SitesBLAST
Comparing WP_058931418.1 NCBI__GCF_001484605.1:WP_058931418.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P77455 Bifunctional protein PaaZ; EC 3.3.2.12; EC 1.2.1.91 from Escherichia coli (strain K12) (see paper)
48% identity, 98% coverage: 12:700/700 of query aligns to 1:674/681 of P77455
- E256 (= E271) mutation to Q: Catalyzes the formation of 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde instead of 3-oxo-5,6-dehydrosuberyl-CoA.
6jqoA Structure of paaz, a bifunctional enzyme in complex with NADP+ and ccoa (see paper)
48% identity, 98% coverage: 13:700/700 of query aligns to 1:673/678 of 6jqoA
- active site: N157 (= N173), E255 (= E271), C294 (= C310), L483 (= L510)
- binding crotonyl coenzyme a: V97 (≠ I113), F107 (= F123), S111 (= S127), F158 (= F174), W161 (= W177), R638 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: N154 (= N170), F156 (= F172), N157 (= N173), T183 (= T199), T230 (= T246), G231 (= G247), S232 (= S248), T235 (= T251), A256 (≠ T272), D257 (= D273), C294 (= C310)
6jqnA Structure of paaz, a bifunctional enzyme in complex with NADP+ and ocoa (see paper)
48% identity, 98% coverage: 13:700/700 of query aligns to 1:673/678 of 6jqnA
- active site: N157 (= N173), E255 (= E271), C294 (= C310), L483 (= L510)
- binding octanoyl-coenzyme a: F562 (= F593), H565 (= H596), F576 (= F608), G583 (= G615), V595 (= V627), A604 (= A636), N605 (= N637), Y606 (= Y638), F613 (= F645), I614 (= I646)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R19 (≠ A34), I153 (= I169), N154 (= N170), A155 (= A171), F156 (= F172), K180 (= K196), A182 (= A198), T183 (= T199), T230 (= T246), G231 (= G247), S232 (= S248), T235 (= T251), L239 (= L255), E255 (= E271), A256 (≠ T272), D257 (= D273), C294 (= C310), F396 (= F423), H471 (= H498)
6jqmA Structure of paaz with NADPH (see paper)
48% identity, 98% coverage: 13:700/700 of query aligns to 1:673/678 of 6jqmA
- active site: N157 (= N173), E255 (= E271), C294 (= C310), L483 (= L510)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R19 (≠ A34), I153 (= I169), N154 (= N170), A155 (= A171), F156 (= F172), N157 (= N173), K180 (= K196), A182 (= A198), T183 (= T199), G231 (= G247), S232 (= S248), T235 (= T251), A256 (≠ T272), D257 (= D273), C294 (= C310), E394 (= E421), F396 (= F423)
2y53A Crystal structure of e257q mutant of the box pathway encoded aldh from burkholderia xenovorans lb400 (see paper)
43% identity, 66% coverage: 35:498/700 of query aligns to 22:483/529 of 2y53A
- active site: N160 (= N173), K183 (= K196), Q258 (≠ E271), C297 (= C310), E401 (= E421)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I156 (= I169), N157 (= N170), F159 (= F172), N160 (= N173), K183 (= K196), A185 (= A198), T186 (= T199), S217 (≠ V230), F232 (= F245), G234 (= G247), S235 (= S248), A236 (= A249), T238 (= T251), A259 (≠ T272), D260 (= D273), C297 (= C310), F403 (= F423)
Sites not aligning to the query:
2vroA Crystal structure of aldehyde dehydrogenase from burkholderia xenovorans lb400 (see paper)
43% identity, 66% coverage: 35:498/700 of query aligns to 22:484/521 of 2vroA
- active site: N160 (= N173), K183 (= K196), E258 (= E271), C297 (= C310), E401 (= E421)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I156 (= I169), K183 (= K196), S217 (≠ V230), S235 (= S248), T238 (= T251), L242 (= L255), F403 (= F423)
Sites not aligning to the query:
3ty7B Crystal structure of aldehyde dehydrogenase family protein from staphylococcus aureus
27% identity, 44% coverage: 164:470/700 of query aligns to 139:429/454 of 3ty7B
Sites not aligning to the query:
3b4wA Crystal structure of mycobacterium tuberculosis aldehyde dehydrogenase complexed with NAD+
28% identity, 54% coverage: 77:457/700 of query aligns to 67:420/483 of 3b4wA
- active site: N154 (= N173), K177 (= K196), E251 (= E271), C285 (= C310), E384 (= E421)
- binding nicotinamide-adenine-dinucleotide: I150 (= I169), V151 (≠ N170), W153 (≠ F172), N154 (= N173), K177 (= K196), I210 (≠ V230), G213 (≠ L233), T228 (≠ S248), G229 (≠ A249), S230 (= S250), V233 (≠ L253), E236 (≠ K256), E251 (= E271), L252 (≠ T272), C285 (= C310), E384 (= E421), F386 (= F423)
Sites not aligning to the query:
5ac0A Ovis aries aldehyde dehydrogenase 1a1 in complex with a duocarmycin analog (see paper)
26% identity, 71% coverage: 18:511/700 of query aligns to 17:472/494 of 5ac0A