SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_058930844.1 NCBI__GCF_001484605.1:WP_058930844.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5ux5A Structure of proline utilization a (puta) from corynebacterium freiburgense (see paper)
45% identity, 89% coverage: 121:1155/1165 of query aligns to 28:961/963 of 5ux5A

query
sites
5ux5A

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active site: C617 (= C763), K792 (= K941)
binding flavin-adenine dinucleotide: D138 (= D241), M139 (= M242), R202 (= R305), V204 (= V307), K205 (= K308), G206 (= G309), A207 (= A310), L209 (= L312), T228 (= T331), K229 (= K332), V232 (≠ S335), S257 (≠ G361), N259 (= N363), E282 (= E386), M283 (= M387), N328 (≠ G432)
binding nicotinamide-adenine-dinucleotide: S484 (≠ P630), W486 (= W632), K510 (= K656), A512 (= A658), E543 (= E689), G547 (= G693), G563 (= G709), A564 (≠ G710), T567 (= T713), F571 (= F717), F713 (= F862)

5ur2B Crystal structure of proline utilization a (puta) from bdellovibrio bacteriovorus inactivated by n-propargylglycine (see paper)
32% identity, 70% coverage: 139:948/1165 of query aligns to 104:937/959 of 5ur2B

query
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5ur2B

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active site: N618 (= N633), K641 (= K656), E722 (= E729), C756 (= C763), E851 (= E860), T931 (≠ A942)
binding N-propargylglycine-modified flavin adenine dinucleotide: K174 (= K188), D215 (= D241), M216 (= M242), Q249 (= Q274), V278 (= V307), K279 (= K308), G280 (= G309), A281 (= A310), W283 (≠ L312), Y300 (≠ W329), T301 (≠ H330), N302 (≠ T331), K303 (= K332), S306 (= S335), A329 (= A360), S330 (≠ G361), H331 (= H362), N332 (= N363), Q356 (≠ E386), M357 (= M387), L358 (= L388), Y379 (= Y409), E398 (= E431), E403 (≠ D436), W405 (≠ F438)

4nmdA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca reduced with dithionite (see paper)
30% identity, 73% coverage: 107:953/1165 of query aligns to 80:956/979 of 4nmdA

query
sites
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D

active site: N631 (= N633), K654 (= K656), E735 (= E729), C769 (= C763), E865 (= E860), A945 (= A942)
binding dihydroflavine-adenine dinucleotide: D226 (= D241), M227 (= M242), V256 (= V272), Q258 (= Q274), R285 (= R305), V287 (= V307), K288 (= K308), G289 (= G309), A290 (= A310), W292 (≠ L312), W309 (= W329), T310 (≠ H330), I311 (≠ T331), K312 (= K332), S315 (= S335), A338 (= A360), S339 (≠ G361), H340 (= H362), N341 (= N363), Q365 (≠ E386), V366 (≠ M387), L367 (= L388), Y388 (= Y409), F414 (= F438)

4nmaA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca in complex with l-tetrahydro-2-furoic acid (see paper)
30% identity, 73% coverage: 109:953/1165 of query aligns to 82:954/977 of 4nmaA

query
sites
4nmaA

L

M

P

P

Q

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D

active site: N629 (= N633), K652 (= K656), E733 (= E729), C767 (= C763), E863 (= E860), A943 (= A942)
binding flavin-adenine dinucleotide: D226 (= D241), M227 (= M242), Q258 (= Q274), R285 (= R305), V287 (= V307), K288 (= K308), G289 (= G309), A290 (= A310), Y291 (≠ N311), W292 (≠ L312), W309 (= W329), T310 (≠ H330), I311 (≠ T331), K312 (= K332), S315 (= S335), A338 (= A360), S339 (≠ G361), H340 (= H362), N341 (= N363), Q365 (≠ E386), L367 (= L388), E407 (= E431), S413 (≠ N437), F414 (= F438)
binding tetrahydrofuran-2-carboxylic acid: K185 (≠ A194), Y388 (= Y409), Y400 (= Y424), R403 (= R427), R404 (= R428)

4nmeA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine (see paper)
30% identity, 72% coverage: 121:953/1165 of query aligns to 92:949/972 of 4nmeA

query
sites
4nmeA

L

I

R

E

E

E

M

M

V

A

G

R

H

Q

L

F

I

I

V

V

D

G

A

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A

K

K

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L

A

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K

A

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I

L

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K

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L

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R

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K

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D

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G

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active site: N624 (= N633), K647 (= K656), E728 (= E729), C762 (= C763), E858 (= E860), A938 (= A942)
binding N-propargylglycine-modified flavin adenine dinucleotide: K183 (≠ A194), D224 (= D241), M225 (= M242), V254 (= V272), Q256 (= Q274), R283 (= R305), V285 (= V307), K286 (= K308), G287 (= G309), A288 (= A310), W290 (≠ L312), W307 (= W329), T308 (≠ H330), I309 (≠ T331), K310 (= K332), S313 (= S335), A336 (= A360), S337 (≠ G361), H338 (= H362), N339 (= N363), Q363 (≠ E386), L365 (= L388), Y383 (= Y409), E402 (= E431), F409 (= F438)

4nmfB Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine and complexed with menadione bisulfite (see paper)
30% identity, 73% coverage: 107:953/1165 of query aligns to 79:956/979 of 4nmfB

query
sites
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N

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|
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G

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|
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A

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G

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A

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G

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G

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T

T

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K

A
|
A

G

G

G

G

P

P

N

D

Y

Y

L

L

A

L

G

H

L

F

G

M

D

D

active site: N631 (= N633), K654 (= K656), E735 (= E729), C769 (= C763), E865 (= E860), A945 (= A942)
binding (2R)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: K184 (≠ A194), Y290 (≠ N311), Y387 (= Y409), Y399 (= Y424), R402 (= R427), R403 (= R428)
binding (2S)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: K184 (≠ A194), L366 (= L388), Y399 (= Y424), R402 (= R427)
binding N-propargylglycine-modified flavin adenine dinucleotide: K184 (≠ A194), D225 (= D241), M226 (= M242), V255 (= V272), Q257 (= Q274), R284 (= R305), V286 (= V307), K287 (= K308), G288 (= G309), A289 (= A310), W291 (≠ L312), W308 (= W329), T309 (≠ H330), I310 (≠ T331), K311 (= K332), S314 (= S335), A337 (= A360), S338 (≠ G361), H339 (= H362), N340 (= N363), Q364 (≠ E386), L366 (= L388), Y387 (= Y409), E406 (= E431), E411 (≠ D436), S412 (≠ N437), F413 (= F438)

4nmcA Crystal structure of oxidized proline utilization a (puta) from geobacter sulfurreducens pca complexed with zwittergent 3-12 (see paper)
30% identity, 74% coverage: 91:953/1165 of query aligns to 53:919/941 of 4nmcA

query
sites
4nmcA

L

M

P

P

W

A

Y

F

M

M

R

S

S

T

A

G

V

A

R

K

L

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G

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M

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K

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K

K

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D

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G

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|
D

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|
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|
R

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|
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K
|
K

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|
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A
|
A

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S

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|
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|
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|
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|
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|
C

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D

active site: N594 (= N633), K617 (= K656), E698 (= E729), C732 (= C763), E828 (= E860), A908 (= A942)
binding flavin-adenine dinucleotide: D215 (= D241), M216 (= M242), V245 (= V272), Q247 (= Q274), R274 (= R305), V276 (= V307), K277 (= K308), G278 (= G309), A279 (= A310), W281 (≠ L312), W298 (= W329), T299 (≠ H330), I300 (≠ T331), K301 (= K332), S304 (= S335), A327 (= A360), S328 (≠ G361), H329 (= H362), N330 (= N363), L356 (= L388), Y377 (= Y409)

4nmfA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine and complexed with menadione bisulfite (see paper)
30% identity, 73% coverage: 107:953/1165 of query aligns to 76:950/973 of 4nmfA

query
sites
4nmfA

P

P

V

A

L

F

P

M

Q

A

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V

V

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K

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D

active site: N625 (= N633), K648 (= K656), E729 (= E729), C763 (= C763), E859 (= E860), A939 (= A942)
binding (2R)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: K181 (≠ A194), Y287 (≠ N311), Y384 (= Y409), Y396 (= Y424), R399 (= R427), R400 (= R428)
binding N-propargylglycine-modified flavin adenine dinucleotide: K181 (≠ A194), D222 (= D241), M223 (= M242), V252 (= V272), Q254 (= Q274), R281 (= R305), V283 (= V307), K284 (= K308), G285 (= G309), A286 (= A310), W288 (≠ L312), W305 (= W329), T306 (≠ H330), I307 (≠ T331), K308 (= K332), S311 (= S335), A334 (= A360), S335 (≠ G361), H336 (= H362), N337 (= N363), Q361 (≠ E386), V362 (≠ M387), L363 (= L388), Y384 (= Y409), E403 (= E431), E408 (≠ D436), F410 (= F438)

7na0A Structure of geobacter sulfurreducens proline utilization a (puta) variant a206w (see paper)
30% identity, 72% coverage: 121:953/1165 of query aligns to 94:957/981 of 7na0A

query
sites
7na0A

L

I

R

E

E

E

M

M

V

A

G

R

H

Q

L

F

I

I

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D

D

binding flavin-adenine dinucleotide: D226 (= D241), M227 (= M242), V256 (= V272), Q258 (= Q274), R285 (= R305), V287 (= V307), K288 (= K308), G289 (= G309), A290 (= A310), Y291 (≠ N311), W292 (≠ L312), W309 (= W329), T310 (≠ H330), I311 (≠ T331), K312 (= K332), S315 (= S335), A338 (= A360), S339 (≠ G361), H340 (= H362), N341 (= N363), L367 (= L388), Y388 (= Y409), E407 (= E431), S413 (≠ N437), F414 (= F438)

3hazA Crystal structure of bifunctional proline utilization a (puta) protein (see paper)
31% identity, 71% coverage: 118:949/1165 of query aligns to 151:967/983 of 3hazA

query
sites
3hazA

R

R

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active site: N652 (= N633), K675 (= K656), E752 (= E729), C786 (= C763), E878 (= E860), A960 (= A942)
binding flavin-adenine dinucleotide: D272 (= D241), A273 (≠ M242), Q306 (= Q274), R333 (= R305), V335 (= V307), K336 (= K308), G337 (= G309), A338 (= A310), Y339 (≠ N311), W340 (≠ L312), F358 (≠ A327), T359 (= T328), R360 (≠ T331), K361 (= K332), T364 (≠ S335), A387 (= A360), T388 (≠ G361), H389 (= H362), N390 (= N363), Y435 (= Y409), S460 (≠ N437), F461 (= F438)
binding nicotinamide-adenine-dinucleotide: I648 (≠ T629), S649 (≠ P630), P650 (= P631), W651 (= W632), N652 (= N633), I657 (= I638), K675 (= K656), P676 (= P657), A677 (= A658), G708 (≠ R690), G711 (= G693), A712 (≠ Q694), T726 (= T708), G727 (= G709), S728 (≠ G710), V731 (≠ T713), I735 (≠ F720), E752 (= E729), T753 (= T730), C786 (= C763), E878 (= E860), F880 (= F862), F948 (= F928)

6bsnA Structure of proline utilization a (puta) with proline bound in remote sites (see paper)
31% identity, 71% coverage: 118:949/1165 of query aligns to 148:958/973 of 6bsnA

query
sites
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A
|
A

G

G

G

G

P

P

N

H

Y

Y

L

L

A
|
A

active site: N643 (= N633), E743 (= E729), A777 (≠ C763), A951 (= A942)
binding dihydroflavine-adenine dinucleotide: D269 (= D241), A270 (≠ M242), Q303 (= Q274), R330 (= R305), V332 (= V307), K333 (= K308), G334 (= G309), A335 (= A310), Y336 (≠ N311), W337 (≠ L312), F355 (≠ A327), T356 (= T328), R357 (≠ T331), K358 (= K332), T361 (≠ S335), A384 (= A360), T385 (≠ G361), H386 (= H362), N387 (= N363), Y432 (= Y409), S457 (≠ N437), F458 (= F438)
binding proline: M630 (vs. gap), W642 (= W632), F644 (= F634), G718 (= G709), R776 (≠ K762), S778 (= S764), F871 (= F862), I930 (≠ T919), G931 (= G920), A932 (= A921), F939 (= F928), A958 (= A949)

Sites not aligning to the query:

binding proline: 959, 961

2eiwA Crystal analysis of delta1-pyrroline-5-carboxylate dehydrogenase from thermus thermophilus with bound l-proline
33% identity, 34% coverage: 548:948/1165 of query aligns to 88:503/516 of 2eiwA

query
sites
2eiwA

K

K

A

T

W

W

G

K

A

D

L

W

T

P

G

Q

D

E

Q

D

R

R

A

S

E

R

I

L

L

L

H

L

R

K

A

A

G

A

D

A

V

L

L

M

E

R

A

R

R

R

R

K

A

R

D

E

L

L

L

E

E

A

V

T

M

L

A

V

S

Y

E

E

T

V

G

G

K

K

T

N

I

W

D

V

Q

E

G

A

D

S

P

A

E

D

V

V

S

A

E
|
E

A

A

V

I

D

D

F

F

A

I

H

E

Y

Y

Y

Y

A

A

E

R

S

A

A

A

R

L

K

R

L

Y

E

R

-

Y

-

P

-

A

-

V

-

E

-

V

-

V

-

P

-

Y

-

P

-

G

K

E

V

D

D

N

G

E

A

S

T

F

F

Y

V

V

P

P

A

L

K

G

L

A

T

G

V

V

V

V

T

I

P

A

P

P

W

W

N
|
N

F
|
F

P

P

V

V

A

A

I

I

P

F

A

T

G

G

S

M

T

I

L

V

A

G

A

P

L

V

A

A

A

V

G

G

S

N

A

T

V

V

V

I

I

A

K
|
K

P

P

A

A

K

E

Q

D

A

A

R

V

R

V

S

V

G

G

A

A

V

K

M

V

I

F

E

E

A

I

L

F

W

H

E

E

A

A

G

G

V

F

P

P

K

P

D

G

V

V

L

V

T

N

M

F

V

L

Q

P

L

G

G

V

E

G

R

E

E

E

L

V

G

G

Q

A

Q

Y

L

L

I

V

S

E

H

H

P

P

S

R

V

I

D

R

R

F

V

I

I

N

L

F

T

T

G

G

G

S

Y

L

E

E

T

V

A

G

-

L

E

K

L

I

F

Y

R

E

S

A

F

A

R

G

K

R

D

L

L

A

P

P

-

G

-

Q

-

T

-

W

-

F

-

K

-

R

L

A

L

Y

A

V

E
|
E

T

T

S

G

G

G

K

K

N

N

A

A

I

I

I

I

V

V

T

D

P

E

S

T

A

A

D

D

L

F

D

D

L

L

A

A

A

A

K

E

D

G

V

V

A

V

Y

V

S

S

A

A

F

Y

G

G

H

F

A

Q

G

G

Q

Q

K

K

C
|
C

S
|
S

A

A

A

A

S

S

L

R

V

L

I

I

L

L

V

T

G

Q

S

G

V

A

A

Y

K

E

S

P

K

-

R

-

F

V

H

L

N

E

Q

R

L

V

V

L

D

K

A

R

V

A

T

E

S

R

L

L

K

S

V

V

G

G

Y

-

P

P

Q

A

D

E

P

E

T

N

S

P

Q

D

M

L

G

G

P

P

I

V

I

V

E

S

P

A

A

E

D

Q

G

E

K

R

L

K

L

V

N

L

A

S

L

Y

T

I

T

E

L

I

G

G

E

K

G

N

E

E

T

G

W

Q

A

L

V

V

-

L

E

G

P

G

K

K

K

R

L

L

D

E

E

G

T

E

G

G

R

Y

L

F

W

I

S

A

P

P

G

T

V

V

R

F

Y

T

G

E

V

V

R

P

R

P

G

K

S

A

Y

R

F

I

H

A

L

Q

T

E

E
|
E

F

I

F

F

G

G

P

P

V

V

L

L

G

S

V

V

M

I

T

R

A

V

D

K

T

D

L

F

E

A

E

E

A

A

I

L

A

E

I

V

Q

A

N

N

Q

D

I

T

E

P

Y

Y

G

G

L

L

T

T

A

G

G

G

L

V

H

Y

S

S

L

R

N

K

P

R

D

E

E

H

L

L

G

E

T

W

W

A

L

R

D

R

S

E

V

F

Q

H

A

V

G

G

N

N

L

L

Y

Y

V

F

N

N

R

R

G

K

I

I

T

T

G
|
G

A
|
A

I

L

V

V

Q

G

R

V

Q

Q

P

P

F
|
F

G

G

G

G

W

F

K

K

K

L

S

S

A

-

V

-

G

G

A

T

G

N

T

A

K

K

A
x
T

G

G

G

A

P

L

N

D

Y

Y

L

L

active site: N184 (= N633), K207 (= K656), E288 (= E729), C322 (= C763), E417 (= E860), T497 (≠ A942)
binding proline: E137 (= E597), F185 (= F634), S323 (= S764), G477 (= G920), A478 (= A921), F485 (= F928)

6x9dA Structure of proline utilization a with trans-4-hydroxy-l-proline bound in the l-glutamate-gamma-semialdehyde dehydrogenase active site (see paper)
30% identity, 61% coverage: 238:951/1165 of query aligns to 288:1016/1218 of 6x9dA

query
sites
6x9dA

I

L

N

N

L

I

D
|
D

M
x
A

E

E

E

E

Y

A

K

D

D

R

L

L

D

E

M

L

T

S

I

L

A

D

V

L

F

L

T

E

R

V

I

L

L

C

D

L

K

D

P

G

E

D

F

L

K

S

N

G

L

W

E

N

A

G

-

M

G

G

I

F

V
|
V

L

V

Q
|
Q

A

A

Y

Y

-

G

-

K

-

R

L

C

P

P

D

F

A

V

L

L

S

D

A

F

M

I

I

I

R

D

L

L

Q

A

D

-

W

-

A

-

A

-

E

-

R

R

R

R

A

S

N

-

G

-

G

G

A

R

A

R

I

I

K

M

V

V

R
|
R

V

L

V
|
V

K
|
K

G
|
G

A
|
A

N
x
Y

L
x
W

P

D

M

A

E

E

Q

I

V

K

E

R

S

A

S

Q

L

L

H

D

-

G

-

L

-

A

D

D

W

F

P

P

L

V

A
x
F

T
|
T

W

-

H

-

T
x
R

K
|
K

Q

I

D

H

S
x
T

D

D

T

V

N

S

Y

Y

K

I

R

A

V

C

I

A

N

A

Y

K

S

L

L

L

H

A

P

A

D

T

R

D

I

V

N

-

N

-

V

V

R

F

I

P

G

Q

V

F

A
|
A

G
x
T

H
|
H

N
|
N

L

A

F

Q

D

T

I

L

A

A

F

A

A

I

W

Y

L

H

L

M

A

A

K

G

Q

K

R

D

G

F

V

H

E

V

S

G

G

K

I

Y

E

E

F

F

E
x
Q

M
x
C

L

L

L

H

G

G

M

M

A

G

Q

E

G

P

Q

L

A

Y

E

E

A

E

V

V

-

V

-

G

-

R

K

G

K

K

D

L

V

D

G

R

S

P

L

C

L

R

L

I

Y

Y

T

A

P

P

V

V

V

-

H

-

P

-

A

G

E

T

F

H

D

E

V

T

A

L

I

L

A

A

Y

Y

L

L

I

V

R

R

R

R

L

L

E

L

E
|
E

G

N

A

G

S

A

Q

N

D

S

N
x
S

F
|
F

M

-

S

-

A

-

V

V

F

H

E

R

L

I

S

N

E

D

N

P

Q

K

V

V

L

S

F

I

E

D

R

E

E

-

K

-

Q

-

R

-

F

L

L

I

A

A

S

D

L

P

D

V

T

E

L

V

D

V

D

R

E

A

V

M

P

P

P

V

A

V

N

G

R

A

Q

K

Q

H

N

D

R

R

S

I

L

A

P

L

P

P

Q

A

P

E

L

L

P

F

R

G

D

D

T

A

F

R

A

T

N

N

T

S

P

A

D

G

T

L

D

D

P

L

S

S

-

N

-

E

-

E

-

T

-

L

-

A

-

S

-

L

L

T

P

E

A

A

N

L

R

R

T

E

W

S

G

A

R

A

A

M

I

K

L

W

D

T

R

A

V

L

P

P

T

Q

S

L

T

A

L

T

G

G

N

P

A

A

A

A

V

G

D

E

A

T

A

R

T

T

I

V

S

L

D

N

A

P

-

G

-

D

-

H

-

R

D

D

T

V

L

V

N

G

T

S

V

V

I

T

A

E

T

T

A

S

V

E

E

E

K

D

G

A

K

R

A

R

W

A

G

V

A

R

L

L

T

A

G

A

-

D

-

A

-

A

-

P

-

D

-

W

-

A

-

A

-

V

-

P

-

P

D

S

Q

E

R

R

A

A

E

A

I

C

L

L

H

D

R

R

A

A

G

A

D

E

V

L

L

M

E

Q

A

A

R

R

R

M

A

P

D

T

L

L

L

L

E

G

V

L

M

I

A

I

S

R

E

E

T

A

G

G

K

K

T

S

I

A

D

L

Q

N

G

A

D

I

P

A

E

E

V

V

S

R

E
|
E

A

A

V

I

D

D

F

F

A

L

H

R

Y

Y

Y

Y

A

A

E

E

S

Q

A

T

R

R

K

-

L

-

E

-

K

R

V

T

D

L

G

G

A

P

T

G

F

H

V

G

P

P

A

L

K

G

L

P

T

I

V

V

V

C

T
x
I

P
x
S

P
|
P

W
|
W

N
|
N

F
|
F

P

P

V

L

A

A

I
|
I

P

F

A

T

G

G

S

Q

T

I

L

A

A

A

A

A

L

L

A

V

A

A

G

G

S

N

A

P

V

V

V

L

I

A

K
|
K

P

P

A
|
A

K
x
E

Q

E

A

T

R

P

R

L

S

I

G

A

A

A

V

E

M

G

I

V

E

R

A

I

L

L

W

R

E

E

A

A

G

G

V

I

P

P

K

A

D

S

V

A

L

L

T

Q

M

L

V

L

Q

P

L

-

G

G

E

D

R
x
G

E

R

L

V

G
|
G

Q
x
A

Q

A

L

L

I

V

S

A

H

A

P

A

S

E

V

T

D

A

R

G

V

V

I

M

L

F

T
|
T

G
|
G

G
x
S

Y

T

E

E

T
x
V

A

A

E

R

L

L

F

I

R

Q

S

A

F

Q

R

L

K

A

D

D

-

R

-

L

-

S

-

P

-

A

-

G

-

R

-

P

L

I

P

P

L

L

L

I

A

A

E
|
E

T
|
T

S

G

G

G

K

Q

N

N

A

A

I

M

I

I

V

V

T

D

P

S

S

S

A

A

D

L

L

A

D

E

L

Q

A

V

A

V

K

G

D

D

V

V

A

I

Y

T

S

S

A

A

F

F

G

D

H

S

A

A

G

G

Q

Q

K
x
R

C
|
C

S
|
S

A

A

A

L

S

R

L

V

V

L

I

C

L

L

V

Q

G

E

S

D

V

V

A

A

K

-

S

-

K

D

R

R

F

I

H

L

N

T

Q

M

L

L

V

K

D

G

A

A

V

L

T

H

S

E

L

L

K

H

V

I

G

G

Y

R

P

T

Q

D

D

R

P

L

T

S

S

V

Q

D

M

V

G

G

P

P

I

V

I

I

-

T

-

S

E

E

P

A

A

K

D

D

G

N

-

I

-

E

K

K

L

H

L

I

N

E

A

R

L

M

T

R

T

G

L

L

G

G

E

-

G

-

E

-

T

-

W

-

A

-

V

-

E

-

P

-

K

R

K

K

L

V

D

E

E

Q

T

I

G

G

R

L

L

A

W

S

S

E

P

T

G

G

V

V

R

-

Y

-

G

-

V

-

R

-

R

-

G

G

S

T

Y

F

F

V

H

P

L

P

T

T

-

I

-

I

-

E

-

L

-

E

-

K

-

L

-

S

-

D

-

L

-

Q

-

R

E
|
E

F

V

F
|
F

G

G

P

P

V

V

L

L

G

H

V

V

M

I

-

R

-

Y

T

R

A

R

D

D

T

D

L

L

E

D

E

R

A

L

I

V

A

D

I

D

Q

V

N

N

Q

A

I

T

E

G

Y

Y

G

G

L

L

T

T

A

F

G

G

L

L

H

H

S

T

L

R

N

L

P

D

D

E

E

T

L

I

G

A

T

H

W

V

L

T

D

S

S

R

V

I

Q

K

A

A

G

G

N

N

L

L

Y

Y

V

I

N

N

R

R

G

N

I

I

T

I

G
|
G

A
|
A

I

V

V

V

Q

G

R

V

Q

Q

P

P

F
|
F

G

G

G

G

W

-

K

-

K

R

S

G

A

L

V

S

G

G

A

T

G

G

T

P

K

K

A
|
A

G

G

G

G

P

P

N

L

Y

Y

L

L

A

G

G

R

L

L

active site: N692 (= N633), K715 (= K656), E795 (= E729), C829 (= C763), E925 (= E860), A1007 (= A942)
binding flavin-adenine dinucleotide: D291 (= D241), A292 (≠ M242), V323 (= V272), Q325 (= Q274), R352 (= R305), V354 (= V307), K355 (= K308), G356 (= G309), A357 (= A310), Y358 (≠ N311), W359 (≠ L312), F377 (≠ A327), T378 (= T328), R379 (≠ T331), K380 (= K332), T383 (≠ S335), A406 (= A360), T407 (≠ G361), H408 (= H362), N409 (= N363), Q432 (≠ E386), C433 (≠ M387), E477 (= E431), S483 (≠ N437), F484 (= F438)
binding 4-hydroxyproline: E659 (= E597), F693 (= F634), I697 (= I638), R828 (≠ K762), S830 (= S764), G987 (= G920), A988 (= A921), F995 (= F928)
binding nicotinamide-adenine-dinucleotide: I688 (≠ T629), S689 (≠ P630), P690 (= P631), W691 (= W632), N692 (= N633), I697 (= I638), K715 (= K656), A717 (= A658), E718 (≠ K659), G748 (≠ R690), G751 (= G693), A752 (≠ Q694), T766 (= T708), G767 (= G709), S768 (≠ G710), V771 (≠ T713), E795 (= E729), T796 (= T730), C829 (= C763), E925 (= E860), F927 (= F862), F995 (= F928)

5kf6A Structure of proline utilization a from sinorhizobium meliloti complexed with l-tetrahydrofuroic acid and NAD+ in space group p21 (see paper)
30% identity, 61% coverage: 238:951/1165 of query aligns to 279:1007/1207 of 5kf6A

query
sites
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active site: N683 (= N633), K706 (= K656), E786 (= E729), C820 (= C763), E916 (= E860), A998 (= A942)
binding flavin-adenine dinucleotide: D282 (= D241), A283 (≠ M242), V314 (= V272), Q316 (= Q274), R343 (= R305), V345 (= V307), K346 (= K308), G347 (= G309), A348 (= A310), Y349 (≠ N311), W350 (≠ L312), F368 (≠ A327), T369 (= T328), R370 (≠ T331), K371 (= K332), T374 (≠ S335), A397 (= A360), T398 (≠ G361), H399 (= H362), N400 (= N363), Q423 (≠ E386), C424 (≠ M387), L425 (= L388), E468 (= E431), S474 (≠ N437), F475 (= F438)
binding nicotinamide-adenine-dinucleotide: I679 (≠ T629), S680 (≠ P630), P681 (= P631), W682 (= W632), N683 (= N633), I688 (= I638), K706 (= K656), A708 (= A658), E709 (≠ K659), G739 (≠ R690), G742 (= G693), A743 (≠ Q694), F756 (≠ L707), T757 (= T708), G758 (= G709), S759 (≠ G710), V762 (≠ T713), I766 (≠ F717), E786 (= E729), T787 (= T730), C820 (= C763), E916 (= E860), F918 (= F862), F986 (= F928)
binding tetrahydrofuran-2-carboxylic acid: D282 (= D241), Y449 (= Y409), R464 (= R427), R465 (= R428)

Sites not aligning to the query:

binding tetrahydrofuran-2-carboxylic acid: 241

7mybA Structure of proline utilization a with tetrahydrothiophene-2- carboxylate bound in the proline dehydrogenase active site (see paper)
30% identity, 61% coverage: 238:951/1165 of query aligns to 287:1015/1217 of 7mybA

query
sites
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P

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G

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Y

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I

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I

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E

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F
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F

G

G

P

P

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L

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H

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M

I

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R

-

Y

T

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A

R

D

D

T

D

L

L

E

D

E

R

A

L

I

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A

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I

D

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V

N

N

Q

A

I

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E

G

Y

Y

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F

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G

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W

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I

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K

A

A

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G

N

N

L

L

Y

Y

V

I

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N

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N

I

I

T

I

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G

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A

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F
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F

G

G

G

G

W

-

K

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K

R

S

G

A

L

V

S

G

G

A

T

G

G

T

P

K

K

A

A

G

G

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L

Y

Y

L

L

A

G

G

R

L

L

binding flavin-adenine dinucleotide: D290 (= D241), A291 (≠ M242), V322 (= V272), Q324 (= Q274), R351 (= R305), V353 (= V307), K354 (= K308), G355 (= G309), A356 (= A310), Y357 (≠ N311), W358 (≠ L312), F376 (≠ A327), T377 (= T328), R378 (≠ T331), K379 (= K332), T382 (≠ S335), A405 (= A360), T406 (≠ G361), H407 (= H362), N408 (= N363), C432 (≠ M387), L433 (= L388), E476 (= E431), S482 (≠ N437), F483 (= F438)
binding nicotinamide-adenine-dinucleotide: I687 (≠ T629), S688 (≠ P630), P689 (= P631), W690 (= W632), N691 (= N633), I696 (= I638), K714 (= K656), E717 (≠ K659), G747 (≠ R690), G750 (= G693), T765 (= T708), G766 (= G709), S767 (≠ G710), V770 (≠ T713), I774 (≠ F717), E794 (= E729), T795 (= T730), C828 (= C763), E924 (= E860), F926 (= F862), F994 (= F928)
binding (2S)-thiolane-2-carboxylic acid: Y457 (= Y409), Y469 (= Y424), R472 (= R427), R473 (= R428)
binding (2R)-thiolane-2-carboxylic acid: D290 (= D241), Y457 (= Y409), Y469 (= Y424), R472 (= R427), R473 (= R428)

Sites not aligning to the query:

7myaA Structure of proline utilization a with the fad covalently-modified by 1,3-dithiolane (see paper)
30% identity, 61% coverage: 238:951/1165 of query aligns to 287:1015/1217 of 7myaA

query
sites
7myaA

I

L

N

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M
x
A

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A

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C
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A

A

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L

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V

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I

C

L

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P

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I

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A

A

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N

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N

Q

A

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Y

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F

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A

A

G

G

N

N

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L

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Y

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N

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N

I

I

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I

G

G

A

A

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F
|
F

G

G

G

G

W

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K

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K

R

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G

A

L

V

S

G

G

A

T

G

G

T

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K

K

A

A

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G

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N

L

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Y

L

L

A

G

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R

L

L

binding nicotinamide-adenine-dinucleotide: I687 (≠ T629), S688 (≠ P630), P689 (= P631), W690 (= W632), N691 (= N633), I696 (= I638), K714 (= K656), A716 (= A658), E717 (≠ K659), G747 (≠ R690), G750 (= G693), A751 (≠ Q694), T765 (= T708), G766 (= G709), S767 (≠ G710), V770 (≠ T713), E794 (= E729), T795 (= T730), C828 (= C763), E924 (= E860), F926 (= F862), F994 (= F928)
binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[5-(1,3-dithiolan-2-yl)-7,8-dimethyl-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: D290 (= D241), A291 (≠ M242), V322 (= V272), Q324 (= Q274), V353 (= V307), K354 (= K308), G355 (= G309), A356 (= A310), W358 (≠ L312), F376 (≠ A327), T377 (= T328), R378 (≠ T331), K379 (= K332), T382 (≠ S335), A405 (= A360), T406 (≠ G361), H407 (= H362), N408 (= N363), Q431 (≠ E386), C432 (≠ M387), L433 (= L388), Y457 (= Y409), E476 (= E431)

Sites not aligning to the query:

binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[5-(1,3-dithiolan-2-yl)-7,8-dimethyl-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: 1217

6x9bA Structure of proline utilization a with cis-4-hydroxy-d-proline bound in the l-glutamate-gamma-semialdehyde dehydrogenase active site (see paper)
30% identity, 61% coverage: 238:951/1165 of query aligns to 284:1012/1214 of 6x9bA

query
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active site: N688 (= N633), K711 (= K656), E791 (= E729), C825 (= C763), E921 (= E860), A1003 (= A942)
binding flavin-adenine dinucleotide: D287 (= D241), A288 (≠ M242), V319 (= V272), R348 (= R305), V350 (= V307), K351 (= K308), G352 (= G309), A353 (= A310), Y354 (≠ N311), W355 (≠ L312), F373 (≠ A327), T374 (= T328), R375 (≠ T331), K376 (= K332), T379 (≠ S335), A402 (= A360), T403 (≠ G361), H404 (= H362), N405 (= N363), Q428 (≠ E386), C429 (≠ M387), Y454 (= Y409), E473 (= E431), S479 (≠ N437), F480 (= F438)
binding nicotinamide-adenine-dinucleotide: I684 (≠ T629), S685 (≠ P630), P686 (= P631), W687 (= W632), N688 (= N633), I693 (= I638), K711 (= K656), A713 (= A658), E714 (≠ K659), G744 (≠ R690), G747 (= G693), A748 (≠ Q694), T762 (= T708), G763 (= G709), S764 (≠ G710), V767 (≠ T713), I771 (≠ F717), E791 (= E729), T792 (= T730), C825 (= C763), E921 (= E860), F923 (= F862)
binding (4R)-4-hydroxy-D-proline: E655 (= E597), F689 (= F634), S826 (= S764), G983 (= G920), A984 (= A921), F991 (= F928)

6x9aA Structure of proline utilization a with trans-4-hydroxy-d-proline bound in the l-glutamate-gamma-semialdehyde dehydrogenase active site (see paper)
30% identity, 61% coverage: 238:951/1165 of query aligns to 284:1012/1214 of 6x9aA

query
sites
6x9aA

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active site: N688 (= N633), K711 (= K656), E791 (= E729), C825 (= C763), E921 (= E860), A1003 (= A942)
binding flavin-adenine dinucleotide: D287 (= D241), A288 (≠ M242), V319 (= V272), R348 (= R305), V350 (= V307), K351 (= K308), G352 (= G309), A353 (= A310), Y354 (≠ N311), W355 (≠ L312), F373 (≠ A327), T374 (= T328), R375 (≠ T331), K376 (= K332), T379 (≠ S335), A402 (= A360), T403 (≠ G361), H404 (= H362), N405 (= N363), C429 (≠ M387), E473 (= E431), S479 (≠ N437), F480 (= F438)
binding (4S)-4-hydroxy-D-proline: W555 (≠ L512), T556 (≠ D513), E655 (= E597), F689 (= F634), R725 (≠ E670), S826 (= S764), G983 (= G920), A984 (= A921), F991 (= F928)

6x99A Structure of proline utilization a with d-proline bound in the l- glutamate-gamma-semialdehyde dehydrogenase active site (see paper)
30% identity, 61% coverage: 238:951/1165 of query aligns to 286:1014/1216 of 6x99A

query
sites
6x99A

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I

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A
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A

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active site: N690 (= N633), K713 (= K656), E793 (= E729), C827 (= C763), E923 (= E860), A1005 (= A942)
binding d-proline: W557 (≠ L512), T558 (≠ D513), E657 (= E597), F691 (= F634), R727 (≠ E670), R826 (≠ K762), S828 (= S764), G985 (= G920), A986 (= A921), F993 (= F928)
binding flavin-adenine dinucleotide: D289 (= D241), A290 (≠ M242), V321 (= V272), R350 (= R305), V352 (= V307), K353 (= K308), G354 (= G309), A355 (= A310), Y356 (≠ N311), W357 (≠ L312), F375 (≠ A327), T376 (= T328), R377 (≠ T331), K378 (= K332), T381 (≠ S335), A404 (= A360), T405 (≠ G361), H406 (= H362), N407 (= N363), Q430 (≠ E386), C431 (≠ M387), Y456 (= Y409), E475 (= E431), S481 (≠ N437), F482 (= F438)

7my9A Structure of proline utilization a with 1,3-dithiolane-2-carboxylate bound in the proline dehydrogenase active site (see paper)
30% identity, 61% coverage: 238:951/1165 of query aligns to 286:1014/1216 of 7my9A

query
sites
7my9A

I

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A

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binding flavin-adenine dinucleotide: D289 (= D241), A290 (≠ M242), V321 (= V272), Q323 (= Q274), R350 (= R305), V352 (= V307), K353 (= K308), G354 (= G309), A355 (= A310), Y356 (≠ N311), W357 (≠ L312), F375 (≠ A327), T376 (= T328), R377 (≠ T331), K378 (= K332), T381 (≠ S335), A404 (= A360), T405 (≠ G361), H406 (= H362), N407 (= N363), C431 (≠ M387), L432 (= L388), E475 (= E431), S481 (≠ N437), F482 (= F438)
binding nicotinamide-adenine-dinucleotide: I686 (≠ T629), S687 (≠ P630), P688 (= P631), W689 (= W632), N690 (= N633), I695 (= I638), K713 (= K656), A715 (= A658), E716 (≠ K659), G746 (≠ R690), G749 (= G693), A750 (≠ Q694), T764 (= T708), G765 (= G709), S766 (≠ G710), V769 (≠ T713), E793 (= E729), T794 (= T730), C827 (= C763), E923 (= E860), F925 (= F862), F993 (= F928)
binding 1,3-dithiolane-2-carboxylic acid: Y456 (= Y409), Y468 (= Y424), R471 (= R427), R472 (= R428)

Query Sequence

>WP_058930844.1 NCBI__GCF_001484605.1:WP_058930844.1
MTHTATEAAGQTDVPQARALAADTITLVRRWLTEASKVPVDASAEQLAGVLKDPNGLDFT
VGFVDGVVRPEDLHVAARNLAALAPKVPAFLPWYMRSAVRLGGTMAPVLPQVVIPIARRV
LREMVGHLIVDATDAKLGPAIAKIRKDGIKLNVNLLGEAVLGEHEASRRLAGTHTLLARP
DVDYVSIKVSSTVAPHSAWAFDEAVEHVVEKLTPLFTKAASFAGAASGAGAATNAKFINL
DMEEYKDLDMTIAVFTRILDKPEFKNLEAGIVLQAYLPDALSAMIRLQDWAAERRANGGA
AIKVRVVKGANLPMEQVESSLHDWPLATWHTKQDSDTNYKRVINYSLHPDRINNVRIGVA
GHNLFDIAFAWLLAKQRGVESGIEFEMLLGMAQGQAEAVKKDVGSLLLYTPVVHPAEFDV
AIAYLIRRLEEGASQDNFMSAVFELSENQVLFEREKQRFLASLDTLDDEVPPANRQQNRS
LPPQPLPRDTFANTPDTDPSLPANRTWGRAILDRVPTSTLGNAAVDAATISDADTLNTVI
ATAVEKGKAWGALTGDQRAEILHRAGDVLEARRADLLEVMASETGKTIDQGDPEVSEAVD
FAHYYAESARKLEKVDGATFVPAKLTVVTPPWNFPVAIPAGSTLAALAAGSAVVIKPAKQ
ARRSGAVMIEALWEAGVPKDVLTMVQLGERELGQQLISHPSVDRVILTGGYETAELFRSF
RKDLPLLAETSGKNAIIVTPSADLDLAAKDVAYSAFGHAGQKCSAASLVILVGSVAKSKR
FHNQLVDAVTSLKVGYPQDPTSQMGPIIEPADGKLLNALTTLGEGETWAVEPKKLDETGR
LWSPGVRYGVRRGSYFHLTEFFGPVLGVMTADTLEEAIAIQNQIEYGLTAGLHSLNPDEL
GTWLDSVQAGNLYVNRGITGAIVQRQPFGGWKKSAVGAGTKAGGPNYLAGLGDWVSADAA
VTLAVTNAGVGRIQNAAAGELQPAEIESLQRSLGSDALAWAHEFGTAKDVSGLSAERNIF
RYRALPVTVRLSEGAPLAHLARTVAAGVLAGSALTVSTAVELPAQLRAVLTGLDITVTVE
SDAGWLASAARLAAAGRLSGARIRLIGGDATALAEATDGRPDLAIYAHAVTEAGRVELLP
FLHEQAISITAHRFGTPNHLSDELI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory