SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_059150936.1 NCBI__GCF_001046635.1:WP_059150936.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WQ37 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
32% identity, 97% coverage: 9:536/545 of query aligns to 3:495/503 of P9WQ37

query
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P9WQ37

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R9 (= R15) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.
R17 (≠ D23) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
K172 (= K207) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
R195 (≠ T230) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
R197 (≠ E232) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
V209 (≠ I244) mutation to D: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
A211 (≠ G246) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
T214 (≠ P249) mutation to W: Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
R244 (≠ G279) mutation to A: Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
A302 (≠ G340) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.; mutation to W: Does not show activity with small, medium or long acyl chains.
W377 (= W418) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
D382 (= D423) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
R397 (= R438) mutation to A: Reduction of binding affinity for fatty acids.
S404 (≠ V445) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
G406 (= G447) mutation to L: No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
K487 (= K528) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.

Q4WR83 Acyl-CoA ligase sidI; Siderophore biosynthesis protein I; EC 6.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
30% identity, 98% coverage: 2:536/545 of query aligns to 28:571/590 of Q4WR83

query
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Q4WR83

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Sites not aligning to the query:

6:14 PTS2-type peroxisomal targeting signal

3r44A Mycobacterium tuberculosis fatty acyl coa synthetase (see paper)
32% identity, 97% coverage: 9:536/545 of query aligns to 6:495/502 of 3r44A

query
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3r44A

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R

Y

L

Q

A

A

A

S

P

E

E

L

V

G

S

F

F

V

I

V

L

A

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D

D

A

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L

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V

K

A

V

I

V

I

I

T

Y

T

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G

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A

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P

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I

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D

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-

S

A

A

I

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R

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A

E

Q

A

A

A

D

M

P

P

P

S

G

L

T

T

V

Q

T

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D

G

-

D

-

P

-

W

W

A

I

L

G

S

A

L

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P

S

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L

A

A

P

E

R

R

L

L

R

R

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S

I

-

V

-

C

-

L

-

D

-

E

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C

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A

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Y

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-

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Y

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|
T

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G

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-

A

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N

H

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K

K

G

G

A

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M

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I

H

S

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H

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x
E

A

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Q

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H

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x
A

S

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L

W

G

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P

L

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|
H

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A

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A

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T

M

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M

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I

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L

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I

R

G

G

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V

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Y

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F

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D

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G

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G

-

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V

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N

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L

L

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K

G

E

Y

Y

Y

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N

N

A

R

P

P

E

E

K

A

T

T

A

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D

A

A

I

F

R

D

D

N

G

G

W

W

F

F

F

R

S

T

G

G

D

D

I

I

G

G

S

E

I

I

D

D

A

D

N

E

G

G

N

Y

V

L

M

Y

F

I

H

K

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R

T

L

K

K

D

D

M

M

L

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x
I

V

S

G

G

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E

N
|
N

V

V

A

Y

A

P

A

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A

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A

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Y

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C

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G

G

T

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I

L

A

A

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F

Y

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K

V

L

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P

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K

H

K

I

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I

F

F

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A

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E

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A

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I

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P

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N

-

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T

T

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G

K
|
K

I

I

Q

L

K

K

F

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V

L

L

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E

active site: T167 (= T199), A184 (≠ N219), H208 (= H243), T304 (= T342), E305 (= E343), I403 (≠ K444), N408 (= N449), K487 (= K528)
binding histidine: E179 (≠ R214), H182 (≠ V217)

Sites not aligning to the query:

binding histidine: 5

P69451 Long-chain-fatty-acid--CoA ligase; Long-chain acyl-CoA synthetase; Acyl-CoA synthetase; EC 6.2.1.3 from Escherichia coli (strain K12) (see paper)
29% identity, 97% coverage: 9:536/545 of query aligns to 24:551/561 of P69451

