SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_059153651.1 NCBI__GCF_001046635.1:WP_059153651.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q53TZ2 L-arabinose 1-dehydrogenase (NAD(P)(+)); D-galactose 1-dehydrogenase; EC 1.1.1.376; EC 1.1.1.120; EC 1.1.1.48 from Azospirillum brasilense (see paper)
55% identity, 99% coverage: 2:306/308 of query aligns to 3:307/309 of Q53TZ2

query
sites
Q53TZ2

D

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D169 (= D169) mutation to A: Loss of activity.
N173 (= N173) mutation to A: Decrease by 4 orders of magnitude in catalytic efficiency.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7cgqA Crystal structure of azospirillum brasilense l-arabinose 1- dehydrogenase e147a mutant (NADP and l-arabinose bound form)
55% identity, 98% coverage: 4:306/308 of query aligns to 2:304/306 of 7cgqA

query
sites
7cgqA

I

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binding alpha-L-arabinopyranose: K88 (= K91), H116 (= H119), H150 (= H153), P167 (= P170), N170 (= N173)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G12), K11 (= K13), I12 (= I14), S34 (= S36), R35 (≠ P37), H36 (= H38), C64 (= C67), A65 (≠ T68), P66 (= P69), V69 (= V72), E87 (= E90), K88 (= K91), H116 (= H119), Q153 (= Q156), W155 (= W158), D166 (= D169), Y263 (= Y265)

1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
26% identity, 62% coverage: 7:196/308 of query aligns to 35:241/381 of 1rydA

query
sites
1rydA

A

A

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active site: K129 (= K91), Y217 (≠ N173)
binding alpha-D-glucopyranose: Y236 (≠ A191)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L39 (≠ V11), G40 (= G12), K41 (= K13), Y42 (≠ I14), S64 (= S36), G65 (≠ P37), K69 (vs. gap), Y87 (vs. gap), L106 (≠ T68), P107 (= P69), N108 (≠ P70), L110 (≠ V72), H111 (≠ R73), E128 (= E90), K129 (= K91), R157 (≠ H119), A196 (≠ G155), W199 (= W158), R200 (≠ I159), Y217 (≠ N173)

Sites not aligning to the query:

1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
26% identity, 62% coverage: 7:196/308 of query aligns to 37:243/383 of 1h6dA

query
sites
1h6dA

A

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active site: K131 (= K91), Y219 (≠ N173)
binding glycerol: K131 (= K91), R202 (≠ I159), D215 (= D169), Y219 (≠ N173)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G40 (= G10), L41 (≠ V11), G42 (= G12), K43 (= K13), Y44 (≠ I14), S66 (= S36), G67 (≠ P37), K71 (vs. gap), Y89 (vs. gap), I107 (≠ C67), L108 (≠ T68), P109 (= P69), N110 (≠ P70), H113 (≠ R73), E130 (= E90), K131 (= K91), R159 (≠ H119), A198 (≠ G155), W201 (= W158), R202 (≠ I159), Y219 (≠ N173)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 17, 18, 19, 298

1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
25% identity, 69% coverage: 7:218/308 of query aligns to 6:237/340 of 1evjA

query
sites
1evjA

A

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K

A

V

A

G

N

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

C

R

H

F

Y

A

D

A

P

G

M

V

N

A

R

P

A

A

A

R

V

A

K

W

L

L

I

A

R

E

E

R

N

R

Q

I

L

E

G

S

K

V

L

K

G

I

M

V

V

W

T

R

T

E

D

D

N

V

S

R

D

V

V

W

M

-

D

-

Q

-

N

H

D

P

P

G

A

Q

Q

A

Q

W
|
W

I
x
R

W

L

E

R

-

R

-

E

-

L

P

A

G

G

L

G

G

S

V

L

F

M

D

D

P

I

G

G

I

I

N
x
Y

A

G

L

L

S

N

I

G

L

T

T

R

H

Y

I

L

L

L

-

G

P

E

R

E

P

P

V

I

F

E

L

V

K

R

T

A

A

Y

T

T

L

Y

E

S

I

D

P

P

A

N

N

D

-

E

R

R

A

F

A

V

P

E

I

V

A

E

A

D

D

R

I

I

-

I

-

W

Q

Q

F

M

R

R

D

F

T

R

A

S

G

G

A

A

P

L

I

S

H

H

active site: K100 (= K91), Y188 (≠ N173)
binding nicotinamide-adenine-dinucleotide: G9 (= G10), L10 (≠ V11), G11 (= G12), K12 (= K13), Y13 (≠ I14), D35 (≠ S36), L77 (≠ T68), P78 (= P69), N79 (≠ P70), H82 (≠ R73), E99 (= E90), K100 (= K91), R128 (≠ H119), W170 (= W158), R171 (≠ I159), Y188 (≠ N173)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 267

3m2tA The crystal structure of dehydrogenase from chromobacterium violaceum
28% identity, 41% coverage: 4:130/308 of query aligns to 4:136/342 of 3m2tA

query
sites
3m2tA

I

I

R

K

I

V

A

G

V

L

V

V

G
|
G

V

I

G
|
G

K
x
A

I
x
Q

A

M

R

Q

D

E

Q

N

H

L

L

L

P

P

A

S

I

L

A

L

G

Q

N

M

K

Q

A

D

F

I

S

R

L

I

A

V

A

A

T

A

V

C

S
x
D

P
x
S

H

D

D

L

A

E

G
x
R

V

A

E

R

G

R

V

V

S

H

H

R

H

F

K

I

S

S

L

D

D

I

E

P

L

V

I

L

E

D

N

N

G

V

P

P

A

A

V

M

-

L

-

N

-

Q

-

V

-

P

-

L

D

D

A

A

V

V

A

V

L

M

C
x
A

T
x
G

P

P

Q

Q

V
x
L

R

H

Y

F

D

E

L

M

A

G

V

L

Q

L

A

A

L

M

N

S

K

K

G

G

M

V

H

N

V

V

F

F

L

V

E
|
E

K
|
K

P

P

G

C

A

A

T

T

L

L

S

E

E

E

C

L

V

E

A

T

L

L

S

I

S

D

R

A

A

A

E

R

K

R

V

S

G

D

T

V

L

S

F

G

A

V

A

G

W

M

H
x
N

S

F

R

K

F

F

A

A

A

R

G

P

V

V

A

R

P

Q

A

L

R

R

binding nicotinamide-adenine-dinucleotide: G10 (= G10), G12 (= G12), A13 (≠ K13), Q14 (≠ I14), D36 (≠ S36), S37 (≠ P37), R41 (≠ G41), A73 (≠ C67), G74 (≠ T68), L78 (≠ V72), E96 (= E90), K97 (= K91), N125 (≠ H119)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 290

1zh8A Crystal structure of oxidoreductase (tm0312) from thermotoga maritima at 2.50 a resolution
33% identity, 45% coverage: 1:140/308 of query aligns to 1:142/325 of 1zh8A

query
sites
1zh8A

M

L

D

R

P

K

I

I

R

R

I

L

A

G

V

I

V

V

G
|
G

V
x
C

G
|
G

K
x
I

I
x
A

A

A

R

R

D

E

Q

L

H

H

L

L

P

P

A

A

I

L

A

K

G

N

-

L

N

S

K

H

A

L

F

F

S

E

L

I

A

T

A

A

T

V

V

T

S
|
S

P
x
R

H
x
T

D

R

A

S

G
x
H

V

A

E

E

G

E

V

F

S

A

H

K

-

M

-

V

-

G

-

N

-

P

-

A

-

V

H

F

K

D

S

S

L

Y

D

E

E

E

L

L

I

L

E

E

N

S

G

G

P

-

A

L

V

V

D

D

A

A

V

V

A

D

L

L

C
x
T

T
x
L

P
|
P

P

V

Q

E

V
x
L

R

N

Y

L

D

P

L

F

A

I

V

E

Q

K

A

A

L

L

N

R

K

K

G

G

M

V

H

H

V

V

F

I

L

C

E
|
E

K
|
K

P

P

P

I

G

S

A

T

-

D

-

V

-

E

T

T

L

G

S

K

E

K

C

V

V

V

A

E

L

L

S

S

S

E

R

K

A

S

E

E

K

K

V

-

G

-

T

-

T

T

L

V

F

Y

A

I

A

A

W

E

H
x
N

S

F

R

R

F

H

A

-

G

-

V

-

A

V

P

P

A

A

R

-

A

F

W

W

L

K

A

A

E

K

R

E

R

L

I

V

E

E

S

S

active site: K98 (= K91)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), C11 (≠ V11), G12 (= G12), I13 (≠ K13), A14 (≠ I14), S37 (= S36), R38 (≠ P37), T39 (≠ H38), H42 (≠ G41), T74 (≠ C67), L75 (≠ T68), P76 (= P69), L79 (≠ V72), E97 (= E90), K98 (= K91), N126 (≠ H119)

Sites not aligning to the query:

active site: 187
binding nadp nicotinamide-adenine-dinucleotide phosphate: 165, 170, 171, 187, 276

5a05A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with maltotriose (see paper)
27% identity, 57% coverage: 7:181/308 of query aligns to 6:193/335 of 5a05A

query
sites
5a05A

A

A

V

I

V

L

G
|
G

V
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

L

M

P

P

A

R

I

F

A

A

G

E

N

C

K

E

A

H

F

S

S

R

L

L

A

A

T

L

V

V

S
|
S

-
x
G

-
x
T

P

P

H

E

D
x
K

A

L

G

K

V

T

E

Y

G

G

V

E

S

Q

H

Y

-

G

-

I

-

P

-

E

-

T

-

H

-

R

-

Y

-

S

H
x
Y

K

E

S

T

L

F

D

D

E

R

L

I

I

I

E

D

N

N

G

-

P

P

A

D

V

V

D

D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P
x
N

Q

S

V
x
L

R
x
H

Y

R

D

P

L

F

A

T

V

E

Q

R

A

A

L

A

N

R

K

A

G

G

M

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

A

N

T

T

L

V

S

A

E

D

C

C

V

E

A

A

L

M

S

I

S

A

R

A

A

C

E

K

K

K

V

A

G

G

T

R

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

G

H

V

N

A

I

P

E

A

A

R

I

A

K

W

L

L

V

A

R

E

D

R

G

R

A

I

L

E

G

S

P

V

V

K

R

I

T

V

V

W

V

R

T

E

D

-

H

-

G

-
x
F

-

T

-

I

-

G

D

D

V

P

R

K

V

Q

W
|
W

H
x
R

P

L

G

N

Q

R

A

A

W

L

I

A

W

-

E

-

P

-

G

G

L

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

I

A

L

A

T

R

H

Y

I

L

active site: K100 (= K91), Y185 (≠ N173)
binding beta-D-glucopyranose: K100 (= K91), F159 (vs. gap), D181 (= D169), Y185 (≠ N173)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), L10 (≠ V11), G11 (= G12), Y12 (≠ K13), Y13 (≠ I14), S35 (= S36), G36 (vs. gap), T37 (vs. gap), K40 (≠ D39), Y58 (≠ H48), I76 (≠ C67), T77 (= T68), P78 (= P69), N79 (≠ P70), L81 (≠ V72), H82 (≠ R73), E99 (= E90), K100 (= K91), R128 (≠ H119), W167 (= W152), R168 (≠ H153), Y185 (≠ N173)

Sites not aligning to the query:

5a04A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glucose (see paper)
27% identity, 57% coverage: 7:181/308 of query aligns to 6:193/335 of 5a04A

query
sites
5a04A

A

A

V

I

V

L

G

G

V
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

L

M

P

P

A

R

I

F

A

A

G

E

N

C

K

E

A

H

F

S

S

R

L

L

A

A

T

L

V

V

S
|
S

-
x
G

-
x
T

P

P

H

E

D
x
K

A

L

G

K

V

T

E

Y

G

G

V

E

S

Q

H

Y

-

G

-

I

-

P

-

E

-

T

-

H

-

R

-

Y

-

S

H
x
Y

K

E

S

T

L

F

D

D

E

R

L

I

I

I

E

D

N

N

G

-

P

P

A

D

V

V

D

D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P
x
N

Q

S

V
x
L

R
x
H

Y

R

D

P

L

F

A

T

V

E

Q

R

A

A

L

A

N

R

K

A

G

G

M

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

A

N

T

T

L

V

S

A

E

D

C

C

V

E

A

A

L

M

S

I

S

A

R

A

A

C

E

K

K

K

V

A

G

G

T

R

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

G

H

V

N

A

I

P

E

A

A

R

I

A

K

W

L

L

V

A

R

E

D

R

G

R

A

I

L

E

G

S

P

V

V

K

R

I

T

V

V

W

V

R

T

E

D

-

H

-

G

-
x
F

-

T

-

I

-

G

D

D

V

P

R

K

V

Q

W
|
W

H
x
R

P

L

G

N

Q

R

A

A

W

L

I

A

W

-

E

-

P

-

G

G

L

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

I

A

L

A

T

R

H

Y

I

L

active site: K100 (= K91), Y185 (≠ N173)
binding beta-D-glucopyranose: K100 (= K91), F159 (vs. gap), R168 (≠ H153), D181 (= D169), Y185 (≠ N173)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L10 (≠ V11), G11 (= G12), Y12 (≠ K13), Y13 (≠ I14), S35 (= S36), G36 (vs. gap), T37 (vs. gap), K40 (≠ D39), Y58 (≠ H48), I76 (≠ C67), T77 (= T68), P78 (= P69), N79 (≠ P70), L81 (≠ V72), H82 (≠ R73), E99 (= E90), K100 (= K91), R128 (≠ H119), W167 (= W152), R168 (≠ H153), Y185 (≠ N173)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 263

5a03E Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
27% identity, 57% coverage: 7:181/308 of query aligns to 6:193/335 of 5a03E

query
sites
5a03E

A

A

V

I

V

L

G
|
G

V
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

L

M

P

P

A

R

I

F

A

A

G

E

N

C

K

E

A

H

F

S

S

R

L

L

A

A

T

L

V

V

S
|
S

-
x
G

-
x
T

P

P

H

E

D
x
K

A

L

G

K

V

T

E

Y

G

G

V

E

S

Q

H

Y

-

G

-

I

-

P

-

E

-

T

-

H

-

R

-

Y

-

S

H
x
Y

K

E

S

T

L

F

D

D

E

R

L

I

I

I

E

D

N

N

G

-

P

P

A

D

V

V

D

D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P
x
N

Q

S

V

L

R
x
H

Y

R

D

P

L

F

A

T

V

E

Q

R

A

A

L

A

N

R

K

A

G

G

M

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

A

N

T

T

L

V

S

A

E

D

C

C

V

E

A

A

L

M

S

I

S

A

R

A

A

C

E

K

K

K

V

A

G

G

T

R

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

G
x
H

V

N

A

I

P

E

A

A

R

I

A

K

W

L

L

V

A

R

E

D

R

G

R

A

I

L

E

G

S

P

V

V

K

R

I

T

V

V

W

V

R

T

E

D

-

H

-

G

-
x
F

-

T

-

I

-

G

D

D

V

P

R

K

V

Q

W
|
W

H
x
R

P

L

G

N

Q

R

A

A

W

L

I

A

W

-

E

-

P

-

G

G

L

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

I

A

L

A

T

R

H

Y

I

L

active site: K100 (= K91), Y185 (≠ N173)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), L10 (≠ V11), G11 (= G12), Y12 (≠ K13), Y13 (≠ I14), S35 (= S36), G36 (vs. gap), T37 (vs. gap), K40 (≠ D39), Y58 (≠ H48), I76 (≠ C67), T77 (= T68), P78 (= P69), N79 (≠ P70), H82 (≠ R73), E99 (= E90), K100 (= K91), R128 (≠ H119), W167 (= W152), R168 (≠ H153), Y185 (≠ N173)
binding beta-D-xylopyranose: K100 (= K91), F159 (vs. gap), R168 (≠ H153), D181 (= D169), Y185 (≠ N173)
binding alpha-D-xylopyranose: H134 (≠ G125)

Sites not aligning to the query:

5a02A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glycerol (see paper)
27% identity, 57% coverage: 7:181/308 of query aligns to 6:193/335 of 5a02A

query
sites
5a02A

A

A

V

I

V

L

G

G

V
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

L

M

P

P

A

R

I

F

A

A

G

E

N

C

K

E

A

H

F

S

S

R

L

L

A

A

T

L

V

V

S
|
S

-
x
G

-
x
T

P

P

H

E

D
x
K

A

L

G

K

V

T

E

Y

G

G

V

E

S

Q

H

Y

-

G

-

I

-

P

-

E

-

T

-

H

-

R

-

Y

-

S

H
x
Y

K

E

S

T

L

F

D

D

E

R

L

I

I

I

E

D

N

N

G

-

P

P

A

D

V

V

D

D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P

N

Q

S

V
x
L

R
x
H

Y

R

D

P

L

F

A

T

V

E

Q

R

A

A

L

A

N

R

K

A

G

G

M

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

A

N

T

T

L

V

S

A

E

D

C

C

V

E

A

A

L

M

S

I

S

A

R

A

A

C

E

K

K

K

V

A

G

G

T

R

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

G

H

V

N

A

I

P

E

A

A

R

I

A

K

W

L

L

V

A

R

E

D

R

G

R

A

I

L

E

G

S

P

V

V

K

R

I

T

V

V

W

V

R

T

E

D

-

H

-

G

-

F

-

T

-

I

-

G

D

D

V

P

R

K

V

Q

W
|
W

H
x
R

P

L

G

N

Q

R

A

A

W

L

I

A

W

-

E

-

P

-

G

G

L

G

G

S

V

L

F

M

D

D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

I

A

L

A

T

R

H

Y

I

L

active site: K100 (= K91), Y185 (≠ N173)
binding nadp nicotinamide-adenine-dinucleotide phosphate: L10 (≠ V11), G11 (= G12), Y12 (≠ K13), Y13 (≠ I14), S35 (= S36), G36 (vs. gap), T37 (vs. gap), K40 (≠ D39), Y58 (≠ H48), I76 (≠ C67), T77 (= T68), P78 (= P69), L81 (≠ V72), H82 (≠ R73), E99 (= E90), K100 (= K91), R128 (≠ H119), W167 (= W152), R168 (≠ H153), Y185 (≠ N173)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 263

5a06A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with sorbitol (see paper)
27% identity, 57% coverage: 7:181/308 of query aligns to 7:194/336 of 5a06A

query
sites
5a06A

A

A

V

I

V

L

G

G

V
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

L

M

P

P

A

R

I

F

A

A

G

E

N

C

K

E

A

H

F

S

S

R

L

L

A

A

T

L

V

V

S
|
S

-
x
G

-
x
T

P

P

H

E

D
x
K

A

L

G

K

V

T

E

Y

G

G

V

E

S

Q

H

Y

-

G

-

I

-

P

-

E

-

T

-

H

-

R

-

Y

-

S

H
x
Y

K

E

S

T

L

F

D

D

E

R

L

I

I

I

E

D

N

N

G

-

P

P

A

D

V

V

D
|
D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P
x
N

Q

S

V
x
L

R
x
H

Y

R

D

P

L

F

A

T

V

E

Q

R

A

A

L

A

N

R

K

A

G

G

M

K

H
|
H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

A

N

T

T

L

V

S

A

E

D

C

C

V

E

A

A

L

M

S

I

S

A

R

A

A

C

E

K

K

K

V

A

G

G

T
x
R

T

K

L
|
L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

G

H

V

N

A

I

P

E

A

A

R

I

A

K

W

L

L

V

A

R

E

D

R

G

R

A

I

L

E

G

S

P

V

V

K

R

I

T

V

V

W

V

R

T

E

D

-

H

-

G

-
x
F

-

T

-

I

-

G

D

D

V

P

R

K

V

Q

W
|
W

H
x
R

P

L

G

N

Q

R

A

A

W

L

I

A

W

-

E

-

P

-

G

G

L

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

I

A

L

A

T

R

H

Y

I

L

active site: K101 (= K91), Y186 (≠ N173)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L11 (≠ V11), G12 (= G12), Y13 (≠ K13), Y14 (≠ I14), S36 (= S36), G37 (vs. gap), T38 (vs. gap), K41 (≠ D39), Y59 (≠ H48), I77 (≠ C67), T78 (= T68), P79 (= P69), N80 (≠ P70), L82 (≠ V72), H83 (≠ R73), E100 (= E90), K101 (= K91), R129 (≠ H119), W168 (= W152), R169 (≠ H153), Y186 (≠ N173)
binding sorbitol: D72 (= D62), H96 (= H86), K101 (= K91), R122 (≠ T112), R122 (≠ T112), L124 (= L114), F160 (vs. gap), R169 (≠ H153), D182 (= D169), Y186 (≠ N173)

Sites not aligning to the query:

5a03C Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
27% identity, 57% coverage: 7:181/308 of query aligns to 7:194/336 of 5a03C

query
sites
5a03C

A

A

V

I

V

L

G
|
G

V
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

L

M

P

P

A

R

I

F

A

A

G

E

N

C

K

E

A

H

F

S

S

R

L

L

A

A

T

L

V

V

S
|
S

-
x
G

-
x
T

P

P

H

E

D
x
K

A

L

G

K

V

T

E

Y

G

G

V

E

S

Q

H

Y

-

G

-

I

-

P

-

E

-

T

-

H

-

R

-

Y

-

S

H
x
Y

K

E

S

T

L

F

D

D

E

R

L

I

I

I

E

D

N

N

G

-

P

P

A

D

V

V

D

D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P
x
N

Q

S

V
x
L

R
x
H

Y

R

D

P

L

F

A

T

V

E

Q

R

A

A

L

A

N

R

K

A

G

G

M

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

A

N

T

T

L

V

S

A

E

D

C

C

V

E

A

A

L

M

S

I

S

A

R

A

A

C

E

K

K

K

V

A

G

G

T

R

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

G

H

V

N

A

I

P

E

A

A

R

I

A

K

W

L

L

V

A

R

E

D

R

G

R

A

I

L

E

G

S

P

V

V

K

R

I

T

V

V

W

V

R

T

E

D

-

H

-

G

-
x
F

-

T

-

I

-

G

D

D

V

P

R

K

V

Q

W
|
W

H
x
R

P

L

G

N

Q

R

A

A

W

L

I

A

W

-

E

-

P

-

G

G

L

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

I

A

L

A

T

R

H

Y

I

L

active site: K101 (= K91), Y186 (≠ N173)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), L11 (≠ V11), G12 (= G12), Y13 (≠ K13), Y14 (≠ I14), S36 (= S36), G37 (vs. gap), T38 (vs. gap), K41 (≠ D39), Y59 (≠ H48), I77 (≠ C67), T78 (= T68), P79 (= P69), N80 (≠ P70), L82 (≠ V72), H83 (≠ R73), E100 (= E90), K101 (= K91), R129 (≠ H119), W168 (= W152), R169 (≠ H153), Y186 (≠ N173)
binding beta-D-xylopyranose: K101 (= K91), F160 (vs. gap), R169 (≠ H153), D182 (= D169), Y186 (≠ N173)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 264

9azoA Crystal structure of chms dehydrogenase pmdc from comamonas testosteroni bound to cofactor NADP (see paper)
24% identity, 55% coverage: 59:228/308 of query aligns to 63:235/315 of 9azoA

query
sites
9azoA

P

P

A

E

V

V

D

D

A

A

V

V

A

I

L

L

C
|
C

T
|
T

P

P

P
x
T

Q

Q

V

M

R
x
H

Y

A

D

E

L

Q

A

A

V

I

Q

A

A

C

L

M

N

K

K

A

G

G

M

K

H

H

V

V

F

Q

L

V

E
|
E

K
x
I

P

P

P

L

G

A

A

D

T

A

L

L

S

K

E

D

C

A

V

Q

A

E

L

V

S

A

S

E

R

L

A

Q

E

K

K

Q

V

T

G

G

T

L

T

V

L

A

F

M

A

V

A

G

W

H

H

T

S

R

R

R

F

F

A

-

G

-

V

-

A

N

P

P

A

S

R

H

A

Q

W

W

L

V

A

-

E

H

R

K

I

I

E

E

S

A

V

G

K

E

I

F

-

N

V

I

W

Q

R

Q

E

M

D

D

V

V

R

Q

V

T

W

Y

H

F

-

F

-

R

-

T

-

N

-

M

-
x
N

-

A

P

L

G

G

Q

Q

A

A

W
x
R

I

S

W
|
W

E

T

P

D

G

H

G

L

L

L

G

-

V

-

F

W

D
x
H

P

H

G

A

I

A

N
x
H

A

T

L

V

S

D

I

L

L

F

T

A

H

Y

I

Q

L

A

P

G

R

S

P

P

V

I

F

-

L

V

K

K

T

A

A

N

T

A

L

V

E

Q

I

G

P

P

A

I

N

H

R

K

A

D

A

L

P

G

I

I

A

A

A

M

D

D

-

M

-

S

I

I

Q

Q

F

L

R

K

D

A

T

A

A

N

G

G

A

A

P

I

I

C

H

T

M

L

D

S

L

L

D

S

W

F

R

N

Q

N

T

D

G

G

P

P

binding nadp nicotinamide-adenine-dinucleotide phosphate: C71 (= C67), T72 (= T68), T74 (≠ P70), H77 (≠ R73), E94 (= E90), I95 (≠ K91), N160 (vs. gap), R166 (≠ W158), W168 (= W160), H175 (≠ D169), H179 (≠ N173)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 9, 11, 12, 13, 34, 35

6o15A Crystal structure of a putative oxidoreductase yjhc from escherichia coli in complex with NAD(h) (see paper)
31% identity, 28% coverage: 50:135/308 of query aligns to 51:135/355 of 6o15A

query
sites
6o15A

S

S

L

L

D

D

E

A

L

L

I

V

E

-

N

S

G

S

P

K

A

L

V

V

D

D

A

C

V

V

A

I

L

V

C
x
A

T
|
T

P
|
P

P
x
N

Q

Y

V

L

R
x
H

Y

K

D

E

L

P

A

V

V

I

Q

K

A

A

L

A

N

K

K

N

G

K

M

K

H

H

V

V

F

F

L

C

E
|
E

K
|
K

P

P

P

I

G

A

A

L

T

S

L

Y

S

E

E

D

C

C

V

V

A

D

L

M

S

V

S

K

R

A

A

C

E

K

K

E

V

A

G

G

T

V

T

T

L

F

F

M

A

A

A

G

W

H

H
x
I

S

M

R

N

F

F

A

F

A

N

G

G

V

V

A

Q

P

Y

A

A

R

R

A

K

W

L

L

I

A

K

E

E

binding nicotinamide-adenine-dinucleotide: A67 (≠ C67), T68 (= T68), P69 (= P69), N70 (≠ P70), H73 (≠ R73), E90 (= E90), K91 (= K91), I119 (≠ H119)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 11, 12, 32, 33, 160

P39353 2,7-anhydro-N-acetylneuraminate hydratase; EcNanOx; EC 4.2.1.- from Escherichia coli (strain K12) (see paper)
31% identity, 28% coverage: 50:135/308 of query aligns to 51:135/372 of P39353

query
sites
P39353

S

S

L

L

D

D

E

A

L

L

I

V

E

-

N

S

G

S

P

K

A

L

V

V

D

D

A

C

V

V

A

I

L

V

C

A

T
|
T

P

P

P
x
N

Q

Y

V

L

R
x
H

Y

K

D

E

L

P

A

V

V

I

Q

K

A

A

L

A

N

K

K

N

G

K

M

K

H

H

V

V

F

F

L

C

E
|
E

K
|
K

P

P

P

I

G

A

A

L

T

S

L

Y

S

E

E

D

C

C

V

V

A

D

L

M

S

V

S

K

R

A

A

C

E

K

K

E

V

A

G

G

T

V

T

T

L

F

F

M

A

A

A

G

W

H

H

I

S

M

R

N

F

F

A

F

A

N

G

G

V

V

A

Q

P

Y

A

A

R

R

A

K

W

L

L

I

A

K

E

E

T68 (= T68) binding NAD(+)
N70 (≠ P70) binding NAD(+)
H73 (≠ R73) binding NAD(+)
E90 (= E90) binding NAD(+)
K91 (= K91) binding NAD(+)

Sites not aligning to the query:

11 binding NAD(+)
12 binding NAD(+)
33 binding NAD(+)
36 binding NAD(+)
160 binding NAD(+)

7xr9A Crystal structure of dgpa with glucose (see paper)
28% identity, 40% coverage: 1:122/308 of query aligns to 2:122/344 of 7xr9A

query
sites
7xr9A

M

M

D

S

P

K

I

L

R

K

I

I

A

G

V

I

G

G

V

C

G
|
G

K
x
G

I
|
I

A

A

R

N

D

Q

Q

K

H

H

L

F

P

P

A

A

I

L

A

K

G

N

N

N

K

A

A

D

F

L

S

N

-

E

L

I

A

V

A

A

T

E

V

K

S

A

P

A

H

A

D

E

A

F

G

G

V

-

E

E

G

G

V

A

S

Q

H

V

H

T

K

A

S
x
D

L
x
Y

D

K

E

E

L

L

I

L

E

A

N

N

G

-

P

P

A

E

V

V

D

E

A

V

V

V

A

H

L

V

C
|
C

T
|
T

P
|
P

P

N

Q

V

V

S

R
x
H

Y

S

D

E

L

I

A

T

V

I

Q

A

A

A

L

F

N

E

K

A

G

G

M

K

H

H

V

V

F

Y

L

C

E
|
E

K
|
K

P
|
P

P

M

G

S

A

H

T

S

L

T

S

E

E

E

C

A

V

E

A

K

L

M

S

V

S

E

R

A

A

W

E

K

K

K

V

S

G

G

T

K

T

Q

L

F

F

T

A

I

A

G

W

Y

H
x
Q

S

N

R

R

F

F

binding beta-D-glucopyranose: K91 (= K91)
binding nicotinamide-adenine-dinucleotide: G13 (= G12), G14 (≠ K13), I15 (= I14), D51 (≠ S50), Y52 (≠ L51), C67 (= C67), T68 (= T68), P69 (= P69), H73 (≠ R73), E90 (= E90), K91 (= K91), P92 (= P92), Q119 (≠ H119)

Sites not aligning to the query:

2glxA Crystal structure analysis of bacterial 1,5-af reductase (see paper)
30% identity, 44% coverage: 5:138/308 of query aligns to 2:140/332 of 2glxA

query
sites
2glxA

R

R

I

W

A

G

V

L

V

I

G
|
G

V
x
A

G
x
S

K
x
T

I
|
I

A

A

R

R

D

E

Q

W

H

V

L

I

P

G

A

A

I

I

-

R

-

A

A

T

G

G

N

G

K

E

A

V

F

V

S

S

L

M

A

M

A
x
S

T
|
T

V

S

S

A

P

E

H
x
R

D

G

A

A

G

A

V

Y

-

A

-

T

-

E

E

N

G

G

V

I

S

G

H

K

H

S

-

V

K

T

S

S

L

V

D

E

E

E

L

L

I

V

E

G

N

D

G

-

P

P

A

D

V

V

D

D

A

A

V

V

A

Y

L

V

C
x
S

T
|
T

P

T

P
x
N

Q

E

V

L

R
x
H

Y

R

D

E

L

Q

A

T

V

L

Q

A

A

A

L

I

N

R

K

A

G

G

M

K

H

H

V

V

F

L

L

C

E
|
E

K
|
K

P

P

P

L

G

A

A

M

T

T

L

L

S

E

E

D

C

A

V

R

A

E

L

M

S

V

R

A

A

A

E

R

K

E

V

A

G

G

T

V

L

L

F

G

A

T

A

N

W

H

H
|
H

S

L

R

R

F

N

A

A

G

A

V

H

A

R

P

A

A

M

R

R

A

D

W

A

L

I

A

A

E

E

R

G

R

R

I

I

active site: K93 (= K91)
binding acetate ion: K93 (= K91)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G10), A8 (≠ V11), S9 (≠ G12), T10 (≠ K13), I11 (= I14), S32 (≠ A33), T33 (= T34), R37 (≠ H38), S69 (≠ C67), T70 (= T68), N72 (≠ P70), H75 (≠ R73), E92 (= E90), K93 (= K91), H121 (= H119)

Sites not aligning to the query:

active site: 179
binding acetate ion: 179
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 161, 162, 282

Q2I8V6 1,5-anhydro-D-fructose reductase; Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming); EC 1.1.1.292 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
30% identity, 44% coverage: 5:138/308 of query aligns to 3:141/333 of Q2I8V6

query
sites
Q2I8V6

R

R

I

W

A

G

V

L

V

I

G

G

V
x
A

G
x
S

K
x
T

I
|
I

A
|
A

R

R

D

E

Q

W

H

V

L

I

P

G

A

A

I

I

-

R

-

A

A

T

G

G

N

G

K

E

A

V

F

V

S

S

L

M

A

M

A
x
S

T
|
T

V

S

S

A

P

E

H
x
R

D

G

A

A

G

A

V

Y

-

A

-

T

-

E

E

N

G

G

V

I

S

G

H

K

H

S

-

V

K

T

S

S

L

V

D

E

E

E

L

L

I

V

E

G

N

D

G

-

P

P

A

D

V

V

D

D

A

A

V

V

A

Y

L

V

C

S

T
|
T

P
x
T

P
x
N

Q
x
E

V
x
L

R
x
H

Y

R

D

E

L

Q

A

T

V

L

Q

A

A

A

L

I

N

R

K

A

G

G

M

K

H

H

V

V

F

L

L

C

E
|
E

K
|
K

P

P

P

L

G

A

A

M

T

T

L

L

S

E

E

D

C

A

V

R

A

E

L

M

S

V

R

A

A

A

E

R

K

E

V

A

G

G

T

V

L

L

F

G

A

T

A
x
N

W

H

H

H

S

L

R

R

F

N

A

A

G

A

V

H

A

R

P

A

A

M

R

R

A

D

W

A

L

I

A

A

E

E

R

G

R

R

I

I

ASTI 9:12 (≠ VGKI 11:14) binding NADP(+)
S10 (≠ G12) mutation to G: Almost no effect.
A13 (= A15) mutation to G: Can use NAD as cosubstrate as well as NADP.
S33 (≠ A33) mutation to D: No activity.
ST 33:34 (≠ AT 33:34) binding NADP(+)
R38 (≠ H38) binding NADP(+)
TTNELH 71:76 (≠ TPPQVR 68:73) binding NADP(+)
EK 93:94 (= EK 90:91) binding NADP(+)
K94 (= K91) mutation to G: Less than 1% remaining activity.
N120 (≠ A117) binding NADP(+)

Sites not aligning to the query:

162:163 binding NADP(+)
176 D→A: Less than 1% remaining activity.
180 H→A: Less than 2% remaining activity.
206 G→I: No effect.
283 binding NADP(+)

4koaA Crystal structure analysis of 1,5-anhydro-d-fructose reductase from sinorhizobium meliloti (see paper)
30% identity, 44% coverage: 4:138/308 of query aligns to 2:141/333 of 4koaA

query
sites
4koaA

I

I

R

R

I

W

A

G

V

L

V

I

G
|
G

V
x
A

G
x
S

K
x
T

I
|
I

A

A

R

R

D

E

Q

W

H

V

L

I

P

G

A

A

I

I

-

R

-

A

A

A

G

G

N

G

K

E

A

V

F

V

S

S

L

V

A

M

A
x
S

T
x
S

V

S

S

A

P

E

H
x
R

D

G

-

E

-

A

-

Y

A

A

G

A

V

E

E

N

G

G

V

I

S

A

H

K

H

A

-

V

K

T

S

S

L

V

D

D

E

D

L

L

I

V

E

G

N

D

G

-

P

P

A

D

V

V

D

D

A

A

V

V

A

Y

L

I

C

S

T
|
T

P

T

P
x
N

Q

E

V

L

R
x
H

Y

H

D

G

L

Q

A

A

V

L

Q

A

A

A

L

I

N

R

K

A

G

G

M

K

H

H

V

V

F

L

L

C

E

E

K
|
K

P

P

P

L

G

A

A

M

T

N

L

L

S

N

E

D

C

G

V

C

A

E

L

M

S

V

S

L

R

K

A

A

E

C

K

E

V

A

G

G

T

V

L

L

F

G

A

T

A

N

W

H

H

H

S

L

R

R

F

N

A

A

G

T

V

H

A

R

P

A

A

M

R

R

A

E

W

A

L

I

A

A

E

A

R

G

R

R

I

I

active site: K94 (= K91)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), A9 (≠ V11), S10 (≠ G12), T11 (≠ K13), I12 (= I14), S33 (≠ A33), S34 (≠ T34), R38 (≠ H38), T71 (= T68), N73 (≠ P70), H76 (≠ R73), K94 (= K91)

Sites not aligning to the query:

active site: 180
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 160

Query Sequence

>WP_059153651.1 NCBI__GCF_001046635.1:WP_059153651.1
MDPIRIAVVGVGKIARDQHLPAIAGNKAFSLAATVSPHDAGVEGVSHHKSLDELIENGPA
VDAVALCTPPQVRYDLAVQALNKGMHVFLEKPPGATLSECVALSSRAEKVGTTLFAAWHS
RFAAGVAPARAWLAERRIESVKIVWREDVRVWHPGQAWIWEPGGLGVFDPGINALSILTH
ILPRPVFLKTATLEIPANRAAPIAADIQFRDTAGAPIHMDLDWRQTGPQSWDIVVETDAG
TLKLSNGGAVLTLPTGAEHSEDLEYPGLYARFANLIRGGRSDVDIAPLRLVADAFLRGNR
QTVDAFHD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory