SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_062735310.1 NCBI__GCF_001580365.1:WP_062735310.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WQD1 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
50% identity, 96% coverage: 23:700/708 of query aligns to 36:647/651 of P9WQD1

query
sites
P9WQD1

R

R

V

L

A

A

F

F

W

W

E

A

G

K

Q

Q

A

A

S

N

R

R

L

L

D

S

W

W

A

T

G

T

P

P

W

F

T

T

T

E

A

V

H

L

R

D

F

W

E

S

G

G

P

-

R

-

R

-

I

-

G

-

Q

-

D

-

A

-

D

-

G

-

N

-

P

-

T

-

W

-

S

-

V

A

P

P

E

F

L

A

A

K

W

W

F

F

E

V

G

G

G

G

R

E

L

L

N

N

V

V

A

A

H

Y

N

N

C

C

V

V

D

D

R

R

H

H

V

V

E

E

A

A

G

G

R

H

G

G

A

D

H

R

V

V

A

A

L

I

Y

H

F

W

E

E

G

G

E

E

P

P

-

V

G

G

D

D

R

R

L

R

V

T

Y

L

T

T

Y

Y

A

S

D

D

L

L

Q

L

R

A

E

E

V

V

S

S

R

K

A

A

A

A

N

N

A

A

L

L

L

T

A

D

L

L

G

G

V

L

E

V

R

A

G

G

D

D

R

R

V

V

V

A

I

I

Y

Y

L

L

P

P

V

L

I

I

P

P

E

E

T

A

V

V

V

I

I

A

T

M

L

L

A

A

C

C

A

A

R

R

I

L

G

G

A

I

V

M

H

H

S

S

L

V

V

V

F

F

G

G

G

G

F

F

S

T

A

A

E

A

A

A

L

L

R

Q

F

A

R

R

V

I

E

V

D

D

T

A

G

Q

A

A

K

K

V

L

L

L

V

I

T

T

T

A

D

D

G

G

Q

Q

H

F

R

R

R

R

G

G

A

K

V

P

V

S

P

P

V

L

K

K

A

A

V

A

A

A

D

D

E

E

A

A

C

L

S

A

G

A

-

I

-

P

D

D

N

C

A

S

I

V

E

E

H

H

V

V

V

L

V

V

V

V

R

R

R

R

T

T

S

G

R

I

P

-

G

-

R

-

S

-

D

-

L

-

P

-

R

-

G

-

T

-

R

-

G

-

T

-

H

-

S

-

K

-

E

-

E

-

D

-

A

E

V

M

P

A

W

W

T

S

R

E

G

G

R

R

D

D

V

L

W

W

W

W

H

H

E

H

L

V

V

V

A

G

E

S

Q

A

P

S

D

P

V

A

H

H

E

T

A

P

E

E

A

P

F

F

D

D

A

S

E

E

S

H

P

P

L

L

F

F

I

L

I

L

Y

Y

T

T

S

S

G

G

T

T

T

T

G

G

R

K

P

P

K

K

G

G

L

I

V

M

H

H

T

T

M

S

G

G

G

G

Y

Y

L

L

V

T

Q

Q

A

C

A

C

Y

Y

T

T

H

M

A

R

L

T

L

I

F

F

D

D

L

V

L

K

P

P

D

D

V

-

R

-

D

-

D

-

E

-

G

-

R

-

L

-

R

-

P

-

D

-

E

-

L

-

S

-

T

-

V

-

N

-

D

-

P

-

A

-

K

-

V

-

D

-

S

S

T

D

V

V

H

F

W

W

C

C

T

T

A

A

D

D

L

I

A

G

W

W

V

V

T

T

A

G

H

H

T

T

Y

Y

E

G

I

V

Y

Y

G

G

P

P

L

L

V

C

N

N

G

G

V

V

S

T

E

E

V

V

I

L

Y

Y

E

E

G

G

T

T

P

P

N

D

T

T

P

P

H

D

W

R

G

H

R

R

H

H

F

F

E

Q

V

I

I

I

E

E

R

K

Y

Y

G

G

V

V

T

T

T

I

Y

Y

Y

Y

T

T

A

A

P

P

T

T

L

L

I

I

R

R

S

M

L

F

M

M

G

-

A

K

F

W

P

G

K

R

G

E

V

I

P

P

P

D

L

S

Y

H

N

D

L

L

N

S

S

S

V

L

R

R

L

L

L

L

G

G

S

S

V

V

G

G

E

E

S

P

V

I

N

N

P

P

E

E

A

A

W

W

R

R

W

W

L

Y

R

R

A

D

Q

V

L

I

G

G

R

G

D

G

E

R

V

T

P

P

F

L

V

V

D

D

T

T

W

W

W

W

Q

Q

S

T

E

E

T

T

G

G

A

S

T

A

V

M

M

I

S

S

P

P

R

L

P

P

H

G

D

I

P

-

Q

-

F

-

A

-

P

-

P

-

G

-

T

-

F

-

D

-

D

-

G

-

A

-

P

-

H

-

T

A

A

A

L

A

K

K

P

P

G

G

C

S

A

A

T

M

R

T

A

P

V

L

P

P

G

G

L

I

S

S

T

A

R

K

V

I

V

V

D

D

E

D

A

H

G

G

A

D

T

P

V

L

P

P

P

P

G

H

-

T

-

E

-

G

-

A

-

Q

-

H

V

V

Q

T

G

G

F

Y

V

L

V

V

V

L

D

D

R

Q

T

P

G

W

P

P

S

S

M

M

A

L

R

R

T

G

V

I

W

W

G

G

D

D

P

P

Q

A

R

R

Y

Y

L

W

S

H

S

S

Y

Y

W

W

Q

S

A

K

Y

F

A

S

E

D

Q

K

G

G

W

Y

F

Y

L

F

A

A

G

G

D

D

G

G

A

A

R

R

C

I

D

D

E

P

E

D

G

G

D

A

I

I

Y

W

I

V

L

L

G

G

R

R

V

I

D

D

D

D

V

V

I

M

N

N

V

V

S

S

G

G

H

H

R

R

L

I

S

S

T

T

I

A

E

E

I

V

E

E

S

S

A

A

L

L

V

V

T

A

H

H

P

S

W

G

V

V

V

A

E

E

A

A

G

A

A

V

A

V

P

G

V

V

R

T

D

D

E

E

L

T

T

T

G

T

H

Q

A

A

V

I

L

C

A

A

Y

F

V

V

V

V

L

L

T

-

P

-

E

R

A

A

E

N

R

Y

V

A

P

P

A

H

D

D

E

R

L

T

T

A

A

E

T

E

L

L

R

R

E

T

H

E

V

V

A

A

R

R

E

V

I

I

G

S

P

P

I

I

A

A

K

R

P

P

R

R

A

D

V

V

V

H

A

V

V

V

P

P

D

E

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

T

M

R

R

R

R

L

L

L

L

A

R

Q

D

L

V

W

A

E

E

G

N

E

R

P

E

L

L

G

G

D

D

R

T

S

S

S

T

L

L

Q

L

N

D

E

P

E

T

A

V

L

F

E

D

R

A

I

I

K617 (= K670) modified: N6-acetyllysine; mutation to R: Complete loss of acetyl-coenzyme A synthetase activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
40% identity, 98% coverage: 9:702/708 of query aligns to 19:643/652 of P27550

query
sites
P27550

L

I

S

N

P

P

T

Q

P

Q

N

Y

P

E

A

A

L

M

V

Y

S

Q

D

Q

A

S

A

I

R

N

V

V

-

P

-

D

A

T

F

F

W

W

E

G

G

E

Q

Q

A

G

S

K

R

I

L

L

D

D

W

W

A

I

G

K

P

P

W

Y

T

Q

T

K

A

V

H

-

R

-

F

-

E

-

G

-

P

-

R

-

R

K

I

N

G

T

Q

S

D

F

A

A

D

P

G

G

N

N

P

V

T

S

W

-

S

-

V

-

P

-

E

-

L

I

A

K

W

W

F

Y

E

E

G

D

G

G

R

T

L

L

N

N

V

L

A

A

H

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

R

N

G

G

A

D

H

R

V

T

A

A

L

I

Y

I

F

W

E

E

G

G

E

D

P

D

G

A

D

S

R

Q

L

S

V

K

Y

H

-

I

T

S

Y

Y

A

K

D

E

L

L

Q

H

R

R

E

D

V

V

S

C

R

R

A

F

A

A

N

N

A

T

L

L

L

L

A

E

L

L

G

G

V

I

E

K

R

K

G

G

D

D

R

V

V

V

V

A

I

I

Y

Y

L

M

P

P

V

M

I

V

P

P

E

E

T

A

V

A

V

V

I

A

T

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

L

V

V

I

F

F

G

G

G

G

F

F

S

S

A

P

E

E

A

A

L

V

R

A

F

G

R

R

V

I

E

I

D

D

T

S

G

N

A

S

K

R

V

L

L

V

V

I

T

T

T

S

D

D

G

E

Q

G

H

V

R

R

R

A

G

G

A

R

V

S

V

I

P

P

V

L

K

K

A

K

V

N

A

V

D

D

E

D

A

A

C

L

S

K

G

N

D

P

N

N

-

V

-

T

A

S

I

V

E

E

H

H

V

V

V

V

V

V

V

L

R

K

R

R

T

T

S

G

R

-

P

-

G

G

R

K

S

I

D

D

L

-

P

-

R

-

G

-

T

-

R

-

G

-

T

-

H

-

S

-

K

-

E

-

E

-

D

-

A

-

V

-

P

-

W

W

T

Q

R

E

G

G

R

R

D

D

V

L

W

W

W

W

H

H

E

D

L

L

V

V

A

E

E

Q

Q

A

P

S

D

D

V

Q

H

H

E

Q

A

A

E

E

A

E

F

M

D

N

A

A

E

E

S

D

P

P

L

L

F

F

I

I

I

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

R

K

P

P

K

K

G

G

L

V

V

L

H

H

T

T

M

T

G

G

G

G

Y

Y

L

L

V

V

Q

Y

A

A

A

A

Y

L

T

T

H

F

A

K

L

Y

L

V

F

F

D

D

L

Y

L

H

P

P

D

-

V

-

R

-

D

-

D

-

E

-

G

-

R

-

L

-

R

-

P

-

D

-

E

-

L

-

S

-

T

-

V

-

N

-

D

-

P

-

A

-

K

-

V

-

D

-

S

G

T

D

V

I

H

Y

W

W

C

C

T

T

A

A

D

D

L

V

A

G

W

W

V

V

T

T

A

G

H

H

T

S

Y

Y

E

L

I

L

Y

Y

G

G

P

P

L

L

V

A

N

C

G

G

V

A

S

T

E

T

V

L

I

M

Y

F

E

E

G

G

T

V

P

P

N

N

T

W

P

P

H

T

W

P

G

A

R

R

H

M

F

A

E

Q

V

V

I

V

E

D

R

K

Y

H

G

Q

V

V

T

N

T

I

Y

L

Y

Y

T

T

A

A

P

P

T

T

L

A

I

I

R

R

S

A

L

L

M

M

G

A

A

E

F

G

P

D

K

K

G

A

V

I

P

E

P

G

L

T

Y

-

N

D

L

R

N

S

S

S

V

L

R

R

L

I

L

L

G

G

S

S

V

V

G

G

E

E

S

P

V

I

N

N

P

P

E

E

A

A

W

W

R

E

W

W

L

Y

R

W

A

K

Q

K

L

I

G

G

R

N

D

E

E

K

V

C

P

P

F

V

V

V

D

D

T

T

W

W

W

W

Q

Q

S

T

E

E

T

T

G

G

A

G

T

F

V

M

M

I

S

T

P

P

R

L

P

P

H

-

D

-

P

-

Q

-

F

-

A

-

P

-

P

-

G

-

T

-

F

-

D

-

D

-

G

G

A

A

P

-

H

-

T

T

A

E

L

L

K

K

P

A

G

G

C

S

A

A

T

T

R

R

A

P

V

F

P

F

G

G

L

V

S

Q

T

P

R

A

V

L

V

V

D

D

E

N

A

E

G

G

A

N

T

P

V

L

P

E

P

G

G

A

V

T

Q

E

G

G

F

S

V

L

V

V

V

I

D

T

R

D

T

S

G

W

P

P

S

G

M

Q

A

A

R

R

T

T

V

L

W

F

G

G

D

D

P

H

Q

E

R

R

Y

F

L

E

S

Q

S

T

Y

Y

W

F

Q

S

A

T

Y

F

A

-

E

-

Q

K

G

N

W

M

F

Y

L

F

A

S

G

G

D

D

G

G

A

A

R

R

C

R

D

D

E

E

E

D

G

G

D

Y

I

Y

Y

W

I

I

L

T

G

G

R

R

V

V

D

D

D

D

V

V

I

L

N

N

V

V

S

S

G

G

H

H

R

R

L

L

S

G

T

T

I

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

T

A

H

H

P

P

W

K

V

I

V

A

E

E

A

A

G

A

A

V

A

V

P

G

V

I

R

P

D

H

E

N

L

I

T

K

G

G

H

Q

A

A

V

I

L

Y

A

A

Y

Y

V

V

V

T

L

L

T

N

P

H

E

G

A

E

E

E

R

-

V

-

P

P

A

S

D

P

E

E

L

L

T

Y

A

A

T

E

L

V

R

R

E

N

H

W

V

V

A

R

R

K

E

E

I

I

G

G

P

P

I

L

A

A

K

T

P

P

R

D

A

V

V

L

V

H

A

W

V

T

P

D

D

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

T

M

R

R

R

R

L

I

L

L

A

R

Q

K

L

I

W

A

E

A

G

G

E

D

P

T

-

S

-

N

L

L

G

G

D

D

R

T

S

S

S

T

L

L

Q

A

N

D

E

P

E

G

A

V

L

V

E

E

R

K

I

L

A

L

E

E

K609 (= K670) modified: N6-acetyllysine; by autocatalysis

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
40% identity, 96% coverage: 26:702/708 of query aligns to 34:637/641 of 2p20A

query
sites
2p20A

F

F

W

W

E

G

G

E

Q

Q

A

G

S

K

R

I

L

L

D

D

W

W

A

I

G

T

P

P

W

Y

T

Q

T

K

A

V

H

-

R

-

F

-

E

-

G

-

P

-

R

-

R

K

I

N

G

T

Q

S

D

F

A

A

D

P

G

G

N

N

P

V

T

S

W

-

S

-

V

-

P

-

E

-

L

I

A

K

W

W

F

Y

E

E

G

D

G

G

R

T

L

L

N

N

V

L

A

A

H

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

R

N

G

G

A

D

H

R

V

T

A

A

L

I

Y

I

F

W

E

E

G

G

E

D

P

D

G

T

D

S

R

Q

L

S

V

K

Y

H

-

I

T

S

Y

Y

A

R

D

E

L

L

Q

H

R

R

E

D

V

V

S

C

R

R

A

F

A

A

N

N

A

T

L

L

L

L

A

D

L

L

G

G

V

I

E

K

R

K

G

G

D

D

R

V

V

V

V

A

I

I

Y

Y

L

M

P

P

V

M

I

V

P

P

E

E

T

A

V

A

V

V

I

A

T

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

L

V

V

I

F

F

G

G

G

G

F

F

S

S

A

P

E

E

A

A

L

V

R

A

F

G

R

R

V

I

E

I

D

D

T

S

G

S

A

S

K

R

V

L

L

V

V

I

T

T

T

A

D

D

G

E

Q

G

H

V

R

R

R

A

G

G

A

R

V

S

V

I

P

P

V

L

K

K

A

K

V

N

A

V

D

D

E

D

A

A

C

L

S

K

G

N

D

P

N

N

-

V

-

T

A

S

I

V

E

E

H

H

V

V

V

I

V

V

V

L

R

K

R

R

T

T

S

G

R

-

P

-

G

-

R

-

S

S

D

D

L

-

P

-

R

-

G

-

T

-

R

-

G

-

T

-

H

-

S

-

K

-

E

-

E

-

D

-

A

-

V

I

P

D

W

W

T

Q

R

E

G

G

R

R

D

D

V

L

W

W

W

W

H

R

E

D

L

L

V

I

A

E

E

K

Q

A

P

S

D

P

V

E

H

H

E

Q

A

P

E

E

A

A

F

M

D

N

A

A

E

E

S

D

P

P

L

L

F

F

I

I

I

L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

R

K

P

P

K

K

G

G

L

V

V

L

H

H

T

T

M

T

G

G

G

G

Y

Y

L

L

V

V

Q

Y

A

A

A

A

Y

T

T

T

H

F

A

K

L

Y

L

V

F

F

D

D

L

Y

L

H

P

P

D

-

V

-

R

-

D

-

D

-

E

-

G

-

R

-

L

-

R

-

P

-

D

-

E

-

L

-

S

-

T

-

V

-

N

-

D

-

P

-

A

-

K

-

V

-

D

-

S

G

T

D

V

I

H

Y

W

W

C

C

T

T

A

A

D

D

L

V

A

G

W

W

V

V

T

T

A

G

H

H

T

S

Y

Y

E

L

I

L

Y

Y

G

G

P

P

L

L

V

A

N

C

G

G

V

A

S

T

E

T

V

L

I

M

Y

F

E

E

G

G

T

V

P

P

N

N

T

W

P

P

H

T

W

P

G

A

R

R

H

M

F

C

E

Q

V

V

I

V

E

D

R

K

Y

H

G

Q

V

V

T

N

T

I

Y

L

Y

Y

T

T

A

A

P

P

T

T

L

A

I

I

R

R

S

A

L

L

M

M

G

A

A

E

F

G

P

D

K

K

G

A

V

I

P

E

P

G

L

T

Y

-

N

D

L

R

N

S

S

S

V

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

S
x
P

V

I

N

N

P

P

E

E

A

A

W

W

R

E

W

W

L

Y

R

W

A

K

Q

K

L

I

G

G

R

K

D

E

E

K

V

C

P

P

F

V

V

V

D

D

T
|
T

W
|
W

W
|
W

Q
|
Q

S
x
T

E
|
E

T

T

G

G

A

G

T

F

V

M

M

I

S

T

P

P

R

L

P

P

H

-

D

-

P

-

Q

-

F

-

A

-

P

-

P

-

G

-

T

-

F

-

D

-

D

-

G

G

A

A

P

-

H

-

T

I

A

E

L

L

K

K

P

A

G

G

C

S

A

A

T

T

R

R

A

P

V

F

P

F

G

G

L

V

S

Q

T

P

R

A

V

L

V

V

D

D

E

N

A

E

G

G

A

H

T

P

V

Q

P

E

P

G

G

A

V

T

Q

E

G

G

F

N

V

L

V

V

V

I

D

T

R

D

T

S

G

W

P

P

S

G

M

Q

A

A

R

R

T

T

V

L

W

F

G

G

D

D

P

H

Q

E

R

R

Y

F

L

E

S

Q

S

T

Y

Y

W

F

Q

S

A

T

Y

F

A

-

E

-

Q

K

G

N

W

M

F

Y

L

F

A

S

G

G

D
|
D

G

G

A

A

R

R

C

R

D

D

E

E

E

D

G

G

D

Y

I

Y

Y

W

I
|
I

L

T

G

G

R
|
R

V

V

D

D

D

D

V

V

I

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

L

L

S

G

T

T

I

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

T

A

H

H

P

P

W

K

V

I

V

A

E

E

A

A

G

A

A

V

A

V

P

G

V

I

R

P

D

H

E

A

L

I

T

K

G

G

H

Q

A

A

V

I

L

Y

A

A

Y

Y

V

V

V

T

L

L

T

N

P

H

E

G

A

E

E

E

R

-

V

-

P

P

A

S

D

P

E

E

L

L

T

Y

A

A

T

E

L

V

R

R

E

N

H

W

V

V

A

A

R

K

E

E

I

I

G

G

P

P

I

L

A

A

K

T

P

P

R

D

A

V

V

L

V

H

A

W

V

T

P

D

D

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

T

M

R

R

R

R

L

I

L

L

A

R

Q

K

L

I

W

A

E

A

G

G

E

D

P

T

L

S

G

N

D

D

R

T

S

S

S

T

L

L

Q

A

N

D

E

P

E

G

A

V

L

V

E

E

R

K

I

L

A

L

E

E

active site: T260 (= T281), T412 (≠ S458), E413 (= E459), N517 (= N580), R522 (= R585), K605 (= K670)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G429), E384 (= E430), P385 (≠ S431), T408 (= T454), W409 (= W455), W410 (= W456), Q411 (= Q457), T412 (≠ S458), D496 (= D559), I508 (= I571), R511 (= R574), R522 (= R585)

8rplB Amp-forming acetyl-coa synthetase from chloroflexota bacterium with bound acetyl amp (see paper)
41% identity, 94% coverage: 19:687/708 of query aligns to 38:628/630 of 8rplB

query
sites
8rplB

S

S

D

L

A

A

A

N

R

R

V

F

A

E

F

F

W

W

E

N

G

D

Q

M

A

A

S

K

R

E

L

L

D

H

W

W

A

F

G

E

P

P

W

W

T

K

T

S

A

T

H

-

R

-

F

F

E

E

G

W

P

T

R

D

R

K

I

-

G

-

Q

-

D

-

A

-

D

-

G

-

N

-

P

-

T

-

W

-

S

-

V

-

P

P

E

F

L

F

A

K

W

W

F

F

E

T

G

D

G

G

R

K

L

F

N

N

V

I

A

A

H

Y

N

N

C

C

V

L

D

D

R

R

H

Y

V

M

E

G

A

T

G

P

R

I

G

E

A

D

H

K

V

V

A

A

L

F

Y

Y

F

W

E

E

G

G

E

D

P

D

G

G

D

S

R

S

L

R

V

A

Y

Y

T

T

Y

Y

A

K

D

E

L

M

Q

Y

R

V

E

L

V

T

S

N

R

R

A

V

A

A

N

K

A

V

L

L

L

Q

A

N

L

Q

G

G

V

V

E

K

R

K

G

G

D

D

R

R

V

V

V

A

I

I

Y

Y

L

M

P

P

V

M

I

I

P

P

E

E

T

M

V

A

V

A

I

S

T

V

L

L

A

A

C

C

A

A

R

R

I

L

G

G

A

A

V

P

H

H

S

M

L

V

V

V

F

F

G

G

G

G

F

F

S

A

A

A

E

S

A

S

L

L

R

R

F

D

R

R

V

M

E

N

D

D

T

C

G

D

A

A

K

K

V

V

L

L

V

I

T

T

T

A

D

D

G

G

Q

G

H

Y

R

R

R

G

G

G

A

K

V

V

V

I

P

E

V

L

K

K

A

K

V

I

A

A

D

D

E

E

A

A

C

V

S

A

G

E

D

T

N

P

A

T

I

I

E

E

H

K

V

V

V

F

V

V

V

Q

R

R

R

H

T

T

S

G

R

F

P

-

G

-

R

-

S

-

D

-

L

-

P

-

R

-

G

-

T

-

R

-

G

-

T

-

H

-

S

-

K

-

E

-

E

-

D

-

A

E

V

V

P

P

W

M

T

A

R

E

G

G

R

R

D

D

V

V

W

Y

W

L

H

D

E

V

L

L

V

L

A

N

E

D

Q

I

P

P

D

E

-

D

-

T

V

V

H

V

E

P

A

C

E

E

A

P

F

V

D

D

A

S

E

E

S

D

P

M

L

L

F

Y

I

I

I

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

R

K

P

P

K

K

G

G

L

V

V

V

H

H

T

V

M

H

G

G

G

G

Y

Y

L

A

V

V

Q

G

A

C

A

Y

Y

A

T

T

H

T

A

K

L

F

L

V

F

F

D

D

L

I

L

K

P

P

D

-

V

-

R

-

D

-

D

-

E

-

G

-

R

-

L

-

R

-

P

-

D

-

E

-

L

-

S

-

T

-

V

-

N

-

D

-

P

-

A

-

K

-

V

-

D

-

S

S

T

D

V

V

H

F

W

W

C

C

T

T

A

A

D

D

L

I

A

G

W

W

V

V

T

T

A

G

H

H

T

S

Y

Y

E

T

I

I

Y

Y

G

G

P

P

L

M

V

M

N

N

G

A

V

A

S

S

E

I

V

V

I

L

Y

F

E

E

G

G

T

I

P

P

N

T

T

Y

P

P

H

A

W

A

G

D

R

R

H

F

F

W

E

S

V

I

I

V

E

E

R

K

Y

Y

G

K

V

V

T

N

T

I

Y

I

Y

Y

T

T

A

A

P

P

T

T

L

A

I

I

R

R

S

S

L

L

M

M

G

-

A

R

F

F

P

G

K

E

G

E

V

L

P

P

P

A

L

R

Y

H

N

D

L

L

N

S

S

S

V

L

R

R

L

I

L

L

G

G

S

T

V

V

G
|
G

E
|
E

S
x
P

V

I

N

N

P

P

E

E

A

A

W

W

R

M

W

W

L

Y

R

R

A

K

Q

N

L

I

G

G

R

H

D

N

E

E

V

L

P

P

F

I

V

M

D

D

T
|
T

W
|
W

W
|
W

Q
|
Q

S
x
T

E

E

T

T

G

G

A

M

T

I

V

L

M

I

S

S

P

P

R

T

P

P

H

I

D

L

P

P

Q

-

F

-

A

-

P

-

P

-

G

-

T

-

F

-

D

-

D

-

G

-

A

-

P

-

H

-

T

-

A

-

L

L

K

K

P

P

G

G

C

S

A

A

T

S

R

R

A

P

V

L

P

P

G

T

L

I

S

E

T

A

R

D

V

V

V

V

D

N

E

K

A

D

G

G

A

K

T

P

V

V

P

G

P

P

G

E

V

H

Q

G

G

G

F

F

V

L

V

I

V

I

D

R

R

H

T

P

G

W

P

P

S

A

M

Q

A

M

R

R

T

T

V

I

W

F

G

G

D

D

P

P

Q

D

R

R

Y

Y

L

-

S

K

S

T

Y

Y

W

W

Q

E

A

T

Y

I

A

P

E

D

Q

V

G

-

W

-

F

Y

L

F

A

A

G

G

D
|
D

G

A

A

A

R

T

C

M

D

D

E

K

E

M

G

G

D

Y

I

F

Y

R

I

I

L

Q

G

G

R
|
R

V

V

D

D

D

D

V

V

I

I

N
x
K

V

V

S

S

G

G

H

H

R
|
R

L

L

S

G

T

S

I

M

E

E

I

I

E

E

S

S

A

S

L

L

V
|
V

T

S

H
|
H

P

P

W

A

V

V

V

A

E

E

A

A

G

A

A

A

A

I

P

G

V

K

R

P

D

D

E

E

L

V

T

K

G

G

H

E

A

H

V

V

L

K

A

V

Y

F

V

V

V

I

L

L

T

R

P

N

E

G

A

V

E

E

R

-

V

-

P

P

A

T

D

E

E

S

L

L

T

A

A

V

T

E

L

L

R

K

E

R

H

H

V

V

A

R

R

T

E

L

I

V

G

G

P

P

I

L

A

A

K

T

P

P

R

D

A

E

V

L

V

E

A

F

V

V

P

T

D

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K

K

I

I

T

M

R

R

R

R

L

V

L

V

A

R

Q

A

L

R

W

E

E

L

G

G

E

E

P

P

L

V

G

G

D

D

binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] ethanoate: G391 (= G429), E392 (= E430), P393 (≠ S431), T416 (= T454), W417 (= W455), W418 (= W456), Q419 (= Q457), T420 (≠ S458), D502 (= D559), R517 (= R574), K523 (≠ N580), R528 (= R585)
binding magnesium ion: V539 (= V596), H541 (= H598)

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
40% identity, 96% coverage: 26:702/708 of query aligns to 34:636/640 of 5jrhA

query
sites
5jrhA

F

F

W

W

E

G

G

E

Q

Q

A

G

S

K

R

I

L

L

D

D

W

W

A

I

G

T

P

P

W

Y

T

Q

T

K

A

V

H

-

R

-

F

-

E

-

G

-

P

-

R

-

R

K

I

N

G

T

Q

S

D

F

A

A

D

P

G

G

N

N

P

V

T

S

W

-

S

-

V

-

P

-

E

-

L

I

A

K

W

W

F

Y

E

E

G

D

G

G

R

T

L

L

N

N

V

L

A

A

H

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

R

N

G

G

A

D

H

R

V

T

A

A

L

I

Y

I

F
x
W

E

E

G

G

E

D

P

D

G

T

D

S

R

Q

L

S

V

K

Y

H

-

I

T

S

Y

Y

A

R

D

E

L

L

Q

H

R

R

E

D

V

V

S

C

R

R

A

F

A

A

N

N

A

T

L

L

L

L

A

D

L

L

G

G

V

I

E

K

R

K

G

G

D

D

R

V

V

V

V

A

I

I

Y

Y

L

M

P

P

V

M

I

V

P

P

E
|
E

T

A

V

A

V

V

I

A

T

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

L

V

V

I

F
|
F

G
|
G

G
|
G

F

F

S

S

A

P

E

E

A

A

L

V

R

A

F
x
G

R

R

V

I

E

I

D

D

T

S

G

S

A

S

K

R

V

L

L

V

V

I

T

T

T

A

D

D

G

E

Q

G

H

V

R
|
R

R

A

G

G

A

R

V

S

V

I

P

P

V

L

K

K

A

K

V

N

A

V

D

D

E

D

A

A

C

L

S

K

G

N

D

P

N

N

-

V

-

T

A

S

I

V

E

E

H

H

V

V

V

I

V

V

V

L

R

K

R

R

T

T

S

G

R

-

P

-

G

-

R

-

S

S

D

D

L

-

P

-

R

-

G

-

T

-

R

-

G

-

T

-

H

-

S

-

K

-

E

-

E

-

D

-

A

-

V

I

P

D

W

W

T

Q

R

E

G

G

R

R

D

D

V

L

W

W

W

W

H

R

E

D

L

L

V

I

A

E

E

K

Q

A

P

S

D

P

V

E

H

H

E

Q

A

P

E

E

A

A

F

M

D

N

A

A

E

E

S

D

P

P

L

L

F

F

I

I

I

L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

R
x
K

P
|
P

K

K

G

G

L

V

V

L

H

H

T

T

M

T

G

G

G

G

Y

Y

L

L

V

V

Q

Y

A

A

A

A

Y

T

T

T

H

F

A

K

L

Y

L

V

F

F

D

D

L

Y

L

H

P

P

D

-

V

-

R

-

D

-

D

-

E

-

G

-

R

-

L

-

R

-

P

-

D

-

E

-

L

-

S

-

T

-

V

-

N

-

D

-

P

-

A

-

K

-

V

-

D

-

S

G

T

D

V

I

H

Y

W

W

C

C

T

T

A

A

D

D

L

V

A

G

W

W

V

V

T

T

A

G

H

H

T

S

Y

Y

E

L

I

L

Y

Y

G

G

P

P

L

L

V

A

N

C

G

G

V

A

S

T

E

T

V

L

I

M

Y

F

E

E

G

G

T

V

P

P

N

N

T

W

P

P

H

T

W

P

G

A

R

R

H

M

F

C

E

Q

V

V

I

V

E

D

R

K

Y

H

G

Q

V

V

T

N

T

I

Y

L

Y

Y

T

T

A

A

P

P

T

T

L

A

I

I

R

R

S

A

L

L

M

M

G

A

A

E

F

G

P

D

K

K

G

A

V

I

P

E

P

G

L

T

Y

-

N

D

L

R

N

S

S

S

V

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

S
x
P

V

I

N

N

P

P

E

E

A

A

W

W

R

E

W

W

L

Y

R

W

A

K

Q

K

L

I

G

G

R

K

D

E

E

K

V

C

P

P

F

V

V

V

D

D

T
|
T

W
|
W

W
|
W

Q
|
Q

S
x
T

E
|
E

T

T

G

G

A

G

T

F

V

M

M

I

S

T

P

P

R

L

P

P

H

-

D

-

P

-

Q

-

F

-

A

-

P

-

P

-

G

-

T

-

F

-

D

-

D

-

G

G

A

A

P

-

H

-

T

I

A

E

L

L

K

K

P

A

G

G

C

S

A

A

T

T

R

R

A

P

V

F

P

F

G

G

L

V

S

Q

T

P

R

A

V

L

V

V

D

D

E

N

A

E

G

G

A

H

T

P

V

Q

P

E

P

G

G

A

V

T

Q

E

G

G

F

N

V

L

V

V

V

I

D

T

R

D

T

S

G

W

P

P

S

G

M

Q

A

A

R

R

T

T

V

L

W

F

G

G

D

D

P

H

Q

E

R

R

Y

F

L

E

S

Q

S

T

Y

Y

W

F

Q

S

A

T

Y

F

A

-

E

-

Q

K

G

N

W

M

F

Y

L

F

A

S

G

G

D
|
D

G

G

A

A

R

R

C

R

D

D

E

E

E

D

G

G

D

Y

I

Y

Y

W

I
|
I

L

T

G

G

R

R

V

V

D

D

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

L

L

S

G

T

T

I

A

E

E

I

I

E

E

S

S

A

A

L

L

V
|
V

T

A

H
|
H

P

P

W

K

V
x
I

V

A

E

E

A

A

G

A

A

V

A

V

P

G

V

I

R

P

D

H

E

A

L

I

T

K

G

G

H

Q

A

A

V

I

L

Y

A

A

Y

Y

V

V

V

T

L

L

T

N

P

H

E

G

A

E

E

E

R

-

V

-

P

P

A

S

D

P

E

E

L

L

T

Y

A

A

T

E

L

V

R

R

E

N

H

W

V

V

A
x
R

R

K

E

E

I

I

G

G

P
|
P

I

L

A

A

K

T

P

P

R

D

A

V

V

L

V

H

A

W

V

T

P

D

D

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

T

M

R

R

R

R

L

I

L

L

A

R

Q

K

L

I

W

A

E

A

G

G

E

D

P

-

L

L

G

G

D

D

R

T

S

S

S

T

L

L

Q

A

N

D

E

P

E

G

A

V

L

V

E

E

R

K

I

L

A

L

E

E

active site: T260 (= T281), T412 (≠ S458), E413 (= E459), N517 (= N580), R522 (= R585), K605 (= K670)
binding (r,r)-2,3-butanediol: W93 (≠ F98), E140 (= E144), G169 (≠ F173), K266 (≠ R287), P267 (= P288)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G429), E384 (= E430), P385 (≠ S431), T408 (= T454), W409 (= W455), W410 (= W456), Q411 (= Q457), T412 (≠ S458), D496 (= D559), I508 (= I571), N517 (= N580), R522 (= R585)
binding coenzyme a: F159 (= F163), G160 (= G164), G161 (= G165), R187 (= R191), S519 (= S582), R580 (≠ A645), P585 (= P650)
binding magnesium ion: V533 (= V596), H535 (= H598), I538 (≠ V601)

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
40% identity, 96% coverage: 26:702/708 of query aligns to 38:643/652 of Q8ZKF6

query
sites
Q8ZKF6

F

F

W

W

E

G

G

E

Q

Q

A

G

S

K

R

I

L

L

D

D

W

W

A

I

G

T

P

P

W

Y

T

Q

T

K

A

V

H

-

R

-

F

-

E

-

G

-

P

-

R

-

R

K

I

N

G

T

Q

S

D

F

A

A

D

P

G

G

N

N

P

V

T

S

W

-

S

-

V

-

P

-

E

-

L

I

A

K

W

W

F

Y

E

E

G

D

G

G

R

T

L

L

N

N

V

L

A

A

H

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

R

N

G

G

A

D

H

R

V

T

A

A

L

I

Y

I

F

W

E

E

G

G

E

D

P

D

G

T

D

S

R

Q

L

S

V

K

Y

H

-

I

T

S

Y

Y

A

R

D

E

L

L

Q

H

R

R

E

D

V

V

S

C

R

R

A

F

A

A

N

N

A

T

L

L

L

L

A

D

L

L

G

G

V

I

E

K

R

K

G

G

D

D

R

V

V

V

V

A

I

I

Y

Y

L

M

P

P

V

M

I

V

P

P

E

E

T

A

V

A

V

V

I

A

T

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

L

V

V

I

F

F

G

G

G

G

F

F

S

S

A

P

E

E

A

A

L

V

R

A

F

G

R

R

V

I

E

I

D

D

T

S

G

S

A

S

K

R

V

L

L

V

V

I

T

T

T

A

D

D

G

E

Q

G

H

V

R

R

R

A

G

G

A
x
R

V

S

V

I

P

P

V

L

K

K

A

K

V

N

A

V

D

D

E

D

A

A

C

L

S

K

G

N

D

P

N

N

-

V

-

T

A

S

I

V

E

E

H

H

V

V

V

I

V

V

V

L

R

K

R

R

T

T

S

G

R

-

P

-

G

-

R

-

S

S

D

D

L

-

P

-

R

-

G

-

T

-

R

-

G

-

T

-

H

-

S

-

K

-

E

-

E

-

D

-

A

-

V

I

P

D

W

W

T

Q

R

E

G

G

R

R

D

D

V

L

W

W

W

W

H

R

E

D

L

L

V

I

A

E

E

K

Q

A

P

S

D

P

V

E

H

H

E

Q

A

P

E

E

A

A

F

M

D

N

A

A

E

E

S

D

P

P

L

L

F

F

I

I

I

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

R

K

P

P

K

K

G

G

L

V

V

L

H

H

T

T

M

T

G

G

G

G

Y

Y

L

L

V

V

Q

Y

A

A

A

A

Y

T

T

T

H

F

A

K

L

Y

L

V

F

F

D

D

L

Y

L

H

P

P

D

-

V

-

R

-

D

-

D

-

E

-

G

-

R

-

L

-

R

-

P

-

D

-

E

-

L

-

S

-

T

-

V

-

N

-

D

-

P

-

A

-

K

-

V

-

D

-

S

G

T

D

V

I

H

Y

W

W

C

C

T

T

A

A

D

D

L

V

A

G

W

W

V

V

T
|
T

A

G

H

H

T

S

Y

Y

E

L

I

L

Y

Y

G

G

P

P

L

L

V

A

N

C

G

G

V

A

S

T

E

T

V

L

I

M

Y

F

E

E

G

G

T

V

P

P

N
|
N

T

W

P

P

H

T

W

P

G

A

R

R

H

M

F

C

E

Q

V

V

I

V

E

D

R

K

Y

H

G

Q

V

V

T

N

T

I

Y

L

Y

Y

T

T

A
|
A

P

P

T

T

L

A

I

I

R

R

S

A

L

L

M

M

G

A

A

E

F

G

P

D

K

K

G

A

V

I

P

E

P

G

L

T

Y

-

N

D

L

R

N

S

S

S

V

L

R

R

L

I

L

L

G

G

S

S

V

V

G

G

E

E

S

P

V

I

N

N

P

P

E

E

A

A

W

W

R

E

W

W

L

Y

R

W

A

K

Q

K

L

I

G

G

R

K

D

E

E

K

V

C

P

P

F

V

V

V

D

D

T

T

W

W

W

W

Q

Q

S

T

E

E

T

T

G

G

A

G

T

F

V

M

M

I

S

T

P

P

R

L

P

P

H

-

D

-

P

-

Q

-

F

-

A

-

P

-

P

-

G

-

T

-

F

-

D

-

D

-

G

G

A

A

P

-

H

-

T

I

A

E

L

L

K

K

P

A

G

G

C

S

A

A

T

T

R

R

A

P

V

F

P

F

G

G

L

V

S

Q

T

P

R

A

V

L

V

V

D

D

E

N

A

E

G

G

A

H

T

P

V

Q

P

E

P

G

G

A

V

T

Q

E

G

G

F

N

V

L

V

V

V

I

D

T

R

D

T

S

G

W

P

P

S

G

M

Q

A

A

R

R

T

T

V

L

W

F

G

G

D

D

P

H

Q

E

R

R

Y

F

L

E

S

Q

S

T

Y

Y

W

F

Q

S

A

T

Y

F

A

-

E

-

Q

K

G

N

W

M

F

Y

L

F

A

S

G

G

D

D

G

G

A

A

R

R

C

R

D

D

E

E

E

D

G

G

D

Y

I

Y

Y

W

I

I

L

T

G

G

R

R

V

V

D
|
D

D

D

V

V

I

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

L

L

S

G

T

T

I

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

T

A

H

H

P

P

W

K

V

I

V

A

E

E

A

A

G

A

A

V

A

V

P

G

V

I

R

P

D

H

E

A

L

I

T

K

G

G

H

Q

A

A

V

I

L

Y

A

A

Y

Y

V

V

V

T

L

L

T

N

P

H

E

G

A

E

E

E

R

-

V

-

P

P

A

S

D

P

E

E

L

L

T

Y

A

A

T

E

L

V

R

R

E

N

H

W

V

V

A
x
R

R

K

E

E

I

I

G

G

P

P

I

L

A

A

K

T

P

P

R

D

A

V

V

L

V

H

A

W

V

T

P

D

D

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

T

M

R

R

R

R

L

I

L

L

A

R

Q

K

L

I

W

A

E

A

G

G

E

D

P

T

-

S

-

N

L

L

G

G

D

D

R

T

S

S

S

T

L

L

Q

A

N

D

E

P

E

G

A

V

L

V

E

E

R

K

I

L

A

L

E

E

R194 (≠ A194) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T352) binding CoA
N335 (= N376) binding CoA
A357 (= A398) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D576) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S582) binding CoA
G524 (= G583) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R585) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ A645) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K670) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
42% identity, 96% coverage: 26:702/708 of query aligns to 37:640/648 of Q89WV5

query
sites
Q89WV5

F

F

W

W

E

A

G

E

Q

Q

A

A

S

K

R

R

L

I

D

D

W

W

A

-

G

-

P

-

W

-

T

-

T

-

A

-

H

-

R

-

F

M

E

K

G

A

P

P

R

T

R

K

I

I

G

E

Q

N

-

V

-

S

D

F

A

A

D

P

G

G

N

N

P

V

T

S

W

-

S

-

V

-

P

-

E

-

L

I

A

K

W

W

F

F

E

E

G

D

G

G

R

V

L

L

N

N

V

V

A

A

H

H

N

N

C

C

V

I

D

D

R

R

H

H

V

L

E

H

A

K

G

-

R

R

G

A

A

N

H

Q

V

T

A

A

L

I

Y

I

F

W

E

E

G

G

-

D

E

D

P

P

G

S

D

Q

R

S

L

R

V

H

Y

I

T

T

Y

Y

A

K

D

E

L

L

Q

H

R

D

E

E

V

V

S

C

R

R

A

M

A

A

N

N

A

I

L

L

L

R

A

T

L

R

G

N

V

V

E

K

R

K

G

G

D

D

R

R

V

V

V

T

I

I

Y

Y

L

L

P

P

V

M

I

I

P

P

E

E

T

A

V

A

V

Y

I

A

T

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

I

H

H

S

S

L

V

V

V

F

F

G

A

G

G

F

F

S

S

A

P

E

D

A

S

L

L

R

A

F

Q

R

R

V

I

E

N

D

D

T

C

G

Q

A

S

K

K

V

V

L

I

V

I

T

T

T

A

D

D

G

E

Q

G

H

L

R

R

R

G

G

G

A

K

V

K

V

V

P

P

V

L

K

K

A

A

V

N

A

V

D

D

E

A

A

A

C

L

S

A

G

K

D

A

N

D

A

G

I

V

E

D

H

W

V

V

V

V

V

V

V

V

R

K

R

R

T

T

S

G

R

-

P

-

G

G

R

K

S

I

D

D

L

M

P

-

R

-

G

-

T

-

R

-

G

-

T

-

H

-

S

-

K

-

E

-

E

-

D

-

A

-

V

-

P

-

W

-

T

N

R

P

G

T

R

R

D

D

V

L

W

W

W

Y

H

H

E

E

L

A

V

A

A

A

E

M

Q

V

P

T

D

T

V

E

H

C

E

P

A

V

E

E

A

H

F

M

D

H

A

A

E

E

S

D

P

P

L

L

F

F

I

I

I

L

Y

Y

T

T

S

S

G
|
G

T

S

T

T

G
|
G

R

Q

P

P

K
|
K

G

G

L

V

V

L

H

H

T

T

M

S

G

A

G

G

Y

Y

L

L

V

V

Q

Y

A

A

A

A

Y

M

T

T

H

H

A

Q

L

Y

L

V

F

F

D

D

L

Y

L

H

P

-

D

-

V

-

R

-

D

-

D

-

E

-

G

-

R

-

L

-

R

-

P

-

D

-

E

-

L

-

S

-

T

-

V

-

N

-

D

-

P

-

A

-

K

-

V

-

D

D

S

G

T

D

V

I

H

Y

W

W

C

C

T

T

A

A

D

D

L

V

A

G

W

W

V

V

T

T

A

G

H

H

T

S

Y

Y

E

I

I

L

Y

Y

G

G

P

P

L

L

V

A

N

N

G

G

V

A

S

T

E

T

V

L

I

M

Y

F

E

E

G

G

T

V

P

P

N

N

T

Y

P

P

H

D

W

N

G

S

R

R

H

F

F

W

E

N

V

V

I

I

E

D

R

K

Y

H

G

K

V

V

T

N

T

T

Y

F

Y

Y

T

T

A

A

P

P

T

T

L

A

I

I

R

R

S

A

L

L

M

M

G

Q

A

G

F

-

P

-

K

-

G

G

V

D

P

E

P

P

L

V

Y

K

N

K

L

T

N

S

-

R

-

A

S

S

V

L

R

R

L

L

L

L

G

G

S

S

V

V

G

G

E

E

S

P

V

I

N

N

P

P

E

E

A

A

W

W

R

E

W

W

L

Y

R

H

A

R

Q

V

L

V

G

G

R

E

D

D

E

R

V

C

P

P

F

I

V

V

D

D

T

T

W

W

W

W

Q

Q

S

T

E
|
E

T

T

G

G

A

G

T

I

V

L

M

I

S

T

P

P

R

L

P

P

H

-

D

-

P

-

Q

-

F

-

A

-

P

-

P

-

G

-

T

-

F

-

D

-

D

-

G

G

A

A

P

-

H

-

T

T

A

K

L

L

K

K

P

P

G

G

C

S

A

A

T

T

R

Q

A

P

V

F

P

F

G

G

L

V

S

V

T

P

R

E

V

I

V

V

D

D

E

A

A

D

G

G

A

K

T

V

V

L

P

E

P

G

G

E

V

T

Q

T

G

G

F

N

V

L

V

C

V

L

D

T

R

R

T

A

G

W

P

P

S

G

M

M

A

M

R

R

T

T

V

V

W

Y

G

G

D

D

P

H

Q

A

R

R

Y

F

L

E

S

Q

S

T

Y

Y

W

F

Q

S

A

T

Y

Y

A

-

E

-

Q

K

G

G

W

K

F

Y

L

F

A

T

G

G

D

D

G

G

A

C

R

R

C

R

D

D

E

A

E

D

G

G

D

Y

I

Y

Y

W

I

I

L

T

G

G

R

R

V

V

D

D

D

D

V

V

I

I

N

N

V

V

S

S

G

G

H

H

R

R

L

M

S

G

T

T

I

A

E

E

I

V

E

E

S

S

A

A

L

L

V

V

T

A

H

H

P

E

W

K

V

V

V

S

E

E

A

A

G

A

A

V

A

V

P

G

V

F

R

P

D

H

E

D

L

I

T

K

G

G

H

Q

A

G

V

I

L

Y

A

A

Y

Y

V

V

V

T

L

L

T

M

P

A

E

G

A

V

E

Q

R

-

V

-

P

P

A

T

D

E

E

D

L

L

T

R

A

K

T

E

L

L

R

V

E

T

H

W

V

V

A

R

R

K

E

E

I

I

G

G

P

P

I

I

A

A

K

S

P

P

R

D

A

Q

V

I

V

Q

A

F

V

A

P

P

D

G

L

L

P

P

K

K

T

T

R

R

S

S</