SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_066195582.1 NCBI__GCF_002835735.1:WP_066195582.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
39% identity, 89% coverage: 16:257/271 of query aligns to 24:265/265 of P07821

query
sites
P07821

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K50 (= K42) mutation to Q: Lack of activity.
D172 (= D164) mutation to E: Lack of activity.
E173 (= E165) mutation to A: Lack of activity.

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
32% identity, 87% coverage: 2:236/271 of query aligns to 9:248/257 of P0AAH0

query
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P0AAH0

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G48 (= G41) mutation to I: Loss of phosphate transport.
K49 (= K42) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
33% identity, 80% coverage: 14:231/271 of query aligns to 37:254/382 of 7ahhC

query
sites
7ahhC

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binding phosphoaminophosphonic acid-adenylate ester: T39 (≠ Q16), V40 (= V17), G41 (≠ I18), G62 (= G39), G64 (= G41), K65 (= K42), D187 (= D164), E188 (= E165)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
33% identity, 80% coverage: 14:231/271 of query aligns to 37:254/382 of 7aheC

query
sites
7aheC

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Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7ahdC Opua (e190q) occluded (see paper)
33% identity, 80% coverage: 14:231/271 of query aligns to 37:254/260 of 7ahdC

query
sites
7ahdC

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binding adenosine-5'-triphosphate: T39 (≠ Q16), S61 (≠ N38), G62 (= G39), G64 (= G41), K65 (= K42), S66 (= S43), T67 (= T44), Q111 (= Q77), K161 (≠ D138), Q162 (≠ A139), S164 (= S141), G166 (= G143), M167 (≠ Q144), Q188 (≠ E165), H221 (= H198)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

5x40A Structure of a cbio dimer bound with amppcp (see paper)
35% identity, 87% coverage: 4:240/271 of query aligns to 5:246/280 of 5x40A

query
sites
5x40A

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E

D

A

L

L

R

T

I

Y

A

A

Y
x
F

-

P

G

G

D

G

Q
x
V

V

K

I
x
A

V

L

K

D

D

D

L

L

N

S

M

L

L

A

I

V

P

P

D

K

G

G

K

E

I

S

T

L

A

A

I

I

I

L

G

G

P

P

N
|
N

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

K

L

T

H

V

L

A

N

R

G

I

T

I

L

Q

R

A

P

K

Q

S

S

G

G

S

R

V

V

F

L

L

L

D

G

G

G

K

T

Q

A

I

T

-

G

-

H

S

S

K

R

E

K

S

D

T

L

K

T

S

G

I

W

A

R

Q

R

K

R

M

V

A

G

I

L

L

V

P

L

Q
|
Q

S

-

P

-

D

D

A

A

P

D

E

D

G

Q

L

L

-

F

-

A

-

T

T

T

I

V

G

F

E

E

L

D

V

V

S

S

Y

F

G

G

R

-

F

-

P

P

Y

L

Q

N

R

L

G

G

M

L

G

S

K

E

L

A

N

E

D

A

K

R

D

A

R

R

N

-

V

-

I

V

E

E

W

E

A

A

L

L

K

A

A

A

T

L

G

S

I

I

S

S

D

D

F

L

K

R

D

D

R

R

E

P

V

T

D
x
H

A
x
M

L
|
L

S
|
S

G

G

G
|
G

Q
|
Q

R

K

Q

R

R

R

V

V

W

A

I

I

A

A

M

G

A

A

L

V

A

A

Q

M

E

R

T

P

D

E

I

V

I

L

L

L

D

D

E
x
Q

P

P

T

T

T

A

Y

G

L

L

D

D

L

L

C

A

H

G

Q

T

L

E

E

Q

V

L

L

L

E

T

L

L

K

R

E

G

L

L

N

R

V

A

L

-

E

A

N

G

R

M

T

T

I

L

V

V

M

F

V

S

I

T

H
|
H

D

D

L

V

N

E

H

L

A

A

R

A

F

L

A

A

D

D

H

R

L

V

I

A

A

L

M

F

R

R

A

T

G

G

S

R

I

V

V

L

K

A

S

E

G

G

N

A

G

A

K

E

E

A

V

V

M

L

T

S

-

D

-

R

-

A

-

T

-

L

-

A

K

K

P

V

V

A

L

L

R

R

E

P

V

P

F

L

N

V

I

I

D

D

binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y13), V18 (≠ Q16), A20 (≠ I18), N40 (= N38), G41 (= G39), G43 (= G41), K44 (= K42), S45 (= S43), T46 (= T44), Q88 (= Q84), H139 (≠ D138), M140 (≠ A139), L141 (= L140), S142 (= S141), G144 (= G143), Q145 (= Q144), Q166 (≠ E165), H198 (= H198)
binding magnesium ion: S45 (= S43), Q88 (= Q84)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
33% identity, 84% coverage: 4:230/271 of query aligns to 2:226/240 of 4ymuJ

query
sites
4ymuJ

L

I

F

F

T

V

E

N

D

D

L

V

R

Y

I

K

A

N

Y
x
F

G

G

D

S

Q

L

V

E

I
x
V

V

L

K

K

D

G

L

V

N

T

M

L

L

K

I

V

P

N

D

K

G

G

K

E

I

V

T

V

A

V

I

I

G

G

P

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

K

R

T

C

V

I

A

N

R

L

I

L

I

E

Q

E

A

P

K

T

S

K

G

G

S

E

V

V

F

F

L

I

D

D

G

G

K

V

Q

K

I

I

S

N

-

N

-

G

K

K

E

V

S

N

T

I

K

N

S

K

I

V

A

R

Q

Q

K

K

M

V

A

G

I

M

L

V

P

F

Q

Q

S

H

P

F

D

N

A

L

P

F

E

P

G

H

L

L

T

T

I

A

G

I

E

E

L

N

V

I

S

T

Y

L

G

A

R

-

F

-

P

P

Y

V

Q

K

R

-

G

-

M

V

G

K

K

K

L

M

N

N

D

K

K

K

D

E

R

A

N

E

V

E

I

L

E

A

W

V

A

D

L

L

K

L

A

A

-

K

T

V

G

G

I

L

S

L

D

D

F

K

K

K

D

D

R

Q

E

Y

V

P

D

I

A

K

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

V

L

W

A

I

I

A

A

M

R

A

A

L

L

A

A

Q

M

E

Q

T

P

D

E

I

V

I

M

L

L

L

F

D

D

E
|
E

P

P

T

T

T

S

Y

A

L

L

D

D

L

P

C

E

H

M

Q

V

L

K

E

E

V

V

L

L

E

N

L

V

L

M

K

K

E

Q

L

L

N

-

V

A

L

N

E

E

N

G

R

M

T

T

I

M

V

V

M

V

V

V

I

T

H
|
H

D

E

L

M

N

G

H

F

A

A

A

R

R

E

F

V

A

G

D

D

H

R

L

V

I

I

A

F

M

M

R

D

A

D

G

G

S

V

I

I

V

V

K

E

S

E

G

G

N

T

G

P

K

E

E

E

V

I

M

F

T

Y

K

R

binding adenosine-5'-triphosphate: F11 (≠ Y13), V16 (≠ I18), S36 (≠ N38), G37 (= G39), S38 (≠ C40), G39 (= G41), K40 (= K42), S41 (= S43), T42 (= T44), E162 (= E165), H194 (= H198)
binding magnesium ion: S41 (= S43), E162 (= E165)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
29% identity, 87% coverage: 1:236/271 of query aligns to 1:230/371 of P68187

query
sites
P68187

M

M

S

A

R

S

L

V

F

Q

T

L

E

Q

D

N

L

V

R

T

I

K

A

A

Y

W

G

G

D

E

Q

V

V

V

I

V

V

S

K

K

D

D

L

I

N

N

M

L

L

D

I

I

P

H

D

E

G

G

K

E

I

F

T

V

A

V

I

F

I

V

G

G

P

P

N

S

G

G

C

C

G

G

K

K

S

S

T

T

V

L

L

L

K

R

T

M

V

I

A

A

R

G

I

L

I

E

Q

T

A

I

K

T

S

S

G

G

S

D

V

L

F

F

L

I

D

G

G

E

K

K

Q

R

I

M

-

N

-

D

-

T

-

P

S

A

K

E

E

R

S

G

T

V

K

G

S

M

I

V

A

F

Q

Q

K

S

M

Y

A
|
A

I

L

L

Y

P

P

Q

H

S

-

P

-

D

-

A

-

P

-

E

-

G

-

L

L

T

S

I

V

G

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

L

F

-

P

-

Y

-

Q

-

R

-

G

-

M

-

G

-

K

K

L

L

N

A

D

G

K

A

D
x
K

R

K

N

E

V

V

I

I

E

N

W

Q

A

R

L
x
V

K

N

A

Q

T
x
V

G

A

-
x
E

-

V

-

L

-

Q

I

L

S
x
A

D

H

F

L

K

L

D

D

R

R

E

K

V

P

D

K

A

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

R

R

V

V

W

A

I

I

A

G

M

R

A

T

L

L

A

V

Q

A

E

E

T

P

D

S

I

V

I

F

L

L

D
|
D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

L

A

C

A

H

L

Q

R

L

V

E

Q

V

M

L

R

E

I

L

E

L

I

K

S

E

R

L

L

N

H

V

K

L

R

E

L

N

G

R

R

T

T

I

M

V

I

M

Y

V

V

I

T

H

H

D

D

L

Q

N

V

H

E

A

A

A

M

R

T

F

L

A

A

D

D

H

K

L

I

I

V

A

V

M

L

R

D

A

A

G

G

S

R

I

V

V

A

K

Q

S

V

G

G

N

K

G

P

K

L

E

E

V

L

M

Y

T

H

K

Y

P

P

V

A

L

D

R
|
R

E

F

V

V

A85 (= A80) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ D116) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ L124) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ T127) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (vs. gap) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ S130) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G143) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D164) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (= R234) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

Q5M243 Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA1; EC 7.-.-.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
29% identity, 83% coverage: 19:243/271 of query aligns to 20:231/276 of Q5M243

query
sites
Q5M243

V

L

K

N

D

D

L

V

N

S

M

F

L

H

I

V

P

K

D

H

G

G

K

E

I

W

T

L

A

S

I

I

I

V

G

G

P

H

N

N

G

G

C

S

G

G

K

K

S

S

T

T

V

T

L

A

K

R

T

L

V

I

A

G

R

G

I

L

Q

V

A

A

K

D

S

S

G

G

S

Q

V

I

F

I

L

V

D

D

G

G

K

Q

Q

E

I

L

S

T

K

E

E

E

S

T

T

V

K

W

S

D

I

I

A

R

Q

D

K

K

M

I

A

G

I

M

L

V

P

F

Q

Q

S

N

P

P

D

D

A

N

P
x
Q

-

F

E

V

G

G

L

A

T

T

I

V

G

E

E

D

L

D

V

V

S

A

Y

F

G

G

-

L

-

E

-

N

-

K

R

G

F

L

P

P

Y

Y

Q

K

R

E

G

M

M

V

G

S

K

R

L

V

N

Q

D

E

K

-

D

-

R

-

N

-

V

-

I

-

E

-

W

-

A

A

L

L

K

S

A

F

T

V

G

G

I

M

S

M

D

D

F

F

K

K

D

D

R

R

E

E

V

P

D

A

A

R

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

V

V

W

A

I

I

A

A

M

G

A

I

L

I

A

A

Q

M

E

R

T

P

D

S

I

I

I

L

L

I

L

L

D

D

E
|
E

P

A

T

T

T

S

Y

M

L

L

D

D

L

P

C

E

H

G

Q

R

L

Q

E

E

V

L

L

I

E

Q

L

Y

L

I

K

E

E

D

L

I

N

R

V

Q

L

Q

E

Y

N

G

R

M

T

T

I

V

V

L

M

S

V

I

I

T

H

H

D

D

L

L

N

D

H

E

A

V

A

A

R

-

F

M

A

S

D

N

H

R

L

V

I

L

A

V

M

L

R

K

A

Q

G

G

S

K

I

V

V

-

K

-

S

-

G

-

N

-

G

-

K

-

E

E

V

S

M

I

T

S

K

S

P

P

V

-

L

-

R

R

E

E

V

L

F

F

N

S

I

R

D

G

A

S

E

E

I

L

Q90 (≠ P89) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-97 in EcfA2.
E163 (= E165) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-171 in EcfA2.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
30% identity, 83% coverage: 12:236/271 of query aligns to 11:229/371 of 3puyA

query
sites
3puyA

A

A

Y
x
W

G

G

D

E

Q

V

V

V

I

V

V

S

K

K

D

D

L

I

N

N

M

L

L

D

I

I

P

H

D

E

G

G

K

E

I

F

T

V

A

V

I

F

I

V

G

G

P

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

K

R

T

M

V

I

A

A

R

G

I

L

I

E

Q

T

A

I

K

T

S

S

G

G

S

D

V

L

F

F

L

I

D

G

G

E

K

K

Q

R

I

M

-

N

-

D

-

T

-

P

S

A

K

E

E

R

S

G

T

V

K

G

S

M

I

V

A

F

Q
|
Q

K

S

M

Y

A

A

I

L

L

Y

P

P

Q

H

S

-

P

-

D

-

A

-

P

-

E

-

G

-

L

L

T

S

I

V

G

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

L

F

-

P

-

Y

-

Q

-

R

-

G

-

M

-

G

-

K

K

L

L

N

A

D

G

K

A

D

K

R

K

N

E

V

V

I

I

E

N

W

Q

A

R

L

V

K

N

A

Q

T

V

G

A

-

E

-

V

-

L

-

Q

I

L

S

A

D

H

F

L

K

L

D

D

R
|
R

E

K

V

P

D

K

A
|
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

W

A

I

I

A

G

M

R

A

T

L

L

A

V

Q

A

E

E

T

P

D

S

I

V

I

F

L

L

D

D

E
|
E

P

P

T

L

T

S

Y

N

L

L

D

D

L

A

C

A

H

L

Q

R

L

V

E

Q

V

M

L

R

E

I

L

E

L

I

K

S

E

R

L

L

N

H

V

K

L

R

E

L

N

G

R

R

T

T

I

M

V

I

M

Y

V

V

I

T

H
|
H

D

D

L

Q

N

V

H

E

A

A

A

M

R

T

F

L

A

A

D

D

H

K

L

I

I

V

A

V

M

L

R

D

A

A

G

G

S

R

I

V

V

A

K

Q

S

V

G

G

N

K

G

P

K

L

E

E

V

L

M

Y

T

H

K

Y

P

P

V

A

L

D

R

R

E

F

V

V

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y13), S37 (≠ N38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (= S43), T43 (= T44), Q81 (= Q77), R128 (= R135), A132 (= A139), S134 (= S141), G136 (= G143), Q137 (= Q144), E158 (= E165), H191 (= H198)
binding magnesium ion: S42 (= S43), Q81 (= Q77)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
30% identity, 83% coverage: 12:236/271 of query aligns to 11:229/371 of 3puxA

query
sites
3puxA

A

A

Y
x
W

G

G

D

E

Q

V

V

V

I

V

V

S

K

K

D

D

L

I

N

N

M

L

L

D

I

I

P

H

D

E

G

G

K

E

I

F

T

V

A

V

I

F

I

V

G

G

P

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

K

R

T

M

V

I

A

A

R

G

I

L

I

E

Q

T

A

I

K

T

S

S

G

G

S

D

V

L

F

F

L

I

D

G

G

E

K

K

Q

R

I

M

-

N

-

D

-

T

-

P

S

A

K

E

E

R

S

G

T

V

K

G

S

M

I

V

A

F

Q
|
Q

K

S

M

Y

A

A

I

L

L

Y

P

P

Q

H

S

-

P

-

D

-

A

-

P

-

E

-

G

-

L

L

T

S

I

V

G

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

L

F

-

P

-

Y

-

Q

-

R

-

G

-

M

-

G

-

K

K

L

L

N

A

D

G

K

A

D

K

R

K

N

E

V

V

I

I

E

N

W

Q

A

R

L

V

K

N

A

Q

T

V

G

A

-

E

-

V

-

L

-

Q

I

L

S

A

D

H

F

L

K

L

D

D

R
|
R

E

K

V

P

D

K

A

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

W

A

I

I

A

G

M

R

A

T

L

L

A

V

Q

A

E

E

T

P

D

S

I

V

I

F

L

L

D

D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

L

A

C

A

H

L

Q

R

L

V

E

Q

V

M

L

R

E

I

L

E

L

I

K

S

E

R

L

L

N

H

V

K

L

R

E

L

N

G

R

R

T

T

I

M

V

I

M

Y

V

V

I

T

H
|
H

D

D

L

Q

N

V

H

E

A

A

A

M

R

T

F

L

A

A

D

D

H

K

L

I

I

V

A

V

M

L

R

D

A

A

G

G

S

R

I

V

V

A

K

Q

S

V

G

G

N

K

G

P

K

L

E

E

V

L

M

Y

T

H

K

Y

P

P

V

A

L

D

R

R

E

F

V

V

binding adenosine-5'-diphosphate: W12 (≠ Y13), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (= S43), T43 (= T44), R128 (= R135), S134 (= S141), Q137 (= Q144)
binding beryllium trifluoride ion: S37 (≠ N38), G38 (= G39), K41 (= K42), Q81 (= Q77), S134 (= S141), G136 (= G143), H191 (= H198)
binding magnesium ion: S42 (= S43), Q81 (= Q77)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
30% identity, 83% coverage: 12:236/271 of query aligns to 11:229/371 of 3puwA

query
sites
3puwA

A

A

Y
x
W

G

G

D

E

Q

V

V

V

I
x
V

V

S

K

K

D

D

L

I

N

N

M

L

L

D

I

I

P

H

D

E

G

G

K

E

I

F

T

V

A

V

I

F

I

V

G

G

P

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

K

R

T

M

V

I

A

A

R

G

I

L

I

E

Q

T

A

I

K

T

S

S

G

G

S

D

V

L

F

F

L

I

D

G

G

E

K

K

Q

R

I

M

-

N

-

D

-

T

-

P

S

A

K

E

E

R

S

G

T

V

K

G

S

M

I

V

A

F

Q
|
Q

K

S

M

Y

A

A

I

L

L

Y

P

P

Q

H

S

-

P

-

D

-

A

-

P

-

E

-

G

-

L

L

T

S

I

V

G

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

L

F

-

P

-

Y

-

Q

-

R

-

G

-

M

-

G

-

K

K

L

L

N

A

D

G

K

A

D

K

R

K

N

E

V

V

I

I

E

N

W

Q

A

R

L

V

K

N

A

Q

T

V

G

A

-

E

-

V

-

L

-

Q

I

L

S

A

D

H

F

L

K

L

D

D

R
|
R

E

K

V

P

D

K

A
|
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

W

A

I

I

A

G

M

R

A

T

L

L

A

V

Q

A

E

E

T

P

D

S

I

V

I

F

L

L

D

D

E
|
E

P

P

T

L

T

S

Y

N

L

L

D

D

L

A

C

A

H

L

Q

R

L

V

E

Q

V

M

L

R

E

I

L

E

L

I

K

S

E

R

L

L

N

H

V

K

L

R

E

L

N

G

R

R

T

T

I

M

V

I

M

Y

V

V

I

T

H
|
H

D

D

L

Q

N

V

H

E

A

A

A

M

R

T

F

L

A

A

D

D

H

K

L

I

I

V

A

V

M

L

R

D

A

A

G

G

S

R

I

V

V

A

K

Q

S

V

G

G

N

K

G

P

K

L

E

E

V

L

M

Y

T

H

K

Y

P

P

V

A

L

D

R

R

E

F

V

V

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ I18), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (= S43), T43 (= T44), R128 (= R135), A132 (= A139), S134 (= S141), Q137 (= Q144)
binding tetrafluoroaluminate ion: S37 (≠ N38), G38 (= G39), K41 (= K42), Q81 (= Q77), S134 (= S141), G135 (= G142), G136 (= G143), E158 (= E165), H191 (= H198)
binding magnesium ion: S42 (= S43), Q81 (= Q77)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
30% identity, 83% coverage: 12:236/271 of query aligns to 11:229/371 of 3puvA

query
sites
3puvA

A

A

Y
x
W

G

G

D

E

Q

V

V

V

I
x
V

V

S

K

K

D

D

L

I

N

N

M

L

L

D

I

I

P

H

D

E

G

G

K

E

I

F

T

V

A

V

I

F

I

V

G

G

P

P

N

S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

K

R

T

M

V

I

A

A

R

G

I

L

I

E

Q

T

A

I

K

T

S

S

G

G

S

D

V

L

F

F

L

I

D

G

G

E

K

K

Q

R

I

M

-

N

-

D

-

T

-

P

S

A

K

E

E

R

S

G

T

V

K

G

S

M

I

V

A

F

Q
|
Q

K

S

M

Y

A

A

I

L

L

Y

P

P

Q

H

S

-

P

-

D

-

A

-

P

-

E

-

G

-

L

L

T

S

I

V

G

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

L

F

-

P

-

Y

-

Q

-

R

-

G

-

M

-

G

-

K

K

L

L

N

A

D

G

K

A

D

K

R

K

N

E

V

V

I

I

E

N

W

Q

A

R

L

V

K

N

A

Q

T

V

G

A

-

E

-

V

-

L

-

Q

I

L

S

A

D

H

F

L

K

L

D

D

R
|
R

E

K

V

P

D

K

A
|
A

L

L

S
|
S

G

G

Q
|
Q

R

R

Q

Q

R

R

V

V

W

A

I

I

A

G

M

R

A

T

L

L

A

V

Q

A

E

E

T

P

D

S

I

V

I

F

L

L

D

D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

L

A

C

A

H

L

Q

R

L

V

E

Q

V

M

L

R

E

I

L

E

L

I

K

S

E

R

L

L

N

H

V

K

L

R

E

L

N

G

R

R

T

T

I

M

V

I

M

Y

V

V

I

T

H

H

D

D

L

Q

N

V

H

E

A

A

A

M

R

T

F

L

A

A

D

D

H

K

L

I

I

V

A

V

M

L

R

D

A

A

G

G

S

R

I

V

V

A

K

Q

S

V

G

G

N

K

G

P

K

L

E

E

V

L

M

Y

T

H

K

Y

P

P

V

A

L

D

R

R

E

F

V

V

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ I18), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (= S43), T43 (= T44), R128 (= R135), A132 (= A139), S134 (= S141), Q137 (= Q144)
binding magnesium ion: S42 (= S43), Q81 (= Q77)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
30% identity, 83% coverage: 12:236/271 of query aligns to 11:229/374 of 2awnB

query
sites
2awnB

A

A

Y
x
W

G

G

D

E

Q

V

V

V

I
x
V

V

S

K

K

D

D

L

I

N

N

M

L

L

D

I

I

P

H

D

E

G

G

K

E

I

F

T

V

A

V

I

F

I

V

G

G

P

P

N
x
S

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

K

R

T

M

V

I

A

A

R

G

I

L

I

E

Q

T

A

I

K

T

S

S

G

G

S

D

V

L

F

F

L

I

D

G

G

E

K

K

Q

R

I

M

-

N

-

D

-

T

-

P

S

A

K

E

E

R

S

G

T

V

K

G

S

M

I

V

A

F

Q

Q

K

S

M

Y

A

A

I

L

L

Y

P

P

Q

H

S

-

P

-

D

-

A

-

P

-

E

-

G

-

L

L

T

S

I

V

G

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

L

F

-

P

-

Y

-

Q

-

R

-

G

-

M

-

G

-

K

K

L

L

N

A

D

G

K

A

D

K

R

K

N

E

V

V

I

I

E

N

W

Q

A

R

L

V

K

N

A

Q

T

V

G

A

-

E

-

V

-

L

-

Q

I

L

S

A

D

H

F

L

K

L

D

D

R

R

E

K

V

P

D

K

A

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

W

A

I

I

A

G

M

R

A

T

L

L

A

V

Q

A

E

E

T

P

D

S

I

V

I

F

L

L

D

D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

L

A

C

A

H

L

Q

R

L

V

E

Q

V

M

L

R

E

I

L

E

L

I

K

S

E

R

L

L

N

H

V

K

L

R

E

L

N

G

R

R

T

T

I

M

V

I

M

Y

V

V

I

T

H

H

D

D

L

Q

N

V

H

E

A

A

A

M

R

T

F

L

A

A

D

D

H

K

L

I

I

V

A

V

M

L

R

D

A

A

G

G

S

R

I

V

V

A

K

Q

S

V

G

G

N

K

G

P

K

L

E

E

V

L

M

Y

T

H

K

Y

P

P

V

A

L

D

R

R

E

F

V

V

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ I18), S37 (≠ N38), G38 (= G39), G40 (= G41), K41 (= K42), S42 (= S43), T43 (= T44)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
30% identity, 83% coverage: 12:236/271 of query aligns to 9:227/367 of 1q12A

query
sites
1q12A

A

A

Y
x
W

G

G

D

E

Q

V

V

V

I

V

V

S

K

K

D

D

L

I

N

N

M

L

L

D

I

I

P

H

D

E

G

G

K

E

I

F

T

V

A

V

I

F

I

V

G

G

P

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

K

R

T

M

V

I

A

A

R

G

I

L

I

E

Q

T

A

I

K

T

S

S

G

G

S

D

V

L

F

F

L

I

D

G

G

E

K

K

Q

R

I

M

-

N

-

D

-

T

-

P

S

A

K

E

E

R

S

G

T

V

K

G

S

M

I

V

A

F

Q

Q

K

S

M

Y

A

A

I

L

L

Y

P

P

Q

H

S

-

P

-

D

-

A

-

P

-

E

-

G

-

L

L

T

S

I

V

G

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

L

F

-

P

-

Y

-

Q

-

R

-

G

-

M

-

G

-

K

K

L

L

N

A

D

G

K

A

D

K

R

K

N

E

V

V

I

I

E

N

W

Q

A

R

L

V

K

N

A

Q

T

V

G

A

-

E

-

V

-

L

-

Q

I

L

S

A

D

H

F

L

K

L

D

D

R
|
R

E

K

V

P

D

K

A
|
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

W

A

I

I

A

G

M

R

A

T

L

L

A

V

Q

A

E

E

T

P

D

S

I

V

I

F

L

L

D

D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

L

A

C

A

H

L

Q

R

L

V

E

Q

V

M

L

R

E

I

L

E

L

I

K

S

E

R

L

L

N

H

V

K

L

R

E

L

N

G

R

R

T

T

I

M

V

I

M

Y

V

V

I

T

H

H

D

D

L

Q

N

V

H

E

A

A

A

M

R

T

F

L

A

A

D

D

H

K

L

I

I

V

A

V

M

L

R

D

A

A

G

G

S

R

I

V

V

A

K

Q

S

V

G

G

N

K

G

P

K

L

E

E

V

L

M

Y

T

H

K

Y

P

P

V

A

L

D

R

R

E

F

V

V

binding adenosine-5'-triphosphate: W10 (≠ Y13), S35 (≠ N38), G36 (= G39), C37 (= C40), G38 (= G41), K39 (= K42), S40 (= S43), T41 (= T44), R126 (= R135), A130 (= A139), S132 (= S141), G134 (= G143), Q135 (= Q144)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
31% identity, 83% coverage: 8:231/271 of query aligns to 7:227/241 of 4u00A

query
sites
4u00A

D

N

L

L

R

H

I

K

A

W

Y
x
F

G

G

D

P

Q

L

V

H

I
x
V

V

L

K

K

D

G

L

I

N

H

M

L

L

E

I

V

P

A

D

P

G

G

K

E

I

K

T

L

A

V

I

I

G

G

P

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

I

K

R

T

T

V

I

A

N

R

R

I

L

I

E

Q

D

A

F

K

Q

S

E

G

G

S

E

V

V

F

V

L

V

D

D

G

G

K

L

Q

S

I

V

S

K

K

D

E

D

-

R

S

A

T

L

K

R

S

E

I

I

A

R

Q

R

K

E

M

V

A

G

I

M

L

V

P

F

Q

Q

S

Q

P

F

D

N

A

L

P

F

E

P

G

H

L

M

T

T

I

V

G

L

E

E

L

N

V

V

S

T

Y

L

G

A

-

P

-

M

-

R

-

V

-

R

R

R

F

W

P

P

Y

R

Q

E

R

K

G

A

M

-

G

-

K

-

L

-

N

-

D

-

K

-

D

E

R

K

N

K

V

A

I

L

E

E

W

L

A

-

L

L

K

E

A

R

T

V

G

G

I

I

S

L

D

D

F

Q

K

A

D

R

R

K

E

Y

V

P

D

A

A

Q

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

W

A

I

I

A

A

M

R

A

A

L

L

A

A

Q

M

E

E

T

P

D

K

I

I

I

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

Y

A

L

L

D

D

L

P

C

E

H

M

Q

V

L

G

E

E

V

V

L

L

E

D

L

V

L

M

K

R

E

D

L

L

N

-

V

A

L

Q

E

G

N

G

R

M

T

T

I

M

V

V

M

V

V

V

I

T

H

H

D

E

L

M

N

G

H

F

A

A

A

R

R

E

F

V

A

A

D

D

H

R

L

V

I

V

A

F

M

M

R

D

A

G

G

G

S

Q

I

I

V

V

K

E

S

E

G

G

N

R

G

P

K

E

E

E

V

I

M

F

T

T

K

R

P

P

binding adenosine-5'-diphosphate: F12 (≠ Y13), V17 (≠ I18), S37 (≠ N38), G38 (= G39), S39 (≠ C40), G40 (= G41), K41 (= K42), S42 (= S43), T43 (= T44)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
30% identity, 83% coverage: 12:236/271 of query aligns to 12:230/369 of P19566

query
sites
P19566

A

A

Y

W

G

G

D

D

Q

V

V

V

I

V

V

S

K

K

D

D

L

I

N

N

M

L

L

D

I

I

P

H

D

D

G

G

K

E

I

F

T

V

A

V

I

F

I

V

G

G

P

P

N

S

G

G

C

C

G

G

K

K

S

S

T

T

V

L

L

L

K

R

T

M

V

I

A

A

R

G

I

L

I

E

Q

T

A

I

K

T

S

S

G

G

S

D

V

L

F

F

L

I

D

G

G

E

K

T

Q

R

I

M

-

N

-

D

-

I

-

P

S

A

K

E

E

R

S

G

T

V

K

G

S

M

I

V

A

F

Q

Q

K

S

M

Y

A

A

I
x
L

L

Y

P

P

Q

H

S

-

P

-

D

-

A

-

P

-

E

-

G

-

L

L

T

S

I

V

G

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

L

F

K

P

L

Y

A

Q

G

R

A

G

K

M

K

G

E

K

V

L

M

N

N

D

Q

K

R

D

V

R

N

N

Q

V

V

I

A

E

E

W

-

A

-

L

-

K

-

A

V

T

L

G

Q

I

L

S

A

D

H

F

L

K

L

D

E

R

R

E

K

V

P

D

K

A

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

W

A

I

I

A

G

M

R

A

T

L

L

A

V

Q

A

E

E

T

P

D

R

I

V

I

F

L

L

D

D

E

E

P
|
P

T

L

T

S

Y

N

L

L

D
|
D

L

A

C

A

H

L

Q

R

L

V

E

Q

V

M

L

R

E

I

L

E

L

I

K

S

E

R

L

L

N

H

V

K

L

R

E

L

N

G

R

R

T

T

I

M

V

I

M

Y

V

V

I

T

H

H

D

D

L

Q

N

V

H

E

A

A

A

M

R

T

F

L

A

A

D

D

H

K

L

I

I

V

A

V

M

L

R

D

A

A

G

G

S

R

I

V

V

A

K

Q

S

V

G

G

N

K

G

P

K

L

E

E

V

L

M

Y

T

H

K

Y

P

P

V

A

L

D

R

R

E

F

V

V

L86 (≠ I81) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P166) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D171) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

Q5M244 Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; EC 3.6.3.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
35% identity, 77% coverage: 21:230/271 of query aligns to 25:235/280 of Q5M244

query
sites
Q5M244

D

D

L

M

N

T

M

V

L

T

I

I

P

K

D

D

G

G

K

S

I

Y

T

T

A

A

I

F

I

I

G

G

P

H

N

T

G

G

C

S

G

G

K

K

S

S

T

T

V

I

L

M

K

Q

T

L

V

L

A

N

R

G

I

L

I

Y

Q

L

A

P

K

T

S

S

G

G

S

Q

V

V

F

K

L

V

D

D

G

D

K

T

Q

I

I

I

S

N

K

S

E

Q

S

S

T

K

-

N

-

K

-

E

-

I

K

K

S

P

I

I

A

R

Q

K

K

K

M

V

A

G

I

L

L

V

P

F

Q

Q

S

F

P

P

D

E

A

S

P
x
Q

-

L

E

F

G

A

L

E

T

T

I

V

G

L

E

E

L

D

V

I

S

A

Y

F

G

G

R

-

F

-

P

P

Y

Q

Q

N

R

F

G

G

M

V

G

S

K

K

L

E

N

E

D

A

K

E

D

Q

R

R

N

A

V

L

I

-

E

-

W

E

A

S

L

L

K

R

A

L

T

V

G

G

I

L

S

S

D

D

-

E

F

L

K

R

D

D

R

Q

E

N

V

P

D

F

A

D

L

L

S

S

G

G

Q

Q

R

M

Q

R

R

R

V

V

W

A

I

I

A

A

M

G

A

I

L

L

A

A

Q

M

E

Q

T

P

D

D

I

I

I

L

L

V

L

L

D

D

E
|
E

P

P

T

T

T

A

Y

G

L

L

D

D

L

P

C

Q

H

G

Q

R

L

K

E

E

V

L

L

M

E

S

L

L

L

F

K

K

E

Q

L

L

N

H

V

-

L

L

E

S

N

G

R

I

T

T

I

I

V

V

M

L

V

V

I

T

H

H

D

L

L

M

N

D

H

D

A

V

A

A

R

D

F

Y

A

A

D

T

H

A

L

V

I

N

A

V

M

M

R

E

A

K

G

G

S

R

I

L

V

V

K

L

S

S

G

G

N

T

G

P

K

K

E

D

V

V

M

F

T

Q

K

K

Q97 (≠ P89) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-90 in EcfA1.
E171 (= E165) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-163 in EcfA1.

5d3mA Folate ecf transporter: amppnp bound state (see paper)
32% identity, 78% coverage: 19:230/271 of query aligns to 23:233/280 of 5d3mA

query
sites
5d3mA

V

L

K

S

D

D

L

L

N

S

M

F

L

A

I

I

P

E

D

R

G

G

K

S

I

W

T

T

A

A

I

L

I

I

G

G

P

H

N
|
N

G
|
G

C

S

G

G

K
|
K

S
|
S

T

T

V

V

L

S

K

K

T

L

V

I

A

N

R

G

I

L

Q

A

A

P

K

D

S

D

-

L

-

D

-

K

G

S

S

S

V

I

F

T

L

V

D

D

G

G

K

V

Q

K

I

L

S

G

K

A

E

D

S

T

T

V

K

W

S

E

I

V

A

R

Q

E

K

K

M

V

A

G

I

I

L

V

P

F

Q

Q

S

N

P

P

D

D

A

N

P

Q

-

F

E

V

G

G

L

A

T

T

I

V

G

S

E

D

L

D

V

V

S

A

Y

F

G

G

-

L

-

E

-

N

R

R

F

A

P

V

Y

P

Q

R

R

P

G

E

M

M

G

L

K

K

L

-

N

-

D

-

K

-

D

-

R

-

N

-

V

I

I

V

E

A

W

Q

A

A

L

V

K

A

A

D

T

V

G

G

I

M

S

A

D

D

F

Y

K

A

D

D

R

S

E

E

V

P

D

S

A

N

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

V

V

W

A

I

I

A

A

M

G

A

I

L

L

A

A

Q

V

E

K

T

P

D

Q

I

V

I

I

L

I

L

L

D

D

E

E

P

S

T

T

T

S

Y

M

L

L

D

D

L

P

C

E

H

G

Q

K

L

E

E

Q

V

I

L

L

E

D

L

L

L

V

K

R

E

K

L

I

N

K

V

E

L

D

E

N

N

N

R

L

T

T

I

V

V

I

M

S

V

I

I

T

H

H

D

D

L

L

N

E

H

E

A

A

R

G

F

-

A

A

D

D

H

Q

L

V

I

L

A

V

M

L

R

D

A

D

G

G

S

Q

I

L

V

L

K

D

S

Q

G

G

N

K

G

P

K

E

E

E

V

I

M

F

T

P

K

K

binding phosphoaminophosphonic acid-adenylate ester: N42 (= N38), G43 (= G39), K46 (= K42), S47 (= S43)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

1f3oA Crystal structure of mj0796 atp-binding cassette (