SitesBLAST
Comparing WP_061534238.1 NCBI__GCF_001584165.1:WP_061534238.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5wuwA Serratia marcescens short-chain dehydrogenase/reductase f98l/f202l mutant (see paper)
67% identity, 96% coverage: 10:252/252 of query aligns to 3:245/245 of 5wuwA
- active site: G16 (= G23), S140 (= S147), Y154 (= Y161), L161 (= L168)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G19), R15 (= R22), I17 (= I24), Y36 (= Y43), A37 (≠ I44), A38 (≠ S45), D63 (= D70), S64 (= S71), N90 (= N97), A91 (= A98), G92 (= G99), Y154 (= Y161), K158 (= K165), G185 (= G192), P186 (= P193), V187 (= V194)
6j7uA Crystal structure of blue fluorescent protein from metagenomic library in complex with NADPH (see paper)
54% identity, 97% coverage: 8:252/252 of query aligns to 1:247/247 of 6j7uA
- active site: G16 (= G23), S142 (= S147), Y156 (= Y161)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G19), S14 (= S21), R15 (= R22), I17 (= I24), Y36 (= Y43), V37 (≠ I44), S38 (= S45), S41 (≠ E48), D65 (= D70), S66 (= S71), N92 (= N97), A93 (= A98), G94 (= G99), I115 (= I120), G141 (= G146), S142 (= S147), Y156 (= Y161), K160 (= K165), P186 (= P191), T191 (= T196), M193 (= M198), N194 (= N199)
5u2wA Crystal structure of a short chain dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP
56% identity, 95% coverage: 10:249/252 of query aligns to 4:243/246 of 5u2wA
- active site: G17 (= G23), S141 (= S147), M152 (≠ G158), Y155 (= Y161), K159 (= K165)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G19), S15 (= S21), R16 (= R22), G17 (= G23), I18 (= I24), Y37 (= Y43), E38 (≠ I44), K39 (≠ S45), S40 (= S46), A63 (= A69), D64 (= D70), S65 (= S71), N91 (= N97), A92 (= A98), G93 (= G99), T139 (≠ I145), Y155 (= Y161), K159 (= K165), P185 (= P191), G186 (= G192), T188 (≠ V194), T190 (= T196), M192 (= M198), N193 (= N199)
P39333 Cyclic-di-GMP-binding biofilm dispersal mediator protein from Escherichia coli (strain K12) (see paper)
50% identity, 96% coverage: 10:252/252 of query aligns to 4:237/237 of P39333
- E50 (≠ D56) mutation E->Q,V: Shows higher affinity for cyclic-di-GMP, increases swimming motility and biofilm dispersal. Biofilm formation is almost completely removed.
5z2lK Crystal structure of bdca in complex with NADPH (see paper)
50% identity, 96% coverage: 10:252/252 of query aligns to 3:236/244 of 5z2lK
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S14 (= S21), R15 (= R22), I17 (= I24), Y36 (= Y43), A37 (≠ I44), G38 (≠ S45), S39 (= S46), T57 (≠ A69), D58 (= D70), S59 (= S71), N81 (= N97), A82 (= A98), G83 (= G99), I129 (= I145), S131 (= S147), Y145 (= Y161), K149 (= K165), P175 (= P191), I178 (≠ V194), T180 (= T196), A182 (≠ M198), N183 (= N199)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
40% identity, 96% coverage: 10:250/252 of query aligns to 3:245/247 of 4jroC
- active site: G16 (= G23), S142 (= S147), Q152 (≠ G158), Y155 (= Y161), K159 (= K165)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G19), S14 (= S21), R15 (= R22), G16 (= G23), I17 (= I24), N35 (≠ T42), Y36 (= Y43), N37 (≠ I44), G38 (≠ S45), S39 (= S46), N63 (≠ D70), V64 (≠ S71), N90 (= N97), A91 (= A98), I93 (≠ V100), I113 (= I120), S142 (= S147), Y155 (= Y161), K159 (= K165), P185 (= P191), I188 (≠ V194), T190 (= T196)
4fj1B Crystal structure of the ternary complex between a fungal 17beta- hydroxysteroid dehydrogenase (holo form) and genistein (see paper)
38% identity, 95% coverage: 10:249/252 of query aligns to 5:257/259 of 4fj1B
- active site: G18 (= G23), S142 (= S147), N143 (≠ T148), H153 (≠ G158), Y156 (= Y161), K160 (= K165), Y201 (vs. gap)
- binding genistein: G188 (≠ P193), F194 (≠ N199), S198 (vs. gap), Y201 (vs. gap), I202 (vs. gap), M216 (= M213), A217 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G19), R17 (= R22), G18 (= G23), I19 (= I24), A39 (≠ I44), N40 (≠ S45), S41 (= S46), I66 (≠ S71), N92 (= N97), S93 (≠ A98), G94 (= G99), L115 (≠ I120), T140 (≠ I145), S142 (= S147), Y156 (= Y161), K160 (= K165), G187 (= G192), T189 (≠ V194), T191 (= T196), M193 (= M198)
4fj0D Crystal structure of the ternary complex between a fungal 17beta- hydroxysteroid dehydrogenase (holo form) and 3,7-dihydroxy flavone (see paper)
38% identity, 95% coverage: 10:249/252 of query aligns to 7:259/261 of 4fj0D
- active site: G20 (= G23), S144 (= S147), N145 (≠ T148), H155 (≠ G158), Y158 (= Y161), K162 (= K165), Y203 (vs. gap)
- binding 3,7-dihydroxy-2-phenyl-4H-chromen-4-one: S144 (= S147), N145 (≠ T148), G190 (≠ P193), F196 (≠ N199), S200 (vs. gap), Y203 (vs. gap), A219 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G19), R19 (= R22), G20 (= G23), I21 (= I24), A41 (≠ I44), N42 (≠ S45), S43 (= S46), I68 (≠ S71), N94 (= N97), S95 (≠ A98), G96 (= G99), L117 (≠ I120), T142 (≠ I145), Y158 (= Y161), K162 (= K165), P188 (= P191), G189 (= G192), G190 (≠ P193), T191 (≠ V194), T193 (= T196), M195 (= M198)
4fj2B Crystal structure of the ternary complex between a fungal 17beta- hydroxysteroid dehydrogenase (holo form) and biochanin a (see paper)
38% identity, 95% coverage: 10:249/252 of query aligns to 6:258/260 of 4fj2B
- active site: G19 (= G23), S143 (= S147), N144 (≠ T148), H154 (≠ G158), Y157 (= Y161), K161 (= K165), Y202 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G19), R18 (= R22), G19 (= G23), I20 (= I24), A40 (≠ I44), N41 (≠ S45), S42 (= S46), I67 (≠ S71), N93 (= N97), S94 (≠ A98), G95 (= G99), L116 (≠ I120), T141 (≠ I145), Y157 (= Y161), K161 (= K165), G188 (= G192), G189 (≠ P193), T190 (≠ V194), T192 (= T196), M194 (= M198)
- binding 5,7-dihydroxy-3-(4-methoxyphenyl)-4H-chromen-4-one: G189 (≠ P193), F195 (≠ N199), V198 (vs. gap), S199 (vs. gap), Y202 (vs. gap), I203 (vs. gap), M217 (= M213), A218 (vs. gap)
3qwiA Crystal structure of a 17beta-hydroxysteroid dehydrogenase (holo form) from fungus cochliobolus lunatus in complex with NADPH and coumestrol (see paper)
38% identity, 95% coverage: 10:249/252 of query aligns to 6:258/260 of 3qwiA
- active site: G19 (= G23), S143 (= S147), N144 (≠ T148), H154 (≠ G158), Y157 (= Y161), K161 (= K165), Y202 (vs. gap)
- binding Coumestrol: F149 (≠ M153), G189 (≠ P193), M194 (= M198), Y202 (vs. gap), I203 (vs. gap), A218 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G19), R18 (= R22), I20 (= I24), A40 (≠ I44), N41 (≠ S45), S42 (= S46), I67 (≠ S71), N93 (= N97), S94 (≠ A98), G95 (= G99), L116 (≠ I120), T141 (≠ I145), Y157 (= Y161), K161 (= K165), P187 (= P191), G188 (= G192), G189 (≠ P193), T190 (≠ V194), T192 (= T196), M194 (= M198)
3qwhA Crystal structure of the 17beta-hydroxysteroid dehydrogenase from cochliobolus lunatus in complex with NADPH and kaempferol (see paper)
38% identity, 95% coverage: 10:249/252 of query aligns to 6:258/260 of 3qwhA
- active site: G19 (= G23), S143 (= S147), N144 (≠ T148), H154 (≠ G158), Y157 (= Y161), K161 (= K165), Y202 (vs. gap)
- binding 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4h-chromen-4-one: N144 (≠ T148), F149 (≠ M153), G189 (≠ P193), F195 (≠ N199), S199 (vs. gap), Y202 (vs. gap), I203 (vs. gap), A218 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G19), R18 (= R22), G19 (= G23), I20 (= I24), A40 (≠ I44), N41 (≠ S45), S42 (= S46), D66 (= D70), I67 (≠ S71), N93 (= N97), S94 (≠ A98), G95 (= G99), L116 (≠ I120), T141 (≠ I145), Y157 (= Y161), K161 (= K165), P187 (= P191), G188 (= G192), G189 (≠ P193), T190 (≠ V194), T192 (= T196), M194 (= M198)
7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
38% identity, 98% coverage: 3:250/252 of query aligns to 2:252/253 of 7v0hG
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G19), S20 (= S21), K21 (≠ R22), G22 (= G23), I23 (= I24), A43 (≠ I44), S44 (= S45), S45 (= S46), G68 (≠ A69), D69 (= D70), V70 (≠ S71), N96 (= N97), S97 (≠ A98), G98 (= G99), Y100 (≠ L101), I144 (= I145), S146 (= S147), Y159 (= Y161), K163 (= K165), P189 (= P191), G190 (= G192), M191 (≠ P193), I192 (≠ V194), T194 (= T196), G196 (≠ M198), T197 (≠ N199)
- binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S147), Y159 (= Y161), M191 (≠ P193), I202 (vs. gap)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
38% identity, 94% coverage: 13:250/252 of query aligns to 5:244/246 of 3osuA
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
39% identity, 96% coverage: 10:250/252 of query aligns to 4:244/247 of P73574
- A14 (≠ G20) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ A156) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K165) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ P193) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ S203) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
37% identity, 94% coverage: 13:250/252 of query aligns to 2:237/239 of 3sj7A
- active site: G12 (= G23), S138 (= S147), Q148 (vs. gap), Y151 (= Y161), K155 (= K165)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G19), S10 (= S21), R11 (= R22), I13 (= I24), N31 (≠ T42), Y32 (= Y43), A33 (≠ I44), G34 (≠ S45), S35 (= S46), A58 (= A69), N59 (≠ D70), V60 (≠ S71), N86 (= N97), A87 (= A98), T109 (≠ I120), S138 (= S147), Y151 (= Y161), K155 (= K165), P181 (= P191), G182 (= G192)
8hfkA Crystal structure of cbar mutant (h162f) in complex with NADP+ and halogenated aryl ketone (see paper)
37% identity, 95% coverage: 10:249/252 of query aligns to 6:257/259 of 8hfkA