SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_066199294.1 NCBI__GCF_002835735.1:WP_066199294.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 87% coverage: 42:465/485 of query aligns to 40:463/467 of 7qh2C

query
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7qh2C

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binding flavin-adenine dinucleotide: V73 (≠ A76), G75 (= G78), S76 (≠ A79), G77 (= G80), T78 (= T81), G79 (= G82), L80 (= L83), A83 (≠ G86), C84 (≠ A87), P137 (= P140), G138 (≠ S141), E139 (≠ S142), A142 (≠ C146), T143 (= T147), G146 (= G150), N147 (= N151), S149 (≠ A153), T150 (≠ E154), A152 (= A156), G153 (= G157), E203 (= E207), G204 (= G208), I209 (≠ V213), E422 (= E424), H423 (= H425)
binding fe (iii) ion: H377 (= H380), H384 (= H387), E422 (= E424)

P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 86% coverage: 48:466/485 of query aligns to 40:456/459 of P9WIT1

query
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P9WIT1

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K354 (≠ E359) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
29% identity, 88% coverage: 42:466/485 of query aligns to 33:455/455 of 8jduA

query
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E

V

E

F

V

K

G

D

P

E

V

E

G

I

V

Q

E

A

T

Q

M

T

R

D

Q

I

L

R

K

D

N

V

T

F

L

N

D

P

P

D

R

N

G

L

L

L

M

N
|
N

A

P

G

G

K

K

L

V

F

L

binding 2-oxopentanoic acid: R318 (= R331), W322 (≠ F335), H369 (= H380), H376 (= H387), H414 (= H425)
binding flavin-adenine dinucleotide: P68 (≠ A76), G70 (= G78), T71 (≠ A79), G72 (= G80), T73 (= T81), G74 (= G82), G78 (= G86), V79 (≠ A87), L90 (≠ M98), P132 (= P140), G133 (≠ S141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (≠ E154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (≠ V213), W322 (≠ F335), E413 (= E424), N450 (= N461)
binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E424)

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
28% identity, 88% coverage: 42:466/485 of query aligns to 33:455/455 of 8jdxA

query
sites
8jdxA

T

S

I

M

H

H

K

R

A

C

T

Q

P

P

-

P

K

D

A

A

V

V

F

W

P

P

E

Q

N

N

T

V

E

D

Q

Q

V

V

A

S

K

R

I

V

V

A

K

S

Y

L

C

C

H

Y

D

N

H

Q

S

G

L

V

P

P

F

I

L

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

L

V

N

Q

G

G

E

G

V

V

I

C

I

I

S

N

M
x
L

V

T

K

H

M

M

K

D

K

Q

L

I

L

T

S

E

V

L

D

N

L

T

E

E

N

D

R

F

R

S

A

V

V

V

E

E

P

P

G

G

F

V

V

T

N

R

L

K

K

A

L

L

T

N

N

T

T

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

C

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

A
|
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

N

D

G

G

D

R

V

L

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

N

H

G

Y

I

R

A

K

D

S

E

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

I

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

R

R

I

L

L

H

K

P

N

A

P

P

E

E

S

A

K

T

Q

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

K

S

V

V

E

Q

D

A

G

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

S

Q

A

A

G

A

I

V

V

P

P

V

A

A

A

R

L

I

E

E

M

F

M

L

D

D

K

D

T

V

A

M

I

M

E

D

G

A

V

C

E

N

A

R

A

H

A

S

F

L

P

N

V

C

G

-

H

-

P

-

K

-

D

P

I

V

E

A

A

P

L

T

L

L

L

F

I

L

E

E

V

F

D

H

G

G

I

S

E

Q

A

Q

G

T

I

L

G

A

E

E

Q

Q

I

L

E

Q

Q

R

I

T

L

E

Q

A

V

I

C

T

R

Q

N

D

Q

N

N

G

V

G

R

S

E

H

V

F

R

S

A

W

A

A

K

A

D

-

E

-

Q

E

E

K

R

R

A

N

A

E

W

L

W

W

A

A

N

A

R
|
R

K

H

T

N

G

A

F
x
W

G

Y

A

A

M

A

G

L

A

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

L

Y

V

S

Q

T

D

D

G

V

V

C

I

V

P

P

R

I

S

S

K

R

L

L

P

P

E

E

V

I

L

L

S

V

K

E

I

T

N

K

K

E

I

E

S

I

E

K

E

A

S

S

G

K

L

L

R

T

I

G

A

A

N

I

I

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

V

L

L

L

F

V

D

D

A

P

R

D

I

D

P

A

G

E

E

E

T

Q

E

R

K

R

A

V

M

K

E

A

A

F

G

A

S

E

A

N

C

L

L

G

K

R

V

R

C

A

A

L

D

A

V

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

E

Q

E

L

M

L

R

Q

F

E

V

E

F

V

K

G

D

P

E

V

E

G

I

V

Q

E

A

T

Q

M

T

R

D

Q

I

L

R

K

D

N

V

T

F

L

N

D

P

P

D

R

N

G

L

L

L

M

N
|
N

A

P

G

G

K

K

L

V

F

L

binding flavin-adenine dinucleotide: P68 (≠ A76), G70 (= G78), T71 (≠ A79), G72 (= G80), T73 (= T81), G74 (= G82), G78 (= G86), V79 (≠ A87), L90 (≠ M98), P132 (= P140), G133 (≠ S141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (≠ E154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (≠ V213), W322 (≠ F335), E413 (= E424), H414 (= H425), N450 (= N461)
binding 3-methyl-2-oxobutanoic acid: R318 (= R331), H369 (= H380), H376 (= H387), H414 (= H425)
binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E424)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
28% identity, 88% coverage: 42:466/485 of query aligns to 33:454/454 of 8jdvA

query
sites
8jdvA

T

S

I

M

H

H

K

R

A

C

T

Q

P

P

-

P

K

D

A

A

V

V

F

W

P

P

E

Q

N

N

T

V

E

D

Q

Q

V

V

A

S

K

R

I

V

V

A

K

S

Y

L

C

C

H

Y

D

N

H

Q

S

G

L

V

P

P

F

I

L

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L
x
V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

L

V

N

Q

G

G

E

G

V

V

I

C

I

I

S

N

M
x
L

V

T

K

H

M

M

K

D

K

Q

L

I

L

T

S

E

V

L

D

N

L

T

E

E

N

D

R

F

R

S

A

V

V

V

E

E

P

P

G

G

F

V

V

T

N

R

L

K

K

A

L

L

T

N

N

T

T

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

C

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

A
|
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

N

D

G

G

D

R

V

L

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

N

H

G

Y

I

R

A

K

D

S

E

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

I

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

R

R

I

L

L

H

K

P

N

A

P

P

E

E

S

A

K

T

Q

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

K

S

V

V

E

Q

D

A

G

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

S

Q

A

A

G

A

I

V

V

P

P

V

A

A

A

R

L

I

E

E

M

F

M

L

D

D

K

D

T

V

A

M

I

M

E

D

G

A

V

C

E

N

A

R

A

-

F

-

P

-

V

-

G

-

H

H

P

S

K

K

-

L

-

N

-

C

D

P

I

V

E

A

A

P

L

T

L

L

L

F

I

L

E

E

V

F

D

H

G

G

I

S

E

Q

A

Q

G

T

I

L

G

A

E

E

Q

Q

I

L

E

Q

Q

R

I

T

L

E

Q

A

V

I

C

T

R

Q

N

D

Q

N

N

G

V

G

R

S

E

H

V

F

R

S

A

A

A

E

K

K

D

-

E

-

Q

-

E

-

R

R

A

N

A

E

W

L

W

W

A

A

N

A

R
|
R

K

H

T

N

G

A

F
x
W

G
x
Y

A

A

M

A

G

L

A

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

L

Y

V

S

Q

T

D

D

G

V

V

C

I

V

P

P

R

I

S

S

K

R

L

L

P

P

E

E

V

I

L

L

S

V

K

E

I

T

N

K

K

E

I

E

S

I

E

K

E

A

S

S

G

K

L

L

R

T

I

G

A

A

N

I

I

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

V

L

L

L

F

V

D

D

A

P

R

D

I

D

P

A

G

E

E

E

T

Q

E

R

K

R

A

V

M

K

E

A

A

F

G

A

S

E

A

N

C

L

L

G

K

R

V

R

C

A

A

L

D

A

V

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

E

Q

E

L

M

L

R

Q

F

E

V

E

F

V

K

G

D

P

E

V

E

G

I

V

Q

E

A

T

Q

M

T

R

D

Q

I

L

R

K

D

N

V

T

F

L

N

D

P

P

D

R

N

G

L

L

L

M

N
|
N

A

P

G

G

K

K

L

V

F

L

binding 2-Ketohexanoic acid: V75 (≠ L83), R317 (= R331), W321 (≠ F335), H368 (= H380), H375 (= H387), H413 (= H425)
binding flavin-adenine dinucleotide: P68 (≠ A76), G70 (= G78), T71 (≠ A79), G72 (= G80), T73 (= T81), G74 (= G82), G78 (= G86), V79 (≠ A87), L90 (≠ M98), P132 (= P140), G133 (≠ S141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (≠ E154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (≠ V213), W321 (≠ F335), Y322 (≠ G336), E412 (= E424), H413 (= H425), N449 (= N461)
binding manganese (ii) ion: H368 (= H380), H375 (= H387), E412 (= E424)

