SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_061533781.1 NCBI__GCF_001584165.1:WP_061533781.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1u08A Crystal structure and reactivity of ybdl from escherichia coli identify a methionine aminotransferase function. (see paper)
51% identity, 96% coverage: 18:396/396 of query aligns to 5:382/382 of 1u08A

query
sites
1u08A

K

Q

S

S

K

K

L

L

P

P

R

Q

V

L

G

G

T

T

I

I

F

F

T

T

V

Q

M

M

S

S

T

A

L

L

A

A

S

Q

E

Q

K

H

S

Q

A

A

V

I

N

N

L

L

G

S

Q

Q

G

G

F

F

P

P

D

D

F

F

D

D

C

G

D

P

P

R

A

Y

L

L

V

Q

N

E

A

R

V

L

S

A

D

H

A

H

M

V

K

A

N

Q

G

G

F

A

N

N

Q

Q

Y

Y

P

A

P

P

M

M

T

T

G

G

V

V

P

Q

A

A

L

L

R

R

Q

E

A

A

I

I

A

A

A

Q

K

K

I

T

E

E

K

R

L

L

Y

Y

G

G

H

Y

S

Q

Y

P

D

D

P

A

A

D

T

S

D

D

I

I

T

T

V

V

T

T

A

A

G
|
G

A
|
A

T
|
T

Q

E

G

A

L

L

L

Y

T

A

A

A

I

I

M

T

C

A

A

L

V

V

H

R

P

N

G

G

D

D

E

E

V

V

I

I

V

C

I

F

E

D

P

P

A

S

Y
|
Y

D

D

S

S

Y

Y

V

A

P

P

A

A

I

I

E

A

L

L

A

S

G

G

K

-

P

-

V

I

L

V

V

K

Q

R

M

M

T

A

L

L

G

Q

A

P

D

P

G

H

Y

F

A

R

I

V

P

D

W

W

D

Q

Q

E

I

F

A

A

A

L

V

L

S

S

P

E

R

R

T

T

R

R

M

L

I

V

M

I

I

L

N
|
N

S

T

P

P

H

H

N

N

P

P

T

S

G

A

S

T

V

V

L

W

N

Q

L

Q

A

A

D

D

I

F

E

A

A

A

L

L

K

W

D

Q

I

A

V

I

R

A

G

G

T

H

D

E

I

I

L

F

I

V

L

I

S

S

D
|
D

E

E

V
|
V

Y
|
Y

E

E

H

H

M

I

V

N

Y

F

D

S

G

Q

L

Q

R

G

H

H

E

A

S

S

I

V

C

L

R

A

H

H

P

P

E

Q

L

L

A

R

E

E

R

R

A

A

F

V

I

A

V

V

S

S

S
|
S

F

F

G

G

K
|
K

T

T

Y

Y

H

H

V

M

T

T

G

G

W

W

K
|
K

I

V

G

G

Y

Y

V

C

A

V

A

A

P

P

P

A

A

P

L

I

S

S

A

A

E

E

F

I

R

R

K

K

V

V

H

H

Q

Q

Y

Y

N

L

I

T

F

F

T

S

V

V

N

N

T

T

P

P

V

A

Q

Q

H

L

G

A

I

L

A

A

T

D

Y

M

M

L

Q

R

-

A

N

E

P

P

A

E

P

H

Y

Y

L

L

E

A

L

L

A

P

A

D

F

F

Y

Y

Q

R

R

Q

K

K

R

R

D

D

L

I

F

L

R

V

D

N

G

A

L

L

K

N

H

E

T

S

R

R

F

L

K

E

L

I

L

L

P

P

A

C

D

E

G

G

T

T

Y

Y

F

F

L

L

C

L

V

V

D

D

Y

Y

S

S

A

A

I

V

S

S

A

T

M

L

S

D

E

D

A

V

D

E

F

F

A

C

V

Q

W

W

L

L

T

T

S

Q

E

E

I

H

G

G

V

V

A

A

I

I

P

P

V

L

S

S

A

V

F

F

Y

C

D

A

S

D

P

P

R

F

E

P

S

H

G

K

I

L

V

I

R

R

F

L

C

C

F

F

A

A

K

K

Q

K

D

E

Q

S

T

T

L

L

Q

L

Q

A

A

A

L

A

Q

E

R

R

L

L

A

R

K

Q

I

L

binding pyridoxal-5'-phosphate: G95 (= G108), A96 (= A109), T97 (= T110), Y121 (= Y134), N166 (= N181), D198 (= D213), V200 (= V215), Y201 (= Y216), S229 (= S244), K232 (= K247), K240 (= K255)

2o0rA The three-dimensional structure of n-succinyldiaminopimelate aminotransferase from mycobacterium tuberculosis (see paper)
41% identity, 95% coverage: 19:396/396 of query aligns to 4:383/385 of 2o0rA

query
sites
2o0rA

S

S

K

R

L

L

P

R

R

P

V

Y

G

A

T

T

I

V

F
|
F

T

A

V

E

M

M

S

S

T

A

L

L

A

A

S

T

E

R

K

I

S

G

A

A

V

V

N

N

L

L

G

G

Q

Q

G

G

F

F

P

P

D

D

F

E

D

D

C

G

D

P

P

P

A

K

L

M

V

L

N

Q

A

A

V

A

S

Q

D

D

A

A

M

I

K

A

N

G

G

G

F

V

N

N

Q

Q

Y

Y

P

P

M

G

T

P

G

G

V

S

P

A

A

P

L

L

R

R

Q

R

A

A

I

I

A

A

K

Q

I

R

E

R

K

R

L

H

Y

F

G

G

H

V

S

D

Y

Y

D

D

P

P

A

E

T

T

D

E

I

V

T

L

V

V

T

T

A

V

G

G

A

A

T

T

Q

E

G

A

L

I

L

A

T

A

A

A

I

V

M

L

C

G

A

L

V

V

H

E

P

P

G

G

D

S

E

E

V

V

I

L

V

L

I

I

E

E

P

P

A

F

Y
|
Y

D
|
D

S

S

Y

Y

V

S

P

P

A

V

I

V

E

A

L

M

A

A

G

G

G

A

K

H

P

R

V

V

L

T

V

V

Q

P

M

L

T

V

L

P

G

D

A

G

D

R

G

G

Y

F

A

A

I

L

P

D

W

A

D

D

Q

A

I

L

A

R

A

A

V

V

S

T

P

P

R

R

T

T

R

R

M

A

I

L

M

I

I

I

N

N

S

S

P

P

H

H

N

N

P

P

T

T

G

G

S

A

V

V

L

L

N

S

L

A

A

T

D

E

I

L

E

A

A

A

L

I

K

A

D

E

I

I

V

A

R

V

G

A

T

A

D

N

I

L

L

V

I

V

L

I

S

T

D
|
D

E

E

V
|
V

Y

Y

E

E

H

H

M

L

V

V

Y

F

D

D

G

H

L

A

R

R

H

H

E

L

S

P

I

L

C

A

R

G

H

F

P

D

E

G

L

M

A

A

E

E

R

R

A

T

F

I

I

T

V

I

S

S

F

A

G

A

K
|
K

T

M

Y

F

H

N

V

C

T

T

G

G

W

W

K

K

I

I

G

G

Y

W

V

A

A

C

A

G

P

P

P

A

A

E

L

L

S

I

A

A

E

G

F

V

R

R

K

A

V

A

H

K

Q

Q

Y

Y

N

L

I

S

F

Y

T

V

V

G

N

G

T

A

P

P

V

F

Q

Q

H

P

G

A

I

V

A

A

T

L

Y

A

M

L

Q

D

N

T

P

E

A

D

P

A

Y

W

L

V

-

A

E

A

L

L

A

R

A

N

F

S

Y

L

Q

R

R

A

K

R

R

R

D

D

L

R

F

L

R

A

D

A

G

G

L

L

K

T

H

E

T

I

R

G

F

F

K

A

L

V

L

H

P

D

A

S

D

Y

G

G

T

T

Y

Y

F

F

L

L

C

C

V

A

D

D

Y

P

S

R

A

P

I

L

S

G

A

Y

M

D

S

D

E

S

A

T

D

E

F

F

A

C

V

A

W

A

L

L

T

P

S

E

E

K

I

V

G

G

V

V

A

A

I

I

P

P

V

M

S

S

A

A

F

F

Y

C

D

D

-

P

S

A

P

D

R

V

E

W

S

N

G

H

I

L

V

V

R

R

F

F

C

T

F

F

A

C

K

K

Q

R

D

D

Q

D

T

T

L

L

Q

D

Q

E

A

A

L

I

Q

R

R

R

L

L

A

S

K

V

I

L

active site: Y119 (= Y134), D198 (= D213), V200 (= V215), K232 (= K247)
binding glycerol: F14 (= F29), D120 (= D135)

Q17CS8 Kynurenine aminotransferase; AeKAT; EC 2.6.1.-; EC 2.6.1.63; EC 2.6.1.7 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see 2 papers)
34% identity, 91% coverage: 35:395/396 of query aligns to 81:472/477 of Q17CS8

query
sites
Q17CS8

L

L

A

A

S

A

E

Q

K

Y

S

K

A

P

V

L

N

N

L

L

G

G

Q

Q

G

G

F

F

P

P

D

D

F

Y

D

H

C

A

D

-

P

P

A

K

L

Y

V

A

N

L

A

D

V

A

S

L

D

A

A

A

M

A

K

A

N

N

G

S

-

P

-

D

-

P

-

L

F

A

N

N

Q

Q

Y
|
Y

P

T

P

R

M

G

T

F

G

G

V

H

P

P

A

R

L

L

R

V

Q

Q

A

A

I

L

A

S

A

K

K

L

I

Y

E

S

K

Q

L

L

Y

V

G

D

H

R

S

T

Y

I

D

N

P

P

A

M

T

T

D

E

I

V

T

L

V

V

T

T

A

V

G

G

A
|
A

T
x
Y

Q

E

G

A

L

L

L

Y

T

A

A

T

I

I

M

Q

C

G

A

H

V

V

H

D

P

E

G

G

D

D

E

E

V

V

I

I

V

I

I

I

E

E

P

P

A

F

Y

F

D

D

S

C

Y

Y

V

E

P

P

A

M

I

V

E

K

L

A

A

A

G

G

K

I

P

P

V

R

L

F

V

I

Q

P

M

L

-

K

-

P

-

N

-

K

-

T

-

G

T

T

L

I

G

S

A

S

D

A

G

D

Y

W

A

V

I

L

P

D

W

N

D

N

Q

E

I

L

A

E

A

A

A

L

V

F

S

N

P

E

R

K

T

T

R

K

M

M

I

I

M

I

I

I

N

N

S

T

P

P

H

H

N
|
N

P

P

T

L

G

G

S

K

V

V

L

M

N

D

L

R

A

A

D

E

I

L

E

E

A

V

L

V

K

A

D

N

I

L

V

C

R

K

G

K

T

W

D

N

I

V

L

L

I

C

L

V

S

S

D

D

E

E

V

V

Y
|
Y

E

E

H

H

M

M

V

V

Y

F

D

E

G

P

L

F

R

E

H

H

E

I

S

R

I

I

C

C

R

T

H

L

P

P

E

G

L

M

A

W

E

E

R

R

A

T

F

I

I

T

V

I

S

G

S
|
S

F

A

G

G

K
|
K

T

T

Y

F

H

S

V

L

T

T

G

G

W

W

K
|
K

I

I

G

G

Y

W

V

A

A

Y

A

G

P

P

P

E

A

A

L

L

S

L

A

K

E

N

F

L

R

Q

K

M

V

V

H

H

Q

Q

Y

N

N

C

I

V

F

Y

T

T

V

C

N

A

T

T

P

P

V

I

Q

Q

H

E

G

A

I

I

A

A

T

V

-

G

-

F

-

E

-

T

-

E

-

L

-

K

Y

R

M

L

Q

K

N

S

P

P

A

E

P

C

Y

Y

L

F

E

N

-

S

L

I

A

S

A

G

F

E

Y

L

Q

M

R

A

K

K

R

R

D

D

L

Y

F

M

R

A

D

S

G

F

L

L

K

A

H

E

T

V

R

G

F

M

K

N

L

P

L

T

P

V

A

P

D

Q

G

G

T

G

Y

Y

F

F

L

M

C

V

V

A

D

D

Y

W

S

S

A

S

I

L

-

D

-

S

-

K

-

V

-

D

-

L

-

T

-

Q

-

E

-

T

S

D

A

A

M

R

S

K

E

D

A

Y

D

R

F

F

A

T

V

K

W

W

L

M

T

T

S

K

E

S

I

V

G

G

V

L

A

Q

A

G

I

I

P

P

V

P

S

S

A

A

F

F

Y

Y

D

S

S

E

P

P

R

N

E

K

-

H

-

L

-

G

S

E

G

D

I

F

V

V

R
|
R

F

Y

C

C

F

F

A

F

K

K

Q

K

D

D

Q

E

T

N

L

L

Q

Q

Q

K

A

A

L

A

Q

E

R

I

L

L

A

R

K

K

Y121 (= Y72) binding pyridoxal 5'-phosphate
AY 158:159 (≠ AT 109:110) binding in other chain
N241 (= N185) binding in other chain
Y272 (= Y216) binding in other chain
S300 (= S244) binding in other chain
K303 (= K247) modified: N6-(pyridoxal phosphate)lysine
K311 (= K255) binding in other chain
R453 (= R376) binding substrate

Sites not aligning to the query:

44:45 binding substrate

1w7nA Crystal structure of human kynurenine aminotransferase i in pmp form (see paper)
35% identity, 91% coverage: 35:395/396 of query aligns to 21:411/415 of 1w7nA

query
sites
1w7nA

L

L

A

A

S

S

E

E

K

H

S

D

A

V

V

V

N

N

L

L

G

G

Q

Q

G

G

F

F

P

P

D

D

F

F

D

P

C

P

D

P

P

D

A

F

L

A

V

V

N

E

A

A

V

F

S

Q

D

H

A

A

M

V

K

S

N

G

G

D

F

F

-

M

-

L

N

N

Q

Q

Y

Y

P

T

P

K

M

T

T

F

G

G

V

Y

P

P

A

P

L

L

R

T

Q

K

A

I

I

L

A

A

A

S

K

F

I

F

E

G

K

E

L

L

Y

L

G

G

H

Q

S

E

Y

I

D

D

P

P

A

L

T

R

D

N

I

V

T

L

V

V

T

T

A

V

G
|
G

A
x
G

T
x
Y

Q

G

G

A

L

L

L

F

T

T

A

A

I

F

M

Q

C

A

A

L

V

V

H

D

P

E

G

G

D

D

E

E

V

V

I

I

V

I

I

I

E

E

P

P

A

F

Y
x
F

D

D

S

C

Y

Y

V

E

P

P

A

M

I

T

E

M

L

M

A

A

G

G

K

R

P

P

V

V

L

F

V

V

Q

S

M

L

T

K

L

P

G

G

A

P

D

G

-

E

-

L

G

G

Y

S

A

S

I

S

P

N

W

W

D

Q

-

L

-

D

-

P

-

M

Q

E

I

L

A

A

A

G

A

K

V

F

S

T

P

S

R

R

T

T

R

K

M

A

I

L

M

V

I

L

N
|
N

S

T

P

P

H

N

N

N

P

P

T

L

G

G

S

K

V

V

L

F

N

S

L

R

A

E

D

E

I

L

E

E

A

L

L

V

K

A

D

S

I

L

V

C

R

Q

G

Q

T

H

D

D

I

V

L

V

I

C

L

I

S

T

D
|
D

E

E

V
|
V

Y
|
Y

E

Q

H

W

M

M

V

V

Y

Y

D

D

G

G

L

H

R

Q

H

H

E

I

S

S

I

I

C

A

R

S

H

L

P

P

E

G

L

M

A

W

E

E

R

R

A

T

F

L

I

T

V

I

S

G

S
|
S

F

A

G

G

K
|
K

T

T

Y

F

H

S

V

A

T

T

G

G

W

W

K
|
K

I

V

G

G

Y

W

V

V

A

L

A

G

P

P

P

D

A

H

L

I

S

M

A

K

E

H

F

L

R

R

K

T

V

V

H

H

Q

Q

Y

N

N

S

I

V

F

F

T

H

V

C

N

P

T

T

P

Q

V

S

Q

Q

H

A

G

A

I

V

A

A

T

E

-

S

-

F

-

E

-

R

-

E

-

Q

-

L

Y

F

M

R

Q

Q

N

P

P

S

A

S

P

Y

Y

F

L

V

E

Q

L

F

A

P

A

Q

F

A

Y

M

Q

Q

R

R

K

C

R

R

D

D

L

H

F

M

R

I

D

R

G

S

L

L

K

Q

H

S

T

V

R

G

F

L

K

K

L

P

L

L

P

I

A

P

D

Q

G

G

T

S

Y

Y

F

F

L

L

C

I

V

T

D

D

Y

I

S

S

-

D

-

F

-

K

-

R

-

K

-

M

-

P

-

D

-

L

-

P

-

G

A

A

I

V

S

D

A

E

M

P

S

Y

E

D

A

R

D

R

F

F

A

V

V

K

W

W

L

M

T

I

S

K

E

N

I

K

G

G

V

L

A

V

A

A

I

I

P

P

V

V

S

S

A

I

F

F

Y

Y

D

S

S

V

P

P

R

H

E

Q

S

K

G

H

I

F

-

D

-

H

-

Y

V

I

R

R

F

F

C

C

F

F

A

V

K

K

Q

D

D

E

Q

A

T

T

L

L

Q

Q

Q

A

A

M

L

D

Q

E

R

K

L

L

A

R

K

K

active site: F122 (≠ Y134), D207 (= D213), V209 (= V215), K241 (= K247)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G96 (= G108), G97 (≠ A109), Y98 (≠ T110), F122 (≠ Y134), N175 (= N181), D207 (= D213), V209 (= V215), Y210 (= Y216), S238 (= S244), K241 (= K247), K249 (= K255)

1w7mA Crystal structure of human kynurenine aminotransferase i in complex with l-phe (see paper)
35% identity, 91% coverage: 35:395/396 of query aligns to 21:411/415 of 1w7mA

query
sites
1w7mA

L

L

A

A

S

S

E

E

K

H

S

D

A

V

V

V

N

N

L

L

G

G

Q

Q

G
|
G

F

F

P

P

D

D

F

F

D

P

C

P

D

P

P

D

A

F

L

A

V

V

N

E

A

A

V

F

S

Q

D

H

A

A

M

V

K

S

N

G

G

D

F

F

-

M

-

L

N

N

Q

Q

Y

Y

P

T

P

K

M

T

T

F

G

G

V

Y

P

P

A

P

L

L

R

T

Q

K

A

I

I

L

A

A

A

S

K

F

I

F

E

G

K

E

L

L

Y

L

G

G

H

Q

S

E

Y

I

D

D

P

P

A

L

T

R

D

N

I

V

T

L

V

V

T

T

A

V

G
|
G

A
x
G

T
x
Y

Q

G

G

A

L

L

L

F

T

T

A

A

I

F

M

Q

C

A

A

L

V

V

H

D

P

E

G

G

D

D

E

E

V

V

I

I

V

I

I

I

E

E

P

P

A

F

Y
x
F

D

D

S

C

Y

Y

V

E

P

P

A

M

I

T

E

M

L

M

A

A

G

G

K

R

P

P

V

V

L

F

V

V

Q

S

M

L

T

K

L

P

G

G

A

P

D

G

-

E

-

L

G

G

Y

S

A

S

I

S

P

N

W

W

D

Q

-

L

-

D

-

P

-

M

Q

E

I

L

A

A

A

G

A

K

V

F

S

T

P

S

R

R

T

T

R

K

M

A

I

L

M

V

I

L

N
|
N

S

T

P

P

H

N

N
|
N

P

P

T

L

G

G

S

K

V

V

L

F

N

S

L

R

A

E

D

E

I

L

E

E

A

L

L

V

K

A

D

S

I

L

V

C

R

Q

G

Q

T

H

D

D

I

V

L

V

I

C

L

I

S

T

D
|
D

E

E

V
|
V

Y
|
Y

E

Q

H

W

M

M

V

V

Y

Y

D

D

G

G

L

H

R

Q

H

H

E

I

S

S

I

I

C

A

R

S

H

L

P

P

E

G

L

M

A

W

E

E

R

R

A

T

F

L

I

T

V

I

S

G

S
|
S

F

A

G

G

K
|
K

T

T

Y

F

H

S

V

A

T

T

G

G

W

W

K
|
K

I

V

G

G

Y

W

V

V

A

L

A

G

P

P

P

D

A

H

L

I

S

M

A

K

E

H

F

L

R

R

K

T

V

V

H

H

Q

Q

Y

N

N

S

I

V

F

F

T

H

V

C

N

P

T

T

P

Q

V

S

Q

Q

H

A

G

A

I

V

A

A

T

E

-

S

-

F

-

E

-

R

-

E

-

Q

-

L

Y

F

M

R

Q

Q

N

P

P

S

A

S

P

Y

Y

F

L

V

E

Q

L

F

A

P

A

Q

F

A

Y

M

Q

Q

R

R

K

C

R

R

D

D

L

H

F

M

R

I

D

R

G

S

L

L

K

Q

H

S

T

V

R

G

F

L

K

K

L

P

L

L

P

I

A

P

D

Q

G

G

T

S

Y

Y

F

F

L

L

C

I

V

T

D

D

Y

I

S

S

-

D

-

F

-

K

-

R

-

K

-

M

-

P

-

D

-

L

-

P

-

G

A

A

I

V

S

D

A

E

M

P

S

Y

E

D

A

R

D

R

F

F

A

V

V

K

W

W

L

M

T

I

S

K

E

N

I

K

G

G

V

L

A

V

A

A

I

I

P

P

V

V

S

S

A

I

F

F

Y

Y

D

S

S

V

P

P

R

H

E

Q

S

K

G

H

I

F

-

D

-

H

-

Y

V

I

R
|
R

F

F

C

C

F

F

A

V

K

K

Q

D

D

E

Q

A

T

T

L

L

Q

Q

Q

A

A

M

L

D

Q

E

R

K

L

L

A

R

K

K

active site: F122 (≠ Y134), D207 (= D213), V209 (= V215), K241 (= K247)
binding phenylalanine: G33 (= G47), Y98 (≠ T110), F122 (≠ Y134), N179 (= N185), R392 (= R376)
binding pyridoxal-5'-phosphate: G96 (= G108), G97 (≠ A109), Y98 (≠ T110), F122 (≠ Y134), N175 (= N181), N179 (= N185), D207 (= D213), V209 (= V215), Y210 (= Y216), S238 (= S244), K241 (= K247), K249 (= K255)

Sites not aligning to the query:

binding phenylalanine: 15

1w7lA Crystal structure of human kynurenine aminotransferase i (see paper)
35% identity, 91% coverage: 35:395/396 of query aligns to 21:411/415 of 1w7lA

query
sites
1w7lA

L

L

A

A

S

S

E

E

K

H

S

D

A

V

V

V

N

N

L

L

G

G

Q

Q

G

G

F

F

P

P

D

D

F

F

D

P

C

P

D

P

P

D

A

F

L

A

V

V

N

E

A

A

V

F

S

Q

D

H

A

A

M

V

K

S

N

G

G

D

F

F

-

M

-

L

N

N

Q

Q

Y

Y

P

T

P

K

M

T

T

F

G

G

V

Y

P

P

A

P

L

L

R

T

Q

K

A

I

I

L

A

A

A

S

K

F

I

F

E

G

K

E

L

L

Y

L

G

G

H

Q

S

E

Y

I

D

D

P

P

A

L

T

R

D

N

I

V

T

L

V

V

T

T

A

V

G
|
G

A
x
G

T
x
Y

Q

G

G

A

L

L

L

F

T

T

A

A

I

F

M

Q

C

A

A

L

V

V

H

D

P

E

G

G

D

D

E

E

V

V

I

I

V

I

I

I

E

E

P

P

A

F

Y
x
F

D

D

S

C

Y

Y

V

E

P

P

A

M

I

T

E

M

L

M

A

A

G

G

K

R

P

P

V

V

L

F

V

V

Q

S

M

L

T

K

L

P

G

G

A

P

D

G

-

E

-

L

G

G

Y

S

A

S

I

S

P

N

W

W

D

Q

-

L

-

D

-

P

-

M

Q

E

I

L

A

A

A

G

A

K

V

F

S

T

P

S

R

R

T

T

R

K

M

A

I

L

M

V

I

L

N
|
N

S

T

P

P

H

N

N
|
N

P

P

T

L

G

G

S

K

V

V

L

F

N

S

L

R

A

E

D

E

I

L

E

E

A

L

L

V

K

A

D

S

I

L

V

C

R

Q

G

Q

T

H

D

D

I

V

L

V

I

C

L

I

S

T

D
|
D

E

E

V
|
V

Y
|
Y

E

Q

H

W

M

M

V

V

Y

Y

D

D

G

G

L

H

R

Q

H

H

E

I

S

S

I

I

C

A

R

S

H

L

P

P

E

G

L

M

A

W

E

E

R

R

A

T

F

L

I

T

V

I

S

G

S
|
S

F

A

G

G

K
|
K

T

T

Y

F

H

S

V

A

T

T

G

G

W

W

K
|
K

I

V

G

G

Y

W

V

V

A

L

A

G

P

P

P

D

A

H

L

I

S

M

A

K

E

H

F

L

R

R

K

T

V

V

H

H

Q

Q

Y

N

N

S

I

V

F

F

T

H

V

C

N

P

T

T

P

Q

V

S

Q

Q

H

A

G

A

I

V

A

A

T

E

-

S

-

F

-

E

-

R

-

E

-

Q

-

L

Y

F

M

R

Q

Q

N

P

P

S

A

S

P

Y

Y

F

L

V

E

Q

L

F

A

P

A

Q

F

A

Y

M

Q

Q

R

R

K

C

R

R

D

D

L

H

F

M

R

I

D

R

G

S

L

L

K

Q

H

S

T

V

R

G

F

L

K

K

L

P

L

L

P

I

A

P

D

Q

G

G

T

S

Y

Y

F

F

L

L

C

I

V

T

D

D

Y

I

S

S

-

D

-

F

-

K

-

R

-

K

-

M

-

P

-

D

-

L

-

P

-

G

A

A

I

V

S

D

A

E

M

P

S

Y

E

D

A

R

D

R

F

F

A

V

V

K

W

W

L

M

T

I

S

K

E

N

I

K

G

G

V

L

A

V

A

A

I

I

P

P

V

V

S

S

A

I

F

F

Y

Y

D

S

S

V

P

P

R

H

E

Q

S

K

G

H

I

F

-

D

-

H

-

Y

V

I

R

R

F

F

C

C

F

F

A

V

K

K

Q

D

D

E

Q

A

T

T

L

L

Q

Q

Q

A

A

M

L

D

Q

E

R

K

L

L

A

R

K

K

active site: F122 (≠ Y134), D207 (= D213), V209 (= V215), K241 (= K247)
binding pyridoxal-5'-phosphate: G96 (= G108), G97 (≠ A109), Y98 (≠ T110), F122 (≠ Y134), N175 (= N181), N179 (= N185), D207 (= D213), V209 (= V215), Y210 (= Y216), S238 (= S244), K241 (= K247), K249 (= K255)

4wljA High resolution crystal structure of human kynurenine aminotransferase-i in complex with aminooxyacetate (see paper)
35% identity, 91% coverage: 35:395/396 of query aligns to 21:408/413 of 4wljA

query
sites
4wljA

L

L

A

A

S

S

E

E

K

H

S

D

A

V

V

V

N

N

L

L

G

G

Q

Q

G

G

F

F

P

P

D

D

F

F

D

P

C

P

D

P

P

D

A

F

L

A

V

V

N

E

A

A

V

F

S

Q

D

H

A

A

M

V

K

S

N

G

G

D

F

F

-

M

-

L

N

N

Q

Q

Y

Y

P

T

P

K

M

T

T

F

G

G

V

Y

P

P

A

P

L

L

R

T

Q

K

A

I

I

L

A

A

A

S

K

F

I

F

E

G

K

E

L

L

Y

L

G

G

H

Q

S

E

Y

I

D

D

P

P

A

L

T

R

D

N

I

V

T

L

V

V

T

T

A

V

G
|
G

A
x
G

T
x
Y

Q

G

G

A

L

L

L

F

T

T

A

A

I

F

M

Q

C

A

A

L

V

V

H

D

P

E

G

G

D

D

E

E

V

V

I

I

V

I

I

I

E

E

P

P

A

F

Y
x
F

D

D

S

C

Y

Y

V

E

P

P

A

M

I

T

E

M

L

M

A

A

G

G

K

R

P

P

V

V

L

F

V

V

Q

S

M

L

T

K

L

P

G

G

A

L

D

-

G

G

Y

S

A

S

I

S

P

N

W

W

D

Q

-

L

-

D

-

P

-

M

Q

E

I

L

A

A

A

G

A

K

V

F

S

T

P

S

R

R

T

T

R

K

M

A

I

L

M

V

I

L

N
|
N

S

T

P

P

H

N

N
|
N

P

P

T

L

G

G

S

K

V

V

L

F

N

S

L

R

A

E

D

E

I

L

E

E

A

L

L

V

K

A

D

S

I

L

V

C

R

Q

G

Q

T

H

D

D

I

V

L

V

I

C

L

I

S

T

D
|
D

E

E

V
|
V

Y
|
Y

E

Q

H

W

M

M

V

V

Y

Y

D

D

G

G

L

H

R

Q

H

H

E

I

S

S

I

I

C

A

R

S

H

L

P

P

E

G

L

M

A

W

E

E

R

R

A

T

F

L

I

T

V

I

S

G

S
|
S

F

A

G

G

K
|
K

T

T

Y

F

H

S

V

A

T

T

G

G

W

W

K
|
K

I

V

G

G

Y

W

V

V

A

L

A

G

P

P

P

D

A

H

L

I

S

M

A

K

E

H

F

L

R

R

K

T

V

V

H

H

Q

Q

Y

N

N

S

I

V

F

F

T

H

V

C

N

P

T

T

P

Q

V

S

Q

Q

H

A

G

A

I

V

A

A

T

E

-

S

-

F

-

E

-

R

-

E

-

Q

-

L

Y

F

M

R

Q

Q

N

P

P

S

A

S

P

Y

Y

F

L

V

E

Q

L

F

A

P

A

Q

F

A

Y

M

Q

Q

R

R

K

C

R

R

D

D

L

H

F

M

R

I

D

R

G

S

L

L

K

Q

H

S

T

V

R

G

F

L

K

K

L

P

L

I

P

I

A

P

D

Q

G

G

T

S

Y

Y

F

F

L

L

C

I

V

T

D

D

Y

I

S

S

-

D

-

F

-

K

-

R

-

K

-

M

-

P

-

D

-

L

-

P

-

G

A

A

I

V

S

D

A

E

M

P

S

Y

E

D

A

R

D

R

F

F

A

V

V

K

W

W

L

M

T

I

S

K

E

N

I

K

G

G

V

L

A

V

A

A

I

I

P

P

V

V

S

S

A

I

F

F

Y

Y

D

S

S

V

P

P

R

H

E

Q

S

K

G

H

I

F

-

D

-

H

-

Y

V

I

R
|
R

F

F

C

C

F

F

A

V

K

K

Q

D

D

E

Q

A

T

T

L

L

Q

Q

Q

A

A

M

L

D

Q

E

R

K

L

L

A

R

K

K

active site: F122 (≠ Y134), D204 (= D213), V206 (= V215), K238 (= K247)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: G96 (= G108), G97 (≠ A109), Y98 (≠ T110), F122 (≠ Y134), N172 (= N181), N176 (= N185), D204 (= D213), V206 (= V215), Y207 (= Y216), S235 (= S244), K238 (= K247), K246 (= K255), R389 (= R376)

Sites not aligning to the query:

binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: 15

2r5eA Aedes kynurenine aminotransferase in complex with glutamine (see paper)
34% identity, 91% coverage: 35:395/396 of query aligns to 23:414/419 of 2r5eA

query
sites
2r5eA

L

L

A

A

S

A

E

Q

K

Y

S

K

A

P

V

L

N

N

L

L

G

G

Q
|
Q

G

G

F

F

P

P

D

D

F

Y

D

H

C

A

D

P

P

K

A

Y

L

A

V

L

N

N

A

A

V

L

S

A

D

A

A

A

M

A

K

N

N

S

G

P

-

D

-

P

-

L

F

A

N

N

Q

Q

Y
|
Y

P

T

P

R

M

G

T

F

G

G

V

H

P

P

A

R

L

L

R

V

Q

Q

A

A

I

L

A

S

A

K

K

L

I

Y

E

S

K

Q

L

L

Y

V

G

D

H

R

S

T

Y

I

D

N

P

P

A

M

T

T

D

E

I

V

T

L

V

V

T

T

A

V

G
|
G

A
|
A

T
x
Y

Q

E

G

A

L

L

L

Y

T

A

A

T

I

I

M

Q

C

G

A

H

V

V

H

D

P

E

G

G

D

D

E

E

V

V

I

I

V

I

I

I

E

E

P

P

A

F

Y
x
F

D

D

S

C

Y

Y

V

E

P

P

A

M

I

V

E

K

L

A

A

A

G

G

K

I

P

P

V

R

L

F

V

I

Q

P

M

L

-

K

-

P

-

N

-

K

-

T

-

G

T

T

L

I

G

S

A

S

D

A

G

D

Y

W

A

V

I

L

P

D

W

N

D

N

Q

E

I

L

A

E

A

A

A

L

V

F

S

N

P

E

R

K

T

T

R

K

M

M

I

I

M

I

I

I

N
|
N

S

T

P

P

H

H

N
|
N

P

P

T

L

G

G

S

K

V

V

L

M

N

D

L

R

A

A

D

E

I

L

E

E

A

V

L

V

K

A

D

N

I

L

V

C

R

K

G

K

T

W

D

N

I

V

L

L

I

C

L

V

S

S

D
|
D

E

E

V
|
V

Y
|
Y

E

E

H

H

M

M

V

V

Y

F

D

E

G

P

L

F

R

E

H

H

E

I

S

R

I

I

C

C

R

T

H

L

P

P

E

G

L

M

A

W

E

E

R

R

A

T

F

I

I

T

V

I

S

G

S
|
S

F

A

G

G

K
|
K

T

T

Y

F

H

S

V

L

T

T

G

G

W

W

K
|
K

I

I

G

G

Y

W

V

A

A

Y

A

G

P

P

P

E

A

A

L

L

S

L

A

K

E

N

F

L

R

Q

K

M

V

V

H

H

Q

Q

Y

N

N

C

I

V

F
x
Y

T

T

V

C

N

A

T

T

P

P

V

I

Q

Q

H

E

G

A

I

I

A

A

T

V

-

G

-

F

-

E

-

T

-

E

-

L

-

K

Y

R

M

L

Q

K

N

S

P

P

A

E

P

C

Y

Y

L

F

E

N

-

S

L

I

A

S

A

G

F

E

Y

L

Q

M

R

A

K

K

R

R

D

D

L

Y

F

M

R

A

D

S

G

F

L

L

K

A

H

E

T

V

R

G

F

M

K

N

L

P

L

T

P

V

A

P

D

Q

G

G

T

G

Y

Y

F
|
F

L

M

C

V

V

A

D

D

Y

W

S

S

A

S

I

L

-

D

-

S

-

K

-

V

-

D

-

L

-

T

-

Q

-

E

-

T

S

D

A

A

M

R

S

K

E

D

A

Y

D

R

F

F

A

T

V

K

W

W

L

M

T

T

S

K

E

S

I

V

G

G

V

L

A

Q

A

G

I

I

P

P

V

P

S

S

A

A

F

F

Y

Y

D

S

S

E

P

P

R

N

E

K

-

H

-

L

-

G

S

E

G

D

I

F

V

V

R
|
R

F

Y

C

C

F

F

A

F

K

K

Q

K

D

D

Q

E

T

N

L

L

Q

Q

Q

K

A

A

L

A

Q

E

R

I

L

L

A

R

K

K

active site: F125 (≠ Y134), D211 (= D213), V213 (= V215), K245 (= K247)
binding n~2~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-glutamine: Q34 (= Q46), Y63 (= Y72), G99 (= G108), A100 (= A109), Y101 (≠ T110), F125 (≠ Y134), N179 (= N181), N183 (= N185), D211 (= D213), Y214 (= Y216), S242 (= S244), K245 (= K247), K253 (= K255), Y276 (≠ F278), F337 (= F331), R395 (= R376)

Sites not aligning to the query:

binding n~2~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-glutamine: 17

2r5cB Aedes kynurenine aminotransferase in complex with cysteine (see paper)
34% identity, 91% coverage: 35:395/396 of query aligns to 23:414/419 of 2r5cB

query
sites
2r5cB

L

L

A

A

S

A

E

Q

K

Y

S

K

A

P

V

L

N

N

L

L

G

G

Q

Q

G
|
G

F

F

P

P

D

D

F

Y

D

H

C

A

D

P

P

K

A

Y

L

A

V

L

N

N

A

A

V

L

S

A

D

A

A

A

M

A

K

N

N

S

G

P

-

D

-

P

-

L

F

A

N

N

Q

Q

Y

Y

P

T

P

R

M

G

T

F

G

G

V

H

P

P

A

R

L

L

R

V

Q

Q

A

A

I

L

A

S

A

K

K

L

I

Y

E

S

K

Q

L

L

Y

V

G

D

H

R

S

T

Y

I

D

N

P

P

A

M

T

T

D

E

I

V

T

L

V

V

T

T

A

V

G
|
G

A
|
A

T
x
Y

Q

E

G

A

L

L

L

Y

T

A

A

T

I

I

M

Q

C

G

A

H

V

V

H

D

P

E

G

G

D

D

E

E

V

V

I

I

V

I

I

I

E

E

P

P

A

F

Y
x
F

D

D

S

C

Y

Y

V

E

P

P

A

M

I

V

E

K

L

A

A

A

G

G

K

I

P

P

V

R

L

F

V

I

Q

P

M

L

-

K

-

P

-

N

-

K

-

T

-

G

T

T

L

I

G

S

A

S

D

A

G

D

Y

W

A

V

I

L

P

D

W

N

D

N

Q

E

I

L

A

E

A

A

A

L

V

F

S

N

P

E

R

K

T

T

R

K

M

M

I

I

M

I

I

I

N
|
N

S

T

P

P

H

H

N
|
N

P

P

T

L

G

G

S

K

V

V

L

M

N

D

L

R

A

A

D

E

I

L

E

E

A

V

L

V

K

A

D

N

I

L

V

C

R

K

G

K

T

W

D

N

I

V

L

L

I

C

L

V

S

S

D
|
D

E

E

V
|
V

Y
|
Y

E

E

H

H

M

M

V

V

Y

F

D

E

G

P

L

F

R

E

H

H

E

I

S

R

I

I

C

C

R

T

H

L

P

P

E

G

L

M

A

W

E

E

R

R

A

T

F

I

I

T

V

I

S

G

S
|
S

F

A

G

G

K
|
K

T

T

Y

F

H

S

V

L

T

T

G

G

W

W

K
|
K

I

I

G

G

Y

W

V

A

A

Y

A

G

P

P

P

E

A

A

L

L

S

L

A

K

E

N

F

L

R

Q

K

M

V

V

H

H

Q

Q

Y

N

N

C

I

V

F

Y

T

T

V

C

N

A

T

T

P

P

V

I

Q

Q

H

E

G

A

I

I

A

A

T

V

-

G

-

F

-

E

-

T

-

E

-

L

-

K

Y

R

M

L

Q

K

N

S

P

P

A

E

P

C

Y

Y

L

F

E

N

-

S

L

I

A

S

A

G

F

E

Y

L

Q

M

R

A

K

K

R

R

D

D

L

Y

F

M

R

A

D

S

G

F

L

L

K

A

H

E

T

V

R

G

F

M

K

N

L

P

L

T

P

V

A

P

D

Q

G

G

T

G

Y

Y

F
|
F

L

M

C

V

V

A

D

D

Y

W

S

S

A

S

I

L

-

D

-

S

-

K

-

V

-

D

-

L

-

T

-

Q

-

E

-

T

S

D

A

A

M

R

S

K

E

D

A

Y

D

R

F

F

A

T

V

K

W

W

L

M

T

T

S

K

E

S

I

V

G

G

V

L

A

Q

A

G

I

I

P

P

V

P

S

S

A

A

F

F

Y

Y

D

S

S

E

P

P

R

N

E

K

-

H

-

L

-

G

S

E

G

D

I

F

V

V

R
|
R

F

Y

C

C

F

F

A

F

K

K

Q

K

D

D

Q

E

T

N

L

L

Q

Q

Q

K

A

A

L

A

Q

E

R

I

L

L

A

R

K

K

active site: F125 (≠ Y134), D211 (= D213), V213 (= V215), K245 (= K247)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: G35 (= G47), G99 (= G108), A100 (= A109), Y101 (≠ T110), F125 (≠ Y134), N179 (= N181), N183 (= N185), D211 (= D213), Y214 (= Y216), S242 (= S244), K245 (= K247), K253 (= K255), F337 (= F331), R395 (= R376)

Sites not aligning to the query:

binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: 17

1yiyA Aedes aegypti kynurenine aminotransferase (see paper)
34% identity, 91% coverage: 35:395/396 of query aligns to 22:413/418 of 1yiyA

query
sites
1yiyA

L

L

A

A

S

A

E

Q

K

Y

S

K

A

P

V

L

N

N

L

L

G

G

Q

Q

G

G

F

F

P

P

D

D

F

Y

D

H

C

A

D

P

P

K

A

Y

L

A

V

L

N

N

A

A

V

L

S

A

D

A

A

A

M

A

K

N

N

S

G

P

-

D

-

P

-

L

F

A

N

N

Q

Q

Y

Y

P

T

P

R

M

G

T

F

G

G

V

H

P

P

A

R

L

L

R

V

Q

Q

A

A

I

L

A

S

A

K

K

L

I

Y

E

S

K

Q

L

L

Y

V

G

D

H

R

S

T

Y

I

D

N

P

P

A

M

T

T

D

E

I

V

T

L

V

V

T

T

A

V

G
|
G

A
|
A

T
x
Y

Q

E

G

A

L

L

L

Y

T

A

A

T

I

I

M

Q

C

G

A

H

V

V

H

D

P

E

G

G

D

D

E

E

V

V

I

I

V

I

I

I

E

E

P

P

A

F

Y
x
F

D

D

S

C

Y

Y

V

E

P

P

A

M

I

V

E

K

L

A

A

A

G

G

K

I

P

P

V

R

L

F

V

I

Q

P

M

L

-

K

-

P

-

N

-

K

-

T

-

G

T

T

L

I

G

S

A

S

D

A

G

D

Y

W

A

V

I

L

P

D

W

N

D

N

Q

E

I

L

A

E

A

A

A

L

V

F

S

N

P

E

R

K

T

T

R

K

M

M

I

I

M

I

I

I

N
|
N

S

T

P

P

H

H

N

N

P

P

T

L

G

G

S

K

V

V

L

M

N

D

L

R

A

A

D

E

I

L

E

E

A

V

L

V

K

A

D

N

I

L

V

C

R

K

G

K

T

W

D

N

I

V

L

L

I

C

L

V

S

S

D
|
D

E

E

V
|
V

Y
|
Y

E

E

H

H

M

M

V

V

Y

F

D

E

G

P

L

F

R

E

H

H

E

I

S

R

I

I

C

C

R

T

H

L

P

P

E

G

L

M

A

W

E

E

R

R

A

T

F

I

I

T

V

I

S

G

S
|
S

F

A

G

G

K
|
K

T

T

Y

F

H

S

V

L

T

T

G

G

W

W

K
|
K

I

I

G

G

Y

W

V

A

A

Y

A

G

P

P

P

E

A

A

L

L

S

L

A

K

E

N

F

L

R

Q

K

M

V

V

H

H

Q

Q

Y

N

N

C

I

V

F

Y

T

T

V

C

N

A

T

T

P

P

V

I

Q

Q

H

E

G

A

I

I

A

A

T

V

-

G

-

F

-

E

-

T

-

E

-

L

-

K

Y

R

M

L

Q

K

N

S

P

P

A

E

P

C

Y

Y

L

F

E

N

-

S

L

I

A

S

A

G

F

E

Y

L

Q

M

R

A

K

K

R

R

D

D

L

Y

F

M

R

A

D

S

G

F

L

L

K

A

H

E

T

V

R

G

F

M

K

N

L

P

L

T

P

V

A

P

D

Q

G

G

T

G

Y

Y

F

F

L

M

C

V

V

A

D

D

Y

W

S

S

A

S

I

L

-

D

-

S

-

K

-

V

-

D

-

L

-

T

-

Q

-

E

-

T

S

D

A

A

M

R

S

K

E

D

A

Y

D

R

F

F

A

T

V

K

W

W

L

M

T

T

S

K

E

S

I

V

G

G

V

L

A

Q

A

G

I

I

P

P

V

P

S

S

A

A

F

F

Y

Y

D

S

S

E

P

P

R

N

E

K

-

H

-

L

-

G

S

E

G

D

I

F

V

V

R

R

F

Y

C

C

F

F

A

F

K

K

Q

K

D

D

Q

E

T

N

L

L

Q

Q

Q

K

A

A

L

A

Q

E

R

I

L

L

A

R

K

K

active site: F124 (≠ Y134), D210 (= D213), V212 (= V215), K244 (= K247)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G98 (= G108), A99 (= A109), Y100 (≠ T110), F124 (≠ Y134), N178 (= N181), D210 (= D213), V212 (= V215), Y213 (= Y216), S241 (= S244), K244 (= K247), K252 (= K255)

3fvuA Crystal structure of human kynurenine aminotransferase i in complex with indole-3-acetic acid (see paper)
35% identity, 91% coverage: 35:395/396 of query aligns to 21:414/419 of 3fvuA

query
sites
3fvuA

L

L

A

A

S

S

E

E

K

H

S

D

A

V

V

V

N

N

L

L

G

G

Q

Q

G
|
G

F

F

P

P

D

D

F

F

D

P

C

P

D

P

P

D

A

F

L

A

V

V

N

E

A

A

V

F

S

Q

D

H

A

A

M

V

K

S

N

G

G

D

F

F

-

M

-

L

N

N

Q

Q

Y
|
Y

P

T

P

K

M

T

T

F

G

G

V

Y

P

P

A

P

L

L

R

T

Q

K

A

I

I

L

A
|
A

A
x
S

K

F

I

F

E

G

K

E

L

L

Y

L

G

G

H

Q

S
x
E

Y
x
I

D

D

P

P

A

L

T

R

D

N

I

V

T

L

V

V

T

T

A

V

G

G

A

G

T
x
Y

Q

G

G

A

L

L

L

F

T

T

A

A

I

F

M

Q

C

A

A

L

V

V

H

D

P

E

G

G

D

D

E

E

V

V

I

I

V

I

I

I

E

E

P

P

A

F

Y
x
F

D

D

S

C

Y

Y

V

E

P

P

A

M

I

T

E

M

L

M

A

A

G

G

K

R

P

P

V

V

L

F

V

V

Q

S

M

L

T

K

-

P

-

G

-

P

-

I

-

Q

L

N

G

G

A

E

D

L

G

G

Y

S

A

S

I

S

P

N

W

W

D

Q

-

L

-

D

-

P

-

M

Q

E

I

L

A

A

A

G

A

K

V

F

S

T

P

S

R

R

T

T

R

K

M

A

I

L

M

V

I

L

N

N

S

T

P

P

H

N

N

N

P

P

T

L

G

G

S

K

V

V

L

F

N

S

L

R

A

E

D

E

I

L

E

E

A

L

L

V

K

A

D

S

I

L

V

C

R

Q

G

Q

T

H

D

D

I

V

L

V

I

C

L

I

S

T

D
|
D

E

E

V
|
V

Y

Y

E

Q

H

W

M

M

V

V

Y

Y

D

D

G

G

L

H

R

Q

H

H

E

I

S

S

I

I

C

A

R

S

H

L

P

P

E

G

L

M

A

W

E

E

R

R

A

T

F

L

I

T

V

I

S

G

S

S

F

A

G

G

K
|
K

T

T

Y

F

H

S

V

A

T

T

G

G

W

W

K

K

I

V

G

G

Y

W

V

V

A

L

A

G

P

P

P

D

A

H

L

I

S

M

A

K

E

H

F

L

R

R

K

T

V

V

H

H

Q

Q

Y

N

N

S

I

V

F
|
F

T
x
H

V

C

N

P

T

T

P

Q

V

S

Q

Q

H

A

G

A

I

V

A

A

T

E

-

S

-

F

-

E

-

R

-

E

-

Q

-

L

Y

F

M

R

Q

Q

N

P

P

S

A

S

P

Y

Y

F

L

V

E

Q

L

F

A

P

A

Q

F

A

Y

M

Q

Q

R

R

K

C

R

R

D

D

L

H

F

M

R

I

D

R

G

S

L

L

K

Q

H

S

T

V

R

G

F

L

K

K

L

P

L

I

P

I

A

P

D

Q

G

G

T

S

Y

Y

F

F

L

L

C

I

V

T

D

D

Y

I

S

S

-

D

-

F

-

K

-

R

-

K

-

M

-

P

-

D

-

L

-

P

-

G

A

A

I

V

S

D

A

E

M

P

S

Y

E

D

A

R

D

R

F

F

A

V

V

K

W

W

L

M

T

I

S

K

E

N

I

K

G

G

V

L

A

V

A

A

I

I

P

P

V

V

S

S

A

I

F

F

Y

Y

D

S

S

V

P

P

R

H

E

Q

S

K

G

H

I

F

-

D

-

H

-

Y

V

I

R
|
R

F

F

C

C

F

F

A

V

K

K

Q

D

D

E

Q

A

T

T

L

L

Q

Q

Q

A

A

M

L

D

Q

E

R

K

L

L

A

R

K

K

active site: F122 (≠ Y134), D210 (= D213), V212 (= V215), K244 (= K247)
binding glycerol: A74 (= A86), S75 (≠ A87), E84 (≠ S96), I85 (≠ Y97)
binding 1h-indol-3-ylacetic acid: G33 (= G47), Y60 (= Y72), Y98 (≠ T110), F122 (≠ Y134), K244 (= K247), F275 (= F278), H276 (≠ T279), R395 (= R376)

Sites not aligning to the query:

binding 1h-indol-3-ylacetic acid: 15

3fvsB Human kynurenine aminotransferase i in complex with glycerol (see paper)
35% identity, 91% coverage: 35:395/396 of query aligns to 21:414/419 of 3fvsB

query
sites
3fvsB

L

L

A

A

S

S

E

E

K

H

S

D

A

V

V

V

N

N

L

L

G

G

Q

Q

G

G

F

F

P

P

D

D

F

F

D

P

C

P

D

P

P

D

A

F

L

A

V

V

N

E

A

A

V

F

S

Q

D

H

A

A

M

V

K

S

N

G

G

D

F

F

-

M

-

L

N

N

Q

Q

Y

Y

P

T

P

K

M

T

T

F

G

G

V

Y

P

P

A

P

L

L

R

T

Q

K

A

I

I

L

A

A

A

S

K

F

I

F

E

G

K

E

L

L

Y

L

G

G

H

Q

S

E

Y

I

D

D

P

P

A

L

T

R

D

N

I

V

T

L

V

V

T

T

A

V

G

G

A

G

T
x
Y

Q

G

G

A

L

L

L

F

T

T

A

A

I

F

M

Q

C

A

A

L

V

V

H

D

P

E

G

G

D

D

E

E

V

V

I

I

V

I

I

I

E

E

P

P

A

F

Y
x
F

D

D

S

C

Y

Y

V

E

P

P

A

M

I

T

E

M

L

M

A

A

G

G

K

R

P

P

V

V

L

F

V

V

Q

S

M

L

T

K

-

P

-

G

-

P

-

I

-

Q

L

N

G

G

A

E

D

L

G

G

Y

S

A

S

I

S

P

N

W

W

D

Q

-

L

-

D

-

P

-

M

Q

E

I

L

A

A

A

G

A

K

V

F

S

T

P

S

R

R

T

T

R

K

M

A

I

L

M

V

I

L

N

N

S

T

P

P

H

N

N

N

P

P

T

L

G

G

S

K

V

V

L

F

N

S

L

R

A

E

D

E

I

L

E

E

A

L

L

V

K

A

D

S

I

L

V

C

R

Q

G

Q

T

H

D

D

I

V

L

V

I

C

L

I

S

T

D
|
D

E

E

V
|
V

Y

Y

E

Q

H

W

M

M

V

V

Y

Y

D

D

G

G

L

H

R

Q

H

H

E

I

S

S

I

I

C

A

R

S

H

L

P

P

E

G

L

M

A

W

E

E

R

R

A

T

F

L

I
<