SitesBLAST

R102 (= R144) mutation to A: Loss of transport activity.
I105 (≠ Q147) mutation to S: Affects symport activity. May function as an uniporter.
E122 (= E164) mutation to A: Loss of transport activity.
Q137 (≠ G185) mutation to A: Loss of transport activity.
Q250 (≠ D279) mutation to A: Loss of transport activity.
Q251 (≠ Y280) mutation to A: Loss of transport activity.
N256 (vs. gap) mutation to A: Loss of transport activity.
W357 (≠ P377) mutation to A: Loss of transport activity.

Sites not aligning to the query:

22 D→N: Affects symport activity. May function as an uniporter.

P25297 Inorganic phosphate transporter PHO84 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
27% identity, 32% coverage: 91:250/501 of query aligns to 117:301/587 of P25297

query
sites
P25297

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C145 (≠ G119) mutation to S: No significant effect on transport activity; when associated with S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
F160 (≠ S131) mutation to C: Reduces phosphate binding, transport and signaling activities.
R168 (= R144) mutation to A: Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates.; mutation to C: Abolishes transport and signaling activities.; mutation to E: Reduces transport activity. Moderately reduces affinity for inorganic phosphate. No significant effects on growth rates.; mutation to Q: Reduces transport activity. Moderately reduces affinity for inorganic phosphate. Moderately reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
G172 (= G148) mutation to C: Abolishes transport and signaling activities.
G174 (≠ S150) mutation to C: Abolishes transport and signaling activities.
G176 (= G152) mutation to C: Abolishes transport and signaling activities.
D178 (≠ E154) mutation D->E,N: Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on signaling activities. No significant effects on growth rates.
Y179 (= Y155) mutation to A: Significantly reduces transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Slightly reduces signaling activity.; mutation to G: Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Abolishes signaling activity.; mutation Y->S,F: No significant effects on transport activity. No significant effects on growth rates under low phosphate conditions. Does not affect patterns of secreted phosphatase activities under low and high phosphate conditions. No significant effects on signaling activity.
C237 (≠ G209) mutation to S: No significant effect on transport activity; when associated with S-145, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
C241 (vs. gap) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
C245 (vs. gap) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
C263 (≠ G224) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
K298 (≠ R247) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); mutation to A: No significant effect on membrane localization under low phosphate conditions and internalization after phosphate addition. Strongly affects vacuolar sorting of the protein after internalization.

Sites not aligning to the query:

6 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
304:327 mutation Missing: Increases transport activity. Has no significant effect on membrane localization under low phosphate conditions. Results in severely delayed internalization after phosphate addition.
335 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-399, S-434, S-455, S-474, S-510 and S-519.
358 D→E: Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Moderately decreases signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.; D→N: Significantly reduces transport activity. No significant effects on affinity for inorganic phosphate. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. No significant effects on signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
392 V→C: Reduces transport and signaling activities.
399 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-434, S-455, S-474, S-510 and S-519.
408 V→C: Reduces signaling activity and enhances transport activity.
434 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-455, S-474, S-510 and S-519.
455 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-474, S-510 and S-519.
473 E→K: No significant effects on transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates.; E→Q: No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates.
474 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-510 and S-519.
492 mutation K->A,Q: No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates.; K→E: Reduces transport activity. Reduces affinity for inorganic phosphate. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
510 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474 and S-519.
519 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474 and S-510.

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
25% identity, 56% coverage: 49:327/501 of query aligns to 56:315/444 of Q8NLB7

query
sites
Q8NLB7

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G

D57 (= D50) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R102) mutation to A: Loss of transport activity.
W309 (≠ I321) mutation to V: Loss of transport activity.
D312 (= D324) mutation to A: Loss of transport activity.
R313 (= R325) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

Query Sequence

>WP_059151316.1 NCBI__GCF_001046635.1:WP_059151316.1
MSSIRAPQAHLGWFKRRREMKVEDVTLVDRSTLNRAVGAAALGNAMEWFDFGVYGYIAVT
LGRVFFPTGNPTAQLIATLATFTAAFLVRPLGGIVFGPLGDRYGRHKVLAFTMIMMAIGT
FSIGLIPSYNSIGMAAPALLLAARLVQGFSTGGEYGGAATFIAEYSTDKKRGLMGSWLEF
GTLGGYIAGAGTVTALQMSLSDADMLAWGWRLPFLIAGPLGLLGLYMRLKLEETPAFQAY
SEEAEKREMDRPGLGDLFRVHWRQLLKCVGLVLVFNVTDYMLLTYMPSYLSVTMGYAESK
GLLLIILVMLVMMPLNIVGGIFSDRLGRKPMIIGACTALLVLAVPCMLLIGTGNNWLIFL
GLMLLGVALVCFTSSMPSTLPALFYTPVRYSGLSIAFNVSVSLFGGTTPLFTAWLVDVTG
DPLVPAYYLMVAAAIGIFTMLTVRETAGMPLRGSPPAVETKAEARALMAGDQPVTVDRVL
PGSNSPAVTPVLKPTPRHALI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory