SitesBLAST

F45 (≠ V35) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S44) mutation to T: Loss of ATPase activity and transport.
L60 (= L50) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ F72) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ L131) mutation to M: Loss of ATPase activity and transport.
D172 (= D168) mutation to N: Loss of ATPase activity and transport.
C276 (≠ V271) mutation to A: Lower ATPase activity and transport efficiency.
E297 (vs. gap) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
44% identity, 76% coverage: 22:296/362 of query aligns to 17:290/358 of 8y5iA

query
sites
8y5iA

I

V

L

I

K

P

G

Q

V

L

S

D

M

L

E

T

L

I

Q

N

R

N

G

G

E

E

V

F

V

L

A

T

L

L

G

G

P

P

S

S

G
|
G

S

C

G

G

K
|
K

T
|
T

L

V

L

L

R

R

A

L

V

I

A

A

G

G

L

L

E

E

S

T

P

V

K

D

S

S

G

G

S

-

I

-

Q

-

I

-

G

-

E

-

R

R

I

I

M

M

F

L

D

D

G

N

Q

E

R

D

K

I

L

T

E

H

I

V

P

P

A

A

E

E

E

N

R

R

N

Y

L

V

G

N

L

T

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

W

F

P

P

H

H

K

M

T

T

V

V

S

F

D

E

N

N

V

V

G

A

Y

F

G

G

L

L

K

R

L

M

R

Q

K

K

M

T

S

P

S

A

D

E

I

I

A

T

T

P

R

R

V

V

K

M

T

E

V

A

L

L

G

R

Q

M

L

V

G

Q

L

L

G

E

H

T

L

F

G

A

D

Q

R
|
R

F

K

P

P

H

H

Q
|
Q

L

L

S
|
S

G

G

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

V

V

V

Y

N

N

K

P

P

P

R

V

L

I

L

L

L

D

D

E

Q

P

S

L

L

S

S

N

A

L

L

D

D

A

Y

K

K

L

L

R

R

E

K

E

Q

A

M

R

Q

A

N

F

E

L

L

R

K

E

A

L

L

I

Q

V

R

R

K

L

L

G

G

L

I

S

T

A

F

L

V

M

F

V

V

T

T

H

H

D

D

Q

Q

A

E

E

E

A

A

M

L

A

T

I

M

S

S

D

D

R

R

I

I

L

V

L

M

N

R

N

D

G

G

K

R

I

I

E

E

Q

Q

Q

D

G

G

T

T

P

P

Q

R

S

E

M

I

Y

Y

E

E

T

E

P

P

D

K

T

N

L

L

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

E

N

I

N

N

R

M

L

F

P

N

A

A

K

T

L

V

L

I

Q

E

R

R

-

L

D

D

G

E

A

Q

R

R

V

V

L

R

L

A

Q

N

V

V

E

E

G

G

G

R

N

E

V

C

T

N

A

I

T

Y

L

V

R

N

G

F

A

A

A

V

G

E

N

P

D

G

A

Q

K

K

D

L

A

H

I

V

A

L

I

L

-

R

-

P

-

E

-

D

I

L

R

R

V

V

E

E

Q

E

V

I

N

N

binding adenosine-5'-triphosphate: G38 (= G43), K41 (= K46), T42 (= T47), T43 (= T48), R128 (= R139), Q132 (= Q143), S134 (= S145)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 15

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
45% identity, 70% coverage: 1:254/362 of query aligns to 1:247/393 of P9WQI3

query
sites
P9WQI3

M

M

S

A

E

E

L

I

S

V

V

L

N

D

N

H

L

V

H

N

L

K

D

S

Y

Y

G

P

S

D

G

G

A

H

S

T

A

A

N

-

P

-

I

-

L

V

K

R

G

D

V

L

S

N

M

L

E

T

L

I

Q

A

R

D

G

G

E

E

V

F

V

L

A

I

L

L

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

T

L

T

L

L

R

N

A

M

V

I

A

A

G

G

L

L

E

E

S

D

P

I

K

S

S

S

G

G

S

E

I

L

Q

R

I

I

G

A

E

-

R

-

I

-

M

-

F

-

D

-

G

G

Q

E

R

R

K

V

L

N

E

E

I

K

P

A

A

P

E

K

E

D

R

R

N

D

L

I

G

A

L

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

W

Y

P

P

H

H

K

M

T

T

V

V

S

R

D

Q

N

N

V

I

G

A

Y

F

G

P

L

L

K

T

L

L

R

A

K

K

M

M

S

R

S

K

S

A

D

D

I

I

A

A

T

Q

R

K

V

V

K

S

T

E

V

T

L

A

G

K

Q

I

L

L

G

D

L

L

G

T

H

N

L

L

G

L

D

D

R

R

F

K

P

P

H

S

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

L

I

V

V

Y

R

N

H

P

P

P

K

V

A

I

F

L

L

L

M

D

D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

E

V

E

Q

A

M

R

R

A

G

F

E

L

I

R

A

E

Q

L

L

I

Q

V

R

R

R

L

L

G

G

L

T

S

T

A

T

L

V

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

T

E

E

A

A

M

M

A

T

I

L

S

G

D

D

R

R

I

V

L

V

L

M

N

Y

N

G

G

G

K

I

I

A

E

Q

Q

Q

Q

I

G

G

T

T

P

P

Q

E

S

E

M

L

Y

Y

E

E

T

R

P

P

D

A

T

N

L

L

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

A

-

M

N

N

R

F

L

F

P

P

A

A

K

R

L

L

H193 (= H202) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 82% coverage: 1:298/362 of query aligns to 1:284/353 of 1oxvD

query
sites
1oxvD

M

M

S

V

E

R

L

I

S

I

V

V

N

K

N

N

L

V

H

S

L

K

D

V

Y
x
F

G

K

S

K

G

G

A

K

S

V

A

V

N

A

P

-

I

-

L

L

K

D

G

N

V

V

S

N

M

I

E

N

L

I

Q

E

R

N

G

G

E

E

V

R

V

F

A

G

L

I

L

L

G

G

P

P

S
|
S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

A

I

V

I

A

A

G

G

L

L

E

D

S

V

P

P

K

S

S

T

G

G

S

E

I

L

Q

Y

I

F

G

D

E

D

R

R

I

L

M

V

F

-

D

A

G

S

Q

N

R

G

K

K

L

L

E

I

I

V

P

P

A

P

E

E

E

D

R

R

N

K

L

I

G

G

L

M

V

V

F

F

Q
|
Q

S

T

Y

W

A

A

L

L

W

Y

P

P

H

N

K

L

T

T

V

A

S

F

D

E

N

N

V

I

G

A

Y

F

G

P

L

L

K

T

L

N

R

M

K

K

M

M

S

S

S

K

S

E

D

E

I

I

A

R

T

K

R

R

V

V

K

E

T

E

V

V

L

A

G

K

Q

I

L

L

G

D

L

I

G

H

H

H

L

V

G

L

D

N

R

H

F

F

P

P

H

R

Q

E

L

L

S

S

G

G

Q

Q

R

R

V

V

A

A

I

L

A

A

R

R

A

A

L

L

V

V

Y

K

N

D

P

P

P

S

V

L

I

L

L

L

D

D

E
|
E

P

P

L

F

S

S

N

N

L

L

D

D

A

A

K

R

L

M

R

R

E

D

E

S

A

A

R

R

A

A

F

L

L

V

R

K

E

E

L

V

I

Q

V

S

R

R

L

L

G

G

L

V

S

T

A

L

L

L

M

V

V

V

T

S

H

H

D

D

Q

P

A

A

E

D

A

I

M

F

A

A

I

I

S

A

D

D

R

R

I

V

L

G

L

V

L

L

N

V

N

K

G

G

K

K

I

L

E

V

Q

Q

Q

V

G

G

T

K

P

P

Q

E

S

D

M

L

Y

Y

E

D

T

N

P

P

D

V

T

S

L

I

F

Q

T

V

A

A

E

S

F

L

M

I

G

G

S

E

N

I

N

N

R

E

L

L

P

E

A

G

K

K

L

V

L

T

Q

N

R

E

D

-

G

-

A

-

R

-

V

-

L

-

Q

-

V

-

E

-

G

-

G

G

N

V

V

V

T

I

A

G

T

S

L

L

R

R

G

F

A

P

A

V

G

S

N

V

D

S

A

S

K

D

D

R

A

A

I

I

A

I

I

G

I

I

R

R

V

P

E

E

Q

D

V

V

N

K

I

L

S

S

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y13), S40 (= S42), G41 (= G43), G43 (= G45), K44 (= K46), T45 (= T47), T46 (= T48), Q89 (= Q92), E166 (= E169)
binding magnesium ion: T45 (= T47), Q89 (= Q92)

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 82% coverage: 1:298/362 of query aligns to 1:284/353 of 1oxvA

query
sites
1oxvA

M

M

S

V

E

R

L

I

S

I

V

V

N

K

N

N

L

V

H

S

L

K

D

V

Y
x
F

G

K

S

K

G

G

A

K

S

V

A

V

N

A

P

-

I

-

L

L

K

D

G

N

V

V

S

N

M

I

E

N

L

I

Q

E

R

N

G

G

E

E

V

R

V

F

A

G

L

I

L

L

G

G

P

P

S
|
S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

A

I

V

I

A

A

G

G

L

L

E

D

S

V

P

P

K

S

S

T

G

G

S

E

I

L

Q

Y

I

F

G

D

E

D

R

R

I

L

M

V

F

-

D

A

G

S

Q

N

R

G

K

K

L

L

E

I

I

V

P

P

A

P

E

E

E

D

R

R

N

K

L

I

G

G

L

M

V

V

F

F

Q

Q

S

T

Y

W

A

A

L

L

W

Y

P

P

H

N

K

L

T

T

V

A

S

F

D

E

N

N

V

I

G

A

Y

F

G

P

L

L

K

T

L

N

R

M

K

K

M

M

S

S

S

K

S

E

D

E

I

I

A

R

T

K

R

R

V

V

K

E

T

E

V

V

L

A

G

K

Q

I

L

L

G

D

L

I

G

H

H

H

L

V

G

L

D

N

R

H

F

F

P

P

H

R

Q

E

L

L

S

S

G

G

Q

Q

R

R

V

V

A

A

I

L

A

A

R

R

A

A

L

L

V

V

Y

K

N

D

P

P

P

S

V

L

I

L

L

L

D

D

E
|
E

P

P

L

F

S

S

N

N

L

L

D

D

A

A

K

R

L

M

R

R

E

D

E

S

A

A

R

R

A

A

F

L

L

V

R

K

E

E

L

V

I

Q

V

S

R

R

L

L

G

G

L

V

S

T

A

L

L

L

M

V

V

V

T

S

H

H

D

D

Q

P

A

A

E

D

A

I

M

F

A

A

I

I

S

A

D

D

R

R

I

V

L

G

L

V

L

L

N

V

N

K

G

G

K

K

I

L

E

V

Q

Q

Q

V

G

G

T

K

P

P

Q

E

S

D

M

L

Y

Y

E

D

T

N

P

P

D

V

T

S

L

I

F

Q

T

V

A

A

E

S

F

L

M

I

G

G

S

E

N

I

N

N

R

E

L

L

P

E

A

G

K

K

L

V

L

T

Q

N

R

E

D

-

G

-

A

-

R

-

V

-

L

-

Q

-

V

-

E

-

G

-

G

G

N

V

V

V

T

I

A

G

T

S

L

L

R

R

G

F

A

P

A

V

G

S

N

V

D

S

A

S

K

D

D

R

A

A

I

I

A

I

I

G

I

I

R

R

V

P

E

E

Q

D

V

V

N

K

I

L

S

S

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y13), S40 (= S42), G41 (= G43), G43 (= G45), K44 (= K46), T45 (= T47), T46 (= T48), E166 (= E169)

1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 82% coverage: 1:298/362 of query aligns to 1:284/353 of 1oxuA

query
sites
1oxuA

M

M

S

V

E

R

L

I

S

I

V

V

N

K

N

N

L

V

H

S

L

K

D

V

Y
x
F

G

K

S

K

G

G

A

K

S

V

A

V

N
x
A

P

-

I

-

L

L

K

D

G

N

V

V

S

N

M

I

E

N

L

I

Q

E

R

N

G

G

E

E

V

R

V

F

A

G

L

I

L

L

G

G

P

P

S

S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

A

I

V

I

A

A

G

G

L

L

E

D

S

V

P

P

K

S

S

T

G

G

S

E

I

L

Q

Y

I

F

G

D

E

D

R

R

I

L

M

V

F

-

D

A

G

S

Q

N

R

G

K

K

L

L

E

I

I

V

P

P

A

P

E

E

E

D

R

R

N

K

L

I

G

G

L

M

V

V

F

F

Q

Q

S

T

Y

W

A

A

L

L

W

Y

P

P

H

N

K

L

T

T

V

A

S

F

D

E

N

N

V

I

G

A

Y

F

G

P

L

L

K

T

L

N

R

M

K

K

M

M

S

S

S

K

S

E

D

E

I

I

A

R

T

K

R

R

V

V

K

E

T

E

V

V

L

A

G

K

Q

I

L

L

G

D

L

I

G

H

H

H

L

V

G

L

D

N

R

H

F

F

P

P

H

R

Q

E

L

L

S

S

G

G

Q

Q

R

R

V

V

A

A

I

L

A

A

R

R

A

A

L

L

V

V

Y

K

N

D

P

P

P

S

V

L

I

L

L

L

D

D

E

E

P

P

L

F

S

S

N

N

L

L

D

D

A

A

K

R

L

M

R

R

E

D

E

S

A

A

R

R

A

A

F

L

L

V

R

K

E

E

L

V

I

Q

V

S

R

R

L

L

G

G

L

V

S

T

A

L

L

L

M

V

V

V

T

S

H

H

D

D

Q

P

A

A

E

D

A

I

M

F

A

A

I

I

S

A

D

D

R

R

I

V

L

G

L

V

L

L

N

V

N

K

G

G

K

K

I

L

E

V

Q

Q

Q

V

G

G

T

K

P

P

Q

E

S

D

M

L

Y

Y

E

D

T

N

P

P

D

V

T

S

L

I

F

Q

T

V

A

A

E

S

F

L

M

I

G

G

S

E

N

I

N

N

R

E

L

L

P

E

A

G

K

K

L

V

L

T

Q

N

R

E

D

-

G

-

A

-

R

-

V

-

L

-

Q

-

V

-

E

-

G

-

G

G

N

V

V

V

T

I

A

G

T

S

L

L

R

R

G

F

A

P

A

V

G

S

N

V

D

S

A

S

K

D

D

R

A

A

I

I

A

I

I

G

I

I

R

R

V

P

E

E

Q

D

V

V

N

K

I

L

S

S

binding adenosine-5'-diphosphate: F13 (≠ Y13), A20 (≠ N20), G41 (= G43), G43 (= G45), K44 (= K46), T45 (= T47), T46 (= T48)

Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
39% identity, 82% coverage: 1:298/362 of query aligns to 1:284/353 of Q97UY8

query
sites
Q97UY8

M

M

S

V

E

R

L

I

S

I

V

V

N

K

N

N

L

V

H

S

L

K

D

V

Y

F

G

K

S

K

G

G

A

K

S

V

A

V

N

A

P

-

I

-

L

L

K

D

G

N

V

V

S

N

M

I

E

N

L

I

Q

E

R

N

G

G

E

E

V

R

V

F

A

G

L

I

L

L

G

G

P

P

S

S

G

G

S

A

G

G

K

K

T

T

L

F

L

M

R

R

A

I

V

I

A

A

G

G

L

L

E

D

S

V

P

P

K

S

S

T

G

G

S

E

I

L

Q

Y

I

F

G

D

E

D

R

R

I

L

M

V

F

-

D

A

G

S

Q

N

R

G

K

K

L

L

E

I

I

V

P

P

A

P

E

E

E

D

R

R

N

K

L

I

G

G

L

M

V

V

F

F

Q

Q

S

T

Y

W

A

A

L

L

W

Y

P

P

H

N

K

L

T

T

V

A

S

F

D

E

N

N

V

I

G

A

Y

F

G

P

L

L

K

T

L

N

R

M

K

K

M

M

S

S

S

K

S

E

D

E

I

I

A

R

T

K

R

R

V

V

K

E

T

E

V

V

L

A

G

K

Q

I

L

L

G

D

L

I

G

H

H

H

L

V

G

L

D

N

R

H

F

F

P

P

H

R

Q

E

L

L

S
|
S

G

G

G
|
G

Q

Q

R

R

V

V

A

A

I

L

A

A

R

R

A

A

L

L

V

V

Y

K

N

D

P

P

P

S

V

L

I

L

L

L

D

D

E
|
E

P

P

L

F

S

S

N

N

L

L

D

D

A

A

K

R

L

M

R

R

E

D

E

S

A

A

R

R

A

A

F

L

L

V

R

K

E

E

L

V

I

Q

V

S

R

R

L

L

G

G

L

V

S

T

A

L

L

L

M

V

V

V

T

S

H

H

D

D

Q

P

A

A

E

D

A

I

M

F

A

A

I

I

S

A

D

D

R

R

I

V

L

G

L

V

L

L

N

V

N

K

G

G

K

K

I

L

E

V

Q

Q

Q

V

G

G

T

K

P

P

Q

E

S

D

M

L

Y

Y

E

D

T

N

P

P

D

V

T

S

L

I

F

Q

T

V

A

A

E

S

F

L

M

I

G

G

S

E

N

I

N

N

R

E

L

L

P

E

A

G

K

K

L

V

L

T

Q

N

R

E

D

-

G

-

A

-

R

-

V

-

L

-

Q

-

V

-

E

-

G

-

G

G

N

V

V

V

T

I

A

G

T

S

L

L

R

R

G

F

A

P

A

V

G

S

N

V

D

S

A

S

K

D

D

R

A

A

I

I

A

I

I

G

I

I

R

R

V

P

E

E

Q

D

V

V

N

K

I

L

S

S

S142 (= S145) mutation to A: Decrease in ATPase activity. Can form dimers.
G144 (= G147) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
E166 (= E169) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
49% identity, 64% coverage: 24:254/362 of query aligns to 19:245/374 of 2awnB

query
sites
2awnB

K

K

G

D

V

I

S

N

M

L

E

D

L

I

Q

H

R

E

G

G

E

E

V

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

S

T

P

I

K

T

S

S

G

G

S

D

I

L

Q

F

I

I

G

G

E

E

R

K

I

R

M

M

F

N

D

D

G

-

Q

-

R

-

K

-

L

-

E

-

I

T

P

P

A

P

E

A

E

E

R

R

N

G

L

V

G

G

L

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

W

Y

P

P

H

H

K

L

T

S

V

V

S

A

D

E

N

N

V

M

G

S

Y

F

G

G

L

L

K

K

L

L

R

A

K

G

M

A

S

K

S

E

D

V

I

I

A

N

T

Q

R

R

V

V

K

N

T

Q

V

V

L

A

G

E

Q

V

L

L

G

Q

L

L

G

A

H

H

L

L

G

L

D

D

R

R

F

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

V

V

Y

A

N

E

P

P

P

S

V

V

I

F

L

L

D

D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

A

M

R

R

A

I

F

E

L

I

R

S

E

R

L

L

I

H

V

K

R

R

L

L

G

G

L

R

S

T

A

M

L

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

A

V

E

E

A

A

M

M

A

T

I

L

S

A

D

D

R

K

I

I

L

V

L

L

N

D

N

A

G

G

K

R

I

V

E

A

Q

Q

Q

V

G

G

T

K

P

P

Q

L

S

E

M

L

Y

Y

E

H

T

Y

P

P

D

A

T

D

L

R

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

K

-

M

N

N

R

F

L

L

P

P

A

V

K

K

L

V

binding adenosine-5'-diphosphate: S37 (= S42), G38 (= G43), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 17

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
49% identity, 64% coverage: 24:254/362 of query aligns to 19:245/371 of 3puyA

query
sites
3puyA

K

K

G

D

V

I

S

N

M

L

E

D

L

I

Q

H

R

E

G

G

E

E

V

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

S

T

P

I

K

T

S

S

G

G

S

D

I

L

Q

F

I

I

G

G

E

E

R

K

I

R

M

M

F

N

D

D

G

-

Q

-

R

-

K

-

L

-

E

-

I

T

P

P

A

P

E

A

E

E

R

R

N

G

L

V

G

G

L

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

W

Y

P

P

H

H

K

L

T

S

V

V

S

A

D

E

N

N

V

M

G

S

Y

F

G

G

L

L

K

K

L

L

R

A

K

G

M

A

S

K

S

E

D

V

I

I

A

N

T

Q

R

R

V

V

K

N

T

Q

V

V

L

A

G

E

Q

V

L

L

G

Q

L

L

G

A

H

H

L

L

G

L

D

D

R
|
R

F

K

P

P

H

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

V

V

Y

A

N

E

P

P

P

S

V

V

I

F

L

L

D

D

E
|
E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

A

M

R

R

A

I

F

E

L

I

R

S

E

R

L

L

I

H

V

K

R

R

L

L

G

G

L

R

S

T

A

M

L

I

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

V

E

E

A

A

M

M

A

T

I

L

S

A

D

D

R

K

I

I

L

V

L

L

N

D

N

A

G

G

K

R

I

V

E

A

Q

Q

Q

V

G

G

T

K

P

P

Q

L

S

E

M

L

Y

Y

E

H

T

Y

P

P

D

A

T

D

L

R

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

K

-

M

N

N

R

F

L

L

P

P

A

V

K

K

L

V

binding phosphoaminophosphonic acid-adenylate ester: S37 (= S42), G38 (= G43), C39 (≠ S44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), Q81 (= Q92), R128 (= R139), A132 (≠ Q143), S134 (= S145), G136 (= G147), Q137 (= Q148), E158 (= E169), H191 (= H202)
binding magnesium ion: S42 (≠ T47), Q81 (= Q92)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 12

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
49% identity, 64% coverage: 24:254/362 of query aligns to 19:245/371 of 3puxA

query
sites
3puxA

K

K

G

D

V

I

S

N

M

L

E

D

L

I

Q

H

R

E

G

G

E

E

V

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

S

T

P

I

K

T

S

S

G

G

S

D

I

L

Q

F

I

I

G

G

E

E

R

K

I

R

M

M

F

N

D

D

G

-

Q

-

R

-

K

-

L

-

E

-

I

T

P

P

A

P

E

A

E

E

R

R

N

G

L

V

G

G

L

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

W

Y

P

P

H

H

K

L

T

S

V

V

S

A

D

E

N

N

V

M

G

S

Y

F

G

G

L

L

K

K

L

L

R

A

K

G

M

A

S

K

S

E

D

V

I

I

A

N

T

Q

R

R

V

V

K

N

T

Q

V

V

L

A

G

E

Q

V

L

L

G

Q

L

L

G

A

H

H

L

L

G

L

D

D

R
|
R

F

K

P

P

H

K

Q

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

V

V

Y

A

N

E

P

P

P

S

V

V

I

F

L

L

D

D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

A

M

R

R

A

I

F

E

L

I

R

S

E

R

L

L

I

H

V

K

R

R

L

L

G

G

L

R

S

T

A

M

L

I

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

V

E

E

A

A

M

M

A

T

I

L

S

A

D

D

R

K

I

I

L

V

L

L

N

D

N

A

G

G

K

R

I

V

E

A

Q

Q

Q

V

G

G

T

K

P

P

Q

L

S

E

M

L

Y

Y

E

H

T

Y

P

P

D

A

T

D

L

R

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

K

-

M

N

N

R

F

L

L

P

P

A

V

K

K

L

V

binding adenosine-5'-diphosphate: G38 (= G43), C39 (≠ S44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), R128 (= R139), S134 (= S145), Q137 (= Q148)
binding beryllium trifluoride ion: S37 (= S42), G38 (= G43), K41 (= K46), Q81 (= Q92), S134 (= S145), G136 (= G147), H191 (= H202)
binding magnesium ion: S42 (≠ T47), Q81 (= Q92)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
49% identity, 64% coverage: 24:254/362 of query aligns to 19:245/371 of 3puwA

query
sites
3puwA

K

K

G

D

V

I

S

N

M

L

E

D

L

I

Q

H

R

E

G

G

E

E

V

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

S

T

P

I

K

T

S

S

G

G

S

D

I

L

Q

F

I

I

G

G

E

E

R

K

I

R

M

M

F

N

D

D

G

-

Q

-

R

-

K

-

L

-

E

-

I

T

P

P

A

P

E

A

E

E

R

R

N

G

L

V

G

G

L

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

W

Y

P

P

H

H

K

L

T

S

V

V

S

A

D

E

N

N

V

M

G

S

Y

F

G

G

L

L

K

K

L

L

R

A

K

G

M

A

S

K

S

E

D

V

I

I

A

N

T

Q

R

R

V

V

K

N

T

Q

V

V

L

A

G

E

Q

V

L

L

G

Q

L

L

G

A

H

H

L

L

G

L

D

D

R
|
R

F

K

P

P

H

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

V

V

Y

A

N

E

P

P

P

S

V

V

I

F

L

L

D

D

E
|
E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

A

M

R

R

A

I

F

E

L

I

R

S

E

R

L

L

I

H

V

K

R

R

L

L

G

G

L

R

S

T

A

M

L

I

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

V

E

E

A

A

M

M

A

T

I

L

S

A

D

D

R

K

I

I

L

V

L

L

N

D

N

A

G

G

K

R

I

V

E

A

Q

Q

Q

V

G

G

T

K

P

P

Q

L

S

E

M

L

Y

Y

E

H

T

Y

P

P

D

A

T

D

L

R

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

K

-

M

N

N

R

F

L

L

P

P

A

V

K

K

L

V

binding adenosine-5'-diphosphate: G38 (= G43), C39 (≠ S44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), R128 (= R139), A132 (≠ Q143), S134 (= S145), Q137 (= Q148)
binding tetrafluoroaluminate ion: S37 (= S42), G38 (= G43), K41 (= K46), Q81 (= Q92), S134 (= S145), G135 (= G146), G136 (= G147), E158 (= E169), H191 (= H202)
binding magnesium ion: S42 (≠ T47), Q81 (= Q92)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 17

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
49% identity, 64% coverage: 24:254/362 of query aligns to 19:245/371 of 3puvA

query
sites
3puvA

K

K

G

D

V

I

S

N

M

L

E

D

L

I

Q

H

R

E

G

G

E

E

V

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

S

T

P

I

K

T

S

S

G

G

S

D

I

L

Q

F

I

I

G

G

E

E

R

K

I

R

M

M

F

N

D

D

G

-

Q

-

R

-

K

-

L

-

E

-

I

T

P

P

A

P

E

A

E

E

R

R

N

G

L

V

G

G

L

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

W

Y

P

P

H

H

K

L

T

S

V

V

S

A

D

E

N

N

V

M

G

S

Y

F

G

G

L

L

K

K

L

L

R

A

K

G

M

A

S

K

S

E

D

V

I

I

A

N

T

Q

R

R

V

V

K

N

T

Q

V

V

L

A

G

E

Q

V

L

L

G

Q

L

L

G

A

H

H

L

L

G

L

D

D

R
|
R

F

K

P

P

H

K

Q
x
A

L

L

S
|
S

G

G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

V

V

Y

A

N

E

P

P

P

S

V

V

I

F

L

L

D

D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

A

M

R

R

A

I

F

E

L

I

R

S

E

R

L

L

I

H

V

K

R

R

L

L

G

G

L

R

S

T

A

M

L

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

A

V

E

E

A

A

M

M

A

T

I

L

S

A

D

D

R

K

I

I

L

V

L

L

N

D

N

A

G

G

K

R

I

V

E

A

Q

Q

Q

V

G

G

T

K

P

P

Q

L

S

E

M

L

Y

Y

E

H

T

Y

P

P

D

A

T

D

L

R

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

K

-

M

N

N

R

F

L

L

P

P

A

V

K

K

L

V

binding adenosine-5'-diphosphate: G38 (= G43), C39 (≠ S44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), R128 (= R139), A132 (≠ Q143), S134 (= S145), Q137 (= Q148)
binding magnesium ion: S42 (≠ T47), Q81 (= Q92)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 17

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
49% identity, 64% coverage: 24:254/362 of query aligns to 20:246/371 of P68187

query
sites
P68187

K

K

G

D

V

I

S

N

M

L

E

D

L

I

Q

H

R

E

G

G

E

E

V

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

S

T

P

I

K

T

S

S

G

G

S

D

I

L

Q

F

I

I

G

G

E

E

R

K

I

R

M

M

F

N

D

D

G

-

Q

-

R

-

K

-

L

-

E

-

I

T

P

P

A

P

E

A

E

E

R

R

N

G

L

V

G

G

L

M

V

V

F

F

Q

Q

S

S

Y

Y

A
|
A

L

L

W

Y

P

P

H

H

K

L

T

S

V

V

S

A

D

E

N

N

V

M

G

S

Y

F

G

G

L

L

K

K

L

L

R

A

K

G

M

A

S
x
K

S

K

S

E

D

V

I

I

A

N

T

Q

R

R

V
|
V

K

N

T

Q

V
|
V

L

A

G
x
E

Q

V

L

L

G

Q

L

L

G
x
A

H

H

L

L

G

L

D

D

R

R

F

K

P

P

H

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

V

V

Y

A

N

E

P

P

P

S

V

V

I

F

L

L

D
|
D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

A

M

R

R

A

I

F

E

L

I

R

S

E

R

L

L

I

H

V

K

R

R

L

L

G

G

L

R

S

T

A

M

L

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

A

V

E

E

A

A

M

M

A

T

I

L

S

A

D

D

R

K

I

I

L

V

L

L

N

D

N

A

G

G

K

R

I

V

E

A

Q

Q

Q

V

G

G

T

K

P

P

Q

L

S

E

M

L

Y

Y

E

H

T

Y

P

P

D

A

T

D

L
x
R

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

K

-

M

N

N

R
x
F

L

L

P

P

A

V

K

K

L

V

A85 (= A95) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ S116) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V124) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (= V127) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ G129) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ G134) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G147) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D168) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ L238) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (≠ R249) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
49% identity, 64% coverage: 24:254/362 of query aligns to 17:243/367 of 1q12A

query
sites
1q12A

K

K

G

D

V

I

S

N

M

L

E

D

L

I

Q

H

R

E

G

G

E

E

V

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

S

T

P

I

K

T

S

S

G

G

S

D

I

L

Q

F

I

I

G

G

E

E

R

K

I

R

M

M

F

N

D

D

G

-

Q

-

R

-

K

-

L

-

E

-

I

T

P

P

A

P

E

A

E

E

R

R

N

G

L

V

G

G

L

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

W

Y

P

P

H

H

K

L

T

S

V

V

S

A

D

E

N

N

V

M

G

S

Y

F

G

G

L

L

K

K

L

L

R

A

K

G

M

A

S

K

S

E

D

V

I

I

A

N

T

Q

R

R

V

V

K

N

T

Q

V

V

L

A

G

E

Q

V

L

L

G

Q

L

L

G

A

H

H

L

L

G

L

D

D

R
|
R

F

K

P

P

H

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

V

V

Y

A

N

E

P

P

P

S

V

V

I

F

L

L

D

D

E

E

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

A

M

R

R

A

I

F

E

L

I

R

S

E

R

L

L

I

H

V

K

R

R

L

L

G

G

L

R

S

T

A

M

L

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

A

V

E

E

A

A

M

M

A

T

I

L

S

A

D

D

R

K

I

I

L

V

L

L

N

D

N

A

G

G

K

R

I

V

E

A

Q

Q

Q

V

G

G

T

K

P

P

Q

L

S

E

M

L

Y

Y

E

H

T

Y

P

P

D

A

T

D

L

R

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

K

-

M

N

N

R

F

L

L

P

P

A

V

K

K

L

V

binding adenosine-5'-triphosphate: S35 (= S42), G36 (= G43), C37 (≠ S44), G38 (= G45), K39 (= K46), S40 (≠ T47), T41 (= T48), R126 (= R139), A130 (≠ Q143), S132 (= S145), G134 (= G147), Q135 (= Q148)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 10

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
48% identity, 64% coverage: 24:254/362 of query aligns to 20:246/369 of P19566

query
sites
P19566

K

K

G

D

V

I

S

N

M

L

E

D

L

I

Q

H

R

D

G

G

E

E

V

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

L

L

R

R

A

M

V

I

A

A

G

G

L

L

E

E

S

T

P

I

K

T

S

S

G

G

S

D

I

L

Q

F

I

I

G

G

E

E

R

T

I

R

M

M

F

N

D

D

G

-

Q

-

R

-

K

-

L

-

E

-

I

I

P

P

A

P

E

A

E

E

R

R

N

G

L

V

G

G

L

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L
|
L

W

Y

P

P

H

H

K

L

T

S

V

V

S

A

D

E

N

N

V

M

G

S

Y

F

G

G

L

L

K

K

L

L

R

A

K

G

M

A

S

K

S

E

D

V

I

M

A

N

T

Q

R

R

V

V

K

N

T

Q

V

V

L

A

G

E

Q

V

L

L

G

Q

L

L

G

A

H

H

L

L

G

L

D

E

R

R

F

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

V

V

Y

A

N

E

P

P

P

R

V

V

I

F

L

L

D

D

E

E

P
|
P

L

L

S

S

N

N

L

L

D
|
D

A

A

K

A

L

L

R

R

E

V

E

Q

A

M

R

R

A

I

F

E

L

I

R

S

E

R

L

L

I

H

V

K

R

R

L

L

G

G

L

R

S

T

A

M

L

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

A

V

E

E

A

A

M

M

A

T

I

L

S

A

D

D

R

K

I

I

L

V

L

L

N

D

N

A

G

G

K

R

I

V

E

A

Q

Q

Q

V

G

G

T

K

P

P

Q

L

S

E

M

L

Y

Y

E

H

T

Y

P

P

D

A

T

D

L

R

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

K

-

M

N

N

R

F

L

L

P

P

A

V

K

K

L

V

L86 (= L96) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P170) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D175) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

8hplC Lpqy-sugabc in state 1 (see paper)
45% identity, 67% coverage: 11:252/362 of query aligns to 5:242/384 of 8hplC

query
sites
8hplC

L

L

D

D

Y

R

G

V

S

T

G

K

A

S

S
x
Y

A

P

N

R

P

A

I

A

L

V

K

K

G

E

V

F

S

S

M

M

E

T

L

I

Q

A

R

D

G

G

E

E

V

F

V

I

A

I

L

L

L

V

G

G

P

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

A

M

V

I

A

A

G

G

L

L

E

E

S

E

P

I

K

T

S

S

G

G

S

E

I

L

Q

R

I

I

G

G

E

-

R

-

I

-

M

-

F

-

D

-

G

G

Q

E

R

R

K

V

L

N

E

E

I

K

P

A

A

P

E

K

E

D

R

R

N

D

L

I

G

A

L

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

W

Y

P

P

H

H

K

M

T

T

V

V

S

R

D

Q

N

N

V

I

G

A

Y

F

G

P

L

L

K

T

L

L

R

A

K

K

M

V

S

P

S

K

S

A

D

E

I

I

A

A

T

A

R

K

V

V

K

E

T

E

V

T

L

A

G

K

Q

I

L

L

G

D

L

L

G

S

H

E

L

L

G

L

D

D

R

R

F

K

P

P

H

G

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

L

I

V

V

Y

R

N

S

P

P

P

K

V

A

I

F

L

L

L

M

D

D

E

Q

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

E

V

E

Q

A

M

R

R

A

A

F

E

L

I

R

S

E

R

L

L

I

Q

V

D

R

R

L

L

G

G

L

T

S

T

A

T

L

V

M

Y

V

V

T

T

H

H

D

D

Q

Q

A

T

E

E

A

A

M

M

A

T

I

L

S

G

D

D

R

R

I

V

L

V

L

M

N

L

N

A

G

G

K

E

I

V

E

Q

Q

Q

Q

I

G

G

T

T

P

P

Q

D

S

E

M

L

Y

Y

E

S

T

S

P

P

D

A

T

N

L

L

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

A

-

M

N

N

R

F

L

F

P

P

A

A

binding adenosine-5'-triphosphate: Y12 (≠ S18), G37 (= G43), C38 (≠ S44), G39 (= G45), K40 (= K46), S41 (≠ T47), T42 (= T48)

8hprD Lpqy-sugabc in state 4 (see paper)
47% identity, 64% coverage: 23:252/362 of query aligns to 19:244/362 of 8hprD

query
sites
8hprD

L

V

K

K

G

E

V

F

S

S

M

M

E

T

L

I

Q

A

R

D

G

G

E

E

V

F

V

I

A

I

L

L

L

V

G

G

P

P

S
|
S

G

G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

A

M

V

I

A

A

G

G

L

L

E

E

S

E

P

I

K

T

S

S

G

G

S

E

I

L

Q

R

I

I

G

G

E

-

R

-

I

-

M

-

F

-

D

-

G

G

Q

E

R

R

K

V

L

N

E

E

I

K

P

A

A

P

E

K

E

D

R

R

N

D

L

I

G

A

L

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

W

Y

P

P

H

H

K

M

T

T

V

V

S

R

D

Q

N

N

V

I

G

A

Y

F

G

P

L

L

K

T

L

L

R

A

K

K

M

V

S

P

S

K

S

A

D

E

I

I

A

A

T

A

R

K

V

V

K

E

T

E

V

T

L

A

G

K

Q

I

L

L

G

D

L

L

G

S

H

E

L

L

G

L

D

D

R
|
R

F

K

P

P

H

G

Q
|
Q

L

L

S
|
S

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

L

I

V

V

Y

R

N

S

P

P

P

K

V

A

I

F

L

L

L

M

D

D

E
x
Q

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

E

V

E

Q

A

M

R

R

A

A

F

E

L

I

R

S

E

R

L

L

I

Q

V

D

R

R

L

L

G

G

L

T

S

T

A

T

L

V

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

T

E

E

A

A

M

M

A

T

I

L

S

G

D

D

R

R

I

V

L

V

L

M

N

L

N

A

G

G

K

E

I

V

E

Q

Q

Q

Q

I

G

G

T

T

P

P

Q

D

S

E

M

L

Y

Y

E

S

T

S

P

P

D

A

T

N

L

L

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

A

-

M

N

N

R

F

L

F

P

P

A

A

binding adenosine-5'-triphosphate: S38 (= S42), C40 (≠ S44), G41 (= G45), K42 (= K46), S43 (≠ T47), T44 (= T48), Q82 (= Q92), R129 (= R139), Q133 (= Q143), S135 (= S145), G136 (= G146), G137 (= G147), Q159 (≠ E169), H192 (= H202)
binding magnesium ion: S43 (≠ T47), Q82 (= Q92)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprC Lpqy-sugabc in state 4 (see paper)
47% identity, 64% coverage: 23:252/362 of query aligns to 19:244/363 of 8hprC

query
sites
8hprC

L

V

K

K

G

E

V

F

S

S

M

M

E

T

L

I

Q

A

R

D

G

G

E

E

V

F

V

I

A

I

L

L

L

V

G

G

P

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
x
S

T

T

L

T

L

L

R

N

A

M

V

I

A

A

G

G

L

L

E

E

S

E

P

I

K

T

S

S

G

G

S

E

I

L

Q

R

I

I

G

G

E

-

R

-

I

-

M

-

F

-

D

-

G

G

Q

E

R

R

K

V

L

N

E

E

I

K

P

A

A

P

E

K

E

D

R

R

N

D

L

I

G

A

L

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

W

Y

P

P

H

H

K

M

T

T

V

V

S

R

D

Q

N

N

V

I

G

A

Y

F

G

P

L

L

K

T

L

L

R

A

K

K

M

V

S

P

S

K

S

A

D

E

I

I

A

A

T

A

R

K

V

V

K

E

T

E

V

T

L

A

G

K

Q

I

L

L

G

D

L

L

G

S

H

E

L

L

G

L

D

D

R

R

F

K

P

P

H

G

Q
|
Q

L

L

S

S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

L

I

V

V

Y

R

N

S

P

P

P

K

V

A

I

F

L

L

L

M

D

D

E

Q

P

P

L

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

E

V

E

Q

A

M

R

R

A

A

F

E

L

I

R

S

E

R

L

L

I

Q

V

D

R

R

L

L

G

G

L

T

S

T

A

T

L

V

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

A

T

E

E

A

A

M

M

A

T

I

L

S

G

D

D

R

R

I

V

L

V

L

M

N

L

N

A

G

G

K

E

I

V

E

Q

Q

Q

Q

I

G

G

T

T

P

P

Q

D

S

E

M

L

Y

Y

E

S

T

S

P

P

D

A

T

N

L

L

F

F

T

V

A

A

E

G

F

F

M

I

G

G

S

S

N

P

-

A

-

M

N

N

R

F

L

F

P

P

A

A

binding adenosine-5'-triphosphate: S38 (= S42), G39 (= G43), G41 (= G45), K42 (= K46), S43 (≠ T47), Q82 (= Q92), Q133 (= Q143), G136 (= G146), G137 (= G147), Q138 (= Q148), H192 (= H202)
binding magnesium ion: S43 (≠ T47), Q82 (= Q92)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

Query Sequence

>WP_061945612.1 NCBI__GCF_001584185.1:WP_061945612.1
MSELSVNNLHLDYGSGASANPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPK
SGSIQIGERIMFDGQRKLEIPAEERNLGLVFQSYALWPHKTVSDNVGYGLKLRKMSSSDI
ATRVKTVLGQLGLGHLGDRFPHQLSGGQQQRVAIARALVYNPPVILLDEPLSNLDAKLRE
EARAFLRELIVRLGLSALMVTHDQAEAMAISDRILLLNNGKIEQQGTPQSMYETPDTLFT
AEFMGSNNRLPAKLLQRDGARVLLQVEGGNVTATLRGAAGNDAKDAIAIIRVEQVNISDV
PVENSIKLPLATCMYLGDRWECVFRNSTESGADSSVLRAYARQRLAPGEYWLQLPQDALW
AF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory