SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_058931921.1 NCBI__GCF_001484605.1:WP_058931921.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
31% identity, 70% coverage: 33:121/128 of query aligns to 7:95/386 of P0A9J8

query
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P0A9J8

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R11 (= R37) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R54) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K65) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E78) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (≠ H114) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

1ecmB Atomic structure of the buried catalytic pocket of escherichia coli chorismate mutase
31% identity, 70% coverage: 33:121/128 of query aligns to 2:90/95 of 1ecmB

query
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1ecmB

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binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R23 (= R54), V30 (= V61), K34 (= K65), R42 (≠ G73), D43 (= D74), R46 (= R77), E47 (= E78), I76 (= I107), S79 (≠ V110), Q83 (≠ H114)

Query Sequence

>WP_058931921.1 NCBI__GCF_001484605.1:WP_058931921.1
MTQQNHDVPDTDTYDAAASSLAGHVDNSVMAELLSIRSSIDNIDATLVYLLAERFKATQK
VGFLKAAHRLPAGDPGRESAQIARLRRLAEDAHLDPAFAEKFLNFIIGEVIRHHEAIAED
HQAAAEWK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory