SitesBLAST
Comparing WP_058929091.1 NCBI__GCF_001484605.1:WP_058929091.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 14 hits to proteins with known functional sites (download)
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
33% identity, 98% coverage: 1:253/257 of query aligns to 1:254/262 of 6fahB
- binding flavin-adenine dinucleotide: C6 (≠ L6), A7 (≠ V7), K8 (= K8), D38 (≠ N39), T61 (= T62), M62 (= M63), A93 (= A94), D94 (= D95), T95 (≠ I96), T98 (= T99), L102 (= L103), G118 (= G118), Q120 (≠ E120), G124 (= G124), T126 (≠ G126), A127 (≠ G127), V129 (= V129), T223 (≠ S220)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
34% identity, 100% coverage: 1:257/257 of query aligns to 1:261/263 of 4l2iB
- binding flavin-adenine dinucleotide: C6 (≠ L6), V7 (= V7), K8 (= K8), Y37 (≠ I38), D38 (≠ N39), Y40 (≠ R41), T60 (= T62), M61 (= M63), A92 (= A94), D93 (= D95), T94 (≠ I96), T97 (= T99), M101 (≠ L103), C116 (≠ A117), G117 (= G118), Q119 (≠ E120), A120 (≠ S121), G123 (= G124), T125 (≠ G126), A126 (≠ G127), V128 (= V129)
- binding nicotinamide-adenine-dinucleotide: M61 (= M63), G62 (= G64), P63 (= P65), S85 (≠ E87), D86 (= D88), G91 (= G93)
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
34% identity, 100% coverage: 1:257/257 of query aligns to 1:261/263 of 4kpuB
- binding flavin-adenine dinucleotide: C6 (≠ L6), V7 (= V7), K8 (= K8), Y37 (≠ I38), D38 (≠ N39), Y40 (≠ R41), M61 (= M63), A92 (= A94), D93 (= D95), T94 (≠ I96), T97 (= T99), C116 (≠ A117), G117 (= G118), Q119 (≠ E120), A120 (≠ S121), G123 (= G124), T125 (≠ G126), A126 (≠ G127), V128 (= V129)
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
31% identity, 91% coverage: 1:234/257 of query aligns to 1:236/260 of 5ol2B
- binding flavin-adenine dinucleotide: C6 (≠ L6), I7 (≠ V7), K8 (= K8), D38 (≠ N39), M61 (= M63), A92 (= A94), D93 (= D95), T94 (≠ I96), T97 (= T99), A115 (= A117), G116 (= G118), A119 (≠ S121), G122 (= G124), T124 (≠ G126), A125 (≠ G127), V127 (= V129)
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
31% identity, 97% coverage: 3:251/257 of query aligns to 4:264/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (≠ L6), I8 (≠ V7), K9 (= K8), D39 (≠ N39), M62 (= M63), A93 (= A94), D94 (= D95), T95 (≠ I96), T98 (= T99), L102 (= L103), T121 (≠ A117), G122 (= G118), A125 (≠ S121), G128 (= G124), T130 (≠ G126), G131 (= G127), V133 (= V129)
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
31% identity, 81% coverage: 2:209/257 of query aligns to 3:211/265 of 7qh2B
- binding flavin-adenine dinucleotide: C7 (≠ L6), K9 (= K8), D39 (≠ N39), M62 (= M63), D94 (= D95), V95 (≠ I96), L102 (= L103), G118 (= G118), Q120 (≠ E120), T121 (≠ S121), T126 (≠ G126), A127 (≠ G127), V129 (= V129)
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
29% identity, 92% coverage: 1:237/257 of query aligns to 1:236/246 of 1efpB
- binding adenosine monophosphate: P6 (≠ L6), V7 (= V7), D39 (≠ N39), I63 (≠ M63), V101 (≠ T99), A119 (= A117), G120 (= G118), Q122 (≠ E120), A123 (≠ S121), N126 (≠ G124), M128 (≠ G126), N129 (≠ G127), T131 (≠ V129)
- binding flavin-adenine dinucleotide: Y13 (≠ T13), L182 (≠ E180)
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
31% identity, 92% coverage: 1:237/257 of query aligns to 1:236/252 of 2a1uB
- binding adenosine monophosphate: A6 (≠ L6), V7 (= V7), K8 (= K8), C39 (≠ N39), C63 (≠ M63), V101 (≠ T99), L119 (≠ A117), G120 (= G118), Q122 (≠ E120), A123 (≠ S121), D126 (≠ G124), C128 (≠ G126), N129 (≠ G127), Q130 (≠ V128), T131 (≠ V129)
- binding flavin-adenine dinucleotide: P37 (≠ E37), L182 (≠ E180)
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
31% identity, 92% coverage: 1:237/257 of query aligns to 4:239/255 of P38117
- A9 (≠ L6) binding AMP
- NPFC 39:42 (≠ DEIN 36:39) binding AMP
- C66 (≠ M63) binding AMP
- 123:134 (vs. 118:129, 17% identical) binding AMP
- D128 (= D123) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ E149) to M: in dbSNP:rs1130426
- R164 (≠ V159) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (≠ T160) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
- 183:205 (vs. 178:200, 35% identical) Recognition loop
- L195 (≠ F190) mutation to A: Severely impaired in complex formation with ACADM.
- K200 (≠ T195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- KAK 200:202 (≠ TAK 195:197) mutation to RAR: Does not abolish methylation by ETFBKMT.
- KAKK 200:203 (≠ TAKR 195:198) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- K203 (≠ R198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
30% identity, 92% coverage: 1:237/257 of query aligns to 4:239/255 of Q2TBV3
- K200 (≠ T195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (≠ R198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
29% identity, 92% coverage: 1:237/257 of query aligns to 4:239/255 of Q9DCW4
- K200 (≠ T195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (≠ R198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
28% identity, 98% coverage: 1:251/257 of query aligns to 1:255/264 of P53570
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
29% identity, 98% coverage: 1:251/257 of query aligns to 1:254/261 of 1o96A
- binding adenosine monophosphate: A6 (≠ L6), V7 (= V7), K8 (= K8), D38 (≠ N39), V63 (≠ M63), V99 (≠ T99), A117 (= A117), G118 (= G118), Q120 (≠ E120), S121 (= S121), Y126 (≠ G126), A127 (≠ G127), T129 (≠ V129)
- binding flavin-adenine dinucleotide: Q120 (≠ E120), Q182 (≠ T179)
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
33% identity, 79% coverage: 1:204/257 of query aligns to 1:202/251 of 5ow0B
- binding adenosine monophosphate: L6 (= L6), A7 (≠ V7), K8 (= K8), D39 (= D27), Y62 (≠ M63), I95 (≠ T99), A114 (= A117), G115 (= G118), Q117 (≠ E120), S118 (= S121), R123 (≠ G126), G124 (= G127), V125 (= V128), V126 (= V129), P127 (= P130)
- binding flavin-adenine dinucleotide: L37 (≠ V25), Y38 (≠ L26)
Query Sequence
>WP_058929091.1 NCBI__GCF_001484605.1:WP_058929091.1
MKIIVLVKQVPDTEEERRLDSVTGVLDRDASESVADEINERALEVALRHKDANKGSEVVV
LTMGPASATQALRKALSMGADSAVHVEDDRLEGADIATTAAVLAAAVQRTGFDLVVAGNE
STDGRGGVVPAMMAEHLGLPLLSSLNTLELTGGTVAGEVTVESGSLAVSAALPAIVSVTE
RSAEARFPNFKGIMTAKRKPLATLSLTDLGLDAGVTGGTSTVLTISERPARTAGKKIVDD
GTAARELAEFLVAGRLI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory