SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_059175859.1 NCBI__GCF_001514535.1:WP_059175859.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
36% identity, 94% coverage: 4:239/251 of query aligns to 14:252/265 of P07821

query
sites
P07821

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K50 (= K40) mutation to Q: Lack of activity.
D172 (= D160) mutation to E: Lack of activity.
E173 (= E161) mutation to A: Lack of activity.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
36% identity, 87% coverage: 1:218/251 of query aligns to 2:218/241 of 4u00A

query
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4u00A

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binding adenosine-5'-diphosphate: F12 (≠ Y11), V17 (= V16), S37 (≠ N36), G38 (= G37), S39 (= S38), G40 (= G39), K41 (= K40), S42 (= S41), T43 (= T42)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
37% identity, 87% coverage: 1:218/251 of query aligns to 1:218/240 of 4ymuJ

query
sites
4ymuJ

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binding adenosine-5'-triphosphate: F11 (≠ Y11), V16 (= V16), S36 (≠ N36), G37 (= G37), S38 (= S38), G39 (= G39), K40 (= K40), S41 (= S41), T42 (= T42), E162 (= E161), H194 (= H194)
binding magnesium ion: S41 (= S41), E162 (= E161)

3c4jA Abc protein artp in complex with atp-gamma-s
33% identity, 87% coverage: 1:218/251 of query aligns to 3:220/242 of 3c4jA

query
sites
3c4jA

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binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y11), V18 (= V16), S38 (≠ N36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

3c41J Abc protein artp in complex with amp-pnp/mg2+
33% identity, 87% coverage: 1:218/251 of query aligns to 3:220/242 of 3c41J

query
sites
3c41J

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binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y11), V18 (= V16), S38 (≠ N36), G39 (= G37), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)
binding magnesium ion: S43 (= S41), D163 (= D160)

2olkA Abc protein artp in complex with adp-beta-s
33% identity, 87% coverage: 1:218/251 of query aligns to 3:220/242 of 2olkA

query
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2olkA

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A

G

Y

M

L

V

P

F

Q

Q

S

R

S

F

N

N

S

L

T

F

P

P

Y

H

S

M

L

T

V

V

F

L

D

N

A

N

I

I

L

T

L

L

G

A

R

P

K

M

P

-

H

K

I

V

S

R

F

K

E

W

P

P

S

R

E

E

R

K

D

A

L

E

E

A

V

K

V

A

E

M

R

E

I

L

E

D

D

K

F

V

G

G

L

L

K

K

D

D

F

K

A

A

F

H

R

A

K

Y

I

P

N

D

E

S

L

L

S

S

G

G

E

Q

V

A

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

M

E

E

P

P

Q

K

V

I

L

M

L

L

L

F

D

D

E

E

P

P

V

T

N

S

H

A

L

L

D

D

P

P

K

E

N

M

Q

V

I

G

E

E

I

V

L

L

K

S

I

V

L

M

K

K

K

Q

L

L

T

A

G

-

N

N

L

E

N

G

L

M

T

T

T

M

V

V

I

V

V

V

L

T

H

H

D

E

I

M

N

G

L

F

A

A

I

R

Q

E

F

V

A

G

D

D

Y

R

F

V

I

L

F

F

M

M

K

D

N

G

G

G

E

Y

I

I

Y

I

Q

E

T

E

G

G

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y11), V18 (= V16), S38 (≠ N36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

2oljA Abc protein artp in complex with adp/mg2+
33% identity, 87% coverage: 1:218/251 of query aligns to 3:220/242 of 2oljA

query
sites
2oljA

M

M

L

I

K

D

I

V

E

H

N

Q

L

L

T

K

A

K

G

S

Y
x
F

D

G

D

S

R

L

E

E

V
|
V

L

L

K

K

E

G

V

I

N

N

L

V

K

H

F

I

N

R

S

E

G

G

F

E

V

V

Y

V

A

V

I

V

L

I

G

G

P

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

T

C

I

L

D

N

R

L

I

L

L

E

K

D

P

F

K

D

K

E

G

G

K

E

V

I

Y

I

I

I

D

D

G

G

E

I

D

N

I

L

R

K

K

A

-

K

-

D

F

T

S

N

A

L

K

N

E

K

I

V

A

R

K

E

I

V

A

G

Y

M

L

V

P

F

Q

Q

S

R

S

F

N

N

S

L

T

F

P

P

Y

H

S

M

L

T

V

V

F

L

D

N

A

N

I

I

L

T

L

L

G

A

R

P

K

M

P

-

H

K

I

V

S

R

F

K

E

W

P

P

S

R

E

E

R

K

D

A

L

E

E

A

V

K

V

A

E

M

R

E

I

L

E

D

D

K

F

V

G

G

L

L

K

K

D

D

F

K

A

A

F

H

R

A

K

Y

I

P

N

D

E

S

L

L

S

S

G

G

E

Q

V

A

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

M

E

E

P

P

Q

K

V

I

L

M

L

L

L

F

D

D

E

E

P

P

V

T

N

S

H

A

L

L

D

D

P

P

K

E

N

M

Q

V

I

G

E

E

I

V

L

L

K

S

I

V

L

M

K

K

K

Q

L

L

T

A

G

-

N

N

L

E

N

G

L

M

T

T

T

M

V

V

I

V

V

V

L

T

H

H

D

E

I

M

N

G

L

F

A

A

I

R

Q

E

F

V

A

G

D

D

Y

R

F

V

I

L

F

F

M

M

K

D

N

G

G

G

E

Y

I

I

Y

I

Q

E

T

E

G

G

binding adenosine-5'-diphosphate: F13 (≠ Y11), V18 (= V16), S38 (≠ N36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
37% identity, 85% coverage: 1:214/251 of query aligns to 1:223/232 of 1f3oA

query
sites
1f3oA

M

M

L

I

K

K

I

L

E

K

N

N

L

V

T

T

A

K

G

T

Y
|
Y

D

K

D

M

R

G

E

E

-

E

-

I

-

Y

V

A

L

L

K

K

E

N

V

V

N

N

L

L

K

N

F

I

N

K

S

E

G

G

F

E

V

F

Y

V

A

S

I

I

L

M

G

G

P

P

N

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

T

I

I

I

D

G

R

C

I

L

L

D

K

K

P

P

K

T

K

E

G

G

K

E

V

V

Y

Y

I

I

D

D

G

N

E

I

D

K

I

T

R

N

K

D

F

L

S

D

A

D

K

D

E

E

I

L

A

T

K

K

-

I

-

R

-

D

K

K

I

I

A

G

Y

F

L

V

P

F

Q

Q

S

Q

S

F

N

N

S

L

T

I

P

P

Y

L

S

L

L

T

V

A

F

L

D

E

A

N

I

V

L

E

L

L

G

-

R

-

K

-

P

P

H

L

I

I

-

F

S

K

F

Y

E

R

P

G

S

A

E

M

R

S

D

G

L

E

E

E

V

R

E

K

R

R

I

A

I

L

E

E

D

C

F

L

G

K

L

M

K

A

D

E

-

L

-

E

-

R

F

F

A

A

F

N

R

H

K

K

I

P

N

N

E

Q

L

L

S

S

G

G

E

Q

V

Q

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

N

E

N

P

P

Q

P

V

I

L

I

L

L

L

A

D

D

E

E

P

P

V

T

N

G

H

A

L

L

D

D

P

S

K

K

N

T

Q

G

I

E

E

K

I

I

L

M

K

Q

I

L

L

L

K

K

L

L

T

N

G

E

N

E

L

D

N

G

L

K

T

T

T

V

V

V

I

V

V

V

L

T

H

H

D

D

I

I

N

N

L

V

A

A

I

-

Q

R

F

F

A

G

D

E

Y

R

F

I

I

I

F

Y

M

L

K

K

N

D

G

G

E

E

I

V

binding adenosine-5'-diphosphate: Y11 (= Y11), G41 (= G37), G43 (= G39), K44 (= K40), S45 (= S41), T46 (= T42)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
37% identity, 85% coverage: 1:214/251 of query aligns to 1:223/230 of 1l2tA

query
sites
1l2tA

M

M

L

I

K

K

I

L

E

K

N

N

L

V

T

T

A

K

G

T

Y
|
Y

D

K

D

M

R

G

E

E

-

E

-

I

-

Y

V

A

L

L

K

K

E

N

V

V

N

N

L

L

K

N

F

I

N

K

S

E

G

G

F

E

V

F

Y

V

A

S

I

I

L

M

G

G

P

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

T

I

I

I

D

G

R

C

I

L

L

D

K

K

P

P

K

T

K

E

G

G

K

E

V

V

Y

Y

I

I

D

D

G

N

E

I

D

K

I

T

R

N

K

D

F

L

S

D

A

D

K

D

E

E

I

L

A

T

K

K

-

I

-

R

-

D

K

K

I

I

A

G

Y

F

L

V

P

F

Q

Q

S

Q

S

F

N

N

S

L

T

I

P

P

Y

L

S

L

L

T

V

A

F

L

D

E

A

N

I

V

L

E

L

L

G

-

R

-

K

-

P

P

H

L

I

I

-

F

S

K

F

Y

E

R

P

G

S

A

E

M

R

S

D

G

L

E

E

E

V

R

E

K

R

R

I

A

I

L

E

E

D

C

F

L

G

K

L

M

K

A

D

E

-

L

-

E

-

R

F
|
F

A

A

F

N

R

H

K

K

I

P

N

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

E
x
Q

V

Q

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

N

E

N

P

P

Q

P

V

I

L

I

L

L

L

A

D

D

E

Q

P

P

V

T

N

G

H

A

L

L

D

D

P

S

K

K

N

T

Q

G

I

E

E

K

I

I

L

M

K

Q

I

L

L

L

K

K

L

L

T

N

G

E

N

E

L

D

N

G

L

K

T

T

T

V

V

V

I

V

V

V

L

T

H
|
H

D

D

I

I

N

N

L

V

A

A

I

-

Q

R

F

F

A

G

D

E

Y

R

F

I

I

I

F

Y

M

L

K

K

N

D

G

G

E

E

I

V

binding adenosine-5'-triphosphate: Y11 (= Y11), S40 (≠ N36), G41 (= G37), S42 (= S38), G43 (= G39), K44 (= K40), S45 (= S41), T46 (= T42), F138 (= F128), Q145 (≠ E135), S147 (= S137), G149 (= G139), Q150 (≠ E140), H204 (= H194)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
32% identity, 80% coverage: 19:218/251 of query aligns to 44:245/382 of 7ahhC

query
sites
7ahhC

E

D

V

T

N

N

L

F

K

E

F

I

N

N

S

E

G

G

F

E

V

I

Y

F

A

V

I

I

L

M

G

G

P

L

N

S

G
|
G

S

S

G
|
G

K
|
K

S

S

T

T

L

L

R

R

T

L

I

L

D

N

R

R

I

L

L

I

K

E

P

P

K

T

K

S

G

G

K

K

V

I

Y

F

I

I

D

D

G

N

E

Q

D

D

I

V

R

A

K

T

F

L

S

N

A

K

K

E

E

D

I

L

-

L

-

Q

-

V

-

R

A

R

K

K

K

T

I

M

A

S

Y

M

L

V

P

F

Q

Q

S

N

S

F

N

G

S

L

T

F

P

P

Y

H

S

R

L

T

V

I

F

L

D

E

A

N

I

T

L

E

L

Y

G

G

R

L

K

-

P

-

H

E

I

V

S

Q

F

N

E

V

P

P

S

K

E

E

R

E

D

R

L

R

E

K

V

R

V

A

E

E

R

K

I

A

I

L

E

D

D

N

F

A

G

N

L

L

K

L

D

D

F

F

A

K

F

D

R

Q

K

Y

I

P

N

K

E

Q

L

L

S

S

G

G

E

M

V

Q

Q

Q

K

R

V

V

L

G

I

L

A

A

R

R

A

A

L

L

A

A

Q

N

E

D

P

P

Q

E

V

I

L

L

L

M

D
|
D

E
|
E

P

A

V

F

N

S

H

A

L

L

D

D

P

P

K

L

N

I

Q

R

I

R

E

E

I

M

L

Q

K

D

I

E

L

L

K

L

K

E

L

L

T

Q

G

A

N

K

L

F

N

Q

L

K

T

T

T

I

V

I

I

F

V

V

L

S

H

H

D

D

I

L

N

N

L

E

A

A

I

L

Q

R

F

I

A

G

D

D

Y

R

F

I

I

A

F

I

M

M

K

K

N

D

G

G

E

K

I

I

Y

M

Q

Q

T

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: G62 (= G37), G64 (= G39), K65 (= K40), D187 (= D160), E188 (= E161)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
32% identity, 80% coverage: 19:218/251 of query aligns to 44:245/382 of 7aheC

query
sites
7aheC

E

D

V

T

N

N

L

F

K

E

F

I

N

N

S

E

G

G

F

E

V

I

Y

F

A

V

I

I

L

M

G

G

P

L

N

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

R

R

T

L

I

L

D

N

R

R

I

L

L

I

K

E

P

P

K

T

K

S

G

G

K

K

V

I

Y

F

I

I

D

D

G

N

E

Q

D

D

I

V

R

A

K

T

F

L

S

N

A

K

K

E

E

D

I

L

-

L

-

Q

-

V

-

R

A

R

K

K

K

T

I

M

A

S

Y

M

L

V

P

F

Q

Q

S

N

S

F

N

G

S

L

T

F

P

P

Y

H

S

R

L

T

V

I

F

L

D

E

A

N

I

T

L

E

L

Y

G

G

R

L

K

-

P

-

H

E

I

V

S

Q

F

N

E

V

P

P

S

K

E

E

R

E

D

R

L

R

E

K

V

R

V

A

E

E

R

K

I

A

I

L

E

D

D

N

F

A

G

N

L

L

K

L

D

D

F

F

A

K

F

D

R

Q

K

Y

I

P

N

K

E

Q

L

L

S

S

G

G

E

M

V

Q

Q

Q

K

R

V

V

L

G

I

L

A

A

R

R

A

A

L

L

A

A

Q

N

E

D

P

P

Q

E

V

I

L

L

L

M

D

D

E

E

P

A

V

F

N

S

H

A

L

L

D

D

P

P

K

L

N

I

Q

R

I

R

E

E

I

M

L

Q

K

D

I

E

L

L

K

L

K

E

L

L

T

Q

G

A

N

K

L

F

N

Q

L

K

T

T

T

I

V

I

I

F

V

V

L

S

H

H

D

D

I

L

N

N

L

E

A

A

I

L

Q

R

F

I

A

G

D

D

Y

R

F

I

I

A

F

I

M

M

K

K

N

D

G

G

E

K

I

I

Y

M

Q

Q

T

I

G

G

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
32% identity, 86% coverage: 1:216/251 of query aligns to 1:221/343 of P30750

query
sites
P30750

M

M

L

I

K

K

I

L

E

S

N

N

L

I

T

T

-

K

-

V

-

F

-

H

A

Q

G

G

Y

T

D

R

D

T

R

I

E

Q

V

A

L

L

K

N

E

N

V

V

N

S

L

L

K

H

F

V

N

P

S

A

G

G

F

Q

V

I

Y

Y

A

G

I

V

L

I

G

G

P

A

N
x
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

T

C

I

V

D

N

R

L

I

L

L

E

K

R

P

P

K

T

K

E

G

G

K

S

V

V

Y

L

I

V

D

D

G

G

E

Q

D

E

I

L

R

T

K

T

F

L

S

S

A

E

K

S

E

E

I

L

A

T

K

K

-

A

-

R

K

Q

I

I

A

G

Y

M

L

I

P

F

Q

Q

S

H

S

F

N

N

S

L

T

L

P

S

Y

S

S

R

L

T

V

V

F

F

D

G

A

N

I

V

L

A

L

L

G

P

R

-

K

-

P

-

H

-

I

L

S

E

F

L

E

D

P

N

S

T

E

P

R

K

D

D

L

-

E

E

V

V

V

K

E

R

R

R

I

V

I

T

E

E

D

L

F

L

-

S

-

L

-

V

G

G

L

L

K

G

D

D

F

K

A

H

F

D

R

S

K

Y

I

P

N

S

E

N

L

L

S

S

G

G

E

Q

V

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

E

N

P

P

Q

K

V

V

L

L

L

C

D

D

E
|
E

P

A

V

T

N

S

H

A

L

L

D

D

P

P

K

A

N

T

Q

T

I

R

E

S

I

I

L

L

K

E

I

L

L

L

K

K

K

D

L

I

T

N

G

R

N

R

L

L

N

G

L

L

T

T

T

I

V

L

I

L

V

I

L

T

H

H

D

E

I

M

N

D

L

V

A

V

I

K

Q

R

F

I

A

C

D

D

Y

C

F

V

I

A

F

V

M

I

K

S

N

N

G

G

E

E

I

L

Y

I

Q

E

40:46 (vs. 36:42, 71% identical) binding ATP
E166 (= E161) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 87% coverage: 1:218/251 of query aligns to 17:230/378 of P69874

query
sites
P69874

M

L

L

V

K

Q

I

L

E

A

N

G

L

I

T

R

A

K

G
x
C

Y
x
F

D

D

D

G

R

K

E

E

V

V

L

I

K

P

E

Q

V

L

N

D

L

L

K

T

F

I

N

N

S

N

G

G

F

E

V
x
F

Y

L

A

T

I

L

L

L

G

G

P

P

N

S

G

G

S
x
C

G

G

K

K

S

T

T

T

L

V

L
|
L

R

R

T

L

I

I

D

A

R

G

I

L

L

E

K

T

P

V

K

D

K

S

G

G

K

R

V

I

Y

M

I
x
L

D

D

G

N

E

E

D

D

I

I

R

T

K

H

F

V

S

P

A

A

K

E

E

N

I

-

A

-

K

R

K

Y

I

V

A

N

Y

T

L

V

P

F

Q

Q

S

S

S

Y

N

A

S

L

T

F

P

P

Y

H

S

M

L

T

V

V

F

F

D

E

A

N

I

V

L

A

L

F

G

G

R

L

K

R

P

-

H

-

I

M

S

Q

F

K

E

T

P

P

S

A

E

-

R

-

D

-

L

A

E

E

V

I

V

T

E

P

R

R

I

V

I

M

E

E

D

A

F

L

-

R

-

M

-
x
V

G

Q

L

L

K

E

D

T

F

F

A

A

F

Q

R

R

K

K

I

P

N

H

E

Q

L

L

S

S

G

G

E

Q

V

Q

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

V

A

V

Q

N

E

K

P

P

Q

R

V

L

L

L

D
|
D

E

E

P

S

V

L

N

S

H

A

L

L

D

D

P

Y

K

K

N

L

Q

R

I

K

E

Q

I

M

L

Q

K

N

I

E

L

L

K

K

K

A

L

L

T

Q

G

R

N

K

L

L

N

G

L

I

T

T

T

F

V

V

I

F

V

V

L

T

H

H

D

D

I

Q

N

E

L

E

A

A

I

L

Q

T

F

M

A

S

D

D

Y

R

F

I

I

V

F

V

M

M

K

R

N

D

G

G

E

R

I

I

Y

E

Q

Q

T

D

G

G

C26 (≠ G10) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y11) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ V29) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S38) mutation to T: Loss of ATPase activity and transport.
L60 (= L44) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I60) mutation to P: Lower ATPase activity and transport efficiency.
V135 (vs. gap) mutation to M: Loss of ATPase activity and transport.
D172 (= D160) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
32% identity, 86% coverage: 1:216/251 of query aligns to 2:222/344 of 3tuzC

query
sites
3tuzC

M

M

L

I

K

K

I

L

E

S

N

N

L

I

T

T

-

K

-

V

-
x
F

-

H

A
x
Q

G

G

Y

T

D

R

D

T

R

I

E

Q

V

A

L

L

K

N

E

N

V

V

N

S

L

L

K

H

F

V

N

P

S

A

G

G

F

Q

V

I

Y

Y

A

G

I

V

L

I

G

G

P

A

N

S

G
|
G

S

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

T

C

I

V

D

N

R

L

I

L

L

E

K

R

P

P

K

T

K

E

G

G

K

S

V

V

Y

L

I

V

D

D

G

G

E

Q

D

E

I

L

R

T

K

T

F

L

S

S

A

E

K

S

E

E

I

L

A

T

K

K

-

A

-

R

K

Q

I

I

A

G

Y

M

L

I

P

F

Q

Q

S

H

S

F

N

N

S

L

T

L

P

S

Y

S

S

R

L

T

V

V

F

F

D

G

A

N

I

V

L

A

L

L

G

P

R

-

K

-

P

-

H

-

I

L

S

E

F

L

E

D

P

N

S

T

E

P

R

K

D

D

L

-

E

E

V

V

V

K

E

R

R

R

I

V

I

T

E

E

D

L

F

L

-

S

-

L

-

V

G

G

L

L

K

G

D

D

F

K

A

H

F

D

R

S

K

Y

I

P

N

S

E

N

L

L

S

S

G

G

E

Q

V

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

E

N

P

P

Q

K

V

V

L

L

L

C

D

D

E

Q

P

A

V

T

N

S

H

A

L

L

D

D

P

P

K

A

N

T

Q

T

I

R

E

S

I

I

L

L

K

E

I

L

L

L

K

K

K

D

L

I

T

N

G

R

N

R

L

L

N

G

L

L

T

T

T

I

V

L

I

L

V

I

L

T

H

H

D

E

I

M

N

D

L

V

A

V

I

K

Q

R

F

I

A

C

D

D

Y

C

F

V

I

A

F

V

M

I

K

S

N

N

G

G

E

E

I

L

Y

I

Q

E

binding adenosine-5'-diphosphate: F12 (vs. gap), Q14 (≠ A9), G42 (= G37), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
32% identity, 86% coverage: 1:216/251 of query aligns to 2:222/344 of 3tuiC

query
sites
3tuiC

M

M

L

I

K

K

I

L

E

S

N

N

L

I

T

T

-

K

-

V

-
x
F

-

H

A

Q

G

G

Y

T

D

R

D

T

R

I

E

Q

V

A

L

L

K

N

E

N

V

V

N

S

L

L

K

H

F

V

N

P

S

A

G

G

F

Q

V

I

Y

Y

A

G

I

V

L

I

G

G

P

A

N

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

T

C

I

V

D

N

R

L

I

L

L

E

K

R

P

P

K

T

K

E

G

G

K

S

V

V

Y

L

I

V

D

D

G

G

E

Q

D

E

I

L

R

T

K

T

F

L

S

S

A

E

K

S

E

E

I

L

A

T

K

K

-

A

-

R

K

Q

I

I

A

G

Y

M

L

I

P

F

Q

Q

S

H

S

F

N

N

S

L

T

L

P

S

Y

S

S

R

L

T

V

V

F

F

D

G

A

N

I

V

L

A

L

L

G

P

R

-

K

-

P

-

H

-

I

L

S

E

F

L

E

D

P

N

S

T

E

P

R

K

D

D

L

-

E

E

V

V

V

K

E

R

R

R

I

V

I

T

E

E

D

L

F

L

-

S

-

L

-

V

G

G

L

L

K

G

D

D

F

K

A

H

F

D

R

S

K

Y

I

P

N

S

E

N

L

L

S

S

G

G

E

Q

V

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

E

N

P

P

Q

K

V

V

L

L

L

C

D

D

E

Q

P

A

V

T

N

S

H

A

L

L

D

D

P

P

K

A

N

T

Q

T

I

R

E

S

I

I

L

L

K

E

I

L

L

L

K

K

K

D

L

I

T

N

G

R

N

R

L

L

N

G

L

L

T

T

T

I

V

L

I

L

V

I

L

T

H

H

D

E

I

M

N

D

L

V

A

V

I

K

Q

R

F

I

A

C

D

D

Y

C

F

V

I

A

F

V

M

I

K

S

N

N

G

G

E

E

I

L

Y

I

Q

E

binding adenosine-5'-diphosphate: F12 (vs. gap), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42)

7ahdC Opua (e190q) occluded (see paper)
32% identity, 80% coverage: 19:218/251 of query aligns to 44:245/260 of 7ahdC

query
sites
7ahdC

E

D

V

T

N

N

L

F

K

E

F

I

N

N

S

E

G

G

F

E

V

I

Y

F

A

V

I

I

L

M

G

G

P

L

N
x
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

L

I

L

D

N

R

R

I

L

L

I

K

E

P

P

K

T

K

S

G

G

K

K

V

I

Y

F

I

I

D

D

G

N

E

Q

D

D

I

V

R

A

K

T

F

L

S

N

A

K

K

E

E

D

I

L

-

L

-

Q

-

V

-

R

A

R

K

K

K

T

I

M

A

S

Y

M

L

V

P

F

Q
|
Q

S

N

S

F

N

G

S

L

T

F

P

P

Y

H

S

R

L

T

V

I

F

L

D

E

A

N

I

T

L

E

L

Y

G

G

R

L

K

E

P

-

H

-

I

V

S

Q

F

N

E

V

P

P

S

K

E

E

R

E

D

R

L

R

E

K

V

R

V

A

E

E

R

K

I

A

I

L

E

D

D

N

F

A

G

N

L

L

K

L

D

D

F

F

A

K

F

D

R

Q

K

Y

I

P

N
x
K

E
x
Q

L

L

S
|
S

G

G

G
|
G

E
x
M

V

Q

Q

Q

K

R

V

V

L

G

I

L

A

A

R

R

A

A

L

L

A

A

Q

N

E

D

P

P

Q

E

V

I

L

L

L

M

D

D

E
x
Q

P

A

V

F

N

S

H

A

L

L

D

D

P

P

K

L

N

I

Q

R

I

R

E

E

I

M

L

Q

K

D

I

E

L

L

K

L

K

E

L

L

T

Q

G

A

N

K

L

F

N

Q

L

K

T

T

T

I

V

I

I

F

V

V

L

S

H
|
H

D

D

I

L

N

N

L

E

A

A

I

L

Q

R

F

I

A

G

D

D

Y

R

F

I

I

A

F

I

M

M

K

K

N

D

G

G

E

K

I

I

Y

M

Q

Q

T

I

G

G

binding adenosine-5'-triphosphate: S61 (≠ N36), G62 (= G37), G64 (= G39), K65 (= K40), S66 (= S41), T67 (= T42), Q111 (= Q82), K161 (≠ N134), Q162 (≠ E135), S164 (= S137), G166 (= G139), M167 (≠ E140), Q188 (≠ E161), H221 (= H194)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 39

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
32% identity, 86% coverage: 1:216/251 of query aligns to 2:222/344 of 6cvlD

query
sites
6cvlD

M

M

L

I

K

K

I

L

E

S

N

N

L

I

T

T

-

K

-

V

-
x
F

-

H

A
x
Q

G

G

Y

T

D

R

D

T

R
x
I

E

Q

V

A

L

L

K

N

E

N

V

V

N

S

L

L

K

H

F

V

N

P

S

A

G

G

F

Q

V

I

Y

Y

A

G

I

V

L

I

G

G

P

A

N
x
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

T

C

I

V

D

N

R

L

I

L

L

E

K

R

P

P

K

T

K

E

G

G

K

S

V

V

Y

L

I

V

D

D

G

G

E

Q

D

E

I

L

R

T

K

T

F

L

S

S

A

E

K

S

E

E

I

L

A

T

K

K

-

A

-

R

K

Q

I

I

A

G

Y

M

L

I

P

F

Q

Q

S

H

S

F

N

N

S

L

T

L

P

S

Y

S

S

R

L

T

V

V

F

F

D

G

A

N

I

V

L

A

L

L

G

P

R

-

K

-

P

-

H

-

I

L

S

E

F

L

E

D

P

N

S

T

E

P

R

K

D

D

L

-

E

E

V

V

V

K

E

R

R

R

I

V

I

T

E

E

D

L

F

L

-

S

-

L

-

V

G

G

L

L

K

G

D

D

F

K

A

H

F

D

R

S

K

Y

I

P

N

S

E
x
N

L

L

S
|
S

G

G

E
x
Q

V

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

E

N

P

P

Q

K

V

V

L

L

L

C

D

D

E

Q

P

A

V

T

N

S

H

A

L

L

D

D

P

P

K

A

N

T

Q

T

I

R

E

S

I

I

L

L

K

E

I

L

L

L

K

K

K

D

L

I

T

N

G

R

N

R

L

L

N

G

L

L

T

T

T

I

V

L

I

L

V

I

L

T

H
|
H

D

E

I

M

N

D

L

V

A

V

I

K

Q

R

F

I

A

C

D

D

Y

C

F

V

I

A

F

V

M

I

K

S

N

N

G

G

E

E

I

L

Y

I

Q

E

binding phosphothiophosphoric acid-adenylate ester: F12 (vs. gap), Q14 (≠ A9), I19 (≠ R14), S41 (≠ N36), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42), N141 (≠ E135), S143 (= S137), Q146 (≠ E140), H200 (= H194)

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
33% identity, 86% coverage: 2:218/251 of query aligns to 7:225/375 of 2d62A

query
sites
2d62A

L

V

K

K

I

L

E

I

N

N

L

I

T

W

A

K

G

R

Y

F

D

G

D

D

R

V

E

T

V

A

L

V

K

K

E

D

V

L

N

S

L

L

K

E

F

I

N

K

S

D

G

G

F

E

V

F

Y

L

A

V

I

L

L

L

G

G

P

P

N

S

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

R

R

T

M

I

I

D

A

R

G

I

L

L

E

K

E

P

P

K

T

K

R

G

G

K

Q

V

I

Y

Y

I

I

-

E

-

D

-

N

-

L

-

V

-

A

D

D

G

P

E

E

D

K

I

G

R

V

K

F

F

V

S

P

A

P

K

K

E

E

I

-

A

-

K

R

K

D

I

V

A

A

Y

M

L

V

P

F

Q

Q

S

S

S

Y

N

A

S

L

T

Y

P

P

Y

H

S

M

L

T

V

V

F

Y

D

D

A

N

I

I

L

A

L

F

G

P

R

L

K

K

P

-

H

-

I

L

S

R

F

K

E

V

P

P

S

K

E

Q

R

E

D

I

L

D

E

K

V

R

V

V

E

R

R

E

I

V

I

A

E

E

D

M

F

L

G

G

L

L

K

T

D

E

F

L

A

L

F

N

R

R

K

K

I

P

N

R

E

E

L

L

S

S

G

G

E

Q

V

R

Q

Q

K

R

V

V

L

A

I

L

A

G

R

R

A

A

L

I

A

I

Q

R

E

R

P

P

Q

K

V

V

L

F

L

L

L

M

D

D

E

E

P

P

V

L

N

S

H

N

L

L

D

D

P

A

K

K

N

L

Q

R

I

V

E

K

I

M

L

R

K

A

I

E

L

L

K

K

L

L

T

Q

G

R

N

Q

L

L

N

G

L

V

T

T

V

I

I

Y

V

V

L

T

H

H

D

D

I

Q

N

V

L

E

A

A

I

M

Q

T

F

M

A

G

D

D

Y

R

F

I

I

A

F

V

M

M

K

N

N

K

G

G

E

E

I

L

Y

Q

Q

Q

T

V

G

G

binding pyrophosphate 2-: G42 (= G37), C43 (≠ S38), G44 (= G39), K45 (= K40), T46 (≠ S41), T47 (= T42)

1g291 Malk (see paper)
34% identity, 80% coverage: 17:218/251 of query aligns to 19:222/372 of 1g291

query
sites
1g291

L

V

K

R

E

E

V

M

N

S

L

L

K

E

F

V

N

K

S

D

G

G

F

E

V

F

Y

M

A

I

I

L

L

L

G

G

P

P

N
x
S

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

R

R

T

M

I

I

D

A

R

G

I

L

L

E

K

E

P

P

K

S

K

R

G

G

K

Q

V

I

Y

Y

I

I

D

-

G

G

E

D

D

K

I

L

R

V

K

A

F
x
D

S

P

A
x
E

K
|
K

E

G

I

I

-

F

-

V

-

P

-
x
K

A
x
D

K

R

K

D

I

I

A

A

Y

M

L

V

P

F

Q

Q

S

S

S

Y

N

A

S

L

T

Y

P

P

Y

H

S

M

L

T

V

V

F

Y

D

D

A

N

I

I

L

A

L

F

G

P

R

L

K

K

P

-

H

-

I

L

S

R

F

K

E

V

P

P

S

R

E

Q

R

E

D

I

L

D

E

Q

V

R

V

V

E

R

R

E

I

V

I

A

E

E

D

L

F

L

G

G

L

L

K

T

D

E

F

L

A

L

F

N

R

R

K

K

I

P

N

R

E

E

L

L

S

S

G

G

E

Q

V

R

Q

Q

K

R

V

V

L

A

I

L

A

G

R

R

A

A

L

I

A

V

Q

R

E

K

P

P

Q

Q

V

V

L

F

L

L

L

M

D

D

E

E

P

P

V

L

N

S

H

N

L

L

D

D

P

A

K

K

N

L

Q

R

I

V

E

R

I

M

L

R

K

A

I

E

L

L

K

K

L

L

T

Q

G

R

N

Q

L

L

N

G

L

V

T

T

V

I

I

Y

V

V

L

T

H

H

D

D

I

Q

N

V

L

E

A

A

I

M

Q

T

F

M

A

G

D

D

Y

R

F

I

I

A

F

V

M

M

K

N

N

R

G

G

E

V

I

L

Y

Q

Q

Q

T

V

G

G

binding magnesium ion: D69 (≠ F68), E71 (≠ A70), K72 (= K71), K79 (vs. gap), D80 (≠ A74)
binding pyrophosphate 2-: S38 (≠ N36), G39 (= G37), C40 (≠ S38), G41 (= G39), K42 (= K40), T43 (≠ S41), T44 (= T42)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

7d0aB Acinetobacter mlafedb complex in adp-vanadate trapped vclose conformation (see paper)
32% identity, 87% coverage: 1:218/251 of query aligns to 4:223/263 of 7d0aB

query
sites
7d0aB

M

L

L

I

K

E

I

V

E

K

N

N

L

L

T

S

A

F

G

N

Y

R

D

G

D

E

R

R

E

V

V

I

L

Y

K

D

E

N

V

I

N

S

L

L

K

N

F

I

N

R

S

R

G

G

F

Q

V

I

Y

T

A

A

I

I

L

M

G

G

P

P

N
x
S

G

G

S
x
T

G
|
G

K
|
K

S

T

T

T

L

L

R

R

T

L

I

I

D

G

R

G

I

Q

L

L

K

V

P

P

K

D

K

Q

G

G

K

E

V

V

Y

L

I

L

D

D

G

G

E

K

D

D

I

I

R

A

K

Q

F

M

S

S

A

R

K

Q

E

E

I

L

-

F

-

A

A

R

K

A

K

R

I

M

A

G

Y

M

L

L

P

F

Q

Q

S

S

S

G

N

A

S

L

T

F

P

T

Y

D

S

M

L

S

V

V

F

Y

D

E

A

N

I

V

L

A

L

F

G

P

R

I

K

R

P

A

H

H

I

T

S

K

F

L

E

-

P

-

S

S

E

E

R

N

D

L

L

I

-

A

E

E

V

L

V

V

E

A

R

L

I

K

I

L

E

E

D

S

F

V

G

G

L

L

K

R

D

G

F

T

A

E

F

Q

R

L

K

M

I

P

N

T

E
|
E

L

L

S
|
S

G

G

E
x
M

V

N

Q

R

K

R

V

V

L

A

I

L

A

A

R

R

A

A

L

I

A

A

Q

L

E

D

P

P

Q

D

V

L

L

I

L

M

L

Y

D

D

E

E

P

P

V

F

N

A

H

G

L

Q

D

D

P

P

K

I

N

V

Q

K

I

G

E

V

I

L

L

T

K

R

I

L

L

I

K

R

K

S

L

L

T

R

G

E

N

A

L

L

N

D

L

L

T

T

V

I

I

I

V

V

L

S

H

H

D

D

I

V

N

P

L

E

A

T

I

L

Q

S

F

I

A

A

D

D

Y

Y

F

I

I

Y

F

V

M

V

K

A

N

E

G

G

E

K

I

I

Y

Q

Q

G

T

E

G

G

binding adenosine-5'-diphosphate: S39 (≠ N36), T41 (≠ S38), G42 (= G39), K43 (= K40), E140 (= E135), S142 (= S137), M145 (≠ E140)

Query Sequence

>WP_059175859.1 NCBI__GCF_001514535.1:WP_059175859.1
MLKIENLTAGYDDREVLKEVNLKFNSGFVYAILGPNGSGKSTLLRTIDRILKPKKGKVYI
DGEDIRKFSAKEIAKKIAYLPQSSNSTPYSLVFDAILLGRKPHISFEPSERDLEVVERII
EDFGLKDFAFRKINELSGGEVQKVLIARALAQEPQVLLLDEPVNHLDPKNQIEILKILKK
LTGNLNLTTVIVLHDINLAIQFADYFIFMKNGEIYQTGDCKIIEPSLIKDVYDIDVKIIE
LDRRKFVISDF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory