SitesBLAST
Comparing WP_059151561.1 NCBI__GCF_001046635.1:WP_059151561.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P76558 NADP-dependent malic enzyme; NADP-ME; EC 1.1.1.40 from Escherichia coli (strain K12) (see paper)
56% identity, 99% coverage: 1:748/757 of query aligns to 1:751/759 of P76558
- K56 (= K56) modified: N6-acetyllysine
6zngF Maeb full-length acetyl-coa bound state (see paper)
47% identity, 98% coverage: 4:742/757 of query aligns to 3:734/753 of 6zngF
- active site: Y38 (= Y39), A74 (= A75), K93 (= K94), E135 (= E136), D136 (= D137), D160 (= D161), D161 (= D162), N286 (= N286)
- binding acetyl coenzyme *a: R511 (≠ A516), K514 (= K519), Y552 (= Y558), A553 (≠ W559), R557 (≠ E563), L560 (= L566), P571 (≠ V578), T590 (≠ H597), V591 (≠ M598), N592 (≠ V599), L593 (≠ P600), Y625 (≠ H632), Q659 (≠ H667), L690 (= L698), N694 (= N702), Q724 (≠ S732)
6zn4A Maeb malic enzyme domain apoprotein (see paper)
60% identity, 53% coverage: 4:406/757 of query aligns to 2:405/406 of 6zn4A
6zn7A Maeb malic enzyme domain apoprotein (see paper)
60% identity, 53% coverage: 4:406/757 of query aligns to 2:405/405 of 6zn7A
- active site: Y37 (= Y39), A73 (= A75), K92 (= K94), E134 (= E136), D135 (= D137), D159 (= D161), D160 (= D162), N285 (= N286)
- binding magnesium ion: E134 (= E136), D135 (= D137), D160 (= D162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T164 (= T166), N191 (≠ S193), A193 (= A195), G194 (= G196), A195 (= A197), S196 (≠ A198), D218 (= D220), S219 (≠ K221), K235 (≠ M236), L260 (= L261), S261 (= S262), V262 (≠ A263), M283 (≠ L284), N285 (= N286), V315 (= V316)
5ceeA Malic enzyme from candidatus phytoplasma aywb in complex with NAD and mg2+ (see paper)
46% identity, 49% coverage: 31:403/757 of query aligns to 26:385/387 of 5ceeA
- active site: Y34 (= Y39), A70 (= A75), K89 (= K94), E131 (= E136), D132 (= D137), D156 (= D161), D157 (= D162), N283 (= N286)
- binding magnesium ion: E131 (= E136), D132 (= D137), D157 (= D162)
- binding nicotinamide-adenine-dinucleotide: T161 (= T166), N188 (≠ S193), G189 (= G194), G191 (= G196), A193 (= A198), D213 (= D220), K214 (= K221), V258 (≠ L261), S259 (= S262), I263 (≠ V266), L281 (= L284), N283 (= N286), V312 (= V316), N314 (= N318)
2dvmA NAD complex structure of ph1275 protein from pyrococcus horikoshii
42% identity, 58% coverage: 5:442/757 of query aligns to 1:435/438 of 2dvmA
- active site: Y37 (= Y39), R73 (≠ A75), K92 (= K94), E134 (= E136), D135 (= D137), D159 (= D161), D160 (= D162), N296 (= N286)
- binding nicotinamide-adenine-dinucleotide: T164 (= T166), G194 (= G196), A195 (= A197), A196 (= A198), V217 (≠ T219), E218 (≠ D220), L219 (vs. gap), P224 (≠ D222), F269 (≠ L261), T270 (≠ S262), L294 (= L284), N296 (= N286), N327 (= N318)
2a9fA Crystal structure of a putative malic enzyme ((s)-malate:nad+ oxidoreductase (decarboxylating))
50% identity, 45% coverage: 19:358/757 of query aligns to 18:354/383 of 2a9fA
2haeD Crystal structure of a putative malic enzyme (malate oxidoreductase)
44% identity, 52% coverage: 9:398/757 of query aligns to 2:373/373 of 2haeD
2haeB Crystal structure of a putative malic enzyme (malate oxidoreductase)
44% identity, 52% coverage: 9:398/757 of query aligns to 2:373/373 of 2haeB
- active site: Y31 (= Y39), A67 (= A75), K86 (= K94), E128 (= E136), D129 (= D137), D153 (= D161), D154 (= D162), N280 (= N286)
- binding nicotinamide-adenine-dinucleotide: T158 (= T166), N185 (≠ S193), G188 (= G196), A189 (= A197), A190 (= A198), D210 (= D220), R211 (≠ K221), V255 (≠ L261), S256 (= S262), R257 (≠ A263), L278 (= L284), A279 (= A285), N280 (= N286), N311 (= N318)
1xcoD Crystal structure of a phosphotransacetylase from bacillus subtilis in complex with acetylphosphate (see paper)
31% identity, 41% coverage: 438:746/757 of query aligns to 15:319/325 of 1xcoD