SitesBLAST
Comparing WP_065399519.1 NCBI__GCF_001684965.1:WP_065399519.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q5KUG0 Fused isobutyryl-CoA mutase; EC 5.4.99.13; EC 3.6.5.- from Geobacillus kaustophilus (strain HTA426) (see paper)
60% identity, 100% coverage: 6:1115/1115 of query aligns to 5:1086/1086 of Q5KUG0
- K213 (= K209) mutation to A: Loss of GTPase and ATPase activities. No effect on the mutase activity.
Q1LRY0 Fused isobutyryl-CoA mutase; EC 5.4.99.13; EC 3.6.5.- from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) (Ralstonia metallidurans) (see 2 papers)
58% identity, 99% coverage: 8:1115/1115 of query aligns to 23:1093/1093 of Q1LRY0
- H39 (= H24) binding axial binding residue
- 169:417 (vs. 155:403, 54% identical) GTPase chaperone MeaI
- GAGKSS 219:224 (= GAGKSS 206:211) binding GTP
- S223 (= S210) binding Mg(2+)
- I248 (= I234) binding Mg(2+)
- D249 (= D235) binding Mg(2+)
- D262 (= D248) binding Mg(2+); binding Mg(2+)
- R265 (= R251) binding GTP
- E310 (= E296) binding Mg(2+); binding Mg(2+)
- T311 (= T297) binding Mg(2+)
- NKFD 357:360 (≠ NKSD 343:346) binding GTP
- 418:579 (vs. 404:557, 28% identical) Linker
- F587 (= F565) binding substrate
- F598 (= F576) mutation to A: Switches the substrate specificity and enhances the catalytic efficiency of the isovaleryl-CoA mutase over the native isobutyryl-CoA mutase activity about 4000-fold. Is even more susceptible to inactivation than wild-type during turnover.
- R622 (= R600) binding substrate
- R728 (= R750) binding substrate
- Y772 (= Y794) binding substrate
- S821 (= S843) binding substrate
- R856 (= R878) binding substrate
- K861 (= K883) binding substrate
- E973 (= E995) binding GTP
- N1092 (= N1114) binding GTP
8sslA Isobutyryl-coa mutase fused q341a in the presence of gtp (see paper)
58% identity, 99% coverage: 8:1115/1115 of query aligns to 2:1072/1072 of 8sslA
- binding guanosine-5'-diphosphate: G200 (= G208), K201 (= K209), S202 (= S210), D241 (= D248), R244 (= R251), K337 (= K344), D339 (= D346), Q374 (≠ K381), A375 (= A382), S376 (= S383), N1071 (= N1114)
- binding magnesium ion: K201 (= K209), D241 (= D248)
4xc6A Isobutyryl-coa mutase fused with bound adenosylcobalamin, gdp, and mg (holo-icmf/gdp) (see paper)
58% identity, 99% coverage: 8:1115/1115 of query aligns to 3:1067/1067 of 4xc6A