SitesBLAST
Comparing WP_058929120.1 NCBI__GCF_001484605.1:WP_058929120.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2d3tC Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form v (see paper)
41% identity, 99% coverage: 1:383/385 of query aligns to 2:388/390 of 2d3tC
- active site: C94 (= C94), H346 (= H341), C376 (= C371), G378 (= G373)
- binding acetyl coenzyme *a: C94 (= C94), R214 (= R213), L222 (= L221), L225 (= L224), A238 (= A236), G239 (= G237), S242 (= S240), I244 (≠ L242), A313 (= A311), F314 (= F312), H346 (= H341), C376 (= C371)
4ubvA Structure of the 3-ketoacyl-coa thiolase fada5 from m. Tuberculosis with an partially acetylated cysteine in complex with acetyl-coa and coa (see paper)
44% identity, 98% coverage: 7:385/385 of query aligns to 5:391/391 of 4ubvA
- active site: C93 (= C94), H347 (= H341), C377 (= C371), G379 (= G373)
- binding acetyl coenzyme *a: C93 (= C94), Q151 (= Q154), F152 (≠ G155), R221 (= R213), T223 (≠ S216), L231 (= L224), A242 (= A236), S246 (= S240), I248 (≠ L242), A317 (= A311), H347 (= H341)
- binding coenzyme a: L128 (≠ M129), F152 (≠ G155), R221 (= R213), T223 (≠ S216), L231 (= L224), A242 (= A236), I248 (≠ L242)
I6XHI4 Steroid 3-ketoacyl-CoA thiolase; Acetyl-CoA acetyltransferase FadA5; Beta-ketoacyl-CoA thiolase; EC 2.3.1.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 98% coverage: 7:385/385 of query aligns to 5:391/391 of I6XHI4
- Q151 (= Q154) binding CoA
- RE-T 221:223 (≠ RPGS 213:216) binding CoA
- S246 (= S240) binding CoA
- G379 (= G373) binding substrate
8gqmA Crystal structure of thiolase complexed with acetyl coenzyme a
43% identity, 94% coverage: 22:383/385 of query aligns to 17:373/377 of 8gqmA
- binding acetyl coenzyme *a: K18 (= K23), S89 (≠ C94), M124 (= M129), M146 (≠ Q154), R205 (= R213), T208 (≠ S216), L213 (= L221), L216 (= L224), A226 (= A236), A227 (≠ G237), S229 (≠ A239), S230 (= S240), M271 (= M281), A301 (= A311), H331 (= H341), L333 (= L343)
4ubtA Structure of the c93s variant of the 3-ketoacyl-coa thiolase fada5 from m. Tuberculosis in complex with a steroid and coa. (see paper)
44% identity, 98% coverage: 7:385/385 of query aligns to 10:396/396 of 4ubtA
- active site: S98 (≠ C94), H352 (= H341), C382 (= C371), G384 (= G373)
- binding (2S)-2-[(8S,9S,10R,13S,14S,17R)-10,13-dimethyl-3-oxo-2,3,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]propanoic acid (non-preferred name): Q97 (= Q93), S98 (≠ C94), R141 (≠ G141), Q156 (= Q154), A383 (≠ E372), G384 (= G373)
- binding coenzyme a: Q156 (= Q154), R226 (= R213), T228 (≠ S216), L236 (= L224), A247 (= A236), S251 (= S240), I253 (≠ L242)
P45359 Acetyl-CoA acetyltransferase; Acetoacetyl-CoA thiolase; CaTHL; EC 2.3.1.9 from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM 19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W) (see paper)
42% identity, 99% coverage: 4:383/385 of query aligns to 2:390/392 of P45359
- V77 (≠ E83) mutation to Q: 3-fold increase in thiolase activity, prevents disulfide bond formation under oxidized condition and results in the loss of regulatory mechanism based on redox-switch modulation; when associated with Y-153 and K-286.
- C88 (= C94) modified: Disulfide link with 378, In inhibited form
- S96 (≠ H102) binding acetate
- N153 (= N153) mutation to Y: 3-fold increase in thiolase activity, prevents disulfide bond formation under oxidized condition and results in the loss of regulatory mechanism based on redox-switch modulation; when associated with Q-77 and K-286.
- GS 279:280 (≠ SV 272:273) binding acetate
- A286 (≠ I279) mutation to K: 3-fold increase in thiolase activity, prevents disulfide bond formation under oxidized condition and results in the loss of regulatory mechanism based on redox-switch modulation; when associated with Q-77 and Y-153.
- C378 (= C371) modified: Disulfide link with 88, In inhibited form
- A386 (= A379) binding acetate
4xl4A Crystal structure of thiolase from clostridium acetobutylicum in complex with coa (see paper)
41% identity, 99% coverage: 4:383/385 of query aligns to 2:390/392 of 4xl4A
- active site: C88 (= C94), H348 (= H341), S378 (≠ C371), G380 (= G373)
- binding coenzyme a: L148 (≠ Y148), H156 (vs. gap), R220 (= R213), L231 (= L224), A243 (= A236), S247 (= S240), F319 (= F312), H348 (= H341)
Q56WD9 3-ketoacyl-CoA thiolase 2, peroxisomal; Acetyl-CoA acyltransferase 2; Beta-ketothiolase 2; Peroxisomal 3-oxoacyl-CoA thiolase 2; Peroxisome defective protein 1; EC 2.3.1.16 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
40% identity, 98% coverage: 5:383/385 of query aligns to 51:437/462 of Q56WD9
- C138 (= C94) modified: Disulfide link with 192
- C192 (≠ K150) modified: Disulfide link with 138
P09110 3-ketoacyl-CoA thiolase, peroxisomal; Acetyl-CoA C-myristoyltransferase; Acetyl-CoA acyltransferase; Beta-ketothiolase; Peroxisomal 3-oxoacyl-CoA thiolase; EC 2.3.1.16; EC 2.3.1.155; EC 2.3.1.9 from Homo sapiens (Human) (see 3 papers)
39% identity, 99% coverage: 2:383/385 of query aligns to 34:420/424 of P09110
- V387 (≠ A351) to A: in dbSNP:rs2229528
Sites not aligning to the query:
- 1:26 PTS2-type peroxisomal targeting signal
- 5 mutation Q->D,K,L: Does not affect localization to peroxisomes.
- 6 V→D: Abolished localization to peroxisomes.; V→K: Does not affect localization to peroxisomes.
- 7 mutation V->D,K: Abolished localization to peroxisomes.
- 8 mutation L->D,K: Does not affect localization to peroxisomes.
- 9 mutation G->D,R,L: Does not affect localization to peroxisomes.
- 10 H→E: In S3E mutant; Abolished localization to peroxisomes.
5f38B X-ray crystal structure of a thiolase from escherichia coli at 1.8 a resolution (see paper)
40% identity, 99% coverage: 4:385/385 of query aligns to 2:391/391 of 5f38B
- active site: C88 (= C94), H347 (= H341), C377 (= C371), G379 (= G373)
- binding coenzyme a: C88 (= C94), L149 (vs. gap), K219 (vs. gap), F234 (= F228), A242 (= A236), S246 (= S240), A317 (= A311), F318 (= F312), H347 (= H341)
8oqoC Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-49 (see paper)
41% identity, 87% coverage: 48:383/385 of query aligns to 45:396/398 of 8oqoC
1ou6A Biosynthetic thiolase from zoogloea ramigera in complex with acetyl-o- pantetheine-11-pivalate
39% identity, 100% coverage: 2:385/385 of query aligns to 1:392/392 of 1ou6A