SitesBLAST

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
29% identity, 41% coverage: 20:231/519 of query aligns to 2:212/240 of 4ymuJ

query
sites
4ymuJ

L

I

T

F

L

V

S

N

G

D

I

V

C

Y

K

K

A

N

F
|
F

G

G

G

S

V

L

T

E

A
x
V

L

L

N

K

E

G

V

V

A

T

L

L

R

K

L

V

Y

N

P

K

G

G

E

E

V

V

H

V

T

V

L

I

M

I

G

G

Q

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

V

C

L

I

T

N

G

L

V

L

Y

E

T

E

P

P

D

T

S

K

G

G

R

E

I

V

L

F

L

I

H

D

G

G

Q

V

P

K

V

I

Q

N

P

N

-

G

R

K

S

V

T

N

L

I

E

N

A

K

Q

V

S

R

L

Q

G

K

I

V

S

G

T

M

V

V

Y

F

Q

Q

E

H

V

F

N

N

L

L

C

F

P

P

N

H

L

L

S

T

V

A

A

I

E

E

N

N

I

I

F

T

I

L

G

A

R

-

Y

-

P

P

K

V

K

K

Y

V

G

K

A

K

I

M

D

N

W

K

K

K

S

-

M

-

Q

-

Q

E

A

A

Q

E

L

L

L

A

H

V

E

D

L

L

H

L

V

A

R

K

I

V

D

G

V

L

A

L

A

D

P

K

L

K

S

D

R

Q

Y

Y

P

P

L

I

A

K

I

L

-

S

-

G

-

Q

Q

K

Q

Q

M

R

V

L

A

A

I

I

S

A

R

R

A

A

L

L

S

A

I

M

S

Q

A

P

K

E

V

V

L

M

I

L

L

F

D

D

E
|
E

P

P

T

T

S

S

S

A

L

L

D

D

D

P

A

E

E

M

V

V

K

K

L

E

L

V

F

L

E

N

V

V

L

M

R

K

K

Q

L

L

R

A

A

N

Q

E

G

G

M

M

A

T

I

M

L

V

F

V

V

V

T

T

H
|
H

F

E

L

M

E

G

Q

F

T

A

Y

R

E

E

I

V

S

G

D

D

R

R

I

V

T

I

V

F

L

M

R

D

N

D

G

G

binding adenosine-5'-triphosphate: F11 (= F29), V16 (≠ A34), S36 (≠ N54), G37 (= G55), S38 (≠ A56), G39 (= G57), K40 (= K58), S41 (= S59), T42 (= T60), E162 (= E181), H194 (= H213)
binding magnesium ion: S41 (= S59), E162 (= E181)

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
33% identity, 42% coverage: 18:234/519 of query aligns to 1:212/358 of 8y5iA

query
sites
8y5iA

P

P

A

L

L

V

T

Q

L

L

S

A

G

G

I

I

C

R

K

K

A

C

F
|
F

G

D

G

G

V
x
K

T

E

A

V

L

I

N

P

E

Q

V

L

A

D

L

L

R

T

L

I

Y

N

P

N

G

G

E

E

V

F

H

L

T

T

L

L

M

L

G

G

Q

P

N

S

G
|
G

A

C

G

G

K
|
K

S
x
T

T
|
T

L

V

I

L

K

R

V

L

L

I

T

A

G

G

V

L

Y

E

T

T

P

V

D

D

S

S

G

G

R

R

I

I

L

M

L

L

H

D

G

N

Q

E

P

D

V

I

Q

-

P

-

R

-

S

T

T

H

L

V

E

P

A

A

Q

E

S

N

L

R

G

Y

I

V

S

N

T

T

V

V

Y

F

Q

Q

E

S

V

Y

N

A

L

L

C

F

P

P

N

H

L

M

S

T

V

V

A

F

E

E

N

N

I

V

F

A

I

F

G

G

R

L

Y

R

P

M

K

Q

K

K

Y

T

G

P

A

A

I

A

D

E

W

I

K

T

S

P

M

R

Q

V

Q

M

Q

E

A

A

Q

L

Q

R

L

M

L

-

H

-

E

-

L

-

H

-

V

V

R

Q

I

L

D

E

V

T

A

F

A

A

P

Q

L
x
R

S

K

R

P

Y

H

P
x
Q

L

L

A
x
S

-

G

I

Q

Q

Q

M

R

V

V

A

A

I

I

S

A

R

R

A

A

L

V

S

V

I

N

S

K

A

P

K

R

V

L

L

L

I

L

L

L

D

D

E

Q

P

S

T

L

S

S

S

A

L

L

D

D

D

Y

A

K

E

L

V

R

K

K

L

Q

L

M

F

Q

E

N

V

E

L

L

R

K

K

A

L

L

-

Q

R

R

A

K

Q

L

G

G

M

I

A

T

I

F

L

V

F

F

V

V

T

T

H

H

F

D

L

Q

E

E

Q

E

T

A

Y

L

E

T

I

M

S

S

D

D

R

R

I

I

T

V

V

V

L

M

R

R

N

D

G

G

Q

R

L

I

E

E

binding adenosine-5'-triphosphate: F12 (= F29), K15 (≠ V32), G38 (= G55), K41 (= K58), T42 (≠ S59), T43 (= T60), R128 (≠ L153), Q132 (≠ P157), S134 (≠ A159)

5x40A Structure of a cbio dimer bound with amppcp (see paper)
31% identity, 42% coverage: 18:236/519 of query aligns to 3:221/280 of 5x40A

query
sites
5x40A

P

P

A

I

L

L

T

A

L

A

S

E

G

A

I

L

C

T

K

Y

A

A

F
|
F

-

P

G

G

V
|
V

T

K

A
|
A

L

L

N

D

E

D

V

L

A

S

L

L

R

A

L

V

Y

P

P

K

G

G

E

E

V

S

H

L

T

A

L

I

M

L

G

G

Q

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

L

V

H

L

L

T

N

G

G

V

T

Y

L

T

R

P

P

D

Q

S

S

G

G

R

R

I

V

L

L

H

G

G

G

Q

T

P

A

V

T

-

G

Q

H

P

S

R

R

S

K

T

D

L

L

E

T

A

G

-

W

-

R

-

V

-

G

-

L

-

V

-

L

-
x
Q

-

D

-

A

-

D

Q

D

S

Q

L

L

G

F

I

A

S

T

T

T

V

V

Y

F

Q

E

E

D

V

V

N

S

L

F

C

G

P

P

-

L

N

N

L

L

S

G

V

L

A

S

E

E

N

A

I

E

F

A

I

R

G

A

R

R

Y

V

P

E

K

E

K

A

Y

L

G

A

A

A

I

L

D

S

W

I

K

S

S

D

M

L

Q

R

Q

D

Q

R

A

P

Q

T

Q
x
H

L
x
M

L
|
L

H
x
S

E

G

L

-

H

-

V

-

R

-

I

-

D

-

V

-

A

-

P

-

L

-

S

-

R

-

Y

-

P

-

L

-

A
x
G

I
x
Q

Q

K

Q

R

M

R

V

V

A

A

I

I

S

A

R

G

A

A

L

V

S

A

I

M

S

R

A

P

K

E

V

V

L

L

I

L

L

L

D

D

E
x
Q

P

P

T

T

S

A

S

G

L

L

D

D

D

L

A

A

E

G

V

T

K

E

L

Q

L

L

F

L

E

T

V

L

L

L

R

R

K

G

L

L

R

R

A

A

Q

A

G

G

M

M

A

T

I

L

L

V

F

F

V

S

T

T

H
|
H

F

D

L

V

E

E

Q

L

T

A

Y

A

E

A

I

L

S

A

D

D

R

R

I

V

T

A

V

L

L

F

R

R

N

T

G

G

Q

R

L

V

E

L

G

A

E

E

binding phosphomethylphosphonic acid adenylate ester: F14 (= F29), V18 (= V32), A20 (= A34), N40 (= N54), G41 (= G55), G43 (= G57), K44 (= K58), S45 (= S59), T46 (= T60), Q88 (vs. gap), H139 (≠ Q138), M140 (≠ L139), L141 (= L140), S142 (≠ H141), G144 (≠ A159), Q145 (≠ I160), Q166 (≠ E181), H198 (= H213)
binding magnesium ion: S45 (= S59), Q88 (vs. gap)

3d31A Modbc from methanosarcina acetivorans (see paper)
34% identity, 41% coverage: 21:233/519 of query aligns to 6:206/348 of 3d31A

query
sites
3d31A

T

S

L

L

S

S

G

R

I

K

C

W

K

K

A

N

F

F

G

-

V

-

T

-

A

S

L

L

N

D

E

N

V

L

A

S

L

L

R

K

L

V

Y

E

P

S

G

G

E

E

V

Y

H

F

T

V

L

I

M

L

G

G

Q

P

N

T

G

G

A

A

G

G

K

K

S

T

T

L

L

F

I

L

K

E

V

L

L

I

T

A

G

G

V

F

Y

H

T

V

P

P

D

D

S

S

G

G

R

R

I

I

L

L

H

D

G

G

Q

K

P

D

V

V

Q

-

P

-

R

-

S

T

T

D

L

L

E

S

A

P

Q

E

S

K

L

H

G

D

I

I

S

A

T

F

V

V

Y

Y

Q

Q

E

N

V

Y

N

S

L

L

C

F

P

P

N

H

L

M

S

N

V

V

A

K

E

K

N

N

I

L

F

E

I

F

G

G

R

M

Y

R

P

M

K

K

Y

I

G

K

A

-

I

-

D

D

W

P

K

K

S

R

M

V

Q

L

Q

D

Q

T

A

A

Q

R

Q

D

L

L

L

K

H

I

E

E

L

-

H

H

V

L

R

-

I

-

D

-

V

-

A

-

P

-

L

L

S

D

R

R

Y

N

P

P

L

L

A

T

I

L

-

S

-

G

-

E

Q

Q

M

R

V

V

A

A

I

L

S

A

R

R

A

A

L

L

S

V

I

T

S

N

A

P

K

K

V

I

L

L

I

L

L

L

D

D

E

E

P

P

T

L

S

S

S

A

L

L

D

D

D

P

A

R

E

T

V

Q

K

E

L

N

L

A

F

R

E

E

V

M

L

L

R

S

K

V

L

L

R

H

A

K

Q

K

G

N

-

K

M

L

A

T

I

V

L

L

F

H

V

I

T

T

H

H

F

D

L

Q

E

T

Q

E

T

A

Y

R

E

I

I

M

S

A

D

D

R

R

I

I

T

A

V

V

L

V

R

M

N

D

G

G

Q

K

L

L

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

3c4jA Abc protein artp in complex with atp-gamma-s
30% identity, 40% coverage: 27:233/519 of query aligns to 11:216/242 of 3c4jA

query
sites
3c4jA

K

K

A

S

F
|
F

G

G

G

S

V

L

T

E

A
x
V

L

L

N

K

E

G

V

I

A

N

L

V

R

H

L

I

Y

R

P

E

G

G

E

E

V

V

H

V

T

V

L

V

M

I

G

G

Q

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

V

C

L

L

T

N

G

L

V

L

Y

E

T

D

P

F

D

D

S

E

G

G

R

E

I

I

L

I

H

D

G

G

Q

I

P

N

V

L

Q

K

P

A

R

K

S

D

T

T

-

N

L

L

E

N

A

K

Q

V

S

R

L

E

G

E

I

V

S

G

T

M

V

V

Y

F

Q

Q

E

R

V

F

N

N

L

L

C

F

P

P

N

H

L

M

S

T

V

V

A

L

E

N

N

N

I

I

F

T

I

L

G

A

R

-

Y

-

P

P

K

M

K

K

Y

V

G

R

A

K

I

W

D

P

W

R

K

E

S

K

M

A

Q

E

Q

A

Q

K

A

A

Q

M

Q

E

L

L

H

D

E

K

L

V

H

G

V

L

R

K

I

D

D

K

V

A

A

H

A

A

P

-

L

-

S

-

R

-

Y

Y

P

P

L

D

A

S

I

L

Q

S

-

G

-

Q

-

A

Q

Q

M

R

V

V

A

A

I

I

S

A

R

R

A

A

L

L

S

A

I

M

S

E

A

P

K

K

V

I

L

M

I

L

L

F

D

D

E

E

P

P

T

T

S

S

S

A

L

L

D

D

D

P

A

E

E

M

V

V

K

G

L

E

L

V

F

L

E

S

V

V

L

M

R

K

K

Q

L

L

R

A

A

N

Q

E

G

G

M

M

A

T

I

M

L

V

F

V

V

V

T

T

H

H

F

E

L

M

E

G

Q

F

T

A

Y

R

E

E

I

V

S

G

D

D

R

R

I

V

T

L

V

F

L

M

R

D

N

G

G

G

Q

Y

L

I

binding phosphothiophosphoric acid-adenylate ester: F13 (= F29), V18 (≠ A34), S38 (≠ N54), G39 (= G55), S40 (≠ A56), G41 (= G57), K42 (= K58), S43 (= S59), T44 (= T60)

3c41J Abc protein artp in complex with amp-pnp/mg2+
30% identity, 40% coverage: 27:233/519 of query aligns to 11:216/242 of 3c41J

query
sites
3c41J

K

K

A

S

F
|
F

G

G

G

S

V

L

T

E

A
x
V

L

L

N

K

E

G

V

I

A

N

L

V

R

H

L

I

Y

R

P

E

G

G

E

E

V

V

H

V

T

V

L

V

M

I

G

G

Q

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

V

C

L

L

T

N

G

L

V

L

Y

E

T

D

P

F

D

D

S

E

G

G

R

E

I

I

L

I

H

D

G

G

Q

I

P

N

V

L

Q

K

P

A

R

K

S

D

T

T

-

N

L

L

E

N

A

K

Q

V

S

R

L

E

G

E

I

V

S

G

T

M

V

V

Y

F

Q

Q

E

R

V

F

N

N

L

L

C

F

P

P

N

H

L

M

S

T

V

V

A

L

E

N

N

N

I

I

F

T

I

L

G

A

R

-

Y

-

P

P

K

M

K

K

Y

V

G

R

A

K

I

W

D

P

W

R

K

E

S

K

M

A

Q

E

Q

A

Q

K

A

A

Q

M

Q

E

L

L

H

D

E

K

L

V

H

G

V

L

R

K

I

D

D

K

V

A

A

H

A

A

P

-

L

-

S

-

R

-

Y

Y

P

P

L

D

A

S

I

L

Q

S

-

G

-

Q

-

A

Q

Q

M

R

V

V

A

A

I

I

S

A

R

R

A

A

L

L

S

A

I

M

S

E

A

P

K

K

V

I

L

M

I

L

L

F

D
|
D

E

E

P

P

T

T

S

S

S

A

L

L

D

D

D

P

A

E

E

M

V

V

K

G

L

E

L

V

F

L

E

S

V

V

L

M

R

K

K

Q

L

L

R

A

A

N

Q

E

G

G

M

M

A

T

I

M

L

V

F

V

V

V

T

T

H

H

F

E

L

M

E

G

Q

F

T

A

Y

R

E

E

I

V

S

G

D

D

R

R

I

V

T

L

V

F

L

M

R

D

N

G

G

G

Q

Y

L

I

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F29), V18 (≠ A34), S38 (≠ N54), G39 (= G55), G41 (= G57), K42 (= K58), S43 (= S59), T44 (= T60)
binding magnesium ion: S43 (= S59), D163 (= D180)

2olkA Abc protein artp in complex with adp-beta-s
30% identity, 40% coverage: 27:233/519 of query aligns to 11:216/242 of 2olkA

query
sites
2olkA

K

K

A

S

F
|
F

G

G

G

S

V

L

T

E

A
x
V

L

L

N

K

E

G

V

I

A

N

L

V

R

H

L

I

Y

R

P

E

G

G

E

E

V

V

H

V

T

V

L

V

M

I

G

G

Q

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

V

C

L

L

T

N

G

L

V

L

Y

E

T

D

P

F

D

D

S

E

G

G

R

E

I

I

L

I

H

D

G

G

Q

I

P

N

V

L

Q

K

P

A

R

K

S

D

T

T

-

N

L

L

E

N

A

K

Q

V

S

R

L

E

G

E

I

V

S

G

T

M

V

V

Y

F

Q

Q

E

R

V

F

N

N

L

L

C

F

P

P

N

H

L

M

S

T

V

V

A

L

E

N

N

N

I

I

F

T

I

L

G

A

R

-

Y

-

P

P

K

M

K

K

Y

V

G

R

A

K

I

W

D

P

W

R

K

E

S

K

M

A

Q

E

Q

A

Q

K

A

A

Q

M

Q

E

L

L

H

D

E

K

L

V

H

G

V

L

R

K

I

D

D

K

V

A

A

H

A

A

P

-

L

-

S

-

R

-

Y

Y

P

P

L

D

A

S

I

L

Q

S

-

G

-

Q

-

A

Q

Q

M

R

V

V

A

A

I

I

S

A

R

R

A

A

L

L

S

A

I

M

S

E

A

P

K

K

V

I

L

M

I

L

L

F

D

D

E

E

P

P

T

T

S

S

S

A

L

L

D

D

D

P

A

E

E

M

V

V

K

G

L

E

L

V

F

L

E

S

V

V

L

M

R

K

K

Q

L

L

R

A

A

N

Q

E

G

G

M

M

A

T

I

M

L

V

F

V

V

V

T

T

H

H

F

E

L

M

E

G

Q

F

T

A

Y

R

E

E

I

V

S

G

D

D

R

R

I

V

T

L

V

F

L

M

R

D

N

G

G

G

Q

Y

L

I

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F29), V18 (≠ A34), S38 (≠ N54), G39 (= G55), S40 (≠ A56), G41 (= G57), K42 (= K58), S43 (= S59), T44 (= T60)

2oljA Abc protein artp in complex with adp/mg2+
30% identity, 40% coverage: 27:233/519 of query aligns to 11:216/242 of 2oljA

query
sites
2oljA

K

K

A

S

F
|
F

G

G

G

S

V

L

T

E

A
x
V

L

L

N

K

E

G

V

I

A

N

L

V

R

H

L

I

Y

R

P

E

G

G

E

E

V

V

H

V

T

V

L

V

M

I

G

G

Q

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

V

C

L

L

T

N

G

L

V

L

Y

E

T

D

P

F

D

D

S

E

G

G

R

E

I

I

L

I

H

D

G

G

Q

I

P

N

V

L

Q

K

P

A

R

K

S

D

T

T

-

N

L

L

E

N

A

K

Q

V

S

R

L

E

G

E

I

V

S

G

T

M

V

V

Y

F

Q

Q

E

R

V

F

N

N

L

L

C

F

P

P

N

H

L

M

S

T

V

V

A

L

E

N

N

N

I

I

F

T

I

L

G

A

R

-

Y

-

P

P

K

M

K

K

Y

V

G

R

A

K

I

W

D

P

W

R

K

E

S

K

M

A

Q

E

Q

A

Q

K

A

A

Q

M

Q

E

L

L

H

D

E

K

L

V

H

G

V

L

R

K

I

D

D

K

V

A

A

H

A

A

P

-

L

-

S

-

R

-

Y

Y

P

P

L

D

A

S

I

L

Q

S

-

G

-

Q

-

A

Q

Q

M

R

V

V

A

A

I

I

S

A

R

R

A

A

L

L

S

A

I

M

S

E

A

P

K

K

V

I

L

M

I

L

L

F

D

D

E

E

P

P

T

T

S

S

S

A

L

L

D

D

D

P

A

E

E

M

V

V

K

G

L

E

L

V

F

L

E

S

V

V

L

M

R

K

K

Q

L

L

R

A

A

N

Q

E

G

G

M

M

A

T

I

M

L

V

F

V

V

V

T

T

H

H

F

E

L

M

E

G

Q

F

T

A

Y

R

E

E

I

V

S

G

D

D

R

R

I

V

T

L

V

F

L

M

R

D

N

G

G

G

Q

Y

L

I

binding adenosine-5'-diphosphate: F13 (= F29), V18 (≠ A34), S38 (≠ N54), G39 (= G55), S40 (≠ A56), G41 (= G57), K42 (= K58), S43 (= S59), T44 (= T60)

Q5SSE9 ATP-binding cassette sub-family A member 13; EC 7.6.2.- from Mus musculus (Mouse) (see paper)
30% identity, 43% coverage: 10:233/519 of query aligns to 3801:4020/5034 of Q5SSE9

query
sites
Q5SSE9

G

G

E

E

A

M

Q

E

G

G

S

-

G

G

A

N

P

P

A

G

L

V

T

A

L

L

S

I

G

S

I

V

C

T

K

K

A

E

F

Y

-

E

G

D

G

H

V

K

T

V

A

A

L

V

N

Q

E

E

V

L

A

T

L

L

R

T

L

F

Y

H

P

R

G

D

E

Q

V

I

H

T

T

A

L

L

M

L

G

G

Q

T

N

N

G

G

A

A

G

G

K
|
K

S

T

T

T

L

I

I

I

K

S

V

M

L

L

T

M

G

G

V

L

Y

F

T

P

P

P

D

T

S

S

G

G

R

T

I

I

L

T

L

I

H

N

G

G

Q

K

P

N

V

L

Q

Q

P

T

R

-

S

-

T

-

L

-

E

-

A

-

Q

-

S

-

L

-

G

D

I

L

S

S

T

K

V

V

Y

R

Q

E

E

E

V

L

N

G

L

V

C

C

P

P

-

Q

-

D

-

V

-

L

-

D

N

N

L

L

S

T

V

V

A

R

E

E

N

H

I

L

F

M

I

L

G

F

R

A

Y

S

P

I

K

K

K

-

Y

-

G

-

A

A

I

P

D

W

W

W

-

T

K

K

S

E

M

L

Q

Q

A

V

Q

N

Q

K

L

T

L

L

H

D

E

E

L

V

H

E

V

L

R

T

I

Q

D

H

V

Q

A

H

A

K

P

P

L

A

S

G

R

V

Y

L

P

S

L

G

A

G

I

M

Q

K

Q

R

M

K

V

L

A

S

I

I

S

G

R

I

A

A

L

F

S

M

I

G

S

M

A

S

K

K

V

T

L

V

I

V

L

L

D

D

E

E

P

P

T

S

S

S

S

G

L

V

D

D

D

P

A

C

E

S

V

R

K

R

L

S

L

L

F

W

E

D

V

I

L

L

R

L

K

K

L

Y

R

R

A

-

Q

E

G

G

M

R

A

T

I

I

L

I

F

F

V

T

T
|
T

H

H

F

H

L

L

E

D

Q

E

T

A

Y

E

E

M

I

L

S

S

D

D

R

H

I

V

T

A

V

V

L

L

R

Q

N

Q

G

G

Q

R

L

L

K3849 (= K58) mutation to M: Does not affect intracellular vesicle localization. Affects cholesterol internalization.
T3999 (= T212) mutation to A: Does not affect protein expression. Affects intracellular vesicles localization. Impairs intracellular cholesterol accumulation in the vesicle.

Sites not aligning to the query:

3577 H→P: Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.
4735 K→M: Does not affect intracellular vesicle localization.Affects cholesterol internalization.
4818 R→C: Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.

8k1pB Mycobacterial efflux pump, adp+vanadate bound state
31% identity, 41% coverage: 20:232/519 of query aligns to 4:206/213 of 8k1pB

query
sites
8k1pB

L

I

T

E

L

I

S

R

G

G

I

L

C

T

K

K

A

H

F
|
F

G

G

G

S

V

V

T

R

A

A

L

L

N

D

E

G

V

L

A

D

L

L

R

T

L

V

Y

R

P

E

G

G

E

E

V

V

H

H

T

G

L

F

M

L

G

G

Q

P

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

T

I

L

K

R

V

I

L

L

T

L

G

G

V

L

Y

V

T

K

P

A

D

D

S

G

G

G

R

S

I

V

-

R

L

L

H

G

G

G

Q

D

P

P

V

W

Q

T

P

D

R

A

S

V

T

D

L

L

E

H

A

R

Q

H

S

-

L

-

G

-

I

I

S

A

T

Y

V

V

Y

P

Q

G

E

D

V

V

N

T

L

L

C

W

P

P

N

S

L

L

S

T

V

G

A

G

E

E

N

T

I

I

F

D

I

L

G

-

R

-

Y

L

P

A

K

R

K

M

Y

R

G

G

A

G

I

I

D

D

W

-

K

-

S

-

M

-

Q

N

Q

A

Q

R

A

R

Q

A

Q

E

L

L

L

I

H

E

E

R

L

F

H

G

V

-

R

-

I

L

D

D

V

P

A

T

A

K

P

K

L

A

S

R

R
x
T

Y

Y

P
x
S

L

K

A

G

I

N

Q

R

Q

Q

M

K

V

V

A

S

I

L

S

I

R

S

A

A

L

L

S

S

I

S

S

H

A

A

K

T

V

L

L

L

I

L

L

L

D

D

E

E

P

P

T

S

S

S

S

G

L

L

D

D

D

P

A

L

E

M

V

E

K

N

L

V

L

F

F

Q

E

Q

V

C

L

I

R

G

K

E

L

A

R

R

A

Q

Q

R

G

G

M

V

A

T

I

V

L

L

F

L

V

S

T

S

H

H

F

I

L

L

E

A

Q

E

T

T

Y

E

E

A

I

L

S

C

D

E

R

K

I

V

T

T

V

I

L

I

R

R

N

A

G

G

Q

K

binding adenosine-5'-diphosphate: F13 (= F29), N38 (= N54), G39 (= G55), A40 (= A56), G41 (= G57), K42 (= K58), S43 (= S59), T44 (= T60)
binding adenosine-5'-triphosphate: T129 (≠ R155), S131 (≠ P157)

8k1oB Mycobacterial efflux pump, amppnp bound state
31% identity, 41% coverage: 20:232/519 of query aligns to 6:208/215 of 8k1oB

query
sites
8k1oB

L

I

T

E

L

I

S

R

G

G

I

L

C

T

K

K

A

H

F

F

G

G

G

S

V

V

T

R

A

A

L

L

N

D

E

G

V

L

A

D

L

L

R

T

L

V

Y

R

P

E

G

G

E

E

V

V

H

H

T

G

L

F

M

L

G

G

Q

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

T

I

L

K

R

V

I

L

L

T

L

G

G

V

L

Y

V

T

K

P

A

D

D

S

G

G

G

R

S

I

V

-

R

L

L

H

G

G

G

Q

D

P

P

V

W

Q

T

P

D

R

A

S

V

T

D

L

L

E

H

A

R

Q

H

S

-

L

-

G

-

I

I

S

A

T

Y

V

V

Y

P

Q

G

E

D

V

V

N

T

L

L

C

W

P

P

N

S

L

L

S

T

V

G

A

G

E

E

N

T

I

I

F

D

I

L

G

-

R

-

Y

L

P

A

K

R

K

M

Y

R

G

G

A

G

I

I

D

D

W

-

K

-

S

-

M

-

Q

N

Q

A

Q

R

A

R

Q

A

Q

E

L

L

L

I

H

E

E

R

L

F

H

G

V

-

R

-

I

L

D

D

V

P

A

T

A

K

P

K

L

A

S

R

R
x
T

Y

Y

P
x
S

L

K

A

G

I

N

Q

R

Q

Q

M

K

V

V

A

S

I

L

S

I

R

S

A

A

L

L

S

S

I

S

S

H

A

A

K

T

V

L

L

L

I

L

L

L

D

D

E

E

P

P

T

S

S

S

S

G

L

L

D

D

D

P

A

L

E

M

V

E

K

N

L

V

L

F

F

Q

E

Q

V

C

L

I

R

G

K

E

L

A

R

R

A

Q

Q

R

G

G

M

V

A

T

I

V

L

L

F

L

V

S

T

S

H

H

F

I

L

L

E

A

Q

E

T

T

Y

E

E

A

I

L

S

C

D

E

R

K

I

V

T

T

V

I

L

I

R

R

N

A

G

G

Q

K

binding phosphoaminophosphonic acid-adenylate ester: N40 (= N54), G43 (= G57), K44 (= K58), S45 (= S59), T46 (= T60), T131 (≠ R155), S133 (≠ P157)

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
32% identity, 41% coverage: 19:233/519 of query aligns to 3:216/350 of 3fvqB

query
sites
3fvqB

A

A

L

L

T

H

L

I

S

G

G

H

I

L

C

S

K

K

A

S

F
|
F

G
x
Q

G

N

V
x
T

T

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binding adenosine-5'-triphosphate: F13 (= F29), Q14 (≠ G30), T16 (≠ V32), V18 (≠ A34), S38 (≠ N54), G39 (= G55), C40 (≠ A56), G41 (= G57), K42 (= K58), T43 (≠ S59), T44 (= T60), R133 (= R155), E137 (≠ A159), S139 (≠ Q161), G141 (vs. gap), Q142 (vs. gap)
binding calcium ion: T43 (≠ S59), Q86 (= Q101)

Q8R420 Phospholipid-transporting ATPase ABCA3; ATP-binding cassette sub-family A member 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Mus musculus (Mouse) (see paper)
31% identity, 39% coverage: 35:234/519 of query aligns to 549:742/1704 of Q8R420

query
sites
Q8R420

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Sites not aligning to the query:

292 E→V: Knockin new born mice are healthy and survive into adulthood without overt signs of respiratory distress. Knockin mice show a severe lung phenotype that begins with alveolar inflammatory cell infiltration at the early stage of the mouse life followed by aberrant lung remodeling with characteristics of diffuse parenchymal lung disease (DPLD)- and emphysema-like alveolar disruption in older mice.

7mdyC Lolcde nucleotide-bound
33% identity, 42% coverage: 20:236/519 of query aligns to 3:223/226 of 7mdyC

query
sites
7mdyC

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binding adp orthovanadate: Y12 (≠ F29), G42 (= G55), S43 (≠ A56), G44 (= G57), K45 (= K58), S46 (= S59), T47 (= T60), Q91 (= Q101), H138 (≠ V145), E142 (≠ R155), S144 (≠ P157), G145 (≠ L158), G146 (≠ A159), E168 (= E181), N172 (≠ S185), H201 (= H213)
binding magnesium ion: S46 (= S59), Q91 (= Q101)

Q99758 Phospholipid-transporting ATPase ABCA3; ABC-C transporter; ATP-binding cassette sub-family A member 3; ATP-binding cassette transporter 3; ATP-binding cassette 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Homo sapiens (Human) (see 15 papers)
31% identity, 39% coverage: 34:234/519 of query aligns to 548:742/1704 of Q99758

query
sites
Q99758

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N568 (= N54) to D: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; does not affect protein expression; does not affect multivesicular bodies and lamellar bodies location; affects multivesicular bodies and lamellar bodies development; loss of phosphatidylcholine transport; does not affect cholesterol transport; dbSNP:rs121909184
L579 (= L65) to P: in SMDP3; uncertain significance
R605 (≠ A91) to Q: in SMDP3; uncertain significance; dbSNP:rs760006956
S693 (= S184) mutation to L: Does not affect protein oligomerization.

Sites not aligning to the query:

43 R → L: in SMDP3; uncertain significance
53 N→Q: Does not affect N-glycosylation. Does not affect protein expression. Does not affect lamellar body membrane location.
101 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; dbSNP:rs121909182
124 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-140. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
140 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N → H: in dbSNP:rs45447801; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-124. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
173:174 LK→AA: Loss of proteolytic processing.
174:175 Cleavage; by CTSL
215 Q → K: in SMDP3; loss of lamellar bodies membrane location; loss of proteolytic cleavage; increases cellular free cholesterol and phosphatidylcholine transport; loss of vesicles formation; increases free cholesterol induced cell death; loss of protein oligomerization; dbSNP:rs879159551
280 R → C: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs201299260
288 R → K: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs117603931
290 L → M: in a breast cancer sample; somatic mutation
292 E → V: in SMDP3; uncertain significance; does not affect lamellar bodies membrane location; does not affect proteolytic cleavage; affects lamellar bodies formation; does not affect cholesterol and phosphatidylcholine transport; decreases vesicles formation; does not affect free cholesterol induced cell death; dbSNP:rs149989682
766 P → S: in dbSNP:rs45592239
801 E → D: in a breast cancer sample; somatic mutation
945 N→Q: Does not affect lamellar body membrane location. Does not affect protein expression. Does not affect proteolytic processing.
982 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs1402761450
1069 H → Q: in a breast cancer sample; somatic mutation
1076 N → K: in SMDP3; uncertain significance; dbSNP:rs2093663770
1221 G → S: in SMDP3; does not affect intracellular vesicle membrane location; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; G→A: Decreases ATP hydrolysis activity of 15% compared to the wild-type.; G→T: Decreases ATP hydrolysis activity of 36% compared to the wild-type.; G→V: Decreases ATP hydrolysis activity of 18% compared to the wild-type.
1302 G → E: in SMDP3; uncertain significance; dbSNP:rs2093657978
1388 K → N: in SMDP3; decreases phosphatidylcholine transport; increases protein abundance; does not affect folding in the endoplasmic reticulum; decreases proteolytic processing; affects lamellar bodies development; reduces free cholesterol transport
1553 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs121909183
1580 L → P: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; affects the intracellular vesicles development; decreases phosphatidylcholine transport; L→A: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→F: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→V: Decreases ATP hydrolysis activity of 56% compared to the wild-type.
1591 Q → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs28936691

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
27% identity, 44% coverage: 20:245/519 of query aligns to 5:226/285 of 4yerA

query
sites
4yerA

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L

M

F

W

E

E

V

Y

L

I

R

S

K

K

L

M

R

K

A

K

Q

E

-

H

G

N

M

M

A

T

I

I

L

F

F

L

V

T

T

T

H

H

F

Y

L

M

E

D

Q

E

T

A

Y

E

E

Q

I

L

S

A

D

D

R

R

I

V

T

A

V

I

L

I

R

D

N

H

G

G

Q

K

L

I

E

I

G

A

E

L

Y

G

L

T

A

P

S

T

Q

E

L

L

S

K

R

R

L

M

binding adenosine-5'-diphosphate: F14 (= F29), F17 (≠ V32), N39 (= N54), G40 (= G55), G42 (= G57), K43 (= K58), T44 (≠ S59), T45 (= T60), T135 (≠ R155), F136 (≠ Y156), S137 (≠ P157)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 41% coverage: 20:233/519 of query aligns to 2:219/343 of P30750

query
sites
P30750

L

I

T

K

L

L

S

S

G

N

I

I

C

T

K

K

A

V

F

F

G

H

-

Q

-

G

-

T

-

R

G

T

V

I

T

Q

A

A

L

L

N

N

E

N

V

V

A

S

L

L

R

H

L

V

Y

P

P

A

G

G

E

Q

V

I

H

Y

T

G

L

V

M

I

G

G

Q

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

V

C

L

V

T

N

G

L

V

L

Y

E

T

R

P

P

D

T

S

E

G

G

R

S

I

V

L

L

L

V

H

D

G

G

Q

Q

P

E

V

L

Q

T

-

T

-

L

P

S

R

E

S

S

T

E

L

L

E

T

A

K

Q

A

S

R

L

R

G

Q

I

I

S

G

T

M

V

I

Y

F

Q

Q

E

H

V

F

N

N

L

L

C

L

P

S

N

S

L

R

S

T

V

V

A

F

E

G

N

N

I

V

F

A

I

L

G

-

R

-

Y

-

P

P

K

L

K

E

Y

L

G

D

A

N

I

T

D

P

W

K

K

D

S

E

M

V

Q

K

Q

R

A

V

Q

T

Q

E

L

L

H

S

E

-

L

-

H

-

V

-

R

L

I

V

D

G

V

L

A

G

A

D

P

K

L

H

S

D

R

S

Y

Y

P

P

L

S

A

N

I

L

-

S

-

G

-

Q

Q

K

Q

Q

M

R

V

V

A

A

I

I

S

A

R

R

A

A

L

L

S

A

I

S

S

N

A

P

K

K

V

V

L

L

I

L

L

C

D

D

E
|
E

P

A

T

T

S

S

S

A

L

L

D

D

D

P

A

A

E

T

V

T

K

R

L

S

L

I

F

L

E

E

V

L

L

L

R

K

K

D

L

I

-

N

R

R

A

R

Q

L

G

G

M

L

A

T

I

I

L

L

F

L

V

I

T

T

H

H

F

E

L

M

E

D

Q

V

T

V

Y

K

E

R

I

I

S

C

D

D

R

C

I

V

T

A

V

V

L

I

R

S

N

N

G

G

Q

E

L

L

40:46 (vs. 54:60, 86% identical) binding ATP
E166 (= E181) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

Query Sequence

>WP_061941304.1 NCBI__GCF_001584185.1:WP_061941304.1
MSKTEIGTNGEAQGSGAPALTLSGICKAFGGVTALNEVALRLYPGEVHTLMGQNGAGKST
LIKVLTGVYTPDSGRILLHGQPVQPRSTLEAQSLGISTVYQEVNLCPNLSVAENIFIGRY
PKKYGAIDWKSMQQQAQQLLHELHVRIDVAAPLSRYPLAIQQMVAISRALSISAKVLILD
EPTSSLDDAEVKLLFEVLRKLRAQGMAILFVTHFLEQTYEISDRITVLRNGQLEGEYLAS
QLSRLDLVNKMVGSQTSSSPNLDAADGRAEPQVDSGQTAVLQARGLGRNGILAPLDLDLR
GGEVLGLCGLLGSGRTETARLLFGADKPDSGTLQIKGKVEKFHSPRDAIAAGIGFCSEDR
KKEGAILELSVRENIVLALQARAGLLRVIPRRRQQALASDYVKWLGIKTADIETPIGSLS
GGNQQKALLARWLATDPAMLILDEPTRGIDVRAKQEIMEHVIALCRKGMAILFISSEISE
VLRVSDRMLVLRDRKACGEYLRGELDEDSVLQVIAGEGA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory