SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_061533209.1 NCBI__GCF_001584165.1:WP_061533209.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

P25080 Urocanate hydratase; Urocanase; Imidazolonepropionate hydrolase; EC 4.2.1.49 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 4 papers)
74% identity, 99% coverage: 7:566/567 of query aligns to 2:555/557 of P25080

query
sites
P25080

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GG 53:54 (= GG 58:59) binding NAD(+)
C64 (= C69) mutation to A: No loss of activity.
Q131 (= Q136) binding NAD(+)
GMG 177:179 (= GMG 182:184) binding NAD(+)
C192 (≠ A197) mutation to A: No loss of activity.
ECQQSR 197:202 (≠ ECKQSS 202:207) binding NAD(+)
C198 (= C203) mutation to A: No loss of activity.
NA 243:244 (= NA 248:249) binding NAD(+)
QTSAH 264:268 (= QTSAH 273:277) binding NAD(+)
YL 274:275 (= YL 283:284) binding NAD(+)
YG 323:324 (= YG 334:335) binding NAD(+)
C355 (= C366) mutation to A: Minor loss in activity.
C411 (= C422) mutation to A: Loss of activity.
RE 455:456 (= RE 466:467) binding NAD(+)
G493 (= G504) binding NAD(+)
C544 (= C555) mutation to A: No loss of activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1uwkA The high resolution structure of urocanate hydratase from pseudomonas putida in complex with urocanate (see paper)
75% identity, 97% coverage: 17:566/567 of query aligns to 9:552/554 of 1uwkA

query
sites
1uwkA

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binding nicotinamide-adenine-dinucleotide: Y49 (= Y57), G50 (= G58), G51 (= G59), I142 (= I150), G173 (= G181), G174 (= G182), M175 (= M183), G176 (= G184), E194 (= E202), S195 (≠ C203), Q196 (≠ K204), N240 (= N248), A241 (= A249), Q261 (= Q273), T262 (= T274), S263 (= S275), H265 (= H277), Y271 (= Y283), L272 (= L284), W278 (≠ V290), Y320 (= Y334), G321 (= G335), N322 (= N336), F342 (= F356), G490 (= G504)
binding (2e)-3-(1h-imidazol-4-yl)acrylic acid: Y49 (= Y57), M129 (= M137), T130 (= T138), G141 (= G149), M175 (= M183), R359 (= R373), D440 (= D454)

7jfzA Structure of urocanate hydratase from legionella pneumophila bound to NAD
68% identity, 97% coverage: 15:565/567 of query aligns to 1:545/547 of 7jfzA

query
sites
7jfzA

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K

K

P

P

V

V

G

G

V

V

F

F

Q

T

T

T

H

H

A

E

D

E

A

A

P

P

R

R

V

I

L

L

L

I

A

A

N

N

S

S

N

N

L

L

V

V

P

P

K

R

W

W

A

A

N

T

W

W

E

E

H

H

F

F

N

N

E

E

L

L

D

D

R

K

K

K

G

G

L

L

F

M

M

M

Y

Y

G

G

Q

Q

M

M

T

T

A

A

G

G

S

S

W

W

I

I

Y

Y

I

I

G

G

S

S

Q

Q

G

G

I

I

V

V

Q

Q

G

G

T

T

F

Y

E

E

T

T

F

F

A

V

E

A

A

A

G

A

R

K

Q

K

H

H

F

Y

G

Q

G

G

D

D

L

L

S

S

G

G

R

R

W

W

I

I

L

L

T

T

A

A

G

G

L

L

G
|
G

G
|
G

M
|
M

G

G

G

G

A

A

Q

Q

P

P

L

L

A

A

A

G

T

T

L

M

A

A

G

G

A

A

A

S

S

V

L

L

T

A

I

V

E
|
E

C
|
C

K

D

Q

R

S

Q

S
x
R

I

I

D

E

F

K

R

R

L

L

R

Q

T

T

R

K

Y

Y

L

L

D

D

K

R

Q

Y

A

T

K

D

N

N

L

L

D

S

D

E

A

A

L

L

D

D

L

W

I

I

K

N

Y

E

H

S

T

C

E

R

R

R

K

K

E

K

A

P

I

V

S

S

I

V

G

A

L

V

L

L

G

G

N
|
N

A
|
A

A

A

E

E

V

I

L

F

P

P

E

Q

L

L

V

V

R

K

R

L

A

-

K

-

A

-

G

-

G

G

I

V

K

Q

P

P

D

S

L

L

V

V

T

T

D

D

Q
|
Q

T
|
T

S
|
S

A

A

H
|
H

D

D

L

P

I

L

N

N

G

G

Y
|
Y

L
|
L

P

P

I

L

G

G

W

W

S

T

V

L

E

E

Q

Q

W

A

V

V

A

E

A

M

Q

R

N

K

D

K

P

S

S

P

Q

E

Q

E

A

-

R

-

L

-

T

V

A

V

D

D

A

A

A

A

H

K

-

K

S

S

C

M

A

A

Q

V

H

Q

V

V

Q

H

A

A

M

M

L

L

D

E

F

F

H

H

A

N

M

R

G

G

I

I

K

P

T

V

V

F

D

D

Y
|
Y

G
|
G

N
|
N

N

N

I

I

R

R

Q

Q

V

M

A

A

F

F

D

E

Q

A

G

G

V

E

K

K

N

N

A

A

F

F

D

S

F

F

P

E

G

G

F
|
F

V

V

P

P

A

A

Y

Y

I

I

R

R

P

P

L

L

F

F

C

C

E

E

G

G

K

I

G

G

P

P

F

F

R

R

W

W

V

V

A

A

L

L

S

S

G

G

D

D

P

P

E

E

D

D

I

I

Y

Y

K

A

T

T

D

D

A

E

K

R

I

V

K

K

E

Q

L

L

F

I

P

P

E

D

N

A

K

P

H

H

V

L

H

H

R

H

W

W

L

L

D

D

M

M

A

A

H

R

E

E

R

K

I

I

A

S

F

F

Q

Q

G

G

L

L

P

P

A

A

R

R

I

I

C

C

W

W

L

V

G

G

L

L

G

K

E

D

R

R

H

A

I

R

A

L

G

A

L

L

A

A

F

F

N

N

E

E

M

M

V

V

R

K

N

N

G

K

E

Q

L

V

K

K

A

A

P

P

V

I

V

V

I

I

G

G

R

R

D

D

H

H

L

L

D

D

T

S

G

G

S

S

V

V

A

A

S

S

P

P

N

N

R
|
R

E

E

T

T

E

E

S

G

M

M

K

L

D

D

G

G

S

S

D

D

A

A

V

V

S

S

D

D

W

W

P

P

L

L

L

L

N

N

A

A

L

L

L

L

N

N

T

C

S

A

G

S

G

G

A

A

T

T

W

W

V

V

S

S

L

I

H

H

H

H

G

G

G

G

G

G

V

V

G

G

M

M

G

G

Y

F

S

S

Q

Q

H

H

A

A

G

G

M

V

V

V

I

I

V

V

A

A

D

D

G

G

T

T

D

E

A

K

A

A

A

A

K

K

R

R

L

L

A

A

R

R

V

V

L

L

V

H

N

N

D

D

C

P

A

A

S

T

G

G

V

V

M

M

R

R

H

H

A

A

D

D

A

A

G

G

Y

Y

E

Q

A

I

A

A

V

K

E

Q

C

C

A

A

K

K

R

E

H

N

N

S

L

L

N

W

L

L

P

P

M

M

binding nicotinamide-adenine-dinucleotide: G167 (= G181), G168 (= G182), M169 (= M183), E188 (= E202), C189 (= C203), R193 (≠ S207), N234 (= N248), A235 (= A249), Q255 (= Q273), T256 (= T274), S257 (= S275), H259 (= H277), Y265 (= Y283), L266 (= L284), Y314 (= Y334), G315 (= G335), N316 (= N336), F336 (= F356), R446 (= R466)

P25503 Urocanate hydratase; Urocanase; Imidazolonepropionate hydrolase; EC 4.2.1.49 from Bacillus subtilis (strain 168)
64% identity, 98% coverage: 12:567/567 of query aligns to 3:552/552 of P25503

query
sites
P25503

D

D

P

V

S

K

R

K

T

S

I

I

R

R

A

A

A

N

R

R

G

G

T

T

Q

E

L

L

T

E

A

C

K

L

S

G

W

W

L

E

T

Q

E

E

A

A

P

V

L

L

R

R

M

M

L

L

M

R

N

N

N

N

L

L

D

D

P

P

E

E

V

V

A

A

E

E

R

K

P

P

N

E

D

D

L

L

V

I

V

V

Y

Y

G
|
G

G
|
G

I

I

G

G

R

K

A

A

A

A

R

R

D

D

W

W

A

D

C

A

F

F

D

H

K

A

I

I

V

E

E

H

A

S

L

L

T

K

K

T

L

L

N

K

D

N

D

D

E

E

T

T

L

L

L

L

I

V

Q

Q

S

S

G

G

K

K

P

P

V

V

G

G

V

M

F

F

Q

R

T

T

H

H

A

P

D

Q

A

A

P

P

R

R

V

V

L

L

L

L

A

A

N

N

S

S

N

V

L

L

V

V

P

P

K

K

W

W

A

A

N

D

W

W

E

E

H

H

F

F

N

H

E

E

L

L

D

E

R

K

K

K

G

G

L

L

F

M

M

M

Y

Y

G

G

Q
|
Q

M

M

T

T

A

A

G

G

S

S

W

W

I

I

Y

Y

I

I

G

G

S

S

Q

Q

G

G

I

I

V

L

Q

Q

G

G

T

T

F

Y

E

E

T

T

F

F

A

A

E

E

A

L

G

A

R

R

Q

Q

H

H

F

F

G

G

G

G

D

S

L

L

S

K

G

G

R

T

W

L

I

T

L

L

T

T

A

A

G

G

L

L

G

G

G
|
G

M
|
M

G
|
G

G

G

A

A

Q

Q

P

P

L

L

A

S

A

V

T

T

L

M

A

N

G

E

A

G

A

V

S

V

L

I

T

A

I

V

E
|
E

C

V

K

D

Q

E

S

K

S
x
R

I

I

D

D

F

K

R

R

L

I

R

E

T

T

R

K

Y

Y

L

C

D

D

K

R

Q

K

A

T

K

A

N

S

L

I

D

E

D

E

A

A

L

L

D

A

L

W

I

A

K

E

Y

E

H

A

T

K

E

L

R

A

K

G

E

K

A

P

I

L

S

S

I

I

G

A

L

L

L

L

G

G

N
|
N

A
|
A

A

A

E

E

V

V

L

H

P

H

E

T

L

L

V

L

R

N

R

R

A

-

K

-

A

-

G

-

G

G

I

V

K

K

P

I

D

D

L

I

V

V

T

T

D

D

Q
|
Q

T
|
T

S
|
S

A
|
A

H
|
H

D

D

L

P

I

L

N

I

G

G

Y
|
Y

L
x
V

P

P

I

E

G

G

W

Y

S

S

V

L

E

D

Q

E

W

A

V

D

A

R

A

L

Q

R

N

Q

D

D

-

T

P

P

S

E

Q

L

Q

Y

A

V

R

R

L

L

T

-

A

-

D

-

A

A

A

K

H

Q

S

S

C

M

A

K

Q

K

H

H

V

V

Q

E

A

A

M

M

L

L

D

A

F

F

H

Q

A

Q

M

K

G

G

I

S

K

I

T

V

V

F

D

D

Y
|
Y

G

G

N

N

N

N

I

I

R

R

Q

Q

V

V

A

A

F

K

D

D

Q

E

G

G

V

L

K

E

N

N

A

A

F

F

D

D

F

F

P

P

G

G

F

F

V

V

P

P

A

A

Y

Y

I

I

R

R

P

P

L

L

F

F

C

C

E

E

G

G

K

K

G

G

P

P

F

F

R

R

W

W

V

A

A

A

L

L

S

S

G

G

D

D

P

P

E

A

D

D

I

I

Y

Y

K

R

T

T

D

D

A

A

K

L

I

L

K

K

E

E

L

L

F

F

P

P

E

T

N

N

K

K

H

A

V

L

H

H

R

R

W

W

L

I

D

D

M

M

A

A

H

Q

E

E

R

K

I

V

A

T

F

F

Q

Q

G

G

L

L

P

P

A

S

R

R

I

I

C

C

W

W

L

L

G

G

L

Y

G

G

E

E

R

R

H

K

I

K

A

M

G

G

L

L

A

A

F

I

N

N

E

E

M

L

V

V

R

R

N

T

G

G

E

E

L

L

K

K

A

A

P

P

V

V

V

V

I

I

G

G

R

R

D

D

H

H

L

L

D

D

T

C

G

G

S

S

V

V

A

A

S

S

P

P

N

N

R

R

E

E

T

T

E

E

S

A

M

M

K

K

D

D

G

G

S

S

D

D

A

A

V

V

S

G

D

D

W

W

P

A

L

V

L

L

N

N

A

A

L

L

L

V

N

N

T

T

S

A

G

A

G

G

A

A

T

S

W

W

V

V

S

S

L

F

H

H

H

H

G

G

G

G

G
|
G

V

V

G

G

M

M

G

G

Y

Y

S

S

Q

L

H

H

A

A

G

G

M

M

V

V

I

A

V

V

A

A

D

D

G

G

T

S

D

E

A

L

A

A

A

D

K

E

R

R

L

L

A

A

R

R

V

V

L

L

V

T

N

S

D

D

C

P

A

G

S

M

G

G

V

I

M

I

R

R

H

H

A

A

D

D

A

A

G

G

Y

Y

E

E

A

R

A

A

V

V

E

E

C

V

A

A

K

K

R

E

H

Q

N

D

L

I

N

I

L

V

P

P

M

M

I

Q

K

K

GG 49:50 (= GG 58:59) binding NAD(+)
Q127 (= Q136) binding NAD(+)
GMG 173:175 (= GMG 182:184) binding NAD(+)
E193 (= E202) binding NAD(+)
R198 (≠ S207) binding NAD(+)
NA 239:240 (= NA 248:249) binding NAD(+)
QTSAH 260:264 (= QTSAH 273:277) binding NAD(+)
YV 270:271 (≠ YL 283:284) binding NAD(+)
Y319 (= Y334) binding NAD(+)
G489 (= G504) binding NAD(+)

2fknB Crystal structure of urocanase from bacillus subtilis
65% identity, 97% coverage: 16:567/567 of query aligns to 1:546/546 of 2fknB

query
sites
2fknB

T

S

I

I

R

R

A

A

A

N

R

R

G

G

T

T

Q

E

L

L

T

E

A

C

K

L

S

G

W

W

L

E

T

Q

E

E

A

A

P

V

L

L

R

R

M

M

L

L

M

R

N

N

N

N

L

L

D

D

P

P

E

E

V

V

A

A

E

E

R

K

P

P

N

E

D

D

L

L

V

I

V

V

Y
|
Y

G
|
G

G
|
G

I

I

G

G

R

K

A

A

A

A

R

R

D

D

W

W

A

D

C

A

F

F

D

H

K

A

I

I

V

E

E

H

A

S

L

L

T

K

K

T

L

L

N

K

D

N

D

D

E

E

T

T

L

L

L

L

I

V

Q

Q

S

S

G

G

K

K

P

P

V

V

G

G

V

M

F

F

Q

R

T

T

H

H

A

P

D

Q

A

A

P

P

R

R

V

V

L

L

L

L

A

A

N

N

S

S

N

V

L

L

V

V

P

P

K

K

W

W

A

A

N

D

W

W

E

E

H

H

F

F

N

H

E

E

L

L

D

E

R

K

K

K

G

G

L

L

F

M

M

M

Y

Y

G

G

Q

Q

M

M

T

T

A

A

G

G

S

S

W

W

I

I

Y

Y

I

I

G

G

S

S

Q

Q

G

G

I
|
I

V

L

Q

Q

G

G

T

T

F

Y

E

E

T

T

F

F

A

A

E

E

A

L

G

A

R

R

Q

Q

H

H

F

F

G

G

G

G

D

S

L

L

S

K

G

G

R

T

W

L

I

T

L

L

T

T

A

A

G

G

L

L

G
|
G

G
|
G

M
|
M

G

G

G

G

A

A

Q

Q

P

P

L

L

A

S

A

V

T

T

L

M

A

N

G

E

A

G

A

V

S

V

L

I

T

A

I

V

E
|
E

C
x
V

K

D

Q

E

S

K

S
x
R

I

I

D

D

F

K

R

R

L

I

R

E

T

T

R

K

Y

Y

L

C

D

D

K

R

Q

K

A

T

K

A

N

S

L

I

D

E

D

E

A

A

L

L

D

A

L

W

I

A

K

E

Y

E

H

A

T

K

E

L

R

A

K

G

E

K

A

P

I

L

S

S

I

I

G

A

L

L

L

L

G

G

N
|
N

A
|
A

A

A

E

E

V

V

L

H

P

H

E

T

L

L

V

L

R

N

R

R

A

-

K

-

A

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G

-

G

G

I

V

K

K

P

I

D

D

L

I

V

V

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T

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D

Q
|
Q

T
|
T

S
|
S

A

A

H
|
H

D

D

L

P

I

L

N

I

G

G

Y
|
Y

L
x
V

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P

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|
N

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|
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|
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M

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G

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A

A

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W

W

V

V

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S

L

F

H

H

H

H

G

G

G

G

G
|
G

V

V

G

G

M

M

G

G

Y

Y

S

S

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L

H

H

A

A

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G

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M

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V

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L

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D

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A

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S

M

G

G

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I

M

I

R

R

H

H

A

A

D

D

A

A

G

G

Y

Y

E

E

A

R

A

A

V

V

E

E

C

V

A

A

K

K

R

E

H

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N

D

L

I

N

I

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M

M

I

Q

K

K

binding nicotinamide-adenine-dinucleotide: Y42 (= Y57), G43 (= G58), G44 (= G59), I135 (= I150), G166 (= G181), G167 (= G182), M168 (= M183), E187 (= E202), V188 (≠ C203), R192 (≠ S207), N233 (= N248), A234 (= A249), Q254 (= Q273), T255 (= T274), S256 (= S275), H258 (= H277), Y264 (= Y283), V265 (≠ L284), N315 (= N336), F335 (= F356), R445 (= R466), G483 (= G504)

Q5L084 Urocanate hydratase; Urocanase; Imidazolonepropionate hydrolase; EC 4.2.1.49 from Geobacillus kaustophilus (strain HTA426)
65% identity, 98% coverage: 14:567/567 of query aligns to 4:551/551 of Q5L084

query
sites
Q5L084

S

K

R

R

T

T

I

V

R

S

A

P

A

P

R

A

G

G

T

T

Q

E

L

R

T

R

A

A

K

K

S

G

W

W

L

I

T

Q

E

E

A

A

P

A

L

L

R

R

M

M

L

L

M

N

N

N

N

N

L

L

D

H

P

P

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D

V

V

A

A

E

E

R

R

P

P

N

D

D

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L

L

V

I

V

V

Y

Y

G

G

G

G

I

I

G

G

R

K

A

A

A

A

R

R

D

N

W

W

A

E

C

C

F

Y

D

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K

A

I

I

V

V

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D

A

T

L

L

T

L

K

R

L

L

N

E

D

N

D

D

E

E

T

T

L

L

L

L

I

I

Q

Q

S

S

G

G

K

K

P

P

V

V

G

A

V

V

F

F

Q

R

T

T

H

H

A

P

D

D

A

A

P

P

R

R

V

V

L

L

L

I

A

A

N

N

S

S

N

N

L

L

V

V

P

P

K

A

W

W

A

A

N

T

W

W

E

D

H

H

F

F

N

H

E

E

L

L

D

D

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K

K

K

G

G

L

L

F

I

M

M

Y

Y

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M

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W

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I

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L

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A

A

G

G

L

L

G

G

G
|
G

M
|
M

G
|
G

G

G

A

A

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Q

P

P

L

L

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A

A

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|
E

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K

K

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A

A

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S

S

I

I

G

G

L

L

L

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G

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N
|
N

A
|
A

A

A

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E

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V

L

L

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P

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L

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|
Q

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|
T

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A
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A

H
|
H

D

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I

L

N

N

G

G

Y
|
Y

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x
I

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|
Y

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N

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L

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N

R

R

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M

M

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K

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D

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G

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A

A

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D

W

W

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I

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L

N

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A

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G

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W

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H

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V

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M

M

G

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Y

Y

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V

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A

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A

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V

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N

T

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D

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A

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G

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V

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R

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H

A

A

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D

A

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Y

Y

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A

L

A

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C

T

A

A

K

K

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H

K

N

G

L

I

N

D

L

M

P

P

M

M

I

L

K

K

GMG 172:174 (= GMG 182:184) binding NAD(+)
E192 (= E202) binding NAD(+)
R197 (≠ S207) binding NAD(+)
NA 238:239 (= NA 248:249) binding NAD(+)
QTSAH 259:263 (= QTSAH 273:277) binding NAD(+)
YI 269:270 (≠ YL 283:284) binding NAD(+)
Y318 (= Y334) binding NAD(+)

1x87A 2.4a x-ray structure of urocanase protein complexed with NAD
58% identity, 97% coverage: 17:567/567 of query aligns to 1:495/495 of 1x87A

query
sites
1x87A

I

V

R

R

A

P

A

F

R

A

G

G

T

T

Q

E

L

R

T

R

A

A

K

K

S

G

W

W

L

I

T

Q

E

E

A

A

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A

L

L

R

R

M

M

L

L

M

N

N

N

N

N

L

L

D

H

P

P

E

D

V

V

A

-

E

-

R

-

P

-

N

-

D

-

L

-

V

-

V

-

Y

-

G

-

G

-

I

-

G

-

R

K

A

A

A

A

R

R

D

N

W

W

A

E

C

C

F

Y

D

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K

A

I

I

V

V

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D

A

T

L

L

T

L

K

R

L

L

N

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D

N

D

D

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T

L

L

L

L

I

I

Q

Q

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G

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R

V

V

L

L

L

I

A

A

N

N

S

S

N

N

L

L

V

V

P

P

K

A

W

W

A

A

N

T

W

W

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H

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F

-

N

-

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L

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G

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Q

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Y

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A

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G

R

T

W

I

I

T

L

L

T

T

A

A

G

G

L

L

G
|
G

G
|
G

M
|
M

G

G

G

G

A

A

Q

Q

P

P

L

L

A

A

A

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T

T

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M

A

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A

G

A

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C

L

L

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A

I

I

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|
E

C
x
V

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D

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A

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x
R

I

I

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L

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T

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N

Y

Y

L

L

D

D

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M

A

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L

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A

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L

M

I

A

K

K

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A

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E

K

K

E

K

A

A

I

L

S

S

I

I

G

G

L

L

L

V

G

G

N
|
N

A
|
A

A

A

E

E

V

V

L

L

P

P

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R

L

L

V

V

R

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A

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A

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G

-

G

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I

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L

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V

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D

D

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Q

T
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T

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S

A

A

H
|
H

D

D

L

P

I

L

N

N

G

G

Y
|
Y

L
x
I

P

P

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A

G

G

W

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G

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Y
|
Y

G
|
G

N
|
N

N

N

I

I

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R

Q

Q

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V

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A

F

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D

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G

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|
F

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V

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R

I

I

C

C

W

W

L

L

G

G

L

Y

G

G

E

E

R

R

H

A

I

K

A

F

G

G

L

K

A

I

F

I

N

N

E

D

M

M

V

V

R

A

N

K

G

G

E

E

L

L

K

K

A

A

P

P

V

I

V

V

I

I

G

G

R

R

D

D

H

H

L

L

D

-

T

-

G

-

S

-

V

-

A

-

S

-

P

-

N

-

R

-

E

-

T

-

E

-

S

-

M

-

K

-

D

-

G

-

S

-

D

D

A

A

V

I

S

A

D

D

W

W

P

P

L

I

L

L

N

N

A

A

L

L

L

L

N

N

T

A

S

V

G

G

G

G

A

A

T

S

W

W

V

V

S

S

L

V

H

H

H

H

G

G

G

G

G

G

V

V

G

G

M

M

G

G

Y

Y

S

S

Q

I

H

H

A

A

G

G

M

M

V

V

I

I

V

V

A

A

D

D

G

G

T

T

D

K

A

E

A

A

A

E

K

K

R

R

L

L

A

E

R

R

V

V

L

L

V

T

N

T

D

D

C

P

A

G

S

L

G

G

V

V

M

I

R

R

H

H

A

A

D

D

A

A

G

G

Y

Y

E

E

A

L

A

A

V

I

E

R

C

T

A

A

K

K

R

E

H

K

N

G

L

I

N

D

L

M

P

P

M

M

I

L

K

K

binding nicotinamide-adenine-dinucleotide: G134 (= G181), G135 (= G182), M136 (= M183), E155 (= E202), V156 (≠ C203), R160 (≠ S207), N201 (= N248), A202 (= A249), Q222 (= Q273), T223 (= T274), H226 (= H277), Y232 (= Y283), I233 (≠ L284), Y281 (= Y334), G282 (= G335), N283 (= N336), F303 (= F356)

7nedA Thiourocanate hydratase from paenibacillus sp. Soil724d2 in complex with cofactor NAD+ and urocanate (see paper)
50% identity, 96% coverage: 17:561/567 of query aligns to 3:539/545 of 7nedA

query
sites
7nedA

I

I

R

T

A

A

A

A

R

R

G

G

T

L

Q

E

L

L

T

R

A

C

K

K

S

G

W

W

L

R

T

Q

E

E

A

A

P

L

L

L

R

R

M

M

L

L

M

E

N

N

N

V

L

L

D

-

P

-

E

E

V

N

A

G

E

E

R

N

P

Q

N

K

D

E

L

L

V

I

V

V

Y
|
Y

G
x
A

G
x
A

I

L

G

A

R

K

A

A

A

A

R

R

D

N

W

W

A

P

C

S

F

Y

D

H

K

A

I

I

V

V

E

R

A

T

L

L

T

K

K

E

L

L

N

E

D

E

D

D

E

E

T

T

L

L

L

V

I

I

Q

Q

S

S

G

G

K

K

P

P

V

I

G

G

V

I

F

F

Q

K

T

T

H

H

A

R

D

F

A

A

P

P

R

L

V

I

L

V

L

M

A

A

N

N

S

C

N

N

L

L

V

V

P

G

K

R

W

W

A

A

N

T

W

S

E

E

H

N

F

F

N

Y

E

R

L

L

D

Q

R

E

K

K

G

G

L

L

F

L

M

I

Y

W

G

G

Q

G

M
x
L

T
|
T

A

A

G

A

S

A

W

W

I

Q

Y

Y

I

I

G

G

S

S

Q

Q

G

G

I

V

V

I

Q

Q

G

G

T

T

F

Y

E

E

T

I

F

F

A

Q

E

S

A

I

G

A

R

R

Q

L

H

H

F

F

G

N

G

G

D

S

L

L

S

A

G

G

R

K

W

F

I

I

L

L

T

T

A

A

G

G

L

L

G
|
G

G
|
G

M
|
M

G

G

G

G

A

A

Q

Q

P

P

L

L

A

A

A

G

T

T

L

L

A

A

G

G

A

A

A

A

S

I

L

L

T

C

I

V

E
|
E

C
x
V

K

S

Q

E

S

D

S
x
R

I

V

D

D

F

R

R

R

L

L

R

Q

T

T

R

N

Y

Y

L

L

D

Q

K

R

Q

K

A

T

K

R

N

S

L

L

D

D

D

E

A

A

L

L

D

L

L

W

I

I

K

E

Y

E

H

A

T

V

E

D

R

N

K

L

E

H

A

P

I

V

S

S

I

V

G

G

L

L

L

T

G

G

N
|
N

A
|
A

A

S

E

D

V

I

L

Y

P

P

E

E

L

L

V

V

R

R

R

R

A

-

K

-

A

-

G

-

G

G

I

I

K

T

P

P

D

D

L

I

V

V

T

T

D

D

Q
|
Q

T
|
T

S

S

A

A

H
|
H

D

D

L

L

I

V

N

Y

G

G

Y
|
Y

L
x
V

P

P

I

S

G

G

W

Y

S

R

V

V

E

E

Q

E

W

L

V

E

A

E

A

A

Q

R

-

A

N

N

D

D

P

P

S

E

Q

Q

Q

L

A

Q

R

R

L

-

T

-

A

-

D

D

A

A

A

G

H

A

S

S

C

I

A

A

Q

V

H

E

V

V

Q

E

A

A

M

M

L

L

D

E

F

L

H

K

A

K

M

R

G

G

I

A

K

I

T

V

V

F

D

D

Y

N

G
|
G

N
|
N

N

N

I

I

R

R

Q

S

V

Q

A

A

F

K

D

E

Q

Y

G

G

V

V

K

Q

N

N

A

A

F

F

D

D

F

I

P

D

G

I

F

F

V

T

P

E

A

A

Y

F

I

L

R

R

P

P

L

L

F

F

C

A

E

R

G

A

K

I

G

G

P

P

F

F

R
|
R

W

W

V

V

A

A

L

L

S

S

G

G

D

E

P

L

E

S

D

D

I

I

Y

H

K

A

T

I

D

D

A

E

K

F

I

I

K

L

E

E

L

A

F

F

P

S

E

D

N

N

K

E

H

V

V

I

H

A

R

N

W

W

L

I

D

R

M

L

A

A

H

R

E

E

R

H

I

V

A

P

F

V

Q

E

G

G

L

L

P

P

A

A

R

R

I

I

C

G

W

W

L

F

G

G

L

H

G

G

E

D

R

R

H

T

I

K

A

L

G

A

L

L

A

A

F

V

N

N

E

Q

M

M

V

V

R

R

N

E

G

G

E

K

L

L

K

Q

A

G

P

P

V

I

V

A

I

F

G

S

R

R

D
|
D

H

H

L

L

D

D

T

A

G

A

S

S

V

M

A

T

S

H

P

P

N

N

R
x
I

E

M

T

T

E

E

S

R

M

M

K

K

D

D

G

G

S

S

D

D

A

A

V

I

S

A

D

D

W

W

P

P

L

L

L

L

N

N

A

A

L

M

L

L

N

N

T

C

S

S

G

S

G

M

A

A

T

D

W

L

V

V

S

T

L

I

H

H

H

S

G

G

G

G

G

G

V

G

G
x
Y

M

A

G

G

Y

Y

S

M

Q

T

H

S

A

A

G

G

M

V

V

T

I

L

V

V

A

A

D

D

G

G

T

S

D

T

A

E

A

S

A

D

K

I

R

R

L

L

A

E

R

T

V

T

L

L

V

N

N

N

D

D

C

T

A

G

S

L

G

G

V

V

M

L

R

R

H

Y

A

A

D

D

A

A

G

G

Y

Y

E

E

A

E

A

S

V

A

E

D

C

E

A

V

K

R

R

L

H

K

N

D

L

I

binding nicotinamide-adenine-dinucleotide: Y41 (= Y57), A42 (≠ G58), A43 (≠ G59), G165 (= G181), G166 (= G182), M167 (= M183), E186 (= E202), V187 (≠ C203), R191 (≠ S207), N232 (= N248), A233 (= A249), Q253 (= Q273), T254 (= T274), H257 (= H277), Y263 (= Y283), V264 (≠ L284), G313 (= G335), N314 (= N336), I444 (≠ R466), Y484 (≠ G506)
binding (2e)-3-(1h-imidazol-4-yl)acrylic acid: Y41 (= Y57), L121 (≠ M137), T122 (= T138), M167 (= M183), R351 (= R373), D432 (= D454)

8q9vA S-methylthiourocanate hydratase from variovorax sp. Ra8 in complex with NAD+ and imidazolone propionate (see paper)
51% identity, 97% coverage: 15:565/567 of query aligns to 3:540/543 of 8q9vA

query
sites
8q9vA

R

R

T

N

I

I

R

K

A

A

A

A

R

R

G

G

T

T

Q

T

L

L

T

R

A

C

K

K

S

G

W

W

L

Q

T

Q

E

E

A

T

P

I

L

L

R

R

M

L

L

L

M

E

N

N

N

N

L

I

D

-

P

-