SitesBLAST
Comparing WP_058931617.1 NCBI__GCF_001484605.1:WP_058931617.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4u6sA Ctbp1 in complex with substrate phenylpyruvate (see paper)
36% identity, 91% coverage: 24:320/325 of query aligns to 20:324/328 of 4u6sA
- active site: S99 (≠ G103), R241 (= R238), D265 (= D262), E270 (= E267), H290 (= H286)
- binding nicotinamide-adenine-dinucleotide: T103 (≠ V107), G156 (= G154), G158 (= G156), R159 (≠ L157), V160 (≠ I158), Y178 (≠ S176), D179 (= D177), P180 (= P178), Y181 (≠ L179), H211 (= H208), C212 (≠ V209), G213 (≠ P210), N218 (≠ T215), T239 (= T236), A240 (≠ C237), R241 (= R238), H290 (= H286), W293 (= W289)
- binding 3-phenylpyruvic acid: Y51 (≠ V55), H52 (≠ Q56), I73 (≠ Y77), G74 (= G78), S75 (≠ V79), G76 (= G80), R241 (= R238), W293 (= W289), M302 (≠ L298)
4u6qA Ctbp1 bound to inhibitor 2-(hydroxyimino)-3-phenylpropanoic acid (see paper)
36% identity, 91% coverage: 24:320/325 of query aligns to 20:324/328 of 4u6qA
- active site: S99 (≠ G103), R241 (= R238), D265 (= D262), E270 (= E267), H290 (= H286)
- binding (2E)-2-(hydroxyimino)-3-phenylpropanoic acid: Y51 (≠ V55), I73 (≠ Y77), G74 (= G78), S75 (≠ V79), G76 (= G80), R241 (= R238), H290 (= H286), W293 (= W289), M302 (≠ L298)
- binding 1,4-dihydronicotinamide adenine dinucleotide: S75 (≠ V79), T103 (≠ V107), G156 (= G154), R159 (≠ L157), V160 (≠ I158), Y178 (≠ S176), D179 (= D177), P180 (= P178), Y181 (≠ L179), H211 (= H208), C212 (≠ V209), G213 (≠ P210), N218 (≠ T215), T239 (= T236), A240 (≠ C237), R241 (= R238), H290 (= H286), W293 (= W289)
1hl3A Ctbp/bars in ternary complex with NAD(h) and pidlskk peptide (see paper)
36% identity, 91% coverage: 24:320/325 of query aligns to 20:324/331 of 1hl3A
- active site: S99 (≠ G103), R241 (= R238), D265 (= D262), E270 (= E267), H290 (= H286)
- binding nicotinamide-adenine-dinucleotide: T103 (≠ V107), G158 (= G156), R159 (≠ L157), V160 (≠ I158), D179 (= D177), Y181 (≠ L179), H211 (= H208), C212 (≠ V209), G213 (≠ P210), N218 (≠ T215), T239 (= T236), A240 (≠ C237), R241 (= R238), D265 (= D262), H290 (= H286)
- binding : V26 (= V30), A27 (≠ E31), F28 (≠ L32), C29 (≠ V33), E36 (= E40), H38 (vs. gap), E39 (= E41)
Sites not aligning to the query:
1hkuA Ctbp/bars: a dual-function protein involved in transcription corepression and golgi membrane fission (see paper)
36% identity, 91% coverage: 24:320/325 of query aligns to 20:324/331 of 1hkuA
- active site: S99 (≠ G103), R241 (= R238), D265 (= D262), E270 (= E267), H290 (= H286)
- binding nicotinamide-adenine-dinucleotide: S75 (≠ V79), T103 (≠ V107), G156 (= G154), G158 (= G156), R159 (≠ L157), V160 (≠ I158), Y178 (≠ S176), D179 (= D177), P180 (= P178), Y181 (≠ L179), C212 (≠ V209), N218 (≠ T215), T239 (= T236), A240 (≠ C237), R241 (= R238), H290 (= H286), W293 (= W289)
Sites not aligning to the query:
4lceA Ctbp1 in complex with substrate mtob (see paper)
36% identity, 91% coverage: 24:320/325 of query aligns to 19:323/327 of 4lceA
- active site: S98 (≠ G103), R240 (= R238), D264 (= D262), E269 (= E267), H289 (= H286)
- binding 4-(methylsulfanyl)-2-oxobutanoic acid: R71 (= R76), G73 (= G78), S74 (≠ V79), G75 (= G80), R240 (= R238), H289 (= H286), W292 (= W289)
- binding nicotinamide-adenine-dinucleotide: S74 (≠ V79), T102 (≠ V107), G155 (= G154), G157 (= G156), R158 (≠ L157), V159 (≠ I158), Y177 (≠ S176), D178 (= D177), P179 (= P178), Y180 (≠ L179), H210 (= H208), C211 (≠ V209), N214 (= N212), N217 (≠ T215), T238 (= T236), A239 (≠ C237), R240 (= R238), W292 (= W289)
6cdfA Human ctbp1 (28-378) (see paper)
36% identity, 91% coverage: 24:320/325 of query aligns to 21:325/333 of 6cdfA
- binding 1,4-dihydronicotinamide adenine dinucleotide: T104 (≠ V107), G157 (= G154), R160 (≠ L157), V161 (≠ I158), Y179 (≠ S176), D180 (= D177), P181 (= P178), Y182 (≠ L179), H212 (= H208), C213 (≠ V209), N219 (≠ T215), T240 (= T236), A241 (≠ C237), R242 (= R238), H291 (= H286), W294 (= W289)
6v89A Human ctbp1 (28-375) in complex with amp (see paper)
36% identity, 91% coverage: 24:320/325 of query aligns to 20:324/332 of 6v89A
Q9Z2F5 C-terminal-binding protein 1; CtBP1; 50 kDa BFA-dependent ADP-ribosylation substrate; BARS-50; C-terminal-binding protein 3; CtBP3; EC 1.1.1.- from Rattus norvegicus (Rat) (see 3 papers)
36% identity, 91% coverage: 24:320/325 of query aligns to 34:338/430 of Q9Z2F5
- A41 (≠ E31) mutation to E: Strongly reduces interaction with E1A.
- V55 (= V43) mutation to R: Strongly reduces interaction with E1A.
- S89 (≠ V79) binding NAD(+)
- IGLGRV 169:174 (≠ VGLGLI 153:158) binding NAD(+)
- G172 (= G156) mutation to E: Loss dimerization and of NAD binding.
- D193 (= D177) binding NAD(+)
- 226:232 (vs. 209:215, 43% identical) binding NAD(+)
- TAR 253:255 (≠ TCR 236:238) binding NAD(+)
- D279 (= D262) binding NAD(+)
Q13363 C-terminal-binding protein 1; CtBP1; EC 1.1.1.- from Homo sapiens (Human) (see 4 papers)
36% identity, 91% coverage: 24:320/325 of query aligns to 45:349/440 of Q13363
- A52 (≠ E31) mutation to E: Loss of interaction with SIMC1. No effect on its proteolytic processing mediated by CAPN3.
- V66 (= V43) mutation to R: Loss of interaction with SIMC1. Reduced proteolytic processing mediated by CAPN3.
- C134 (≠ A113) mutation to A: Strongly reduces E1A binding; when associated with A-138; A-141 and A-150.
- N138 (≠ S117) mutation to A: Strongly reduces E1A binding; when associated with A-134; A-141 and A-150.
- R141 (= R120) mutation to A: Strongly reduces E1A binding; when associated with A-134; A-138 and A-150.
- RR 141:142 (≠ RG 120:121) mutation to AA: Strongly reduces E1A binding; when associated with A-163 and A-171.
- L150 (≠ M129) mutation to A: Strongly reduces E1A binding; when associated with A-134; A-138 and A-141.
- R163 (≠ Q140) mutation to A: Strongly reduces E1A binding; when associated with A-141; A-142 and A-171.
- R171 (= R148) mutation to A: Strongly reduces E1A binding; when associated with A-141; A-142 and A-163.
- G181 (= G154) mutation to V: Strongly reduces E1A binding; when associated with V-183 and A-204.
- G183 (= G156) mutation to A: Reduced proteolytic processing mediated by CAPN3; when associated with A-186.; mutation to V: Strongly reduces E1A binding; when associated with V-181 and A-204.
- G186 (= G159) mutation to A: Reduced proteolytic processing mediated by CAPN3; when associated with A-183.
- D204 (= D177) mutation to A: Strongly reduces E1A binding; when associated with V-181 and V-183.; mutation to L: Reduced proteolytic processing mediated by CAPN3.
- R266 (= R238) mutation to A: Strongly reduces E1A binding; when associated with A-290; A-295 and A-315.
- D290 (= D262) mutation to A: Strongly reduces E1A binding; when associated with A-266; A-295 and A-315.
- E295 (= E267) mutation to A: Strongly reduces E1A binding; when associated with A-266; A-290 and A-315.
- H315 (= H286) mutation to A: Strongly reduces E1A binding; when associated with A-266; A-290 and A-295.
Sites not aligning to the query:
- 375:376 Cleavage; by CAPN1
- 387:388 Cleavage; by CAPN1
- 409:410 Cleavage; by CAPN1 and CAPN3
- 422 modified: Phosphoserine; by HIPK2; S→A: Abolishes phosphorylation by HIPK2 and prevents UV-induced clearance.
- 428 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
8atiA Human ctbp2(31-364) in complex with rai2 peptide(315-322)
36% identity, 91% coverage: 24:320/325 of query aligns to 19:323/330 of 8atiA
- binding nicotinamide-adenine-dinucleotide: S74 (≠ V79), T102 (≠ V107), G155 (= G154), G157 (= G156), R158 (≠ L157), T159 (≠ I158), D178 (= D177), P179 (= P178), Y180 (≠ L179), H210 (= H208), C211 (≠ V209), N212 (≠ P210), A238 (≠ T236), R240 (= R238), H289 (= H286), A291 (= A288), W292 (= W289)
- binding : V25 (= V30), A26 (≠ E31), F27 (≠ L32), C28 (≠ V33), E35 (= E40), H37 (vs. gap)
Sites not aligning to the query:
4lcjA Ctbp2 in complex with substrate mtob (see paper)
36% identity, 91% coverage: 24:320/325 of query aligns to 19:323/330 of 4lcjA
- active site: A98 (≠ G103), R240 (= R238), D264 (= D262), E269 (= E267), H289 (= H286)
- binding 4-(methylsulfanyl)-2-oxobutanoic acid: Y50 (≠ V55), H51 (≠ Q56), I72 (≠ Y77), G73 (= G78), S74 (≠ V79), G75 (= G80), R240 (= R238), H289 (= H286), W292 (= W289)
- binding nicotinamide-adenine-dinucleotide: S74 (≠ V79), T102 (≠ V107), I154 (≠ V153), G155 (= G154), G157 (= G156), R158 (≠ L157), T159 (≠ I158), D178 (= D177), Y180 (≠ L179), H210 (= H208), C211 (≠ V209), N212 (≠ P210), N214 (= N212), N217 (≠ T215), A238 (≠ T236), A239 (≠ C237), R240 (= R238), H289 (= H286), W292 (= W289)
P56545 C-terminal-binding protein 2; CtBP2 from Homo sapiens (Human)
36% identity, 93% coverage: 24:325/325 of query aligns to 51:363/445 of P56545
- IGFGRT 186:191 (≠ VGLGLI 153:158) binding NAD(+)
- D210 (= D177) binding NAD(+)
- 243:249 (vs. 209:215, 43% identical) binding NAD(+)
- AAR 270:272 (≠ TCR 236:238) binding NAD(+)
- D296 (= D262) binding NAD(+)
- HTAW 321:324 (≠ HAAW 286:289) binding NAD(+)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
39% identity, 82% coverage: 22:286/325 of query aligns to 15:278/304 of 1wwkA
- active site: S96 (≠ G103), R230 (= R238), D254 (= D262), E259 (= E267), H278 (= H286)
- binding nicotinamide-adenine-dinucleotide: V100 (= V107), G146 (= G154), F147 (≠ L155), G148 (= G156), R149 (≠ L157), I150 (= I158), Y168 (≠ S176), D169 (= D177), P170 (= P178), V201 (= V209), P202 (= P210), T207 (= T215), T228 (= T236), S229 (≠ C237), D254 (= D262), H278 (= H286)
Sites not aligning to the query:
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
37% identity, 87% coverage: 41:324/325 of query aligns to 37:326/334 of 5aovA
- active site: L100 (≠ G103), R241 (= R238), D265 (= D262), E270 (= E267), H288 (= H286)
- binding glyoxylic acid: M52 (≠ Q56), L53 (≠ Y57), L53 (≠ Y57), Y74 (= Y77), A75 (≠ G78), V76 (= V79), G77 (= G80), R241 (= R238), H288 (= H286)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V79), T104 (≠ V107), F158 (≠ L155), G159 (= G156), R160 (≠ L157), I161 (= I158), S180 (≠ D177), R181 (≠ P178), A211 (≠ H208), V212 (= V209), P213 (= P210), T218 (= T215), I239 (≠ T236), A240 (≠ C237), R241 (= R238), H288 (= H286), G290 (vs. gap)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
38% identity, 87% coverage: 41:324/325 of query aligns to 36:325/332 of 6biiA
- active site: L99 (≠ G103), R240 (= R238), D264 (= D262), E269 (= E267), H287 (= H286)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V79), T103 (≠ V107), G156 (= G154), F157 (≠ L155), G158 (= G156), R159 (≠ L157), I160 (= I158), A179 (≠ V173), R180 (≠ I174), S181 (≠ G175), K183 (≠ D177), V211 (= V209), P212 (= P210), E216 (≠ H214), T217 (= T215), V238 (≠ T236), A239 (≠ C237), R240 (= R238), D264 (= D262), H287 (= H286), G289 (vs. gap)
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
30% identity, 81% coverage: 54:316/325 of query aligns to 48:324/334 of 3kb6B
- active site: S97 (≠ G103), R231 (= R238), D255 (= D262), E260 (= E267), H294 (= H286)
- binding lactic acid: F49 (≠ V55), S72 (≠ G78), V73 (= V79), G74 (= G80), Y96 (= Y102), R231 (= R238), H294 (= H286)
- binding nicotinamide-adenine-dinucleotide: V73 (= V79), Y96 (= Y102), V101 (= V107), G150 (= G156), R151 (≠ L157), I152 (= I158), D171 (≠ T172), V172 (= V173), P203 (= P210), T229 (= T236), A230 (≠ C237), R231 (= R238), H294 (= H286), A296 (= A288), Y297 (≠ W289)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
37% identity, 87% coverage: 41:322/325 of query aligns to 34:314/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
37% identity, 87% coverage: 41:322/325 of query aligns to 33:313/526 of 3dc2A
Sites not aligning to the query:
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
31% identity, 91% coverage: 29:324/325 of query aligns to 27:321/533 of O43175
- T78 (≠ V79) binding NAD(+)
- R135 (≠ L136) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ LI 157:158) binding NAD(+)
- D175 (= D177) binding NAD(+)
- T207 (≠ V209) binding NAD(+)
- CAR 234:236 (≠ TCR 236:238) binding NAD(+)
- D260 (= D262) binding NAD(+)
- V261 (= V263) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HAAW 286:289) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
7dkmA Phgdh covalently linked to oridonin (see paper)
33% identity, 82% coverage: 29:296/325 of query aligns to 23:289/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V79), A102 (≠ V107), G148 (= G154), R151 (≠ L157), I152 (= I158), Y170 (≠ S176), D171 (= D177), P172 (= P178), I173 (≠ L179), H202 (= H208), T203 (≠ V209), P204 (= P210), T209 (= T215), C230 (≠ T236), A231 (≠ C237), R232 (= R238), H279 (= H286), G281 (≠ A288)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 293
Query Sequence
>WP_058931617.1 NCBI__GCF_001484605.1:WP_058931617.1
MPATPTRIVITDCDHDSIAIEQAVADAAGVELVLAQCRTEEDVIAAAAGADAIVVQYAPI
TDRVLAALPQLKAIGRYGVGVDTLDVEAATARGVAVCNVPDYGTEDVSDHAIALAVSLAR
GITRLDRGMRRGEHSLLPVQPLHRMSTRVFGVVGLGLIGAATARKAKGLGYTVIGSDPLA
EIGTTTADGVAVVGFEEVISRADVVSLHVPLNSHTHHLINAGVLAQMKRGAVLVNTCRGG
VVDTAAVADALASGQLYGAGLDVFEEEPLALTSPLIGCENAVLTPHAAWYSEESYAELKR
RTIENVLEVCAGRMPRNVMNAGVLV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory