SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_062058929.1 NCBI__GCF_001401755.1:WP_062058929.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4l0oH Structure determination of cystathionine gamma-synthase from helicobacter pylori
46% identity, 95% coverage: 16:382/386 of query aligns to 10:373/373 of 4l0oH

query
sites
4l0oH

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active site: R40 (= R48), Y92 (= Y102), D164 (= D174), K189 (= K199)
binding pyridoxal-5'-phosphate: Y38 (= Y46), R40 (= R48), S67 (= S76), G68 (= G77), L69 (≠ M78), Y92 (= Y102), D164 (= D174), S186 (= S196), T188 (= T198), K189 (= K199)

3mkjA Methionine gamma-lyase from citrobacter freundii with pyridoximine-5'- phosphate (see paper)
41% identity, 98% coverage: 2:381/386 of query aligns to 2:383/386 of 3mkjA

query
sites
3mkjA

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active site: Y101 (= Y102), D173 (= D174), K198 (= K199)
binding [5-hydroxy-4-(iminomethyl)-6-methyl-pyridin-3-yl]methyl dihydrogen phosphate: G76 (= G77), I77 (≠ M78), Y101 (= Y102), E144 (= E145), D173 (= D174), F176 (= F177), S195 (= S196), T197 (= T198), K198 (= K199)

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
40% identity, 98% coverage: 2:381/386 of query aligns to 2:394/396 of 4omaA

query
sites
4omaA

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active site: R59 (= R48), Y112 (= Y102), D184 (= D174), K209 (= K199)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G77), I88 (≠ M78), Y112 (= Y102), D184 (= D174), S206 (= S196), T208 (= T198), K209 (= K199), V337 (≠ M324), S338 (= S325), R373 (= R360)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
40% identity, 98% coverage: 2:381/386 of query aligns to 2:394/396 of 3jwbA

query
sites
3jwbA

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L

E

E

K

R

G

G

E

E

S

A

S

G

L

L

V

A

F

T

G

A

S

S

G

G

M

I

A

S

A

A

I

I

S

T

T

T

T

T

L

L

F

L

A

T

F

L

L

C

Q

Q

K

Q

G

G

D

D

H

H

I

I

I

V

L

S

Q

A

R

S

A

A

I

I

Y
|
Y

G

G

G

C

T

T

A

H

N

A

L

F

I

L

N

S

E

H

E

S

F

M

K

P

K

K

H

F

G

G

I

I

D

N

F

V

S

S

Y

F

V

V

D

D

S

A

L

A

S

K

I

P

E

E

D

E

F

I

E

R

N

A

K

A

I

M

Q

R

S

P

N

E

T

T

K

K

I

V

I

V

Y

Y

V

I

E

E

T

T

P

P

S

A

N

N

P

P

L

T

L

L

S

S

I

L

I

V

D

D

L

I

N

E

A

T

I

V

G

A

A

G

L

I

G

A

K

H

K

Q

N

Q

G

G

I

A

I

L

T

L

I

V

V

V

D
|
D

N

N

T

T

F

F

A

M

S

S

P

P

V

Y

N

C

Q

Q

N

Q

P

P

I

L

V

Q

L

L

G

G

I

A

D

D

I

I

V

V

V

V

H

H

S

S

A

V

T

T

K
|
K

Y

Y

L

I

G

N

G

G

H

H

S

G

D

D

I

V

L

I

A

G

G

G

A

I

V

I

I

V

A

G

S

K

E

Q

K

E

N

F

V

I

N

D

Q

Q

I

A

-

R

F

F

E

V

V

G

A

L

K

K

N

D

F

I

-

T

G

G

G

G

N

C

L

M

S

S

D

P

F

F

T

N

V

A

W

W

M

L

L

T

E

L

R

R

S

G

I

V

K

K

T

T

L

L

G

G

I

I

R

R

V

M

E

E

R

R

Q

H

N

C

K

E

N

N

A

A

L

L

K

K

I

I

S

A

K

R

W

F

L

L

Q

E

K

G

N

H

E

P

D

S

I

I

S

T

T

R

V

V

Y

Y

Y

Y

P

P

G

G

L

L

K

S

S

S

H

H

P

P

N

Q

H

Y

E

E

L

L

A

G

K

Q

Q

R

Q

Q

M

M

K

S

G

L

Y

P

G

G

G

G

M

I

L

I

S

S

F

F

E

E

L

I

K

A

E

G

G

G

L

L

S

E

A

A

D

G

L

R

F

R

Q

M

K

I

N

N

-

S

L

V

K

E

L

L

I

C

K

L

S

L

S

A

M

V

S
|
S

L

L

A

G

G

D

V

T

E

E

S

T

T

L

I

I

L

Q

S

H

P

P

H

A

L

S

T

M

S

T

H

H

A

S

L

P

L

V

K

A

K

P

E

E

E

E

R

R

S

L

S

K

Q

A

G

G

I

I

K

T

D

D

E

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

E

D

K

P

E

E

D

D

I

I

I

I

H

N

D

D

I

L

L

E

Q

H

A

A

I

I

E

R

K

K

active site: R59 (= R48), Y112 (= Y102), D184 (= D174), K209 (= K199)
binding norleucine: Y112 (= Y102), K209 (= K199), S338 (= S325), R373 (= R360)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
40% identity, 98% coverage: 2:381/386 of query aligns to 2:394/396 of 3jwaA

query
sites
3jwaA

N

D

T

C

K

R

N

T

I

Y

G

G

F

F

N

N

T

T

I

Q

C

I

T

V

H

H

F

A

G

G

E

Q

I

Q

K

P

D

D

Q

P

Q

S

F

T

K

G

G

A

A

L

I

S

S

T

P

P

I

I

Y

F

L

Q

S

T

T

S

S

T

Y

F

A

V

F

F

E

D

D

S

V

A

E

E

T

Q

K

G

R

A

-

A

-

R

-

F

-

A

-

L

-

E

-

E

-

S

-

G

-

Y

-

I

Y

Y

P

T

R
|
R

Y

L

F

G

N

N

T

-

P

P

N

T

Q

T

E

D

A

A

L

L

C

E

K

K

K

K

I

L

A

A

K

V

L

L

E

E

K

R

G

G

E

E

S

A

S

G

L

L

V

A

F

T

G

A

S

S

G

G

M

I

A

S

A

A

I

I

S

T

T

T

T

T

L

L

F

L

A

T

F

L

L

C

Q

Q

K

Q

G

G

D

D

H

H

I

I

I

V

L

S

Q

A

R

S

A

A

I

I

Y
|
Y

G

G

G

C

T

T

A

H

N

A

L

F

I

L

N

S

E

H

E

S

F

M

K

P

K

K

H

F

G

G

I

I

D

N

F

V

S

S

Y

F

V

V

D

D

S

A

L

A

S

K

I

P

E

E

D

E

F

I

E

R

N

A

K

A

I

M

Q

R

S

P

N

E

T

T

K

K

I

V

I

V

Y

Y

V

I

E

E

T

T

P

P

S

A

N

N

P

P

L

T

L

L

S

S

I

L

I

V

D

D

L

I

N

E

A

T

I

V

G

A

A

G

L

I

G

A

K

H

K

Q

N

Q

G

G

I

A

I

L

T

L

I

V

V

V

D
|
D

N

N

T

T

F

F

A

M

S

S

P

P

V

Y

N

C

Q

Q

N

Q

P

P

I

L

V

Q

L

L

G

G

I

A

D

D

I

I

V

V

V

V

H

H

S

S

A

V

T

T

K
|
K

Y

Y

L

I

G

N

G

G

H

H

S

G

D

D

I

V

L

I

A

G

G

G

A

I

V

I

I

V

A

G

S

K

E

Q

K

E

N

F

V

I

N

D

Q

Q

I

A

-

R

F

F

E

V

V

G

A

L

K

K

N

D

F

I

-

T

G

G

G

G

N

C

L

M

S

S

D

P

F

F

T

N

V

A

W

W

M

L

L

T

E

L

R

R

S

G

I

V

K

K

T

T

L

L

G

G

I

I

R

R

V

M

E

E

R

R

Q

H

N

C

K

E

N

N

A

A

L

L

K

K

I

I

S

A

K

R

W

F

L

L

Q

E

K

G

N

H

E

P

D

S

I

I

S

T

T

R

V

V

Y

Y

Y

Y

P

P

G

G

L

L

K

S

S

S

H

H

P

P

N

Q

H

Y

E

E

L

L

A

G

K

Q

Q

R

Q

Q

M

M

K

S

G

L

Y

P

G

G

G

G

M

I

L

I

S

S

F

F

E

E

L

I

K

A

E

G

G

G

L

L

S

E

A

A

D

G

L

R

F

R

Q

M

K

I

N

N

-

S

L

V

K

E

L

L

I

C

K

L

S

L

S

A

M
x
V

S
|
S

L

L

A

G

G

D

V

T

E

E

S

T

T

L

I

I

L

Q

S

H

P

P

H

A

L

S

T

M

S

T

H

H

A

S

L

P

L

V

K

A

K

P

E

E

E

E

R

R

S

L

S

K

Q

A

G

G

I

I

K

T

D

D

E

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

E

D

K

P

E

E

D

D

I

I

I

I

H

N

D

D

I

L

L

E

Q

H

A

A

I

I

E

R

K

K

active site: R59 (= R48), Y112 (= Y102), D184 (= D174), K209 (= K199)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (= Y102), K209 (= K199), V337 (≠ M324), S338 (= S325), R373 (= R360)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
40% identity, 98% coverage: 2:381/386 of query aligns to 2:394/396 of 3jw9A

query
sites
3jw9A

N

D

T

C

K

R

N

T

I

Y

G

G

F

F

N

N

T

T

I

Q

C

I

T

V

H

H

F

A

G

G

E

Q

I

Q

K

P

D

D

Q

P

Q

S

F

T

K

G

G

A

A

L

I

S

S

T

P

P

I

I

Y

F

L

Q

S

T

T

S

S

T

Y

F

A

V

F

F

E

D

D

S

V

A

E

E

T

Q

K

G

R

A

-

A

-

R

-

F

-

A

-

L

-

E

-

E

-

S

-

G

-

Y

-

I

Y

Y

P

T

R
|
R

Y

L

F

G

N

N

T

-

P

P

N

T

Q

T

E

D

A

A

L

L

C

E

K

K

K

K

I

L

A

A

K

V

L

L

E

E

K

R

G

G

E

E

S

A

S

G

L

L

V

A

F

T

G

A

S

S

G

G

M

I

A

S

A

A

I

I

S

T

T

T

T

T

L

L

F

L

A

T

F

L

L

C

Q

Q

K

Q

G

G

D

D

H

H

I

I

I

V

L

S

Q

A

R

S

A

A

I

I

Y
|
Y

G

G

G

C

T

T

A

H

N

A

L

F

I

L

N

S

E

H

E

S

F

M

K

P

K

K

H

F

G

G

I

I

D

N

F

V

S

S

Y

F

V

V

D

D

S

A

L

A

S

K

I

P

E

E

D

E

F

I

E

R

N

A

K

A

I

M

Q

R

S

P

N

E

T

T

K

K

I

V

I

V

Y

Y

V

I

E

E

T

T

P

P

S

A

N

N

P

P

L

T

L

L

S

S

I

L

I

V

D

D

L

I

N

E

A

T

I

V

G

A

A

G

L

I

G

A

K

H

K

Q

N

Q

G

G

I

A

I

L

T

L

I

V

V

V

D
|
D

N

N

T

T

F

F

A

M

S

S

P

P

V

Y

N

C

Q

Q

N

Q

P

P

I

L

V

Q

L

L

G

G

I

A

D

D

I

I

V

V

V

V

H

H

S

S

A

V

T

T

K
|
K

Y

Y

L

I

G

N

G

G

H

H

S

G

D

D

I

V

L

I

A

G

G

G

A

I

V

I

I

V

A

G

S

K

E

Q

K

E

N

F

V

I

N

D

Q

Q

I

A

-

R

F

F

E

V

V

G

A

L

K

K

N

D

F

I

-

T

G

G

G

G

N

C

L

M

S

S

D

P

F

F

T

N

V

A

W

W

M

L

L

T

E

L

R

R

S

G

I

V

K

K

T

T

L

L

G

G

I

I

R

R

V

M

E

E

R

R

Q

H

N

C

K

E

N

N

A

A

L

L

K

K

I

I

S

A

K

R

W

F

L

L

Q

E

K

G

N

H

E

P

D

S

I

I

S

T

T

R

V

V

Y

Y

Y

Y

P

P

G

G

L

L

K

S

S

S

H

H

P

P

N

Q

H

Y

E

E

L

L

A

G

K

Q

Q

R

Q

Q

M

M

K

S

G

L

Y

P

G

G

G

G

M

I

L

I

S

S

F

F

E

E

L

I

K

A

E

G

G

G

L

L

S

E

A

A

D

G

L

R

F

R

Q

M

K

I

N

N

-

S

L

V

K

E

L

L

I

C

K

L

S

L

S

A

M
x
V

S
|
S

L

L

A

G

G

D

V

T

E

E

S

T

T

L

I

I

L

Q

S

H

P

P

H

A

L

S

T

M

S

T

H

H

A

S

L

P

L

V

K

A

K

P

E

E

E

E

R

R

S

L

S

K

Q

A

G

G

I

I

K

T

D

D

E

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

I

L

E

E

E

D

K

P

E

E

D

D

I

I

I

I

H

N

D

D

I

L

L

E

Q

H

A

A

I

I

E

R

K

K

active site: R59 (= R48), Y112 (= Y102), D184 (= D174), K209 (= K199)
binding S-ethyl-L-cysteine: Y112 (= Y102), K209 (= K199), V337 (≠ M324), S338 (= S325)

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
40% identity, 98% coverage: 2:381/386 of query aligns to 1:393/395 of 5m3zA

query
sites
5m3zA

N

D

T

C

K

R

N

T

I

Y

G

G

F

F

N

N

T

T

I

Q

C

I

T

V

H

H

F

A

G

G

E

Q

I

Q

K

P

D

D

Q

P

Q

S

F

T

K

G

G

A

A

L

I

S

S

T

P

P

I

I

Y

F

L

Q

S

T

T

S

S

T

Y

F

A

V

F

F

E

D

D

S

V

A

E

E

T

Q

K

G

R

A

-

A

-

R

-

F

-

A

-

L

-

E

-

E

-

S

-

G

-

Y

-

I

Y

Y

P

T

R
|
R

Y

L

F

G

N

N

T

-

P

P

N

T

Q

T

E

D

A

A

L

L

C

E

K

K

K

K

I

L

A

A

K

V

L

L

E

E

K

R

G

G

E

E

S

A

S

G

L

L

V

A

F

T

G

A

S

S

G
|
G

M
x
I

A

S

A

A

I

I

S

T

T

T

T

T

L

L

F

L

A

T

F

L

L

C

Q

Q

K

Q

G

G

D

D

H

H

I

I

I

V

L

S

Q

A

R

S

A

A

I

I

Y
|
Y

G

G

G
x
H

T

T

A

H

N

A

L

F

I

L

N

S

E

H

E

S

F

M

K

P

K

K

H

F

G

G

I

I

D

N

F

V

S

S

Y

F

V

V

D

D

S

A

L

A

S

K

I

P

E

E

D

E

F

I

E

R

N

A

K

A

I

M

Q

R

S

P

N

E

T

T

K

K

I

V

I

V

Y

Y

V

I

E
|
E

T

T

P

P

S

A

N

N

P

P

L

T

L

L

S

S

I

L

I

V

D

D

L

I

N

E

A

T

I

V

G

A

A

G

L

I

G

A

K

H

K

Q

N

Q

G

G

I

A

I

L

T

L

I

V

V

V

D
|
D

N

N

T
|
T

F

F

A

M

S

S

P

P

V

Y

N

C

Q

Q

N

Q

P

P

I

L

V

Q

L

L

G

G

I

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T
|
T

K
|
K

Y

Y

L

I

G

N

G

G

H

H

S

G

D

D

I

V

L

I

A

G

G

G

A

I

V

I

I

V

A

G

S

K

E

Q

K

E

N

F

V

I

N

D

Q

Q

I

A

-

R

F

F

E

V

V

G

A

L

K

K

N

D

F

I

-

T

G

G

G

G

N

C

L

M

S

S

D

P

F

F

T

N

V

A

W

W

M

L

L

T

E

L

R

R

S

G

I

V

K

K

T

T

L

L

G

G

I

I

R

R

V

M

E

E

R

R

Q

H

N

C

K

E

N

N

A

A

L

L

K

K

I

I

S

A

K

R

W

F

L

L

Q

E

K

G

N

H

E

P

D

S

I

I

S

T

T

R

V

V

Y

Y

Y

Y

P

P

G

G

L

L

K

S

S

S

H

H

P

P

N

Q

H

Y

E

E

L

L

A

G

K

Q

Q

R

Q

Q

M

M

K

S

G

L

Y

P

G

G

G

G

M

I

L

I

S

S

F

F

E

E

L

I

K

A

E

G

G

G

L

L

S

E

A

A

D

G

L

R

F

R

Q

M

K

I

N

N

-

S

L

V

K

E

L

L

I

C

K

L

S

L

S

A

M
x
V

S
|
S

L

L

A

G

G

D

V

T

E

E

S

T

T

L

I

I

L

Q

S

H

P

P

H

A

L

S

T

M

S

T

H

H

A

S

L

P

L

V

K

A

K

P

E

E

E

E

R

R

S

L

S

K

Q

A

G

G

I

I

K

T

D

D

E

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

E

D

K

P

E

E

D

D

I

I

I

I

H

N

D

D

I

L

L

E

Q

H

A

A

I

I

E

R

K

K

active site: R58 (= R48), Y111 (= Y102), D183 (= D174), K208 (= K199)
binding norleucine: Y111 (= Y102), H113 (≠ G104), K208 (= K199), V336 (≠ M324), S337 (= S325)
binding pyridoxal-5'-phosphate: G86 (= G77), I87 (≠ M78), Y111 (= Y102), E154 (= E145), D183 (= D174), T185 (= T176), S205 (= S196), T207 (= T198), K208 (= K199)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G77), I87 (≠ M78), Y111 (= Y102), D183 (= D174), S205 (= S196), T207 (= T198), K208 (= K199), V336 (≠ M324), S337 (= S325), R372 (= R360)

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
40% identity, 98% coverage: 2:381/386 of query aligns to 2:394/396 of 6egrA

query
sites
6egrA

N

D

T

C

K

R

N

T

I

Y

G

G

F

F

N

N

T

T

I

Q

C

I

T

V

H

H

F

A

G

G

E

Q

I

Q

K

P

D

D

Q

P

Q

S

F

T

K

G

G

A

A

L

I

S

S

T

P

P

I

I

Y

F

L

Q

S

T

T

S

S

T

Y

F

A

V

F

F

E

D

D

S

V

A

E

E

T

Q

K

G

R

A

-

A

-

R

-

F

-

A

-

L

-

E

-

E

-

S

-

G

-

Y

-

I

Y

Y

P

T

R

R

Y

L

F

G

N

N

T

-

P

P

N

T

Q

T

E

D

A

A

L

L

C

E

K

K

K

K

I

L

A

A

K

V

L

L

E

E

K

R

G

G

E

E

S

A

S

G

L

L

V

A

F

T

G

A

S

S

G
|
G

M
x
I

A

S

A

A

I

I

S

T

T

T

T

T

L

L

F

L

A

T

F

L

L

C

Q

Q

K

Q

G

G

D

D

H

H

I

I

I

V

L

S

Q

A

R

S

A

A

I

I

Y
|
Y

G

G

G

C

T

T

A

H

N

A

L

F

I

L

N

S

E

H

E

S

F

M

K

P

K

K

H

F

G

G

I

I

D

N

F

V

S

S

Y

F

V

V

D

D

S

A

L

A

S

K

I

P

E

E

D

E

F

I

E

R

N

A

K

A

I

M

Q

R

S

P

N

E

T

T

K

K

I

V

I

V

Y

Y

V

I

E
|
E

T

T

P

P

S

A

N

N

P

P

L

T

L

L

S

S

I

L

I

V

D

D

L

I

N

E

A

T

I

V

G

A

A

G

L

I

G

A

K

H

K

Q

N

Q

G

G

I

A

I

L

T

L

I

V

V

V

D
|
D

N

N

T
|
T

F
|
F

A

M

S

S

P

P

V

Y

N

C

Q

Q

N

Q

P

P

I

L

V

Q

L

L

G

G

I

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T
|
T

K
|
K

Y

Y

L

I

G

N

G

G

H

H

S

G

D

D

I

V

L

I

A

G

G

G

A

I

V

I

I

V

A

G

S

K

E

Q

K

E

N

F

V

I

N

D

Q

Q

I

A

-

R

F

F

E

V

V

G

A

L

K

K

N

D

F

I

-

T

G

G

G

G

N

C

L

M

S

S

D

P

F

F

T

N

V

A

W

W

M

L

L

T

E

L

R

R

S

G

I

V

K

K

T

T

L

L

G

G

I

I

R

R

V

M

E

E

R

R

Q

H

N

C

K

E

N

N

A

A

L

L

K

K

I

I

S

A

K

R

W

F

L

L

Q

E

K

G

N

H

E

P

D

S

I

I

S

T

T

R

V

V

Y

Y

Y

Y

P

P

G

G

L

L

K

S

S

S

H

H

P

P

N

Q

H

Y

E

E

L

L

A

G

K

Q

Q

R

Q

Q

M

M

K

S

G

L

Y

P

G

G

G

G

M

I

L

I

S

S

F

F

E

E

L

I

K

A

E

G

G

G

L

L

S

E

A

A

D

G

L

R

F

R

Q

M

K

I

N

N

-

S

L

V

K

E

L

L

I

C

K

L

S

L

S

A

M

V

S

S

L

L

A

G

G

D

V

T

E

E

S

T

T

L

I

I

L

Q

S

H

P

P

H

A

L

S

T

M

S

T

H

H

A

S

L

P

L

Y

K

A

K

P

E

E

E

E

R

R

S

L

S

K

Q

A

G

G

I

I

K

T

D

D

E

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

I

L

E

E

E

D

K

P

E

E

D

D

I

I

I

I

H

N

D

D

I

L

L

E

Q

H

A

A

I

I

E

R

K

K

binding pyridoxal-5'-phosphate: G87 (= G77), I88 (≠ M78), Y112 (= Y102), E155 (= E145), D184 (= D174), T186 (= T176), F187 (= F177), S206 (= S196), T208 (= T198), K209 (= K199)

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
40% identity, 98% coverage: 2:381/386 of query aligns to 2:394/396 of 4hf8A

query
sites
4hf8A

N

D

T

C

K

R

N

T

I

Y

G

G

F

F

N

N

T

T

I

Q

C

I

T

V

H

H

F

A

G

G

E

Q

I

Q

K

P

D

D

Q

P

Q

S

F

T

K

G

G

A

A

L

I

S

S

T

P

P

I

I

Y

F

L

Q

S

T

T

S

S

T

Y

F

A

V

F

F

E

D

D

S

V

A

E

E

T

Q

K

G

R

A

-

A

-

R

-

F

-

A

-

L

-

E

-

E

-

S

-

G

-

Y

-

I

Y

Y

P

T

R
|
R

Y

L

F

G

N

N

T

-

P

P

N

T

Q

T

E

D

A

A

L

L

C

E

K

K

K

K

I

L

A

A

K

V

L

L

E

E

K

R

G

G

E

E

S

A

S

G

L

L

V

A

F

T

G

A

S

S

G
|
G

M
x
I

A

S

A

A

I

I

S

T

T

T

T

T

L

L

F

L

A

T

F

L

L

C

Q

Q

K

Q

G

G

D

D

H

H

I

I

I

V

L

S

Q

A

R

S

A

A

I

I

Y
|
Y

G

G

G

C

T

T

A

H

N

A

L

F

I

L

N

S

E

H

E

S

F

M

K

P

K

K

H

F

G

G

I

I

D

N

F

V

S

R

Y

F

V

V

D

D

S

A

L

A

S

K

I

P

E

E

D

E

F

I

E

R

N

A

K

A

I

M

Q

R

S

P

N

E

T

T

K

K

I

V

I

V

Y

Y

V

I

E
|
E

T

T

P

P

S

A

N
|
N

P

P

L

T

L

L

S

S

I

L

I

V

D

D

L

I

N

E

A

T

I

V

G

A

A

G

L

I

G

A

K

H

K

Q

N

Q

G

G

I

A

I

L

T

L

I

V

V

V

D
|
D

N

N

T

T

F

F

A

M

S

S

P

P

V

Y

N

C

Q

Q

N

Q

P

P

I

L

V

Q

L

L

G

G

I

A

D

D

I

I

V

V

V

V

H

H

S
|
S

A

V

T

T

K
|
K

Y

Y

L

I

G

N

G

G

H

H

S

G

D

D

I

V

L

I

A

G

G

G

A

I

V

I

I

V

A

G

S

K

E

Q

K

E

N

F

V

I

N

D

Q

Q

I

A

-

R

F

F

E

V

V

G

A

L

K

K

N

D

F

I

-

T

G

G

G

G

N

C

L

M

S

S

D

P

F

F

T

N

V

A

W

W

M

L

L

T

E

L

R

R

S

G

I

V

K

K

T

T

L

L

G

G

I

I

R

R

V

M

E

E

R

R

Q

H

N

C

K

E

N

N

A

A

L

L

K

K

I

I

S

A

K

R

W

F

L

L

Q

E

K

G

N

H

E

P

D

S

I

I

S

T

T

R

V

V

Y

Y

Y

Y

P

P

G

G

L

L

K

S

S

S

H

H

P

P

N

Q

H

Y

E

E

L

L

A

G

K

Q

Q

R

Q

Q

M

M

K

S

G

L

Y

P

G

G

G

G

M

I

L

I

S

S

F

F

E

E

L

I

K

A

E

G

G

G

L

L

S

E

A

A

D

G

L

R

F

R

Q

M

K

I

N

N

-

S

L

V

K

E

L

L

I

C

K

L

S

L

S

A

M

V

S
|
S

L

L

A

G

G

D

V

T

E

E

S

T

T

L

I

I

L

Q

S

H

P

P

H

A

L

S

T

M

S

T

H

H

A

S

L

P

L

V

K

A

K

P

E

E

E

E

R

R

S

L

S

K

Q

A

G

G

I

I

K

T

D

D

E

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

E

D

K

P

E

E

D

D

I

I

I

I

H

N

D

D

I

L

L

E

Q

H

A

A

I

I

E

R

K

K

active site: R59 (= R48), Y112 (= Y102), D184 (= D174), K209 (= K199)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G77), I88 (≠ M78), Y112 (= Y102), E155 (= E145), N159 (= N149), D184 (= D174), S206 (= S196), K209 (= K199), S338 (= S325), R373 (= R360)

7ba4A Structure of cystathionine gamma-lyase from pseudomonas aeruginosa
41% identity, 97% coverage: 7:382/386 of query aligns to 6:374/377 of 7ba4A

query
sites
7ba4A

G

A

F

F

N

A

T

T

I

R

C

V

T

I

H

H

F

A

G

G

E

Q

I

A

K

P

D

D

Q

P

Q

S

F

T

K

G

G

A

A

I

I

M

S

P

P

P

I

I

Y

Y

L

A

S

N

T

S

S

T

Y

Y

A

I

F

-

E

-

D

-

V

-

E

-

T

-

K

-

R

-

Y

-

P

-

R

Q

Y

R

F

S

N

H

T

N

P

P

N

T

Q

R

E

W

A

A

L

L

C

E

K

R

K

C

I

V

A

A

K

D

L

L

E

E

K

G

G

G

E

T

S

Q

S

A

L

F

V

A

F

F

G

A

S

S

G
|
G

M
x
L

A

A

A

A

I

I

S

S

T

S

T

V

L

L

F

-

A

E

F

L

L

L

Q

D

K

A

G

G

D

S

H

H

I

I

I

V

L

S

Q

G

R

N

A

D

I

L

Y
|
Y

G

G

G

G

T

T

A

F

N

R

L

L

I

F

N

E

E

R

E

V

F

R

K

R

K

R

H

S

-

A

G

G

I

H

D

R

F

F

S

S

Y

F

V

V

D

D

S

P

L

T

S

D

I

L

E

Q

D

A

F

F

E

E

N

A

K

A

I

L

Q

T

S

P

N

E

T

T

K

R

I

M

I

V

Y

W

V

V

E

E

T

T

P

P

S

S

N

N

P

P

L

L

L

L

S

R

I

L

I

T

D

D

L

L

N

R

A

A

I

I

G

A

A

Q

L

L

G

C

K

R

K

A

N

R

G

G

I

I

I

I

T

S

I

V

V

A

D
|
D

N

N

T

T

F
|
F

A

A

S

S

P

P

V

Y

N

I

Q

Q

N

R

P

P

I

L

V

E

L

L

G

G

I

F

D

D

I

V

V

V

V

V

H

H

S
|
S

A

T

T
|
T

K
|
K

Y

Y

L

L

G

N

G

G

H

H

S

S

D

D

I

V

L

I

A

G

G

G

-

I

A

A

V

I

I

V

A

G

S

D

E

N

K

P

N

D

V

L

N

R

Q

E

I

R

F

L

E

G

V

F

A

L

K

Q

N

N

F

S

G

V

G

G

N

A

L

I

S

S

-

G

D

P

F

F

T

D

V

A

W

F

M

L

L

T

E

L

R

R

S

G

I

V

K

K

T

T

L

L

G

A

I

L

R

R

V

M

E

E

R

R

Q

H

N

C

K

S

N

N

A

A

L

L

K

A

I

L

S

A

K

Q

W

W

L

L

Q

E

K

R

N

Q

E

P

D

Q

I

V

S

A

T

R

V

V

Y

Y

Y

Y

P

P

G

G

L

L

K

A

S

S

H

H

P

P

N

Q

H

H

E

E

L

L

A

A

K

K

Q

R

Q

Q

M

M

K

R

G

G

Y

F

G

G

G

G

M

M

L

I

S

S

F

L

E

D

L

L

K

R

E

C

G

D

L

L

S

A

-

G

A

A

D

R

L

R

F

F

Q

L

K

E

N

N

L

V

K

R

L

I

I

F

K

S

S

L

S

A

M

E

S

S

L

L

A

G

G

G

V

V

E

E

S

S

T

L

I

I

L

E

S

H

P

P

H

A

L

I

T

M

S

T

H

H

A

A

L

S

L

I

K

P

K

A

E

E

E

T

R

R

S

A

S

D

Q

L

G

G

I

I

K

G

D

D

E

S

L

L

L

I

R

R

L

L

S

S

V

V

G

G

I

V

E

E

E

A

K

L

E

E

D

D

I

L

I

Q

H

A

D

D

I

L

L

A

Q

Q

A

A

I

L

E

A

K

K

V

I

binding pyridoxal-5'-phosphate: G66 (= G77), L67 (≠ M78), Y90 (= Y102), D163 (= D174), F166 (= F177), S185 (= S196), T187 (= T198), K188 (= K199)

7d7oB Crystal structure of cystathionine gamma-lyase from bacillus cereus atcc 14579 (see paper)
43% identity, 91% coverage: 29:379/386 of query aligns to 24:376/377 of 7d7oB

query
sites
7d7oB

P

P

I

I

Y

Y

L

Q

S

T

T

S

S

T

Y

Y

A

K

F

Q

E

E

D

A

V

V

E

G

T

K

K

H

R

Q

Y

G

P

Y

R

E

Y
|
Y

F

S

N
x
R

T

T

-

G

-

N

P

P

N

T

Q

R

E

A

A

A

L

L

C

E

K

E

K

M

I

I

A

A

K

V

L

L

E

E

K

N

G

G

E

H

S

A

S

G

L

F

V

A

F

F

G

G

S

S

G
|
G

M
|
M

A

A

A

A

I

I

S

T

T

A

T

T

L

I

F

M

A

L

F

F

L

-

Q

S

K

K

G

G

D

D

H

H

I

V

I

I

L

L

Q

T

R

D

A

D

I

V

Y
|
Y

G

G

G

G

T

T

A

Y

N

R

L

V

I

I

N

T

E

K

E

V

F

L

K

N

K

R

H

F

G

G

I

I

D

E

F

H

S

T

Y

F

V

V

D

D

S

T

L

T

S

N

I

L

E

E

D

E

F

V

E

E

N

E

K

A

I

I

Q

R

S

P

N

N

T

T

K

K

I

A

I

I

Y

Y

V

V

E

E

T

T

P

P

S

T

N

N

P

P

L

L

L

L

S

K

I

I

I

T

D

D

L

I

N

K

A

K

I

I

G

S

A

T

L

L

G

A

K

K

K

E

N

K

G

D

I

L

I

L

T

T

I

I

V

I

D
|
D

N

N

T
|
T

F

F

A

M

S

T

P

P

V

Y

N

W

Q

Q

N

S

P

P

I

I

V

S

L

L

G

G

I

A

D

D

I

I

V

V

V

L

H

H

S
|
S

A

A

T
|
T

K
|
K

Y

Y

L

L

G

G

G

G

H

H

S

S

D

D

I

V

L

V

A

A

G

G

A

L

V

V

I

V

A

V

S

N

E

S

K

P

N

Q

V

L

N

A

Q

E

I

D

F

L

E

H

V

F

A

V

K

Q

N

N

-

S

F

T

G

G

G

G

N

I

L

L

S

G

D

P

F

Q

T

D

V

S

W

F

M

L

L

L

E

L

R

R

S

G

I

L

K

K

T

T

L

L

G

G

I

I

R

R

V

M

E

E

R

E

Q

H

N

E

K

T

N

N

A

S

L

R

K

A

I

I

S

A

K

E

W

F

L

L

Q

N

K

N

N

H

E

P

D

K

I

V

S

N

T

K

V

V

Y

Y

Y

Y

P

P

G

G

L

L

K

E

S

S

H

H

P

Q

N

N

H

H

E

E

L

L

A

A

K

T

Q

E

Q

Q

M

A

K

N

G

G

Y

F

G

G

G

A

M

I

L

I

S

S

F

F

E

D

L

V

K

D

E

S

G

E

L

E

S

T

A

L

D

N

L

K

F

V

Q

L

K

E

N

K

L

L

K

Q

L

Y

I

F

K

T

S

L

S

A

M

E

S

S

L

L

A

G

G

A

V

V

E

E

S

S

T

L

I

I

L

S

S

I

P

P

H

S

L

Q

T

M

S

T

H

H

A

A

L

S

L

I

K

P

K

A

E

D

E

R

R

R

S

K

S

E

Q

L

G

G

I

I

K

T

D

D

E

T

L

L

L

I

R

R

L

I

S

S

V

V

G

G

I

I

E

E

E

D

K

G

E

E

D

D

I

L

I

I

H

E

D

D

I

L

L

A

Q

Q

A

A

I

L

binding pyridoxal-5'-phosphate: Y44 (= Y49), R46 (≠ N51), G74 (= G77), M75 (= M78), Y98 (= Y102), D170 (= D174), T172 (= T176), S192 (= S196), T194 (= T198), K195 (= K199)

5dx5A Crystal structure of methionine gamma-lyase from clostridium sporogenes (see paper)
38% identity, 99% coverage: 2:384/386 of query aligns to 2:399/399 of 5dx5A

query
sites
5dx5A

N

N

T

I

K

K

N

K

I

M

G

G

F

F

N

A

T

T

I

K

C

A

T

I

H

H

F

G

G

G

E

H

I

I

K

G

D

D

Q

K

Q

Q

F

F

K

G

G

S

A

L

I

A

S

T

P

P

I

I

Y

Y

L

Q

S

T

T

S

S

T

Y

F

A

I

F

F

E

D

D

S

V

A

E

E

T

Q

-

G

-

G

K

R

R

R

-

F

-

A

-

G

-

E

-

E

-

S

-

G

-

Y

-

I

Y
|
Y

P

S

R
|
R

Y

L

F

G

N

N

T

-

P

P

N

T

Q

S

E

T

A

E

L

V

C

E

K

N

K

K

I

L

A

A

K

L

L

L

E

E

K

G

G

G

E

E

S

A

S

A

L

V

V

V

F

A

G

A

S
|
S

G
|
G

M
|
M

A

G

A

A

I

I

S

A

T

A

T

S

L

L

F

W

A

S

F

A

L

L

Q

K

K

S

G

G

D

D

H

H

I

V

I

V

L

A

Q

S

R

D

A

T

I

L

Y
|
Y

G

G

G

C

T

T

A

F

N

A

L

L

I

L

N

N

E

H

E

G

F

L

K

T

K

R

H

Y

G

G

I

V

D

E

F

V

S

T

Y

F

V

V

D

D

S

V

L

S

S

N

I

L

E

D

D

E

F

V

E

K

N

N

K

A

I

L

Q

K

S

P

N

N

T

T

K

K

I

V

I

V

Y

Y

V

L

E

E

T

T

P

P

S

A

N

N

P

P

L

T

L

L

S

K

I

V

I

T

D

D

L

I

N

R

A

K

I

I

G

S

A

N

L

M

G

V

K

H

K

E

N

S

G

N

-

K

-

E

I

C

I

F

T

V

I

F

V

V

D
|
D

N

N

T

T

F
|
F

A

C

S

T

P

P

V

Y

N

I

Q

Q

N

R

P

P

I

L

V

E

L

L

G

G

I

A

D

D

I

V

V

V

V

V

H

H

S
|
S

A

A

T
|
T

K
|
K

Y

Y

L

L

G

N

G

G

H

H

S

G

D

D

I

V

L

I

A

A

G

G

A

F

V

A

I

V

A

G

S

K

E

E

K

E

N

F

V

I

N

N

Q

Q

I

V

-

K

-

L

F

F

E

G

V

I

A

K

K

D

N

M

F

T

G

G

G

S

N

V

L

T

S

G

D

P

F

F

T

E

V

S

W

F

M

L

L

I

E

I

R

R

S

G

I

M

K

K

T

T

L

L

G

Q

I

L

R

R

V

M

E

E

R

K

Q

H

N

C

K

K

N

N

A

A

L

M

K

E

I

V

S

A

K

K

W

F

L

L

Q

E

K

S

N

H

E

P

D

A

I

V

S

E

T

K

V

V

Y

Y

Y

Y

P

P

G

G

L