SitesBLAST
Comparing WP_065400596.1 NCBI__GCF_001684965.1:WP_065400596.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
43% identity, 100% coverage: 1:268/269 of query aligns to 7:265/267 of 3ay6B
- active site: G24 (= G18), S151 (= S154), Y164 (= Y167), K168 (= K171)
- binding beta-D-glucopyranose: E102 (≠ Q98), S151 (= S154), H153 (= H156), W158 (= W161), Y164 (= Y167), N202 (= N205), K205 (≠ A208)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G14), T23 (≠ S17), G24 (= G18), L25 (≠ I19), Y45 (≠ S39), D71 (= D67), V72 (= V68), N98 (= N94), A99 (= A95), G100 (= G96), V101 (≠ I97), M149 (≠ I152), S151 (= S154), Y164 (= Y167), K168 (= K171), P194 (= P197), G195 (= G198), M197 (≠ I200), T199 (= T202), P200 (= P203), I201 (= I204), N202 (= N205)
P40288 Glucose 1-dehydrogenase; EC 1.1.1.47 from Priestia megaterium (Bacillus megaterium) (see 2 papers)
43% identity, 100% coverage: 1:268/269 of query aligns to 1:259/261 of P40288
- 11:35 (vs. 11:35, 44% identical) binding NADP(+)
- E96 (≠ Q98) mutation E->A,G,K: Heat stable.
- D108 (≠ Q110) mutation to N: Heat stable.
- V112 (= V114) mutation to A: Heat stable.
- E133 (≠ R135) mutation to K: Heat stable.
- V183 (= V192) mutation to I: Heat stable.
- P194 (= P203) mutation to Q: Heat stable.
- E210 (≠ L219) mutation to K: Heat stable.
- Y217 (≠ R226) mutation to H: Heat stable.
- Q252 (≠ T261) mutation to L: Heat stable.
- Y253 (≠ F262) mutation to C: Heat stable.
- A258 (≠ T267) mutation to G: Heat stable.
1g6kA Crystal structure of glucose dehydrogenase mutant e96a complexed with NAD+
43% identity, 100% coverage: 1:268/269 of query aligns to 1:259/261 of 1g6kA
- active site: G18 (= G18), S145 (= S154), Y158 (= Y167), K162 (= K171)
- binding nicotinamide-adenine-dinucleotide: T17 (≠ S17), G18 (= G18), L19 (≠ I19), R39 (≠ S39), D65 (= D67), V66 (= V68), N92 (= N94), A93 (= A95), G94 (= G96), M143 (≠ I152), S145 (= S154), Y158 (= Y167), P188 (= P197), G189 (= G198), I191 (= I200), T193 (= T202)
8w0oA Gdh-105 crystal structure
42% identity, 100% coverage: 1:268/269 of query aligns to 1:259/259 of 8w0oA
- binding nicotinamide-adenine-dinucleotide: G14 (= G14), S17 (= S17), G18 (= G18), L19 (≠ I19), Y39 (≠ S39), Q43 (≠ E45), D65 (= D67), V66 (= V68), N92 (= N94), A93 (= A95), G94 (= G96), M143 (≠ I152), Y158 (= Y167), K162 (= K171), P188 (= P197), G189 (= G198), I191 (= I200), T193 (= T202)
1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
40% identity, 96% coverage: 3:260/269 of query aligns to 2:250/252 of 1vl8B
- active site: G17 (= G18), S143 (= S154), I154 (≠ H164), Y157 (= Y167), K161 (= K171)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G14), R16 (≠ S17), G17 (= G18), L18 (≠ I19), S37 (≠ E41), R38 (= R42), C63 (= C66), D64 (= D67), V65 (= V68), A91 (≠ N94), A92 (= A95), G93 (= G96), I94 (= I97), V114 (= V117), I141 (= I152), S143 (= S154), Y157 (= Y167), K161 (= K171), P187 (= P197), G188 (= G198), Y190 (≠ I200), T192 (= T202), M194 (≠ I204), T195 (≠ N205)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
42% identity, 93% coverage: 10:259/269 of query aligns to 7:244/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
42% identity, 93% coverage: 10:259/269 of query aligns to 4:237/239 of 3sj7A
- active site: G12 (= G18), S138 (= S154), Q148 (≠ H164), Y151 (= Y167), K155 (= K171)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), S10 (= S16), R11 (≠ S17), I13 (= I19), N31 (= N37), Y32 (≠ H38), A33 (≠ S39), G34 (≠ S40), S35 (= S43), A58 (≠ C66), N59 (≠ D67), V60 (= V68), N86 (= N94), A87 (= A95), T109 (≠ V117), S138 (= S154), Y151 (= Y167), K155 (= K171), P181 (= P197), G182 (= G198)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
39% identity, 96% coverage: 4:262/269 of query aligns to 3:247/247 of P73574
- A14 (= A15) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ A162) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K171) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ A199) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ W209) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
40% identity, 93% coverage: 9:259/269 of query aligns to 3:242/244 of 1edoA
- active site: G12 (= G18), S138 (= S154), Y151 (= Y167), K155 (= K171)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), S10 (= S16), R11 (≠ S17), I13 (= I19), N31 (= N37), Y32 (≠ H38), A33 (≠ S39), R34 (= R42), S35 (= S43), D59 (= D67), V60 (= V68), N86 (= N94), A87 (= A95), S138 (= S154), Y151 (= Y167), K155 (= K171), P181 (= P197), G182 (= G198), I184 (= I200), S186 (≠ T202), M188 (≠ I204)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
38% identity, 96% coverage: 3:259/269 of query aligns to 1:245/247 of 4jroC
- active site: G16 (= G18), S142 (= S154), Q152 (≠ H164), Y155 (= Y167), K159 (= K171)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (= S16), R15 (≠ S17), G16 (= G18), I17 (= I19), N35 (= N37), Y36 (≠ H38), N37 (≠ S39), G38 (≠ S40), S39 (= S43), N63 (≠ D67), V64 (= V68), N90 (= N94), A91 (= A95), I93 (= I97), I113 (≠ V117), S142 (= S154), Y155 (= Y167), K159 (= K171), P185 (= P197), I188 (= I200), T190 (= T202)
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
36% identity, 96% coverage: 3:259/269 of query aligns to 1:241/244 of P0A2C9
- M125 (≠ F133) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (= A241) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (= S242) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
36% identity, 96% coverage: 3:259/269 of query aligns to 1:241/244 of 6t77A
- active site: G16 (= G18), S138 (= S154), Y151 (= Y167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (= S16), R15 (≠ S17), T37 (≠ S39), L58 (≠ C66), N59 (≠ D67), V60 (= V68), A87 (= A95), G88 (= G96), I89 (= I97)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
37% identity, 96% coverage: 3:259/269 of query aligns to 4:245/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G14), R18 (≠ S17), G19 (= G18), I20 (= I19), D39 (≠ S39), R40 (≠ S40), C63 (= C66), I65 (≠ V68), N91 (= N94), G93 (= G96), I94 (= I97), V114 (= V117), Y155 (= Y167), K159 (= K171), I188 (= I200), T190 (= T202), T193 (≠ N205)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
35% identity, 97% coverage: 1:260/269 of query aligns to 1:253/255 of 5itvA
- active site: G18 (= G18), S141 (= S154), Y154 (= Y167), K158 (= K171)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S17 (= S17), G18 (= G18), I19 (= I19), D38 (≠ H38), I39 (≠ S39), T61 (≠ C66), I63 (≠ V68), N89 (= N94), G91 (= G96), T139 (≠ I152), S141 (= S154), Y154 (= Y167), K158 (= K171), P184 (= P197), G185 (= G198), I186 (≠ A199), I187 (= I200)
7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
35% identity, 95% coverage: 5:259/269 of query aligns to 3:251/256 of 7do7A
- active site: G16 (= G18), S146 (= S154), Y159 (= Y167)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), R15 (≠ S17), G16 (= G18), I17 (= I19), S37 (= S39), D66 (= D67), A67 (≠ V68), N93 (= N94), A94 (= A95), G95 (= G96), I96 (= I97), V144 (≠ I152), S145 (= S153), S146 (= S154), Y159 (= Y167), K163 (= K171), P189 (= P197), G190 (= G198), I192 (= I200), T194 (= T202), I196 (= I204)
- binding beta-L-rhamnopyranose: F99 (≠ D100), S146 (= S154), S148 (≠ H156), Q156 (≠ H164), Y159 (= Y167), N197 (= N205), D235 (= D243), M236 (≠ L244), R238 (≠ D246)
7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
35% identity, 95% coverage: 5:259/269 of query aligns to 3:251/256 of 7b81A