SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_061533629.1 NCBI__GCF_001584165.1:WP_061533629.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
38% identity, 38% coverage: 393:640/661 of query aligns to 4:253/253 of 1g9xB

query
sites
1g9xB

L

I

L

L

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R

G

T

K

E

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N

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Y

F
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G

G

E

L
x
F

K

K

A

A

L

L

N

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E

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N

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L

I

D

S

I

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C

K

K

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I

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G

P

P

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N

G
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G

S
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S

G
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G

K
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K

S
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S

T
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T

M

L

M

I

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N

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V

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T

G

G

I

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L

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K

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H

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Y

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G

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I

A

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R

T

T

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F

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L

P

F

L

G

K

E

E

M

M

T

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A

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L

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L

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G

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M

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L

L

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E

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P

A

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A

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G

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F

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G

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K

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A

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G

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A

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G

binding adenosine-5'-diphosphate: F14 (= F403), F17 (≠ L406), G40 (= G429), S41 (= S430), G42 (= G431), K43 (= K432), S44 (= S433), T45 (= T434)
binding magnesium ion: S44 (= S433), E176 (= E567)

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
38% identity, 38% coverage: 393:640/661 of query aligns to 4:253/254 of 1g6hA

query
sites
1g6hA

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A

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G

A

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A

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E

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Y

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I

G

G

binding adenosine-5'-diphosphate: F14 (= F403), F17 (≠ L406), N39 (= N428), G40 (= G429), G42 (= G431), K43 (= K432), S44 (= S433), T45 (= T434)

1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
29% identity, 36% coverage: 403:640/661 of query aligns to 16:238/240 of 1ji0A

query
sites
1ji0A

F
x
Y

G

G

G

A

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H

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N

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G

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A

N

N

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S

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x
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M

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F

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A

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M

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E

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F

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M

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T

A

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Y

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E

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N

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M

G

G

L

A

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Y

H

N

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-

F

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R

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S

-

N

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M

-

L

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D

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V

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V

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F

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N

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T

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P

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L

L

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M

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D

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E

P

P

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G

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K

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N

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G

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T

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I

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L

L

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E

H

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H

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M

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D

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N

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G

binding adenosine-5'-triphosphate: Y16 (≠ F403), G42 (= G429), G44 (= G431), K45 (= K432), T46 (≠ S433), T47 (= T434), E88 (≠ Q475), E165 (= E567)

P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
31% identity, 38% coverage: 393:640/661 of query aligns to 4:239/501 of P04983

query
sites
P04983

L

L

L

L

S

Q

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L

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K

Q

G

I

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D

M

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Q

A

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A

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A

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N

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Y

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P

G

G

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M

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A

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E

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N

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A

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N

E

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L

Q

N

L

L

F

I

G

P

E

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M

L

T

T

A

I

T

A

E

E

N

N

V

I

L

F

V

L

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G

L

R

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E

H

F

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V

F

N

R

R

S

F

N

G

M

K

L

I

D

D

V

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V

-

F

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K

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D

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F

I

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A

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E

A

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A

A

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M

L

V

G

G

K43 (= K432) mutation to R: Loss of transport.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
31% identity, 34% coverage: 402:628/661 of query aligns to 10:223/240 of 4ymuJ

query
sites
4ymuJ

Q

N

F
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F

G

G

G

S

L

L

K

E

A
x
V

L

L

N

K

E

G

V

V

N

T

L

L

D

K

I

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V

N

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K

G

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L

I

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I

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G

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P

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x
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G
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G

S
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S

G
|
G

K
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K

S
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S

T
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T

M

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L

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A

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T

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E

E

N

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M

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G

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M

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H

H

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M

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G

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P

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V

I

binding adenosine-5'-triphosphate: F11 (= F403), V16 (≠ A408), S36 (≠ N428), G37 (= G429), S38 (= S430), G39 (= G431), K40 (= K432), S41 (= S433), T42 (= T434), E162 (= E567), H194 (= H599)
binding magnesium ion: S41 (= S433), E162 (= E567)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
29% identity, 35% coverage: 403:633/661 of query aligns to 12:228/241 of 4u00A

query
sites
4u00A

F
|
F

G

G

G

P

L

L

K

H

A
x
V

L

L

N

K

E

G

V

I

N

H

L

L

D

E

I

V

V

A

K

P

G

G

S

E

V

K

H

L

G

V

L

I

I

I

G

G

P

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

M

L

M

I

N

R

V

T

L

I

T

N

G

R

I

L

Y

E

K

D

P

F

T

Q

S

E

G

G

K

E

V

V

E

V

F

V

N

D

G

G

R

L

T

S

I

V

S

K

G

D

T

D

T

R

P

A

S

L

A

R

I

E

A

I

L

R

G

R

G

E

V

V

A

G

R

M

T

V

F

F

Q

Q

N

Q

V

F

Q

N

L

L

F

F

G

P

E

H

M

M

T

T

A

V

T

L

E

E

N

N

V

V

L

T

V

L

G

A

L

-

H

-

H

-

T

-

F

-

R

-

S

-

N

-

M

-

L

-

D

-

V

-

V

P

F

M

N

R

T

V

P

R

R

R

Y

W

R

P

R

R

E

E

E

-

Q

-

S

K

A

A

R

E

E

K

R

K

A

A

A

L

S

E

I

L

L

L

Q

E

F

R

V

V

G

G

L

I

T

L

D

D

L

Q

A

A

T

R

E

K

E

Y

A

P

R

A

N

Q

L

L

P

S

Y

G

G

G

K

Q

Q

Q

R

Q

L

R

L

V

E

A

I

I

G

A

R

R

A

A

L

L

G

A

L

M

N

E

P

P

E

K

L

I

L

M

L

L

L

F

D

D

E

E

P

P

A

T

A

S

G

A

L

L

T

D

A

P

P

E

D

M

I

V

K

G

E

E

L

V

I

L

A

D

I

V

I

M

H

R

K

D

I

L

R

A

Q

Q

H

G

G

G

I

M

T

T

I

M

I

V

L

V

I

V

E

T

H

H

H

E

M

M

D

G

V

F

V

A

M

R

S

E

I

V

S

A

D

D

T

R

V

V

T

V

V

F

L

M

D

D

F

G

G

G

Q

Q

K

I

I

V

A

E

E

E

G

G

R

R

P

P

A

E

A

E

V

I

Q

F

V

T

D

R

P

P

K

K

binding adenosine-5'-diphosphate: F12 (= F403), V17 (≠ A408), S37 (≠ N428), G38 (= G429), S39 (= S430), G40 (= G431), K41 (= K432), S42 (= S433), T43 (= T434)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
32% identity, 34% coverage: 406:633/661 of query aligns to 18:233/343 of P30750

query
sites
P30750

L

I

K

Q

A

A

L

L

N

N

E

N

V

V

N

S

L

L

D

H

I

V

V

P

K

A

G

G

S

Q

V

I

H

Y

G

G

L

V

I

I

G

G

P

A

N
x
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

M

L

M

I

N

R

V

C

L

V

T

N

G

L

I

L

Y

E

K

R

P

P

T

T

S

E

G

G

K

S

V

V

E

L

F

V

N

D

G

G

R

Q

T

E

I

L

S

T

G

T

T

L

T

S

P

E

S

S

A

E

I

L

-

T

-

K

A

A

L

R

G

R

G

Q

V

I

A

G

R

M

T

I

F

F

Q

Q

N

H

V

F

Q

N

L

L

F

L

G

S

E

S

M

R

T

T

A

V

T

F

E

G

N

N

V

V

L

A

V

L

G

P

L

L

H

E

H

-

T

-

F

-

R

-

S

-

N

-

M

-

L

L

D

D

V

-

V

-

F

-

N

N

T

T

P

P

R

K

Y

-

R

-

R

-

E

-

E

-

Q

D

S

E

A

V

R

K

E

R

R

R

A

V

A

T

S

E

I

L

L

L

Q

S

F

L

V

V

G

G

L

L

T

G

D

D

L

K

A

H

T

D

E

S

E

Y

A

P

R

S

N

N

L

L

P

S

Y

G

G

G

K

Q

Q

K

R

Q

L

R

L

V

E

A

I

I

G

A

R

R

A

A

L

L

G

A

L

S

N

N

P

P

E

K

L

V

L

L

L

L

L

C

D

D

E
|
E

P

A

A

T

A

S

G

A

L

L

T

D

A

P

P

A

D

T

I

T

K

R

E

S

L

I

I

L

A

E

I

L

I

L

H

K

K

D

I

I

-

N

R

R

Q

R

H

L

G

G

I

L

T

T

I

I

I

L

L

L

I

I

E

T

H

H

H

E

M

M

D

D

V

V

V

V

M

K

S

R

I

I

S

C

D

D

T

C

V

V

T

A

V

V

L

I

D

S

F

N

G

G

Q

E

K

L

I

I

A

E

E

Q

G

D

R

T

P

V

A

S

A

E

V

V

Q

F

V

S

D

H

P

P

K

K

40:46 (vs. 428:434, 71% identical) binding ATP
E166 (= E567) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

Q5M244 Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; EC 3.6.3.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
32% identity, 34% coverage: 407:628/661 of query aligns to 21:232/280 of Q5M244

query
sites
Q5M244

K

R

A

A

L

L

N

F

E

D

V

M

N

T

L

V

D

T

I

I

V

K

K

D

G

G

S

S

V

Y

H

T

G

A

L

F

I

I

G

G

P

H

N

T

G

G

S

S

G

G

K

K

S

S

T

T

M

I

M

M

N

Q

V

L

L

L

T

N

G

G

I

L

Y

Y

K

L

P

P

T

T

S

S

G

G

K

Q

V

V

E

K

F

V

N

D

G

D

R

T

T

I

I

I

S

N

G

S

T

Q

T

S

P

K

S

N

A

K

I

E

-

I

-

K

-

P

-

I

-

R

A

K

L

K

G

V

G

G

V

L

A

V

R

F

T

Q

F

F

Q

P

N

E

V

S

Q
|
Q

L

L

F

F

G

A

E

E

M

T

T

V

A

-

T

-

E

-

N

-

V

-

L

-

V

-

G

-

L

-

H

-

H

-

T

-

F

-

R

-

S

-

N

-

M

L

L

E

D

D

V

I

V

A

F

F

N

G

T

P

P

Q

R

N

Y

F

R

G

R

V

E

S

E

K

Q

E

S

E

A

A

R

E

E

Q

R

R

A

A

A

L

S

E

I

S

L

L

Q

R

F

L

V

V

G

G

L

L

T

S

D

D

-

E

L

L

A

R

T

D

E

Q

E

N

A

P

R

F

N

D

L

L

P

S

Y

G

G

G

K

Q

Q

M

R

R

L

R

L

V

E

A

I

I

G

A

R

G

A

I

L

L

G

A

L

M

N

Q

P

P

E

D

L

I

L

L

L

V

L

L

D

D

E
|
E

P

P

A

T

A

A

G

G

L

L

T

D

A

P

P

Q

D

G

I

R

K

K

E

E

L

L

I

M

A

S

I

L

I

F

H

K

K

Q

I

L

R

H

Q

L

H

S

G

G

I

I

T

T

I

I

I

V

L

L

I

V

E

T

H

H

H

L

M

M

D

D

V

D

V

V

M

A

S

D

I

Y

S

A

D

T

T

A

V

V

T

N

V

V

L

M

D

E

F

K

G

G

Q

R

K

L

I

V

A

L

E

S

G

G

R

T

P

P

A

K

A

D

V

V

Q97 (= Q478) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-90 in EcfA1.
E171 (= E567) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-163 in EcfA1.

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
32% identity, 34% coverage: 406:633/661 of query aligns to 19:234/344 of 6cvlD

query
sites
6cvlD

L
x
I

K

Q

A

A

L

L

N

N

E

N

V

V

N

S

L

L

D

H

I

V

V

P

K

A

G

G

S

Q

V

I

H

Y

G

G

L

V

I

I

G

G

P

A

N
x
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

M

L

M

I

N

R

V

C

L

V

T

N

G

L

I

L

Y

E

K

R

P

P

T

T

S

E

G

G

K

S

V

V

E

L

F

V

N

D

G

G

R

Q

T

E

I

L

S

T

G

T

T

L

T

S

P

E

S

S

A

E

I

L

-

T

-

K

A

A

L

R

G

R

G

Q

V

I

A

G

R

M

T

I

F

F

Q

Q

N

H

V

F

Q

N

L

L

F

L

G

S

E

S

M

R

T

T

A

V

T

F

E

G

N

N

V

V

L

A

V

L

G

P

L

L

H

E

H

-

T

-

F

-

R

-

S

-

N

-

M

-

L

L

D

D

V

-

V

-

F

-

N

N

T

T

P

P

R

K

Y

-

R

-

R

-

E

-

E

-

Q

D

S

E

A

V

R

K

E

R

R

R

A

V

A

T

S

E

I

L

L

L

Q

S

F

L

V

V

G

G

L

L

T

G

D

D

L

K

A

H

T

D

E

S

E

Y

A

P

R

S

N
|
N

L

L

P
x
S

Y

G

G

G

K
x
Q

Q

K

R

Q

L

R

L

V

E

A

I

I

G

A

R

R

A

A

L

L

G

A

L

S

N

N

P

P

E

K

L

V

L

L

L

L

L

C

D

D

E

Q

P

A

A

T

A

S

G

A

L

L

T

D

A

P

P

A

D

T

I

T

K

R

E

S

L

I

I

L

A

E

I

L

I

L

H

K

K

D

I

I

-

N

R

R

Q

R

H

L

G

G

I

L

T

T

I

I

I

L

L

L

I

I

E

T

H
|
H

H

E

M

M

D

D

V

V

V

V

M

K

S

R

I

I

S

C

D

D

T

C

V

V

T

A

V

V

L

I

D

S

F

N

G

G

Q

E

K

L

I

I

A

E

E

Q

G

D

R

T

P

V

A

S

A

E

V

V

Q

F

V

S

D

H

P

P

K

K

binding phosphothiophosphoric acid-adenylate ester: I19 (≠ L406), S41 (≠ N428), G42 (= G429), A43 (≠ S430), G44 (= G431), K45 (= K432), S46 (= S433), T47 (= T434), N141 (= N541), S143 (≠ P543), Q146 (≠ K546), H200 (= H599)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
32% identity, 34% coverage: 406:633/661 of query aligns to 19:234/344 of 3tuzC

query
sites
3tuzC

L

I

K

Q

A

A

L

L

N

N

E

N

V

V

N

S

L

L

D

H

I

V

V

P

K

A

G

G

S

Q

V

I

H

Y

G

G

L

V

I

I

G

G

P

A

N

S

G
|
G

S

A

G
|
G

K
|
K

S
|
S

T
|
T

M

L

M

I

N

R

V

C

L

V

T

N

G

L

I

L

Y

E

K

R

P

P

T

T

S

E

G

G

K

S

V

V

E

L

F

V

N

D

G

G

R

Q

T

E

I

L

S

T

G

T

T

L

T

S

P

E

S

S

A

E

I

L

-

T

-

K

A

A

L

R

G

R

G

Q

V

I

A

G

R

M

T

I

F

F

Q

Q

N

H

V

F

Q

N

L

L

F

L

G

S

E

S

M

R

T

T

A

V

T

F

E

G

N

N

V

V

L

A

V

L

G

P

L

L

H

E

H

-

T

-

F

-

R

-

S

-

N

-

M

-

L

L

D

D

V

-

V

-

F

-

N

N

T

T

P

P

R

K

Y

-

R

-

R

-

E

-

E

-

Q

D

S

E

A

V

R

K

E

R

R

R

A

V

A

T

S

E

I

L

L

L

Q

S

F

L

V

V

G

G

L

L

T

G

D

D

L

K

A

H

T

D

E

S

E

Y

A

P

R

S

N

N

L

L

P

S

Y

G

G

G

K

Q

Q

K

R

Q

L

R

L

V

E

A

I

I

G

A

R

R

A

A

L

L

G

A

L

S

N

N

P

P

E

K

L

V

L

L

L

L

L

C

D

D

E

Q

P

A

A

T

A

S

G

A

L

L

T

D

A

P

P

A

D

T

I

T

K

R

E

S

L

I

I

L

A

E

I

L

I

L

H

K

K

D

I

I

-

N

R

R

Q

R

H

L

G

G

I

L

T

T

I

I

I

L

L

L

I

I

E

T

H

H

H

E

M

M

D

D

V

V

V

V

M

K

S

R

I

I

S

C

D

D

T

C

V

V

T

A

V

V

L

I

D

S

F

N

G

G

Q

E

K

L

I

I

A

E

E

Q

G

D

R

T

P

V

A

S

A

E

V

V

Q

F

V

S

D

H

P

P

K

K

binding adenosine-5'-diphosphate: G42 (= G429), G44 (= G431), K45 (= K432), S46 (= S433), T47 (= T434)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
32% identity, 34% coverage: 406:633/661 of query aligns to 19:234/344 of 3tuiC

query
sites
3tuiC

L

I

K

Q

A

A

L

L

N

N

E

N

V

V

N

S

L

L

D

H

I

V

V

P

K

A

G

G

S

Q

V

I

H

Y

G

G

L

V

I

I

G

G

P

A

N

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

M

L

M

I

N

R

V

C

L

V

T

N

G

L

I

L

Y

E

K

R

P

P

T

T

S

E

G

G

K

S

V

V

E

L

F

V

N

D

G

G

R

Q

T

E

I

L

S

T

G

T

T

L

T

S

P

E

S

S

A

E

I

L

-

T

-

K

A

A

L

R

G

R

G

Q

V

I

A

G

R

M

T

I

F

F

Q

Q

N

H

V

F

Q

N

L

L

F

L

G

S

E

S

M

R

T

T

A

V

T

F

E

G

N

N

V

V

L

A

V

L

G

P

L

L

H

E

H

-

T

-

F

-

R

-

S

-

N

-

M

-

L

L

D

D

V

-

V

-

F

-

N

N

T

T

P

P

R

K

Y

-

R

-

R

-

E

-

E

-

Q

D

S

E

A

V

R

K

E

R

R

R

A

V

A

T

S

E

I

L

L

L

Q

S

F

L

V

V

G

G

L

L

T

G

D

D

L

K

A

H

T

D

E

S

E

Y

A

P

R

S

N

N

L

L

P

S

Y

G

G

G

K

Q

Q

K

R

Q

L

R

L

V

E

A

I

I

G

A

R

R

A

A

L

L

G

A

L

S

N

N

P

P

E

K

L

V

L

L

L

L

L

C

D

D

E

Q

P

A

A

T

A

S

G

A

L

L

T

D

A

P

P

A

D

T

I

T

K

R

E

S

L

I

I

L

A

E

I

L

I

L

H

K

K

D

I

I

-

N

R

R

Q

R

H

L

G

G

I

L

T

T

I

I

I

L

L

L

I

I

E

T

H

H

H

E

M

M

D

D

V

V

V

V

M

K

S

R

I

I

S

C

D

D

T

C

V

V

T

A

V

V

L

I

D

S

F

N

G

G

Q

E

K

L

I

I

A

E

E

Q

G

D

R

T

P

V

A

S

A

E

V

V

Q

F

V

S

D

H

P

P

K

K

binding adenosine-5'-diphosphate: G42 (= G429), A43 (≠ S430), G44 (= G431), K45 (= K432), S46 (= S433), T47 (= T434)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

5x40A Structure of a cbio dimer bound with amppcp (see paper)
30% identity, 36% coverage: 391:631/661 of query aligns to 2:230/280 of 5x40A

query
sites
5x40A

T

T

T

P

L

I

L

L

S

A

G

A

K

E

Q

A

I

L

L

T

M

Y

Q

A

F
|
F

-

P

G

G

G

G

L
x
V

K

K

A
|
A

L

L

N

D

E

D

V

L

N

S

L

L

D

A

I

V

V

P

K

K

G

G

S

E

V

S

H

L

G

A

L

I

I

L

G

G

P

P

N
|
N

G
|
G

S

A

G
|
G

K
|
K

S
|
S

T
|
T

M

L

M

L

N

L

V

H

L

L

T

N

G

G

I

T

Y

L

K

R

P

P

T

Q

S

S

G

G

K

R

V

V

E

-

F

-

N

-

G

-

R

-

T

-

I

-

S

-

G

-

T

-

T

-

P

-

S

-

A

-

I

-

A

L

L

L

G

G

G

G

V

T

A

A

R

T

T

G

F

H

Q

S

N

R

V

K

Q

D

L

L

F

T

G

G

E

W

M

R

T

R

A

R

T

V

E

G

N

L

V

V

L

L

V
x
Q

G

D

L

A

H

D

H

D

T

Q

F

L

R

F

S

A

N

T

M

T

L

V

-

F

-

E

D

D

V

V

V

S

F

F

N

G

T

P

P

L

R

N

Y

L

R

G

R

L

E

S

E

E

Q

A

S

E

A

A

R

R

E

A

R

R

A

V

A

E

S

E

I

A

L

L

Q

A

F

A

V

L

G

S

L

I

T

S

D

D

L

L

A

R

T

D

E

R

E

P

A

T

R
x
H

N
x
M

L
|
L

P
x
S

Y

G

G
|
G

K
x
Q

Q

K

R

R

L

R

L

V

E

A

I

I

G

A

R

G

A

A

L

V

G

A

L

M

N

R

P

P

E

E

L

V

L

L

L

L

L

L

D

D

E
x
Q

P

P

A

T

A

A

G

G

L

L

T

D

A

L

P

A

D

G

I

T

K

E

E

Q

L

L

I

L

A

T

I

L

I

L

H

R

K

G

I

L

R

R

Q

A

H

A

G

G

I

M

T

T

I

L

I

V

L

F

I

S

E

T

H
|
H

H

D

M

V

D

E

V

L

V

A

M

A

S

A

I

L

S

A

D

D

T

R

V

V

T

A

V

L

L

F

D

R

F

T

G

G

Q

R

K

V

I

L

A

A

E

E

G

G

R

A

P

A

A

E

A

A

V

V

Q

L

V

S

D

D

binding phosphomethylphosphonic acid adenylate ester: F14 (= F403), V18 (≠ L406), A20 (= A408), N40 (= N428), G41 (= G429), G43 (= G431), K44 (= K432), S45 (= S433), T46 (= T434), Q88 (≠ V491), H139 (≠ R540), M140 (≠ N541), L141 (= L542), S142 (≠ P543), G144 (= G545), Q145 (≠ K546), Q166 (≠ E567), H198 (= H599)
binding magnesium ion: S45 (= S433), Q88 (≠ V491)

3c4jA Abc protein artp in complex with atp-gamma-s
28% identity, 34% coverage: 398:625/661 of query aligns to 8:222/242 of 3c4jA

query
sites
3c4jA

Q

Q

I

L

L

K

M

K

Q

S

F
|
F

G

G

G

S

L

L

K

E

A
x
V

L

L

N

K

E

G

V

I

N

N

L

V

D

H

I

I

V

R

K

E

G

G

S

E

V

V

H

V

G

V

L

V

I

I

G

G

P

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

M

F

M

L

N

R

V

C

L

L

T

N

G

L

I

L

Y

E

K

D

P

F

T

D

S

E

G

G

K

E

V

I

E

I

F

I

N

D

G

G

R

I

T

N

I

L

S

K

G

A

T

K

T

D

P

T

S

N

A

L

I

N

A

K

L

V

-

R

G

E

G

E

V

V

A

G

R

M

T

V

F

F

Q

Q

N

R

V

F

Q

N

L

L

F

F

G

P

E

H

M

M

T

T

A

V

T

L

E

N

N

N

V

I

L

T

V

L

G

A

L

-

H

-

H

-

T

-

F

-

R

-

S

-

N

-

M

-

L

-

D

-

V

-

V

P

F

M

N

K

T

V

P

R

R

K

Y

W

R

P

R

R

E

E

E

K

Q

A

S

E

A

A

R

K

E

-

R

-

A

A

A

M

S

E

I

L

L

L

Q

D

F

K

V

V

G

G

L

L

T

K

D

D

L

K

A

A

T

H

E

A

E

Y

A

P

R

D

N

S

L

L

P

S

Y

G

G

G

K

Q

Q

A

R

Q

L

R

L

V

E

A

I

I

G

A

R

R

A

A

L

L

G

A

L

M

N

E

P

P

E

K

L

I

L

M

L

L

L

F

D

D

E

E

P

P

A

T

A

S

G

A

L

L

T

D

A

P

P

E

D

M

I

V

K

G

E

E

L

V

I

L

A

S

I

V

I

M

H

K

K

Q

I

L

R

A

Q

N

H

E

G

G

I

M

T

T

I

M

I

V

L

V

I

V

E

T

H

H

H

E

M

M

D

G

V

F

V

A

M

R

S

E

I

V

S

G

D

D

T

R

V

V

T

L

V

F

L

M

D

D

F

G

G

G

Q

Y

K

I

I

I

A

E

E

E

G

G

R

K

P

P

binding phosphothiophosphoric acid-adenylate ester: F13 (= F403), V18 (≠ A408), S38 (≠ N428), G39 (= G429), S40 (= S430), G41 (= G431), K42 (= K432), S43 (= S433), T44 (= T434)

3c41J Abc protein artp in complex with amp-pnp/mg2+
28% identity, 34% coverage: 398:625/661 of query aligns to 8:222/242 of 3c41J

query
sites
3c41J

Q

Q

I

L

L

K

M

K

Q

S

F
|
F

G

G

G

S

L

L

K

E

A
x
V

L

L

N

K

E

G

V

I

N

N

L

V

D

H

I

I

V

R

K

E

G

G

S

E

V

V

H

V

G

V

L

V

I

I

G

G

P

P

N
x
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

M

F

M

L

N

R

V

C

L

L

T

N

G

L

I

L

Y

E

K

D

P

F

T

D

S

E

G

G

K

E

V

I

E

I

F

I

N

D

G

G

R

I

T

N

I

L

S

K

G

A

T

K

T

D

P

T

S

N

A

L

I

N

A

K

L

V

-

R

G

E

G

E

V

V

A

G

R

M

T

V

F

F

Q

Q

N

R

V

F

Q

N

L

L

F

F

G

P

E

H

M

M

T

T

A

V

T

L

E

N

N

N

V

I

L

T

V

L

G

A

L

-

H

-

H

-

T

-

F

-

R

-

S

-

N

-

M

-

L

-

D

-

V

-

V

P

F

M

N

K

T

V

P

R

R

K

Y

W

R

P

R

R

E

E

E

K

Q

A

S

E

A

A

R

K

E

-

R

-

A

A

A

M

S

E

I

L

L

L

Q

D

F

K

V

V

G

G

L

L

T

K

D

D

L

K

A

A

T

H

E

A

E

Y

A

P

R

D

N

S

L

L

P

S

Y

G

G

G

K

Q

Q

A

R

Q

L

R

L

V

E

A

I

I

G

A

R

R

A

A

L

L

G

A

L

M

N

E

P

P

E

K

L

I

L

M

L

L

L

F

D
|
D

E

E

P

P

A

T

A

S

G

A

L

L

T

D

A

P

P

E

D

M

I

V

K

G

E

E

L

V

I

L

A

S

I

V

I

M

H

K

K

Q

I

L

R

A

Q

N

H

E

G

G

I

M

T

T

I

M

I

V

L

V

I

V

E

T

H

H

H

E

M

M

D

G

V

F

V

A

M

R

S

E

I

V

S

G

D

D

T

R

V

V

T

L

V

F

L

M

D

D

F

G

G

G

Q

Y

K

I

I

I

A

E

E

E

G

G

R

K

P

P

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F403), V18 (≠ A408), S38 (≠ N428), G39 (= G429), G41 (= G431), K42 (= K432), S43 (= S433), T44 (= T434)
binding magnesium ion: S43 (= S433), D163 (= D566)

2olkA Abc protein artp in complex with adp-beta-s
28% identity, 34% coverage: 398:625/661 of query aligns to 8:222/242 of 2olkA

query
sites
2olkA

Q

Q

I

L

L

K

M

K

Q

S

F
|
F

G

G

G

S

L

L

K

E

A
x
V

L

L

N

K

E

G

V

I

N

N

L

V

D

H

I

I

V

R

K

E

G

G

S

E

V

V

H

V

G

V

L

V

I

I

G

G

P

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

M

F

M

L

N

R

V

C

L

L

T

N

G

L

I

L

Y

E

K

D

P

F

T

D

S

E

G

G

K

E

V

I

E

I

F

I

N

D

G

G

R

I

T

N

I

L

S

K

G

A

T

K

T

D

P

T

S

N

A

L

I

N

A

K

L

V

-

R

G

E

G

E

V

V

A

G

R

M

T

V

F

F

Q

Q

N

R

V

F

Q

N

L

L

F

F

G

P

E

H

M

M

T

T

A

V

T

L

E

N

N

N

V

I

L

T

V

L

G

A

L

-

H

-

H

-

T

-

F

-

R

-

S

-

N

-

M

-

L

-

D

-

V

-

V

P

F

M

N

K

T

V

P

R

R

K

Y

W

R

P

R

R

E

E

E

K

Q

A

S

E

A

A

R

K

E

-

R

-

A

A

A

M

S

E

I

L

L

L

Q

D

F

K

V

V

G

G

L

L

T

K

D

D

L

K

A

A

T

H

E

A

E

Y

A

P

R

D

N

S

L

L

P

S

Y

G

G

G

K

Q

Q

A

R

Q

L

R

L

V

E

A

I

I

G

A

R

R

A

A

L

L

G

A

L

M

N

E

P

P

E

K

L

I

L

M

L

L

L

F

D

D

E

E

P

P

A

T

A

S

G

A

L

L

T

D

A

P

P

E

D

M

I

V

K

G

E

E

L

V

I

L

A

S

I

V

I

M

H

K

K

Q

I

L

R

A

Q

N

H

E

G

G

I

M

T

T

I

M

I

V

L

V

I

V

E

T

H

H

H

E

M

M

D

G

V

F

V

A

M

R

S

E

I

V

S

G

D

D

T

R

V

V

T

L

V

F

L

M

D

D

F

G

G

G

Q

Y

K

I

I

I

A

E

E

E

G

G

R

K

P

P

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F403), V18 (≠ A408), S38 (≠ N428), G39 (= G429), S40 (= S430), G41 (= G431), K42 (= K432), S43 (= S433), T44 (= T434)

2oljA Abc protein artp in complex with adp/mg2+
28% identity, 34% coverage: 398:625/661 of query aligns to 8:222/242 of 2oljA

query
sites
2oljA

Q

Q

I

L

L

K

M

K

Q

S

F
|
F

G

G

G

S

L

L

K

E

A
x
V

L

L

N

K

E

G

V

I

N

N

L

V

D

H

I

I

V

R

K

E

G

G

S

E

V

V

H

V

G

V

L

V

I

I

G

G

P

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

M

F

M

L

N

R

V

C

L

L

T

N

G

L

I

L

Y

E

K

D

P

F

T

D

S

E

G

G

K

E

V

I

E

I

F

I

N

D

G

G

R

I

T

N

I

L

S

K

G

A

T

K

T

D

P

T

S

N

A

L

I

N

A

K

L

V

-

R

G

E

G

E

V

V

A

G

R

M

T

V

F

F

Q

Q

N

R

V

F

Q

N

L

L

F

F

G

P

E

H

M

M

T

T

A

V

T

L

E

N

N

N

V

I

L

T

V

L

G

A

L

-

H

-

H

-

T

-

F

-

R

-

S

-

N

-

M

-

L

-

D

-

V

-

V

P

F

M

N

K

T

V

P

R

R

K

Y

W

R

P

R

R

E

E

E

K

Q

A

S

E

A

A

R

K

E

-

R

-

A

A

A

M

S

E

I

L

L

L

Q

D

F

K

V

V

G

G

L

L

T

K

D

D

L

K

A

A

T

H

E

A

E

Y

A

P

R

D

N

S

L

L

P

S

Y

G

G

G

K

Q

Q

A

R

Q

L

R

L

V

E

A

I

I

G

A

R

R

A

A

L

L

G

A

L

M

N

E

P

P

E

K

L

I

L

M

L

L

L

F

D

D

E

E

P

P

A

T

A

S

G

A

L

L

T

D

A

P

P

E

D

M

I

V

K

G

E

E

L

V

I

L

A

S

I

V

I

M

H

K

K

Q

I

L

R

A

Q

N

H

E

G

G

I

M

T

T

I

M

I

V

L

V

I

V

E

T

H

H

H

E

M

M

D

G

V

F

V

A

M

R

S

E

I

V

S

G

D

D

T

R

V

V

T

L

V

F

L

M

D

D

F

G

G

G

Q

Y

K

I

I

I

A

E

E

E

G

G

R

K

P

P

binding adenosine-5'-diphosphate: F13 (= F403), V18 (≠ A408), S38 (≠ N428), G39 (= G429), S40 (= S430), G41 (= G431), K42 (= K432), S43 (= S433), T44 (= T434)

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
26% identity, 37% coverage: 394:640/661 of query aligns to 3:235/235 of 6mhzA

query
sites
6mhzA

L

L

S

T

G

A

K

K

Q

N

I

L

L

A

M

K

Q

A

F
x
Y

G

K

G

G

L

R

K

R

A

V

L

V

N

E

E

D

V

V

N

S

L

L

D

T

I

V

V

N

K

S

G

G

S

E

V

I

H

V

G

G

L

L

I

L

G

G

P

P

N
|
N

G
|
G

S

A

G
|
G

K
|
K

S
x
T

T
|
T

M

T

M

F

N

Y

V

M

L

V

T

V

G

G

I

I

Y

V

K

P

P

R

T

D

S

A

G

G

K

N

V

I

E

I

F

I

N

D

G

D

R

D

T

D

I

I

S

S

G

L

T

L

T

P

P

L

S

H

A

A

I

R

A

A

L

R

G

R

G

G

V

I

A

G

R

Y

T

L

F

P

Q
|
Q

N

E

V

A

Q

S

L

I

F

F

G

R

E

R

M

L

T

S

A

V

T

Y

E

D

N

N

V

L

L

M

V

A

G

-

L

-

H

-

H

-

T

-

F

-

R

-

S

-

N

-

M

V

L

L

D

Q

V

I

V

R

F

D

N

D

T

L

P

S

R

A

Y

E

R

Q

R

R

E

E

E

D

Q

-

S

-

A

-

R

-

E

-

R

R

A

A

A

N

S

E

I

L

L

M

Q

E

F

E

V

F

G

H

L

I

T

E

D

H

L

L

A

R

T

D

E

S

E

M

A

G

R

Q

N
x
S

L

L

P
x
S

Y
x
G

G
|
G

K

E

Q

R

R

R

L

R

L

V

E

E

I

I

G

A

R

R

A

A

L

L

G

A

L

A

N

N

P

P

E

K

L

F

L

I

L

L

L

L

D

D

E
|
E

P

P

A

F

A

A

G
|
G

L

V

T

D

A

P

P

I

D

S

I

V

K

I

E

D

L

I

I

K

A

R

I

I

I

I

H

E

K

H

I

L

R

R

Q

D

H

S

G

G

I

L

T

G

I

V

I

L

L

I

I

T

E

D

H
|
H

H

N

M

V

D

R

V

E

V

T

M

L

S

A

I

V

S

C

D

E

T

R

V

A

T

Y

V

I

L

V

D

S

F

Q

G

G

Q

H

K

L

I

I

A

A

E

H

G

G

R

T

P

P

A

T

A

E

V

I

Q

L

V

Q

D

D

P

E

K

H

V

V

I

K

E

R

A

V

Y

Y

L

L

G

G

binding adp orthovanadate: Y12 (≠ F403), N37 (= N428), G38 (= G429), G40 (= G431), K41 (= K432), T42 (≠ S433), T43 (= T434), Q84 (= Q475), S136 (≠ N541), S138 (≠ P543), G139 (≠ Y544), G140 (= G545), E162 (= E567), G166 (= G571), H194 (= H599)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
26% identity, 37% coverage: 394:640/661 of query aligns to 3:235/238 of 6s8nB

query
sites
6s8nB

L

L

S

T

G

A

K

K

Q

N

I

L

L

A

M

K

Q

A

F

Y

G

K

G

G

L

R

K

R

A

V

L

V

N

E

E

D

V

V

N

S

L

L

D

T

I

V

V

N

K

S

G

G

S

E

V

I

H

V

G

G

L

L

I

L

G

G

P

P

N

N

G

G

S

A

G

G

K

K

S

T

T

T

M

T

M

F

N

Y

V

M

L

V

T

V

G

G

I

I

Y

V

K

P

P

R

T

D

S

A

G

G

K

N

V

I

E

I

F

I

N

D

G

D

R

D

T

D

I

I

S

S

G

L

T

L

T

P

P

L

S

H

A

A

I

R

A

A

L

R

G

R

G

G

V

I

A

G

R

Y

T

L

F

P

Q

Q

N

E

V

A

Q

S

L

I

F

F

G
x
R

E

R

M

L

T

S

A

V

T

Y

E

D

N

N

V

L

L

M

V

A

G

-

L

-

H

-

H

-

T

-

F

-

R

-

S

-

N

-

M

V

L

L

D

Q

V

I

V

R

F

D

N

D

T

L

P

S

R

A

Y

E

R

Q

R

R

E

E

E

D

Q

-

S

-

A

-

R

-

E

-

R

R

A

A

A

N

S

E

I

L

L

M

Q

E

F

E

V

F

G

H

L

I

T

E

D

H

L

L

A

R

T

D

E

S

E
x
M

A
x
G

R
x
Q

N

S

L

L

P

S

Y

G

G

G

K

E

Q

R

R

R

L

R

L

V

E

E

I

I

G

A

R

R

A

A

L

L

G

A

L

A

N

N

P

P

E

K

L

F

L

I

L

L

L

L

D

D

E

E

P

P

A

F

A

A

G

G

L

V

T

D

A

P

P

I

D

S

I

V

K

I

E

D

L

I

I

K

A

R

I

I

I

I

H

E

K

H

I

L

R

R

Q

D

H

S

G

G

I

L

T

G

I

V

I

L

L

I

I

T

E

D

H

H

H

N

M

V

D

R

V

E

V

T

M

L

S

A

I

V

S

C

D

E

T

R

V

A

T

Y

V

I

L

V

D

S

F

Q

G

G

Q

H

K

L

I

I

A

A

E

H

G

G

R

T

P

P

A

T

A

E

V

I

Q

L

V

Q

D

D

P

E

K

H

V

V

I

K

E

R

A

V

Y

Y

L

L

G

G

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ G481), M133 (≠ E538), G134 (≠ A539), Q135 (≠ R540)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
26% identity, 37% coverage: 394:640/661 of query aligns to 3:235/238 of 6s8gA

query
sites
6s8gA

L

L

S

T

G

A

K

K

Q

N

I

L

L

A

M

K

Q

A

F
x
Y

G

K

G

G

L
x
R

K

R

A

V

L

V

N

E

E

D

V

V

N

S

L

L

D

T

I

V

V

N

K

S

G

G

S

E

V

I

H

V

G

G

L

L

I

L

G

G

P

P

N
|
N

G

G

S

A

G
|
G

K
|
K

S
x
T

T
|
T

M

T

M

F

N

Y

V

M

L

V

T

V

G

G

I

I

Y

V

K

P

P

R

T

D

S

A

G

G

K

N

V

I

E

I

F

I

N

D

G

D

R

D

T

D

I

I

S

S

G

L

T

L

T

P

P

L

S

H

A

A

I

R

A

A

L

R

G

R

G

G

V

I

A

G

R

Y

T

L

F

P

Q
|
Q

N

E

V

A

Q

S

L

I

F

F

G

R

E

R

M

L

T

S

A

V

T

Y

E

D

N

N

V

L

L

M

V

A

G

-

L

-

H

-

H

-

T

-

F

-

R

-

S

-

N

-

M

V

L

L

D

Q

V

I

V

R

F

D

N

D

T

L

P

S

R

A

Y

E

R

Q

R

R

E

E

E

D

Q

-

S

-

A

-

R

-

E

-

R

R

A

A

A

N

S

E

I

L

L

M

Q

E

F

E

V

F

G

H

L

I

T

E

D

H

L

L

A

R

T

D

E

S

E

M

A

G

R

Q

N
x
S

L

L

P
x
S

Y

G

G

G

K
x
E

Q

R

R

R

L

R

L

V

E

E

I

I

G

A

R

R

A

A

L

L

G

A

L

A

N

N

P

P

E

K

L

F

L

I

L

L

L

L

D

D

E

E

P

P

A

F

A

A

G

G

L

V

T

D

A

P

P

I

D

S

I

V

K

I

E

D

L

I

I

K

A

R

I

I

I

I

H

E

K

H

I

L

R

R

Q

D

H

S

G

G

I

L

T

G

I

V

I

L

L

I

I

T

E

D

H

H

H

N

M

V

D

R

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