query
sites
P69451

T

S

M

L

G

V

E

D

M

M

I

F

R

E

S

Q

S

S

G

V

Q

A

R

R

F

Y

A

A

D

D

R

Q

D

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A

A

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F

V

V

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N

P

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R

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V

L

M

G

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Y

F

A

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D

K

L

L

N

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R

E

K

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A

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R

R

D

A

W

F

A

A

R

A

A

Y

L

L

A

Q

-

Q

A

G

M

L

G

G

V

L

K

K

P

K

G

G

D

D

N

R

V

V

G

A

L

L

L

M

P

P

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N

C

L

L

L

D

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F

Y

I

P

T

V

A

A

F

L

Y

F

G

G

I

I

T

L

M

R

T

A

G

G

A

M

V

I

A

V

V

V

P

N

V

V

N

N

A

P

R

L

Y

Y

Q

T

A

P

S

R

E

E

L

L

G

E

F

H

V

Q

V

L

A

N

D

D

A

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D

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L

A

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A

A

I

I

I

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I

L

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G

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-

W

-

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Q

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F

I

L

L

Q

Y
|
Y

T
|
T

S

G

G
|
G

T
|
T

T

T

A
x
G

N

V

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A

K
|
K

G

G

A

A

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M

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L

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H

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N

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L

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N

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M

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L

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I

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L

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G

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-

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-

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M

M

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W

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H

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T

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I

-

Q

-

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G

-

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G

Y

Y

G

G

L

L

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|
E

G

C

A

A

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L

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V

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D

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P

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G

G

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H

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I

L

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C

L

V

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K

G

G

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P

N

Q

V

V

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M

K

L

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G

Y

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N

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A

T

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I

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N

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G

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W

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D

D

I

I

G

A

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I

M

D

D

A

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N

E

G

G

N

F

V

L

M

R

F

I

H

V

G

D

R

R

T

K

K

K

D

D

M

M

L

I

K

L

V

V

G

S

G

G

E

F

N

N

V

V

A

Y

A

P

A

N

E

E

I

I

E

E

A

D

M

V

L

V

Q

M

T

Q

H

H

P

P

A

G

V

V

K

Q

L

E

A

V

Q

A

V

A

V

V

G

G

L

V

P

P

D

S

D

G

R

S

Y

S

A

G

E

E

V

A

P

V

A

K

A

I

F

F

V

V

E

-

L

V

A

K

E

K

G

D

A

P

A

S

A

L

S

T

E

E

Q

E

E

S

L

L

I

V

D

T

H

F

C

C

K

R

G

R

R

Q

I

L

A

T

G

G

F

Y

K

K

V

V

P

P

R

K

H

L

I

V

R

E

F

F

V

R

E

D

E

E

W

L

P

P

M

K

S

S

T

N

-

V

S

G

K

K

I

I

Q

L

K

R

F

R

R

E

L

L

R

R

E

D

Y213 (= Y198) mutation to A: Loss of activity.
T214 (= T199) mutation to A: 10% of wild-type activity.
G216 (= G201) mutation to A: Decreases activity.
T217 (= T202) mutation to A: Decreases activity.
G219 (≠ A204) mutation to A: Decreases activity.
K222 (= K207) mutation to A: Decreases activity.
E361 (= E343) mutation to A: Loss of activity.

Q9SMT7 Oxalate--CoA ligase; 4-coumarate--CoA ligase isoform 8; At4CL8; 4-coumarate--CoA ligase-like 10; Acyl-activating enzyme 3; Adenosine monophosphate binding protein 3; AtMPBP3; Oxalyl-CoA synthetase; EC 6.2.1.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 96% coverage: 9:531/545 of query aligns to 5:503/514 of Q9SMT7

query
sites
Q9SMT7

T

T

M

L

G

S

E

G

M

L

I

L

R

E

S

N

S

V

G

A

Q

K

R

K

F

F

A

P

D

D

R

R

D

R

A

A

I

L

V

S

F

V

P

S

D

G

R

K

-

F

R

N

L

L

G

T

Y

H

A

A

D

R

L

L

N

H

R

D

K

L

-

I

A

E

R

R

D

A

W

A

A

S

R

R

A

L

L

V

A

S

A

D

M

A

G

G

V

I

K

K

P

P

G

G

D

D

N

V

V

V

G

A

L

L

L

T

L

F

P

P

T

N

C

T

L

V

D

E

F

F

I

V

T

I

A

M

F

F

Y

L

G

A

I

V

T

I

M

R

T

A

G

R

A

A

V

T

A

A

V

A

P

P

V

L

N

N

A

A

R

A

Y

Y

Q

T

A

A

S

E

E

E

L

F

G

E

F

F

V

Y

V

L

A

S

D

D

A

S

D

D

L

S

V

K

A

L

I

L

T

T

T

S

G

K

K

E

-

G

V

N

A

A

D

P

G

A

L

Q

D

E

F

A

S

A

A

S

R

K

L

L

E

K

A

I

A

S

M

H

P

V

S

T

L

A

T

T

Q

-

S

-

G

-

D

-

P

-

W

-

A

-

L

-

S

-

L

-

P

-

E

-

A

-

P

-

R

-

L

-

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-

S

-

I

-

V

-

C

-

L

-

D

-

E

-

R

-

C

-

A

-

P

-

Y

-

L

L

V

L

P

D

A

A

N

G

A

S

A

D

L

L

A

V

K

L

G

S

A

V

K

A

I

D

A

S

E

D

D

S

E

V

I

V

D

D

A

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R

A

I

T

D

E

A

L

V

V

-

N

A

H

P

P

Q

D

D

D

T

G

A

A

M

L

I

F

L

L

Y

H

T
|
T

S
|
S

G
|
G

T
|
T

A

S

N

R

P

P

K

K

G

G

A

V

M

P

I

L

S

T

H

Q

R

L

A

N

Q

L

V

A

A

S

N

S

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R

K

G

N

L

I

G

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V

A

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Y

Y

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D

K

S

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T

W

V

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P

V

L

L

P

P

I

L

F

F

H
|
H

I

V

A

H

G

G

I

L

L

L

P

A

M

G

T

L

M

L

I

S

L

S

D

L

I

G

G

A

G

G

V

A

Y

A

M

V

T

T

I

L

P

P

-

A

-

G

R

R

F

F

D

S

A

A

G

T

V

T

G

F

L

W

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P

M

D

L

M

G

K

R

K

E

Y

G

N

A

A

T

T

-

W

-

Y

I

T

A

A

Y

V

P

P

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T

F

I

V

H

T

Q

I

I

M

I

Q

L

D

D

L

-

I

-

T

R

H

H

P

A

T

S

F

H

K

P

D

E

T

T

D

E

L

Y

S

P

R

K

L

L

R

R

I

F

M

I

N

R

S

S

N

C

F
x
S

A
|
A

V
x
S

Q

L

P

A

E

P

W

V

I

I

K

L

L

S

A

R

V

L

A

E

E

E

A

A

M

F

P

G

H

A

T

P

I

V

Q

-

V

L

G
x
E

T
x
A

Y

Y

G

A

L
x
M

T
|
T

E

E

G

A

A

T

G
x
H

T

L

V

M

S

S

T

S

S

N

R

P

L

L

S

P

D

E

S

E

Y

G

E

P

V

H

R

K

T

P

G

G

R

S

C

V

G

G

V

K

P

P

L

V

E

G

E

Q

W

-

Q

E

V

M

R

A

I

I

V

L

D

N

P

-

E

E

T

K

G

G

A

E

D

I

C

Q

G

E

P

P

G

N

E

N

H

K

G

G

E

E

I

V

V

C

L

I

G

R

G

G

P

P

N

N

V

V

L

T

K

K

G

G

Y

Y

Y

K

N

N

A

N

P

P

E

E

K

A

T

N

A

K

E

A

A

G

I

F

R

E

D

F

G

G

W

W

F

F

F

H

S

T

G

G

D
|
D

I

I

G

G

S

Y

I

F

D

D

A

T

N

D

G

G

N

Y

V

L

M

H

F

L

H

V

G

G

R
|
R

T

I

K

K

D

E

M

L

L

I

K

N

V

R

G

G

E

E

N

K

V

I

A

S

A

P

A

I

E

E

I

V

E

D

A

A

M

V

L

L

Q

L

T

T

H

H

P

P

A

D

V

V

K

S

L

Q

A

G

Q

V

V

A

V

F

G

G

L

V

P

P

D

D

D

E

R

K

Y

Y

A

G

E

E

V

E

P

I

A

N

A

C

F

A

V

V

E

I

L

P

A

R

E

E

G

G

A

T

A

V

S

T

E

E

Q

E

E

D

L

I

I

K

D

A

H

F

C

C

K

K

G

K

R

N

I

L

A

A

G

A

F

F

K

K

V

V

P

P

R

K

H

R

I

V

R

F

F

I

V

T

E

D

E

N

W

L

P

P

M

K

S

T

T

A

S

S

-

G

K
|
K

I

I

Q

Q

K

R

TSGTT 170:174 (= TSGTT 199:203) binding ATP
H214 (= H243) binding ATP; mutation to A: Abolished activity.
S289 (≠ F315) binding oxalate; mutation to A: Abolished activity.
SAS 289:291 (≠ FAV 315:317) binding ATP
EA 310:311 (≠ GT 337:338) binding ATP
M314 (≠ L341) binding oxalate
T315 (= T342) binding ATP
H319 (≠ G346) binding oxalate; mutation to A: Abolished activity.
D394 (= D423) binding ATP
R409 (= R438) binding ATP; mutation to A: Abolished activity.
K500 (= K528) binding ATP; binding oxalate; mutation to A: Abolished activity.

5ie3A Crystal structure of a plant enzyme (see paper)
28% identity, 96% coverage: 9:531/545 of query aligns to 5:496/504 of 5ie3A

query
sites
5ie3A

T

T

M

L

G

S

E

G

M

L

I

L

R

E

S

N

S

V

G

A

Q

K

R

K

F

F

A

P

D

D

R

R

D

R

A

A

I

L

V

S

F

V

P

S

D

G

R

K

-

F

R

N

L

L

G

T

Y

H

A

A

D

R

L

L

N

H

R

D

K

L

-

I

A

E

R

R

D

A

W

A

A

S

R

R

A

L

L

V

A

S

A

D

M

A

G

G

V

I

K

K

P

P

G

G

D

D

N

V

V

V

G

A

L

L

L

T

L

F

P

P

T

N

C

T

L

V

D

E

F

F

I

V

T

I

A

M

F

F

Y

L

G

A

I

V

T

I

M

R

T

A

G

R

A

A

V

T

A

A

V

A

P

P

V

L

N

N

A

A

R

A

Y

Y

Q

T

A

A

S

E

E

E

L

F

G

E

F

F

V

Y

V

L

A

S

D

D

A

S

D

D

L

S

V

K

A

L

I

L

T

T

T

S

G

K

K

E

-

G

V

N

A

A

D

P

G

A

L

Q

D

E

F

A

S

A

A

S

R

K

L

L

E

K

A

I

A

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-

H

-

V

M

T

P

A

S

T

L

L

T

L

Q

D

S

A

G

G

D

S

P

D

W

L

A

V

L

L

S

S

L

V

P

A

E

D

A

A

P

T

R

E

L

L

R

V

S

N

I

-

V

-

C

-

L

-

D

-

E

-

R

-

C

-

A

-

P

-

Y

-

L

-

V

-

P

-

A

-

N

-

A

-

L

-

A

-

K

-

G

-

A

-

K

-

I

-

A

-

E

-

D

-

E

-

I

-

D

-

A

-

R

-

I

-

D

-

A

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V

-

A

H

P

P

Q

D

D

D

T

G

A

A

M

L

I

F

L

L

Y

H

T
|
T

S
|
S

G

G

T

T

A

S

N

R

P

P

K

K

G

G

A

V

M

P

I

L

S

T

H

Q

R

L

A

N

Q

L

V

A

A

S

N
x
S

S

V

R

K

G

N

L

I

G

K

V

A

R

V

Y

Y

E

K

C

L

T

T

E

E

E

S

D

D

K

S

V

T

W

V

S

I

P

V

L

L

P

P

I

L

F

F

H
|
H

I
x
V

A

H

G

G

I

L

L

L

P

A

M

G

T

L

M

L

I

S

L

S

D

L

I

G

G

A

G

G

V

A

Y

A

M

V

T

T

I

L

P

P

-

A

-

G

R

R

F

F

D

S

A

A

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V

T

G

F

L

W

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P

M

D

L

M

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K

R

K

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Y

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N

A

A

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-

W

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Y

I

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F

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V

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I

M

I

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D

D

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-

I

-

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H

H

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K

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D

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T

T

D

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L

Y

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P

R

K

L

L

R

R

I

F

M

I

N

R

S

S

N

C

F
x
S

A
|
A

V
x
S

Q

L

P

A

E

P

W

V

I

I

K

L

L

S

A

R

V

L

A

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E

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A

A

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F

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G

H

A

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P

I

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Q

-

V

L

G

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T

A

Y
|
Y

G
x
A

L
x
M

T
|
T

E
|
E

G

A

A

T

G
x
H

T

L

V

M

S

S

T

S

S

N

R

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L

L

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D

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Q

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N

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T

K

G

G

A

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D

I

C

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G

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P

P

G

N

E

N

H

K

G

G

E

E

I

V

V

C

L

I

G

R

G

G

P

P

N

N

V

V

L

T

K

K

G

G

Y

Y

Y

K

N

N

A

N

P

P

E

E

K

A

T

N

A

K

E

A

A

G

I

F

R

E

D

F

G

G

W

W

F

F

F

H

S

T

G

G

D
|
D

I

I

G

G

S

Y

I

F

D

D

A

T

N

D

G

G

N

Y

V

L

M

H

F
x
L

H

V

G

G

R
|
R

T

I

K

K

D

E

M

L

L

I

K
x
N

V

R

G

G

E

E

N
x
K

V

I

A

S

A

P

A

I

E

E

I

V

E

D

A

A

M

V

L

L

Q

L

T

T

H

H

P

P

A

D

V

V

K

S

L

Q

A

G

Q

V

V

A

V

F

G

G

L

V

P

P

D

D

D

E

R

K

Y

Y

A

G

E

E

V

E

P

I

A

N

A

C

F

A

V

V

E

I

L

P

A

R

E

E

G

G

A

T

A

V

S

T

E

E

Q

E

E

D

L

I

I

K

D

A

H

F

C

C

K

K

G

K

R

N

I

L

A

A

G

A

F

F

K

K

V

V

P

P

R

K

H

R

I

V

R

F

F

I

V

T

E

D

E

N

W

L

P

P

M

K

S

T

T

A

S

S

-

G

K
|
K

I

I

Q

Q

K

R

active site: T163 (= T199), S183 (≠ N219), H207 (= H243), T308 (= T342), E309 (= E343), N408 (≠ K444), K413 (≠ N449), K493 (= K528)
binding adenosine monophosphate: S164 (= S200), S282 (≠ F315), A283 (= A316), S284 (≠ V317), Y305 (= Y339), A306 (≠ G340), M307 (≠ L341), T308 (= T342), D387 (= D423), L399 (≠ F435), R402 (= R438), K493 (= K528)
binding oxalic acid: V208 (≠ I244), S282 (≠ F315), A306 (≠ G340), M307 (≠ L341), H312 (≠ G346), K493 (= K528)

5ie2A Crystal structure of a plant enzyme (see paper)
28% identity, 96% coverage: 9:531/545 of query aligns to 5:498/506 of 5ie2A

query
sites
5ie2A

T

T

M

L

G

S

E

G

M

L

I

L

R

E

S

N

S

V

G

A

Q

K

R

K

F

F

A

P

D

D

R

R

D

R

A

A

I

L

V

S

F

V

P

S

D

G

R

K

-

F

R

N

L

L

G

T

Y

H

A

A

D

R

L

L

N

H

R

D

K

L

-

I

A

E

R

R

D

A

W

A

A

S

R

R

A

L

L

V

A

S

A

D

M

A

G

G

V

I

K

K

P

P

G

G

D

D

N

V

V

V

G

A

L

L

L

T

L

F

P

P

T

N

C

T

L

V

D

E

F

F

I

V

T

I

A

M

F

F

Y

L

G

A

I

V

T

I

M

R

T

A

G

R

A

A

V

T

A

A

V

A

P

P

V

L

N

N

A

A

R

A

Y

Y

Q

T

A

A

S

E

E

E

L

F

G

E

F

F

V

Y

V

L

A

S

D

D

A

S

D

D

L

S

V

K

A

L

I

L

T

T

T

S

G

K

K

E

-

G

V

N

A

A

D

P

G

A

L

Q

D

E

F

A

S

A

A

S

R

K

L

L

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K

A

I

A

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-

H

-

V

M

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P

A

S

T

L

L

T

L

Q

D

S

A

G

G

D

S

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D

W

L

A

V

L

L

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S

L

-

P

-

E

-

A

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P

-

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-

L

-

R

-

S

-

I

-

V

-

C

-

L

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D

-

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-

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A

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-

Y

-

L

-

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-

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-

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N

-

A

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L

-

A

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K

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G

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A

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K

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A

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D

D

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A

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P

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D

D

D

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A

A

M

L

I

F

L

L

Y

H

T
|
T

S
|
S

G
|
G

T
|
T

A

S

N

R

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P

K

K

G

G

A

V

M

P

I

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T

H

Q

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A

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Q

L

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A

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N
x
S

S

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K

G

N

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Y

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D

K

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L

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P

I

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F

F

H
|
H

I

V

A

H

G

G

I

L

L

L

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A

M

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L

M

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L

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D

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A

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L

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F

M

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N

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S

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x
S

A
|
A

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|
Y

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A

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M

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T

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|
E

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N

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V

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K

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G

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Y

Y

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N

A

N

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P

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A

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A

A

G

I

F

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D

F

G

G

W

W

F

F

F

H

S

T

G

G

D
|
D

I

I

G

G

S

Y

I

F

D

D

A

T

N

D

G

G

N

Y

V

L

M

H

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x
L

H

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G

G

R
|
R

T

I

K

K

D

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M

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L

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N

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G

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L

P

A

R

E

E

G

G

A

T

A

V

S

T

E

E

Q

E

E

D

L

I

I

K

D

A

H

F

C

C

K

K

G

K

R

N

I

L

A

A

G

A

F

F

K

K

V

V

P

P

R

K

H

R

I

V

R

F

F

I

V

T

E

D

E

N

W

L

P

P

M

K

S

T

T

A

S

S

-

G

K
|
K

I

I

Q

Q

K

R

active site: T165 (= T199), S185 (≠ N219), H209 (= H243), T310 (= T342), E311 (= E343), N410 (≠ K444), K415 (≠ N449), K495 (= K528)
binding adenosine-5'-triphosphate: T165 (= T199), S166 (= S200), G167 (= G201), T168 (= T202), T169 (= T203), S284 (≠ F315), A285 (= A316), S286 (≠ V317), Y307 (= Y339), A308 (≠ G340), M309 (≠ L341), T310 (= T342), D389 (= D423), L401 (≠ F435), R404 (= R438), K495 (= K528)

Q67W82 4-coumarate--CoA ligase 4; 4CL 4; Os4CL4; (E)-ferulate--CoA ligase; 4-coumaroyl-CoA synthase 4; Protein RESISTANCE TO ALUMINUM 1; EC 6.2.1.12; EC 6.2.1.34 from Oryza sativa subsp. japonica (Rice) (see paper)
31% identity, 93% coverage: 36:540/545 of query aligns to 62:547/559 of Q67W82

query
sites
Q67W82

Y

Y

A

G

D

E

L

V

N

E

R

E

K

L

A

S

R

R

D

R

W

A

A

A

R

A

A

G

L

L

A

R

M

L

G

G

V

V

K

G

P

K

G

G

D

D

N

V

V

V

G

M

L

S

L

L

P

R

T

N

C

C

L

P

D

E

F

F

I

A

T

F

A

T

F

F

Y

L

G

G

I

A

T

A

M

R

T

L

G

G

A

A

V

A

A

T

V

T

P

T

V

A

N

N

A

P

R

F

Y

Y

Q

T

A

P

S

H

E

E

L

I

G

H

F

R

V

Q

V

A

A

S

D

A

A

A

D

G

L

A

V

R

A

V

I

I

I

V

T

T

T

E

G

A

K

C

V

A

A

V

D

E

G

K

L

V

-

R

D

G

F

F

S

A

A

A

R

-

L

-

E

D

A

R

A

G

M

I

P

P

S

V

L

V

T

A

Q

V

S

D

G

G

D

D

-

F

P

D

W

G

A

C

L

V

S

G

L

F

P

G

E

E

A

A

P

M

R

L

L

D

R

A

S

S

I

I

V

E

C

P

L

L

D

D

E

-

R

-

C

-

A

-

P

-

Y

-

L

-

V

-

P

-

A

-

N

-

A

-

L

-

A

-

K

-

G

-

A

-

K

-

I

-

A

A

E

D

D

E

E

E

I

-

D

-

A

-

R

-

I

-

D

-

A

-

V

V

A

H

P

P

Q

D

D

D

T

V

A

V

M

A

I

L

L

P

Y

Y

T

S

S

S

G

G

T

T

A

G

N

L

P

P

K

K

G

G

A

V

M

M

I

L

S

T

H

H

R

R

A

S

Q

L

V

V

A

T

N

S

-

V

-

A

-

Q

S

V

R

D

G

G

L

E

G

N

V

P

R

N

Y

L

E

Y

C

F

T

R

E

R

E

E

D

D

K

V

V

V

W

L

S

C

P

L

L

L

P

P

I

L

F

F

H

H

I

I

A

Y

G

S

I

L

L

N

P

S

M

V

T

L

M

L

I

A

-

G

L

L

D

R

I

A

G

G

G

S

V

A

Y

I

M

V

T

I

I

M

P

R

R

K

F

F

D

D

A

L

G

G

V

A

G

L

L

V

E

D

M

L

L

T

G

R

R

R

E

H

G

G

A

V

T

T

I

V

A

A

Y

-

P

P

S

F

F

V

V

P

T

P

I

I

M

V

Q

V

D

E

L

I

I

A

T

K

H

S

P

P

T

R

F

V

K

T

D

A

T

D

D

D

L

L

S

A

R

S

L

I

R

R

I

M

M

V

N

M

S

S

N

G

F

A

A

A

V

P

Q

M

P

G

E

K

W

D

I

L

K

Q

L

D

A

A

V

F

A

M

E

A

A

K

M

I

P

P

H

N

T

A

I

V

Q

L

V

G

G

Q

T

G

Y

Y

G

G

L

M

T

T

E

E

G

-

A

A

G

G

T

P

V

V

S

L

T

A

S

M

R

C

L

L

S

A

-

F

-

A

-

K

D

E

S

P

Y

F

E

E

V

V

R

K

T

S

G

G

R

S

C

C

G

G

V

T

P

V

L

V

E

R

E

N

W

A

Q

E

V

L

R

K

I

I

V

V

D

D

P

P

E

D

T

T

G

G

A

A

D

T

C

L

G

G

P

R

G

N

E

Q

H

S

G
|
G

E

E

I

I

V

C

L

I

G

R

G

G

P

E

N

Q

V

I

L

M

K

K

G

G

Y

Y

Y

L

N

N

A

D

P

P

E

E

K

S

T

T

A

K

E

N

A

T

I

I

-

D

R

K

D

G

G

G

W

W

F

L

F

H

S

T

G

G

D

D

I

I

G

G

S

Y

I

V

D

D

A

D

N

D

G

D

N

E

V

I

M

F

F

I

H

V

G

D

R

R

T

L

K

K

D

E

M

I

L

I

K

K

V

Y

G

K

G

G

E

F

N

Q

V

V

A

P

A

A

E

E

I

L

E

E

A

A

M

L

L

L

Q

I

T

T

H

H

P

P

A

D

V

I

K

K

L

D

A

A

Q

A

V

V

G

P

L

M

P

I

D

D

D

E

R

I

Y

A

A

G

E

E

V

V

P

P

A

V

A

A

F

F

V

I

E

V

L

R

A

I

E

E

G

G

A

S

A

A

A

I

S

S

E

E

Q

N

E

E

L

I

I

K

D

Q

H

F

C

V

K

A

G

K

R

E

I

V

A

V

G

F

F

Y

K

K

V

R

P

L

R

N

H

K

I

V

R

F

F

F

V

A

E

D

E

S

W

I

P

P

M

K

S

S

T

P

S

S

-

G

K

K

I

I

Q

L

K

R

F

K

R

D

L

L

R

R

E

A

G

K

L

L

I

A

A

A

G395 (= G390) mutation to R: In ral1; reduced lignin content and increased accumulation of 4-coumarate and ferulate in roots; confers increased tolerance to aluminum.

O24146 4-coumarate--CoA ligase 2; 4CL 2; Nt4CL-19; Nt4CL-2; 4-coumaroyl-CoA synthase 2; Caffeate--CoA ligase; Cinnamate--CoA ligase; Ferulate--CoA ligase; EC 6.2.1.12; EC 6.2.1.-; EC 6.2.1.-; EC 6.2.1.34 from Nicotiana tabacum (Common tobacco) (see paper)
30% identity, 93% coverage: 36:542/545 of query aligns to 55:540/542 of O24146

query
sites
O24146

Y

Y

A

A

D

D

L

V

N

E

R

L

K

N

A

S

R

R

D

K

W

V

A

A

R

A

A

G

L

L

A

H

A

K

M

Q

G

G

V

I

K

Q

P

P

G

K

D

D

N

T

V

I

G

M

L

I

L

L

P

P

T

N

C

S

L

P

D

E

F

F

I

V

T

F

A

A

F

F

Y

I

G

G

I

A

T

S

M

Y

T

L

G

G

A

A

V

I

A

S

V

T

P

M

V

A

N

N

A

P

R

L

Y

F

Q

T

A

P

S

A

E

E

L

-

G

-

F

-

V

-

A

-

D

-

A

-

D

-

L

-

V

-

A

-

I

V

T

K

T

Q

G

A

K

K

V

A

A

S

D

-

G

-

L

-

D

-

F

-

S

S

A

A

R

K

L

I

E

I

A

V

A

T

M

Q

P

A

S

C

L

H

T

V

Q

N

S

K

G

V

D

K

P

D

W

Y

A

A

L

F

S

E

L

-

P

-

E

-

A

-

P

-

R

N

L

D

R

V

S

K

I

I

V

I

C

C

L

I

D

D

-

S

-

A

-

P

E

E

R

G

C

C

A

L

P

H

Y

F

L

S

V

V

P

L

A

T

N

Q

A

A

A

-

L

-

A

-

K

-

G

-

A

-

K

-

I

-

A

-

E

-

D

N

E

E

I

H

D

D

A

I

R

P

I

E

D

V

A

E

V

I

A

Q

P

P

Q

D

D

D

T

V

A

V

M

A

I

L

L

P

Y

Y

T
x
S

S
|
S

G
|
G

T
|
T

A

G

N

L

P

P

K
|
K

G

G

A

V

M

M

I

L

S

T

H

H

R

K

A

G

Q

L

V

V

A

T

N

S

S

V

-

A

-

Q

-

V

R

D

G

G

L

E

G

N

V

P

R

N

Y

L

E

Y

C

I

T

H

E

S

E

E

D

D

K

V

V

M

W

L

S

C

P

V

L

L

P

P

I

L

F

F

H
|
H

I

I

A
x
Y

G

S

I

L

L

N

P
x
S

M

V

T

L

M

L

I

C

-

G

L

L

D

R

I

V

G

G

G

A

V

A

Y

I

M

L

T

I

I

M

P

Q

R
x
K

F

F

D

D

A

I

G

V

V

S

G

F

L

L

E

E

M

L

L

I

G

Q

R

R

E

Y

G

K

A

V

T

T

I

I

A

G

Y

-

P

P

S

F

F

V

V

P

T

P

I

I

M

V

Q

L

D

A

L

I

I

A

T

K

H

S

P

P

T

M

F

V

K

D

D

D

T

Y

D

D

L

L

S

S

R

S

L

V

R

R

I

T

M

V

N

M

S

S

N

G

F
x
A

A

A

V

P

Q

L

P

G

E

K

W

E

I

L

K

E

L

D

A

T

V

V

A

R

E

A

A

K

M

F

P

P

H

N

T

A

I

K

Q

L

V

G

G
x
Q

T
x
G

Y

Y

G

G

L

M

T
|
T

E

E

G

-

A

A

G

G

T

P

V
|
V

S

L

T

A

S
x
M

R

C

L

L

S

A