SitesBLAST

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
33% identity, 76% coverage: 2:429/560 of query aligns to 11:430/629 of P30825

query
sites
P30825

S

Q

Q

Q

L

M

F

L

R

R

K

K

I

V

Y

V

S

D

D

C

T

S

D

R

T

E

S

E

T

T

G

R

L

L

L

S

R

R

V

C

L

L

G

N

V

T

W

F

D

D

I

L

V

V

F

A

F

L

G

G

I

V

A

G

A

S

I

T

I

L

G

G

A

A

G

G

S

V

F

Y

S

V

S

-

L

L

G

A

E

G

A

A

V

V

F

A

R

R

-

E

-

N

G

A

G

G

P

P

G

A

V

I

I

V

L

I

L

S

Y

F

L

L

I

I

C

A

G

A

F

L

A

A

C

S

G

V

F

L

T

A

A

G

L

L

C

C

Y

Y

A

G

E

E

F

F

A

G

S

A

R

R

I

V

P

P

T

K

A

T

G

G

S

S

A

A

Y

Y

T

L

Y

Y

A

S

Y

Y

A

V

S

T

F

V

G

G

E

E

L

L

I

W

A

A

W

F

I

I

I

T

G

G

W

W

A

N

L

L

I

I

M

L

E

S

Y

Y

S

I

F

I

G

G

N

T

I

S

Y

S

V

V

A

A

F

R

S

A

W

W

S

S

D

A

Y

T

F

F

T

D

S

E

F

L

L

I

G

G

R

R

L

-

G

-

M

-

H

P

I

I

P

G

D

E

Y

F

L

-

T

-

C

-

S

-

Y

-

T

-

E

-

A

-

R

-

K

-

A

-

V

-

Q

-

Y

-

G

-

S

S

E

R

N

T

K

H

E

M

L

T

L

L

N

N

A

A

W

-

N

-

T

-

A

-

P

-

L

-

I

P

G

G

S

V

L

L

K

A

F

E

I

N

V

P

D

D

I

I

P

F

A

A

L

V

V

I

I

I

N

I

G

L

L

I

I

L

T

T

W

G

L

L

C

L

Y

T

V

L

G

G

V

V

K

K

E

E

S

S

K

A

N

M

F

V

N

N

N

K

S

I

L

F

V

T

I

C

L

I

K

N

L

V

G

L

V

V

I

L

I

G

L

F

V

I

I

M

L

V

V

S

G

G

F

F

A

V

Y

K

I

G

N

S

T

V

D

K

N

N

W

W

T

Q

P

L

V

T

S

E

P

E

A

D

T

F

G

G

M
x
N

P

T

S

S

-

G

-

R

-

L

-

C

-

L

-

N

-

D

-

T

-

K

-

E

-

G

-

K

-

P

-

G

-

V

-

G

F

F

M

M

P

P

N

F

G

G

F

F

A

S

G

G

V

V

M

L

S

S

A

G

V

A

S

A

G

T

V

C

F

F

F

Y

A

A

Y

F

I

V

G

G

F

F

D

D

A

C

L

I

S

A

V

T

L

T

S

G

E

E

T

V

K

K

D

N

P

P

Q

Q

K

K

T

A

L

I

P

P

K

V

G

G

M

I

I

V

I

A

S

S

L

L

V

L

L

I

C

C

T

F

V

I

I

A

Y

Y

I

F

A

G

L

V

T

S

L

A

V

A

L

L

T

T

G

L

M

M

V

M

D

P

Y

Y

K

F

K

C

F

L

D

D

G

N

V

-

G

N

D

S

P

P

L

L

S

P

F

D

I

A

F

F

E

K

K

-

T

-

N

-

A

-

N

H

V

V

A

G

W

W

-

E

-

G

M

A

E

K

L

Y

I

A

V

V

S

A

F

V

V

G

A

S

I

L

V

C

A

A

I

L

T

S

T

A

V

S

L

L

V

G

F

S

Q

M

M

F

G

P

Q

M

P

P

R

R

I

V

W

I

Y

Y

A

A

M

M

S

A

R

E

D

D

G

G

L

L

M

L

P

F

Q

K

R

F

F

L

Q

A

K

N

I

V

H

N

P

D

K

R

Y

T

K

K

T

T

P

P

S

I

Y

I

A

A

T

T

I

L

V

A

T

S

G

G

I

A

V

V

V

A

G

A

I

V

P

M

I

A

L

F

F

L

T

F

D

D

K

L

T

K

F

D

I

L

L

V

D

D

F

L

T

M

S

S

I

I

G

G

T

T

I

L

F

L

A

A

F

Y

V

S

L

L

V

V

C

A

A

A

G

C

V

V

L

L

V

V

L

L

N226 (≠ M240) modified: carbohydrate, N-linked (GlcNAc...) asparagine

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
24% identity, 73% coverage: 16:426/560 of query aligns to 15:381/461 of P76037

query
sites
P76037

S

K

T

T

G

R

L

L

L

R

R

K

V

S

L

L

G

K

V

L

W

W

D

Q

I

V

V

V

F

M

G

G

I

L

A

A

A

Y

I

L

I

T

G

P

A

M

G

T

S

V

F

F

S

D

S

T

L

F

G

G

E

I

A

V

V

S

F

G

R

I

G

S

G

D

P

G

G

H

V

V

I

P

L

A

L

S

Y

Y

L

L

I

L

C

A

G

L

F

A

A

G

C

V

G

L

F

F

T

T

A

A

L

I

C

S

Y

Y

A

G

E

K

F

L

A

V

S

R

R

Q

I

F

P

P

T

E

A

A

G

G

S

S

A

A

Y

Y

T

T

Y

Y

A

A

Y

Q

A

K

S

S

F

I

G

N

E

P

L

H

I

V

A

G

W

F

I

M

I

V

G

G

W

W

A

S

L

S

I

L

M

L

E

D

Y
|
Y

S

-

F

-

G

-

N

-

I

-

Y

-

V

-

A

-

F

-

S

-

W

-

S

-

D

-

Y

L

F

F

T

L

S

P

F

M

L

I

G

N

R

V

L

L

G

L

M

A

H

K

I

I

P

-

D

-

Y

Y

L

L

T

S

C

A

S

L

Y

F

T

P

E

E

A

-

R

-

K

-

A

-

V

-

Q

-

Y

-

G

-

S

-

E

-

N

-

K

-

E

-

L

-

N

-

A

-

W

-

N

-

T

V

A

P

P

P

L

W

I

V

G

W

S

V

L

V

K

T

F

F

I

V

V

-

D

-

I

-

P

-

A

-

L

-

V

-

I

-

N

-

G

A

L

I

I

L

T

T

W

A

L

A

C

N

Y

L

V

K

G

S

V

V

K

N

E

L

S

V

K

A

N

N

F

F

N

N

N

T

S

L

L

F

V

V

I

L

L

V

K

Q

L

I

G

S

V

I

I

M

I

V

L

V

V

F

I

I

L

F

V

L

G

V

F

V

A

Q

Y

G

I

L

N

H

T

K

D

G

N

E

-

G

-

V

-

G

-

T

-

V

W

W

T

S

P

L

V

Q

S

P

P

F

A

I

T

S

G

E

M

N

P

A

S

H

F

L

M

I

P

P

N

-

G

-

F

-

A

-

G

-

V

I

M

I

S

T

A

G

V

A

S

T

G

I

V

V

F

C

F

F

A

S

Y

F

I

L

G

G

F

F

D

D

A

A

L

V

S

T

V

T

L

L

S

S

E

E

T

T

K

P

D

D

P

A

Q

A

K

R

T

V

L

I

P

P

K

K

G

A

M

I

I

F

I

L

S

T

L

A

V

V

L

Y

C

G

T

G

V

V

I

I

Y

F

I

I

A

A

L

A

T

S

L

F

V

F

L

M

T

Q

G

L

M

F

V

F

-

P

D

D

Y

I

K

S

K

R

F

F

D

K

G

-

V

-

G

-

D

D

P

P

L

-

S

-

F

-

I

-

F

-

E

-

K

-

T

-

N

D

A

A

N

A

V

L

A

P

W

E

M

I

E

A

L

L

I

Y

V

V

S

G

-

G

-

K

-

L

F

F

V

Q

A

S

I

I

V

F

A

L

I

C

T

T

V

F

L

V

-

N

-

T

-

L

-

A

-

S

-

G

L

L

V

A

F

S

Q

H

M

A

G

S

Q

V

P

S

R

R

I

L

W

L

Y

Y

A

V

M

M

S

G

R

R

D

D

G

N

L

V

M

F

P

P

Q

E

R

R

-

V

F

F

Q

G

K

Y

I

V

H

H

P

P

K

K

Y

W

K

R

T

T

P

P

S

A

Y

L

A

N

T

V

I

I

V

M

T

V

G

G

I

I

V

-

V

V

G

A

I

L

P

S

I

A

L

L

F

F

T

F

D

D

K

L

T

V

F

T

I

A

L

T

D

A

F

L

T

I

S

N

I

F

G

G

T

A

I

L

F

V

A

A

F

F

V

T

L

F

V

V

C

N

A

L

G

S

V

V

Y110 (= Y111) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

7b00A Human lat2-4f2hc complex in the apo-state (see paper)
23% identity, 84% coverage: 33:502/560 of query aligns to 10:455/457 of 7b00A

query
sites
7b00A

I

V

A

G

A

N

I

I

G

G

A

S

G

G

S

I

F

F

-

V

S

S

S

P

L

K

G

G

E

V

A

L

V

E

F

N

R

A

G

G

G

S

P

V

G

G

V

L

I

A

L

L

L

I

-

V

Y

W

L

I

I

V

C

T

G

G

F

F

A

I

C

T

G

V

F

V

T

G

A

A

L

L

C

C

Y

Y

A

A

E

E

F

L

A

G

S

V

R

T

I

I

P

P

T

K

A

S

G

G

S

G

A

D

Y

Y

T

S

Y

Y

A

V

Y

K

A

D

S

I

F

F

G

G

E

G

L

L

I

A

A

G

W

F

I

L

I

R

G

L

W

W

-

I

A

A

L

V

I

L

M

V

E

I

Y

Y

S

P

F

T

G

N

N

Q

I

A

Y

V

V

I

A

A

F

L

S

T

W

F

S

S

D

N

Y

Y

F

V

T

L

S

Q

F

-

L

-

G

-

R

-

L

-

G

-

M

-

H

-

I

-

P

P

D

L

Y

F

L

P

T

T

C

C

S

F

Y

P

T

P

E

E

A

S

R

G

K

L

A

R

V

L

Q

L

Y

-

G

-

S

-

E

-

N

-

K

-

E

-

L

-

N

-

A

-

W

-

N

-

T

-

A

-

P

-

L

-

I

-

G

-

S

-

L

-

K

-

F

-

I

-

V

-

D

-

I

-

P

-

A

A

L

A

V

I

I

C

N

L

G

L

L

L

I

L

T

T

W

W

L

V

C

N

Y

C

V

S

G

S

V

V

K

R

E

W

S

A

K

T

N

R

F

V

N

Q

N

D

S

I

L

F

V

T

I

A

L

G

K

K

L

L

G

L

V

A

I

L

I

A

L

L

V

I

I

I

L

I

V

M

G

G

F

I

A

V

Y

Q

I

I

N

C

T

K

D

G

N

E

W

Y

T

F

P

W

V

L

S

E

P

P

A

K

T

N

G

A

M

F

P

E

S

N

F

F

M

Q

P

E

N

P

G

D

F

I

A

G

G

L

V

V

M

A

S

L

A

A

V

F

S

L

G

Q

V

G

F

S

F

F

A

A

Y

Y

I

G

G

G

F

W

D

N

A

F

L

L

S

N

V

Y

L

V

S

T

E

E

T

L

K

V

D

D

P

P

Q

Y

K

K

T

N

L

L

P

P

K

R

G

A

M

I

I

F

I

I

S

S

L

I

V

P

L

L

C

V

T

T

V

F

I

V

Y

Y

I

V

-

F

A

A

L

N

T

V

L

A

V

Y

L

V

T

T

G

A

M

M

V

S

D

P

Y

Q

K

E

K

L

F

L

D

A

G

S

V

N

G

A

D

V

P

A

L

V

S

T

F

F

I

-

F

G

E

E

K

K

T

L

N

L

A

G

N

V

V

M

A

A

W

W

-

I

M

M

E

P

L

I

I

S

V

V

S

A

F

L

V

S

A

T

I

F

V

G

A

G

I

V

T

N

T

G

V

S

L

L

L

F

V

T

F

-

Q

-

M

-

G

-

Q

S

P

S

R

R

I

L

W

F

Y

F

A

A

M

G

S

A

R

R

D

E

G

G

L

H

M

L

P

P

Q

S

R

V

F

L

Q

A

K

M

I

I

H

H

P

V

K

K

Y

R

K

C

T

T

P

P

S

I

Y

P

A

A

T

L

I

L

V

F

T

T

G

C

I

I

V

S

V

T

G

L

I

L

P
x
M

I

L

L

V

F

T

T

S

D

D

K

M

T

Y

F

T

I

L

L

I

D

N

F
x
Y

T

V

S

G

I

F

G

I

T

N

I

Y

F

L

A

F

F

Y

V

G

L

V

V

T

C

V

A

A

G

G

V

Q

L

I

V

V

L

L

P

R

A

W

K

K

E

K

K

P

I

D

K

I

G

P

R

R

F

-

H

-

L

-

P

-

Y

P

V

I

N

K

G

I

K

N

I

L

F

F

P

P

V

I

I

I

F

Y

I

L

-

L

-

F

-

W

G

A

S

F

L

L

I

L

A

V

F

F

Y

S

Y

L

W

W

Q

S

-

E

-

P

-

V

-
x
V

-

C

-

G

-
x
I

-

G

-

L

-

A

-

I

-

M

-

L

-

T

-

G

-

V

P

P

E

V

F

Y

F

F

Q

L

N

G

L

V

M

Y

D

W

W

Q

S

H

D

K

P

P

K

K

E

-

G

-

E

C

F

F

R

S

A

D

S

F

I

I

F

E

F

L

I

T

L

L

I

V

N

S

L

Q

I

K

M

M

C

C

V

V

T

V

F

Y

binding Digitonin: M338 (≠ P398), Y350 (≠ F410), V408 (vs. gap), I411 (vs. gap)

7cmiB The lat2-4f2hc complex in complex with leucine (see paper)
23% identity, 84% coverage: 33:502/560 of query aligns to 10:455/458 of 7cmiB

query
sites
7cmiB

I

V

A

G

A

N

I
|
I

I

I

G
|
G

A

S

G
|
G

S

I

F

F

-

V

S

S

S

P

L

K

G

G

E

V

A

L

V

E

F

N

R

A

G

G

G

S

P

V

G

G

V

L

I

A

L

L

L

I

-

V

Y

W

L

I

I

V

C

T

G

G

F

F

A

I

C

T

G

V

F

V

T

G

A

A

L

L

C

C

Y

Y

A

A

E

E

F

L

A

G

S

V

R

T

I

I

P

P

T

K

A

S

G

G

S

G

A

D

Y

Y

T

S

Y

Y

A

V

Y

K

A

D

S

I

F

F

G

G

E

G

L

L

I

A

A

G

W

F

I

L

I

R

G

L

W

W

-

I

A

A

L

V

I

L

M

V

E

I

Y

Y

S

P

F

T

G
x
N

N

Q

I

A

Y

V

V

I

A

A

F

L

S

T

W

F

S

S

D

N

Y

Y

F

V

T

L

S

Q

F

-

L

-

G

-

R

-

L

-

G

-

M

-

H

-

I

-

P

P

D

L

Y

F

L

P

T

T

C

C

S

F

Y

P

T

P

E

E

A

S

R

G

K

L

A

R

V

L

Q

L

Y

-

G

-

S

-

E

-

N

-

K

-

E

-

L

-

N

-

A

-

W

-

N

-

T

-

A

-

P

-

L

-

I

-

G

-

S

-

L

-

K

-

F

-

I

-

V

-

D

-

I

-

P

-

A

A

L

A

V

I

I

C

N

L

G

L

L

L

I

L

T

T

W

W

L

V

C

N

Y

C

V

S

G

S

V

V

K

R

E

W

S

A

K

T

N

R

F

V

N

Q

N

D

S

I

L

F

V

T

I

A

L

G

K

K

L

L

G

L

V

A

I

L

I

A

L

L

V

I

I

I

L

I

V

M

G

G

F

I

A

V

Y

Q

I

I

N

C

T

K

D

G

N

E

W

Y

T

F

P

W

V

L

S

E

P

P

A

K

T

N

G

A

M

F

P

E

S

N

F

F

M

Q

P

E

N

P

G

D

F

I

A

G

G

L

V

V

M

A

S

L

A

A

V

F

S

L

G

Q

V

G

F

S

F
|
F

A

A

Y

Y

I
x
G

G

G

F

W

D

N

A

F

L

L

S

N

V

Y

L

V

S

T

E

E

T

L

K

V

D

D

P

P

Q

Y

K

K

T

N

L

L

P

P

K

R

G

A

M

I

I

F

I

I

S

S

L

I

V

P

L

L

C

V

T

T

V

F

I

V

Y

Y

I

V

-

F

A

A

L

N

T

V

L

A

V

Y

L

V

T

T

G

A

M

M

V

S

D

P

Y

Q

K

E

K

L

F

L

D

A

G

S

V

N

G

A

D

V

P

A

L

V

S

T

F

F

I

-

F

G

E

E

K

K

T

L

N

L

A

G

N

V

V

M

A

A

W

W

-

I

M

M

E

P

L

I

I

S

V

V

S

A

F

L

V

S

A

T

I

F

V

G

A

G

I

V

T

N

T

G

V

S

L

L

L

F

V

T

F

-

Q

-

M

-

G

-

Q

S

P

S

R

R

I

L

W

F

Y

F

A

A

M

G

S

A

R

R

D

E

G

G

L

H

M

L

P

P

Q

S

R

V

F

L

Q

A

K

M

I

I

H

H

P

V

K

K

Y

R

K

C

T

T

P

P

S

I

Y

P

A

A

T

L

I

L

V

F

T

T

G

C

I

I

V

S

V

T

G

L

I

L

P

M

I

L

L

V

F

T

T

S

D

D

K

M

T

Y

F

T

I

L

L

I

D

N

F

Y

T

V

S

G

I

F

G

I

T

N

I

Y

F

L

A

F

F

Y

V

G

L

V

V

T

C

V

A

A

G

G

V

Q

L

I

V

V

L

L

P

R

A

W

K

K

E

K

K

P

I

D

K

I

G

P

R

R

F

-

H

-

L

-

P

-

Y

P

V

I

N

K

G

I

K

N

I

L

F

F

P

P

V

I

I

I

F

Y

I

L

-

L

-

F

-

W

G

A

S

F

L

L

I

L

A

V

F

F

Y

S

Y

L

W

W

Q

S

-

E

-

P

-

V

-

C

-

G

-

I

-

G

-

L

-

A

-

I

-

M

-

L

-

T

-

G

-

V

P

P

E

V

F

Y

F

F

Q

L

N

G

L

V

M

Y

D

W

W

Q

S

H

D

K

P

P

K

K

E

-

G

-

E

C

F

F

R

S

A

D

S

F

I

I

F

E

F

L

I

T

L

L

I

V

N

S

L

Q

I

K

M

M

C

C

V

V

T

V

F

Y

binding leucine: I13 (= I36), G15 (= G38), G17 (= G40), N94 (≠ G114), F203 (= F260), G206 (≠ I263)

7cmhB The lat2-4f2hc complex in complex with tryptophan (see paper)
23% identity, 84% coverage: 33:502/560 of query aligns to 10:455/458 of 7cmhB

query
sites
7cmhB

I

V

A

G

A

N

I

I

G
|
G

A

S

G
|
G

S

I

F

F

-

V

S

S

S

P

L

K

G

G

E

V

A

L

V

E

F

N

R

A

G

G

G

S

P

V

G

G

V

L

I

A

L

L

L

I

-

V

Y

W

L

I

I

V

C

T

G

G

F

F

A

I

C

T

G

V

F

V

T

G

A

A

L

L

C

C

Y

Y

A

A

E

E

F

L

A

G

S

V

R

T

I

I

P

P

T

K

A

S

G

G

S

G

A

D

Y

Y

T

S

Y

Y

A

V

Y

K

A

D

S

I

F

F

G

G

E

G

L

L

I

A

A

G

W

F

I

L

I

R

G

L

W

W

-

I

A

A

L

V

I

L

M

V

E

I

Y

Y

S

P

F

T

G
x
N

N

Q

I

A

Y

V

V

I

A

A

F

L

S

T

W

F

S

S

D

N

Y

Y

F

V

T

L

S

Q

F

-

L

-

G

-

R

-

L

-

G

-

M

-

H

-

I

-

P

P

D

L

Y

F

L

P

T

T

C

C

S

F

Y

P

T

P

E

E

A

S

R

G

K

L

A

R

V

L

Q

L

Y

-

G

-

S

-

E

-

N

-

K

-

E

-

L

-

N

-

A

-

W

-

N

-

T

-

A

-

P

-

L

-

I

-

G

-

S

-

L

-

K

-

F

-

I

-

V

-

D

-

I

-

P

-

A

A

L

A

V

I

I

C

N

L

G

L

L

L

I

L

T

T

W

W

L

V

C

N

Y

C

V

S

G

S

V

V

K

R

E

W

S

A

K

T

N

R

F

V

N

Q

N

D

S

I

L

F

V

T

I

A

L

G

K

K

L

L

G

L

V

A

I

L

I

A

L

L

V

I

I

I

L

I

V

M

G

G

F

I

A

V

Y

Q

I

I

N

C

T

K

D

G

N

E

W

Y

T

F

P

W

V

L

S

E

P

P

A

K

T

N

G

A

M

F

P

E

S

N

F

F

M

Q

P

E

N

P

G

D

F

I

A

G

G

L

V

V

M

A

S

L

A

A

V

F

S

L

G

Q

V

G

F

S

F
|
F

A
|
A

Y

Y

I
x
G

G

G

F

W

D

N

A

F

L

L

S

N

V

Y

L

V

S

T

E

E

T

L

K

V

D

D

P

P

Q

Y

K

K

T

N

L

L

P

P

K

R

G

A

M

I

I

F

I

I

S

S

L

I

V

P

L

L

C

V

T

T

V

F

I

V

Y

Y

I

V

-

F

A

A

L

N

T

V

L

A

V

Y

L

V

T

T

G

A

M

M

V

S

D

P

Y

Q

K

E

K

L

F

L

D

A

G

S

V

N

G

A

D

V

P

A

L

V

S

T

F

F

I

-

F

G

E

E

K

K

T

L

N

L

A

G

N

V

V

M

A

A

W

W

-

I

M

M

E

P

L

I

I

S

V

V

S

A

F

L

V

S

A

T

I

F

V

G

A

G

I

V

T

N

T

G

V

S

L

L

L

F

V

T

F

-

Q

-

M

-

G

-

Q

S

P

S

R

R

I

L

W

F

Y

F

A

A

M

G

S

A

R

R

D

E

G

G

L

H

M

L

P

P

Q

S

R

V

F

L

Q

A

K

M

I

I

H

H

P

V

K

K

Y

R

K

C

T

T

P

P

S

I

Y

P

A

A

T

L

I

L

V

F

T

T

G

C

I

I

V

S

V

T

G

L

I

L

P

M

I

L

L

V

F

T

T

S

D

D

K

M

T

Y

F

T

I

L

L

I

D

N

F

Y

T

V

S

G

I

F

G

I

T
x
N

I

Y

F

L

A

F

F

Y

V

G

L

V

V

T

C

V

A

A

G

G

V

Q

L

I

V

V

L

L

P

R

A

W

K

K

E

K

K

P

I

D

K

I

G

P

R

R

F

-

H

-

L

-

P

-

Y

P

V

I

N

K

G

I

K

N

I

L

F

F

P

P

V

I

I

I

F

Y

I

L

-

L

-

F

-

W

G

A

S

F

L

L

I

L

A

V

F

F

Y

S

Y

L

W

W

Q

S

-

E

-

P

-

V

-

C

-

G

-

I

-

G

-

L

-

A

-

I

-

M

-

L

-

T

-

G

-

V

P

P

E

V

F

Y

F

F

Q

L

N

G

L

V

M

Y

D

W

W

Q

S

H

D

K

P

P

K

K

E

-

G

-

E

C

F

F

R

S

A

D

S

F

I

I

F

E

F

L

I

T

L

L

I

V

N

S

L

Q

I

K

M

M

C

C

V

V

T

V

F

Y

binding tryptophan: G15 (= G38), G17 (= G40), N94 (≠ G114), F203 (= F260), A204 (= A261), G206 (≠ I263), N355 (≠ T415)

Q9UHI5 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8 from Homo sapiens (Human) (see 3 papers)
23% identity, 84% coverage: 33:502/560 of query aligns to 50:495/535 of Q9UHI5

query
sites
Q9UHI5

I

V

A

G

A

N

I
|
I

I

I

G

G

A

S

G

G

S

I

F

F

-

V

S

S

S

P

L

K

G

G

E

V

A

L

V

E

F

N

R

A

G

G

G

S

P

V

G

G

V

L

I

A

L

L

L

I

-

V

Y

W

L

I

I

V

C

T

G

G

F

F

A

I

C

T

G

V

F

V

T

G

A

A

L

L

C

C

Y
|
Y

A

A

E

E

F

L

A

G

S

V

R

T

I

I

P

P

T

K

A

S

G

G

S

G

A

D

Y

Y

T

S

Y

Y

A

V

Y

K

A

D

S

I

F

F

G

G

E

G

L

L

I

A

A

G

W

F

I

L

I

R

G

L

W

W

-

I

A

A

L

V

I

L

M

V

E

I

Y

Y

S

P

F

T

G
x
N

N

Q

I

A

Y

V

V

I

A

A

F

L

S

T

W

F

S

S

D

N

Y

Y

F

V

T

L

S

Q

F

-

L

-

G

-

R

-

L

-

G

-

M

-

H

-

I

-

P

P

D

L

Y

F

L

P

T

T

C
|
C

S

F

Y

P

T

P

E

E

A

S

R

G

K

L

A

R

V

L

Q

L

Y

-

G

-

S

-

E

-

N

-

K

-

E

-

L

-

N

-

A

-

W

-

N

-

T

-

A

-

P

-

L

-

I

-

G

-

S

-

L

-

K

-

F

-

I

-

V

-

D

-

I

-

P

-

A

A

L

A

V

I

I

C

N

L

G

L

L

L

I

L

T

T

W
|
W

L

V

C

N

Y

C

V

S

G

S

V

V

K

R

E

W

S

A

K

T

N

R

F

V

N

Q

N

D

S

I

L

F

V

T

I

A

L

G

K

K

L

L

G

L

V

A

I

L

I

A

L

L

V

I

I

I

L

I

V

M

G

G

F

I

A

V

Y

Q

I

I

N

C

T

K

D

G

N

E

W

Y

T

F

P

W

V

L

S

E

P

P

A

K

T

N

G

A

M

F

P

E

S

N

F

F

M

Q

P

E

N

P

G

D

F

I

A

G

G

L

V

V

M

A

S

L

A

A

V

F

S

L

G

Q

V

G

F

S

F
|
F

A

A

Y

Y

I
x
G

G

G

F

W

D

N

A

F

L

L

S

N

V

Y

L

V

S

T

E

E

T

L

K

V

D

D

P

P

Q

Y

K

K

T

N

L

L

P

P

K

R

G

A

M

I

I

F

I

I

S

S

L

I

V

P

L

L

C

V

T

T

V

F

I

V

Y

Y

I

V

-

F

A

A

L

N

T

V

L

A

V

Y

L

V

T

T

G

A

M

M

V

S

D

P

Y

Q

K

E

K

L

F

L

D

A

G

S

V

N

G

A

D
x
V

P

A

L

V

S

T

F

F

I

-

F

G

E

E

K

K

T

L

N

L

A

G

N

V

V

M

A

A

W

W

-

I

M

M

E

P

L

I

I

S

V

V

S

A

F

L

V

S

A

T

I

F

V

G

A

G

I

V

T

N

T

G

V

S

L

L

L

F

V

T

F

-

Q

-

M

-

G

-

Q

S

P

S

R

R

I

L

W

F

Y

F

A

A

M

G

S

A

R

R

D

E

G

G

L

H

M

L

P

P

Q

S

R

V

F

L

Q

A

K

M

I

I

H

H

P

V

K

K

Y

R

K

C

T

T

P

P

S

I

Y

P

A

A

T

L

I

L

V

F

T

T

G

C

I

I

V

S

V

T

G

L

I

L

P

M

I

L

L

V

F

T

T

S

D

D

K

M

T

Y

F

T

I

L

L

I

D

N

F

Y

T

V

S

G

I

F

G

I

T
x
N

I
x
Y

F

L

A

F

F

Y

V

G

L

V

V
x
T

C

V

A

A

G

G

V

Q

L

I

V

V

L

L

P

R

A

W

K

K

E

K

K

P

I

D

K

I

G

P

R
|
R

F

-

H

-

L

-

P

-

Y

P

V

I

N

K

G

I

K

N

I

L

F

F

P

P

V

I

I

I

F

Y

I

L

-

L

-

F

-

W

G

A

S

F

L

L

I

L

A

V

F

F

Y

S

Y

L

W

W

Q

S

-

E

-

P

-

V

-

C

-

G

-

I

-

G

-

L

-

A

-

I

-

M

-

L

-

T

-

G

-
x
V

P

P

E

V

F

Y

F

F

Q

L

N

G

L

V

M

Y

D

W

W

Q

S

H

D

K

P

P

K

K

E

-

G

-

E

C

F

F

R

S

A

D

S

F

I

I

F

E

F

L

I

T

L

L

I

V

N

S

L

Q

I

K

M

M

C

C

V

V

T

V

F

Y

I53 (= I36) binding L-leucine
Y93 (= Y74) mutation to A: Nearly complete reduction of glycine, L-alanine, and L-glutamine uptake. Minimal effect on the transport of L-isoleucine, L-histidine and L-tryptophan.
N134 (≠ G114) Important for substrate specificity; binding L-tryptophan; mutation to Q: Reduces L-leucine uptake activity. Abolishes L-tryptophan uptake.; mutation to S: The substrate specificity changed dramatically reducing L-glutamine, glycine and L-alanine uptake activity thus mimicking the selectivity of SLC7A5.
C154 (= C143) modified: Interchain (with C-210 in SLC3A2)
W174 (= W191) mutation to A: Does not affect protein expression, plasma membrane localization, or L-alanine uptake.
F243 (= F260) mutation to A: Abolishes leucine and tryptophan transport activities.
G246 (≠ I263) Important for substrate specificity; binding L-leucine; mutation to S: Strong decrease in the uptake of large substrates L-tryptophan, L-glutamine, and L-histidine but increases the uptake of small neutral amino acids glycine and L-alanine.
V302 (≠ D318) to I: found in a patient with age-related hearing loss; does not affect L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs142951280
N395 (≠ T415) binding L-tryptophan; mutation to Q: Strongly reduces L-leucine uptake activity. Strongly reduces L-tryptophan uptake activity.
Y396 (≠ I416) mutation to A: Strongly reduces L-leucine uptake activity.
T402 (≠ V422) to M: found in a patient with age-related hearing loss; strongly decreased L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs758342760
R418 (= R438) to C: found in a patient with age-related hearing loss; decreases L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs146946494
V460 (vs. gap) to E: found in a patient with age-related hearing loss; strongly decreases L-alanine transport activity. Decreases L-tyrosine transport activity. Decreases cell membrane localization; dbSNP:rs2048595742

Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
23% identity, 76% coverage: 33:455/560 of query aligns to 49:430/531 of Q9QXW9

query
sites
Q9QXW9

I

V

A

G

A

N

I

I

G

G

A

S

G

G

S

I

F

F

-

V

S

S

S

P

L

K

G

G

E

V

A

L

V

E

F

N

R

A

G

G

G

S

P

V

G

G

V

L

I

A

L

L

L

I

-

V

Y

W

L

I

I

V

C

T

G

G

F

I

A

I

C

T

G

A

F

V

T

G

A

A

L

L

C

C

Y

Y

A

A

E

E

F

L

A

G

S

V

R

T

I

I

P

P

T

K

A

S

G

G

S

G

A

D

Y

Y

T

S

Y

Y

A

V

Y

K

A

D

S

I

F

F

G

G

E

G

L

L

I

A

A

G

W

F

I

L

I

R

G

L

W

W

-

I

A

A

L

V

I

L

M

V

E

I

Y
|
Y

S

P

F

T

G
x
N

N

Q

I

A

Y

V

V

I

A

A

F

L

S

T

W

F

S

S

D

N

Y

Y

F

V

T

L

S

Q

F

-

L

-

G

-

R

-

L

-

G

-

M

-

H

-

I

-

P

P

D

L

Y

F

L

P

T

T

C

C

S

F

Y

P

T

P

E

E

A

S

R

G

K

L

A

R

V

L

Q

L

Y

-

G

-

S

-

E

-

N

-

K

-

E

-

L

-

N

-

A

-

W

-

N

-

T

-

A

-

P

-

L

-

I

-

G

-

S

-

L

-

K

-

F

-

I

-

V

-

D

-

I

-

P

-

A

A

L

A

V

I

I

C

N

L

G

L

L

L

I

L

T

T

W

W

L

V

C

N

Y

C

V

S

G

S

V

V

K

R

E

W

S

A

K

T

N

R

F

V

N

Q

N

D

S

I

L

F

V

T

I

A

L

G

K

K

L

L

G

L

V

A

I

L

I

A

L

L

V

I

I

I

L

I

V

M

G

G

F

I

A

V

Y

Q

I

I

N

C

T

K

D

G

N

E

W

F

T

F

P

W

V

L

S

E

P

P

A

K

T

N

G

A

M

F

P

E

S

N

F

F

M

Q

P

E

N

P

G

D

F

I

A

G

G

L

V

V

M

A

S

L

A

A

V

F

S

L

G

Q

V

G

F

S

F
|
F

A

A

Y

Y

I

G

G

G

F

W

D

N

A

F

L

L

S

N

V

Y

L

V

S

T

E

E

T

L

K

V

D

D

P

P

Q

Y

K

K

T

N

L

L

P

P

K

R

G

A

M

I

I

F

I

I

S

S

L

I

V

P

L

L

C

V

T

T

V

F

I

V

Y

Y

I

V

A

F

L

A

T

N

L

I

V

A

-

Y

L

V

T

T

G

A

M

M

V

S

D

P

Y

Q

K

E

K

L

F

L

D

A

G

S

V

N

G

A

D

V

P

A

L

V

S

T

F

F

I

-

F

G

E

E

K

K

T

L

N

L

A

G

N

V

V

M

A

A

W

W

-

I

M

M

E

P

L

I

I

S

V

V

S

A

F

L

V

S

A

T

I

F

V

G

A

G

I

V

T

N

T

G

V

S

L

L

L

F

V

T

F

-

Q

-

M

-

G

-

Q

S

P

S

R

R

I

L

W

F

Y

F

A

A

M

G

S

A

R

R

D

E

G

G

L

H

M

L

P

P

Q

S

R

V

F

L

Q

A

K

M

I

I

H

H

P

V

K

K

Y

R

K

C

T

T

P

P

S

I

Y

P

A

A

T

L

I

L

V

F

T

T

G

C

I

L

V

S

V

T

G

L

I

L

P

M

I

L

L

V

F

T

T

S

D

D

K

M

T

Y

F

T

I

L

L

I

D

N

F

Y

T

V

S

G

I

F

G

I

T

N

I

Y

F

L

A

F

F

Y

V

G

L

V

V

T

C

V

A

A

G

G

V

Q

L

I

V

V

L

L

P

R

A

W

K

K

E

K

K

P

I

D

K

I

G

P

R

R

F

-

H

-

L

-

P

-

Y

P

V

I

N

K

G

V

K

S

I

L

F

F

P

P

V

I

I

I

F

Y

I

L

Y130 (= Y111) mutation to A: Increases T2 import. Increases T3 and enables T4 import. Does not affect L-leucine and L-phenylalanine uptake.
N133 (≠ G114) mutation to S: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake. Increases the export of both L-leucine and L-phenylalanine.
F242 (= F260) mutation to W: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake.

P82251 b(0,+)-type amino acid transporter 1; b(0,+)AT1; Glycoprotein-associated amino acid transporter b0,+AT1; Solute carrier family 7 member 9 from Homo sapiens (Human) (see 11 papers)
23% identity, 78% coverage: 6:444/560 of query aligns to 13:412/487 of P82251

query
sites
P82251

R

E

R

K

K

S

I

I

Y

Q

S

S

D

Q

T

E

D

P

T

K

S

T

T

T

G

S

L

L

L

Q

R

K

V

E

L

L

G

G

V

L

W

I

D

S

I

G

V

I

F

S

F

I

G

I

I
x
V

A

G

A

T

I
|
I

G
|
G

A
x
S

G
|
G

S

I

F

F

S

V

S
|
S

L
x
P

G

K

-

S

-

V

-

L

-

S

-

N

-

T

E

E

A

A

V

V

F

-

R

-

G

-

G

G

P

P

G

C

V

L

I

I

L

I

L

-

Y

W

L
x
A

I

A

C

C

G

G

F

V

A

L

C

A

G

T

F

L

T

G

A

A

L

L

C

C

Y

F

A

A

E

E

F

L

A

G

S

T

R

M

I

I

P

T

T

K

A

S

G

G

S

G

A

E

Y

Y

T

P

Y
|
Y

A

L

Y

M

A

E

S

A

F

Y

G
|
G

E

P

L

I

I

P

A

A

W

Y

I

L

I

F

G

S

W
|
W

A

A

-

S

-

L

-

I

L

V

I
|
I

M

K

E

P

Y
x
T

S

S

F

F

G

A

N

I

I

I

Y

C

V

L

A

S

F

F

S

-

W

-

S

S

D

E

Y

Y

F

V

T

C

S

A

F

-

L

-

G

-

R

-

L

-

G

-

M

-

H

-

I

-

P

P

D

F

Y

Y

L
x
V

T

G

C
|
C

S

K

Y

P

T

P

E

Q

A

-

R

-

K

-

A

-

V

-

Q

-

Y

-

G

-

S

-

E

-

N

-

K

-

E

-

L

-

N

-

A

-

W

-

N

-

T

-

A

-

P

-

L

-

I

-

G

-

S

-

L

-

K

-

F

I

I

V

V

V

D

K

I

C

P

L

A

A

L

A

V

A

I

A

N

I

G

L

L

F

I

I

T

S

W

T

L

V

C

N

Y

S

V

L

G

S

V
|
V

K

R

E

L

S

G

K

S

N

Y

F

V

N

Q

N

N

S

I

L

F

V

T

I
x
A

L

A

K

K

L

L

G

V

V

I

I

V

I

A

L

I

V

I

I

I

L

I

V

S

G
|
G

F

L

A

V

Y

L

I

L

-

A

-

Q

-

G

N

N

T

T

D

K

N

N

W

F

T

D

P

-

V

-

S

-

P

-

A

-

T

-

G

-

M

-

P

-

S

-

F

-

M

-

P

-

N

N

G

S

F

F

A

E

G

G

V

A

M

Q

S

L

A

S

V

V

S

G

G

A

V

I

-

S

-
x
L

-
x
A

-

F

-

Y

-
x
N

-

G

F

L

F
x
W

A

A

Y

Y

I
x
D

G

G

F
x
W

D

N

A
x
Q

L

L

S

N

V

Y

L

I

S

T

E

E

T

L

K

R

D

N

P

P

Q

Y

K

R

T

N

L

L

P

P

K

L

G

A

M

I

I

I

S
x
G

L

I

V
x
P

L

L

C

V

T

T

V

A

I

C

Y

Y

I

I

A

L

L

M

T

N

L

V

V

S

-

Y

L

F

T

T

G

V

M

M

V

T

D

A

Y

T

K

E

K

L

F

L

D

Q

G
x
S

V

Q

G

A

D

V

P

A

L

V

S

T

F

F

I

-

F

G

E

D

K

R

T

V

N

L

A

Y

N

P

V

A

A

S

W

W

M

I

-

V

E

P

L

L

I

F

V

V

S

A

F

F

V

S

A

T

I

I

V

G

A

A

I

A

T

N

T

G

V

T

L

-

V
x
C

F

F

Q

T

M
x
A

G

G

Q

-

P

-

R

R

I

L

W

I

Y

Y

A
x
V

M
x
A

S

G

R
|
R

D

E

G

G

L

H

M

M

P

L

Q

K

R

V

F

L

Q

S

K

Y

I

I

H

S

P

V

K

R

Y

R

K

L

T

T

P

P

S

A

Y

P

A
|
A

T

I

I

I

V

F

T

Y

G

G

I

I

V

I

V

A

G

T

I

I

P

Y

I

I

L

I

F

P

T

G

D

D

K

I

T

N

F

S

I

L

L

V

D

N

F

Y

T

F

S
|
S

I

F

G

A

T
x
A

I
x
W

F

L

A

F

F
x
Y

V

G

L

L

V

T

C

I

A

L

G

G

V

L

L

I

V

V

L

M

P

R

-

F

A

T

K

R

E
x
K

K

E

I

L

K

E

G

R

R

P

F

I

H

K

L

V

P

P

Y

V

V

V

V40 (≠ I33) to M: in CSNU; uncertain significance
IIGSG 43:47 (≠ IIGAG 36:40) binding L-arginine
I44 (= I37) to T: in CSNU; type I; dbSNP:rs121908485
S51 (= S44) to F: in CSNU; uncertain significance
P52 (≠ L45) to L: in CSNU; impairs protein stability and dimer formation; dbSNP:rs1198613438
A70 (≠ L61) to V: in CSNU; partial loss of amino acid transport activity; dbSNP:rs769448665
Y99 (= Y90) to H: in CSNU; uncertain significance
G105 (= G96) to R: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908480
W114 (= W105) to R: in CSNU; uncertain significance
I120 (= I108) to L: in CSNU; uncertain significance
T123 (≠ Y111) to M: in CSNU; partial loss of amino acid transport activity; dbSNP:rs79987078
V142 (≠ L141) to A: no effect on amino acid transport activity; dbSNP:rs12150889
C144 (= C143) modified: Interchain (with C-114 in SLC3A1)
V170 (= V197) to M: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908479
A182 (≠ I209) to T: in CSNU; type III; partial loss of amino acid transport activity; dbSNP:rs79389353
G195 (= G222) to R: in CSNU; type III; decreased amino acid transport activity; dbSNP:rs121908482
L223 (vs. gap) to M: slightly decreased amino acid transport activity; dbSNP:rs1007160
A224 (vs. gap) to V: in CSNU; non-classic type I; dbSNP:rs140873167
N227 (vs. gap) to D: in CSNU; decreased amino acid transport activity
W230 (≠ F260) to R: in CSNU; complete loss of amino acid transport activity; mutation to A: Abolishes amino acid transport activity.
D233 (≠ I263) binding L-arginine; mutation to A: Complete loss of amino acid transport activity.
W235 (≠ F265) mutation to A: Complete loss of amino acid transport activity.
Q237 (≠ A267) mutation to A: Reduces amino acid transport activity.
G259 (≠ S289) to R: in CSNU; type III; impairs protein stability and dimer formation; dbSNP:rs121908483
P261 (≠ V291) to L: in CSNU; types I and III; dbSNP:rs121908486
S286 (≠ G315) to F: in CSNU; uncertain significance; dbSNP:rs755135545
C321 (≠ V352) mutation to S: Does not affect amino acid transport activity.
A324 (≠ M355) to E: in CSNU; uncertain significance
V330 (≠ A363) to M: in CSNU; type III; dbSNP:rs201618022
A331 (≠ M364) to V: in CSNU; non-classic type I; dbSNP:rs768466784
R333 (= R366) to Q: in CSNU; decreased amino acid transport activity; dbSNP:rs769576205; to W: in CSNU; severe loss of amino acid transport activity; dbSNP:rs121908484
A354 (= A387) to T: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs939028046
S379 (= S412) mutation to A: Markedly reduces amino acid transport activity.
A382 (≠ T415) to T: in CSNU; severe loss of amino acid transport activity; dbSNP:rs774878350
W383 (≠ I416) mutation to A: Complete loss of amino acid transport activity.
Y386 (≠ F419) mutation to A: Loss of amino acid transport activity.
K401 (≠ E433) to E: in CSNU; uncertain significance; dbSNP:rs760264924

Sites not aligning to the query:

426 L → P: in CSNU; uncertain significance
482 P → L: in CSNU; severe loss of amino acid transport activity; no effect on localization to the apical membrane; dbSNP:rs146815072; mutation P->A,G,S,V: No effect on amino acid transport activity.; mutation P->F,I,M,W: Decreased amino acid transport activity.

O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
23% identity, 66% coverage: 16:384/560 of query aligns to 5:328/438 of O34739

query
sites