SitesBLAST
Comparing WP_066053917.1 NCBI__GCF_002869505.1:WP_066053917.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
39% identity, 99% coverage: 4:463/466 of query aligns to 2:464/465 of 3pm9A
- active site: A149 (= A151), L159 (≠ M161)
- binding flavin-adenine dinucleotide: P69 (= P71), Q70 (≠ S72), G71 (= G73), G72 (= G74), N73 (≠ R75), T74 (= T76), G75 (= G77), L76 (= L78), G79 (= G81), Q80 (≠ A82), L91 (= L93), L133 (≠ F135), G134 (≠ A136), A135 (≠ S137), C139 (≠ S141), T140 (≠ Q142), G142 (= G144), G143 (= G145), S146 (= S148), T147 (= T149), A149 (= A151), G150 (= G152), E200 (= E202), G201 (= G203), I205 (≠ V207), I206 (≠ V208), E423 (= E422)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
34% identity, 96% coverage: 21:466/466 of query aligns to 25:466/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R327), T337 (≠ S331), K348 (= K341), Y379 (≠ F374), H381 (= H376), H388 (= H383), H423 (= H423)
- binding flavin-adenine dinucleotide: W39 (= W35), P75 (= P71), Q76 (≠ S72), G77 (= G73), G78 (= G74), N79 (≠ R75), T80 (= T76), G81 (= G77), M82 (≠ L78), G85 (= G81), S86 (≠ A82), L139 (≠ F135), G140 (≠ A136), A141 (≠ S137), C145 (≠ S141), G149 (= G145), N150 (= N146), A152 (≠ S148), T153 (= T149), G157 (= G153), G207 (= G203), I212 (≠ V208), E422 (= E422), N459 (= N459)
- binding zinc ion: H381 (= H376), H388 (= H383), E422 (= E422)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
34% identity, 96% coverage: 21:466/466 of query aligns to 25:466/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (= W35), P75 (= P71), G77 (= G73), G78 (= G74), N79 (≠ R75), T80 (= T76), G81 (= G77), G85 (= G81), S86 (≠ A82), L139 (≠ F135), G140 (≠ A136), A141 (≠ S137), C145 (≠ S141), H146 (≠ Q142), G148 (= G144), G149 (= G145), N150 (= N146), A152 (≠ S148), T153 (= T149), A155 (= A151), E206 (= E202), G207 (= G203), I211 (≠ V207), I212 (≠ V208), E422 (= E422), N459 (= N459)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R327), T337 (≠ S331), K348 (= K341), Y379 (≠ F374), H381 (= H376), H388 (= H383), H423 (= H423)
- binding zinc ion: H381 (= H376), H388 (= H383), E422 (= E422)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
34% identity, 96% coverage: 21:466/466 of query aligns to 25:466/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (= W35), P75 (= P71), G77 (= G73), G78 (= G74), N79 (≠ R75), T80 (= T76), G81 (= G77), G85 (= G81), S86 (≠ A82), L139 (≠ F135), G140 (≠ A136), A141 (≠ S137), C145 (≠ S141), H146 (≠ Q142), G149 (= G145), N150 (= N146), A152 (≠ S148), T153 (= T149), A155 (= A151), G157 (= G153), E206 (= E202), G207 (= G203), I211 (≠ V207), I212 (≠ V208), E422 (= E422), N459 (= N459)
- binding d-malate: M82 (≠ L78), R333 (= R327), T337 (≠ S331), K348 (= K341), Y379 (≠ F374), H381 (= H376), H388 (= H383), E422 (= E422), H423 (= H423)
- binding zinc ion: H381 (= H376), H388 (= H383), E422 (= E422)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
34% identity, 96% coverage: 21:466/466 of query aligns to 25:466/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R327), T337 (≠ S331), K348 (= K341), Y379 (≠ F374), H381 (= H376), H388 (= H383), N390 (= N385), H423 (= H423)
- binding flavin-adenine dinucleotide: W39 (= W35), P75 (= P71), G77 (= G73), G78 (= G74), N79 (≠ R75), T80 (= T76), G81 (= G77), M82 (≠ L78), G85 (= G81), S86 (≠ A82), L139 (≠ F135), G140 (≠ A136), A141 (≠ S137), C145 (≠ S141), G149 (= G145), N150 (= N146), A152 (≠ S148), T153 (= T149), A155 (= A151), G157 (= G153), G207 (= G203), I212 (≠ V208), E422 (= E422), H423 (= H423)
- binding zinc ion: H381 (= H376), H388 (= H383), E422 (= E422)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
34% identity, 96% coverage: 21:466/466 of query aligns to 26:467/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (= W35), P76 (= P71), G78 (= G73), G79 (= G74), N80 (≠ R75), T81 (= T76), G82 (= G77), M83 (≠ L78), G86 (= G81), S87 (≠ A82), L140 (≠ F135), A142 (≠ S137), C146 (≠ S141), H147 (≠ Q142), G150 (= G145), N151 (= N146), A153 (≠ S148), T154 (= T149), G208 (= G203), I212 (≠ V207), I213 (≠ V208), E423 (= E422), N460 (= N459)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
34% identity, 96% coverage: 21:466/466 of query aligns to 78:519/521 of Q8N465
- S109 (≠ I52) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V70) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G74) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V90) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L96) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ V115) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P132) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (≠ S148) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ G174) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G176) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ S316) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R327) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ S331) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ P339) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K341) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ E359) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ Y366) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H376) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (= G378) to V: slight reduction in catalytic activity
- N439 (= N381) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H383) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (= N385) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (≠ I386) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ K388) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E422) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H423) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G424) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 98% coverage: 4:462/466 of query aligns to 27:494/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
29% identity, 99% coverage: 3:463/466 of query aligns to 5:463/467 of 7qh2C