SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_061941787.1 NCBI__GCF_001584185.1:WP_061941787.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
29% identity, 89% coverage: 33:301/301 of query aligns to 12:280/281 of P94530

query
sites
P94530

R

R

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W

A

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K

K

D180 (= D201) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
27% identity, 69% coverage: 93:301/301 of query aligns to 82:295/296 of P68183

query
sites
P68183

M

L

L

L

Y

W

F

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W

N

N

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K

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K

E190 (≠ D196) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A198) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G202) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P215) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
40% identity, 20% coverage: 159:219/301 of query aligns to 349:410/490 of 4ki0F

query
sites
4ki0F

D

D

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P

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P

binding alpha-D-glucopyranose: N361 (≠ Y171), G365 (= G175), Y368 (≠ L178)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 310, 418, 422

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
40% identity, 20% coverage: 159:219/301 of query aligns to 364:425/514 of P02916

query
sites
P02916

D

D

L

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P

L372 (= L167) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ Y171) mutation N->K,H: No growth on maltose.
G380 (= G175) mutation G->C,S: No growth on maltose.
E401 (≠ D196) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A198) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G202) mutation G->A,P: No effect.
P420 (= P215) mutation to A: No effect.

Sites not aligning to the query:

334 L→W: Ability to transport lactose in a saturable manner.

Query Sequence

>WP_061941787.1 NCBI__GCF_001584185.1:WP_061941787.1
MTSISSLSSAASSAPAPTPVPLFARSRFANLGRIWVHVVLCAYAVIALFPIALILINSVK
SRDAIFDNPLAFPTPDSFSLIGFEKVLHNTNFMLYFGNSLVVTLGSLVLIVLFGAMAGWA
LSEYKFRGNRLMALYLALGIMIPIRLGTVSILQLVVSLDLINTRTALILVYTAQGLPLAV
MILSEFIRQIPKELKDAARCDGVGEFKIFFQIILPLIRPAIATVAVFTMIPAWNDLWFPL
ILAPSDETKTVTLGVQQFIGQYVTDWNSVLAALSLAVIPILIMYVIFSRQLIRGLTSGAV
K

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory