SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_065401015.1 NCBI__GCF_001684965.1:WP_065401015.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P22033 Methylmalonyl-CoA mutase, mitochondrial; MCM; Methylmalonyl-CoA isomerase; EC 5.4.99.2 from Homo sapiens (Human) (see 28 papers)
63% identity, 95% coverage: 27:701/707 of query aligns to 65:737/750 of P22033

query
sites
P22033

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I69 (≠ L31) to V: in MMAM; likely benign; dbSNP:rs115923556
P86 (= P50) to L: in MMAM; mut0 and mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769348060
G87 (= G51) to E: in MMAM; mut0; dbSNP:rs1554160986
R93 (= R57) to H: in MMAM; mut0; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918251
G94 (= G58) to R: in MMAM; mut0; dbSNP:rs727504022; to V: in MMAM; mut- and mut0; dbSNP:rs535411418
P95 (= P59) to R: in MMAM; mut0; dbSNP:rs190834116
YPTM 96:99 (≠ YSTM 60:63) binding malonyl-CoA
Y100 (= Y64) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309735
W105 (= W69) to R: in MMAM; mut0; dbSNP:rs121918249
TIRQY 106:110 (= TIRQY 70:74) binding malonyl-CoA
R108 (= R72) to C: in MMAM; mut0; dbSNP:rs121918257; to G: in MMAM; mut-; dbSNP:rs121918257; to H: in MMAM; mut0; dbSNP:rs483352778
Q109 (= Q73) to R: in MMAM; mut0; dbSNP:rs1461110052
G133 (= G97) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253828
A137 (= A101) to V: in MMAM; mut0; dbSNP:rs941483851
D139 (= D103) to N: in MMAM; uncertain significance; dbSNP:rs879253829
L140 (= L104) to P: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
A141 (= A105) to T: in MMAM; decreased protein expression; dbSNP:rs1554160730
H143 (= H107) to Y: in MMAM; mut0
G145 (= G109) to S: in MMAM; mut0; dbSNP:rs1237080100
S148 (= S112) to L: in MMAM; mut0; dbSNP:rs1300547552
D156 (= D120) to N: in MMAM; mut-
G158 (= G122) to V: in MMAM; mut0
G161 (= G125) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs2127420039; to V: in MMAM; decreased protein expression
F174 (= F138) to S: in MMAM; mut0; dbSNP:rs864309733
M186 (= M150) to V: in MMAM; mut-; dbSNP:rs148331800
T187 (= T151) to S: in MMAM; mut0; dbSNP:rs879253830
N189 (= N153) to I: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs200908035; to K: in MMAM; mut-; dbSNP:rs1561959114
A191 (= A155) to E: in MMAM; mut- and mut0; affects proper folding; reduced protein level; decreased methylmalonyl-CoA mutase activity; dbSNP:rs760782399
A197 (≠ S161) to E: in MMAM; mut0
G203 (≠ A167) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs778702777
E205 (= E169) natural variant: Missing (in MMAM; mut0; dbSNP:rs879253831)
G215 (= G179) to C: in MMAM; mut- and mut0; dbSNP:rs121918258; to S: in MMAM; mut0; dbSNP:rs121918258
TIQ 216:218 (= TIQ 180:182) binding malonyl-CoA
Q218 (= Q182) to H: in MMAM; mut0 and mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs1446389693
N219 (= N183) to Y: in MMAM; mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs121918256
R228 (= R192) binding malonyl-CoA; to Q: in MMAM; mut0; dbSNP:rs770810987
T230 (= T194) to I: in MMAM; mut-; dbSNP:rs879253833; to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253833
Y231 (= Y195) to N: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309736
K255 (= K219) binding malonyl-CoA
S262 (= S226) to N: in MMAM; mut0; dbSNP:rs1767683356
H265 (= H229) binding malonyl-CoA; to Y: in MMAM; mut-
E276 (= E240) to D: in MMAM; uncertain significance; mut-; dbSNP:rs12175488
L281 (= L245) to S: in MMAM; mut0; dbSNP:rs796052007
G284 (= G248) to E: in MMAM; mut0; dbSNP:rs879253835; to R: in MMAM; mut0; dbSNP:rs761477436
S288 (≠ V252) to P: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1179778233
G291 (= G255) to E: in MMAM; mut0
Q293 (≠ K257) to P: in MMAM; mut0; dbSNP:rs138374956
RLS 304:306 (= RLS 268:270) binding malonyl-CoA
L305 (= L269) to S: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554160246
S306 (= S270) to F: in MMAM; mut0; dbSNP:rs1085307929
W309 (= W273) to G: in MMAM; decreased protein expression; dbSNP:rs2127418704
G312 (= G276) to V: in MMAM; mut0; dbSNP:rs864309734
Y316 (≠ F280) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; no decreased affinity for adenosylcob(III)alamin; dbSNP:rs781474200
A324 (= A288) to T: in MMAM; mut-; dbSNP:rs780387525
R326 (= R290) to K: in MMAM; uncertain significance; dbSNP:rs758577372
L328 (≠ I292) to F: in MMAM; mut0; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs796052002; to P: in MMAM; mut0; dbSNP:rs965316043
S344 (= S307) to F: in MMAM; mut-; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin
L346 (≠ A309) natural variant: Missing (in MMAM; mut0)
L347 (= L310) to R: in MMAM; mut0; dbSNP:rs1026703654
H350 (= H313) to Y: in MMAM; mut0; dbSNP:rs1407914109
L358 (= L321) to P: in MMAM; mut0; dbSNP:rs1767612147
N366 (= N329) to S: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309737
R369 (= R332) to C: in MMAM; mut0; dbSNP:rs772552898; to H: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs564069299
T370 (= T333) to P: in MMAM; mut0; dbSNP:rs368790885
A377 (≠ S340) to E: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918250
Q383 (= Q346) to H: in MMAM; mut0; to P: in MMAM; mut0
H386 (= H349) to N: in MMAM; mut0; dbSNP:rs1554159937; to R: in MMAM; mut0; dbSNP:rs866933356
T387 (= T350) to I: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs1400022403
N388 (= N351) to H: in MMAM; mut0; dbSNP:rs766010704; to K: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253840
S389 (≠ A352) natural variant: Missing (in MMAM; mut0)
I412 (≠ L375) natural variant: Missing (in MMAM; mut0)
P424 (= P387) to L: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253842
G426 (= G389) to E: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs533755473; to R: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769922244
G427 (= G390) to D: in MMAM; mut0; dbSNP:rs753288303
G454 (= G417) to E: in MMAM; mut0
A499 (= A463) to T: in dbSNP:rs2229385
I505 (= I469) to T: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
Q514 (= Q478) to E: in MMAM; uncertain significance; to K: in MMAM; decreased protein expression; dbSNP:rs2127416800
L518 (= L482) to P: in MMAM; mut0; dbSNP:rs864309738
R532 (≠ Q496) to H: in dbSNP:rs1141321
A535 (≠ N499) to P: in MMAM; mut0; dbSNP:rs760183775
A552 (≠ C516) to V: in MMAM; uncertain significance; dbSNP:rs879253845
C560 (≠ V524) to Y: in MMAM; mut0; dbSNP:rs1238333040
T566 (≠ S530) to R: in MMAM; mut0; dbSNP:rs1767288356
F573 (= F537) to S: in MMAM; mut-; affects proper folding; no effect on protein abundance; no effect on methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs775593146
Y587 (= Y551) to C: in MMAM; mut-
I597 (≠ F561) to R: in MMAM; no changed in protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554158951
P615 (= P579) to L: in MMAM; mut0; affects proper folding; reduced strongly protein level; dbSNP:rs1554158777; to R: in MMAM; mut0; dbSNP:rs1554158777; to T: in MMAM; mut0; affects proper folding; reduced strongly protein level; loss of methylmalonyl-CoA mutase activity; dbSNP:rs1302409621
R616 (= R580) to C: in MMAM; mut0; dbSNP:rs765284825
L617 (≠ I581) to R: in MMAM; mut0; dbSNP:rs1554158775
K621 (= K585) to N: in MMAM; mut0
G623 (= G587) to R: in MMAM; mut0; dbSNP:rs121918254
Q624 (= Q588) to R: in MMAM; no effect on protein abundance; dbSNP:rs768521956
D625 (= D589) to G: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253847; to V: in MMAM; mut0; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity
G626 (= G590) to C: in MMAM; mut-; dbSNP:rs982110849
H627 (= H591) binding axial binding residue; to R: in MMAM; mut0; dbSNP:rs372486357
G630 (= G594) to E: in MMAM; mut0; dbSNP:rs143023066
V633 (= V597) to G: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs200055428
G637 (≠ A601) to E: in MMAM; mut-; to R: in MMAM; mut0; dbSNP:rs781501004
F638 (= F602) to I: in MMAM; mut0
D640 (= D604) to Y: in MMAM; mut0; dbSNP:rs865815395
G642 (= G606) to R: in MMAM; mut-; dbSNP:rs747897332
G648 (≠ A612) to D: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs766721811
V669 (≠ L633) to E: in MMAM; mut0; dbSNP:rs1360470463
I671 (≠ V635) to V: in dbSNP:rs8589
L674 (= L638) to F: in MMAM; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1164271240
H678 (= H642) to R: in MMAM; mut-; dbSNP:rs147094927
E684 (≠ Q648) natural variant: E -> EL (in MMAM; mut-)
L685 (≠ V649) to R: in MMAM; mut-; dbSNP:rs864309739
R694 (≠ A658) to L: in MMAM; mut-; decreased protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs756225782; to W: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs777758903
M700 (≠ V664) to K: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs140600746
G703 (= G667) to R: in MMAM; mut0; dbSNP:rs121918255
G717 (= G681) to V: in MMAM; mut-; no effect on protein abundance; interferes with the binding of the cofactor to the apoenzyme; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; decreased thermodynamic stability; dbSNP:rs121918252
G723 (= G687) to D: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs755077681

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion
7:750 natural variant: Missing (in MMAM; mut-)
50 binding malonyl-CoA
152:750 natural variant: Missing (in MMAM; mut0)
228:750 natural variant: Missing (in MMAM; mut0)

8dyjB Crystal structure of human methylmalonyl-coa mutase in complex with adp and cob(ii)alamin (see paper)
63% identity, 95% coverage: 27:701/707 of query aligns to 29:701/708 of 8dyjB

query
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binding adenosine-5'-diphosphate: Y74 (= Y74), T151 (= T151), R192 (= R192), Y228 (= Y228), H229 (= H229), F272 (= F272), Q316 (= Q315), N352 (= N351), E356 (= E355), L360 (= L359), P361 (= P360)
binding cobalamin: F102 (= F102), L104 (= L104), H107 (= H107), A124 (= A124), V191 (= V191), R192 (= R192), H229 (= H229), E232 (= E232), G319 (= G318), W320 (= W319), E356 (= E355), G359 (≠ A358), L360 (= L359), G590 (= G590), H591 (= H591), D592 (= D592), R593 (= R593), G594 (= G594), I598 (≠ V598), S636 (= S636), L638 (= L638), A640 (= A640), G666 (= G666), G667 (= G667), V668 (= V668), F686 (= F686), G687 (= G687), T690 (= T690)

2xiqA Crystal structure of human methylmalonyl-coa mutase in complex with adenosylcobalamin and malonyl-coa (see paper)
63% identity, 95% coverage: 27:701/707 of query aligns to 30:702/714 of 2xiqA

query
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I

G

G

M

M

N

N

H

F

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Y

M

M

E

E

I

I

A

A

K

K

M

M

R

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A

A

A

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A

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-

E

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M

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A

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x
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G

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W

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E

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Y

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A

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E

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x
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K

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D

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N

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G

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H

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D

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R

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A

A

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V

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x
I

A

A

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A

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F

A

A

D

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G

G

F

F

D

D

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D

D

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F

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T

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P

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E

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A

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I

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L

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A
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G
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V

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I

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P

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P

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Q

D

D

Y

Y

E

E

F

F

L

L

Y

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A

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N

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G

G

A

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F

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G

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G

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|
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N

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A

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D

active site: Y75 (= Y74), Y229 (= Y228), H230 (= H229), K586 (= K585), D590 (= D589), H592 (= H591)
binding cobalamin: Y75 (= Y74), L105 (= L104), H108 (= H107), A125 (= A124), R193 (= R192), E233 (= E232), G320 (= G318), W321 (= W319), E357 (= E355), G360 (≠ A358), L361 (= L359), G591 (= G590), H592 (= H591), D593 (= D592), R594 (= R593), G595 (= G594), I599 (≠ V598), G635 (= G634), S637 (= S636), L639 (= L638), A641 (= A640), G667 (= G666), G668 (= G667), F687 (= F686), G688 (= G687), T691 (= T690)
binding malonyl-coenzyme a: Y61 (= Y60), T63 (= T62), M64 (= M63), R68 (≠ K67), T71 (= T70), R73 (= R72), Y75 (= Y74), S150 (= S149), T152 (= T151), T181 (= T180), R193 (= R192), K220 (= K219), H230 (= H229), R269 (= R268), S271 (= S270), F273 (= F272), R313 (= R311), A314 (≠ T312), H315 (= H313), Q317 (= Q315), Q348 (= Q346)

P11653 Methylmalonyl-CoA mutase large subunit; MCM-alpha; EC 5.4.99.2 from Propionibacterium freudenreichii subsp. shermanii (see 4 papers)
64% identity, 94% coverage: 41:707/707 of query aligns to 56:726/728 of P11653

query
sites
P11653

K

K

D

D

E

M

S

D

L

W

T

L

Q

D

T

T

S

Y

P

A

G

G

I

I

E

P

P

P

Y

F

L

V

R

H

G

G

P

P

Y
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Y

S

A

T

T

M
|
M

Y

Y

V

A

Q

F

K
x
R

P

P

W

W

T
|
T

I

I

R
|
R

Q

Q

Y
|
Y

A

A

G

G

F

F

S

S

T

T

A

A

E

K

E

E

S

S

N

N

A

A

F

F

Y

Y

R

R

N

N

L

L

A

A

G

G

Q

Q

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K

G

G

L

L

S
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S

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V

A

A

F
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F

D

D

L

L

A

P

T

T

H

H

R

R

G

G

Y

Y

D

D

S

S

D

D

H

N

S

P

R

R

V

V

V

A

G

G

D

D

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V

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G

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A
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A

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G

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V

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A

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I

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D

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Y

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D

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E

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L

D

D

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Q

I

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S

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M

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T

M

M

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N

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G

A

A

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L

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K

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E

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I

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A

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K

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W

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E

A

A

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G

A

A

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A

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A

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S

F

F

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W

A

G

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I

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G

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F

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A

A

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H