SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_061534265.1 NCBI__GCF_001584165.1:WP_061534265.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 18 hits to proteins with known functional sites (download)

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 72% coverage: 22:334/436 of query aligns to 86:399/534 of P53322

query
sites
P53322

K

R

K

K

V

M

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D

W

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Y

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I

K283 (≠ E221) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

8u3hA Structure of fmoc-leu-oh bound sialin (see paper)
24% identity, 89% coverage: 36:424/436 of query aligns to 15:409/425 of 8u3hA

query
sites
8u3hA

Y

F

V

F

V

I

A

V

Y
|
Y

L

A

D

L

R

R

V

V

N

N

V

L

G

S

F

V

A

A

K

L

L

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D

M

M

-

V

-

D

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Y

G

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F

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Y

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Y

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E

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N

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G

G

A

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I

I

binding leucine: M279 (= M304), S344 (= S360)
binding Fluorenylmethyloxycarbonyl chloride: Y19 (= Y40), H235 (≠ Y260), Y238 (≠ I263), Y243 (= Y268)

8u3gA Structure of naag-bound sialin (see paper)
24% identity, 89% coverage: 36:424/436 of query aligns to 15:411/427 of 8u3gA

query
sites
8u3gA

Y
x
F

V

F

V

I

A

V

Y
|
Y

L

A

D

L

R

R

V

V

N

N

V

L

G

S

F

V

A

A

K

L

L

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Q

D

M

M

S

V

Q

D

D

S

L

T

G

G

-

K

-

Y

-

Q

F

W

S

D

E

A

T

E

V

T

F

Q

G

G

L

W

G

I

A

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G

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F

F

L

Y

G

G

Y

Y

F

I

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N

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A

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A

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F

L

Y

I

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V

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R

F

A

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L

E

G

G

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A

G

E

E

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G

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F

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Y
x
F

P

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A

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F

Y

L

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A

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F

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A

C

M

M

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I

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L

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G

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Q

R

A

S

A

D

D

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N

L

L

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E

A

R

-

R

K

W

W

H

N

L

F

I

S

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T

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L

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C

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V

-

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F

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S

L

L

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G

G

A

M

I

I

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G

F

P

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A

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S

F

A

L

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A

A

D

G

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F

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I

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G

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N

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Y

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A

A

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I

A

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F

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A

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M

T

G

L

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G

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F

C

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G

F

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W

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S
|
S

L

I

P

N

T
x
H

A

L

F

D

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S

A

G

P

T

S

G

Y

A

A

A

G

A

I

G

L

I
x
L

A

G

V

I

I

T

N

N

S

T

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F

G

A

N

T

L

I

A

P

G

G

F

M

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G

P

P

F

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L

I

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A

G

-

W

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K

D

S

T

L

T

T

H

P

N

D

N

N

E

T

T

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G

G

-

E

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Q

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T

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M

F

F

Y

M

I

L

A

A

A

G

A

M

I

L

N

V

V

V

F

G

G

A

A

I

I

binding aspartic acid: F15 (≠ Y36), Y19 (= Y40), F118 (≠ Y133)
binding glutamic acid: S350 (= S365), H353 (≠ T368), L365 (≠ I380)

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
24% identity, 82% coverage: 69:424/436 of query aligns to 113:472/495 of Q9NRA2

query
sites
Q9NRA2

G

G

I

S

F

F

L

Y

G

G

Y

Y

F

I

L

I

F

T

E

Q

L

I

P

P

S

G

N

G

L

Y

L

V

M

A

N

S

K

K

V

I

G

G

A

G

K
|
K

L

M

W

-

I

-

A

-

R

-

I

-

M

L

I

L

T

G

W

F

G

G

I

I

L

L

S

G

A

T

C

A

F

V

A

L

W

T

V

L

Q

F

T

T

P

P

T

I

-

A

-

D

-

L

-

G

-

V

-

G

Q

P

F

L

Y

I

V

V

L

L

R

R

F

A

L

L

L

E

G

G

L

L

A

G

E

E

A

G

G

V

F

T

Y

F

P

P

G

A

I

M

I
x
H

L

A

Y

M

L

W

T

S

Y

S

W

W

F

A

P

P

S

P

H

L

R

E

R

R

A

S

K

K

V
x
L

A

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T

I

F

S

M

Y

A

A

A

G

I

A

P

Q

I

L

A

G

G

T

I

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F

I

G

S

N

L

P

P

L

L

S

S

G

G

W

I

I

I

M

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Q

Y

A

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F

-

H

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G

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S

-

G

-

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Y

H

M

G

N

W

W

Q

T

W

Y

M

V

F

F

M

Y

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G

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I

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D

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K

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K

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Y

E

E

K

K

D

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Y

L
x
I

E
x
L

A
x
S

E
x
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x
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R

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-

D
x
N

Q

Q

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L

D

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K

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S

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A

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P

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G

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A

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A

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A

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-

F

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N

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A

A

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A

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F

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S

A

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T

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L

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G

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G

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-

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A

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A

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P

T

S

G

Y

A

A

A

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A

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A

G

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I

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N

N

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A

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G

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A

G

-

W

-

L

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K

D

S

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L

T

T

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N

D

N

N

E

T

T

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G

G

-

E

-

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-

Q

-

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-

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F

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Y

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I

K136 (= K92) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ I137) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ VV 152:153) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ LE 227:228) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLR-N 268:272 (≠ AEIRAD 229:234) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G290) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P296) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G328) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
21% identity, 85% coverage: 32:403/436 of query aligns to 21:397/430 of P0AA76

query
sites
P0AA76

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Y29 (= Y40) binding D-galactonate
D31 (= D42) mutation to N: Loss of galactonate transport activity.
R32 (= R43) binding D-galactonate
Y64 (= Y75) binding D-galactonate
E118 (= E129) mutation to Q: Loss of galactonate transport activity.
W358 (= W364) binding D-galactonate

6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
22% identity, 85% coverage: 32:403/436 of query aligns to 10:378/409 of 6e9nA

query
sites
6e9nA

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x
F

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A

A

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-

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P

A

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G

A

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I

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G

A

A

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N

A

Y

A

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N

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A

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A

L

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A

A

A

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N

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-

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A

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G

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N
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N

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binding D-gluconic acid: F111 (≠ Y133), N359 (= N384)

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
23% identity, 85% coverage: 32:403/436 of query aligns to 13:362/393 of 6e9oA

query
sites
6e9oA

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x
F

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A

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x
Q

P

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A

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Q

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A

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F

M

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A

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G

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V

D

Y

N

Q

S

P

I

P

R

R

K

L

A

T

K

K

W

G

L

I

T

S

E

K

S

A

E

E

K

L

D

D

F

Y

L

I

E

-

A

-

E

-

I

-

R

-

A

-

D

-

Q

-

K

R

D

D

K

G

H

G

S

G

P

L

K

V

S

L

T

T

A

A

A

K

V

D

F

W

K

K

D

-

I

-

R

L

I

V

W

F

H

H

M

R

C

K

L

L

I

I

Y

-

F

-

C

G

I

V

V

Y

M

L

G

G

Q
|
Q

Y

F

G

A

L

V

-

A

-

S

T

T

F

L

W

W

L

F

P

F

T

L

L

T

V

L

K

K

A

A

S

-

G

-

V

-

G

-

D

-

F

-

R

-

I

-

G

G

L

F

I

M

S

T

A

T

I

V

P

P

F

F

L

L

C

A

A

A

V

F

F

V

A

G

M

V

I

L

L

L

I

S

G

G

R

W

R

V

S

A

D

D

R

L

L

L

R

V

E

R

R

K

R

G

W

F

H

S

L

L

-

G

-

F

-

A

-

R

I

K

V

T

P

P

A

I

L

I

L

C

G

G

A

L

I

L

-

I

-

S

G

T

F

C

V

I

V

M

S

G

A

A

I

N

A

Y

A

T

D

N

N

D

T

P

V

M

I

M

A

I

I

M

I

C

F

L

L

M

S

A

L

L

A

A

A

F

M

F

G

G

V

N

L

G

T

F

C

A

S
|
S

P

-

L

I

F

T

W

W

S

S

L

L

-

V

-

S

-

L

-

A

P

P

T

M

A

R

F

L

L

I

S

G

G

L

T

T

G

G

A

-

G

-

I

-

A

G

V

V

I

F

N
|
N

S

F

V

A

G

G

N

G

L

L

A

G

G

G

F

I

V

T

S

V

P

P

F

L

L

V

V

V

G

G

W

Y

L

L

K

A

D

Q

binding D-galactonic acid: Y21 (= Y40), F114 (≠ Y133), Q141 (≠ I160), Q228 (= Q267), S320 (= S360), N343 (= N384)

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
22% identity, 82% coverage: 69:424/436 of query aligns to 113:472/495 of Q8BN82

query
sites
Q8BN82

G

G

I

S

F

F

L

Y

G

G

Y

Y

F

I

L

V

F

T

E

Q

L

I

P

P

S

G

N

G

L

Y

L

I

M

A

N

S

K

R

V

V

G

G

A

G

K

K

L

L

W

L

I

L

A

G

R

L

I

G

M

I

I

L

T

G

W

T

G

S

I

V

L

F

S

T

A

-

C

-

F

-

A

-

W

-

V

L

Q

F

T

T

P

P

-

L

-

A

-

D

-

L

-

G

-

V

T

V

Q

T

F

L

Y

V

V

V

L

L

R

R

F

A

L

L

L

E

G

G

L

L

A

G

E

E

A

G

G

V

F

T

Y

F

P

P

G

A

I

M

I
x
H

L

A

Y

M

L

W

T

S

Y

S

W

W

F

A

P

P

S

P

H

L

R

E

R

R

A

S

K

K

V

L

A

T

T

I

F

S

M

Y

A

A

A

G

I

A

P

Q

I

L

A

G

G

T

I

V

F

I

G

S

N

L

P

P

L

L

S

S

G

G

W

I

I

I

M

C

Q

Y

A

-

F

-

H

-

G

-

S

-

G

-

W

Y

H

M

G

N

W

W

Q

T

W

Y

M

V

F

F

M

Y

I

L

E

F

A

G

I

I

P

V

A

G

I

I

L

V

V

W

G

F

I

I

A

L

V

W

V

M

F

W

V

I

M

V

D

S

N

D

S

T

I

P

R

E

K

T

A

H

K

K

W

T

L

I

T

S

E

H

S

Y

E

E

K

K

D

E

F

Y

L

I

E

V

A

S

E

S

I

L

R

K

A

-

D

N

Q

Q

K

L

D

S

K

S

H

Q

S

K

P

V

K

V

S

P

T

W

A

G

A

S

V

I

F

L

K

K

D

S

I

L

R

P

I

L

W

W

H

A

M

I

C

V

L

V

I

A

Y

H

F

F

C

S

I

Y

V

N

M

W

G

S

Q

F

Y

Y

G

T

L

L

T

L

F

T

W

L

L

L

P

P

T

T

L

Y

V

M

K

K

A

E

S

I

G

L

V

R

V

F

G

N

D

V

F

Q

R

E

I

N

G

G

L

F

I

L

S

S

A

A

I

L

P

P

F

Y

L

F

C

G

A

C

V

W

F

L

A

C

M

M

I

I

L

L

I

C

G

G

R

Q

R

A

S

A

D

D

R

Y

L

L

R

R

E

-

R

V

R

K

W

W

H

N

L

F

I

S

-

T

-

I

-

S

-

V

-

R

V

I

P

F

A

S

L

L

L

V

G

G

A

M

I

V

G

G

F

P

V

A

V

V

S

F

A

L

I

V

A

A

D

G

-

F

-

I

-

G

-

C

N

D

T

Y

V

S

I

L

A

A

I

V

I

A

F

F

L

L

S

T

L

I

A

S

-

T

A

T

M

L

G

G

V

G

L

F

T

A

C

S

S

S

P

G

L

F

F

-

W

-

S

S

L

I

P

N

T

H

A

L

F

D

L

I

S

A

G

P

T

S

G

Y

A

A

A

G

A

I

G

L

I

L

A

G

V

I

I

T

N

N

S

T

V

F

G

A

N

T

L

I

A

P

G

G

F

M

V

T

S

G

P

P

F

I

L

I

V

A

G

K

W

S

L

L

-

T

-

P

K

D

D

N

T

T

T

I

H

R

N

E

N

W

E

Q

T

T

G

V

M

F

F

C

M

I

L

A

A

A

G

A

M

I

L

N

V

V

V

F

G

G

A

A

I

I

H183 (≠ I137) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 70% coverage: 120:424/436 of query aligns to 166:472/495 of Q5Q0U0

query
sites
Q5Q0U0

V

V

L

L

R
|
R

F

A

L

L

L
x
E

G
|
G

L

L

A

G

E
|
E

A
x
G

G

V

F

T

Y
x
F

P
|
P

G

A

I

M

I
x
H

L

A

Y

M

L
x
W

T

S

Y

S

W

W

F

A

P

P

S

P

H

L

R

E

R

R

A

S

K

K

V

L

A

T

T

I

F

S

M

Y

A

A

A

G

I

A

P

Q

I

L

A

G

G

T

I

V

F

I

G

S

N

L

P

P

L

L

S

S

G

G

W

I

I

I

M

C

Q

Y

A

-

F

-

H

-

G

-

S

-

G

-

W

Y

H

M

G

N

W

W

Q

T

W

Y

M

V

F

F

M

Y

I

L

E

F

A

G

I

L

P

V

A

G

I

I

L

V

V

W

G

F

I

I

A

L

V

W

V

M

F

W

V

I

M

V

D

S

N

D

S

T

I

P

R

E

K

T

A

H

K

K

W

T

L

I

T

S

E

H

S

Y

E

E

K

K

D

E

F

Y

L

I

E

V

A
x
S

E
x
S

I
x
L

R
x
K

A

-

D
x
N

Q

Q

K

L

D

S

K

S

H

Q

S

K

P

V

K

V

S

P

T

W

A

V

A

S

V

I

F

L

K

K

D

S

I

L

R

P

I

L

W

W

H

A

M

I

C

V

L

V

I

A

Y

H

F

F

C

S

I

Y

V

N

M

W

G

T

Q

F

Y

Y

G

T

L

L

T

L

F

T

W

L

L

L

P

P

T

T

L

Y

V

M

K

K

A

E

S

I

G

L

V

R

V

F

G

N

D

V

F

Q

R

E

I

N

G

G

L

F

I

L

S

S

A

A

I

L

P
|
P

F

Y

L

F

C

G

A

C

V

W

F

L

A

C

M

M

I

I

L

V

I

C

G

G

R

Q

R

A

S

A

D

D

R

Y

L

L

R

R

E

-

R

V

R

K

W

W

H

N

L

F

I

S

-

T

-

I

-

S

-

V

-

R

V

I

P

F

A

S

L

L

L

I

G
|
G

A

M

I

V

G

G

F

P

V

A

V

V

S

F

A

L

I

V

A

A

D

G

-

F

-

I

-

G

-

C

N

D

T

Y

V

S

I

L

A

A

I

V

I

A

F

F

L

L

S

T

L

I

A

S

-

T

A

T

M

L

G

G

V

G

L

F

T

A

C

S

S

S

P

G

L

F

F

-

W

-

S

S

L

I

P

N

T

H

A

L

F

D

L

I

S

A

G

P

T

S

G

Y

A

A

A

G

A

I

G

L

I

L

A

G

V

I

I

T

N

N

S

T

V

F

G

A

N

T

L

I

A

P

G

G

F

M

V

I

S

G

P

P

F

I

L

I

V

A

G

K

W

S

L

L

-

T

-

P

K

D

D

N

T

T

T

I

H

K

N

E

N

W

E

Q

T

T

G

V

M

F

F

C

M

I

L

A

A

A

G

A

M

I

L

N

V

V

V

F

G

G

A

A

I

I

R168 (= R122) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L125) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G126) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E129) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A130) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (≠ Y133) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P134) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ I137) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ L140) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLK-N 268:272 (≠ AEIRAD 229:234) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P296) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (= G328) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
136 K→E: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
22% identity, 86% coverage: 15:388/436 of query aligns to 20:398/448 of Q51955

query
sites
Q51955

E

Q

Q

Q

A

P

E

L

N

S

L

R

Y

Y

K

Q

K

-

V

-

F

-

W

W

R

R

F

V

V

V

P

-

F

-

I

-

M

L

L

L

C

C

Y

F

V

L

V

I

A

V

Y

F

L

L

D
|
D

R

G

V

L

N
x
D

-

T

-

A

V

M

G

G

F

F

A

I

K

A

L

P

Q

A

M

L

S

S

Q

Q

D

E

L

W

G

G

F

I

S

D

E

R

T

A

V

S

F

L

G

G

L

P

G

V

A

M

G

S

I

A

F

A

F

L

L

I

G

G

Y

M

F

V

L

F

F

G

E

A

L

L

P

G

S

S

N
x
G

L

P

L

L

M

A

N
x
D

K

R

V

F

G
|
G

A

R

K

K

L

G

W

V

I

L

A

V

R

G

I

A

M

V

I

L

T

V

W

F

G

G

I

G

L

F

S

S

A

L

C

A

F

S

A

A

W

Y

V

A

Q

T

T

N

P

V

T

D

Q

Q

F

L

Y

L

V

V

L

L

R
|
R

F

F

L

L

L

T

G

G

L

L

A

G

E

L

A

G

G

A

F

G

Y

M

P

P

G

N

I

A

I

T

L

T

Y

L

L

L

T

S

Y
x
E

W

Y

F

T

P

P

S

E

H

R

R

L

R

K

A

S

K

L

V

L

V

V

A

T

T

S

F

M

M

F

A

C

A

G

I

F

P

N

I

L

A

G

G

M

I

A

F

G

G

G

N

G

P

F

L

I

S

S

G

A

W

K

I

M

M

I

Q

P

A

A

F

Y

H

-

G

-

S

-

G

-

W

-

H

-

G

G

W

W

Q
x
H

W

S

M

L

F

L

M

V

I

I

E

G

A

G

I

V

P

L

A

P

I

L

L

L

V

L

G

A

I

L

A

V

V

L

V

M

F

V

V

W

M

L

D

P

N

E

S

S

I

A

R

R

-

F

-

L

-

V

K

R

A

N

K

R

W

G

L

T

T

D

E

K

S

I

E

R

K

K

D

T

F

L

-

S

-

P

L

I

E

A

A

P

E

Q

I

V

R

V

A

A

D

E

Q

A

K

G

D

S

K

F

H

S

S

V

P

P

K

E

S

Q

T

K

A

A

A

V

V

A

F

A

K

R

D

S

I

V

-

F

-

A

-

V

-

I

-

F

-

S

-

G

-

T

-

Y

-

G

-

L

R

G

I

T

W

M

H

L

M

L

C

W

L

L

I

T

Y

Y

F

F

C

M

I

G

V

L

M

V

G

I

Q

V

Y

Y

G

L

L

L

T

T

F

S

W

W

L

L

P

P

T

T

L

L

V

M

K

R

A

D

S

S

G

G

V

A

V

S

G

M

D

E

F

-

R

Q

I

A

G

A

L

F

I

I

S

G

A

A

I

L

P

F

F

Q

L

F

C

G

A

G

V

V

F

L

A

S

M

A

I

V

L

G

I

V

G

G

R

W

R

A

S

M

D
|
D

R

R

L

Y

R

N

E

P

R
x
H

R

K

W

V

H

I

L

G

I

I

V

F

P

Y

A

L

L

L

L

A

G

G

A

V

I

F

G

A

F

Y

V

A

V

V

S

G

A

Q

I

S

A

L

A

G

D

N

N

I

T

T

V

V

I

L

A

A

I

T

I

L

F

V

L

L

S

-

L

I

A

A

A

G

M

M

G

C

V

V

L

N

T

G

C

A

S

Q

P

S

L

A

F

M

W

P

S

S

L

L

P

A

T

A

A

R

F

F

L

Y

S

P

G

T

T

Q

G

G

A
x
R

A

A

A

T

G

G

I

V

A

S

V

W

I

M

N

L

S

G

V

I

G

G

N
x
R

D41 (= D42) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (≠ N45) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (≠ N83) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (≠ N87) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G90) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R122) mutation to A: Abolishes 4-HBA transport.
E144 (≠ Y142) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ Q188) mutation to A: Decrease in 4-HBA transport and chemotaxis.
D323 (= D312) mutation to N: Abolishes 4-HBA transport and chemotaxis.
H328 (≠ R317) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to R: Decrease in 4-HBA transport and loss of chemotaxis.
R386 (≠ A376) mutation to A: Strong decrease in 4-HBA transport.
R398 (≠ N388) mutation to A: Abolishes 4-HBA transport.

Sites not aligning to the query:

444 H→A: No change in 4-HBA transport and chemotaxis.

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
24% identity, 55% coverage: 42:282/436 of query aligns to 57:283/444 of Q8NLB7

query
sites
Q8NLB7

D
|
D

R

L

V

I

N

V

V

Y

G

G

F

T

A

V

K

Q

L

S

Q

A

M

L

S

A

Q

K

D

E

L

W

G

N

F

L

S

S

E

S

T

A

V

T

F

L

G

G

L

T

G

I

A

G

G

S

I

T

F

A

F

F

L

F

G

G

Y

M

F

A

L

I

F

G

E

A

L

V

P

F

S

I

N

G

L

R

L

L

M

S

N

D

K
x
R

V

V

G

G

A

R

K

K

L

A

W

A

I

V

A

I

R

G

I

S

M

V

I

L

T

I

W

L

G

S

I

V

L

F

S

T

A

M

C

L

F

C

A

A

W

F

V

A

Q

P

T

N

P

P

T

W

Q

V

F

F

Y

G

V

A

L

F

R

R

F

F

L

I

L

A

G

G

L

L

A

G

E

L

A

G

G

G

F

L

Y

V

P

P

G

S

I

V

I

N

L

A

Y

M

L

T

T

S

Y

D

W

L

F

V

P

P

S

R

H

K

R

T

R

M

A

S

K

A

V

W

V

A

A

T

T

V

F

M

M

M

A

S

A

G

I

V

P

P

I

I

A

G

G

G

I

S

F

I

G

A

N

A

P

V

L

L

S

A

G

-

W

-

I

-

M

L

Q

V

A

V

F

V

H

P

G

S

S

S

S

E

G

E

W

W

H

-

G

G

W

W

Q

R

W

F

M

M

F

F

M

L

I

I

E

A

A

L

I

I

P

P

A

-

I

L

L

V

V

V

G

G

I

L

A

P

V

I

V

-

F

-

V

-

M

-

D

-

N

-

S

-

I

-

R

-

K

-

A

A

K

M

W

K

L

V

T

I

E

P

S

S

E

D

K

K

D

-

F

-

L

-

E

-

A

-

E

A

I

I

R

K

A

A

D

D

Q

H

K

-

D

D

K

I

H

R

S

E

P

G

K

H

S

D

T

E

A

P

A

A

V

G

F

F

K

K

D

D

-

L

-

V

-

D

-

R

-

Y

-

R

-

W

I

I

R

S

I

I

W

W

H

-

M

F

C

A

L

L

I

A

Y

T

F

F

C

V

I

T

V

L

M

L

G

A

Q

W

Y

Y

G

G

L

L

T

G

F

T

W

W

L

L

P

P

T

R

L

L

V

M

K

E

A

T

S

A

G

G

D57 (= D42) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (≠ K88) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
23% identity, 81% coverage: 71:424/436 of query aligns to 90:455/493 of Q03567

query
sites
Q03567

F

F

L

Y

G

G

Y

Y

F

I

L

G

F

S

E

Q

L

I

P

I

S

G

N

G

L

H

L

L

M

A

N

S

K

R

V

Y

G

G

A

G

K

K

L

R

W

-

I

V

A

V

R

F

I

V

M

T

I

I

T

L

W

G

G

S

I

A

L

L

S

L

A

T

C

L

F

L

A

N

W

P

V

V

Q

A

T

A

P

R

T

T

Q

S

F

E

Y

Y

-

A

-

L

-

A

V

I

L

L

R

R

F

A

L

A

L

I

G

G

L

F

A

L

E

Q

A

G

G

A

F

T

Y

F

P

P

G

A

I

M

I

H

L

T

Y

M

L

W

T

S

Y

V

W

W

F

G

P

P

S

P

H

L

R

E

R

L

A

S

K

V

V

L

V

T

A

G

T

V

F

T

M

Y

A

A

A

G

I

A

P

Q

I

I

A

G

G

N

I

V

F

I

G

V

N

L

P

P

L

L

S

S

G

G

W

F

I

L

M

C

Q

E

A

-

F

-

H

Y

G

G

S

F

S

D

G

G

W

-

H

-

G

G

W

W

Q

P

W

S

M

I

F

F

M

Y

I

I

E

I

A

G

I

V

P

F

A

G

I

V

L

L

V

W

G

T

I

A

A

V

V

W

F

Y

V

V

M

S

D

S

N

D

S

K

I

P

R

A

K

T

A

H

K

P

W

R

L

I

T

T

E

P

S

E

E

E

K

K

D

Q

F

Y

L

I

E

V

A

T

E

A

I

V

R

E

A

A

D

S

Q

M

-

G

K

K

D

D

K

T

H

G

S

K

P

V

K

P

S

S

T

T

A

P

-

W

-

I

A

K

V

I

F

L

K

T

D

S

I

P

R

A

I

V

W

W

H

A

M

C

C

W

L

A

I

G

Y

H

F

F

C

A

I

G

V

D

M

W

G

G

Q

A

Y

Y

G

T

L

M

T

L

F

V

W

S

L

L

P

P

T

S

L

F

V

L

K

K

A

D

S

V

G

L

V

G

V

L

G

N

D

L

F

S

R

S

I

L

G

G

L

A

I

V

S

A

A

S

I

I

P

P

F

Y

L

I

C

A

A

Y

V

F

F

L

A

A

M

I

I

N

L

A

I

G

G

G

R

V

R

L

S

A

D

D

R

T

L

L

R

R

E

S

-

K

-

G

-

I

-

L

-

S

-

T

-

L

-

N

-

T

R

R

W

A

H

A

L

M

I

L

V

V

P

-

A

A

L

L

L

I

G

G

A

Q

I

G

G

I

F

F

V

L

V

V

S

A

A

S

-

G

-

Y

I

C

A

G

A

C

D

G

N

Q

T

D

V

V

I

L

A

V

I

I

F

F

L

I

S

T

L

C

A

G

-

M

-

A

-

I

-

S

-

G

-

L

-

Q

-

Y

-

A

-

G

-

F

A

V

M

V

G

N

V

Y

L

L

T

E

C

I

S

A

P

P

L

P

F

F

W

-

S

-

L

-

P

-

T

S

A

G

F

T

L

V

S

M

G

G

T

T

G

G

A

-

G

-

I

-

A

-

V

-

I

-

N

N

S

T

V

I

G

S

N

A

L

L

A

A

G

G

F

I

V

I

S

S

P

P

F

A

L

V

V

S

G

S

W

Y

L

L

K

T

D

P

T

N

T

G

H

T

N

Q

N

E

E

E

T

W

G

Q

M

M

-

V

-

L

F

W

M

L

L

T

A

A

G

G

M

I

L

L

V

T

V

I

G

G

A

A

I

L

Sites not aligning to the query:

69 modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 83% coverage: 61:424/436 of query aligns to 122:491/582 of Q9JI12

query
sites
Q9JI12

E

E

T

T

V

V

F

-

G

G

L

M

G

I

A
x
H

G

G

I

S

F

F

L

W

G

G

Y

Y

F

I

L

I

F

T

E

Q

L

I

P

P

S

G

N

G

L

Y

L

I

M

A

N

S

K

R

V

L

G

A

A

A

-

N

K

R

L

V

W

F

I

G

A

A

R

A

I

I

M

L

I

L

T

T

-

S

-

T

W

L

G

N

I

M

L

L

S

I

A

P

C

S

F

A

A

A

W

R

V

V

Q

H

T

Y

P

G

T

C

Q

V

F

I

Y

F

V

V

L

-

R
|
R

F

I

L

L

L

Q

G

G

L

L

A

V

E
|
E

A

G

G

V

F

T

Y

Y

P

P

G

A

I

C

I

H

L

G

Y

I

L

W

T

S

Y

K

W

W

F

A

P

P

S

P

H

L

R

E

R

R

A

S

K

R

V

L

V

A

A

T

T

T

F

S

M

F

A

C

A

G

I

S

P

Y

I

A

A

G

G

A

I

V

F

I

G

A

N

M

P

P

L

L

S

A

G

G

W

I

I

L

M

V

Q

Q

A

-

F

-

H

-

G

-

S

-

G

-

W

Y

H

T

G

G

W

W

Q

S

W

S

M

V

F

F

M

Y

I

V

E

Y

A

G

I

S

P

F

A

G

I

M

L

V

V

W

G

Y

I

M

A

F

V

W

V

L

F

L

V

V

M

S

D

Y

N

E

S

S

I

P

R

A

K

K

A

H

K

P

W

T

L

I

T

T

E

D

S

E

E

E

K

R

D

R

F

Y

L

I

E

E

A

E

E

S

I

I

-

G

-

E

-

S

-

A

-

N

-

L

R

G

A

A

D

M

Q

E

K

K

D

F

K

K

H

T

S

P

P

W

K

R

S

K

T

-

A

-

V

F

F

F

K

T

D

S

I

M

R

P

I

V

W

Y

H

A

M

I

C

I

L

V

I

A

Y

N

F

F

C

C

I
x
R

V

S

M

W

G

T

Q

F

Y

Y

G

L

L

L

T

L

F

I

W

S

L

Q

P

P

T

A

L

Y

V

F

K

E

A

E

S

V

G

F

V

G

V

F

G

E

D

I

F

S

R

K

I

V

G

G

L

M

I

L

S

S

A

A

I

V

P

P

F

H

L

L

C

V

A

M

V

T

F

I

A

I

M

V

I

P

L

I

I

G

G

G

R

Q

R

I

S

A

D

D

R

F

L

L

R

R

E

S

R

K

R

Q

-

I

-

L

W

S

H

T

L

T

I

T

V

V

P

R

A

K

L

I

L

M

G

N

A

C