8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
28% identity, 88% coverage: 42:466/485 of query aligns to 33:455/455 of 8jdeA

query
sites
8jdeA

T

S

I

M

H

H

K

R

A

C

T

Q

P

P

-

P

K

D

A

A

V

V

F

W

P

P

E

Q

N

N

T

V

E

D

Q

Q

V

V

A

S

K

R

I

V

V

A

K

S

Y

L

C

C

H

Y

D

N

H

Q

S

G

L

V

P

P

F

I

L

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

L

V

N

Q

G

G

E

G

V

V

I

C

I

I

S

N

M
x
L

V

T

K

H

M

M

K

D

K

Q

L

I

L

T

S

E

V

L

D

N

L

T

E

E

N

D

R

F

R

S

A

V

V

V

E

E

P

P

G

G

F

V

V

T

N

R

L

K

K

A

L

L

T

N

N

T

T

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

C

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

A
|
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

N

D

G

G

D

R

V

L

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

N

H

G

Y

I

R

A

K

D

S

E

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

I

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

R

R

I

L

L

H

K

P

N

A

P

P

E

E

S

A

K

T

Q

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

K

S

V

V

E

Q

D

A

G

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

S

Q

A

A

G

A

I

V

V

P

P

V

A

A

A

R

L

I

E

E

M

F

M

L

D

D

K

D

T

V

A

M

I

M

E

D

G

A

V

C

E

N

A

R

A

-

F

-

P

-

V

-

G

-

H

H

P

S

K

K

-

L

-

N

-

C

D

P

I

V

E

A

A

P

L

T

L

L

L

F

I

L

E

E

V

F

D

H

G

G

I

S

E

Q

A

Q

G

T

I

L

G

A

E

E

Q

Q

I

L

E

Q

Q

R

I

T

L

E

Q

A

V

I

C

T

R

Q

N

D

Q

N

N

G

V

G

R

S

E

H

V

F

R

S

A

W

A

A

K

A

D

E

E

K

Q

N

E

E

R

-

A

-

W

L

W

W

A

A

N

A

R
|
R

K

H

T

N

G

A

F
x
W

G

Y

A

A

M

A

G

L

A

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

L

Y

V

S

Q

T

D

D

G

V

V

C

I

V

P

P

R

I

S

S

K

R

L

L

P

P

E

E

V

I

L

L

S

V

K

E

I

T

N

K

K

E

I

E

S

I

E

K

E

A

S

S

G

K

L

L

R

T

I

G

A

A

N

I

I

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

V

L

L

L

F

V

D

D

A

P

R

D

I

D

P

A

G

E

E

E

T

Q

E

R

K

R

A

V

M

K

E

A

A

F

G

A

S

E

A

N

C

L

L

G

K

R

V

R

C

A

A

L

D

A

V

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

E

Q

E

L

M

L

R

Q

F

E

V

E

F

V

K

G

D

P

E

V

E

G

I

V

Q

E

A

T

Q

M

T

R

D

Q

I

L

R

K

D

N

V

T

F

L

N

D

P

P

D

R

N

G

L

L

L

M

N
|
N

A

P

G

G

K

K

L

V

F

L

binding flavin-adenine dinucleotide: P68 (≠ A76), G70 (= G78), T71 (≠ A79), G72 (= G80), T73 (= T81), G74 (= G82), G78 (= G86), V79 (≠ A87), L90 (≠ M98), P132 (= P140), G133 (≠ S141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (≠ E154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (≠ V213), W322 (≠ F335), E413 (= E424), H414 (= H425), N450 (= N461)
binding lactic acid: R318 (= R331), H369 (= H380), H376 (= H387), H414 (= H425)
binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E424)

8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
28% identity, 88% coverage: 42:466/485 of query aligns to 33:454/454 of 8jdzA

query
sites
8jdzA

T

S

I

M

H

H

K

R

A

C

T

Q

P

P

-

P

K

D

A

A

V

V

F

W

P

P

E

Q

N

N

T

V

E

D

Q

Q

V

V

A

S

K

R

I

V

V

A

K

S

Y

L

C

C

H

Y

D

N

H

Q

S

G

L

V

P

P

F

I

L

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

L

V

N

Q

G

G

E

G

V

V

I

C

I

I

S

N

M
x
L

V

T

K

H

M

M

K

D

K

Q

L

I

L

T

S

E

V

L

D

N

L

T

E

E

N

D

R

F

R

S

A

V

V

V

E

E

P

P

G

G

F

V

V

T

N

R

L

K

K

A

L

L

T

N

N

T

T

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

C

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

A
|
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

N

D

G

G

D

R

V

L

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

N

H

G

Y

I

R

A

K

D

S

E

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

I

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

R

R

I

L

L

H

K

P

N

A

P

P

E

E

S

A

K

T

Q

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

K

S

V

V

E

Q

D

A

G

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

S

Q

A

A

G

A

I

V

V

P

P

V

A

A

A

R

L

I

E

E

M

F

M

L

D

D

K

D

T

V

A

M

I

M

E

D

G

A

V

C

E

N

A

R

A

H

A

S

F

L

P

N

V

C

G

-

H

-

P

-

K

-

D

P

I

V

E

A

A

P

L

T

L

L

L

F

I

L

E

E

V

F

D

H

G

G

I

S

E

Q

A

Q

G

T

I

L

G

A

E

E

Q

Q

I

L

E

Q

Q

R

I

T

L

E

Q

A

V

I

C

T

R

Q

N

D

Q

N

N

G

V

G

R

S

E

H

V

F

R

S

A

W

A

A

K

A

D

-

E

-

Q

E

E

K

R

R

A

N

A

E

W

L

W

W

A

A

N

A

R
|
R

K

H

T

N

G

A

F
x
W

G

Y

A

A

M

A

G

L

A

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

L

Y

V

S

Q

T

D

D

G

V

V

C

I

V

P

P

R

I

S

S

K

R

L

L

P

P

E

E

V

I

L

L

S

V

K

E

I

T

N

K

K

E

I

E

S

I

E

K

E

A

S

S

G

K

L

L

R

T

I

G

A

A

N

I

I

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

V

L

L

L

F

V

D

D

A

P

R

D

I

-

P

-

G

A

E

E

T

E

E

Q

K

R

A

R

M

V

E

K

A

A

G

F

S

A

A

E

C

N

L

L

-

G

K

R

V

R

C

A

A

L

D

A

V

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

E

Q

E

L

M

L

R

Q

F

E

V

E

F

V

K

G

D

P

E

V

E

G

I

V

Q

E

A

T

Q

M

T

R

D

Q

I

L

R

K

D

N

V

T

F

L

N

D

P

P

D

R

N

G

L

L

L

M

N
|
N

A

P

G

G

K

K

L

V

F

L

binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R331), W322 (≠ F335), H369 (= H380), H376 (= H387), H413 (= H425)
binding flavin-adenine dinucleotide: P68 (≠ A76), G70 (= G78), T71 (≠ A79), G72 (= G80), T73 (= T81), G74 (= G82), G78 (= G86), V79 (≠ A87), L90 (≠ M98), P132 (= P140), G133 (≠ S141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (≠ E154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (≠ V213), W322 (≠ F335), E412 (= E424), H413 (= H425), N449 (= N461)
binding manganese (ii) ion: H369 (= H380), H376 (= H387), E412 (= E424)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
28% identity, 88% coverage: 42:466/485 of query aligns to 33:454/454 of 8jdyA

query
sites
8jdyA

T

S

I

M

H

H

K

R

A

C

T

Q

P

P

-

P

K

D

A

A

V

V

F

W

P

P

E

Q

N

N

T

V

E

D

Q

Q

V

V

A

S

K

R

I

V

V

A

K

S

Y

L

C

C

H

Y

D

N

H

Q

S

G

L

V

P

P

F

I

L

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

L

V

N

Q

G

G

E

G

V

V

I

C

I

I

S

N

M
x
L

V

T

K

H

M

M

K

D

K

Q

L

I

L

T

S

E

V

L

D

N

L

T

E

E

N

D

R

F

R

S

A

V

V

V

E

E

P

P

G

G

F

V

V

T

N

R

L

K

K

A

L

L

T

N

N

T

T

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

C

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

A
|
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

N

D

G

G

D

R

V

L

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

N

H

G

Y

I

R

A

K

D

S

E

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

I

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

R

R

I

L

L

H

K

P

N

A

P

P

E

E

S

A

K

T

Q

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

K

S

V

V

E

Q

D

A

G

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

S

Q

A

A

G

A

I

V

V

P

P

V

A

A

A

R

L

I

E

E

M

F

M

L

D

D

K

D

T

V

A

M

I

M

E

D

G

A

V

C

E

N

A

R

A

H

A

S

F

L

P

N

V

C

G

-

H

-

P

-

K

-

D

P

I

V

E

A

A

P

L

T

L

L

L

F

I

L

E

E

V

F

D

H

G

G

I

S

E

Q

A

Q

G

T

I

L

G

A

E

E

Q

Q

I

L

E

Q

Q

R

I

T

L

E

Q

A

V

I

C

T

R

Q

N

D

Q

N

N

G

V

G

R

S

E

H

V

F

R

S

A

W

A

A

K

A

D

-

E

-

Q

E

E

K

R

R

A

N

A

E

W

L

W

W

A

A

N

A

R
|
R

K

H

T

N

G

A

F
x
W

G

Y

A

A

M

A

G

L

A

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

L

Y

V
x
S

Q

T

D

D

G

V

V

C

I

V

P

P

R

I

S

S

K

R

L

L

P

P

E

E

V

I

L

L

S

V

K

E

I

T

N

K

K

E

I

E

S

I

E

K

E

A

S

S

G

K

L

L

R

T

I

G

A

A

N

I

I

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

V

L

L

L

F

V

D

D

A

P

R

D

I

-

P

-

G

A

E

E

T

E

E

Q

K

R

A

R

M

V

E

K

A

A

G

F

S

A

A

E

C

N

L

L

-

G

K

R

V

R

C

A

A

L

D

A

V

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

E

Q

E

L

M

L

R

Q

F

E

V

E

F

V

K

G

D

P

E

V

E

G

I

V

Q

E

A

T

Q

M

T

R

D

Q

I

L

R

K

D

N

V

T

F

L

N

D

P

P

D

R

N

G

L

L

L

M

N
|
N

A

P

G

G

K

K

L

V

F

L

binding 2-oxo-4-methylpentanoic acid: R318 (= R331), W322 (≠ F335), S336 (≠ V347), H369 (= H380), H376 (= H387), H413 (= H425)
binding flavin-adenine dinucleotide: P68 (≠ A76), G70 (= G78), T71 (≠ A79), G72 (= G80), T73 (= T81), G74 (= G82), G78 (= G86), V79 (≠ A87), L90 (≠ M98), P132 (= P140), G133 (≠ S141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (≠ E154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (≠ V213), E412 (= E424), N449 (= N461)
binding manganese (ii) ion: H369 (= H380), H376 (= H387), E412 (= E424)

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
28% identity, 88% coverage: 42:466/485 of query aligns to 33:455/455 of 8jdtA

query
sites
8jdtA

T

S

I

M

H

H

K

R

A

C

T

Q

P

P

-

P

K

D

A

A

V

V

F

W

P

P

E

Q

N

N

T

V

E

D

Q

Q

V

V

A

S

K

R

I

V

V

A

K

S

Y

L

C

C

H

Y

D

N

H

Q

S

G

L

V

P

P

F

I

L

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

L

V

N

Q

G

G

E

G

V

V

I

C

I

I

S

N

M
x
L

V

T

K

H

M

M

K

D

K

Q

L

I

L

T

S

E

V

L

D

N

L

T

E

E

N

D

R

F

R

S

A

V

V

V

E

E

P

P

G

G

F

V

V

T

N

R

L

K

K

A

L

L

T

N

N

T

T

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

C

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

A
|
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

N

D

G

G

D

R

V

L

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

N

H

G

Y

I

R

A

K

D

S

E

A

P

A

G

G

Y

Y

D

N

L

L

L

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G

G

L

L

I

F

T

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G

G

S

S

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|
E

G
|
G

T

T

L

L

G

G

I

I

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x
I

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I

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|
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binding 2-ketobutyric acid: R318 (= R331), H369 (= H380), H376 (= H387), H414 (= H425)
binding flavin-adenine dinucleotide: P68 (≠ A76), G70 (= G78), T71 (≠ A79), G72 (= G80), T73 (= T81), G74 (= G82), G78 (= G86), V79 (≠ A87), L90 (≠ M98), P132 (= P140), G133 (≠ S141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (≠ E154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (≠ V213), W322 (≠ F335), E413 (= E424), H414 (= H425), N450 (= N461)
binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E424)

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
28% identity, 88% coverage: 42:466/485 of query aligns to 33:456/456 of 8jdsA

query
sites
8jdsA

T

S

I

M

H

H

K

R

A

C

T

Q

P

P

-

P

K

D

A

A

V

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|
G

A
x
T

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|
G

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|
T

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|
G

L

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G

G

G
|
G

A
x
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|
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|
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