SitesBLAST
Comparing WP_065399010.1 NCBI__GCF_001684965.1:WP_065399010.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8cqxA Ribokinase from t.Sp mutant a92g
32% identity, 95% coverage: 10:289/296 of query aligns to 2:289/300 of 8cqxA
- binding adenosine-5'-diphosphate: N179 (vs. gap), T217 (≠ R216), G219 (≠ D218), A220 (≠ T219), G222 (≠ S221), F250 (= F250), N272 (= N272), G275 (≠ S275), A276 (≠ S276), T279 (≠ S279)
- binding magnesium ion: D242 (≠ Y242), T244 (≠ M244), A278 (≠ V278), S287 (≠ G287)
6znxC Ribokinase from thermus species
31% identity, 95% coverage: 10:289/296 of query aligns to 2:254/265 of 6znxC
P0DX97 Deoxyribokinase; dRK; ATP:2-deoxy-D-ribose 5-phosphotransferase; EC 2.7.1.229 from Salmonella typhi (see paper)
28% identity, 96% coverage: 10:294/296 of query aligns to 3:298/306 of P0DX97
- M10 (≠ S17) Important for substrate specificity; mutation to N: 2.5-fold decrease in Vmax for deoxyribokinase activity and 70-fold increase in KM for deoxyribose. 2-fold increase in KM for ribose.
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 95% coverage: 8:289/296 of query aligns to 69:366/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
29% identity, 95% coverage: 8:289/296 of query aligns to 3:300/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (≠ K189), K225 (≠ R216), G227 (≠ D218), I246 (vs. gap), A248 (≠ K237), A257 (≠ M246), G258 (= G247), F261 (= F250), A286 (≠ S275), S287 (= S276)
- binding alpha-D-ribofuranose: N12 (≠ S17), D14 (= D19), G40 (= G45), K41 (= K46), N44 (= N49), E144 (= E145), D259 (= D248)
P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
27% identity, 95% coverage: 10:289/296 of query aligns to 7:296/309 of P0A9J6
1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
27% identity, 95% coverage: 10:289/296 of query aligns to 4:293/305 of 1rk2A
- active site: A249 (≠ G245), A250 (≠ M246), G251 (= G247), D252 (= D248)
- binding adenosine-5'-diphosphate: T220 (≠ R216), G222 (≠ D218), S223 (≠ T219), A250 (≠ M246), G251 (= G247), H276 (≠ N272), A279 (≠ S275)
- binding tetrafluoroaluminate ion: G213 (≠ Y209), R215 (≠ N211)
- binding magnesium ion: D246 (≠ E239), A282 (≠ V278), R285 (≠ G281), S291 (≠ G287)
- binding alpha-D-ribofuranose: N11 (≠ S17), D13 (= D19), G38 (= G44), G39 (= G45), K40 (= K46), N43 (= N49), E140 (= E145), D252 (= D248)
1gqtB Activation of ribokinase by monovalent cations (see paper)
27% identity, 95% coverage: 10:289/296 of query aligns to 6:295/307 of 1gqtB
- active site: A251 (≠ G245), A252 (≠ M246), G253 (= G247), D254 (= D248)
- binding phosphomethylphosphonic acid adenylate ester: N186 (≠ K189), T222 (≠ R216), G224 (≠ D218), S225 (≠ T219), A252 (≠ M246), G253 (= G247), H278 (≠ N272), A281 (≠ S275)
- binding cesium ion: D248 (≠ E239), I250 (≠ V241), A284 (≠ V278), R287 (≠ G281), S293 (≠ G287)
- binding alpha-D-ribofuranose: N13 (≠ S17), D15 (= D19), G41 (= G45), N45 (= N49), E142 (= E145), D254 (= D248)
6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
28% identity, 98% coverage: 6:294/296 of query aligns to 1:304/311 of 6wk0B
- binding phosphomethylphosphonic acid adenylate ester: N186 (≠ K189), T222 (≠ R216), G224 (≠ D218), A225 (≠ T219), G227 (≠ S221), T243 (≠ N235), V246 (≠ D238), A254 (≠ M246), G255 (= G247), N282 (= N272), A285 (≠ S275), A286 (≠ S276), V289 (≠ S279)
- binding alpha-D-ribofuranose: D14 (= D19), G40 (= G45), K41 (= K46), N44 (= N49), A96 (= A101), E141 (= E145), D256 (= D248)
5c41A Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
28% identity, 98% coverage: 6:294/296 of query aligns to 1:304/317 of 5c41A
- active site: G253 (= G245), A254 (≠ M246), G255 (= G247), D256 (= D248)
- binding phosphomethylphosphonic acid adenylate ester: N186 (≠ K189), T222 (≠ R216), G224 (≠ D218), A225 (≠ T219), G227 (≠ S221), V246 (≠ D238), G255 (= G247), N282 (= N272), A285 (≠ S275), A286 (≠ S276)
6wjzA Crystal structure of human ribokinase in complex with ampcp
28% identity, 98% coverage: 6:294/296 of query aligns to 1:304/315 of 6wjzA
- binding phosphomethylphosphonic acid adenosyl ester: N186 (≠ K189), T222 (≠ R216), G224 (≠ D218), A225 (≠ T219), G227 (≠ S221), T243 (≠ N235), V246 (≠ D238), A254 (≠ M246), G255 (= G247), N282 (= N272), A285 (≠ S275), A286 (≠ S276), V289 (≠ S279)
2fv7A Crystal structure of human ribokinase
28% identity, 96% coverage: 10:294/296 of query aligns to 4:303/308 of 2fv7A
- active site: G252 (= G245), A253 (≠ M246), G254 (= G247), D255 (= D248)
- binding adenosine-5'-diphosphate: N185 (≠ K189), T221 (≠ R216), G223 (≠ D218), G226 (≠ S221), T242 (≠ N235), V245 (≠ D238), A253 (≠ M246), G254 (= G247), N281 (= N272), A284 (≠ S275), A285 (≠ S276), V288 (≠ S279)
5c3yA Structure of human ribokinase crystallized with amppnp
28% identity, 96% coverage: 10:294/296 of query aligns to 4:303/306 of 5c3yA
- active site: G252 (= G245), A253 (≠ M246), G254 (= G247), D255 (= D248)
- binding amp phosphoramidate: T221 (≠ R216), G223 (≠ D218), V245 (≠ D238), T250 (≠ K243), G254 (= G247), N281 (= N272), A284 (≠ S275), A285 (≠ S276)
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
33% identity, 64% coverage: 9:198/296 of query aligns to 3:193/306 of 4xckA
Sites not aligning to the query:
- active site: 249, 250, 251, 252
- binding adenosine-5'-diphosphate: 220, 222, 223, 242, 247, 250, 254, 276, 279, 283
- binding alpha-D-ribofuranose: 248, 252
5byfA Crystal structure of human ribokinase in complex with amp
27% identity, 95% coverage: 14:294/296 of query aligns to 10:305/313 of 5byfA
Q9H477 Ribokinase; RK; EC 2.7.1.15 from Homo sapiens (Human)
27% identity, 95% coverage: 14:294/296 of query aligns to 22:317/322 of Q9H477