SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_061942382.1 NCBI__GCF_001584185.1:WP_061942382.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q97U29 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase; 2-dehydro-3-deoxyglucono/galactono-kinase; 2-keto-3-deoxy-galactonokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; KDGal kinase; EC 2.7.1.178 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
32% identity, 87% coverage: 3:268/306 of query aligns to 2:273/313 of Q97U29

query
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Q97U29

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GSELN 34:38 (≠ GDTSN 31:35) binding substrate
Y90 (= Y87) binding substrate
YYR 106:108 (≠ YLR 100:102) binding substrate
NIR 164:166 (≠ NLR 162:164) binding ATP
R166 (= R164) binding substrate
KLGSKG 226:231 (≠ KLGAEG 221:226) binding ATP
GAGD 255:258 (= GAGD 250:253) binding ATP
D258 (= D253) binding substrate

Sites not aligning to the query:

294 binding substrate

2varA Crystal structure of sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate (see paper)
32% identity, 87% coverage: 3:268/306 of query aligns to 1:272/311 of 2varA

query
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2varA

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active site: G254 (= G250), A255 (= A251), G256 (= G252), D257 (= D253)
binding adenosine monophosphate: G227 (= G223), G230 (= G226), M259 (≠ F255)
binding phosphoaminophosphonic acid-adenylate ester: K225 (= K221), G227 (= G223), S228 (≠ A224), G230 (= G226), G254 (= G250), A255 (= A251), G256 (= G252), D257 (= D253), M259 (≠ F255)
binding 3-deoxy-alpha-D-erythro-hex-2-ulofuranosonic acid: L10 (≠ M12), G33 (= G31), S34 (≠ D32), Y89 (= Y87), Y105 (= Y100), R107 (= R102), I136 (= I131), R165 (= R164), G254 (= G250), D257 (= D253)
binding 2-keto-3-deoxygluconate: G33 (= G31), S34 (≠ D32), Y89 (= Y87), L103 (≠ F98), Y105 (= Y100), R107 (= R102), I136 (= I131), R165 (= R164), T253 (= T249), G254 (= G250), D257 (= D253)

Sites not aligning to the query:

2dcnA Crystal structure of 2-keto-3-deoxygluconate kinase from sulfolobus tokodaii complexed with 2-keto-6-phosphogluconate (alpha-furanose form)
30% identity, 98% coverage: 5:303/306 of query aligns to 3:305/308 of 2dcnA

query
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2dcnA

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active site: G252 (= G250), A253 (= A251), G254 (= G252), D255 (= D253)
binding adenosine-5'-diphosphate: D193 (≠ S190), K223 (= K221), G225 (= G223), P226 (≠ A224), G228 (= G226), V247 (≠ A245), G254 (= G252), I279 (≠ N277), S282 (≠ A280), V286 (≠ T284)
binding 6-O-phosphono-beta-D-psicofuranosonic acid: G33 (= G31), F89 (≠ Y87), Y105 (= Y100), R107 (= R102), I136 (= I131), R165 (= R164), T251 (= T249), G252 (= G250), D255 (= D253), D291 (≠ A289)

5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
30% identity, 94% coverage: 9:297/306 of query aligns to 8:299/306 of 5eynA

query
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G

F

L

I

L

A

Y

R

R

I

L

V

S

A

V

G

A

D

Q

D

D

-

W

-

H

-

N

-

Q

-

A

-

T

P

I

F

L

R

D

A

A

A

V

R

K

Y

W

A

A

N
|
N

V

G

C

C

A
x
G

A
|
A

L

L

S

A

T

T

G

Q

Y

K

G

G

A

A

V

M

A

T

P

A

I

L

P

P

H

N

A

Q

A

A

active site: G246 (= G250), A247 (= A251), G248 (= G252), D249 (= D253)
binding adenosine-5'-diphosphate: H91 (≠ D94), T217 (≠ K221), G219 (= G223), A220 (= A224), A238 (≠ S242), V239 (= V243), T244 (≠ A248), G246 (= G250), A247 (= A251), G248 (= G252), F251 (= F255), N279 (= N277), G282 (≠ A280), A283 (= A281)
binding beryllium trifluoride ion: G246 (= G250), G248 (= G252), D249 (= D253)
binding beta-D-fructofuranose: D9 (≠ E10), D13 (≠ E14), G28 (= G31), A29 (≠ D32), N32 (= N35), F96 (= F98), F98 (≠ Y100), R159 (= R164), D249 (= D253)

5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
30% identity, 94% coverage: 9:297/306 of query aligns to 12:303/310 of 5yggA

query
sites
5yggA

G

G

E
x
D

A

A

M

V

V

V

E
x
D

F

L

-

I

-

P

N

D

Q

G

R

Q

Q

Q

H

H

D

-

T

-

R

-

M

-

Y

Y

L

L

Q

K

G

C

F

P

G

G

G
|
G

D
x
A

T

P

S

A

N

N

F

V

C

A

I

V

A

A

A

I

A

A

R

R

Q

L

G

S

A

G

R

R

S

S

G

A

Y

F

I

F

S

G

A

R

L

V

G

G

N

N

D

D

H

P

F

F

G

G

E

R

Q

F

L

M

R

Q

A

Q

L

T

W

L

Q

T

A

D

E

E

Q

Q

V

V

D

D

A

C

S

Q

H

H

V

L

A

H

R

F

D

D

A

P

N

V

A

H

A

R

T

T

G

S

V

T

Y

V

F

V

V

V

S

D

H

L

D

D

Q

E

D

C

G

G

H

E

H

R

-

S

F
|
F

D

T

Y
x
F

L

M

R

V

A

K

G

P

S

S

A

A

A

D

S

Q

R

F

Y

L

S

Q

S

L

E

S

Q

D

L

I

P

P

L

-

A

-

A

S

I

F

A

Q

A

K

A

G

K

E

V

W

L

L

H

H

L

V

S

C

G

S

I

I

S

A

L

L

A

A

I

N

S

Q

A

P

S

S

A

R

C

-

D

S

A

S

G

T

L

F

A

A

M

I

A

A

Y

Q

A

M

R

K

K

E

H

V

G

G

V

G

K

Y

T

V

S

S

L

F

D

D

T

P

N

N

L

L

R
|
R

L

E

K

E

L

V

W

W

-

S

P

E

L

P

E

Q

R

E

A

L

Q

Q

E

A

K

T

I

V

R

M

A

R

A

A

F

V

A

G

L

L

C

A

D

D

I

V

C

V

L
x
K

P

F

S

S

W

E

D

E

I

L

S

Q

A

F

L

L

T

T

S

G

L

T

D

Q

D

S

R

I

D

E

A

E

I

G

V

L

D

Q

E

A

L

I

L

A

S

D

Y

F

G

Q

I

I

G

P

L

L

I

V

A

V

F

V

K
x
T

L

L

G
|
G

A

A

E

K

G

G

C

A

Y

L

V

V

A

A

T

T

A

P

K

N

E

S

R

Q

L

I

V

V

P

S

P

G

Y

K

S
x
A

V
|
V

E

K

A

P

I

I

D

D

A

C

T

T

G

G

A

A

G

G

D
|
D

C

A

F
|
F

G

V

G

G

A

G

F

L

I

L

A

Y

R

R

I

L

V

S

A

V

G

A

D

Q

D

D

-

W

-

H

-

N

-

Q

-

A

-

T

P

I

F

L

R

D

A

A

A

V

R

K

Y

W

A

A

N
|
N

V

G

C

C

A
x
G

A
|
A

L

L

S

A

T

T

G

Q

Y

K

G

G

A

A

V

M

A

T

P

A

I

L

P

P

H

N

A

Q

A

A

binding adenosine-5'-diphosphate: K188 (≠ L188), T221 (≠ K221), G223 (= G223), A242 (≠ S242), V243 (= V243), F255 (= F255), N283 (= N277), G286 (≠ A280), A287 (= A281)
binding beta-D-fructofuranose: D13 (≠ E10), D17 (≠ E14), G32 (= G31), A33 (≠ D32), F100 (= F98), F102 (≠ Y100), R163 (= R164), D253 (= D253)

3iq0B Crystal structure of a putative ribokinase ii in complex with atp and mg+2 from e.Coli
30% identity, 88% coverage: 36:305/306 of query aligns to 41:307/308 of 3iq0B

query
sites
3iq0B

F

F

C

I

I

D

A

Q

A

V

A

T

R

R

Q

L

G

G

A

V

R

P

S

C

G

G

Y

I

I

I

S

S

A

C

L

V

G

G

N

N

D

D

H

G

F

F

G

G

E

D

Q

I

L

N

R

I

A

H

L

R

W

L

Q

A

A

A

E

D

Q

G

V

V

D

D

A

I

S

R

H

G

V

I

A

S

R

V

D

L

A

P

N

L

A

E

A

A

T

T

G

G

V

S

Y

A

F

F

V

V

S

T

H

Y

-

G

D

D

Q

R

D

D

G

-

H

-

F

F

D

I

Y

F

L

N

R

I

A

K

G

N

S

A

A

A

A

C

S

G

R

K

Y

L

S

S

S

A

E

Q

Q

H

L

V

P

D

L

E

A

N

A

I

I

L

A

K

A

D

A

C

K

T

V

H

L

F

H

H

L

I

S

M

G

G

I

S

S

S

L

L

A

-

I

F

S

S

A

F

S

H

A

M

C

V

D

D

A

A

G

V

L

K

A

K

A

A

M

V

A

T

Y

I

A

V

R

K

K

A

H

N

G

G

V

G

K

V

T

I

S

S

L

F

D

D

T

P

N

N

L

I

R

R

L

K

K

E

L

M

W

L

P

D

L

I

E

P

R

E

A

M

Q

R

E

D

K

A

I

L

R

H

A

F

A

V

F

L

A

E

L

L

C

T

D

D

I

I

C

Y

L

M

P

P

S
|
S

W

E

D

G

D

E

I

V

S

L

A

L

L

L

T

S

S

P

L

H

D

S

D

T

R

P

D

E

A

R

I

A

V

I

D

A

E

G

L

F

L

L

S

E

Y

E

G

G

I

V

G

K

L

E

I

V

A

I

F

V

K
|
K

L

R

G
|
G

A

N

E

Q

G

G

C

A

Y

S

V

Y

A

Y

T

S

A

A

K

N

E

E

R

Q

R

F

L

H

V

V

P

E

P

S

Y

Y

S

P

V

V

E

E

A
x
E

I

V

D

D

A

P

T

T

G
|
G

A
|
A

G
|
G

D
|
D

C

C

F
|
F

G

G

A

A

F

W

I

I

A

A

R

C

I

R

V

Q

A

L

G

G

D

F

D

D

P

A

F

H

R

R

A

A

A

L

R

Q

Y

Y

A

A

N
|
N

V

A

C

C

A
x
G

A

A

L

L

S

A

T

V

T

T

G

R

Y

R

G

G

A

P

V

M

A

E

P

G

I

T

P

S

H

R

A

L

A

M

Q

E

V

I

E

E

E

T

I

F

L

I

P

Q

R

R

active site: G252 (= G250), A253 (= A251), G254 (= G252), D255 (= D253)
binding adenosine-5'-triphosphate: S192 (= S190), K223 (= K221), G225 (= G223), E247 (≠ A245), A253 (= A251), G254 (= G252), F257 (= F255), N279 (= N277), G282 (≠ A280)

1v1aA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
28% identity, 99% coverage: 2:303/306 of query aligns to 1:301/301 of 1v1aA

query
sites
1v1aA

T

M

I

L

D

E

I

V

L

V

A

T

Y

A

G

G

E

E

A

P

M
x
L

V

V

E

A

F

L

N

V

Q

P

R

Q

Q

E

-

P

-

G

-

H

-

L

H

R

D

G

T

K

R

R

M

L

Y

L

L

E

Q

V

G

Y

F

V

G

G

G
|
G

D
x
A

T

E

S

V

N
|
N

F

V

C

A

I

V

A

A

A

L

A

A

R

R

Q

L

G

G

A

V

R

K

S

V

G

G

Y

F

I

V

S

G

A

R

L

V

G

G

N

E

D

D

H

E

F

L

G

G

E

A

Q

M

L

V

R

E

A

E

L

R

W

L

Q

R

A

A

E

E

Q

G

V

V

D

D

A

L

S

T

H

H

V

F

A

-

R

R

D

R

A

A

N

P

A

G

A

F

T

T

G

G

V

L

Y
|
Y

F

L

V

R

S

E

H

Y

D

L

Q

P

D

L

G

G

H

Q

-

G

H

R

F

V

D

F

Y

Y

L

Y

R
|
R

A

K

G

G

S

S

A

A

A

G

S

S

R

A

Y

L

S

A

S

P

E

G

Q

A

L

F

P

D

L

P

A

D

A

Y

I

L

A

E

A

G

A

V

K

R

V

F

L

L

H

H

L

L

S

S

G

G

I

I

S

T

L

P

A

A

I

L

S

S

A

P

S

E

A

A

C

R

D

A

A

F

G

S

L

L

A

W

A

A

M

M

A

E

Y

E

A

A

R

K

K

R

H

R

G

G

V

V

K

R

T

V

S

S

L

L

D

D

T

V

N

N

L

Y

R
|
R

L

Q

K

T

L

L

W

W

P

S

L

P

E

E

R

E

A

A

Q

R

E

G

K

F

I

L

R

E

A

R

A

A

F

L

A

P

L

G

C

V

D

D

I

L

C

L

L

F

P

L

S

S

W

E

D

E

I

A

S

E

A

L

L

L

T

F

S

G

L

-

D

-

R

-

D

-

A

R

I

V

V

E

D

E

E

A

L

L

L

R

S

A

Y

L

G

S

I

A

G

P

L

E

I

V

A

V

F

L

K
|
K

L

R

G
|
G

A
|
A

E

K

G

G

C

A

Y

W

V

A

A

F

T

V

A

D

K

G

E

R

R

R

R

V

L

E

V

G

P

S

P

A

Y

F

S

A

V

V

E

E

A

A

I

V

D

D

A

P

T

V

G
|
G

A
|
A

G
|
G

D
|
D

C

A

F

F

G

A

A

G

F

Y

I

L

A

A

R

G

I

A

V

V

A

W

G

G

D

L

D

P

P

V

F

E

R

E

A

R

A

L

R

R

Y

L

A

A

N
|
N

V

L

C

L

A

G

A
|
A

L

S

S

V

T

A

G

S

Y

R

G

G

A
x
D

V

H

A

E

P

G

I

A

P

P

H

Y

A

R

A

E

Q

D

V

L

E

E

E

V

I

L

L

L

active site: G248 (= G250), A249 (= A251), G250 (= G252), D251 (= D253)
binding adenosine-5'-diphosphate: K219 (= K221), G221 (= G223), A222 (= A224), A249 (= A251), G250 (= G252), N275 (= N277), A279 (= A281)
binding 2-keto-3-deoxygluconate: L11 (≠ M12), G34 (= G31), A35 (≠ D32), N38 (= N35), Y89 (= Y87), R105 (= R102), R167 (= R164), G248 (= G250), D251 (= D253), D287 (≠ A289)

Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
28% identity, 98% coverage: 3:303/306 of query aligns to 2:301/309 of Q53W83

query
sites
Q53W83

I

L

D

E

I

V

L

V

A

T

Y

A

G

G

E

E

A

P

M

L

V

V

E

A

F

L

N

V

Q

P

R

Q

Q

E

-

P

-

G

-

H

-

L

H

R

D

G

T

K

R

R

M

L

Y

L

L

E

Q

V

G

Y

F

V

G

G

G
|
G

D
x
A

T
x
E

S
x
V

N
|
N

F

V

C

A

I

V

A

A

A

L

A

A

R

R

Q

L

G

G

A

V

R

K

S

V

G

G

Y

F

I

V

S

G

A

R

L

V

G

G

N

E

D

D

H

E

F

L

G

G

E

A

Q

M

L

V

R

E

A

E

L

R

W

L

Q

R

A

A

E

E

Q

G

V

V

D

D

A

L

S

T

H

H

V

F

A

-

R

R

D

R

A

A

N

P

A

G

A

F

T

T

G

G

V

L

Y

Y

F

L

V

R

S

E

H

Y

D

L

Q

P

D

L

G

G

H

Q

-

G

H

R

F

V

D

F

Y
|
Y

L
x
Y

R
|
R

A

K

G

G

S

S

A

A

A

G

S

S

R

A

Y

L

S

A

S

P

E

G

Q

A

L

F

P

D

L

P

A

D

A

Y

I

L

A

E

A

G

A

V

K

R

V

F

L

L

H

H

L

L

S

S

G

G

I

I

S

T

L

P

A

A

I

L

S

S

A

P

S

E

A

A

C

R

D

A

A

F

G

S

L

L

A

W

A

A

M

M

A

E

Y

E

A

A

R

K

K

R

H

R

G

G

V

V

K

R

T

V

S

S

L

L

D

D

T

V

N

N

L

Y

R
|
R

L

Q

K

T

L

L

W

W

P

S

L

P

E

E

R

E

A

A

Q

R

E

G

K

F

I

L

R

E

A

R

A

A

F

L

A

P

L

G

C

V

D

D

I

L

C

L

L

F

P

L

S
|
S

W

E

D

E

I

A

S

E

A

L

L

L

T

F

S

G

L

-

D

-

R

-

D

-

A

R

I

V

V

E

D

E

E

A

L

L

L

R

S

A

Y

L

G

S

I

A

G

P

L

E

I

V

A

V

F

L

K
|
K

L
x
R

G
|
G

A
|
A

E
x
K

G
|
G

C
x
A

Y

W

V

A

A

F

T

V

A

D

K

G

E

R

R

R

R

V

L

E

V

G

P

S

P

A

Y

F

S

A

V

V

E

E

A

A

I

V

D

D

A

P

T

V

G
|
G

A
|
A

G
|
G

D
|
D

C

A

F

F

G

A

A

G

F

Y

I

L

A

A

R

G

I

A

V

V

A

W

G

G

D

L

D

P

P

V

F

E

R

E

A

R

A

L

R

R

Y

L

A

A

N
|
N

V

L

C

L

A

G

A

A

L

S

S

V

T

A

G

S

Y

R

G

G

A
x
D

V

H

A

E

P

G

I

A

P

P

H

Y

A

R

A

E

Q

D

V

L

E

E

E

V

I

L

L

L

GAEVN 34:38 (≠ GDTSN 31:35) binding substrate
YYR 103:105 (≠ YLR 100:102) binding substrate
R167 (= R164) binding substrate
S193 (= S190) binding ATP
219:225 (vs. 221:227, 57% identical) binding ATP
GAGD 248:251 (= GAGD 250:253) binding ATP
D251 (= D253) binding substrate
N275 (= N277) binding ATP
D287 (≠ A289) binding substrate

1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp (see paper)
29% identity, 92% coverage: 2:281/306 of query aligns to 1:279/300 of 1v1bA

query
sites
1v1bA

T

M

I

L

D

E

I

V

L

V

A

T

Y

A

G

G

E

E

A

P

M

L

V

V

E

A

F

L

N

V

Q

P

R

Q

Q

E

-

P

-

G

-

H

-

L

H

R

D

G

T

K

R

R

M

L

Y

L

L

E

Q

V

G

Y

F

V

G

G

D

A

T

E

S

V

N

N

F

V

C

A

I

V

A

A

A

L

A

A

R

R

Q

L

G

G

A

V

R

K

S

V

G

G

Y

F

I

V

S

G

A

R

L

V

G

G

N

E

D

D

H

E

F

L

G

G

E

A

Q

M

L

V

R

E

A

E

L

R

W

L

Q

R

A

A

E

E

Q

G

V

V

D

D

A

L

S

T

H

H

V

F

A

-

R

R

D

R

A

A

N

P

A

G

A

F

T

T

G

G

V

L

Y

Y

F

L

V

R

S

E

H

Y

D

L

Q

P

D

L

G

G

H

Q

-

G

H

R

F

V

D

F

Y

Y

L

Y

R

R

A

K

G

G

S

S

A

A

A

G

S

S

R

A

Y

L

S

A

S

P

E

G

Q

A

L

F

P

D

L

P

A

D

A

Y

I

L

A

E

A

G

A

V

K

R

V

F

L

L

H

H

L

L

S

S

G

G

I

I

S

T

L

P

A

A

I

L

S

S

A

P

S

E

A

A

C

R

D

A

A

F

G

S

L

L

A

W

A

A

M

M

A

E

Y

E

A

A

R

K

K

R

H

R

G

G

V

V

K

R

T

V

S

S

L

L

D

D

T

V

N

N

L

Y

R

R

L

Q

K

T

L

L

W

W

P

S

L

P

E

E

R

E

A

A

Q

R

E

G

K

F

I

L

R

E

A

R

A

A

F

L

A

P

L

G

C

V

D

D

I

L

C

L

L

F

P

L

S

S

W

E

D

E

I

A

S

E

A

L

L

L

T

F

S

G

L

-

D

-

R

-

D

-

A

R

I

V

V

E

D

E

E

A

L

L

L

R

S

A

Y

L

G

S

I

A

G

P

L

E

I

V

A

V

F

L

K
|
K

L

R

G
|
G

A

A

E

K

G

G

C

A

Y

W

V

A

A

F

T

V

A

D

K

G

E

R

R

R

R

V

L

E

V

G

P

S

P
x
A

Y
x
F

S

A

V
|
V

E

E

A

A

I

V

D

D

A

P

T

V

G
|
G

A
|
A

G
|
G

D
|
D

C

A

F

F

G

A

A

G

F

Y

I

L

A

A

R

G

I

A

V

V

A

W

G

G

D

L

D

P

P

V

F

E

R

E

A

R

A

L

R

R

Y

L

A

A

N
|
N

V

L

C

L

A

G

A
|
A

active site: G248 (= G250), A249 (= A251), G250 (= G252), D251 (= D253)
binding adenosine-5'-triphosphate: K219 (= K221), G221 (= G223), A238 (≠ P240), F239 (≠ Y241), V241 (= V243), G248 (= G250), A249 (= A251), G250 (= G252), N275 (= N277), A279 (= A281)

Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
29% identity, 98% coverage: 5:303/306 of query aligns to 7:307/319 of Q8ZKR2

query
sites
Q8ZKR2

I

V

L

W

A

V

Y

I

G

G

E

D

A

A

M

S

V

V

E
x
D

F

L

N

V

Q

P

R

E

Q

K

H

Q

D

N

T

S

R

-

M

-

Y

Y

L

L

Q

K

G

C

F

P

G

G

G
|
G

D

A

T

S

S

A

N

N

F

V

C

G

I

V

A

C

A

V

A

A

R

R

Q

L

G

G

A

G

R

E

S

C

G

G

Y

F

I

I

S

G

A

C

L

L

G

G

N

D

D

D

H

D

F

A

G

G

E

R

Q

F

L

L

R

R

A

Q

L

V

W

F

Q

Q

A

D

E

N

Q

G

V

V

D

D

A

V

S

T

H

F

V

L

A

R

R

L

D

D

A

A

N

D

A

L

A

T

T

S

G

A

V

V

Y

L

F

I

V

V

S

N

H

L

D

T

Q

A

D

D

G

G

H

E

H

R

-

S

F

F

D

T

Y
|
Y

L

L

R

V

A

H

G

P

S

G

A

A

A

D

S

T

R

Y

Y

V

S

S

S

P

E

Q

Q

D

L

L

P

P

L

-

A

-

A

P

I

F

A

R

A

Q

A

Y

K

E

V

W

L

F

H

Y

L

F

S

S

G

S

I

I

S

G

L

L

A

T

I

-

S

D

A

R

S

P

A

A

C

R

D

E

A

A

G

C

L

L

A

E

A

G

M

A

A

R

Y

R

A

M

R

R

K

E

H

A

G

G

V

G

K

Y

T

V

S

L

L

F

D

D

T

V

N

N

L

L

R
|
R

L

S

K

K

L

M

W

W

-

G

P

N

L

T

E

D

R

E

A

I

Q

P

E

E

K

L

I

I

R

A

A

R

A

S

F
x
A

A
|
A

L

L

C
x
A

D

S

I

I

C

C

L

K

P

V

S

S

W

A

D

D

D

E

I

L

S

C

A

Q

L

L

T

S

S

G

L

A

D

S

D

H

R

W

D

Q

A

D

I

A

V

R

D

Y

E

Y

L

L

L

R

S

D

Y

L

G
|
G

I

C

G

D

L

T

I

T

A

I

F

I

K

S

L

L

G

G

A

A

E

D

G

G

C

A

Y

L

V

L

A

I

T

T

A

A

K

E

E

G

R

E

R

F

L

H

V

F

P

P

P

A

Y

P

S

R

V

V

E

D

A

V

I

V

D
|
D

A

T

T
|
T

G

G

A

A

G

G

D
|
D

C

A

F

F

G

V

G

G

A

G

F

L

-

L

-

F

-

T

I

L

A

S

R

R

I

A

V

N

A

C

G

W

D

D

D

H

P

A

F

L

-

L

-

A

R

E

A

A

A

I

R

S

Y

N

A

A

N

N

V

A

C

C

A

G

A

A

L

M

S
x
A

T

V

T

T

G
x
A

Y

K

G
|
G

A

A

V

M

A

T

P

A

I

L

P

P

H

F

A

P

A

D

Q

Q

V

L

E

N

E

T

I

F

L

L

D16 (≠ E14) binding 5-amino-1-(beta-D-ribosyl)imidazole
G31 (= G31) binding 5-amino-1-(beta-D-ribosyl)imidazole
Y101 (= Y100) binding 5-amino-1-(beta-D-ribosyl)imidazole
R162 (= R164) binding 5-amino-1-(beta-D-ribosyl)imidazole
A180 (≠ F181) binding K(+)
A181 (= A182) binding K(+)
A183 (≠ C184) binding K(+)
G213 (= G214) binding K(+)
D246 (= D247) binding K(+)
T248 (= T249) binding K(+)
D252 (= D253) binding 5-amino-1-(beta-D-ribosyl)imidazole
A287 (≠ S283) binding K(+)
A290 (≠ G286) binding K(+)
G292 (= G288) binding K(+)

1tz3A Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
29% identity, 98% coverage: 5:303/306 of query aligns to 3:296/299 of 1tz3A

query
sites
1tz3A

I

V

L

W

A

V

Y

I

G

G

E
x
D

A

A

M
x
S

V

V

E
x
D

F

L

N

V

Q

P

R

E

Q

K

H

Q

D

N

T

S

R

-

M

-

Y

Y

L

L

Q

K

G
x
C

F

P

G

G

G
|
G

D

A

T

S

S

A

N

N

F

V

C

G

I

V

A

C

A

V

A

A

R

R

Q

L

G

G

A

G

R

E

S

C

G

G

Y

F

I

I

S

G

A

C

L

L

G

G

N

D

D

D

H

D

F

A

G

G

E

R

Q

F

L

L

R

R

A

Q

L

V

W

F

Q

Q

A

D

E

N

Q

G

V

V

D

D

A

V

S

T

H

F

V

L

A

R

R

L

D

D

A

A

N

D

A

L

A

T

T

S

G

A

V

V

Y
x
L

F

I

V

V

S

-

H

-

D

-

Q

-

D

-

G

-

H

N

H

S

F
|
F

D

T

Y
|
Y

L

L

R

V

A

H

G

P

S

G

A

A

A

D

S

T

R

Y

Y

V

S

S

S

P

E

Q

Q

D

L

L

P

P

L

-

A

-

A

P

I

F

A

R

A

Q

A

Y

K

E

V

W

L

F

H

Y

L

F

S

S

G

S

I

I

S

G

L

L

A

T

I

-

S

D

A

R

S

P

A

A

C

R

D

E

A

A

G

C

L

L

A

E

A

G

M

A

A

R

Y

R

A

M

R

R

K

E

H

A

G

G

V

G

K

Y

T

V

S

L

L

F

D

D

T

V

N

N

L

L

R
|
R

L

S

K

K

L
x
M

W

W

-

G

P

N

L

T

E

D

R

E

A

I

Q

P

E

E

K

L

I

I

R

A

A

R

A

S

F

A

A

A

L

L

C

A

D

S

I

I

C

C

L

K

P

V

S

S

W

A

D

D

D

E

I

L

S

C

A

Q

L

L

T

S

S

G

L

A

D

S

D

H

R

W

D

Q

A

D

I

A

V

R

D

Y

E

Y

L

L

L

R

S

D

Y

L

G

G

I

C

G

D

L

T

I

T

A

I

F

I

K

S

L

L

G

G

A

A

E

D

G

G

C

A

Y

L

V

L

A

I

T

T

A

A

K

E

E

G

R

E

R

F

L

H

V

F

P

P

P

A

Y

P

S

R

V

V

E

D

A

V

I

V

D

D

A

T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

C

A

F

F

G

V

G

G

A

G

F

L

-

L

-

F

-

T

I

L

A

S

R

R

I

A

V

N

A

C

G

W

D

D

D

H

P

A

F

L

-

L

-

A

R

E

A

A

A

I

R

S

Y

N

A

A

N

N

V

A

C

C

A

G

A

A

L

M

S

A

T

V

T

T

G

A

Y

K

G

G

A

A

V

M

A

T

P

A

I

L

P

P

H

F

A

P

A

D

Q

Q

V

L

E

N

E

T

I

F

L

L

active site: C24 (≠ G28), F88 (= F98), G238 (= G250), A239 (= A251), G240 (= G252), D241 (= D253)
binding 5-aminoimidazole ribonucleoside: D8 (≠ E10), S10 (≠ M12), D12 (≠ E14), G27 (= G31), L83 (≠ Y87), F88 (= F98), Y90 (= Y100), R151 (= R164), M154 (≠ L167), D241 (= D253)

1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog (see paper)
29% identity, 98% coverage: 5:303/306 of query aligns to 3:296/297 of 1tz6A

query
sites
1tz6A

I

V

L

W

A

V

Y

I

G

G

E
x
D

A

A

M

S

V

V

E
x
D

F

L

N

V

Q

P

R

E

Q

K

H

Q

D

N

T

S

R

-

M

-

Y

Y

L

L

Q

K

G
x
C

F

P

G

G

G
|
G

D

A

T

S

S

A

N

N

F

V

C

G

I

V

A

C

A

V

A

A

R

R

Q

L

G

G

A

G

R

E

S

C

G

G

Y

F

I

I

S

G

A

C

L

L

G

G

N

D

D

D

H

D

F

A

G

G

E

R

Q

F

L

L

R

R

A

Q

L

V

W

F

Q

Q

A

D

E

N

Q

G

V

V

D

D

A

V

S

T

H

F

V

L

A

R

R

L

D

D

A

A

N

D

A

L

A

T

T

S

G

A

V

V

Y

L

F

I

V

V

S

-

H

-

D

-

Q

-

D

-

G

-

H

N

H

S

F
|
F

D

T

Y
|
Y

L

L

R

V

A

H

G

P

S

G

A

A

A

D

S

T

R

Y

Y

V

S

S

S

P

E

Q

Q

D

L

L

P

P

L

-

A

-

A

P

I

F

A

R

A

Q

A

Y

K

E

V

W

L

F

H

Y

L

F

S

S

G

S

I

I

S

G

L

L

A

T

I

-

S

D

A

R

S

P

A

A

C

R

D

E

A

A

G

C

L

L

A

E

A

G

M

A

A

R

Y

R

A

M

R

R

K

E

H

A

G

G

V

G

K

Y

T

V

S

L

L

F

D

D

T

V

N
|
N

L

L

R
|
R

L

S

K

K

L
x
M

W

W

-

G

P

N

L

T

E

D

R

E

A

I

Q

P

E

E

K

L

I

I

R

A

A

R

A

S

F

A

A

A

L

L

C

A

D

S

I

I

C

C

L
x
K

P

V

S

S

W

A

D

D

D
x
E

I

L

S

C

A

Q

L

L

T

S

S

G

L

A

D

S

D

H

R

W

D

Q

A

D

I

A

V

R

D

Y

E

Y

L

L

L

R

S

D

Y

L

G

G

I

C

G

D

L

T

I

T

A

I

F

I

K
x
S

L

L

G
|
G

A
|
A

E

D

G
|
G

C

A

Y

L

V

L

A

I

T

T

A

A

K

E

E

G

R

E

R

F

L

H

V

F

P

P

P

A

Y

P

S

R

V

V

E

D

A

V

I

V

D

D

A

T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

C

A

F
|
F

G

V

G

G

A

G

F

L

-

L

-

F

-

T

I

L

A

S

R

R

I

A

V

N

A

C

G

W

D

D

D

H

P

A

F

L

-

L

-

A

R

E

A

A

A

I

R

S

Y

N

A

A

N
|
N

V

A

C

C

A
x
G

A
|
A

L

M

S

A

T

V

T

T

G

A

Y

K

G

G

A

A

V

M

A

T

P

A

I

L

P

P

H

F

A

P

A

D

Q

Q

V

L

E

N

E

T

I

F

L

L

active site: C24 (≠ G28), F88 (= F98), G238 (= G250), A239 (= A251), G240 (= G252), D241 (= D253)
binding phosphomethylphosphonic acid adenylate ester: N149 (= N162), K176 (≠ L188), E181 (≠ D193), S209 (≠ K221), G211 (= G223), A212 (= A224), G214 (= G226), A239 (= A251), G240 (= G252), F243 (= F255), N270 (= N277), G273 (≠ A280), A274 (= A281)
binding 5-aminoimidazole ribonucleoside: D8 (≠ E10), D12 (≠ E14), G27 (= G31), F88 (= F98), Y90 (= Y100), R151 (= R164), M154 (≠ L167), D241 (= D253)

3gbuA Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with atp
29% identity, 93% coverage: 5:289/306 of query aligns to 2:283/302 of 3gbuA

query
sites
3gbuA

I

I

L

A

A

S

Y

I

G

G

E

E

A

L

M

L

V

I

E

D

F

L

N

I

Q

S

R

V

Q

E

H

E

-

G

-

D

-

L

-

K

D

D

T

V

R

R

M

L

Y

F

L

E

Q

K

G

H

F

P

G

G

D

A

T

P

S

A

N

N

F

V

C

A

I

V

A

G

A

V

A

S

R

R

Q

L

G

G

A

V

R

K

S

S

G

S

Y

L

I

I

S

S

A

K

L

V

G

G

N

N

D

D

H

P

F

F

G

G

E

E

Q

Y

L

L

R

I

A

E

L

E

W

L

Q

S

A

K

E

E

Q

N

V

V

D

D

A

T

S

R

H

G

V

I

A

V

R

K

D

D

A

E

N

K

A

K

A

H

T

T

G

G

V

I

Y

V

F

F

V

V

S

Q

H

L

D

K

Q

G

D

A

G

S

H

P

H

S

F

F

D

-

Y

L

L

L

R

Y

A

D

G

D

S

V

A

A

A

Y

S

F

R

N

Y

M

S

T

S

L

E

N

Q

D

L

I

P

N

L

W

A

D

A

I

I

V

A

E

A

A

K

K

V

I

L

V

H

N

L

F

S

G

G

S

I

V

S

I

L

L

A

A

I

R

S

N

A

P

S

S

A

R

C

E

D

-

A

-

G

-

L

-

A

T

A

V

M

M

A

K

Y

V

A

I

R

K

K

K

H

-

G

-

V

I

K

K

T

G

S

S

-

S

-

L

-

I

-

A

L

F

D

D

T

V

N

N

L

L

R

R

L

L

K

D

L

L

W

W

P

-

L

-

E

-

R

R

A

G

Q

Q

E

E

K

E

-

E

-

M

-

I

-

K

-

V

I

L

R

E

A

E

A

S

F

I

A

K

L

L

C

A

D

D

I

I

C

V

L
x
K

P

A

S

S

W

E

D

E

I

V

S

-

A

-

L

-

T

L

S

Y

L

L

D

E

D

N

R

Q

D

G

A

V

I

E

V

V

D

K

E

-

L

-

S

-

Y

-

G

G

I

S

G

M

L

L

I

T

A

A

F

I

K
x
T

L

L

G
|
G

A

P

E

K

G

G

C

F

Y

R

V

L

A

I

T

K

A

N

K

E

E

T

R

V

L

D

V

V

P

P

P

S

Y

Y

S

N

V
|
V

E

N

A
x
P

I

L

D

D

A

T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

C

A

F

F

G

M

G

A

A

A

F

L

I

L

A

V

R

G

I

I

-

L

-

K

V

L

A

K

G

G

D

L

D

D

P

L

F

L

R

K

A

L

A

G

R

K

Y

F

A

A

N

N

V

L

C

V

A
|
A

A

A

L

L

S

S

T

T

T

Q

G

K

Y

R

G

G

A

A

active site: G242 (= G250), A243 (= A251), G244 (= G252), D245 (= D253)
binding adenosine-5'-triphosphate: K188 (≠ L188), T213 (≠ K221), G215 (= G223), V235 (= V243), P237 (≠ A245), A243 (= A251), G244 (= G252), A274 (= A280)

3ih0A Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with amp-pnp
29% identity, 93% coverage: 5:289/306 of query aligns to 3:284/304 of 3ih0A

query
sites
3ih0A

I

I

L

A

A

S

Y

I

G

G

E

E

A

L

M

L

V

I

E

D

F

L

N

I

Q

S

R

V

Q

E

H

E

-

G

-

D

-

L

-

K

D

D

T

V

R

R

M

L

Y

F

L

E

Q

K

G

H

F

P

G

G

D

A

T

P

S

A

N

N

F

V

C

A

I

V

A

G

A

V

A

S

R

R

Q

L

G

G

A

V

R

K

S

S

G

S

Y

L

I

I

S

S

A

K

L

V

G

G

N

N

D

D

H

P

F

F

G

G

E

E

Q

Y

L

L

R

I

A

E

L

E

W

L

Q

S

A

K

E

E

Q

N

V

V

D

D

A

T

S

R

H

G

V

I

A

V

R

K

D

D

A

E

N

K

A

K

A

H

T

T

G

G

V

I

Y

V

F

F

V

V

S

Q

H

L

D

K

Q

G

D

A

G

S

H

P

H

S

F

F

D

-

Y

L

L

L

R

Y

A

D

G

D

S

V

A

A

A

Y

S

F

R

N

Y

M

S

T

S

L

E

N

Q

D

L

I

P

N

L

W

A

D

A

I

I

V

A

E

A

A

K

K

V

I

L

V

H

N

L

F

S

G

G

S

I

V

S

I

L

L

A

A

I

R

S

N

A

P

S

S

A

R

C

E

D

-

A

-

G

-

L

-

A

T

A

V

M

M

A

K

Y

V

A

I

R

K

K

K

H

-

G

-

V

I

K

K

T

G

S

S

-

S

-

L

-

I

-

A

L

F

D

D

T

V

N

N

L

L

R

R

L

L

K

D

L

L

W

W

P

-

L

-

E

-

R

R

A

G

Q

Q

E

E

K

E

-

E

-

M

-

I

-

K

-

V

I

L

R

E

A

E

A

S

F

I

A

K

L

L

C

A

D

D

I

I

C

V

L

K

P

A

S

S

W

E

D

E

I

V

S

-

A

-

L

-

T

L

S

Y

L

L

D

E

D

N

R

Q

D

G

A

V

I

E

V

V

D

K

E

-

L

-

S

-

Y

-

G

G

I

S

G

M

L

L

I

T

A

A

F

I

K
x
T

L

L

G
|
G

A

P

E

K

G

G

C

F

Y

R

V

L

A

I

T

K

A

N

K

E

E

T

R

V

L

D

V

V

P

P

P

S

Y

Y

S

N

V
|
V

E

N

A

P

I

L

D

D

A

T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

C

A

F

F

G

M

G

A

A

A

F

L

I

L

A

V

R

G

I

I

-

L

-

K

V

L

A

K

G

G

D

L

D

D

P

L

F

L

R

K

A

L

A

G

R

K

Y

F

A

A

N

N

V

L

C

V

A
|
A

A

A

L

L

S

S

T

T

T

Q

G

K

Y

R

G

G

A

A

active site: G243 (= G250), A244 (= A251), G245 (= G252), D246 (= D253)
binding phosphoaminophosphonic acid-adenylate ester: T214 (≠ K221), G216 (= G223), V236 (= V243), A244 (= A251), G245 (= G252), A275 (= A280)

3in1A Crystal structure of a putative ribokinase in complex with adp from e.Coli
26% identity, 90% coverage: 30:303/306 of query aligns to 39:308/312 of 3in1A

query
sites
3in1A

G

G

D

D

T

A

S

I

N

N

F

E

C

A

I

T

A

I

A

S

R

R

Q

L

G

G

A

H

R

R

S

T

G

A

Y

L

I

M

S

S

A

R

L

I

G

G

N

K

D

D

H

A

F

A

G

G

E

Q

Q

F

L

I

R

L

A

D

L

H

W

C

Q

R

A

K

E

E

Q

N

V

I

D

D

A

I

S

Q

H

S

V

L

A

K

R

Q

D

D

A

V

N

S

A

I

A

D

T

T

G

S

V

I

Y

N

F

V

V

G

S

L

H

V

D

T

Q

E

D

D

G

G

H

E

H
x
R

F

T

D

F

Y

V

L

T

R

N

A

R

G

N

S

G

A

S

A

L

S

W

R

K

Y

L

S

N

S

I

E

D

Q

D

L

V

P

D

L

F

A

A

A

R

I

F

A

S

A

Q

A

A

K

K

V

L

L

L

H

S

L

L

S

A

G

S

I

I

-

F

-

N