SitesBLAST
Comparing WP_066324538.1 NCBI__GCF_900100165.1:WP_066324538.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
33% identity, 79% coverage: 30:235/261 of query aligns to 30:236/265 of P07821
- K50 (= K50) mutation to Q: Lack of activity.
- D172 (= D172) mutation to E: Lack of activity.
- E173 (= E173) mutation to A: Lack of activity.
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 84% coverage: 26:244/261 of query aligns to 32:254/378 of P69874
- F45 (≠ L39) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (≠ I48) mutation to T: Loss of ATPase activity and transport.
- L60 (= L54) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (= L70) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (≠ T135) mutation to M: Loss of ATPase activity and transport.
- D172 (= D172) mutation to N: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 26 C→A: Lower ATPase activity and transport efficiency.
- 27 F→L: Lower ATPase activity and transport efficiency.
- 276 C→A: Lower ATPase activity and transport efficiency.
- 297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.
8wm7C Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
36% identity, 77% coverage: 25:225/261 of query aligns to 21:216/658 of 8wm7C
Sites not aligning to the query:
8wm7D Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
34% identity, 84% coverage: 4:222/261 of query aligns to 1:212/257 of 8wm7D
8w9mC Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
36% identity, 77% coverage: 25:225/261 of query aligns to 21:216/256 of 8w9mC
- binding adenosine-5'-triphosphate: S42 (≠ N46), G43 (= G47), G45 (= G49), K46 (= K50), S47 (= S51), T48 (= T52), Q83 (≠ K85), K132 (= K143), E136 (= E147), S138 (= S149), G140 (= G151), H195 (= H206)
- binding magnesium ion: S47 (= S51), Q83 (≠ K85)
Sites not aligning to the query:
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
29% identity, 78% coverage: 25:228/261 of query aligns to 16:213/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: S37 (≠ N46), G38 (= G47), C39 (≠ I48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), Q81 (≠ T92), R128 (≠ K143), A132 (≠ E147), S134 (= S149), G136 (= G151), Q137 (= Q152), E158 (= E173), H191 (= H206)
- binding magnesium ion: S42 (= S51), Q81 (≠ T92)
Sites not aligning to the query:
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
29% identity, 78% coverage: 25:228/261 of query aligns to 16:213/371 of 3puxA
- binding adenosine-5'-diphosphate: G38 (= G47), C39 (≠ I48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), R128 (≠ K143), S134 (= S149), Q137 (= Q152)
- binding beryllium trifluoride ion: S37 (≠ N46), G38 (= G47), K41 (= K50), Q81 (≠ T92), S134 (= S149), G136 (= G151), H191 (= H206)
- binding magnesium ion: S42 (= S51), Q81 (≠ T92)
Sites not aligning to the query:
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
29% identity, 78% coverage: 25:228/261 of query aligns to 16:213/371 of 3puwA
- binding adenosine-5'-diphosphate: V17 (= V26), G38 (= G47), C39 (≠ I48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), R128 (≠ K143), A132 (≠ E147), S134 (= S149), Q137 (= Q152)
- binding tetrafluoroaluminate ion: S37 (≠ N46), G38 (= G47), K41 (= K50), Q81 (≠ T92), S134 (= S149), G135 (≠ D150), G136 (= G151), E158 (= E173), H191 (= H206)
- binding magnesium ion: S42 (= S51), Q81 (≠ T92)
Sites not aligning to the query:
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
29% identity, 78% coverage: 25:228/261 of query aligns to 16:213/371 of 3puvA
- binding adenosine-5'-diphosphate: V17 (= V26), G38 (= G47), C39 (≠ I48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), R128 (≠ K143), A132 (≠ E147), S134 (= S149), Q137 (= Q152)
- binding magnesium ion: S42 (= S51), Q81 (≠ T92)
Sites not aligning to the query:
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
29% identity, 78% coverage: 25:228/261 of query aligns to 17:214/371 of P68187
- A85 (≠ L95) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ L120) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V128) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ A131) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ K133) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ E138) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G151) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D172) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
29% identity, 78% coverage: 25:228/261 of query aligns to 14:211/367 of 1q12A
- binding adenosine-5'-triphosphate: S35 (≠ N46), G36 (= G47), C37 (≠ I48), G38 (= G49), K39 (= K50), S40 (= S51), T41 (= T52), R126 (≠ K143), A130 (≠ E147), S132 (= S149), G134 (= G151), Q135 (= Q152)
Sites not aligning to the query:
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
29% identity, 78% coverage: 25:228/261 of query aligns to 16:213/374 of 2awnB
Sites not aligning to the query:
8w9mD Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
34% identity, 83% coverage: 6:222/261 of query aligns to 1:210/256 of 8w9mD
- binding adenosine-5'-triphosphate: Y12 (= Y17), H40 (≠ A45), S41 (≠ N46), G42 (= G47), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52), Q82 (≠ L91), Q135 (≠ E147), S137 (= S149), G139 (= G151), M140 (≠ Q152), H194 (= H206)
- binding magnesium ion: S46 (= S51), Q82 (≠ L91)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
29% identity, 78% coverage: 25:228/261 of query aligns to 17:214/369 of P19566
- L86 (≠ P96) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P174) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D179) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
29% identity, 77% coverage: 20:221/261 of query aligns to 14:202/353 of 1vciA
7arlD Lolcde in complex with lipoprotein and adp (see paper)
29% identity, 81% coverage: 7:218/261 of query aligns to 2:213/222 of 7arlD
P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
30% identity, 81% coverage: 7:218/261 of query aligns to 5:216/233 of P75957
- G42 (= G44) mutation to D: Loss of lipoprotein release when overexpressed.
7mdyC Lolcde nucleotide-bound
29% identity, 81% coverage: 7:218/261 of query aligns to 2:213/226 of 7mdyC
- binding adp orthovanadate: Y12 (= Y17), G42 (= G47), S43 (≠ I48), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52), Q91 (≠ T92), H138 (≠ K143), E142 (= E147), S144 (= S149), G145 (≠ D150), G146 (= G151), E168 (= E173), N172 (≠ H177), H201 (= H206)
- binding magnesium ion: S46 (= S51), Q91 (≠ T92)
7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
29% identity, 81% coverage: 7:218/261 of query aligns to 4:215/229 of 7v8iD
- binding phosphoaminophosphonic acid-adenylate ester: V23 (= V26), S43 (≠ N46), G44 (= G47), G46 (= G49), K47 (= K50), S48 (= S51), T49 (= T52), Q93 (≠ T92), R137 (≠ L140), H140 (≠ K143), E144 (= E147), S146 (= S149), G148 (= G151), E149 (≠ Q152), H203 (= H206)
- binding magnesium ion: S48 (= S51), Q93 (≠ T92)
3vx4D Crystal structure of the nucleotide-binding domain of s. Mutans coma, a bifunctional atp-binding cassette transporter involved in the quorum-sensing pathway (see paper)
29% identity, 92% coverage: 5:244/261 of query aligns to 5:239/240 of 3vx4D
- binding adenosine-5'-triphosphate: Y17 (= Y17), T23 (≠ V26), S43 (≠ N46), G44 (= G47), G46 (= G49), K47 (= K50), T48 (≠ S51), T49 (= T52), Q89 (≠ T92), G144 (vs. gap), I145 (vs. gap), S146 (vs. gap), G148 (= G151), Q149 (= Q152), H200 (= H206)
- binding magnesium ion: T48 (≠ S51), Q89 (≠ T92)
Query Sequence
>WP_066324538.1 NCBI__GCF_900100165.1:WP_066324538.1
MSNSDSILQAEQISIGYTHKKEQNIVASNIDLKLEKGKLVALIGANGIGKSTLLRTITGI
QKTISGTVLLNKKDIHRMNPLALAKNLSVVLTEKLPPSNLSVFELVALGRQPYTNWIGTL
TEEDIEKVNQALKLTQIEHLATKRHHEISDGQLQKVLVARALAQDTPLIVLDEPTTHLDL
LHKVSLFRLLKKLTQETQKCILFSTHDIDLAIHLSDEMIIMTPETVVQDQPCNLISKGIF
GNLFKDEHITFDAEKGRFIFS
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory