SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_059152772.1 NCBI__GCF_001046635.1:WP_059152772.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6zngF Maeb full-length acetyl-coa bound state (see paper)
48% identity, 98% coverage: 11:749/754 of query aligns to 5:740/753 of 6zngF

query
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6zngF

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active site: Y38 (= Y44), A74 (= A80), K93 (= K99), E135 (= E141), D136 (= D142), D160 (= D166), D161 (= D167), N286 (= N291)
binding acetyl coenzyme *a: R511 (≠ L519), K514 (≠ R522), Y552 (≠ F561), A553 (= A562), R557 (≠ K566), L560 (≠ R569), P571 (= P580), T590 (= T599), V591 (≠ I600), N592 (= N601), L593 (≠ E602), Y625 (= Y634), Q659 (≠ A669), L690 (= L699), N694 (= N703), Q724 (≠ P733)

P76558 NADP-dependent malic enzyme; NADP-ME; EC 1.1.1.40 from Escherichia coli (strain K12) (see paper)
48% identity, 96% coverage: 12:732/754 of query aligns to 7:734/759 of P76558

query
sites
P76558

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T

I

V

F

I

A

G

S

A

G

I

L

M

L

V

K

Q

L

R

G

G

A

E

G

A

D

D

A

A

M

M

I

I

T

C

G

G

M

T

T

V

R

G

P

D

F

Y

A

H

Q

E

S

H

M

F

K

S

E

V

V

V

R

K

R

N

A

V

L

F

D

G

P

Y

A

R

E

D

G

G

K

V

L

H

P

T

F

A

G

G

-

A

I

M

H

N

M

A

L

L

V

L

G

L

K

P

N

S

H

G

T

N

V

T

F

F

M

I

A

A

D

D

T

T

T

Y

I

V

N

N

E

D

R

E

P

P

S

D

A

A

E

E

E

E

L

L

A

A

V

E

I

I

A

T

T

L

E

M

T

A

A

A

A

E

V

T

A

V

R

R

K

R

L

F

G

G

H

I

E

E

P

P

R

R

V

V

A

A

F

L

L

L

S

S

Y

H

S

S

T

N

F

F

G

G

N

S

P

S

A

D

G

C

K

P

W

S

L

S

D

S

A

K

T

M

R

R

E

Q

A

A

I

L

H

E

I

L

L

V

D

R

Q

E

Q

R

Q

A

P

P

D

E

F

L

E

M

Y

I

E

D

G

G

E

E

L

M

A

H

P

G

D

D

A

A

A

A

L

L

N

V

P

E

K

A

V

I

M

R

A

N

-

D

L

R

Y

M

P

P

F

D

S

S

R

S

L

L

T

K

A

G

P

S

A

A

N

N

V

I

L

L

I

V

M

M

P

P

G

N

L

M

Q

E

S

A

A

A

N

R

I

I

S

S

A

Y

K

N

L

L

L

L

R

R

E

V

I

S

G

S

G

S

T

E

-

G

T

V

S

T

I

V

G

G

P

P

M

V

L

L

L

M

G

G

M

V

E

A

K

K

P

P

V

V

Q

H

I

V

V

L

K56 (≠ T61) modified: N6-acetyllysine

6zn7A Maeb malic enzyme domain apoprotein (see paper)
64% identity, 53% coverage: 11:411/754 of query aligns to 4:405/405 of 6zn7A

query
sites
6zn7A

D

D

R

Q

E

E

A

A

L

L

F

L

Y

Y

H

H

S

Q

T

Q

I

G

R

K

P

P

G

G

K

K

I

I

E

E

I

V

I

I

A

S

S

S

K

K

P

P

M

C

A

A

T

T

Q

E

R

K

D

D

L

L

S

S

L

L

A

A

Y
|
Y

S

S

P

P

G

G

V

V

A

A

V

A

P

P

V

C

Q

K

A

A

I

I

A

A

D

K

D

D

P

P

A

A

T

K

A

V

Y

Y

D

D

Y

Y

T

T

A

A

K

K

G

G

N

N

L

L

V

V

A

A

V

V

I

I

S

S

N

N

G

G

T

T

A
|
A

I

V

L

L

G

G

M

L

G

G

N

N

L

I

G

G

A

P

L

A

A

A

S

G

K

K

P

P

V

V

M

M

E

E

G

G

K
|
K

A

G

V

I

L

L

F

F

K

K

R

Q

F

F

A

A

D

G

V

I

D

D

S

V

I

F

D

D

I

I

E

E

L

V

A

A

S

A

E

T

D

D

T

V

D

D

A

V

L

F

I

C

E

N

A

A

I

V

A

R

M

V

M

L

E

E

P

P

S

T

F

F

G

G

G

G

I

I

N

N

L

L

E
|
E

D
|
D

I

I

K

K

A

A

P

P

E

E

C

C

F

F

I

E

I

I

E

E

Q

E

A

R

L

L

R

K

E

K

R

E

M

M

N

N

I

I

P

P

V

V

M

F

H

H

D
|
D

D
|
D

Q

Q

H

H

G

G

T
|
T

A

A

I

I

I

V

A

S

A

G

A

A

G

A

L

L

I

L

N

N

A

A

C

C

L

S

I

I

T

T

G

N

R

R

K

K

L

M

D

E

E

T

V

V

K

R

V

I

V

V

V

V

N
|
N

G

G

A
|
A

G
|
G

A
|
A

S
|
S

A

A

L

N

A

S

C

C

T

A

S

K

L

I

I

F

K

I

S

A

M

L

G

G

V

A

R

R

H

R

E

E

N

N

V

I

L

I

V

M

C

C

D
|
D

T
x
S

R

Q

G

G

V

V

I

I

Y

Y

P

K

G

G

R

R

E

T

-

A

N

G

V

M

D

N

Q

K

F

Y

K
|
K

S

E

A

Y

H

F

A

A

V

S

M

E

T

T

D

E

K

A

R

R

T

T

L

L

L

T

E

E

A

A

I

L

E

R

G

G

A

A

D

D

V

V

V

F

L

V

G

G

L
|
L

S
|
S

A
x
V

K

A

G

G

A

A

I

L

T

T

Q

P

D

E

M

M

V

L

K

K

R

D

M

M

A

A

P

K

E

D

P

P

I

I

I

I

F

F

A

A

M
|
M

A

A

N
|
N

P

P

D

E

P

P

E

E

I

I

T

T

P

P

P

D

E

K

A

A

K

R

A

A

V

A

R

R

P

P

D

D

A

A

I

I

V

I

A

A

T

T

G

G

R

R

S

S

D

D

Y

Y

P

P

N

N

Q

Q

V
|
V

N

N

N

N

V

V

L

L

G

G

F

F

P

P

F

S

I

I

F

F

R

R

G

G

A

A

L

L

D

D

V

T

R

R

A

S

T

T

A

Q

I

I

N

N

E

E

E

E

M

M

K

K

I

L

A

A

A

A

A

V

T

H

A

A

I

L

A

A

E

K

L

L

A

A

R

R

E

E

Q

D

V

V

H

P

E

D

D

K

V

V

A

S

A

A

A

T

Y

Y

G

G

G

G

E

K

T

S

Q

F

V

K

F

F

G

G

R

R

D

D

Y

Y

I

L

I

I

P

P

A

K

P

P

F

F

D

D

P

T

R

R

L

V

M

L

E

L

V

W

V

V

P

A

M

P

A

E

V

V

A

A

K

K

A

A

A

A

M

M

E

K

S

S

G

G

V

V

A

A

Q

T

K

R

active site: Y37 (= Y44), A73 (= A80), K92 (= K99), E134 (= E141), D135 (= D142), D159 (= D166), D160 (= D167), N285 (= N291)
binding magnesium ion: E134 (= E141), D135 (= D142), D160 (= D167)
binding nadp nicotinamide-adenine-dinucleotide phosphate: T164 (= T171), N191 (= N198), A193 (= A200), G194 (= G201), A195 (= A202), S196 (= S203), D218 (= D225), S219 (≠ T226), K235 (= K241), L260 (= L266), S261 (= S267), V262 (≠ A268), M283 (= M289), N285 (= N291), V315 (= V321)

6zn4A Maeb malic enzyme domain apoprotein (see paper)
64% identity, 53% coverage: 11:411/754 of query aligns to 4:405/406 of 6zn4A

query
sites
6zn4A

D

D

R

Q

E

E

A

A

L

L

F

L

Y

Y

H

H

S

Q

T

Q

I

G

R

K

P

P

G

G

K

K

I

I

E

E

I

V

I

I

A

S

S

S

K

K

P

P

M

C

A

A

T

T

Q

E

R

K

D

D

L

L

S

S

L

L

A

A

Y
|
Y

S

S

P

P

G

G

V

V

A

A

V

A

P

P

V

C

Q

K

A

A

I

I

A

A

D

K

D

D

P

P

A

A

T

K

A

V

Y

Y

D

D

Y

Y

T

T

A

A

K

K

G

G

N

N

L

L

V

V

A

A

V

V

I

I

S

S

N

N

G

G

T

T

A
|
A

I

V

L

L

G

G

M

L

G

G

N

N

L

I

G

G

A

P

L

A

A

A

S

G

K

K

P

P

V

V

M

M

E

E

G

G

K
|
K

A

G

V

I

L

L

F

F

K

K

R

Q

F

F

A

A

D

G

V

I

D

D

S

V

I

F

D

D

I

I

E

E

L

V

A

A

S

A

E

T

D

D

T

V

D

D

A

V

L

F

I

C

E

N

A

A

I

V

A

R

M

V

M

L

E

E

P

P

S

T

F

F

G

G

G

G

I

I

N

N

L

L

E
|
E

D
|
D

I

I

K

K

A

A

P

P

E

E

C

C

F

F

I

E

I

I

E

E

Q

E

A

R

L

L

R

K

E

K

R

E

M

M

N

N

I

I

P

P

V

V

M

F

H

H

D
|
D

D
|
D

Q

Q

H

H

G

G

T

T

A

A

I

I

I

V

A

S

A

G

A

A

G

A

L

L

I

L

N

N

A

A

C

C

L

S

I

I

T

T

G

N

R

R

K

K

L

M

D

E

E

T

V

V

K

R

V

I

V

V

V

V

N

N

G

G

A

A

G

G

A

A

S

S

A

A

L

N

A

S

C

C

T

A

S

K

L

I

I

F

K

I

S

A

M

L

G

G

V

A

R

R

H

R

E

E

N

N

V

I

L

I

V

M

C

C

D

D

T

S

R

Q

G

G

V

V

I

I

Y

Y

P

K

G

G

R

R

E

T

-

A

N

G

V

M

D

N

Q

K

F

Y

K

K

S

E

A

Y

H

F

A

A

V

S

M

E

T

T

D

E

K

A

R

R

T

T

L

L

L

T

E

E

A

A

I

L

E

R

G

G

A

A

D

D

V

V

V

F

L

V

G

G

L

L

S

S

A

V

K

A

G

G

A

A

I

L

T

T

Q

P

D

E

M

M

V

L

K

K

R

D

M

M

A

A

P

K

E

D

P

P

I

I

I

I

F

F

A

A

M

M

A

A

N
|
N

P

P

D

E

P

P

E

E

I

I

T

T

P

P

P

D

E

K

A

A

K

R

A

A

V

A

R

R

P

P

D

D

A

A

I

I

V

I

A

A

T

T

G

G

R

R

S

S

D

D

Y

Y

P

P

N

N

Q

Q

V

V

N

N

N

N

V

V

L

L

G

G

F

F

P

P

F

S

I

I

F

F

R

R

G

G

A

A

L

L

D

D

V

T

R

R

A

S

T

T

A

Q

I

I

N

N

E

E

E

E

M

M

K

K

I

L

A

A

A

A

A

V

T

H

A

A

I

L

A

A

E

K

L

L

A

A

R

R

E

E

Q

D

V

V

H

P

E

D

D

K

V

V

A

S

A

A

A

T

Y

Y

G

G

G

G

E

K

T

S

Q

F

V

K

F

F

G

G

R

R

D

D

Y

Y

I

L

I

I

P

P

A

K

P

P

F

F

D

D

P

T

R

R

L

V

M

L

E

L

V

W

V

V

P

A

M

P

A

E

V

V

A

A

K

K

A

A

A

A

M

M

E

K

S

S

G

G

V

V

A

A

Q

T

K

R

active site: Y37 (= Y44), A73 (= A80), K92 (= K99), E134 (= E141), D135 (= D142), D159 (= D166), D160 (= D167), N285 (= N291)
binding magnesium ion: E134 (= E141), D135 (= D142), D160 (= D167)

5ceeA Malic enzyme from candidatus phytoplasma aywb in complex with NAD and mg2+ (see paper)
45% identity, 49% coverage: 39:410/754 of query aligns to 29:387/387 of 5ceeA

query
sites
5ceeA

D

D

L

L

S

S

L

L

A

V

Y
|
Y

S

T

P

P

G

G

V

V

A

A

V

E

P

P

V

C

Q

L

A

K

I

I

A

K

D

E

D

N

P

P

A

S

T

D

A

V

Y

Y

D

R

Y

Y

T

T

A

M

K

K

G

G

N

N

L

M

V

V

A

G

V

V

I

I

S

T

N

N

G

G

T

T

A
|
A

I

V

L

L

G

G

M

L

G

G

N

N

L

I

G

G

A

P

L

K

A

A

S

S

K

L

P

P

V

V

M

M

E

E

G

G

K
|
K

A

A

V

I

L

L

F

F

K

K

R

E

F

L

A

A

D

G

V

I

D

D

S

S

I

F

D

P

I

I

E

C

L

I

A

D

S

S

E

T

D

D

T

S

D

Q

A

E

L

I

I

V

E

N

A

I

I

V

A

S

M

K

M

I

E

S

P

T

S

V

F

F

G

G

G

A

I

I

N

N

L

L

E
|
E

D
|
D

I

I

K

K

A

S

P

P

E

Q

C

C

F

I

I

E

I

I

E

E

Q

D

A

A

L

L

R

K

E

A

R

K

M

L

N

D

I

I

P

P

V

V

M

F

H

H

D
|
D

D
|
D

Q

Q

H

H

G

G

T
|
T

A

A

I

I

I

V

A

V

A

A

A

A

G

G

L

I

I

L

N

N

A

A

C

L

L

K

I

V

T

V

G

K

R

K

K

S

L

I

D

E

E

D

V

V

K

Q

V

V

V

V

V

I

N
|
N

G
|
G

A

A

G
|
G

A

S

S
x
A

A

G

L

M

A

A

C

I

T

A

S

K

L

M

I

L

K

L

S

L

M

L

G

K

V

V

R

N

H

-

E

-

N

N

V

V

L

V

V

L

C

V

D
|
D

T
x
K

R

T

G

G

V

T

I

L

Y

Y

P

K

G

G

R

V

E

A

N

N

V

L

D

N

Q

E

F

P

K

Q

S

K

A

K

H

L

A

V

V

E

M

V

T

T

D

N

K

K

R

Y

-

Q

-

E

-

K

-

G

T

T

L

L

L

K

E

E

A

V

I

L

E

K

G

G

A

K

D

D

V

I

V

F

L

I

G

G

L
x
V

S
|
S

A

A

K

P

G

G

A
x
I

I

V

T

T

Q

A

D

E

M

M

V

V

K

A

R

T

M

M

A

A

P

K

E

D

P

A

I

I

I

V

F

F

A

A

M
x
L

A

A

N
|
N

P

P

D

V

P

P

E

E

I

I

T

M

P

P

P

D

E

E

A

A

K

K

A

K

V

-

R

G

P

G

D

A

A

R

I

I

V

V

A

A

T

T

G

G

R

R

S

S

D

D

Y

F

P

P

N

N

Q

Q

V
|
V

N

N

N
|
N

V

C

L

L

G

A

F

F

P

P

F

G

I

V

F

F

R

R

G

G

A

T

L

L

D

D

V

A

R

K

A

A

T

T

A

Q

I

I

N

T

E

E

E

E

M

M

K

K

I

K

A

A

A

A

A

T

T

Y

A

A

I

L

A

K

E

N

L

I

A

I

R

K

E

E

Q

-

V

-

H

-

E

-

D

-

V

-

A

-

A

-

A

-

Y

-

G

-

G

-

E

-

T

-

Q

Q

V

D

F

L

G

N

R

E

D

N

Y

N

I

I

I

L

P

P

A

T

P

S

F

F

D

N

P

K

R

E

L

V

M

V

E

K

V

Q

V

I

P

A

M

L

A

A

V

V

A

C

K

K

A

V

A

A

M

K

E

E

S

T

G

G

V

V

A

V

Q

R

active site: Y34 (= Y44), A70 (= A80), K89 (= K99), E131 (= E141), D132 (= D142), D156 (= D166), D157 (= D167), N283 (= N291)
binding magnesium ion: E131 (= E141), D132 (= D142), D157 (= D167)
binding nicotinamide-adenine-dinucleotide: T161 (= T171), N188 (= N198), G189 (= G199), G191 (= G201), A193 (≠ S203), D213 (= D225), K214 (≠ T226), V258 (≠ L266), S259 (= S267), I263 (≠ A271), L281 (≠ M289), N283 (= N291), V312 (= V321), N314 (= N323)

2a9fA Crystal structure of a putative malic enzyme ((s)-malate:nad+ oxidoreductase (decarboxylating))
50% identity, 44% coverage: 24:357/754 of query aligns to 18:350/383 of 2a9fA

query
sites
2a9fA

G

G

K

K

I

L

E

E

I

V

I

Q

A

P

S

K

K

V

P

D

M

I

A

K

T

T

Q

K

R

H

D

D

L

L

S

S

L

I

A

A

Y
|
Y

S

T

P

P

G

G

V

V

A

A

V

S

P

V

V

S

Q

S

A

A

I

I

A

A

D

K

D

D

P

K

A

T

T

L

A

A

Y

Y

D

D

Y

L

T

T

A

T

K

K

G

K

N

N

L

T

V

V

A

A

V

V

I

I

S

S

N

D

G

G

T

T

A
|
A

I

V

L

L

G

G

M

L

G

G

N

D

L

I

G

G

A

P

L

E

A

A

S

A

K

M

P

P

V

V

M

M

E

E

G

G

K
|
K

A

A

V

A

L

L

F

F

K

K

R

A

F

F

A

A

D

G

V

V

D

D

S

A

I

I

D

P

I

I

E

V

L

L

A

D

S

T

E

K

D

D

T

T

D

E

A

E

L

I

I

I

E

S

A

I

I

V

A

K

M

A

M

L

E

A

P

P

S

T

F

F

G

G

G

G

I

I

N

N

L

L

E
|
E

D
|
D

I

I

K

S

A

A

P

P

E

R

C

C

F

F

I

E

I

I

E

E

Q

Q

A

R

L

L

R

I

E

K

R

E

M

C

N

H

I

I

P

P

V

V

M

F

H

H

D
|
D

D
|
D

Q

Q

H

H

G

G

T

T

A

A

I

I

I

V

A

V

A

L

A

A

G

A

L

I

I

F

N

N

A

S

C

L

L

K

I

L

T

L

G

K

R

K

K

S

L

L

D

D

E

E

V

V

K

S

V

I

V

V

V

V

N

N

G

G

A

G

G

G

A

S

S

A

A

G

L

L

A

S

C

I

T

T

S

R

L

K

I

L

K

L

S

A

M

A

G

G

V

A

R

T

H

-

E

-

N

K

V

V

L

T

V

V

C

V

D

D

T

K

R

F

G

G

V

I

I

I

Y

-

P

-

G

N

R

E

E

Q

N

E

V

A

D

A

Q

Q

F

L

K

A

S

P

A

D

H

I

A

A

V

K

M

V

T

T

D

N

K

R

R

E

-

F

-

K

-

S

-

G

T

T

L

L

L

E

E

D

A

A

I

L

E

E

G

G

A

A

D

D

V

I

V

F

L

I

G

G

L

V

S

S

A

A

K

P

G

G

A

V

I

L

T

K

Q

A

D

E

M

W

V

I

K

S

R

K

M

M

A

A

P

A

E

R

P

P

I

V

I

I

F

F

A

A

M

M

A

A

N
|
N

P

P

D

I

P

P

E

E

I

I

T

Y

P

P

P

D

E

E

A

A

K

L

A

E

V

A

R

G

P

A

D

-

A

Y

I

I

V

V

A

G

T

T

G

G

R

R

S

S

D

D

Y

F

P

P

N

N

Q

Q

V

I

N

N

N

N

V

V

L

L

G

A

F

F

P

P

F

G

I

I

F

F

R

R

G

G

A

A

L

L

D

D

V

A

R

R

A

A

T

K

A

T

I

I

N

T

E

V

E

E

M

M

K

Q

I

I

A

A

A

A

A

A

T

K

A

G

I

I

A

A

E

S

L

L

active site: Y38 (= Y44), A74 (= A80), K93 (= K99), E135 (= E141), D136 (= D142), D160 (= D166), D161 (= D167), N285 (= N291)
binding magnesium ion: E135 (= E141), D136 (= D142), D161 (= D167)

2dvmA NAD complex structure of ph1275 protein from pyrococcus horikoshii
41% identity, 58% coverage: 13:453/754 of query aligns to 4:434/438 of 2dvmA

query
sites
2dvmA

E

K

A

A

L

L

F

E

Y

F

H

H

S

K

T

N

I

N

R

F

P

P

G

G

-

N

-

G

K

K

I

I

E

E

I

V

I

I

A

P

S

K

K

V

P

S

M

L

A

E

T

S

Q

R

R

E

D

E

L

L

S

T

L

L

A

A

Y
|
Y

S

T

P

P

G

G

V

V

A

A

V

E

P

P

V

C

Q

K

A

E

I

I

A

A

D

R

D

D

P

P

A

G

T

K

A

V

Y

Y

D

E

Y

Y

T

T

A

S

K

K

G

G

N

N

L

L

V

V

A

A

V

V

I

V

S

S

N

D

G

G

T

S

A
x
R

I

I

L

L

G

G

M

L

G

G

N

N

L

I

G

G

A

P

L

L

A

A

S

G

K

L

P

P

V

V

M

M

E

E

G

G

K
|
K

A

A

V

L

L

L

F

F

K

K

R

R

F

F

A

G

D

G

V

V

D

D

S

A

I

F

D

P

I

I

E

M

L

I

A

K

S

E

E

Q

D

E

T

P

D

N

A

K

L

F

I

I

E

D

A

I

I

V

A

K

M

A

M

I

E

A

P

P

S

T

F

F

G

G

G

G

I

I

N

N

L

L

E
|
E

D
|
D

I

I

K

A

A

S

P

P

E

K

C

C

F

F

I

Y

I

I

E

L

Q

E

A

R

L

L

R

R

E

E

R

E

M

L

N

D

I

I

P

P

V

V

M

F

H

H

D
|
D

D
|
D

Q

Q

H

Q

G

G

T
|
T

A

A

I

A

I

V

A

V

A

L

A

A

G

G

L

L

I

L

N

N

A

A

C

L

L

K

I

V

T

V

G

G

R

K

K

K

L

I

D

S

E

E

V

I

K

T

V

L

V

A

V

L

N

F

G

G

A

A

G
|
G

A
|
A

S
x
A

A

G

L

F

A

A

C

T

T

L

S

R

L

I

I

L

K

T

S

E

M

A

G

G

V

V

R

K

H

P

E

E

N

N

V

V

L

R

V

V

C
x
V

D
x
E

T
x
L

-

V

-

N

-

G

R

K

G
x
P

V

R

I

I

Y

L

P

T

G

S

R

D

E

L

N

D

V

L

D

E

Q

K

F

L

K

F

S

P

A

Y

H

R

A

G

V

W

M

L

T

L

D

-

K

K

R

K

T

T

L

N

L

G

E

E

A

N

I

I

E

E

G

G

-

G

-

P

-

Q

-

E

-

A

-

L

-

K

-

D

A

A

D

D

V

V

V

L

L

I

G

S

L
x
F

S
x
T

-

R

-

P

A

G

K

P

G

G

A

V

I

I

T

K

Q

P

D

Q

M

W

V

I

K

E

R

K

M

M

A

N

P

E

E

D

P

A

I

I

I

V

F

F

A

P

M
x
L

A

A

N
|
N

P

P

D

V

P

P

E

E

I

I

T

L

P

P

P

E

E

E

A

A

K

K

A

K

V

A

R

G

P

A

D

-

A

R

I

I

V

V

A

A

T

T

G

G

R

R

S

S

D

D

Y

Y

P

P

N

N

Q

Q

V

I

N

N

N
|
N

V

L

L

L

G

G

F

F

P

P

F

G

I

I

F

F

R

R

G

G

A

A

L

L

D

D

V

V

R

R

A

A

T

R

A

T

I

I

N

T

E

D

E

S

M

M

K

I

I

I

A

A

A

A

A

A

T

K

A

A

I

I

A

A

E

S

L

I

A

V

R

E

E

E

Q

P

V

S

H

E

E

E

D

N

V

-

A

-

A

-

A

-

Y

-

G

-

G

-

E

-

T

-

Q

-

V

-

F

-

G

-

R

-

D

-

Y

-

I

I

I

I

P

P

A

S

P

P

F

L

D

N

P

P

R

I

L

V

M

Y

E

A

V

R

V

E

P

A

M

R

A

A

V

V

A

A

K

E

A

E

A

A

M

M

E

K

S

E

G

G

V

V

A

A

Q

R

K

T

P

K

I

V

E

K

D

G

F

E

E

W

A

V

Y

E

R

E

H

H

Q

T

L

I

K

R

A

-

R

-

L

-

N

-

P

-

T

-

T

-

S

-

V

-

L

L

T

I

R

E

V

F

Y

Y

D

E

E

N

V

V

K

I

A

A

-

P

-

I

N

N

P

K

K

K

R

R

V

R

I

E

F

Y

A

S

E

K

A

A

active site: Y37 (= Y44), R73 (≠ A80), K92 (= K99), E134 (= E141), D135 (= D142), D159 (= D166), D160 (= D167), N296 (= N291)
binding nicotinamide-adenine-dinucleotide: T164 (= T171), G194 (= G201), A195 (= A202), A196 (≠ S203), V217 (≠ C224), E218 (≠ D225), L219 (≠ T226), P224 (≠ G228), F269 (≠ L266), T270 (≠ S267), L294 (≠ M289), N296 (= N291), N327 (= N323)

2haeD Crystal structure of a putative malic enzyme (malate oxidoreductase)
44% identity, 45% coverage: 13:355/754 of query aligns to 1:342/373 of 2haeD

query
sites
2haeD

E

D

A

A

L

L

F

E

Y

L

H

H

S

R

T

F

I

L

R

K

P

-

G

G

K

K

I

I

E

R

I

T

I

A

A

L

S

P

K

V

P

E

M

K

A

V

T

D

Q

R

R

E

D

T

L

L

S

S

L

L

A

L

Y
|
Y

S

T

P

P

G

G

V

V

A

A

V

D

P

V

V

A

Q

R

A

A

I

C

A

A

D

E

D

D

P

P

A

E

T

K

A

T

Y

Y

D

V

Y

Y

T

T

A

S

K

R

G

W

N

N

L

T

V

V

A

A

V

V

I

V

S

S

N

D

G

G

T

S

A
|
A

I

V

L

L

G

G

M

L

G

G

N

N

L

I

G

G

A

P

L

Y

A

G

S

A

K

L

P

P

V

V

M

M

E

E

G

G

K
|
K

A

A

V

F

L

L

F

F

K

K

R

A

F

F

A

A

D

D

V

I

D

D

S

A

I

F

D

P

I

I

E

C

L

L

A

S

S

E

E

S

D

E

T

E

D

E

A

K

L

I

I

I

E

S

A

I

I

V

A

K

M

S

M

L

E

E

P

P

S

S

F

F

G

G

G

G

I

I

N

N

L

L

E
|
E

D
|
D

I

I

K

G

A

A

P

P

E

K

C

C

F

F

I

R

I

I

E

L

Q

Q

A

R

L

L

R

S

E

E

R

E

M

M

N

N

I

I

P

P

V

V

M

F

H

H

D
|
D

D
|
D

Q

Q

H

Q

G

G

T

T

A

A

I

V

I

V

A

V

A

S

A

A

G

A

L

F

I

L

N

N

A

A

C

L

L

K

I

L

T

T

G

E

R

K

K

K

L

I

D

E

E

E

V

V

K

K

V

V

V

V

V

V

N

N

G

G

A

I

G

G

A

A

S

A

A

G

L

Y

A

N

C

I

T

V

S

K

L

F

I

L

K

L

S

D

M

L

G

G

V

V

R

K

H

-

E

-

N

N

V

V

L

V

V

A

C

V

D

D

T

R

R

K

G

G

V

I

I

L

Y

-

P

-

G

-

R

N

E

E

N

N

-

D

-

P

-

E

-

T

-

C

V

L

D

N

Q

E

F

Y

K

E

S

I

A

A

H

R

A

I

V

T

M

N

T

P

D

E

K

R

R

L

T

S

-

G

-

D

L

L

L

E

E

T

A

A

I

L

E

E

G

G

A

A

D

D

V

F

V

F

L

I

G

G

L

V

S

S

A

R

K

G

G

N

A

I

I

L

T

K

Q

P

D

E

M

W

V

I

K

K

R

K

M

M

A

S

P

R

E

K

P

P

I

V

I

I

F

F

A

A

M

L

A

A

N
|
N

P

P

D

V

P

P

E

E

I

I

T

D

P

P

P

E

E

L

A

A

K

R

A

E

V

A

R

G

P

A

D

-

A

F

I

I

V

V

A

A

T

T

G

G

R

R

S

S

D

D

Y

H

P

P

N

N

Q

Q

V

V

N

N

N

N

V

L

L

L

G

A

F

F

P

P

F

G

I

I

F

M

R

K

G

G

A

A

L

V

D

E

V

K

R

R

A

-

T

S

A

K

I

I

N

T

E

K

E

N

M

M

K

L

I

L

A

S

A

A

A

V

T

E

A

A

I

I

A

A

active site: Y31 (= Y44), A67 (= A80), K86 (= K99), E128 (= E141), D129 (= D142), D153 (= D166), D154 (= D167), N280 (= N291)
binding zinc ion: E128 (= E141), D154 (= D167)

2haeB Crystal structure of a putative malic enzyme (malate oxidoreductase)
44% identity, 45% coverage: 13:355/754 of query aligns to 1:342/373 of 2haeB

query
sites
2haeB

E

D

A

A

L

L

F

E

Y

L

H

H

S

R

T

F

I

L

R

K

P

-

G

G

K

K

I

I

E

R

I

T

I

A

A

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P

K

V

P

E

M

K

A

V

T

D

Q

R

R

E

D

T

L

L

S

S

L

L

A

L

Y
|
Y

S

T

P

P

G

G

V

V

A

A

V

D

P

V

V

A

Q

R

A

A

I

C

A

A

D

E

D

D

P

P

A

E

T

K

A

T

Y

Y

D

V

Y

Y

T

T

A

S

K

R

G

W

N

N

L

T

V

V

A

A

V

V

I

V

S

S

N

D

G

G

T

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A
|
A

I

V

L

L

G

G

M

L

G

G

N

N

L

I

G

G

A

P

L

Y

A

G

S

A

K

L

P

P

V

V

M

M

E

E

G

G

K
|
K

A

A

V

F

L

L

F

F

K

K

R

A

F

F

A

A

D

D

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I

D

D

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A

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D

P

I

I

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C

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L

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E

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E

D

E

A

K

L

I

I

I

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A

I

I

V

A

K

M

S

M

L

E

E

P

P

S

S

F

F

G

G

G

G

I

I

N

N

L

L

E
|
E

D
|
D

I

I

K

G

A

A

P

P

E

K

C

C

F

F

I

R

I

I

E

L

Q

Q

A

R

L

L

R

S

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E

R

E

M

M

N

N

I

I

P

P

V

V

M

F

H

H

D
|
D

D
|
D

Q

Q

H

Q

G

G

T
|
T

A

A

I

V

I

V

A

V

A

S

A

A

G

A

L

F

I

L

N

N

A

A

C

L

L

K

I

L

T

T

G

E

R

K

K

K

L

I

D

E

E

E

V

V

K

K

V

V

V

V

V

V

N
|
N

G

G

A

I

G
|
G

A
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A

S
x
A

A

G

L

Y

A

N

C

I

T

V

S

K

L

F

I

L

K

L

S

D

M

L

G

G

V

V

R

K

H

-

E

-

N

N

V

V

L

V

V

A

C

V

D
|
D

T
x
R

R

K

G

G

V

I

I

L

Y

-

P

-

G

-

R

N

E

E

N

N

-

D

-

P

-

E

-

T

-

C

V

L

D

N

Q

E

F

Y

K

E

S

I

A

A

H

R

A

I

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T

M

N

T

P

D

E

K

R

R

L

T

S

-

G

-

D

L

L

L

E

E

T

A

A

I

L

E

E

G

G

A

A

D

D

V

F

V

F

L

I

G

G

L
x
V

S
|
S

A
x
R

K

G

G

N

A

I

I

L

T

K

Q

P

D

E

M

W

V

I

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K

R

K

M

M

A

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R

E

K

P

P

I

V

I

I

F

F

A

A

M
x
L

A
|
A

N
|
N

P

P

D

V

P

P

E

E

I

I

T

D

P

P

P

E

E

L

A

A

K

R

A

E

V

A

R

G

P

A

D

-

A

F

I

I

V

V

A

A

T

T

G

G

R

R

S

S

D

D

Y

H

P

P

N

N

Q

Q

V

V

N

N

N
|
N

V

L

L

L

G

A

F

F

P

P

F

G

I

I

F

M

R

K

G

G

A

A

L

V

D

E

V

K

R

R

A

-

T

S

A

K

I

I

N

T

E

K

E

N

M

M

K

L

I

L

A

S

A

A

A

V

T

E

A

A

I

I

A

A

active site: Y31 (= Y44), A67 (= A80), K86 (= K99), E128 (= E141), D129 (= D142), D153 (= D166), D154 (= D167), N280 (= N291)
binding nicotinamide-adenine-dinucleotide: T158 (= T171), N185 (= N198), G188 (= G201), A189 (= A202), A190 (≠ S203), D210 (= D225), R211 (≠ T226), V255 (≠ L266), S256 (= S267), R257 (≠ A268), L278 (≠ M289), A279 (= A290), N280 (= N291), N311 (= N323)

1xcoD Crystal structure of a phosphotransacetylase from bacillus subtilis in complex with acetylphosphate (see paper)
27% identity, 42% coverage: 433:750/754 of query aligns to 6:322/325 of 1xcoD

query
sites
1xcoD

V

L

L

F

T

S

R

T

V

V

Y

Q

D

E

E

K

V

V

K

A

A

G

N

K

P

D

K

V

R

K

V

I

I

V

F

F

A

P

E

E

A

G

D

L

N

D

E

E

V

R

V

I

L

L

R

E

A

A

A

V

I

S

Q

K

Y

L

R

A

D

G

F

N

G

K

Y

V

G

L

E

N

P

P

V

I

L

V

V

I

G

G

R

N

T

E

Q

N

V

E

I

I

L

Q

D

A

R

K

M

A

A

K

E

E

L

L

G

N

-

L

-

T

-

L

-

G

-

G

-

V

-

K

V

I

P

Y

N

D

P

P

E

H

S

T

Y

Y

H

-

I

-

E

-

N

-

S

-

M

-

V

-

S

-

E

E

H

G

V

M

A

E

P

D

M

L

V

V

E

Q

M

A

L

F

Y

V

E

E

R
|
R

L

-

K

-

R
|
R

R

K

G

G

F
x
K

L

A

K

T

R

E

D

E

V

Q

Q

A

R

R

M

K

V

A

N

L

T

L

D

D

R

E

N

N

I

Y

F

F

A

G

S

T

G

M

L

L

L

V

K

Y

L

K

G

G

A

L

G

A

D

D

A

G

M

L

I

V

T

S

G

G

M

A

T

A

R

H

P

S

F
x
T

A

A

Q

D

S

T

M

V

K

R

E

P

V

A

R

L

R

Q

A

I

L

I

D
x
K

P

T

A

K

E

E

G

G

-

V

K

K

L

K

P

T

F

S

G

G

I

V

H

F

M

I

L

M

V

A

G

R

K

G

N

E

H

E

T

Q

V

Y

F

V

M

F

A

A

D

D

T

C

T

A

I

I

N

N

E

I

R

A

P

P

S

D

A

S

E

Q

E

D

L

L

A

A

V

E

I

I

A

A

T

I

E

E

T

S

A

A

A

N

V

T

A

A

R

K

K

M

L

F

G

D

H

I

E

E

P

P

R

R

V

V

A

A

F

M

L

L

S

S

Y

F

S

S

T

T

F

K

G

G

N

S

P

A

A

K

G

S

K

D

W

E

L

T

D

E

A

K

T

V

R

A

E

D

A

A

I

V

H

K

I

I

L

A

D

K

Q

E

Q

K

Q

A

P

P

D

E

F

L

E

T

Y

L

E

D

G

G

E

E

L

F

A

Q

P

F

D

D

A

A

A

A

L

F

N

V

P

P

K

S

V

V

M

A

A

E

L

K

Y

K

-

A

P

P

F

D

S

S

R

E

L

I

T

K

A

G

P

D

A

A

N

N

V

V

L

F

I

V

M

F

P

P

G

S

L

L

Q

E

S

A

A

G

N

N

I

I

S

G

A
x
Y

K
|
K

L

I

L

A

R

Q

E

R

I

L

G

G

G

N

T

F

T

E

S

A

I

V

G

G

P

P

M

I

L

L

L

Q

G

G

M

L

E
x
N

K

M

P

P

V

V

Q

N

I

D

V

L

P
x
S

M
x
R

T

G

A

C

L

N

A

A

P

E

D

D

V

V

L

Y

T

N

S

L

A

A

V

L

L

I

A

T

A

A

A

A

binding acetylphosphate: R90 (= R518), R91 (= R521), K94 (≠ F524), T131 (≠ F561), K143 (≠ D573), Y278 (≠ A706), K279 (= K707), N298 (≠ E726), S305 (≠ P733), R306 (≠ M734)

6ioxA Crystal structure of porphyromonas gingivalis phosphotransacetylase in complex with acetyl-coa (see paper)
29% identity, 40% coverage: 431:730/754 of query aligns to 5:312/339 of 6ioxA

query
sites
6ioxA

T

S

S

D

V

L

L

I

T

Q

R

D

V

V