SitesBLAST
Comparing WP_061534005.1 NCBI__GCF_001584165.1:WP_061534005.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7wwxA Crystal structure of herbaspirillum huttiense l-arabinose 1- dehydrogenase (NAD bound form) (see paper)
75% identity, 96% coverage: 11:262/263 of query aligns to 2:253/254 of 7wwxA
- binding nicotinamide-adenine-dinucleotide: G18 (= G27), T21 (≠ S30), I23 (= I32), D42 (= D51), I43 (= I52), C68 (= C77), D69 (= D78), L70 (≠ I79), N96 (= N105), A98 (= A107), F146 (≠ L155), S147 (= S156), S148 (= S157), Y161 (= Y170), K165 (= K174), P191 (= P200), G192 (= G201), W193 (= W202), V194 (= V203), R198 (= R207)
5wjsA Crystal structure of oxidoreductase (short chain dehydrogenase/reductase family) from burkholderia thailandensis complexed with nadh
55% identity, 96% coverage: 11:263/263 of query aligns to 7:258/258 of 5wjsA
- active site: G27 (= G31), S152 (= S157), Y162 (= Y167), Y165 (= Y170), K169 (= K174)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G23 (= G27), T26 (≠ S30), I28 (= I32), D47 (= D51), L48 (≠ I52), D73 (= D78), L74 (≠ I79), N100 (= N105), A102 (= A107), L150 (= L155), G151 (≠ S156), S152 (= S157), K169 (= K174), P195 (= P200), G196 (= G201), W197 (= W202), V198 (= V203), K202 (≠ R207)
F1SWA0 Zerumbone synthase; EC 1.1.1.326 from Zingiber zerumbet (Shampoo ginger) (Amomum zerumbet) (see paper)
33% identity, 94% coverage: 18:263/263 of query aligns to 3:257/267 of F1SWA0
- S142 (= S157) mutation to A: Strong reduction in oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- S144 (= S159) mutation to A: Increased oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- Y155 (= Y170) mutation to A: Strong reduction in oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- K159 (= K174) mutation to A: Abolishes all oxidoreductase activity.
5ha5D Crystal structure of an NAD-bound oxidoreductase from brucella ovis
36% identity, 93% coverage: 18:262/263 of query aligns to 4:241/244 of 5ha5D
- active site: G17 (= G31), S142 (= S157), Y155 (= Y170), K159 (= K174)
- binding nicotinamide-adenine-dinucleotide: G13 (= G27), R16 (≠ S30), G17 (= G31), L18 (≠ I32), D37 (= D51), I38 (= I52), L62 (≠ C77), D63 (= D78), V64 (≠ I79), N90 (= N105), A91 (= A106), S142 (= S157), Y155 (= Y170), K159 (= K174), G186 (= G201), M188 (≠ V203), S190 (≠ T205)
5wuwA Serratia marcescens short-chain dehydrogenase/reductase f98l/f202l mutant (see paper)
36% identity, 94% coverage: 18:263/263 of query aligns to 3:244/245 of 5wuwA
- active site: G16 (= G31), S140 (= S157), Y154 (= Y170), L161 (≠ V177)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G27), R15 (≠ S30), I17 (= I32), Y36 (≠ D51), A37 (≠ I52), A38 (= A53), D63 (= D78), S64 (≠ I79), N90 (= N105), A91 (= A106), G92 (≠ A107), Y154 (= Y170), K158 (= K174), G185 (= G201), P186 (≠ W202), V187 (= V203)
2ewmB Crystal structure of the (s)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain ebn1 (see paper)
35% identity, 93% coverage: 18:261/263 of query aligns to 3:243/247 of 2ewmB
- active site: G16 (= G31), S139 (= S157), Y149 (= Y167), Y152 (= Y170), K156 (= K174)
- binding nicotinamide-adenine-dinucleotide: G12 (= G27), N15 (≠ S30), G16 (= G31), I17 (= I32), D36 (= D51), L37 (≠ I52), C59 (= C77), D60 (= D78), V61 (≠ I79), N87 (= N105), S139 (= S157), Y152 (= Y170), K156 (= K174), P182 (= P200), S183 (≠ G201), L184 (≠ W202), V185 (= V203), T189 (≠ R207)
Q5P5I4 (S)-1-Phenylethanol dehydrogenase; EC 1.1.1.311 from Aromatoleum aromaticum (strain DSM 19018 / LMG 30748 / EbN1) (Azoarcus sp. (strain EbN1)) (see 2 papers)
35% identity, 93% coverage: 18:261/263 of query aligns to 5:245/249 of Q5P5I4
- NGI 17:19 (≠ SGI 30:32) binding NAD(+)
- D38 (= D51) binding NAD(+)
- CDV 61:63 (≠ CDI 77:79) binding NAD(+)
- N89 (= N105) binding NAD(+)
- Y93 (≠ D109) binding NAD(+)
- K158 (= K174) binding NAD(+)
- PSLV 184:187 (≠ PGWV 200:203) binding NAD(+)
- T191 (≠ R207) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
6j7uA Crystal structure of blue fluorescent protein from metagenomic library in complex with NADPH (see paper)
36% identity, 94% coverage: 17:263/263 of query aligns to 2:246/247 of 6j7uA
- active site: G16 (= G31), S142 (= S157), Y156 (= Y170)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G27), S14 (≠ G29), R15 (≠ S30), I17 (= I32), Y36 (≠ D51), V37 (≠ I52), S38 (≠ A53), S41 (≠ A56), D65 (= D78), S66 (≠ I79), N92 (= N105), A93 (= A106), G94 (≠ A107), I115 (= I128), G141 (≠ S156), S142 (= S157), Y156 (= Y170), K160 (= K174), P186 (= P200), T191 (= T205), M193 (≠ R207), N194 (≠ Q208)
8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
32% identity, 93% coverage: 18:261/263 of query aligns to 3:247/251 of 8cxaA
- binding nicotinamide-adenine-dinucleotide: G12 (= G27), Q15 (≠ S30), G16 (= G31), I17 (= I32), D36 (= D51), V63 (≠ I79), N89 (= N105), A91 (= A107), S94 (vs. gap), I142 (≠ L155), S143 (= S156), S144 (= S157), Y157 (= Y170), K161 (= K174), P187 (= P200), H188 (≠ G201), I190 (≠ V203), I194 (≠ L211)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
31% identity, 95% coverage: 13:262/263 of query aligns to 1:244/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G27), S17 (≠ G29), R18 (≠ S30), I20 (= I32), T40 (≠ I52), N62 (≠ D78), V63 (≠ I79), N89 (= N105), A90 (= A106), I92 (≠ N108), V139 (≠ L155), S141 (= S157), Y154 (= Y170), K158 (= K174), P184 (= P200), G185 (= G201), I187 (≠ V203), T189 (= T205), M191 (≠ R207)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
32% identity, 93% coverage: 17:261/263 of query aligns to 2:236/239 of 4nbtA
- active site: G16 (= G31), S132 (= S157), Y145 (= Y170), K149 (= K174)
- binding nicotinamide-adenine-dinucleotide: G12 (= G27), K15 (≠ S30), G16 (= G31), L17 (≠ I32), D36 (= D51), L37 (= L60), L52 (≠ C77), N53 (≠ D78), V54 (≠ I79), N80 (= N105), A81 (= A106), G82 (≠ A107), I130 (≠ L155), S132 (= S157), Y145 (= Y170), K149 (= K174), P177 (= P200), G178 (= G201), I180 (≠ V203), T182 (= T205)
3o4rA Crystal structure of human dehydrogenase/reductase (sdr family) member 4 (dhrs4)
33% identity, 93% coverage: 18:261/263 of query aligns to 6:249/254 of 3o4rA
- active site: G19 (= G31), S145 (= S157), F155 (≠ Y167), Y158 (= Y170), K162 (= K174), K203 (≠ A215)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: A15 (≠ G27), T17 (≠ G29), D18 (≠ S30), G19 (= G31), I20 (= I32), S39 (vs. gap), R40 (vs. gap), K41 (vs. gap), N44 (vs. gap), H65 (≠ D78), V66 (≠ I79), N92 (= N105), A94 (= A107), S145 (= S157), Y158 (= Y170), K162 (= K174), P188 (= P200), G189 (= G201), L190 (≠ W202), I191 (≠ V203), T193 (= T205), F195 (≠ R207), S196 (≠ Q208)
Q9BTZ2 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase; CR; NADPH-dependent retinol dehydrogenase/reductase; NRDR; humNRDR; Peroxisomal short-chain alcohol dehydrogenase; PSCD; SCAD-SRL; Short chain dehydrogenase/reductase family 25C member 2; Protein SDR25C2; Short-chain dehydrogenase/reductase family member 4; EC 1.1.1.184 from Homo sapiens (Human) (see 2 papers)
33% identity, 93% coverage: 18:261/263 of query aligns to 30:273/278 of Q9BTZ2
- S176 (≠ N164) Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin; mutation to F: Decreased reduction activity for benzil, isatin and retinal and increased activity for 5beta-Pregnane-3,20-dione and 5beta-Dihydrotestosterone. No change of stereoselectivity in 3-ketosteroids reduction and no change in 3beta-hydroxysteroid oxidation. Decreased reduction activity for isatin and increased activity for 5beta-Pregnane-3,20-dione, 5beta-Dihydrotestosterone, benzil and retinal; when associated with L-179. Change in stereoselective activity by the reduction of 5beta-Pregnane-3,20-dione predominantly to the 3alpha-hydroxysteroid; when associated with L-179. Switch from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with L-179. Loss of cold catalytic inactivation; when associated with L-179 and N-195. Increased reduction activity for renital and oxidation activity for retinol; when associated with L-179 and N-195.
- F179 (≠ Y167) Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin; mutation to L: Decreased reduction activity for isatin and increased activity for 5beta-Pregnane-3,20-dione, 5beta-Dihydrotestosterone, benzil and retinal; when associated with F-176. Change in stereoselective activity by the reduction of 5beta-Pregnane-3,20-dione predominantly to the 3alpha-hydroxysteroid; when associated with F-176. Switch from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with F-176. Loss of cold catalytic inactivation; when associated with F-176 and N-195. Increased reduction activity for renital and oxidation activity for retinol; when associated with F-176 and N-195.
- T195 (≠ G183) mutation to N: Loss of cold catalytic inactivation. Loss of cold catalytic inactivation; when associated with F-176 and L-179. Switch in stereoselective activity from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with F-176 and L-179. Increased reduction activity for renital and oxidation activity for retinol; when associated with F-176 and L-179.
2ztlA Closed conformation of d-3-hydroxybutyrate dehydrogenase complexed with NAD+ and l-3-hydroxybutyrate (see paper)
31% identity, 93% coverage: 18:262/263 of query aligns to 2:257/260 of 2ztlA
- active site: G15 (= G31), N114 (= N129), S142 (= S157), Y155 (= Y170), K159 (= K174), L200 (≠ W212)
- binding (3s)-3-hydroxybutanoic acid: Q94 (≠ D109), S142 (= S157), H144 (≠ S159), K152 (≠ Y167), Y155 (= Y170), Q196 (= Q208)
- binding nicotinamide-adenine-dinucleotide: G11 (= G27), G15 (= G31), I16 (= I32), F36 (≠ I52), L64 (≠ I79), N90 (= N105), A91 (= A106), G92 (≠ A107), L113 (≠ I128), Y155 (= Y170), K159 (= K174), P185 (= P200), W187 (= W202), V188 (= V203), T190 (= T205), V193 (vs. gap)
1wmbA Crystal structure of NAD dependent d-3-hydroxybutylate dehydrogenase (see paper)
31% identity, 93% coverage: 18:262/263 of query aligns to 2:257/260 of 1wmbA
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
34% identity, 93% coverage: 18:261/263 of query aligns to 2:241/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G27), M16 (≠ I32), D35 (= D51), I36 (= I52), I62 (= I79), N88 (= N105), G90 (≠ A107), I138 (≠ L155), S140 (= S157), Y152 (= Y170), K156 (= K174), I185 (≠ V203)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
34% identity, 93% coverage: 18:261/263 of query aligns to 3:242/248 of Q9KJF1