerate and beryllium trifluoride
40% identity, 97% coverage: 13:405/407 of query aligns to 12:404/407 of 4axxA