SitesBLAST

Q29551 Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial; SCOT; 3-oxoacid CoA-transferase 1; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; SCOT-s; Succinate-coenzyme A transferase; Succinyl-CoA:3-oxoacid CoA transferase; EC 2.8.3.5 from Sus scrofa (Pig) (see 3 papers)
53% identity, 95% coverage: 10:214/216 of query aligns to 302:506/520 of Q29551

query
sites
Q29551

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E344 (= E52) active site, 5-glutamyl coenzyme A thioester intermediate

Sites not aligning to the query:

1:39 modified: transit peptide, Mitochondrion
67 C→A: Decreases KM for acetoacetyl-CoA 3-fold. No effect on KM for acetoacetate, succinyl-CoA and succinate.; C→S: Decreases KM for acetoacetate and acetoacetyl-CoA 2-fold. Increases KM for succinate 2-fold. Decreases KM for succinyl-CoA nearly 4-fold. Strongly reduces catalytic activity.
235 C→S: Similar specific activity to wild-type.

3k6mC Dynamic domains of succinyl-coa:3-ketoacid-coenzyme a transferase from pig heart. (see paper)
53% identity, 95% coverage: 10:214/216 of query aligns to 249:453/466 of 3k6mC

query
sites
3k6mC

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binding glycerol: I270 (= I31), E291 (= E52), A313 (= A74), V377 (= V138), A380 (≠ V141), T382 (vs. gap), C412 (≠ V172), N414 (≠ D174)

Sites not aligning to the query:

binding glycerol: 25, 52, 76, 99

3oxoE Succinyl-coa:3-ketoacid coa transferase from pig heart covalently bound to coa (see paper)
54% identity, 92% coverage: 16:214/216 of query aligns to 251:449/462 of 3oxoE

query
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3oxoE

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binding coenzyme a: I266 (= I31), G267 (= G32), I268 (= I33), E287 (= E52), G343 (= G108), M345 (= M110), N355 (= N120), I358 (= I123), M366 (= M131), A369 (= A134), K389 (= K153)

8i3yD Crystal structure of asct from trypanosoma brucei in complex with succinyl-coa.
56% identity, 93% coverage: 10:210/216 of query aligns to 257:455/471 of 8i3yD

query
sites
8i3yD

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Sites not aligning to the query:

binding calcium ion: 201, 229

8i40A Crystal structure of asct from trypanosoma brucei in complex with coa.
56% identity, 93% coverage: 10:210/216 of query aligns to 253:451/467 of 8i40A

query
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8i40A

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binding coenzyme a: I274 (= I31), I276 (= I33), E295 (= E52), G351 (= G108), L353 (≠ M110), N363 (= N120), G376 (= G133), K401 (= K158)

8i3yA Crystal structure of asct from trypanosoma brucei in complex with succinyl-coa.
56% identity, 96% coverage: 4:210/216 of query aligns to 242:445/459 of 8i3yA

query
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8i3yA

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T

T

binding succinyl-coenzyme a: G267 (= G30), I268 (= I31), G269 (= G32), E289 (= E52), A311 (= A74), K313 (= K76), N357 (= N120), G369 (= G132), G370 (= G133), A371 (= A134), N389 (≠ S152), K390 (= K153), K395 (= K158)

Sites not aligning to the query:

Query Sequence

>WP_059152686.1 NCBI__GCF_001046635.1:WP_059152686.1
MSEESKGWTRNEMAARAAKELQDGFYVNLGIGIPTLVANYVPKDITVTLQSENGMLGIGP
FPYPDEVDPDLINAGKQTISELPHSAYFDSAASFGMIRGGHIDLTVLGAMEVAENGDIAN
WMIPGKMIKGMGGAMDLVAGVKKIIVVMEHTSKNGDPKFIPSCTLPLTGRNVVDMVITDL
AVFQRPDHDSPFKLVEMAPGVTPEEIAAKTTAHYVS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory