SitesBLAST
Comparing WP_058930225.1 NCBI__GCF_001484605.1:WP_058930225.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5y8iA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + (s)-3-hydroxyisobutyrate (s-hiba) (see paper)
54% identity, 98% coverage: 5:303/304 of query aligns to 1:292/292 of 5y8iA
5y8hA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + NAD+ (see paper)
54% identity, 98% coverage: 5:302/304 of query aligns to 1:291/291 of 5y8hA
- binding (2~{S})-2-methylpentanedioic acid: R144 (= R154), E148 (= E158), A151 (≠ G161), K153 (≠ R163)
- binding nicotinamide-adenine-dinucleotide: G7 (= G11), G9 (= G13), N10 (≠ H14), M11 (= M15), F29 (= F33), D30 (= D34), P31 (≠ V35), M63 (= M67), L64 (≠ F68), G120 (= G130), L239 (= L249), K242 (= K252)
5y8lB Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + NAD +(s)-3-hydroxyisobutyrate (s-hiba) (see paper)
55% identity, 97% coverage: 4:299/304 of query aligns to 1:289/290 of 5y8lB
- binding (2~{S})-2-methylpentanedioic acid: T129 (= T138), E149 (= E158), A152 (≠ G161), G153 (≠ K162), G153 (≠ K162), K154 (≠ R163)
- binding (2S)-2-methyl-3-oxidanyl-propanoic acid: S119 (= S128), G120 (= G129), W211 (= W220), F236 (= F245)
- binding nicotinamide-adenine-dinucleotide: G8 (= G11), G10 (= G13), N11 (≠ H14), M12 (= M15), F30 (= F33), D31 (= D34), P32 (≠ V35), M64 (= M67), L65 (≠ F68), T93 (= T102), G121 (= G130), K168 (= K177), L240 (= L249), K243 (= K252)
5y8kA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + l-serine (see paper)
55% identity, 97% coverage: 4:299/304 of query aligns to 1:289/290 of 5y8kA
P31937 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Homo sapiens (Human) (see paper)
43% identity, 98% coverage: 4:301/304 of query aligns to 39:333/336 of P31937
- 40:68 (vs. 5:33, 52% identical) binding NAD(+)
- LP 103:104 (≠ FP 68:69) binding NAD(+)
- N108 (≠ H73) binding NAD(+)
- T134 (= T102) binding NAD(+)
- K284 (= K252) binding NAD(+)
Sites not aligning to the query:
- 1:36 modified: transit peptide, Mitochondrion
2i9pB Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+
43% identity, 97% coverage: 7:301/304 of query aligns to 3:294/296 of 2i9pB
- binding nicotinamide-adenine-dinucleotide: G9 (= G13), N10 (≠ H14), M11 (= M15), Y29 (≠ F33), D30 (= D34), V31 (= V35), M63 (= M67), L64 (≠ F68), P65 (= P69), T95 (= T102), V120 (= V127), G122 (= G129), F238 (= F245), K245 (= K252)
P29266 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Rattus norvegicus (Rat) (see paper)
41% identity, 99% coverage: 1:301/304 of query aligns to 35:332/335 of P29266
- D68 (= D34) mutation to R: Decrease of activity with NAD, increase of activity with NADP.
- K208 (= K177) mutation K->A,H,N,R: Complete loss of activity.
- N212 (= N181) mutation to Q: Decrease in activity.
Q9I5I6 NAD-dependent L-serine dehydrogenase; L-serine 3-dehydrogenase (NAD(+)); EC 1.1.1.387 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
46% identity, 98% coverage: 7:303/304 of query aligns to 4:296/298 of Q9I5I6
- P66 (= P69) binding NAD(+)
- T96 (= T102) binding NAD(+); mutation to A: Almost abolished activity.
- S122 (= S128) mutation to A: Strongly reduced activity.
- K171 (= K177) active site
- N175 (= N181) mutation to A: Strongly reduced activity.
- W214 (= W220) mutation to A: Almost abolished activity.
- Y219 (≠ N225) mutation to A: Strongly reduced activity.
- K246 (= K252) binding NAD(+); mutation to A: Almost abolished activity.
- D247 (= D253) mutation to A: Almost abolished activity.
Sites not aligning to the query:
3q3cA Crystal structure of a serine dehydrogenase from pseudomonas aeruginosa pao1 in complex with NAD (see paper)
46% identity, 98% coverage: 7:303/304 of query aligns to 3:294/294 of 3q3cA
- binding nicotinamide-adenine-dinucleotide: G9 (= G13), H10 (= H14), M11 (= M15), F29 (= F33), D30 (= D34), L31 (≠ V35), M63 (= M67), L64 (≠ F68), P65 (= P69), T94 (= T102), V119 (= V127), G121 (= G129), F237 (= F245), K244 (= K252)
3obbA Crystal structure of a possible 3-hydroxyisobutyrate dehydrogenase from pseudomonas aeruginosa pao1 (see paper)
46% identity, 98% coverage: 7:303/304 of query aligns to 4:295/295 of 3obbA
5je8B The crystal structure of bacillus cereus 3-hydroxyisobutyrate dehydrogenase in complex with NAD (see paper)
31% identity, 97% coverage: 7:301/304 of query aligns to 6:292/294 of 5je8B
3w6zA Crystal structure of NADP bound l-serine 3-dehydrogenase (k170m) from hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
32% identity, 93% coverage: 4:285/304 of query aligns to 13:284/296 of 3w6zA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G11), L21 (= L12), G22 (= G13), I23 (≠ H14), M24 (= M15), N43 (≠ D34), R44 (≠ V35), T45 (≠ V36), K48 (≠ A39), V77 (≠ F68), S78 (vs. gap), D82 (≠ S70), Q85 (≠ H73), V133 (= V127), F244 (= F245), K245 (≠ A246), H248 (≠ L249), K251 (= K252)
3ws7A The 1.18 a resolution structure of l-serine 3-dehydrogenase complexed with NADP+ and sulfate ion from the hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
33% identity, 93% coverage: 4:285/304 of query aligns to 13:281/293 of 3ws7A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G11), L21 (= L12), G22 (= G13), I23 (≠ H14), M24 (= M15), N43 (≠ D34), R44 (≠ V35), T45 (≠ V36), K48 (≠ A39), M76 (= M67), V77 (≠ F68), S78 (vs. gap), D82 (≠ S70), Q85 (≠ H73), V133 (= V127), F241 (= F245), K242 (≠ A246), H245 (≠ L249), K248 (= K252)
- binding sulfate ion: T134 (≠ S128), G135 (= G129), K183 (= K177)
2cvzC Structure of hydroxyisobutyrate dehydrogenase from thermus thermophilus hb8 (see paper)
35% identity, 93% coverage: 7:290/304 of query aligns to 4:272/289 of 2cvzC
- active site: S117 (= S128), K165 (= K177), N168 (= N180), N169 (= N181)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), L9 (= L12), G10 (= G13), A11 (≠ H14), M12 (= M15), N30 (≠ F33), R31 (≠ D34), T32 (≠ V35), C62 (≠ M67), L63 (≠ F68), P64 (= P69), E68 (≠ H73), E71 (≠ R79), S91 (≠ T102), V116 (= V127), F227 (= F245), K234 (= K252)
1wp4A Structure of tt368 protein from thermus thermophilus hb8 (see paper)
35% identity, 93% coverage: 7:290/304 of query aligns to 3:271/288 of 1wp4A
- active site: S116 (= S128), K164 (= K177), N167 (= N180), N168 (= N181)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G11), L8 (= L12), G9 (= G13), A10 (≠ H14), M11 (= M15), N29 (≠ F33), R30 (≠ D34), T31 (≠ V35), K34 (≠ A38), C61 (≠ M67), L62 (≠ F68), P63 (= P69), E67 (≠ H73), S90 (≠ T102), V115 (= V127), T225 (≠ G244), F226 (= F245), K233 (= K252)
- binding sulfate ion: S116 (= S128), G117 (= G129), G118 (= G130), K164 (= K177)
P0A9V8 3-sulfolactaldehyde reductase; SLA reductase; 4-hydroxybutyrate dehydrogenase; Gamma-hydroxybutyrate dehydrogenase; GHBDH; Succinic semialdehyde reductase; SSA reductase; EC 1.1.1.373; EC 1.1.1.61 from Escherichia coli (strain K12)
32% identity, 96% coverage: 7:299/304 of query aligns to 4:287/298 of P0A9V8
- QM 11:12 (≠ HM 14:15) binding NAD(+)
- D31 (= D34) binding NAD(+)
- L65 (≠ F68) binding NAD(+)
- T96 (= T102) binding NAD(+)
- G122 (≠ S128) mutation to S: 25-fold decrease in catalytic efficiency with SLA as substrate. 5-fold decrease in catalytic efficiency with NADH as substrate.
- R123 (≠ G129) binding 2,3-dihydroxypropane-1-sulfonate; mutation to G: 130-fold decrease in catalytic efficiency with SLA as substrate. 3-fold decrease in catalytic efficiency with NADH as substrate.
- T124 (≠ G130) mutation to G: 230-fold decrease in catalytic efficiency with SLA as substrate. 12-fold decrease in catalytic efficiency with NADH as substrate.
- NNY-MS 174:178 (≠ NNLILG 180:185) binding 2,3-dihydroxypropane-1-sulfonate
- K240 (= K252) binding NAD(+)
6smyA Crystal structure of sla reductase yihu from e. Coli with nadh and product dhps
32% identity, 96% coverage: 7:299/304 of query aligns to 3:286/294 of 6smyA
6smzC Crystal structure of sla reductase yihu from e. Coli in complex with nadh
32% identity, 96% coverage: 7:299/304 of query aligns to 3:286/295 of 6smzC
- binding nicotinamide-adenine-dinucleotide: G9 (= G13), Q10 (≠ H14), M11 (= M15), F29 (= F33), D30 (= D34), V31 (= V35), M63 (= M67), L64 (≠ F68), V73 (≠ A77), S94 (= S101), T95 (= T102), R122 (≠ G129)
3pduA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter sulfurreducens in complex with NADP+ (see paper)
32% identity, 96% coverage: 5:295/304 of query aligns to 2:282/287 of 3pduA
- binding glycerol: R242 (= R255), E246 (≠ N259), E246 (≠ N259), R250 (≠ S263)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), G10 (= G13), I11 (≠ H14), M12 (= M15), N31 (≠ D34), R32 (≠ V35), N33 (≠ V36), M64 (= M67), L65 (≠ F68), A66 (≠ P69), A70 (≠ H73), T96 (= T102), V121 (= V127), G123 (= G129), T124 (≠ G130), K171 (= K177), S231 (= S235), F232 (= F245), P233 (≠ A246), H236 (≠ L249), K239 (= K252)
3pefA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter metallireducens in complex with NADP+ (see paper)
30% identity, 95% coverage: 9:296/304 of query aligns to 6:283/287 of 3pefA
- binding glycerol: D67 (vs. gap), G123 (= G129), K171 (= K177), N175 (= N181), M178 (≠ L184), L203 (≠ F209), G207 (vs. gap), N213 (≠ G217), A217 (= A221), F232 (= F245), H236 (≠ L249), K239 (= K252), R242 (= R255), R269 (≠ A282)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G13), I11 (≠ H14), M12 (= M15), N31 (≠ D34), R32 (≠ V35), S33 (≠ V36), K36 (≠ A39), M64 (= M67), L65 (≠ F68), A66 (vs. gap), A70 (≠ G71), E73 (≠ V74), T96 (= T102), V121 (= V127), G123 (= G129), S124 (≠ G130), A231 (≠ G244), F232 (= F245), H236 (≠ L249), K239 (= K252)
Query Sequence
>WP_058930225.1 NCBI__GCF_001484605.1:WP_058930225.1
MPEQTTVAFLGLGHMGGPMAVNLVRAGYTVAGFDVVPAALDAAQTHGVPTVASPLDAIAG
ADVVLTMFPSGQHVLDAYRGADGQPGLLAEAKPGTMFLDCSTINVDEAREASALAIAAGH
RAVDAPVSGGVVGAEAGTLTFMVGGETEDFEAVRPLLEVMGKRVVHCGGHGGGQAAKICN
NLILGVSMIAVSEAFVLGEKLGLTHQALFDVASNASGQCWALTTNCPVPGPVPTSPANRD
YQPGFAGALMAKDLRLALNALESTGVAARLGPLASEIYDTFAAEGGAGRDFSGIITDIRD
KSQH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory