SitesBLAST
Comparing WP_061532371.1 NCBI__GCF_001584165.1:WP_061532371.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P76558 NADP-dependent malic enzyme; NADP-ME; EC 1.1.1.40 from Escherichia coli (strain K12) (see paper)
62% identity, 98% coverage: 11:766/770 of query aligns to 1:754/759 of P76558
- K56 (≠ N66) modified: N6-acetyllysine
6zngF Maeb full-length acetyl-coa bound state (see paper)
47% identity, 97% coverage: 17:766/770 of query aligns to 6:743/753 of 6zngF
- active site: Y38 (= Y49), A74 (= A85), K93 (= K104), E135 (= E146), D136 (= D147), D160 (= D171), D161 (= D172), N286 (= N297)
- binding acetyl coenzyme *a: R511 (≠ T530), K514 (≠ Q533), Y552 (= Y578), A553 (≠ I579), R557 (≠ L583), L560 (≠ R586), P571 (vs. gap), T590 (≠ H611), V591 (= V612), N592 (= N613), L593 (≠ E614), Y625 (≠ H646), Q659 (≠ H681), L690 (≠ I712), N694 (= N716), Q724 (≠ T747)
6zn4A Maeb malic enzyme domain apoprotein (see paper)
59% identity, 52% coverage: 17:417/770 of query aligns to 5:405/406 of 6zn4A
6zn7A Maeb malic enzyme domain apoprotein (see paper)
59% identity, 52% coverage: 17:417/770 of query aligns to 5:405/405 of 6zn7A
- active site: Y37 (= Y49), A73 (= A85), K92 (= K104), E134 (= E146), D135 (= D147), D159 (= D171), D160 (= D172), N285 (= N297)
- binding magnesium ion: E134 (= E146), D135 (= D147), D160 (= D172)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T164 (= T176), N191 (≠ S203), A193 (= A205), G194 (= G206), A195 (= A207), S196 (≠ A208), D218 (= D230), S219 (≠ L231), K235 (= K247), L260 (= L272), S261 (= S273), V262 (≠ A274), M283 (≠ L295), N285 (= N297), V315 (= V327)
5ceeA Malic enzyme from candidatus phytoplasma aywb in complex with NAD and mg2+ (see paper)
46% identity, 52% coverage: 15:414/770 of query aligns to 1:385/387 of 5ceeA
- active site: Y34 (= Y49), A70 (= A85), K89 (= K104), E131 (= E146), D132 (= D147), D156 (= D171), D157 (= D172), N283 (= N297)
- binding magnesium ion: E131 (= E146), D132 (= D147), D157 (= D172)
- binding nicotinamide-adenine-dinucleotide: T161 (= T176), N188 (≠ S203), G189 (= G204), G191 (= G206), A193 (= A208), D213 (= D230), K214 (≠ L231), V258 (≠ L272), S259 (= S273), I263 (≠ V277), L281 (= L295), N283 (= N297), V312 (= V327), N314 (= N329)
2dvmA NAD complex structure of ph1275 protein from pyrococcus horikoshii
45% identity, 54% coverage: 15:432/770 of query aligns to 1:416/438 of 2dvmA
- active site: Y37 (= Y49), R73 (≠ A85), K92 (= K104), E134 (= E146), D135 (= D147), D159 (= D171), D160 (= D172), N296 (= N297)
- binding nicotinamide-adenine-dinucleotide: T164 (= T176), G194 (= G206), A195 (= A207), A196 (= A208), V217 (≠ T229), E218 (≠ D230), L219 (= L231), P224 (≠ G238), F269 (≠ L272), T270 (≠ S273), L294 (= L295), N296 (= N297), N327 (= N329)
2a9fA Crystal structure of a putative malic enzyme ((s)-malate:nad+ oxidoreductase (decarboxylating))
49% identity, 46% coverage: 11:363/770 of query aligns to 1:350/383 of 2a9fA
2haeD Crystal structure of a putative malic enzyme (malate oxidoreductase)
43% identity, 51% coverage: 19:409/770 of query aligns to 2:373/373 of 2haeD
2haeB Crystal structure of a putative malic enzyme (malate oxidoreductase)
43% identity, 51% coverage: 19:409/770 of query aligns to 2:373/373 of 2haeB
- active site: Y31 (= Y49), A67 (= A85), K86 (= K104), E128 (= E146), D129 (= D147), D153 (= D171), D154 (= D172), N280 (= N297)
- binding nicotinamide-adenine-dinucleotide: T158 (= T176), N185 (≠ S203), G188 (= G206), A189 (= A207), A190 (= A208), D210 (= D230), R211 (≠ L231), V255 (≠ L272), S256 (= S273), R257 (≠ A274), L278 (= L295), A279 (= A296), N280 (= N297), N311 (= N329)
1xcoD Crystal structure of a phosphotransacetylase from bacillus subtilis in complex with acetylphosphate (see paper)
31% identity, 43% coverage: 440:767/770 of query aligns to 3:325/325 of 1xcoD